Mayonnaise Samples Research Articles

Development of Prangos ferulacea essential oil based nanoemulsions/pickering emulsion and examination of their performance in mayonnaise shelf life

The increasing demand to replace chemical antimicrobial agents with natural compounds leads to the use of some plant extracts and essential oils (EOs) as natural preservatives. Nanoemulsion (NE) with Tween 80 as wall material and Pickering emulsions (PE) stabilized with carbohydrate (Arabic gum (AG)) or protein (sodium caseinate (SC)) wall materials. First of all, the PEO was obtained from two parts of plant: flowers and leave, and characterized by GC-MS and the IC50 index measurement. The Prangos ferulacea essential oil (PEO) extracted from leave was selected for the next tests due to slightly higher antioxidant activity compared to the flower's EO. The leave's PEO was encapsulated in three forms: nanoemulsion (NE) with Tween 80 as wall material and Pickering emulsions (PE) stabilized with carbohydrate (gum Arabic (GA)) or protein (sodium caseinate (SC)) wall materials. The NE had the smallest particle size and the highest antioxidant and antimicrobial (S. aureus and E. coli) activity. AG-stabilized PE recorded the highest encapsulation efficiency (85.37 ± 1.97 %). The occurring of new chemical interactions between PEO and wall materials and uniformly shaped PE microcapsules were approved by FT-IR and FE-SEM tests, respectively. Free and encapsulated PEO were added to the mayonnaise, and the samples were analyzed during storage at 4 °C for 60 days. Peroxide value and TBA index gradually increased for all mayonnaise samples, but this increase was lower for samples containing NE and SC-stabilized PEO capsules. In general, carriers stabilized with the simultaneous application of protein and carbohydrate performed better compared to their single application in sensory tests in mayonnaise.

Investigation of benzoic acid and sorbic acid concentrations in tomato paste, pepper paste, ketchup, mayonnaise, and barbeque sauce samples by headspace gas chromatography-mass spectrometry.

In this study, it was aimed at investigating benzoic acid (BA) and sorbic acid (SoA) concentrations in tomato paste, pepper paste, ketchup, mayonnaise, and barbeque sauce samples by a validated static headspace gas chromatography-mass spectrometry (GC-MS) method. Salicylic acid (SalA) was used as internal standard and the measurements were conducted in the wide linear concentration ranges of BA and SoA which were 2.5-5000 and 12.5-5000, respectively. The limit of detections (LODs) were determined to be 1.5 and 4.5 mg/kg while the limit of quantifications (LOQs) were 2.5 and 12.5 mg/kg for BA and SoA, respectively. The average recovery% values of BA and SoA were found to be 98.5% and 98.7% in an open tomato paste sample while these values were 98.7% and 100.3% in a mayonnaise sample, respectively. Accuracy of the proposed method was confirmed by statistically (significance test) evaluating excellent recovery values. In real samples, while the results of the canned tomato pastes and industrial sauce samples were found suitable, BA and SoA ​​were determined in some tomato and pepper paste products sold under the traditional or homemade name although use of the preservatives in the pastes were prohibited. It is vital for public health to prevent adulteration in pastes which is indispensable for Turkish cuisine as well as prevalently consumed in the world. Therefore, the proposed method can be used in food control laboratories due to its reliability and consumption of much less toxic chemical reagents.

Investigating the physicochemical, rheological, and sensory properties of low-fat mayonnaise prepared with amaranth protein as an egg yolk replacer.

This study investigated the possibility of using amaranth protein isolate (API) as a plant-based substitute for egg yolk (EY) in the preparation of low-fat mayonnaise (LFM). The alkali extraction/acidic precipitation method was used to isolate amaranth protein; its functional properties were then studied. The results showed that besides its great water and oil absorption capacities, API had better emulsifying capacity and significantly higher (p < .05) emulsion stability at pH 2.0 than alkali pH values. Five mayonnaise samples with different API/EY combination ratios (%) (i.e., 0/0.75, 0.25/0.5, 0.375/0.375, 0.5/0.25, and 0.75/0) were prepared. The color, emulsion stability (ES), freeze-thaw stability (FTS), droplet size, structure, rheology, and sensory properties of samples were examined. API replacement showed no adverse effects on the L* value, ES, and sensory attributes (p > .05). Low API concentrations (0.25% and 0.375%) significantly (p < .05) increased the droplet size and decreased the FTS of LFM emulsion. High API concentrations (0.5% and 0.75%) had no significant effect (p > .05) on droplet size and formed emulsions with more tightly packed oil droplets. The Cross model was chosen best to describe the flow behavior of LFM samples (R 2 = 0.99). The sample with 0.75% API had significantly (p < .05) the highest values of η o (zero-shear viscosity) and λ (relaxation time), indicating greater interaction between the emulsion particles. All samples showed a weak gel structure (G' > G"). In conclusion, API can be considered an appropriate substitute for EY in LFM production, which can benefit human health and offer a new strategy for preparing vegan products.

Green Synthesis of Gold Nanoparticles Using Peach Extract Incorporated in Graphene for the Electrochemical Determination of Antioxidant Butylated Hydroxyanisole in Food Matrices.

Butylated hydroxyanisole (BHA) is a synthetic phenolic antioxidant widely used in various food matrices to prevent oxidative rancidity. However, its presence has been associated with liver damage and carcinogenesis in animals. Thus, an electrochemical sensor was built using a composite of gold nanoparticles synthesized in peach extract (Prunus persica (L.) Batsch) and graphene. Peach extract served as a reducing and stabilizing agent for gold nanoparticles, as a dispersing agent for graphene, and as a film former to immobilize the composite on the surface of a glassy carbon electrode. The gold nanoparticles were characterized using spectroscopic and microscopic techniques, and the electrodes were electrochemically characterized using electrochemical impedance spectroscopy and cyclic voltammetry. The sensor provided higher current responses and lower charge transfer resistances compared to the unmodified glassy carbon electrode. Under the established optimized working conditions (0.1 mol L-1 Britton-Robinson buffer, pH 4.0, and differential pulse voltammetry), the calibration curve exhibited a linear range from 0.2 to 9.8 µmol L-1, with a detection limit of 70 nmol L-1. The proposed sensor represented a sensitive and practical analytical tool for the accurate determination of BHA in mayonnaise samples.

Antioxidant and antimicrobial efficacy of microencapsulated mustard essential oil against Escherichia coli and Salmonella Enteritidis in mayonnaise

The aim of this study was to investigate the effect of pure and encapsulated mustard essential oil (MEO) on the shelf life of mayonnaise and its ability to be an alternative for synthetic preservatives. Determination of the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) indicated higher sensitivity of Escherichia coli O157:H7 (E. coli O157:H7) (MIC: 512 ppm, MBC: 1024 ppm) than Salmonella Enteritidis (S. enteritidis) (MIC: 1024 ppm, MBC: 2048 ppm) to MEO. Mayonnaise samples, were subsequently prepared according to the determined MIC and MBC of MEO for microbial analysis and physicochemical analysis. The antimicrobial activity of MEO in mayonnaise over 40-day storage indicated that the application of free and encapsulated MEO could inhibit the growth of target bacteria. In addition, the oxidative stability of mayonnaise samples exhibited decreasing trends over the storage time. At the end of the storage, the control sample without any preservatives revealed the highest peroxide value (3.59 meq O2 /kg of oil) whereas the sample containing 4096 ppm encapsulated MEO (2 meq O2/kg of oil) exhibited better oxidative stability, following t-butyl-hydroxyquinone (TBHQ) (1.84 meq O2 /kg of oil) as commercial antioxidant. Interestingly, the application of 2048 and 4096 ppm encapsulated essential oil had no undesirable effect on overall acceptance of mayonnaise, while the application of pure MEO at the same concentrations negatively affected the color, odor, taste and overall acceptability.

The Influence of Virgin Red Palm Oil on The Physical and Textural Properties of Various Vegetable Full-Fat Mayonnaise

Mayonnaise is a type of semi-solid oil in water emulsion containing vegetable oil as the oil phase and egg yolk (emulsifier), vinegar, salt, sugar, and spices (especially mustard) as the water phase. In this study, full-fat mayonnaise is based on various vegetable oils (sunflower oil, sesame oil, and coconut oil). Virgin red palm oil (VRPO), substituting sunflower oil, sesame oil, and coconut oil, was added to the mayonnaise formula as a 0%, 5%, 10%, and 15% bioactive component. The physical properties of the full-fat mayonnaise were investigated by measuring viscosity, pH value, color, and emulsion stability or creaming index. Textural properties including firmness, consistency, and cohesiveness were evaluated using a texture analyzer. This study aimed to identify the effect of adding VRPO to the mayonnaise formula on the physical and textural properties of the vegetable full-fat mayonnaise sample. The result of this study found that the pH value of added VRPO (5, 10, and 15%) in all mayonnaise samples was higher considerably (p&lt;0.05) compared to the mayonnaise without VRPO addition. Moreover, the color of mayonnaise from different vegetable oils when supplemented with VRPO was significantly decreased (p&lt;0.05) in the L* values, indicating a reduction in the lightness. Conversely, there was a significant increase (p&lt;0.05) in the a* values, showing an increase in redness, and b* values that demonstrated an increase in yellowness, in all of the mayonnaise samples when added with the VRPO. This study also highlighted that there was a significant difference (p&lt;0.05) in the texture between all mayonnaise samples, showing that supplemented VRPO in vegetable oils was giving a lower value of firmness and consistency and a higher value of viscosity index. In conclusion, the addition of VRPO increases the physical characteristics such as pH, color (redness and yellowness), and creaming index, as well as reduces the textural features (firmness, and consistency) in full-fat mayonnaise.

Nano formulated soy proteins as a fat replacer in low fat mayonnaise formula

It is vital to research mayonnaise egg yolk alternatives due to the greater fat and cholesterol content in egg yolks. The use of soy protein isolate and nano soy isolate as fat substitutes in mayonnaise was investigated. Two formulas were prepared, including a control (market and lab), low-fat with 0.5, 1, and 1.5% soy protein isolate (SPI) and nano soy protein isolate (NSPI), and low-fat with 0.5, 1, and 1.5% glycinin (GLY) and nano glycinin (NGLY). Rheology, microstructure, color, and sensory properties of formulas were analyzed. In comparison to the commercial and control samples, the oil droplets became smaller and more compact in the 0.5% and 1% NSPI and NGLY formulas. NSPI and NGLY bind more water and oil in the matrix of mayonnaise samples, which in turn increases the viscosity of the fluid. Furthermore, increasing the protein level to 1.5% reduced the lightness value of NSPI's low-fat mayonnaise. Mayonnaise prepared with the addition of 1% and 1.5% NSPI particles showed a higher whitening index value than other samples. Also, the samples with the highest scores for sensory attributes were the 0.5% NSPI and GLY mayonnaise samples, which did not significantly change after the end of the storage period compared to other samples. Therefore, nano soy protein and nano glycinin have potential as fat substitutes and improve the quality characteristics of low-fat mayonnaise.

Antioxidant efficiency and oxidizability of mayonnaise by oximetry and isothermal calorimetry

This study aimed to introduce a new method based on isothermal calorimetry (IC) for measuring the autoxidation rate in mayonnaise samples. Mayonnaise samples were prepared by homogenizing an aqueous phase, consisting of vinegar and egg yolk, with various oil phases, including sunflower, corn, extra virgin olive, grape seed, and apple seed oils at 60 °C. The rate of free radical formation (Ri) was controlled by adding AIBN (Ri = 4.4±0.1×10−9 M/s). The autoxidation rate determined by IC was highly correlated with the one measured using the oxygen uptake method (R2 = 0.99). The IC method accurately indicated the antioxidant capacity and rates of both inhibited and uninhibited periods, together with the oxidizability of mayonnaise samples. The mayonnaise made with extra virgin olive oil exhibited the lowest oxidizability, while sunflower oil showed maximum antioxidant efficiency. A significant advantage of the IC method was its ability to simultaneously measure up to 24 samples with minimal effort.

The Effect of Adding Pumpkin Seed Oil on Physicochemical and Sensory Properties of the Mayonnaise

The current study aimed to add pumpkin seed oil as an alternative of fat in mayonnaise without any effectiveness on the physicochemical and organoleptic properties for those product. The oil extracted from local (Iraqi) pumpkin seeds using the Soxhlet apparatus was 50%. Some chemical and physical analysis were performed. The physical analysis was done such as refractive index, viscosity, relative density, and pH at 25°C, the results were 1.48, 41c, 0.79 and 7.23, respectively. The chemical analysis such as peroxide value and free fatty acids were examined as 1.70 and 0.13, respectively. The vitamins such as E, A and D3 using (HPLC) were examined at rates 28.08, 13.17, and 1.38%, respectively. Phenols also were detected using (GC), the highest result for phenol was Vanillin (13.07%) followed by Vanillic acid (11.69) and p-coumaric acid (12.45), respectively. Whereas the fatty acids like palmitic, stearic, oleic, linoleic, linolenic, formic, lactic, acetic, propionic and butyric acids were detected in the extracted oil, they have different percentages, and the highest was 38.10% linoleic acid followed by 22.02% Oleic acid and 16.45% palmitic acid. Then, the physicochemical analysis has been done for two samples of mayonnaise made from pumpkin seeds oil and sunflower oil sample for 1, 2 and 4 weeks at a temperature (25 °C). The physical analysis was detected such as pH, solids percentage, refractive index, thermal stability of the emulsion and microscopic structure. Meanwhile, the chemical analysis also was detected such as, thiobarbituric acid (TBA), peroxide value and free fatty acids. Finally, sensory evaluation was carried out for two samples of mayonnaise made from pumpkin seeds oil and sunflower oil. The results showed that the pumpkin seed oil could improve the sensory properties of mayonnaise. Thus, this study was aimed to find healthy alternative fats that have nutritional benefits as well as to manufacture food products such as mayonnaise which was rich in antioxidants could enhances human health nutritionally and shelf life.

Physico-Chemical Characteristics, Sensory Attributes and Oxidative Stability of Soy Milk Mayonnaise Enriched in Carotenoids from Tomato By-Products

In the present study, sunflower oil was used as the extracting solvent for carotenoids and other lipophylic compounds from tomato by-products at 2.5% and 5.0% (w/v) and the carotenoid-enriched oils were further used in the manufacturing of soy milk mayonnaise. An addition of basil essential oil at 0.05% (v/v) in the carotenoid-enriched mayonnaise was also investigated. Color parameters, pH, acid, and peroxide values, as well as lipid oxidation as measured by TBARS values were monitored in control and in supplemented mayonnaise samples during refrigerated storage for up to 8 weeks. The effect of enrichment on the sensory attributes of mayonnaises was also studied. The extraction of dry tomato by-products resulted in a significant increase in the total carotenoid content and antioxidant activity of the oils while their color changed significantly by increasing the redness and decreasing the lightness and yellowness. The use of carotenoid-enriched oils in the mayonnaise manufacture increased the oxidative stability of soy milk mayonnaise during storage and improved the flavor and the chromatic characteristics of mayonnaise compared to the control sample without significantly affecting its consistency and overall acceptability. The addition of basil essential oil (0.05% v/v) enhanced the oxidative stability and improved the sensory profile of the mayonnaise.

The garlic extract-loaded nanoemulsion: Study of physicochemical, rheological, and antimicrobial properties and its application in mayonnaise.

In this research, garlic extract (GE)-loaded water-in-oil nanoemulsion was used as a novel preservative and antioxidant in mayonnaise. GE (5%, 10%, 15%, and 25%) as a dispersed phase, olive oil as a continuous phase, and polyglycerol polyricinoleate (PGPR) as a low HLB surfactant, with a constant surfactant/garlic extract ratio (1:1), were used in the formulations of water-in-oil nanoemulsions. The properties of the active nanoemulsion, including droplet size, free radical scavenging capacity, antimicrobial activity against gram-positive (Staphylococcus aureus [25923 ATCC]), and gram-negative (Escherichia coli H7 O157 [700728 ATCC]) were evaluated. The results showed that the mean droplet size of nanoemulsion increased from 62 to 302 nm and antioxidant capacity was also improved from 95.43% to 98.25% by increasing GE level from 5% to 25%. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) showed that antimicrobial activity against S. aureus could be observed only in high levels of GE (25%) in the formulation of nanoemulsion. The results of the total count analysis showed that the GE-loaded nanoemulsion (NEGE) was effective against the microorganisms, particularly after 4 months of storage. The incorporation of GE and NEGE did not affect significantly the acidity of different mayonnaise samples; however, they affected the concentration of the primary product of lipid oxidation. Adding GE and NGE did not significantly affect the rheological properties of mayonnaise and all samples showed shear-thinning behavior. Sensory evaluation showed that the samples with NEGE had higher scores in texture, spreadability, and mouthfeel, while the control samples had better scores in appearance, color, taste, and total acceptance. In general, the samples containing free GE (not encapsulated) had the lowest scores in all organoleptic characteristics.

Formulation of functional mayonnaise with defatted wheat germ flour as egg yolk substitute: Rheological, textural and stability analyses

The aim of this research was to study the feasibility of the defatted wheat germ flour (DWGF) alone or in combination with xanthan gum (XG) as egg yolk substitutes in mayonnaise formulation. In this research, DWGF was used at different levels (0, 25, 75 and 100%) alone or in combination with the XG (0.2%) as egg substitutes. The effects of these substitutions on the stability, textural and rheological characteristics of mayonnaise were investigated. According to the results, DWGF can be used as an egg yolk substitute in mayonnaise. Combining DWGF and XG as egg yolk substitute, improved the stability, textural and rheological properties. Mayonnaise with 100% egg yolk replacement showed the hardest structure, while the lowest firmness value was obtained in the control containing 100% egg yolk. Also, the highest adhesiveness value occurred in the sample with 100% DWGF. The thixotropic behavior was observed for all investigated mayonnaise samples over the all ranges of the studied shear rate (0.05-450/sec). Furthermore, the highest content of consistency coefficient value was observed in the sample with 100% DWGF and 0.2% XG. Therefore, it can be concluded that low-cholesterol mayonnaise with proper stability, textural and rheological characteristics are achievable by applying of the desirable combination of DWGF and XG.

Effect of Vinegar on the Oxidative Stability of Mayonnaise During its Storage.

Mayonnaise is an emulsion of oil that is susceptible to lipid oxidation, which can cause spoilage and the formation of harmful compounds. This study aims to evaluate the impact of Syrian apple and grape vinegar on the oxidative stability of mayonnaise and compare the use of natural antioxidants to synthetic ones such as butylated hydroxyanisole and butylated hydroxytoluene. The study measured total phenol content, radical scavenging activity, and identified some phenolic compounds by High Performance Liquid Chromatography (HPLC). The rancidity of mayonnaise was examined using peroxide value and thiobarbituric acid number. The fatty acids content in the mayonnaise samples was examined using gas chromatography. Vinegar samples containing high concentrations of phenolic antioxidants showed high ability to scavenge free radicals. The antioxidants in vinegar protected the mayonnaise samples from primary and secondary oxidation, and there was no statistically significant difference between the ratio of unsaturated fatty acids in the samples containing vinegar at the beginning and at the end of the storage period. The study shows the significance of using vinegar to protect mayonnaise from deterioration and increase its shelf life, in addition to its role as a dressing.

Effect of Black Cumin Cake Extract, Octyl Caffeate, and Active Packaging on Antioxidant Properties of Egg-Free Mayonnaise during Storage

The egg-less mayonnaise (CM) based on aquafaba and refined rapeseed oil was prepared and enriched with black cumin cake extract (BCCE) at two concentrations of 0.1% (BCCM0.1%) and 3% (BCCM3%) and 0.1% of octyl caffeate (OCM). The obtained mayonnaises were placed in glass jars without and with active film incorporating BCCE at the jar bottom (AFM). The influence of antioxidants on oxidative stability and antioxidant capacity (AC) of the prepared mayonnaises was estimated by the shelf-life test. Peroxide value (PV), anisidine value (AnV), total oxidation (TOTOX) value, acid value (AV), and amounts of conjugated dienes (CD) and conjugated trienes (CT) were used to assess the extent of mayonnaise deterioration during storage of up to 4 weeks in a refrigerator. The synthesized octyl caffeate (OC) and natural antioxidants from BCCE added to mayonnaises directly and released from active film enhanced the AC of the studied mayonnaise samples determined using the QUick, Easy, New, CHEap, and Reproducible-2,2-diphenyl-1-picrylhydrazyl (QUENCHER-DPPH), and QUENCHER-2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (QUENCHER-ABTS) methods. All enriched mayonnaises had higher antioxidant potential than the AC of the control sample without antioxidants (CM) and commercial plant-based mayonnaise (CPBM). Therefore, the proposed antioxidants and active film can be used in the food industry to suppress lipid oxidation.

Characteristics of reduced-fat mayonnaise prepared by oleaster as a fat replacer and natural antioxidant.

Due to the disadvantages of consuming fat and synthetic preservatives, the demand to reduce them in lipid-based products like mayonnaise is increasing. In the current research, there were two goals, the first one was studying the effect of using oleaster flour in different concentrations (4%, 6%, and 8%) as a natural preservative, whereas the second one was studying the effect of oleaster as different fat replacement (FR) levels (10%, 20%, 30%, and 40%) on the physicochemical, antioxidant, and the rheological properties and stability of reduced-fat mayonnaise samples. Given results showed that with increasing the oleaster concentration, the antioxidant property increased significantly. The peroxide value after 60 days of storage for the 30% FR 8 was 2.01%, compared to the control sample without antioxidant and with TBHQ, which were 10% and 2.68%, respectively. The highest stability index (100%) was observed in the 30% FR and 40% FR samples. In terms of rheological characteristics, the 30% FR 8 oleaster showed the highest viscosity and the lowest frequency dependency. It can be concluded that oleaster has a high potential to be used in the formulation of low-fat mayonnaise as a fat replacer.

Investigating the physical and quality characteristics and rheology of mayonnaise containing aquafaba as an egg substitute

This paper aimed to examine the effect of using aquafaba in different ratios (25%, 50%, 75%, 100%) as an egg yolk substitute on the physical, structural, and rheological properties of mayonnaise. Large amplitude oscillatory shear analysis (LAOS) parameters (ηL′, ηM′, G'L, G'M, e3, e1, v3, v1) were evaluated to determine the rheological properties of mayonnaise samples. The droplet size of samples significantly increased and the stability of the emulsion decreased after 50% substitution ratio. The density of mayonnaise significantly increased with increased aquafaba substitution. According to Lissajous curves, egg yolk substitution with aquafaba leads to a decrease in stress values. Aquafaba affected the microstructure of samples and decreased the dissipated and stored energies. The LAOS parameters showed that the sample's behavior is strain stiffening and shear thinning in the nonlinear region. Textural parameters decreased with increased ratio of aquafaba. Significant differences were observed for L*, a*, b* and ΔE values.

Assessment of Bioactive Phytochemicals and Utilization of Rosa canina Fruit Extract as a Novel Natural Antioxidant for Mayonnaise.

The oxidation of food emulsions causes rancidity, which reduces their shelf life. To prevent rancidity, synthetic antioxidants are widely used in the food industry. However, due to their potential health risks, researchers are exploring natural alternatives. This study aimed to investigate whether Rosa canina fruit extract (RCFE) could be used as a natural antioxidant to extend the shelf life of mayonnaise. Mayonnaise containing varying concentrations of RCFE [0.125% (T1), 0.25% (T2), 0.50% (T3), 0.75% (T4)] was compared to a mayonnaise control sample (C1) and a mayonnaise sample containing 0.02% BHT (C2) for 60 days of storage at 4 °C. RCFE was found to have high levels of total phenols content (52.06 ± 1.14 mg GAE g-1), total flavonoids content (26.31 ± 1.03 mg QE g-1), and free radical scavenging activity. The GC-MS analysis of RCFE revealed 39 different peaks, whereas the HPLC analysis showed the presence of 13 polyphenolic compounds in RCFE. The pH values of T2, T3, and T4 mayonnaise samples substantially declined as storage progressed; however, the reduction was less than that of C1 and C2. After 60 days, mayonnaise samples T2, T3, and T4 had greatly reduced peroxide and free fatty acid levels compared to C1 and C2. The mayonnaise enriched with RCFE (T3 and T4) had the most potent antioxidative ability and the lowest value of lipid hydroperoxides (peroxide value, POV) and the lowest value of thiobarbituric-acid-reactive substances (TBARS). The sensory evaluation revealed that the T3 sample exhibited the highest overall acceptability. In conclusion, this study recommends that RCFE could be used as a natural preservative to enhance the shelf life of functional foods.

ANÁLISE DO NÍVEL DE CONTAMINAÇÃO DE MAIONESES ARTESANAIS NO CRATO – CE E JUAZEIRO DO NORTE – CE

Mayonnaise is an attraction in snack bars. Due to its nutritious base, it forms a perfect environment for the growth of microorganisms. The objective is to verify the presence of microorganisms present in the collected samples and quantify them through the use of the EMB and SS culture media. This is an experimental work where 7 samples of artisan mayonnaise were collected, being 2 samples produced by popular snack bars in Crato - CE and 5 in Juazeiro do Norte - CE. After identification, 100 g of the samples were fractionated from the seven establishments, packed in sterile bags and transported in an isothermal box with ice, and preserved until the moment of the analysis. For culture, mat culture was performed on Eosin Methylene Blue Agar (EMB) and Salmonella Shigella-SS Agar. After multiplying the dilution factors, the number of CFU/ml in the samples was obtained.&nbsp; In the ones from Crato the growth of colonies occurred in the SS concentrate and in its 10:1 dilution, in the other dilutions we verified that there was only the presence of colorless streaks indicating Shigella sp, in the EMB seeding there were only streaks with a metallic green aspect being indicative of Escherichia coli. In Juazeiro, we obtained growth in all samples in the SS and EMB media, with colonies indicating Salmonella sp. and Enterobacteriaceae. We conclude that the storage or production process shows signs of contamination by pathogens, putting people at risk. It is necessary to seek effective means of sanitation capable of eliminating the presence of pathogens in the food production process. Keywords: Feeding; Contamination; Salmonella sp

Physio-Chemical Properties and Oxidative Stability of Microencapsulated Silkworm Pupae Oil Enriched Mayonnaise

This study investigates to enrich the mayonnaise with novel oil replacer which is from silkworm pupae insect. Due to their health benefits, the well-known omega-3 and omega-6 fatty acid families are the most significant polyunsaturated fatty acids (PUFA) demanded in foods. Insects are traditionally consumed as food in different parts of the world since they are both a good source of protein and rich in essential fatty acids (EPA and DHA). The product Mayonnaise was developed using Encapsulated Silkworm Pupae Oil powder (Mayo-ESOP of 10 g) and Silkworm Pupae Oil (Mayo-SPO OF 10 ML) and was compared with control mayonnaise prepared out of sunflower oil. The mayonnaise samples were analysed for oxidative stability through peroxide values at 0, 7, 14 and day 21 days and after preparation of physical parameters such as droplet size for surface (µm), volume (µm), viscosity (γ=10 s-1), proximate analysis, colour values (*L, *a and *b) fatty acids profile. The study revealed that Mayo-ESOP and Mayo-SPO samples showed better oxidative stability as a result of this not affecting any quality aspects of mayonnaise samples. The proximate composition among the three variations of mayonnaise Mayo-SPO was found to have a maximum fat content of 76.13±0.01g per 100 g, followed by Mayo-ESOP 72.3±0.01g/100 g and Control 66.21±0.01g/100 g respectively. The fat composition indicates that silkworm pupae have a very good fatty acid profile, which includes omega 9 and omega 6. The colour values (L*, a*, b*) and the total colour difference represented as (ΔE values) had no much significant difference show the yellowness colour for mayonnaise sample compared to control due to the presence of carotenoids. Overall, the colour assessment reveals that the oil was of good quality. Oxidative stability indicates the mayonnaise samples stored at a refrigerated temperature for 0, 7, 14, and 21 days had significantly difference on peroxide values due to oxidation of mayonnaise samples.

The stabilisation of low‐fat mayonnaise by whey protein isolate‐microbial exopolysaccharides (Weissella confusa W‐16 strain) complex

SummaryThis study aims to evaluate the fat‐substituting feature of the complex of whey protein isolate (WPI) and dextran structure exopolysaccharide (EPS) synthesised by Weissella confusa JCM 1093 strains in mayonnaise samples. The flow behaviour, frequency sweep and three interval thixotropy test properties of the samples were compared with the control groups. Thermal loop test was applied to determine the emulsion stability of the samples. Oxidative stability was tested with OXITEST, and induction period values were compared. K values of the samples varied between 20.05 and 169.13 Pa.sn and the n values vary between 0.133 and 0.383. Samples showed pseudoplastic behaviour (n &lt; 1). G' of all samples is greater than G", meaning that all samples showed a solid‐like behaviour like conventional mayonnaise. The complex of 2% EPS and 5% WPI provided a solid structure and improved the physical stability of low‐fat mayonnaise. The results showed that the samples had high physical stability when they experienced thermal stress at low and high temperatures. As a result, the WPI and EPS complex can be used as a fat replacer for improving the rheological properties, emulsion and oxidative stability in low‐fat mayonnaise samples.