Ephemeroptera Research Articles

River invertebrate biodiversity benefits from upstream urban woodland

In urban environments, invertebrate communities are subjected to a broad mixture of impacts, including diffuse pollution. Pollutant mixtures and habitat degradation can combine to apply stress on community diversity. Water quality is influenced by the assemblage and mosaic of catchment land cover. Amongst a wider suite of Nature-Based Solutions, the value of urban woodland is increasingly recognized as having potential to support a range of ecosystem services. Despite an increasing focus on establishing urban woodland for aquatic conservation, its actual influence is yet to be manifested. Therefore, we explored trees’ location in riparian and upstream catchment, within and outside of the urban area. We conducted a combination of systematic literature review and statistical analysis to better understand the woodland influence. Despite the wide range of bioindicators studied and broad worldwide spectrum of geo-climatic regimes covered, literature evidence for benefits were found in at least half the cases. With a focus on the overall family richness and the sensitive orders Ephemeroptera, Plecoptera and Trichoptera family richness as bioindicators, the statistical analysis comprised a national study in England covering 143 sites with substantial urban cover, totaling 4226 invertebrate community observations over 30 years. Two satellite-derived land cover maps were used to enable discrimination between urban and extra-urban woodland. The analysis supported the literature evidence that impervious land had negative effects and woodland positive effects. In the urban and upstream catchment, woodland was more important than pasture or cropland. There was some evidence of those woodland effects being more advantageous when trees are located within the urban area itself. Benefits attributable to woodland were distinctly apparent against a backdrop of improving macroinvertebrate diversity found to be synchronous with long-term reductions in urban pollution signatures. The presence of sparse land, even in small amounts, was detrimental to macroinvertebrate diversity. These areas of low vegetative cover might be detrimental due to high sediment input and legacy industrial contamination. Given the increasing accessibility of land cover data, the approach adopted in this case study is applicable elsewhere wherever macroinvertebrate community data are also available.

Benthic invertebrate fauna of a forest spring on the Muravyov- Amursky Peninsula (Primorsky Krai, Southern Russian Far East)

The fi rst data on spring macroinvertebrate fauna of the Southern Russian Far East are presented. Macrobenthos of a small forest spring classifi ed as helocrene includes at least 21 species from 20 genera and 17 families in Arthropoda and Platyhelminthes. Main groups of benthic organisms are amphipods, planarians, larvae of dipterous insects, caddisfl ies, stonefl ies and mayfl ies, small water beetles. The following invertebrates are defi ned to the species level: planaria Phagocata sibirica, stonefl y Skwala compacta, caddisfl y Glossosoma angarensis, aquatic beetles Hydraena riparia (imago) and Heterlimnius gapyeongensis (larvae), amphipods Gammarus koreanus and hypogean Procrangonyx primoryensis. The macrobenthos of the spring is also ecologically diverse. Here, rheo- and stagnophiles, highly specialized stygo- and crenobionts, and species with wide ecological niches, sharply diff ering in their preferences, closely coexist.

Mayfly optimization algorithm: a review

This paper gives a review on the bio-inspired optimization methodology known as mayfly (MA) algorithm in order to resolve issues in optimization techniques. It is a newly formed meta-heuristic optimization algorithm that focuses on the movements of masculine and feminine mayflies. It is encouraged from flying behaviour also the methods of mating in mayflies. With the help of a realistic-world separate flow planning issue along with the coupling behaviour in numerous objective optimizations, the performance of the mayfly algorithm (MA) is well evaluated. Some of the implementations of this algorithm are discussed in this paper: Bearing fault diagnosis based on the mayfly algorithm, optimizing the performance of PEMFC, Covid diagnosis, wind speed optimization, improving the scheduling of solar wind speed using mayfly optimization, detecting fault in the wind turbine gearboxes, patterning in the array antennas with the help of optimization and so on .One of the main advantages of the MA is that it combines the other optimization algorithms namely swarm optimization (PSO) with the evolutionary optimizations (GA). The motion of the mayflies that resemble nuptial dance model along with the arbitrary flight helps in the improvement of the stability within the exploration and exploitation methods. In addition, allows escape from the community peak. All the above work reviewed shows promising results from the algorithm. More work can be carried out using this algorithm in future.

A comprehensive DNA barcoding reference database for Plecoptera of Switzerland

DNA barcoding is an essential tool in modern biodiversity sciences. Despite considerable work to barcode the tree of life, many groups, including insects, remain partially or totally unreferenced, preventing barcoding from reaching its full potential. Aquatic insects, especially the three orders Ephemeroptera, Plecoptera, and Trichoptera (EPT), are key freshwater quality indicators worldwide. Among them, Plecoptera (stoneflies), which are among the most sensitive aquatic insects to habitat modification, play a central role in river monitoring surveys. Here, we present an update of the Plecoptera reference database for (meta)barcoding in Switzerland, now covering all 118 species known from this country. Fresh specimens, mostly from rare or localized species, were collected, and 151 new CO1 barcodes were generated. These were merged with the 422 previously published sequences, resulting in a dataset of 573 barcoded specimens. Our CO1 dataset was delimited in 115 CO1 clusters based on a priori morphological identifications, of which 17% are newly reported for Switzerland, and 4% are newly reported globally. Among the 115 CO1 clusters, 85% showed complete congruence with morphology. Distance-based analysis indicated local barcoding gaps in 97% of the CO1 clusters. This study significantly improves the Swiss reference database for stoneflies, enhancing future species identification accuracy and biodiversity monitoring. Additionally, this work reveals cryptic diversity and incongruence between morphology and barcodes, both presenting valuable opportunities for future integrative taxonomic studies. Voucher specimens, DNA extractions and reference barcodes are available for future developments, including metabarcoding and environmental DNA surveys.

Diversity of Freshwater Macroinvertebrate Communities in Los Tuxtlas, Veracruz, Mexico

The objective of this work is to contribute to the knowledge of the freshwater macroinvertebrate communities of Los Tuxtlas, Veracruz, Mexico. For this region, there is only limited knowledge of its aquatic crustaceans and mollusks. A total of 13,399 freshwater macroinvertebrates were collected from four river sections in each of the three sub-basins of the region using the Surber network in four seasons of an annual cycle (2021–2022) and were preserved in 70° alcohol. Organisms belonging to seven phyla, nine (sub)classes, 21 (sub)orders and 65 families were identified. The most abundant orders were Ephemeroptera (42.03%), with greatest abundance of the family Baetidae, and the orders Trichoptera (19.11%), Diptera (15.43%), and Coleoptera (3.98%). Four families exceeded 10% relative abundance, and together they total 61.02%: Baetidae (23.84%), Hydroptilidae (13.58%), Leptohyphidae (13.03%), Chironomidae (10.57%), and Elmidae (3.23%). The order Plecoptera was recorded for the first time in Los Tuxtlas, with three families. The orders Hydrachnidae and Ostracoda, as well as six families of the order Ephemeroptera, with only one previously recorded family, and six more families of the order Diptera, were also documented. Two species of invasive aquatic mollusks were found in several rivers and basins. In this work, a high diversity of freshwater macroinvertebrates occurred compared to other sites studied in Veracruz and Mexico, and new records of these taxa are provided for the region of Los Tuxtlas.

The thermal breadth of temperate and tropical freshwater insects supports the climate variability hypothesis.

Climate change involves increases in mean temperature and changes in temperature variability at multiple temporal scales but research rarely considers these temporal scales. The climate variability hypothesis (CVH) provides a conceptual framework for exploring the potential effects of annual scale thermal variability across climatic zones. The CVH predicts ectotherms in temperate regions tolerate a wider range of temperatures than those in tropical regions in response to greater annual variability in temperate regions. However, various other aspects of thermal regimes (e.g. diel variability), organisms' size and taxonomic identity are also hypothesised to influence thermal tolerance. Indeed, high temperatures in the tropics have been proposed as constraining organisms' ability to tolerate a wide range of temperatures, implying that high annual maximum temperatures would be associated with tolerating a narrow range of temperatures. We measured thermal regimes and critical thermal limits (CTmax and CTmin) of freshwater insects in the orders Ephemeroptera (mayflies), Plecoptera (stoneflies) and Trichoptera (caddisflies) along elevation gradients in streams in temperate and tropical regions of eastern Australia and tested the CVH by determining which variables were most correlated with thermal breadth (T br = CTmax - CTmin). Consistent with the CVH, T br tended to increase with increasing annual temperature range. T br also increased with body size and T br was generally wider in Plecoptera than in Ephemeroptera or Trichoptera. We also find some support for a related hypothesis, the climate extreme hypothesis (CEH), particularly for predicting upper thermal limits. We found no evidence that higher annual maximum temperature constrained individuals' abilities to tolerate a wide range of temperatures. The support for the CVH we document suggests that temperate organisms may be able to tolerate wider ranges of temperatures than tropical organisms. There is an urgent need to investigate other aspects of thermal regimes, such as diel temperature cycling and minimum temperature.

Effects of Anthropogenic Activities on Physicochemical Characteristics and Macroinvertebrate Distribution in Riverine System of Misamis Occidental

Rivers are exposed to high human pressures with apparent effects on riparian organisms and other biological entities. This study was conducted to determine the effects of anthropogenic activities on the physicochemical characteristics and the distribution of macroinvertebrates in the riverine system of Misamis Occidental. Standard methods were followed to measure water temperature, water pH, and relative humidity. Macroinvertebrates were sampled using a handheld dip net and a one-meter kick net. Results showed that human activities were evident in all sampling areas. A total of 8 orders with nine families of macroinvertebrates were recorded. Among the macroinvertebrates, the Leptophyphidae family of the order Ephemeroptera (Mayflies), a good indicator of water quality, was the most abundant. Based on the Family Biotic Index, sampling site two has excellent water quality, which means organic pollution is unlikely. The findings of this study can be utilized as a basis for developing a protection and management plan for the river. Keywords : Coleoptera, dip net, Ephemeroptera, family biotic index, temperature

Relationships between Land Use Changes and Benthic Macroinvertebrates’ Community Structure in an East African Riverine Environment

Anthropogenic land use activities affects the health status of river ecosystems in supporting aquatic biodiversity. Undisturbed wetlands ensure water bodies are ecologically healthy for community livelihoods. Recent years have witnessed degradation of aquatic ecosystems and this has devastating effects on the environment and community livelihoods. One of such wetlands is river Isiukhu of western Kenya. This study analysed water physico-chemical parameters and benthic macroinvertebrates’ assemblages of River Isiukhu between August to November, 2022. Four sampling stations were selected along the longitudinal river gradient based on anthropogenic impact and dominance of land use activities. Sampling stations were classified as ‘very high’, ‘high’, ‘moderate’ and ‘low’ and rated 4,3,2 and 1 respectively, depending on the anthropogenic impact. Macroinvertebrates were sampled using D-frame net and classified using appropriate keys. Anthropogenic activities and habitat characteristics were noted and recorded. Water quality parameters were determined in situ. Macroinvertebrates’ population indices were calculated to determine community structure. Sorenson’s Coefficient quantified similarities of species in different stations. Pearson correlation and ANOVA tested the study hypothesis. About 1,391 invertebrates were collected belonging to 12 orders, 32 families and 30 genera. Orders Ephemeroptera, Hemiptera, Diptera and Coleoptera recorded highest percentage taxonomical composition. Upper pristine stations (F and G) supported more species richness and diversity than lower stations (L and T). Macroinvertebrates’ communities in terms of diversity, abundance, richness, evenness, and functional feeding groups responded differently to impacts of anthropogenic activities, due to their varied adaptation traits. Water quality varied significantly between sampling stations (ρ <0.05). Close relationship existed between land use activities and species diversity (r=0.8; ρ < 0.05); richness (r=0.8779; ρ < 0.05); evenness (r=0.1067; ρ < 0.05); and abundance (r=0.962; ρ < 0.05). The study recommends the need to conserve riparian vegetation as a way of mitigating and adapting to the impact climate change on stream ecosystem.

Altitudinal and seasonal distribution of benthic macroinvertebrates in River Tons - a tributary of Yamuna River, Uttarakhand, India.

The main tributary of the Yamuna, the Tons River, exhibits altitudinal changes in its macroinvertebrate community's diversity, abundance, and composition. Between May 2019 and April 2021, the study was conducted in the upper section of the river. A total of48 numbers of taxa from 34 families and ten orders were recordedduring the investigation. At this elevation of 1150 to 1287m, the two most predominant orders are Ephemeroptera (32.9%) and Trichoptera (29.5%). During the premonsoon season, they had the lowest macroinvertebrate density (250-290 individuals/m2), and the post-monsoon season had the highest density (600-640 individuals/m2). During the post-monsoon season, the maximum larval forms (60%) of various insect orders were predominant. The findings indicated that lower altitudes (1150-1232m) have higher macroinvertebrate abundance than higher ones. The diversity of dominance is shallow at site-I (0.0738) and strong at the site-IV during the premonsoon season (0.03837). Taxa richness, as measured by the Margalef index (D), peaked in the spring season (January to March) at 6.9 and reached its lowest point (5.74) in the premonsoon season (April to May). Only 16 taxa were discovered in site-I and site-II, but 39 taxa were discovered at low altitudes (site-IV, 1100m) (1277-1287m). The Tons River contains a total of 12 and 13 genera, respectively, that belong to the orders Ephemeroptera and Trichoptera, according to qualitative study of the macroinvertebrates. The current study supports the use of macroinvertebrates as bioindicator species for monitoring biodiversity and assessing the health of ecosystems.

Two new species of Baetodes Needham & Murphy, 1924 with an updated checklist of Baetidae (Ephemeroptera) of Rondônia state, Brazil.

Baetidae is one of the most taxon-rich families in the order Ephemeroptera, and the most studied mayfly in Brazil. Despite recent advances, the vast majority of the Brazilian territory has not yet had its diversity well documented. Some of these critical gaps are in the South-Western Amazon, with emphasis on the state of Rondônia due to the high level of environmental threat (especially deforestation). In the state, only one small survey was carried out 11 years ago. Given this scenario, the objective of this study is to describe two new species and provide access to primary biodiversity data of the family Baetidae in Rondônia. As a result, two new species, Baetodes vermelho sp. nov. and Baetodes cerejeiras sp. nov., are described. Baetodes vermelho sp. nov. can be differentiated from other species by the presence of robust and pointed setae on the dorsal margin of the femora and coxal gills with medial constriction; Baetodes cerejeiras sp. nov. can be differentiated from B. liviae Polegatto & Salles, 2008 (in Salles & Polegatto, 2008) and B. capixaba Souza, Salles & Nessimian, 2011 by the absence of a tubercle on the metanotum, the presence of small blunt setae on the dorsal arc of the labrum, and less prominent tubercles on the abdomen. We have documented 50 species across 54 sites in the Rondônia. Among these, 18 species are being reported for the first time in the state, three species are recorded for the first time in the north of Brazil, and two species are new records for Brazil. Rondônia is the most speciose state in Brazil regarding the family Baetidae.

MATERIALS ON THE FAUNA OF EPHEMEROPTERA, PLECOPTERA AND TRICHOPTERA OF THE KRONOTSKY NATURE RESERVE AND OTHER REGIONS OF KAMCHATKA

As a result of collection in 2014–2021, additional information was obtained on the fauna and distribution of three orders of amphibiotic insects Ephemeroptera, Plecoptera and Trichoptera of the Kronotsky State Natural Biosphere Reserve and other regions of the Kamchatka Peninsula. The general list of the three orders the studied territory includes 56 species: 8 species of mayfl ies, 17 – stonefl ies, and 31 – caddisfl ies. For the territory of Kronotsky Nature Reserve are noted 50 species: 8 – mayfl ies, 11 – stonefl ies, and 31 – caddisfl ies; the presence of 48 previously known species is confi rmed. Stonefl y Capnia nigra (Pictet, 1833) and caddisfl y Hydropsyche kozhantschikovi are fi rst fi nds for the reserve. Plecopterans Plumiperla diversa (Frison, 1935) is discovered for Geyser Valley streams for the fi rst time, and Isocapnia orientalis Zhiltzova, 1975 is a new fi nding for the Kamchatka Peninsula.

THE MAYFLIES (INSECTA, EPHEMEROPTERA) OF THE NORTH OF THE FAR EAST OF RUSSIA

Based on the literature and original data, a review of the fauna of mayflies (Ephemeroptera) of the North of the Russian Far East is given. To date, the faunistic list of mayfl ies in the region includes 72 species from 23 genera and 8 families. In the watercourses of the Magadan Region, 52 species are recorded, Chukotka Autonomous District – 27, the north of the Khabarovsk territory (a part of Okhotsk north of the Tugur River in the south and the Okhota River in the north) – 57, and Kamchatka – 37 species. Within local faunas, species with the Eastern Palearctic type of distribution prevail in all regions of the North of the Far East, reaching a maximum in north of the Khabarovsk Territory (54 %). The species richness of mayfl ies revealed for the North of the Far East is much less than in the watercourses and reservoirs of the south of the Far East and Eastern Siberia (bass of Lake Baikal), but quite comparable with the fauna of adjacent territories and other northern European countries.

ESTIMATION OF WATER QUALITY ACCORDING TO THE EPT INDEX IN THE KAMCHATKA PENINSULA RIVERS IN AUTUMN, TAKING INTO ACCOUNT REGIONAL FEATURES OF THE FAUNA

The paper presents the results of the water quality assessing in the surveyed areas of the foothill Kamchatka Peninsula Rivers in the autumn of 2023. The EPT index of indicator groups of mayfl ies (Ephemeroptera), stonefl ies (Plecoptera), and caddisfl ies (Trichoptera) in the streams of the Bolshaya, Kamchatka, and Avacha River Basins varied within 8–16, which corresponds to the standard of good-fair or fair water quality. However, given that the fauna of these indicator orders of aquatic insects in the Kamchatka Rivers is slightly more than 20 % of that in the south of the Far East; these regional features are also refl ected in the indices when assessing water quality, in particular, in the number of EPT species. And if the water quality is considered good if there are 24–31 species in the rivers of the south of the Far East, then for the watercourses of Kamchatka such water quality, which we assessed with an index of 10 to 16, is quite justifi ed. It is also necessary to take into account seasonal changes in the fauna, since the samples were taken in September, when most of the indicator taxa left the streams, and a number of species of the new generation had not yet hatched. Our results can be confi rmed by studies conducted earlier in the areas of existing economic facilities on the Kamchatka Peninsula, which showed that the EPT index in the background river sections varied from 10 (Agi R.) to 13 (Tolmacheva R.), which is comparable with our results.

THE LONG-TERM DYNAMICS OF ZOOBENTHOS COMMUNITIES IN THE LAKE UNDER CONDITIONS OF WASTEWATER POLLUTION FROM APATITE- NEPHELINE PRODUCTION

The changes in the composition and structure of zoobenthos of Bolshoi Vudyavr Lake were analyzed in the long-term dynamics. Benthic fauna is characterized by low taxonomic diversity. Zoocenosis consists of eurybiont species of oligochaetes (Tubifi cinae), chironomids (Chironiminae, Tanypodinae), and mollusks (Pisidiidae) in the deep-water zone. Chironomids Orthocladiina dominated in the littoral zone. Communities are complemented by bivalves, oligochaetes and invertebrates typical for the littoral zone of the lakes (gastropods, caddisfl y, beetles, mayfl ies, stonefl ies). In the structure of deep-water biocenoses there is a tendency of increasing of mollusks Pisidiidae. Their share in the total number of invertebrates increased from 30 % in 2001 to 66 % in 2022. Zoobenthos are characterized by relatively high values of abundance number and biomass. The average level of biomass of zoobenthos in the lake varies from “moderate” (5,0–10,0 g/m2) to “elevated” class (10–20 g/m2).

Aydın ve Denizli (Türkiye) illeri Ephemeroptera (Insecta) faunası üzerine araştırmalar

Although studies on the order Ephemeroptera are at a sufficient level in many of the provinces in the Aegean region, there is no comprehensive study on the provinces of Aydın and Denizli. Until now, four species from Aydın and two species from Denizli province have been reported from the Ephemeroptera order. The research being done in these provinces, which are the study regions, aims to fill in the gaps in the Aegean Area and provide more precise information about the national Ephemeroptera fauna.
 With the aid of sieves and water scoops, nymphs were gathered from the habitats of each locality's various biological characteristics during the field experiments, which were conducted in 25 different locations throughout two provinces. Thirteen species were recognized after examination of the 1177 collected specimens.
 In the study, five of the species identified from Aydın province and 12 of the species identified from Denizli province were recorded for the first time from the related provinces within the research area.

KEANEKARAGAMAN JENIS DAN KELIMPAHAN ORGANISME SERANGGA DI HUTAN RAWA GAMBUT YANG DIKONVERSI MENJADI SAWAH DI PEAT TECHNO PARK UNIVERSITAS PALANGKA RAYA

This study aims to identify the species diversity and abundance of insects in the peat swamp forest converted into rice fields in the Peat Techno Park, University of Palangka Raya. The sampling method used three types of traps, namely yellow trap, swing nets,and light traps. The Shanon -Weiner equation was used to calculate the diversity index of the collected insects. The insects that were caught consisted of 10 orders and 49 families with a total 0f 2,089 individuals. The most caught insects were the order Ephemeroptera with a total 730 individuals. The abundance index of Ephemeroptera was 35.04%, Formicidae 11.20%, Coenagrionidae 10,39%, and Tetragnathidae 9,38%. The four of this insects are classified as dominant. The H’ index is 2.5037 where the level of insect diversity is moderate, the dominance index is 0.1626 so that dominance is classified as moderate. The evenness index is 0.6276, which means moderate evenness.

A Longitudinal Assessment of Benthic Macroinvertebrate Diversity and Water Quality along the Bronx River

The Bronx River is an urban waterway with a long history of anthropogenic disturbance. We conducted a longitudinal assessment of the Bronx River's water quality by measuring benthic macroinvertebrate diversity at 6 sites along the river. We integrated long-term water-quality data collected by the New York State Department of Environmental Conservation. We found that the overall water quality of the river has remained moderately impacted over different timepoints throughout the past 22 years. The study site upstream of combined sewage overflows and municipal separate stormwater systems exhibited healthier biological profiles, whereas the most-downstream sites exhibited slight declines in water quality. The most recent survey of the Bronx River (2020) revealed that high invasive species dominance was associated with benthic macroinvertebrate communities that were less healthy. Notably, one invasive species not documented in historical surveys, Corbicula fluminea (Asian Clam), was sampled in 5 of 6 study sites during the 2020 surveys. Moreover, no species were sampled from the order Ephemeroptera (mayflies) in 2020 despite being present in previous surveys. These results can be used to guide the management of urban rivers.

Complete mitochondrial genome of Macromia manchurica Asahina, 1964 (Odonata: Macromiidae)

We describe the first time sequencing and assembly of the complete mitochondrial genome of Macromia manchurica Asahina, 1964 (Odonata; Macromiidae; Macromia). The mitochondrial genome of M. manchurica was found to be 15,560 bp. It contains thirteen protein-coding genes (PCGs), 22 transfer RNAs (tRNAs), two ribosomal RNAs (rRNAs), and AT-rich region. The overall base composition of A. japonicus is A-38.6%, C-17.0%, G-12.5%, and T-31.9%. A phylogenetic analysis of 14 species within the order Odonata and order Ephemeroptera suggested that Macromia amphigena is most closely related to M. manchurica.

Makrozoobenthos As A Bioindicator Of Water Quality In The Masang Kecil River In West Pasaman Regency

Sungai Masang Kecil is located in the middle of the PT Andalas Agro Industri (PT AAI) palm oil plantation and mill. This activity will have an impact on aquatic biota, especially benthos. Benthos occupies an important position in the food chain and lives permanently on the substrate to be used as a bioindicator of pollution. This study aimed to determine the Community Structure of Macrozoobenthos and the indicator species of water pollution in the Masang Kecil River around the PT AAI area, West Pasaman Regency. This research was conducted using a purposive sampling method with three sampling points, namely upstream and two downstream points after the Wastewater Treatment Plant (IPAL) and after the confluence of tributaries. Sampling using Surber net size 30 x 30 cm. The results of identifying the types of Bento's organisms as a whole identified as many as 45 species. Most species are found in Class Insecta. Based on the diversity index, the water quality in PT AAI's river basin was classified as unpolluted, and it could be concluded that the benthic condition was in good condition on a scale of 4. In the current study, the Diptera order as an indicator of polluted water was most commonly found among orders from other insect classes, namely, ten species. However, there were nine species from the Order Ephemeroptera as an indicator of clean waters and six species for Trichoptera.

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Environmental DNAVolume 4, Issue 6 p. i-i COVER IMAGEFree Access Cover Image First published: 24 November 2022 https://doi.org/10.1002/edn3.219AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract Photo caption and credit Top Left: A pinto abalone (Haliotis kamtschatkana) sits atop a boulder during an eDNA sampling dive. Credit: Katie Sowul, WDFW Top Middle: A Lesser Sand-Plover (Charadrius mongolus) foraging in a Hong Kong wetland. Photo credit: Hon Shing Fung. Top Right: Ecdyonurus helveticus, Isoperla grammatica and Microterpna sequax (left to right) are three species representing the insect orders Ephemeroptera, Plecoptera and Trichoptera, respectively. The sampling of eDNA allows for a non-invasive monitoring of these important bioindicator taxa. Photo Credit: Florian Altermatt (© Florian Altermatt). Bottom Left: Batagay megaslump in spring 2019, Yakutia, Russian Federation. Photo credit: Alexander Kizyakov Bottom Middle: Part of the experimental setup for studying airborne eDNA: We investigated the diversity and accumulation of airborne eDNA in natural habitats using an experimental setup. Photo by Philip Francis Thomsen Bottom Right: Paint roller used to collect eDNA of targeted organisms (Spotted Lanternfly (Lycorma delicatula)) from trunk and branch surfaces. Credit: Michael Allen Volume4, Issue6November 2022Pages i-i RelatedInformation