The carbon stock stored within the biomass of tree species is vital in the forest ecosystem as it contributes significantly to the carbon balance. In addition, the physicochemical properties of soil play a critical role, in influencing overall ecosystem health. This study has analyzed the carbon stock and influence of seasonal change on soil physicochemical properties along soil depths in the Shivpuri Nagarjun National Park (SNNP), Nepal. The above-ground biomass carbon stock was found to be 227.09 t/ha and below-ground stock was 45.42 t/ha. Tree species Castanopsis tribuloides exhibited the highest values of above and below-ground tree carbon stock. The soil of the study site was sandy loam and slightly acidic. High temperature and moisture in the monsoon season were followed by an increased bulk density during the pre-monsoon with deeper soil layers. The sand, silt, and clay contents did not differ significantly across the seasons and depths. The key soil nutrients, like carbon, total nitrogen, phosphorus, and potassium were high during the monsoon season at the topsoil layer, which gradually declined with increasing depth in all seasons. The study highlights that the total tree carbon stock in the study site is 272.51 t/ha, with significant seasonal and depth-related variations in soil attributes. The monsoon season, characterized by maximum soil moisture and higher concentrations of essential soil components, is crucial in influencing soil physicochemical properties and offers important insights for forest conservation and management. Bangladesh J. Bot. 53(3): 567-576, 2024 (September)