Tunnels with natural ventilation and extraction have become the focus of ventilation research in recent years. It is significant to study the characteristics of smoke in tunnel fires to ensure the safety of people and the tunnel structure. Previous research has mainly focused on natural ventilation in horizontal tunnels, and there are few studies on sloped tunnels. In this paper, we studied the smoke characteristics of natural ventilation extraction in slope tunnel fires both experimentally and theoretically. The small-scale experimental results showed that the position of the fire source, heat release rate (HRR), and the size of the shaft had little effect on the deflection angle of the fire plume. The deflection angle of fire plume was only related to the tunnel slope and increased with the tunnel slope. The slope had no effect on the smoke temperature distribution on the downside of the tunnel, while the smoke temperature on the upside decreased with the increase in the slope. The calculation models of the maximum smoke temperature rise and the smoke temperature distribution were obtained based on the experimental results and theoretical analysis. Compared with the experimental data, the developed semi-empirical models could provide a reliable prediction of smoke temperature.