Maximum Side Research Articles

Insight into Protein Engineering: From In silico Modelling to In vitro Synthesis.

Protein engineering alters the polypeptide chain to obtain a novel protein with improved functional properties. This field constantly evolves with advanced in silico tools and techniques to design novel proteins and peptides. Rational incorporating mutations, unnatural amino acids, and post-translational modifications increases the applications of engineered proteins and peptides. It aids in developing drugs with maximum efficacy and minimum side effects. Currently, the engineering of peptides is gaining attention due to their high stability, binding specificity, less immunogenic, and reduced toxicity properties. Engineered peptides are potent candidates for drug development due to their high specificity and low cost of production compared with other biologics, including proteins and antibodies. Therefore, understanding the current perception of designing and engineering peptides with the help of currently available in silico tools is crucial. This review extensively studies various in silico tools available for protein engineering in the prospect of designing peptides as therapeutics, followed by in vitro aspects. Moreover, a discussion on the chemical synthesis and purification of peptides, a case study, and challenges are also incorporated.

Effect of side ratio on the two-degrees-of-freedom vortex-induced vibrations of the rectangular cross section model

The effect of the mutative side ratio (D/B) on the vortex-induced vibration (VIV) characteristic, aerodynamic characteristics, and the surrounding time-averaged and transient flow field of a rectangular cross section model were simulated numerically. Based on Fluent 19.0 platform, overset grid technology and the fourth-order Runge–Kutta method were used at Re 22 000. First, a rectangular cross section model with D/B = 0.25 was selected, and the simulation method and parameter settings were validated against previous literatures. The subsequent analysis compared and evaluated the effect of side ratio on the VIV response by focusing on statistical values of aerodynamic force coefficients, self-spectra, amplitude ratio, motion trajectory, and phase transition changes for stream-wise and cross-flow directions. Moreover, the study examined the influence of different models at different reduced velocities (Ur) on wake vortex-shedding. The findings suggest that, within a fixed cross-sectional area, a smaller side ratio leads to a weaker VIV characteristic and notably lower aerodynamic performance compared to a larger side ratio. The vortex-shedding mode of the rectangular cross section, particularly with a large side ratio, is less sensitive to changes in Ur compared to the standard square cylinder. An examination of the Reynolds number (Re) effect on the minimum and maximum side ratio models reveals that it has a more pronounced impact on the aerodynamic performance and VIV of the cross-flow when compared to in-line flow. In general, it is noted that larger side ratio model exhibits a stronger sensitivity to the variation of Re.

High power lateral coupled InAs/GaAs quantum dot distributed feedback lasers grown on Si(001) substrates

High-quality single-frequency semiconductor lasers play a key role in silicon optical integrated systems. Combining high density 8-stacked quantum dot (QD) material and low–loss laterally coupled gratings, we here demonstrate a high output power, low noise, and insensitivity to light feedback 1.3 µm InAs/GaAs QD distributed feedback (DFB) laser grown on Si(001) substrates. For a QD DFB laser of a 3 × 1500 µm2 cavity, it exhibits a high single-mode output light power of up to 25 mW at 20 °C and 1.8 mW at 70 °C, respectively and maintains a stable single–mode operation in the entirely measured temperature range with a maximum side mode suppression ratio (SMSR) of 56.5 dB. Furthermore, the laser has an average relative intensity noise value low to –155.9 dB/Hz and a Lorentzian linewidth narrow to 243 kHz. In addition, the laser shows an insensitivity to optical feedback with a feedback level of –24.9 dB. Lastly, a 7-channel QD DFB laser array emitting at wavelengths from 1274.5 nm to 1290.0 nm are also exhibited with all SMSRs of higher than 45 dB. The results achieved here enable a practical single-frequency Si-based light source for the development of high-performance silicon photonic chips.

Semi-decentralized Lyapunov-based formation control of multiple omnidirectional mobile robots

<p>This paper introduces an advanced formation control algorithm based on a Lyapunov approach for coordinating multiple omnidirectional mobile robots in collaborative object transport tasks. The semi-decentralized strategy ensures that the robots maintain a predefined geometric formation, crucial for stability during material transportation, and dynamically adapt to avoid collisions using onboard sensors. Experimental with a physical robot simulator demonstrates successful maintenance of line and triangle formations achieving an average side length maintenance of 1.00 meters with minimal deviation. Quantitative analysis across 30 experimental runs reveals consistent performance, with a maximum side length fluctuation of only 2 centimeters, validating the effectiveness of maintaining formation within a multi-robot system (MRS) framework. The Lyapunov-based approach proves to be an efficient method for cooperative object transport, achieving consistent performance with minimal deviation.</p>

Preparation and Evaluation of Herbal Anti-Acne Face Wash Gel

Aim: To study the preparation of herbal anti-acne face wash gel remedies are more tolerable as well as safer as with side effects than the synthetic ones. We have prepared formulation using herbals. Objectives: Acne is common problem that 85% of the teenagers face today. Inthis study, poly herbal anti-acne face wash gel where prepared using herbal materials. Material and methods: Herbal face wash was prepared from the extract of neem leaves (azadirachta indica), tulsi, lemon juice, aloe Vera, glycerin, carbopol, methyl paraben, trie-thanolamine; sodium lauryl Sulphate is generally used in the preparation of face wash to avoid the maximum side effects

Comparison of various single-disc type furrow openers used in no-till seeders in terms of furrow properties and acting forces

The purpose of this study was to examine the impact of various single-disc type furrow openers used in no-till seeders on furrow aspects and forces acting on the discs at various soil moisture contents, soil compaction levels, and seeding depths in soil bin. Factors included plain, notched, wavy, concave, and fluted types of disc coulters, two soil moisture levels (12-18%), two soil compaction levels (2-3 MPa), and two seeding depths (50-75 mm). According to the results, the plain, notched, and fluted type discs had the lowest vertical, draft, and side force, while the wavy type disc had the maximum vertical force, draft force, and side force and the concave type disc had the highest side force. The vertical, draft, and side force requirements of all furrow openers were found to be lower in loose soils with low moisture content and greater in compacted soils with high moisture content. Fluted, concave, and wavy-type discs produced the best results in terms of the specific vertical, specific draft, and specific side forces, respectively. While the highest furrow cross-sectional area was obtained in the wavy disc, this furrow opener was followed by concave, notched, fluted, and plain-type furrow openers, respectively.

Flexural Strength and Morphological Study of Different Multilayer Zirconia Dental Materials.

Nowadays, yttria (Y3+)-stabilized ZrO2 (Y-TZP) is the most commonly used material in dental prosthetics. Y-TZP dental ceramics are mainly stabilized via the addition of 3 mol% yttrium oxide (Y2O3). These ceramics exhibit excellent mechanical properties, including high flexural strength, fracture toughness, elastic modulus, etc. Some manufacturers have recently introduced a new class of dental materials with multilayer composition with the aim of combining the advantages of adding more or less Y2O3 to the ceramic composition in one Y-TZP material. The flexural strength values of multilayer Y-TZP may vary depending on the dimensions of the specimen, layer distributions, and especially the layer exposed on the maximum tension side, i.e., loading configuration. Although previous studies have examined the flexural strength of separate Y-TZP layers, capturing the flexural strength of multilayer Y-TZP is still challenging. However, one should keep in mind that multilayer flexural strength is important for clinical indications. The objective of this study is to compare the flexural strength of three distinct multilayer translucent Y-TZP materials made up of layers with different Y3+ contents. Rectangular samples (2 mm × 2 mm × 16 mm) were prepared from CAD/CAM discs using the milling machine Programill PM7 (Ivoclar Vivadent AG). Milled bars were tested for flexural strength in a three-point bending test (ISO 6872:2015) using a universal testing machine (Inspekt Duo 5kN; Hegewald & Peschke, Nossen, Germany) at a crosshead speed of 0.5 mm/min. Representative samples of each type of material were selected for quantitative and qualitative analysis of the microstructure. Representative samples of each type of material were selected for structural, mechanical, and microstructural analyses.

Wide-angle non-uniform optical phased array using compact and efficient antenna design

In the need for a more compact and efficient optical phased array with a wide steering beam for LIDAR applications, a wide steering array with high resolution is desirable. However, in the published work, a trade-off is often made for one over another. Apodized grating antennas have shown good efficiency with a compact size and wide beam profile, which improve optical phased array beam steering capability and are also compatible with the CMOS silicon photonics process. A promising studies shows enhancement in steering range with good resolution utilizing a non-uniform optical phased array. In this work, we present two highly efficient optical antennas with 94% and 93.5% upward power at the center frequency for the first and second antenna respectively, exceeding state-of-the-artwork to the best of our knowledge, and wide full-width half maximum of 8.88° x 78.05° and 7.53° x 69.85° in elevation and azimuthal planes, respectively. Both antennas provide a broad bandwidth across the 1400–1700 nm wavelength range with more than 80% efficiency in the S, C, and L bands. To overcome the limited scan ranges and small aperture size, a two-dimensional non-uniform array of 10 × 10 elements is utilized to increase the beam steering capability. A genetic algorithm is used to optimize the position of array elements, resulting in an aliasing-free array with a wide steering range of 160° with beam width 0.5° and consistent −11 dB maximum side lobe level across the steering range.

Development of eco-friendly spectrophotometric methods for analysis of metformin hydrochloride and linagliptin in presence of metformin toxic impurity in their pure and dosage forms: Validation, practicality and greenness studies

Metformin is considered as type 2 diabetes first line treatment according to American Diabetes Association and European Association. But, in some cases, di- or tri - therapy should be prescribed for glycemic management, prevention of the maximum dose side effects and induced effectiveness. Co-administration of Linagliptin with metformin has many benefits on diabetic patients such as decrease the possibility of hypoglycemia. For the first time, novel and reliable techniques were developed and verified for the concurrent quantification of metformin hydrochloride and linagliptin, while accounting for the existence of metformin toxic impurity 1-cyanoguanidine in their pure and dosage forms. Method (A) utilizes the zero-order spectrophotometric approach to quantitatively determine the concentration of linagliptin. The measurements are performed at a wavelength of 295 nm. The double divisor derivative ratio spectrophotometric method is used in Method (B) to measure the amounts of metformin and cyanoguanidine at 252 nm and 219 nm wavelengths, respectively. The spectrophotometric method (C) for determining metformin and cyanoguanidine at 252 nm and 223 nm, respectively, is based on the single divisor derivative ratio-zero crossing technique. The obtained findings were subjected to statistical comparison with the reported method, revealing no statistically significant differences. The Green Analytical Procedure Index (GAPI) and Analytical GREEnness Metric approach (AGREE) determined that these approaches had a high degree of environmental friendliness. Additionally, the proposed strategy was deemed to be practical according to the Blue Applicability Grade Index (BAGI) assessment tool.

An insight into advances and challenges in the development of potential stearoyl Co-A desaturase 1 inhibitors.

Stearoyl-CoA desaturase 1 (SCD1) is one of the key enzymes involved in lipid metabolism, plays a vital role in the synthesis of monounsaturated fatty acids (MUFAs) from saturated fatty acids (SFAs). Due to its promising therapeutic potential in treating metabolic disorders, cancers, and skin diseases there is an increasing interest in the development of novel inhibitors against SCD1. This review comprehensively explores the evolution of potential SCD1 inhibitors, focusing on systemic and liver-targeted inhibitors and discusses their structure-activity relationship (SAR) pattern. Among the various small molecules reported, natural products like sterculic acid have emerged as significant SCD1 inhibitors, highlighting the potential of naturally derived compounds in therapeutic development. This review also addresses the challenges in optimizing pharmacokinetic properties and reducing adverse effects, providing insights into the future directions for the development of potential novel SCD1 inhibitors with maximum therapeutic effect and minimum side effects.

Применение зондирующих ФКМ-сигналов с нулевой зоной автокорреляции для улучшения качества измерений в РСА

For SARs, a polyphase (p-phase, where p is a prime number) probing signal with a zero autocorrelation zone has been synthesized, which is a train of p phase-code shift keyed (PCSK) pulses coded by complementary sequences of a p-ary D-code with additional phase-code shift-keying of the sub-pulses (discretes) of pulses by an M-sequences. To assess the quality of radar images of point targets and extended surfaces, the levels of the maximum side lobe and one-way integrated correlation noise of the autocorrelation function (ACF) are used, respectively. For this purpose, the work carried out a comparative analysis of the total ACFs of the synthesized ensemble of signals with ensembles of orthogonal chirp and PCSK-signals used in adjacent SAR sounding cycles. The work also considers the problem of superimposition of SAR echo signals from different ranges at a relatively high repetition rate of probing pulses. To assess the level of suppression of recurrent interference from point targets and from extended surfaces, the levels of the maximum side lobe and one-sided integral correlation noise of the cross-correlation function (CCF) are used, respectively. For this purpose, the work carried out a comparative analysis of the total CCFs of the synthesized ensemble of signals with ensembles of orthogonal chirp and PCSK- signals used in the SAR aperture synthesis modes. The use of orthogonal chirp and PCSK-signals makes it possible to successfully suppress false signals from bright point targets from adjacent repetition periods. However, the high level of integral correlation noise of the CCF of orthogonal signals distorts radar images of weakly reflecting surfaces located next to bright extended objects. The indicators of the total correlation characteristics of the synthesized ensemble of signals exceed the corresponding indicators of the total correlation characteristics of the ensemble of orthogonal PCSK-and chirp signals, both without mismatch and with Doppler frequency mismatch. This makes it possible to successfully use an PCSK-signal with a zero autocorrelation zone to improve the quality of radar images and suppress recurrent range interference in SAR.

High single-mode selectivity V-cavity tunable semiconductor laser based on GaAs

With the progress of integrated optoelectronic devices over recent decades, the semiconductor research has increasingly focused on tunable semiconductor lasers featuring a compact structure and simplified fabrication. In this manuscript, we present a proposal for a V-Cavity tunable semiconductor Laser (VCL) at a center wavelength of 940 nm. The theoretical analysis encompasses the threshold conditions of the laser, considering various cavity length differences within the waveguide layer structure. Moreover, we showcase a VCL with a waveguide width of 3 μm and a channel interval of 0.6 nm. Through the optimization of polynomial parameters associated with the curved waveguide, the VCL attains a transmittance exceeding 99.4 %. Additionally, we address the determination of optimal parameter ranges and solve the optical field distribution within the half-wave coupler. The simulation results exhibit that the half-wave coupler, employing optimized parameters, achieves remarkable single-mode selectivity when operating at a central wavelength of 940 nm. Notably, a maximum side mode suppression ratio (SMSR) of 47.9 dB is attainable with a half-wave coupler length of 45 μm and a waveguide gap of 1.7 μm.

Multi-wavelength sub-100 Hz linewidth Brillouin fiber laser with cascaded SBS polarization parity-time symmetry

A multi-wavelength sub-100 Hz linewidth Brillouin fiber laser (BFL) with cascaded stimulated Brillouin scattering (SBS) polarization parity-time (PT) symmetry is proposed and experimentally investigated. Multi-order Stokes wave generation using a structure consisting of a main fiber ring (MFR) and a sub fiber loop (SFL). The MFR is used to excite the Stokes laser and to guarantee single longitudinal mode (SLM) operation of the generated stokes laser of each order and the SFL cascades each Stokes lasing emission to act as a Brillouin pump for the next order of Stokes generation. A Sagnac loop in the MFR consisting of optical coupler (OC), polarization beam splitter (PBS) and two polarization controllers (PCs), is used to achieve cascaded SBS polarization PT symmetry. By tuning the PC to control the polarization state of each order linearly polarized light injected into PBS at each order, thus controlling the gain and loss of the loop corresponding to the two backpropagating light waves. When the gain and loss of the coupling loops are the same in magnitude and greater than the coupling coefficient, the cascaded SBS polarization PT symmetry is broken, resulting in SLM operation for each order of Stokes waves. Experimental results reveal that thirteen Stokes waves were obtained from two different output ports at an erbium-doped fiber amplifier (EDFA) output power of 28 dBm. The measured linewidths at — 20 dB power were 0.34, 0.39, 0.33, 0.4, 0.39 and 0.43 kHz for the 1st-6th order Stokes emission, corresponding to linewidths of 17.1, 19.6, 16.6, 20.1 19.6 and 21.6 Hz, respectively, while the maximum side mode suppression ratios (SMSRs) were measured to be 47, 45, 49, 44, 41 and 41 dB for the 1st-6th order Stokes emission. Within 60 min of the stability experiment, the wavelength and power stability were ±2 pm and ±0.6 dB, respectively. Compared with previous studies, the present design guarantees the operation of SLM for each order of Stokes waves with sub-100 Hz laser linewidth.

Study on thermo-mechanical properties of energy piles based on the improved exponential model

To further investigate the effects of temperature on the bearing capacity of the energy pile, the influence of soil temperature was considered and the pile top displacement corresponding to the ultimate side friction resistance of the energy pile was obtained. An improved exponential model that accounts for the influence of pile-soil temperature was developed. The feasibility of the model was verified through an indoor model test and numerical simulation. The results indicate that the error between the improved exponential model and the indoor model test was smaller compared with the numerical simulation. The maximum side friction error between the improved exponential model and the model test reached 0.8 % and 4.6 % at the end of the heating and cooling stages, respectively. The maximum additional thermal stress error between the improved model and the model test was 1.3% and 3.9% at the end of the heating and cooling stages, respectively. The error of the traditional and improved exponential model of additional thermal stress was 16 %. It is established that the improved exponential model was proved suitable for thermo-mechanical coupling analysis of energy piles. The temperature effect of the soil should be considered in the investigation of the energy pile.

PEDODONTİ KLİNİĞİNE AĞRI ŞİKAYETİYLE BAŞVURAN HASTALARDA ANALJEZİK KULLANIMININ DEĞERLENDİRİLMESİ

AIM: The aim of this study was to evaluate the use of analgesics, the doses used and the level of knowledge of patients about analgesics in patients presenting with pain to the pediatric dentistry clinic.
 MATERIALS AND METHODS: A questionnaire was applied to the parents of 120 children aged 3-15 years who referred to ……. University Faculty of Dentistry, Department of Pediatric Dentistry, with pain. The questionnaire included following data; the duration of the pain, the type and dose of the analgesic used, the intensity of the pain before and following analgesic use based on the VAS scale, knowledge about the maximum daily dose of the analgesic used. The data were analyzed statistically with Chi-square and Mann-Whitney U test.
 RESULTS: One hundred and twenty patients were included in the study. The mean age of children using analgesics was 8.2±2.6 years. Paracetamol was the most frequently used analgesic in this population (64,2%) followed by ibuprofen (45%). 18,3% of children were taking more than one analgesic. Although 66,6% of the parents were unaware of the maximum daily dose, only three of the children exceeded this limit (using paracetamol). Also 65% of the parents were unaware of side effects of the analgesics. Parents taking analgesics on medical advice were not more aware of the maximum daily dose. 
 CONCLUSIONS:
 A large portion of the parents were unaware of the maximum daily dose and side effects of analgesics used in children. Dentists treating cases with pain should inform patients about the use of analgesics, side effects and maximum doses.

Side effects and short effects of using three common mouthwashes on oral health and quality of life: A quasi-experimental study.

Antiseptic mouthwashes are useful adjuncts to daily brushing and flossing for the control of dental plaque and gingivitis. The objective of the present study was to compare the effect of three mouthwashes: chlorhexidine, essential oils and cetylpyridinium chloride on oral health-related quality of life, gingival health, tongue coating and also to compare their potential side effects after 2-week consumption. Sixty participants were enrolled in this study and were divided into four groups (three mouthwash groups plus no mouthwash group). At the beginning, scaling and cleaning were performed and the following parameters were recorded: oral health impact profile (OHIP-5) questionnaire, tongue coating index, modified gingival index, calculus surface index, modified Lobene stain index and a questionnaire for side effects. Each group has followed up in 2 weeks. Data were analysed using paired t-test, ANOVA, Fisher's exact test and Pearson chi-square. At the end of second week, all three mouthwashes significantly improved OHIP-5 score and reduced modified gingival index whereas essential oils and cetylpyridinium chloride significantly reduced tongue coating index when compared to control group (p < 0.05). The differences between four groups were significant for calculus formation, dental staining and reported burning sensation and changes of taste sensation and perception (p < 0.05). The use of all three mouthwashes has been effective in controlling and reducing gingivitis and tongue coating; however, it appeared that essential oils has the minimum and Chlorhexidine has the maximum side effects. Moreover, the use of all three mouthwashes has been examined to improve the quality of life. Overall, essential oils mouthwash has the best performance among these three mouthwashes.

Akış Parametrelerinin Termoelektrik Modüldeki Isı Transferi ve Sıcaklık Dağılımı Üzerine Etkileri

The effects of the hot air inlet velocity and hot air inlet temperature on the hot side temperature, the temperature difference between the hot and cold sides, and the heat transfer rate on the hot side of a thermoelectric module (TEM) were investigated by computational fluid dynamics (CFD) analyzes. As a result of CFD analysis, the maximum hot side temperature, the temperature difference between the hot and cold sides, and the average heat transfer rate on the hot side of the TEM are found to be 274.9 °C, 70.4 °C, and 33.8 W, respectively, at 15 m/s of hot air inlet velocity. Besides, the maximum hot side temperature, the temperature difference between the hot and cold sides, and the average heat transfer rate on the hot side of the TEM are determined to be 432.8 °C, 114.9 °C, and 55.1 W, respectively, for 800 °C of hot air inlet temperature at 15 m/s of hot air inlet velocity. As a result, increasing the hot air inlet velocity and inlet temperature increases the hot side temperature, the temperature difference between the hot and cold sides, and the heat transfer rate on the hot side of the TEM.

The influence of vehicle body roll motion on aerodynamic characteristics under crosswind condition

PurposeThe purpose of this study is investigate the transient aerodynamic characteristics of high-speed vehicle with body roll motion under crosswind condition to improve aerodynamic stability.Design/methodology/approachAn overset mesh was used to simulate the rolling motion of the vehicle body. A wind tunnel experiment was conducted to validate the numerical method.FindingsThe results revealed that the vehicle’s aerodynamic characteristics changed periodically with the body’s periodic motion. In the absence of crosswind, the pressure distribution on the left and right sides of the vehicle body was symmetrical, and the speed streamline flowed to the rear of the vehicle in an orderly manner. The maximum aerodynamic lift observed in the transient simulation was −0.089, which is approximately 0.70 times that of the quasi-static simulation experiment. In addition, the maximum aerodynamic side force observed in the transient simulation was 0.654, which is approximately 1.25 times that of the quasi-static simulation experiment.Originality/valueThe aerodynamic load varies periodically with the vehicle body’s cyclic motion. However, the extreme values of the aerodynamic load do not occur when the vehicle body is at its highest or lowest position. This phenomenon is primarily attributed to the mutual interference of airflow viscosity and the hysteresis effect in the flow field, leading to the formation of a substantial vortex near the wheel. Consequently, the aerodynamic coefficient at each horizontal position becomes inconsistent during the periodic rolling of the vehicle body.

Analysis of the force of new energy vehicle drive bridge

In recent years, the new energy vehicle industry has developed rapidly. As one of the important systems of cars, the research on the driving system of new energy vehicles is also of great significance. This article is mainly for the four typical work conditions of the car-driven axle case: the active conditions, the maximum traction conditions, the operating conditions and the maximum side force of the power of the force of the power of the car. According to the analysis conducted, the evaluation of a car's performance varies depending on its load conditions. When the car carries a full load of static weight, the primary focus is assessing the driving force generated by the axle case. Conversely, when the car operates at its maximum traction capacity, the key aspect under scrutiny is the vertical force exerted by the ground and the tires on the vehicle's exterior torque. In the case of a fully-loaded car, the assessment of lateral sliding primarily involves examining the side force produced by the axle case. Additionally, this article utilizes a concise driver system diagram to analyze the advantageous layout of the drive system in new energy vehicles. The obtained result has important guiding significance for the optimized design of the new energy vehicle drive bridge.

Design of Fourier ptychographic illuminator for single full-FOV reconstruction.

Fourier ptychographic microscopy (FPM) is a spatial-temporal-modulation high-throughput imaging technique via a sequential angle-varied LED illumination. Therefore, the illuminator is one of the key components and the design of this illuminator is significant. However, because of the property of spherical wave, partial coherence, and aperture-induced vignetting, the acquired images must be processed in blocks first, and rely on parallel reconstruction via a graphics processing unit (GPU). The high cost makes it unappealing compared with commercial whole slide imaging system via a low-cost central processing unit (CPU). Especially, the vignetting severely destroys the space-invariant model and induces obvious artifacts in FPM, which is the most difficult problem. The conventional method is to divide the field of view (FOV) into many tiles and omit those imperfect images, which is crude and may discards low frequency information. In this paper, we reevaluated the conditions of vignetting in FPM. Through our analysis, the maximum side length of FOV is 0.759 mm for a single full-FOV reconstruction via a 4×/0.1 NA objective and a 4 mm spacing LED array in theory, while almost 1.0 mm can be achieved in practice due to the tolerance of algorithm. We found that FPM system can treat the vignetting coefficient Vf below 0.1 as brightfield images and Vf lager than 0.9 as darkfield images, respectively. We reported an optimized distribution for designing an illuminator without vignetting effect according to the off-the-shelf commercial products, which can reconstruct full FOV in one time via a CPU. By adjusting the distribution of LED units, the system could retrieve the object with the side length of FOV up to 3.8 mm for a single full-FOV reconstruction, which achieves the largest FOV that a typical 4×/0.1 NA objective with the field number of 22 mm can afford.