In recent years, research on interfacial photothermal water evaporation has been thriving. Due to its inherent porosity, exceptional hydrophilicity, and renewable characteristics, wood has garnered significant attention as a material for interfacial photothermal evaporation absorbers. In order to enhance the cellular channels of poplar and improve its water migration capacity, Trichoderma spiralis was selected to inoculate and culture poplar specimens from different sections for 3, 5, and 7 weeks. Simultaneously, a solar radiation intensity of 1 kW·m⁻2 was simulated to perform photothermal evaporation tests on the specimens. This validated the water migration capabilities of different sections of poplar treated with Trichoderma spiralis under light and heat exposure. The characteristic changes were analyzed using electron microscope scanning, infrared spectrum analysis, X-ray photoelectron spectroscopy analysis, surface infiltration performance, and automatic specific surface porosity. The results suggested that the moderate degradation of cellulose and hemicellulose in poplar by Trichoderma spiralis could dredge the cell channels and improve the permeability of poplar, particularly with regard to lateral permeability. The maximum photothermal evaporation rate of the poplar specimen reached 1.18 kg m⁻2 h⁻1, while the evaporation efficiency increased to 72.2%.