Inhibition Of Maximum Germination Research Articles

Mycoflora impact on germination and vigour of bottle gourd (Lagenaria siceraria) seeds and its management through different plant protectants

Bottle gourd is an important cucurbitaceous crop. The impact of 14 species from eight fungal genera on bottle gourd seeds was studied through in vitro tests. Bottle gourd seeds were inoculated with each isolate separately and subjected to various germination tests. Maximum germination inhibition was upto 69.3% due to Fusarium proliferatum. Inoculation of seeds with F. proliferatum significantly reduced the germination speed index to 2.69. The seedling vigour index I for seeds inoculated with F. proliferatum and F. incarnation-equiseti (species complex) was 860 and 861, respectively, significantly different from that of non-inoculated seeds having a vigour index of 1791. The seedling vigour index II of seeds inoculated with F. proliferatum was 1818, significantly lower than the control (4631), indicating that the F. proliferatum isolate was highly virulent among all the isolates. Amongst seven plant protectants evaluated for their efficacy as seed dressers against F. proliferatum, propioconazole (25% EC), copper oxychloride (50% WP) and Mancozeb (75% WP) were effective in reducing infection. Germination percentage was highest for seeds treated with the bioagent Trichoderma harzianum 1% WP.

Formulation, Characterization and Evaluation of Encapsulated Bioherbicide on Echinochloa cruss galli and Phalaris minor

Weeds management under organic agriculture demands organic herbicides / bioherbicides.to address this issue an experiment was conducted in Centre of Environment Sciences and Technology, Central University of Punjab, Bathinda to study the effects of different concentration and bioassay of encapsulated essential oil extracted (EOs) from Callistemon viminalis on Echinochloa cruss galli and Phalaris minor under lab conditions. Encapsulation efficiency of gum arabic and maltodextrin (GAMD) EOs increased from 26 to 31% for 4 to 8% of EOs concentration. The sizes of all the particles were found in the range of 1-10 μm. The reduced size in case of EOs loaded GAMD-EOs encapsulates may be due to the application of spray drying method used during the preparation. Maximum germination inhibition was observed with P. minor as compared to the E. crus-galli L. The probable reason behind this may be the relatively smooth seed coat, smaller weight to volume ratio of P. minor as compared to the E. crus-galli L. Among all the treatments basal application of encapsulates with 8 % essential oil was found more lethal and result in maximum phyto-toxicity by registering less shoot length and root length and fresh biomass weight. Also, the individual constituents of the EOs can be explored for their use as herbicides and then their encapsulated formulations can be used for scale up in the field conditions.

Differential biochemical response in resistant and susceptible barley (Hordeum vulgare) cultivars upon covered smut (Ustilago hordei) infection

Covered smut of barley is an important seed borne disease caused by Ustilago hordei. This disease has been a serious threat and consequently necessitated understanding of barley-smut resistance mechanism. The present study focussed on the biochemical changes in three barley cultivars viz., PL426 (resistant), PL891 (moderately susceptible) and VJM201 (susceptible), during the infection process of covered smut. Dehulled seeds of each of these varieties were artificially inoculated with U. hordei (108 teliospore suspension) prior to germination and the seedling samples were collected at a day interval till 8 days after germination (DAG) for the study. The results revealed a significant increase in antioxidative activities of defense enzymes [peroxidase (POD), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO)] and total phenolic content in inoculated samples than the uninoculated ones and the increase was more pronounced in resistant line (PL426) than susceptible (VJM201) and moderately susceptible (PL891) ones. PL426 recorded maximum activity of POD (86.70 units min−1 g−1 fw) and PPO (89.30 units min−1 g−1 fw) which leads to the accumulation of more phenols (0.64 mg/g). The biochemical results were further confirmed by observing the effect of aqueous extract of resistant and susceptible varieties on germination of teliospores of U. hordei. Maximum germination inhibition (98%) of teliospores was exhibited by PL426 when 100% concentration was used whereas the inhibition in case of PL891 and VJM201 was only 18% and 2%, respectively at the same concentration. The study has highlighted the changes in the most efficient antioxidative system that could limit the cellular damage caused by U. hordei.

Seed germination ecology of Bidens pilosa and its implications for weed management

It is now widely recognized that Bidens pilosa has become a problematic broadleaf weed in many ecosystems across the world and, particularly in the light of recent climate change conditions, closer management strategies are required to curtail its impact on agricultural cropping. In this investigation, experiments were conducted to evaluate the effect of environmental factors on the germination and emergence of B. pilosa, and also on the response of this weed to commonly available post-emergence herbicides in Australia. The environmental factors of particular interest to this current work were the effect of light and temperature, salinity, burial depth and moisture on B. pilosa since these are key management issues in Australian agriculture. In addition, the effects of a number of commonly used herbicides were examined, because of concerns regarding emerging herbicide resistance. In the tested light/dark regimes, germination was found to be higher at fluctuating day/night temperatures of 25/15 °C and 30/20 °C (92–93%) than at 35/25 °C (79%), whilst across the different temperature ranges, germination was higher in the light/dark regime (79–93%) than in complete darkness (22–38%). The standard five-minute temperature pretreatment required for 50% inhibition of maximum germination was found to be 160 °C, and it was further shown that no seeds germinated at temperatures higher than 240 °C. With regard to salinity, some B. pilosa seeds germinated (3%) in 200 mM sodium chloride (NaCl) but all failed to germinate at 250 mM NaCl. Germination declined from 89% to 2% as the external osmotic potential decreased from 0 to −0.6 MPa, and germination ceased at −0.8 MPa. Seeding emergence of B. pilosa was maximum (71%) for seeds placed on the soil surface and it was found that no seedlings emerged from a depth of 8 cm or greater. A depth of 3.75 cm was required to inhibit the seeds to 50% of the maximum emergence. In this study, application of glufosinate, glyphosate and paraquat provided commercially acceptable control levels (generally accepted as >90%) when applied at the four-leaf stage of B. pilosa. However, none of the herbicide treatments involved in this study provided this level of control when applied at the six-leaf stage. In summary, B. pilosa germination has been clearly shown to be stimulated by light and thus its emergence was greatest from the soil surface. This suggests that infestation from this weed will remain as a problem in no-till conservation agriculture systems, the use of which is increasing now throughout the world. It is intended that information generated from this study be used to develop more effective integrated management programs for B. pilosa and similar weeds in commercial agricultural environments which are tending toward conservation approaches.

Seed Germination, Seedling Emergence, and Response to Herbicides of Triquetrous Murdannia (Murdannia triquetra) in Rice

Triquetrous murdannia is an annual weed commonly found in rice fields in China. Laboratory and screenhouse experiments were carried out to determine the effect of light, temperature, osmotic and salt stress, seed burial depth, amount of rice residue, and depth of flooding on seed germination and seedling emergence of triquetrous murdannia and to evaluate the response of this weed to commonly available POST herbicides in China. Germination was greater than 93% under a wide day/night temperature range of 20/10 to 30/20 C in the light/dark regime. The time to onset of germination decreased as temperature increased. Germination was slightly stimulated when seeds were placed in light/dark conditions compared with seeds placed in the dark. The osmotic potential and NaCl concentration required for 50% inhibition of maximum germination were −0.5 MPa and 122 mM, respectively. The highest germination (68%) was observed from seeds sown on the soil surface, but decreased with increasing burial depth. Only 7% of seedlings emerged from a depth of 4 cm, and no seedlings emerged from seeds buried deeper than 6 cm. Seedling emergence decreased from 93 to 35% with increasing quantity of rice residue (1 to 6 103kg ha−1) applied on the soil surface. Seedling emergence was reduced by 40, 48, 64, and 70% at flooding depths of 1, 2, 4, and 6 cm, respectively, for the seeds sown on the soil surface. Fluroxypyr and MCPA herbicides provided 100% control of triquetrous murdannia at the 2- to 6-leaf stages; however, to achieve 100% control with bispyribac-sodium, MCPA+bentazone or MCPA+fluroxypyr, herbicides had to be applied by the 4-leaf stage. The results of this study could help in developing more sustainable and effective integrated weed management strategies for the control of triquetrous murdannia in rice fields in China.

Seed Germination, Seedling Emergence, and Response to Herbicides of Wild Bushbean (Macroptilium lathyroides)

Experiments were conducted in laboratory and screenhouse to determine the effects of light, temperature, salt and water stress, seed burial depth, and amount of rice residue on seed germination and seedling emergence of wild bushbean and to evaluate the response of that weed to commonly available POST herbicides in the Philippines. Germination (81 to 85%) was not influenced by light; however, it was higher at 30/20 C (88%) than at 25/15 C (82%) and 35/25 C (80%) day/night temperatures. The sodium chloride and osmotic potential required for 50% inhibition of maximum germination were 149 mM and −0.28 MPa, respectively. Seedling emergence (81%) was similar for seeds placed on the soil surface and for seeds buried up to 4 cm deep. No seedlings emerged from a burial depth of 8 cm. Rice residue applied on the soil surface up to 8 t ha−1did not affect seedling emergence (72 to 80%) of wild bushbean. The herbicide bentazon provided 100% control of wild bushbean when applied at the three- to five-leaf stages. However, to achieve 100% control by 2,4-D and metsulfuron-methyl + chlorimuron-ethyl, herbicides had to be applied at the three-leaf stage. The information gained from this study can help in developing components of integrated weed management programs for wild bushbean. A deep-tillage operation to bury weed seeds below their maximum depth of emergence and early application of an effective POST herbicide can help in managing this weed in rice fields.

English

Laboratory studies on the seed powder and water extract of seeds of Cassia tora and Cassia uniflora on the germination of Parthenium seeds was studied. Seed powders of both weeds were much inhibitory on seed germination of Parthenium than the water extract. Seed powder of Cassia tora showed maximum germination inhibition of 98% and water extract of 67%, whereas seed powder of C. uniflora showed germination inhibition of 95% and water extract of 61%. The results showed that the C. tora seed powder and water extract of its seeds showed much inhibitory effect than C. uniflora on Parthenium. Key words: Cassia tora, Cassia uniflora, seed extract; Parthenium hysterophorus.

Factors affecting Cyperus difformis seed germination and seedling emergence

Specific knowledge about the dormancy, germination, and emergence patterns of weed species aids the development of integrated management strategies. Laboratory studies were conducted to determine the effect of several environmental factors on seed germination and seedling emergence of Cyperus difformis. Germination of freshly harvested seeds was inhibited by darkness; however, when seeds were subsequently transferred to complete light they germinated readily. Our results showed that 2 wk of cold stratification overcome the light requirement for germination. Seeds of C. difformis were able to germinate over a broad range of temperatures (25/15, 30/20, 35/25, and 40/30 ºC day/night). The response of germination rate to temperature was described as a non-linear function. Based on model outputs, the base, the optimum and the ceiling temperatures were estimated as 14.81, 37.72 and 45 ºC, respectively. A temperature of 120 ºC for a 5 min was required to inhibit 50% of maximum germination. The osmotic potential and salinity required for 50% inhibition of maximum germination were -0.47 MPa and 135.57 mM, respectively. High percentage of seed germination (89%) was observed at pH=6 and decreased to 12% at alkaline medium (pH 9) pH. Seeds sown on the soil surface gave the greatest percentage of seedling emergence, and no seedlings emerged from seeds buried in soil at depths of 1 cm.

Seed Germination Ecology of Feather Lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes

Feather lovegrass [Eragrostis tenella (L.) Beauv. Ex Roemer & J.A. Schultes] is a C4 grass weed that has the ability to grow in both lowland and upland conditions. Experiments were conducted in the laboratory and screenhouse to evaluate the effect of environmental factors on germination, emergence, and growth of this weed species. Germination in the light/dark regime was higher at alternating day/night temperatures of 30/20 °C (98%) than at 35/25 °C (83%) or 25/15 °C (62%). Germination was completely inhibited by darkness. The osmotic potential and sodium chloride concentrations required for 50% inhibition of maximum germination were -0.7 MPa and 76 mM, respectively. The highest seedling emergence (69%) was observed from the seeds sown on the soil surface and no seedlings emerged from seeds buried at depths of 0.5 cm or more. The use of residue as mulches significantly reduced the emergence and biomass of feather lovegrass seedlings. A residue amount of 0.5 t ha-1 was needed to suppress 50% of the maximum seedlings. Because germination was strongly stimulated by light and seedling emergence was the highest for the seeds sown on the soil surface, feather lovegrass is likely to become a problematic weed in zero-till systems. The knowledge gained from this study could help in developing effective and sustainable weed management strategies.

Seed germination and seedling emergence of velvetleaf (Abutilon theophrasti) and Barnyardgrass (Echinochloa crus-galli)

Abutilon theophrasti and Barnyardgrass (Echinochloa crus-galli) are major weeds that affect cropping systems worldwide. Laboratory and greenhouse studies were conducted to determine the effects of temperature, pH, water and salinity stress, and planting depth on seed germination and seedling emergence of Velvetleaf and Barnyardgrass. For Velvetleaf, the base, optimum and ceiling germination temperatures were estimated as 5, 35 and 48 ºC, respectively. Seed germination was sensitive to drought stress and completely inhibited by a potential of -0.6 MPa, but it was tolerant to salinity. Salinity stress up to 45 mM had no effect on the germination of Velvetleaf, but germination decreased with increasing salt concentration. Drought and salinity levels for 50% inhibition of maximum germination were -0.3 MPa and 110 mM, respectively. Seed germination of Velvetleaf was tolerant to a wide range of pH levels. For Barnyardgrass, the base, optimum and ceiling germination temperatures were estimated as 5, 38 and 45 ºC, respectively. Seed germination was tolerant to drought stress and completely inhibited by a potential of -1.0 MPa. Salinity stress up to 250 mM had no effect on seed germination. Drought and salinity levels for 50% inhibition of maximum germination were -0.5 MPa and 307 mM, respectively. A high percentage of seed germination was observed at pH=5 and decreased to 61.5% at acidic medium (pH 4) and to 11% at alkaline medium (pH 9). Maximum seedling emergence of Velvetleaf and Barnyardgrass occurred when the seeds were placed on the surface of the soil or at a depth of 1 cm.

Isabgol (Plantago ovata Forsk) seed germination and emergence as affected by environmental factors and planting depth

Isabgol (Plantago ovata Forsk) seed germination and emergence in response to drought (Polyethylene glycol 8000) and salinity stress (NaCl), temperature, pH and planting depth were studied in laboratory and greenhouse experiments. Base, optimum and ceiling germination temperature were estimated as 3.35, 21.24 and 35.04 o C, respectively. Isabgol seed is rather tolerant to low water potential and high salt stress. Salinity stress up to 200 mM had no effect on Isabgol seed germination, but the germination decreased by increasing the salt concentration. The drought and salinity required for 50% inhibition of maximum germination were 328 mM and - 1.24 MPa, respectively. High percentage of seed germination (>93%) was observed at pH=4-6 and declined to 52-58% at alkaline (pH 7-9) pH. Maximum seedling emergence occurred when the seeds were planted on the soil surface and decreased with increasing the depth of planting; no seed emerged from depth of 3 cm.

Threelobe Morningglory (Ipomoea triloba) Germination and Response to Herbicides

Experiments were conducted in the laboratory and screenhouse to determine the effects of scarification; alternating day/night temperatures; light, salt, and water stress; seed burial depth; and rice residue on seed germination and seedling emergence of threelobe morningglory, and to evaluate the response of this weed to commonly available POST herbicides in the Philippines. Germination was stimulated by seed scarification, suggesting that inhibition of germination in this species is mainly due to the hard seed coat. Germination of the scarified seeds was not influenced by the tested temperatures (alternating day/night temperatures of 25/15, 30/20, and 35/25 C) and light. The concentrations of sodium chloride, ranging from 0 to 250 mM, did not influence germination of the scarified seeds of threelobe morningglory. The osmotic potential required for 50% inhibition of maximum germination was −0.35 MPa, although some seeds germinated at −0.6 MPa. Seedling emergence was greatest for the seeds placed on the soil surface (96%), and emergence declined with increased burial depth in soil. The burial depth required for 50% inhibition of maximum emergence was 2.8 cm. No seedlings emerged from a burial depth of 6 cm or greater. Residues of up to 6 Mg ha−1on the soil surface did not influence seedling emergence of threelobe morningglory. The herbicide 2,4-D at 400 g ai ha−1provided excellent control of threelobe morningglory when applied at the four-leaf (100%) and six-leaf (97%) stages. However, at the eight-leaf stage, percent control was reduced to 67% and herbicide rate had to be increased twofold to achieve 95% control. The information gained from this study could contribute to developing components of integrated weed management strategies for threelobe morningglory. Soil inversion by tillage to bury weed seeds below their maximum depth of emergence and early application of an effective POST herbicide could serve as important tools for managing threelobe morningglory.

Crowfootgrass (Dactyloctenium aegyptium) Germination and Response to Herbicides in the Philippines

Crowfootgrass, a C4species, is one of the principal weeds of dry-seeded rice in Asia. Weed management decisions for this species can be derived from knowledge of its seed germination biology. Experiments were conducted in the laboratory and screenhouse to determine the effects of light, alternating day/night temperatures, water stress, seed burial depth, and rice residue on seed germination and seedling emergence of crowfootgrass and to evaluate the response of this weed to commonly available selective POST herbicides in the Philippines. Light stimulated seed germination, but it was not an absolute requirement for germination. Germination in the light/dark regime was greater at alternating day/night temperatures of 25/15 C (92%) than at 30/20 (70%) or 35/25 C (44%). The osmotic potential required for 50% inhibition of maximum germination was −0.23 MPa, although some seeds germinated at −0.6 MPa. Seedling emergence was greatest for the seeds placed on the soil surface (64%), and emergence declined with increased burial depth in soil. No seedlings emerged from a burial depth of 6 cm or greater. Seedling emergence of crowfootgrass was reduced by the addition of rice residue to the soil surface at rates equivalent to 4 to 6 Mg ha−1. Fenoxaprop-p-ethyl + ethoxysulfuron at 45 g ai ha−1provided excellent control of crowfootgrass when applied at the four- (99%) and six-leaf (86%) stage. The information gained from this study could contribute to developing components of integrated weed management strategies for crowfootgrass. Soil inversion by tillage to bury weed seeds below their maximum depth of emergence, use of crop residue as mulch, and early application of an effective POST herbicide could serve as important tools for managing crowfootgrass.

Seed Germination Ecology of Itchgrass (Rottboellia cochinchinensis)

Itchgrass is a weed of many crops throughout the tropics and one of the most important grass weeds of rainfed rice. Experiments were conducted in the laboratory and screenhouse to determine the effects of light, alternating day/night temperatures, high temperature pretreatment, water stress, seed burial depth, and rice residue on seed germination and seedling emergence of itchgrass in the Philippines. Two populations were evaluated and the results were consistent for both populations. Germination in the light/dark regime was greater at alternating day/night temperatures of 25/15 C than at 35/25, 30/20, or 20/10 C. Light was not a requirement for germination, but a light/dark regime increased germination by 96%, across temperature and population. A 5-min high temperature pretreatment for 50% inhibition of maximum itchgrass germination ranged from 145 to 151 C with no germination when seeds were exposed to ≥ 180 C. The osmotic potential required for 50% inhibition of maximum germination was −0.6 MPa for itchgrass, although some seeds germinated at −0.8 MPa. Seedling emergence was greatest for seeds placed on the soil surface, and emergence declined with increasing soil burial depth; no seedlings emerged from seeds buried at 10 cm. The addition of rice residue to soil surface in pots at rates equivalent to 4 to 6 Mg ha−1reduced itchgrass seedling emergence. Since seedling emergence was greatest at shallow depths and germination was stimulated by light, itchgrass may become a problem in systems where soil is cultivated at shallow depths. Knowledge gained in this study could contribute to developing components of integrated weed management strategies for itchgrass.

Influence of environmental factors on seed germination and seedling emergence of yellow sweet clover (Melilotus officinalis)

Laboratory and greenhouse experiments were conducted to determine the effects of drought and salinity stress, temperature, pH and planting depth on yellow sweet clover (Melilotus officinalis) germination and emergence. Base, optimum and ceiling germination temperatures were estimated as 0, 18.47 and 34.60 ºC, respectively. Seed germination was sensitive to drought stress and completely inhibited at a potential of -1 MPa, but it was tolerant to salinity. Salinity stress up to 90 mM had no effect over the M. officinalis seed germination, but the germination decreased by increasing the salt concentration. The drought and salinity required for 50% inhibition of maximum germination were 207 mM and -0.49 MPa, respectively. High percentage of seed germination (>92%) was observed at pH = 5-6 and decreased to 80% at acidic medium (pH 4) and to 42% at alkaline medium (pH 9) pH. Maximum seedling emergence occurred when the seeds were placed at 2 cm depth and decreased when increasing the depth of planting; no seed emerged from depths of 10 cm.

Phytotoxicity of lemon-scented eucalypt oil and its potential use as a bioherbicide

The effect of volatile oil from leaves of Eucalyptus citriodora against some plant species viz. Triticum aestivum, Zea mays, Raphanus sativus, Cassia occidentalis, Amaranthus viridis and Echinochloa crus-galli was investigated. In a laboratory bioassay seed germination of test plants was significantly reduced in response to the different concentrations of the eucalypt oil. Maximum germination inhibition was observed with A. viridis, whereas least effect was seen on R. sativus. Based on the germination response, dose-response curve was generated and LC 50 values were calculated. It was maximum for R. sativus whereas minimum for A. viridis. Further, seedling growth of the test plants and the chlorophyll content in the treated seedlings was significantly reduced at concentrations 0.12 and 0.3 mg/l. Not only the initial growth, but also the spray treatment on the 4-week-old mature plants of two weedy species viz. C. occidentalis and E. crus-galli adversely affected the chlorophyll content and cellular respiration, thereby indicating the adverse effect of eucalypt oil on the photosynthetic machinery and the energy metabolism of the target plants. Based on the study, it is concluded that volatile oil from E. citriodora is phytotoxic and could be utilized as bioherbicide for future weed management programmes.

Effect of Age of Cocomposted MSW And Biosolids on Weed Seed Germination

The influence of municipal solid waste (MSW) and biosolids compost maturity on germination of several weed species seeds was evaluated. Ivyleaf morning glory (Ipomoea hederacea L.), barnyard grass (Echinochloa crus-galli L.), common purslane (Portulaca oleracea L.), and corn (Zea mays L.) were selected as plant indicators to determine the compost maturity stage with maximum germination inhibition. Extracts were prepared from immature (three day-old, four week-old, eight week-old), and mature (one year-old) composts. Extract from eight week-old compost decreased percentage germination, root growth, and germination index (a combination of germination percentage and root growth), and increased mean days to germination of each indicator specie. Extract from eight week-old compost was evaluated for effect on germination percentage of 14 economically important weed species. Extract from eight week-old compost inhibited germination of most weed species, except yellow nutsedge (Cyperus esculentus L.) for which tubers were used as propagules. Extracts from immature (three day, four week, and eight week-old) compost with acetic acid concentrations of 2474, 1790, and 1776 mg·kg−1, respectively, delayed and reduced germination percentage of important economic weed species. These results suggest that immature MSW-biosolids compost might be utilized as an alternative method of weed control in the alley-ways of row crops.

Municipal Solid Waste (MSW) Compost Maturity Influence on Weed Seed Germination

The influence of compost (derived from MSW and biosolids) maturity on seed germination of several weed species was evaluated. A bioassay was developed by extracting 20 g of compost of different maturities with various volumes of water, then measuring germination percentage of ivyleaf morningglory (Ipomoea hederacea) seeds placed on extract-saturated filter paper in a petri dish. A 20 g (dry weight) compost: 50 mL of water generated an extract that produced the widest percentage seed germination variation in response to composts of different maturity. Ivyleaf morningglory, barnyardgrass (Echinochloa crus-galli L.), purslane (Potulaca oleracea L.), and corn (Zea mays L) were selected as plant indicators to determine the compost maturity stage with maximum germination inhibition. Compost 8-week-old decreased percent germination, root growth, and germination index (combines germination rate and root growth), and increased mean days to germination (MDG) of each plant indicator. Immature 8 week-old compost extract effect on MDG and germination percent of 15 weed species was evaluated. Extract from 8-week-old compost inhibited germination in most weed species, except yellow nutsedge (Cyperus esculentus). Compost extracts derided from immature (3-day, 4-, and 8-week-old) compost resulted in delayed and reduced germination percent of important economic weed species.

Effect of straw extract on water absorption and germination of wheat (Triticum aestivum L. variety RR-21) seeds

Freshly prepared straw extract inhibited wheat seed germination by about 18 per cent but did not affect water absorption by germinating wheat seeds. The maximum germination inhibition (47 per cent) was noticed with extract of straw rotted for 15 days. The germination inhibitory effect of rotting straw was over at 31 days of straw rotting.