Maximal Voluntary Isometric Force Research Articles

Validity and reliability of the Valkyria Trainer Balance® portable force platform in its isometric mode

BACKGROUND: Isometric maximal voluntary force (IMVF) is essential for individuals’ health and physical performance. Therefore, there is a need for valid and reliable devices to assess IMVF. OBJECTIVE: To determine the validity and reliability of the Valkyria Trainer Balance® portable force platform in its isometric mode. METHODS: Fifty-eight physically healthy individuals (30 men and 28 women) participated in the study. A repeated measures design was used to compare the inter-day test-retest reliability of peak force. The validity of the Valkyria Trainer Balance® force platform was determined by comparing the peak force with the ArtOficio® force platform. The analysis consisted of the intraclass correlation coefficient (ICC), standard error of measurement (SEM), and coefficient of variation (CV). A CV ⩽ 10% and ICC ⩾ 0.80 were considered acceptable reliability, while a ⩽ 5% and ICC ⩾ 0.90 were regarded as high reliability. RESULTS: CV the peak force showed high test-retest inter-day reliability (CV = 4.3% and ICC = 0.99). When comparing both force platforms, there was a 1.1% difference between the two devices. CONCLUSIONS: The results of this study demonstrate that the Valkyria Trainer Balance® force platform is valid and reliable for assessing IMVF in physically healthy individuals.

Reliability and measurement error of a maximal voluntary toe plantarflexion measurement process

Despite the growing interest, information regarding the psychometric properties of maximal voluntary isometric toe plantarflexion force and rate of force development (RFD) is lacking. Hence, we investigate the test-retest reliability and measurement error of these outcome measurement instruments measured with a custom-built dynamometer. Twenty-six healthy adults participated in a crossed design with four sessions separated by 5–7 days. RFD was quantified using manual onset and calculating the impulse and the slope in the following time windows: 0–50 ms, 0–100 ms, 0–150 ms, 0–200 ms, 0–250 ms. We estimated the systematic bias of the mean, the intraclass correlation coefficient (ICC) and standard error of measurement (SEM) from the agreement and consistency models. The ICC and the SEM agreement for maximal voluntary isometric toe plantarflexion force along the perpendicular axis were respectively 0.87 (95%CI: 0.76, 0.93) and 27 N (22, 32), while along the resultant of the perpendicular and anterior posterior axis they were 0.85 (0.73, 0.92) and 29 N (23, 35). The results of the consistency model were similar as the estimated variance for session was closer to zero. A systematic bias of the mean between session 1 and 3 was found. For the RFD variables, the ICC agreement ranged from 0.35 to 0.65. The measurement process was found to be reliable to assess maximal voluntary isometric toe plantarflexion force but not RFD. However, a familiarization session is mandatory and these results need to be confirmed in less coordinated (e.g. aging population) individuals.

Sex differences in neuromuscular and biological determinants of isometric maximal force.

Force expression is characterized by an interplay of biological and molecular determinants that are expected to differentiate males and females in terms of maximal performance. These include muscle characteristics (muscle size, fiber type, contractility), neuromuscular regulation (central and peripheral factors of force expression), and individual genetic factors (miRNAs and gene/protein expression). This research aims to comprehensively assess these physiological variables and their role as determinants of maximal force difference between sexes. Experimental evaluations include neuromuscular components of isometric contraction, intrinsic muscle characteristics (proteins and fiber type), and some biomarkers associated with muscle function (circulating miRNAs and gut microbiome) in 12 young and healthy males and 12 females. Male strength superiority appears to stem primarily from muscle size while muscle fiber-type distribution plays a crucial role in contractile properties. Moderate-to-strong pooled correlations between these muscle parameters were established with specific circulating miRNAs, as well as muscle and plasma proteins. Muscle size is crucial in explaining the differences in maximal voluntary isometric force generation between males and females with similar fiber type distribution. Potential physiological mechanisms are seen from associations between maximal force, skeletal muscle contractile properties, and biological markers.

A comparison of the effects of sheep's milk and cow's milk on recovery from eccentric exercise.

When consumed after eccentric exercise, cow's milk has been shown to improve recovery and alleviate symptoms of exercise induced muscle damage. Although currently less commercially available than cow's milk, sheep's milk may offer similar or greater benefits for recovery as it is higher in protein and energy; however, the effect of sheep's milk in any exercise context has not been explored. This study compared the effects of a sheep's milk beverage and a cow's milk beverage on recovery from strenuous eccentric exercise. Additionally, the effects of each beverage on satiety and gastrointestinal comfort were assessed. Ten healthy males completed baseline measures of perceived muscle soreness and maximal voluntary concentric, eccentric, and isometric quadriceps force of one leg before completing 200 maximal eccentric knee extensions on an isokinetic dynamometer. Measures were repeated 0.5, 24, 48 and 72 h post-eccentric exercise. After 0.5 h measures, participants consumed either 450 ml of chocolate flavored sheep's milk or chocolate flavored cow's milk. Following a washout period, participants completed a second trial on the contralateral leg and consumed the other beverage. Additionally, a satiety and gastrointestinal comfort questionnaire was completed before and after each beverage was consumed. Eccentric exercise brought about a significant decrease in muscle function over time (all P < 0.012). No difference between treatments (all P > 0.097) was found. Measures of muscle soreness increased over time (all P < 0.002), however no difference was observed between treatments (all P > 0.072). Only sheep's milk altered perceived satiety, however, only the response to "How full do you feel" differed between treatments (P = 0.04). The results of this study suggest that consuming sheep's milk may provide similar benefits as cow's milk when recovering from exercise-induced muscle damage. While these findings provide initial support for the use of sheep's milk in a muscle recovery context, further research is warranted to confirm these findings. Given its superior nutritional profile, greater impact on satiety and lower environment impact, sheep's milk may be a more efficient post-exercise recovery beverage, compared to cow's milk.

Downhill running increases markers of muscle damage and impairs the maximal voluntary force production as well as the late phase of the rate of voluntary force development.

To examined the time-course of the early and late phase of the rate of voluntary force development (RVFD) and muscle damage markers after downhill running. Ten recreational runners performed a 30-min downhill run at 10kmh-1 and -20% (-11.3°) on a motorized treadmill. At baseline and each day up to 4days RVFD, knee extensors maximum voluntary isometric force (MVIC), serum creatine kinase (CK) concentration, quadriceps swelling, and soreness were assessed. The early (0-50ms) and late (100-200ms) phase of the RVFD, as well as the force developed at 50 and 200ms, were also determined. MVIC showed moderate decrements (p < 0.05) and recovered after 4days (p > 0.05). Force at 50ms and the early phase were not impaired (p > 0.05). Conversely, force at 200ms and the late phase showed moderate decrements (p < 0.05) and recovered after 3 and 4days, respectively (p > 0.05). CK concentration, quadriceps swelling, and soreness increased (p < 0.05) were overall fully resolved after 4days (p > 0.05). Downhill running affected the knee extensors RVFD late but not early phase. The RVFD late phase may be used as an additional marker of muscle damage in trail running.

Single-session measures of quadriceps neuromuscular function are reliable in healthy females and unaffected by age.

This study aimed to determine the inter-session reliability of quadriceps neuromuscular function measurements in healthy young and older females. Twenty-six females aged 19-74 years completed two identical experimental sessions on different days. Quadriceps neuromuscular function measurements included isometric maximal voluntary force, high- and low-frequency twitch force, voluntary and evoked(H-reflex, M-wave) electromyography (EMG), and estimatedmaximal torque, velocity and power derived from torque-velocity relationships. Intra-class correlation coefficients (ICCs), coefficients of variation (CoV) and Bland-Altman plots assessed inter-session reliability. The effect of age on reliability was assessed by linear regression. Excellent reliability (ICC > 0.8) was shown for all voluntary and evoked mechanical outcomes. Vastus lateralis EMG outcomes showed excellent reliability (ICC > 0.8) with CoVs < 12%, which were better than those of vastus medialis and rectus femoris. Age was not associated with reliability for 27/28 outcomes (P > 0.05). Excellent reliability of voluntary and evoked force and vastus lateralis EMG outcomes measured in healthy females can be attained in one experimental session, irrespective of age. Female neuromuscular function can be accurately assessed across the lifespan with minimal inconvenience, increasing feasibility for future research. The random error should however be considered when quantifying age-related differences in neuromuscular function.

Predicting muscle fatigue during dynamic contractions using wavelet analysis of surface electromyography signal

Muscle fatigue is defined as a reduction in the capability of muscle to exert force or power. Although surface electromyography (sEMG) signals during exercise have been used to assess muscle fatigue, analyzing the sEMG signal during dynamic contractions is difficult because of the many signal distorting factors such as electrode movements, and variations in muscle tissue conductivity. Besides the non-deterministic and non-stationary nature of sEMG in dynamic contractions, no fatigue indicator is available to predict the ability of a muscle to apply force based on the sEMG signal properties.In this study, we designed and manufactured a novel wearable sensor system with both sEMG electrodes and motion tracking sensors to monitor the dynamic muscle movements of human subjects. We detected the state of muscle fatigue using a new wavelet analysis method to predict the maximum isometric force the subject can apply during dynamic contraction.Our method of signal processing consists of four main steps. 1- Segmenting sEMG signals using motion tracking signals. 2- Determine the most suitable mother wavelet for discrete wavelet transformation (DWT) based on cross-correlation between wavelets and signals. 3- Deoinsing the sEMG using the DWT method. 4- Calculation of normalized energy in different decomposition levels to predict maximal voluntary isometric contraction force as an indicator of muscle fatigue.The monitoring system was tested on healthy adults doing biceps curl exercises, and the results of the wavelet decomposition method were compared to well-known muscle fatigue indices in the literature.

Feasibility and early effects of bed-cycling eccentric training: Potential clinical applications.

This study aimed to evaluate feasibility and early effects of moderate intensity bed-cycling eccentric training on healthy individuals, and establish whether this training modality could be implemented into bedridden patients'routine care. Longitudinal study with prepost exercise intervention measurements. The development of a bed-adapted eccentric ergometer allowed to conduct five training sessions during 3 weeks at increasing intensity on 11 healthy individuals. Force-speed relationship, maximal voluntary knee extension force and neural activation of subjects were evaluated before and after the programme. Five training sessions were sufficient to decrease the rate of perceived exertion whereas eccentric power output increased (+40%). After training, maximal voluntary isometric contraction force measured during knee extension had significantly improved in all subjects, with a mean increase of 17%. Maximal cycling power was also significantly higher (+7%) after the training programme. Taken together, these results show that moderate load eccentric bed cycling (i) was feasible and efficient, (ii) did not generate excessive individual perception of effort during exercise nor develop major muscular or joint pain after training and (iii) allowed early force and power gains in healthy subjects.

Load-Specific Performance Fatigability, Coactivation, and Neuromuscular Responses to Fatiguing Forearm Flexion Muscle Actions in Women.

Benitez, B, Dinyer-McNeeley, TK, McCallum, L, Kwak, M, Succi, PJ, and Bergstrom, HC. Load-specific performance fatigability, coactivation, and neuromuscular responses to fatiguing forearm flexion muscle actions in women. J Strength Cond Res 37(4): 769-779, 2023-This study examined the effects of fatiguing, bilateral, dynamic constant external resistance (DCER) forearm flexion on performance fatigability, coactivation, and neuromuscular responses of the biceps brachii (BB) and triceps brachii (TB) at high (80% 1 repetition maximum [1RM]) and low (30% 1RM) relative loads in women. Ten women completed 1RM testing and repetitions to failure (RTF) at 30 and 80% 1RM. Maximal voluntary isometric force was measured before and after RTF. Electromyographic (EMG) and mechanomyographic (MMG) amplitude (AMP) and mean power frequency (MPF) signals were measured from the BB and TB. Performance fatigability was greater (p < 0.05) after RTF at 30% (%∆ = 41.56 ± 18.61%) than 80% (%∆ = 19.65 ± 8.47%) 1RM. There was an increase in the coactivation ratio (less coactivation) between the initial and final repetitions at 30%, which may reflect greater increases in agonist muscle excitation (EMG AMP) relative to the antagonist for RTF at 30% than 80% 1RM. The initial repetitions EMG AMP was greater for 80% than 30% 1RM, but there was no difference between loads for the final repetitions. For both loads, there were increases in EMG MPF and MMG AMP and decreases in MMG MPF that may suggest fatigue-dependent recruitment of higher-threshold motor units. Thus, RTF at 30 and 80% 1RM during DCER forearm flexion may not necessitate additional muscle excitation to the antagonist muscle despite greater fatigability after RTF at 30% 1RM. These specific acute performance and neuromuscular responses may provide insight into the unique mechanism underlying adaptations to training performed at varying relative loads.

Effects of Blood Flow Restricted Exercise on Electromechanical Delay and Time to Peak Force after Task Failure: A Randomized Crossover Trial

Introduction: Electromechanical delay (ED) and time to peak force (TPF) could be used to investigate the central or peripheral sources of performance decline in fatiguing tasks. Exercise with partial blood flow restriction (BFR) has been shown to induce fatigue, but the repercussions of exercise with partial BFR on ED and TPF are unclear. The present study aimed to compare the ED and TPF after an intermittent isometric task until failure with BFR and free blood flow (FBF). Methods: In this crossover randomized clinical trial, 15 healthy and physically active men volunteered in this study. Volunteers performed two intermittent isometric handgrip exercise (IIHE) to failure (72 h apart), combined with either BFR or FBF. Maximum voluntary isometric force (MVIF) concomitant with the electromyographic activity of the wrist and finger flexor muscles were assessed before (PRE) and one minute after (POST) the task failure. Within (PRE vs. POST) and between comparisons (eFBF vs. eBFR) of peak force, time to peak force, rate of force development (RFDpeak) and ED were carried out. Results: No significant between-intervention differences were identified pre- or post-exercise. Peak force and RFDpeak reduced significantly after both blood flow conditions (p &lt; 0.05), but without between-condition difference. TPF was statistically higher after exercise only in the FBF intervention (p &lt; 0.05). None of the interventions induced a significant change in the ED after IIHE. Conclusion: ED and TPF were similar after BFR and FBF, indicating both conditions induce similar acute performance impairments after IIHE, which seems not to be caused by local (i.e., muscular) factors, but probably by central (i.e., neural) factors.

Knee position sense and knee flexor neuromuscular function are similarly altered after two submaximal eccentric bouts.

This study examined eccentric-induced fatigue effects on knee flexor (KF) neuromuscular function and on knee position sense. This design was repeated across two experimental sessions performed 1week apart to investigate potential repeated bout effects. Sixteen participants performed two submaximal bouts of KF unilateral eccentric contractions until reaching a 20% decrease in maximal voluntary isometric contraction force. Knee position sense was evaluated with position-matching tasks in seated and prone positions at 40° and 70° of knee flexion so that KF were either antagonistic or agonistic during the positioning movement. The twitch interpolation technique was used to assess KF neuromuscular fatigue. Perceived muscle soreness was also assessed. Measurements were performed before, immediately (POST) and 24h after (POST24) each eccentric bout. No repeated bout effect on neuromuscular function and proprioceptive parameters was observed. At POST, central and peripheral factors contributed to the force decrement as shown by significant decreases in voluntary activation level (-3.8 ± 4.8%, p < 0.01) and potentiated doublet torque at 100Hz (-10 ± 15.8%, p < 0.01). At this time point, position-matching errors significantly increased by 1.7 ± 1.9° in seated position at 40° (p < 0.01). At POST24, in presence of muscle soreness (p < 0.05), although KF neuromuscular function had recovered, position-matching errors increased by 0.6 ± 2.6° in prone position at 40° (p < 0.01). These results provide evidence that eccentric-induced position sense alterations may arise from central and/or peripheral mechanisms depending on the testing position.

Comparison between Eccentric-Only and Coupled Concentric-Eccentric Contractions for Neuromuscular Fatigue and Muscle Damage.

Eccentric contractions induce muscle damage, but less is known about the effects of preceding concentric contractions to eccentric contractions on muscle damage. We compared eccentric-only (ECC) and coupled concentric and eccentric contractions (CON-ECC) of the knee extensors for parameters of neuromuscular fatigue and muscle damage. Twenty participants (age, 19-36 yr) were randomly placed into an ECC or a CON-ECC group (n = 10 per group), without significant (P > 0.06) differences in baseline neuromuscular variables between groups. The ECC group performed six sets of eight ECC at 80% of ECC one-repetition maximum (1-RMecc), whereas the CON-ECC group performed six sets of eight alternating concentric (CON) and ECC (16 contractions per set) at 80% of CON 1-RM and 1-RMecc, respectively. Maximal voluntary isometric contraction force, rate of force development, resting twitch force, maximal M-wave (MMAX), voluntary activation, motor evoked potentials, corticospinal silent period, short interval intracortical inhibition, and muscle soreness were measured before, immediately after, and 1-3 d after exercise. No significant (P ≥ 0.09) differences between ECC and CON-ECC were observed for changes in any variables after exercise. However, maximal voluntary isometric contraction force decreased immediately after exercise (ECC: -20.7% ± 12.8%, CON-ECC: -23.6% ± 23.3%) and was still reduced 3 d after exercise (ECC: -13.6% ± 13.4%, CON-ECC: -3.3% ± 21.2%). Rate of force development at 0-30 ms reduced immediately after exercise (ECC: -38.3% ± 33.9%, CON-ECC: -30.7% ± 38.3%). Voluntary activation, resting twitch force, and motor evoked potential/MMAX decreased and corticospinal silent period increased after exercise (all P ≤ 0.03), but short interval intracortical inhibition and MMAX did not change. Muscle soreness developed (P < 0.001) similarly for both groups (peak, 38.5 ± 29.5 mm). CON-ECC did not exacerbate neuromuscular fatigue and muscle damage when compared with ECC, despite twice as many contractions performed. Thus, eccentric contractions (n = 48 in both groups) seemed to mainly mediate the neuromuscular responses observed.

Perceived Muscle Soreness Does Not Modulate Corticospinal Excitability

Soreness due to exercise-induced muscle damage temporarily impairs motor performance. Transcranial magnetic stimulation (TMS) is often used to assess subtle changes in corticospinal excitability (CSE) and motor system input-output properties and demonstrates sensitivity to chronic pain. Nevertheless, the acute effects of muscle soreness on TMS-based measures of corticospinal excitability require further investigation. PURPOSE: To examine the relationship between perceived muscle soreness and CSE of postural and lower-extremity muscles. METHODS: 12 healthy adults (3 W, age: 27.2 ± 5.4 yr, ht: 175.6 ± 11.0 cm, wt: 72.6 ± 13.4 kg) completed three visits with maximal anaerobic lower extremity exercise. At the start of each visit, participants confirmed they abstained from any physical activity 24 hr prior, and rated their overall residual muscle soreness using a 100 mm visual analog scale. Visits were ranked based on muscle soreness with the highest and lowest soreness visits retained for analysis. To assess CSE, electromyographic motor-evoked potentials (MEPs) were recorded from the right rectus abdominis (RA) and vastus lateralis (VL). Active motor thresholds (AMT) were determined for each muscle during 15% of maximal voluntary isometric force using a biphasic stimulator and 96 mm curved double coil. To determine CSE, ten TMS pulses were applied to each M1 hotspot at 120% AMT with the resultant MEPs averaged based on peak-to-peak amplitude from 15-65 ms post-stimulus. Paired-samples t-test were used to assess mean differences in soreness, AMT, and MEP amplitudes. RESULTS: As expected, muscle soreness differed between visits (mean difference: 25.3 mm, t = 5.08, p < 0.01). Similar motor-thresholds were observed for the RA (mean difference: 0.3%, t = 0.26, p = 0.80) and VL (mean difference: 0.5%, t = 0.45, p = 0.66). MEP amplitudes were also similar for the RA (mean difference: 0.13 mV, t = 1.74, p = 0.12) and VL (mean difference: 0.01 mV, t = 0.30, p = 0.77). CONCLUSION: Day-to-day variation in CSE during lower extremity and axial contractions is not explained by differences in muscle soreness. Future work should examine CSE at multiple timepoints after acute exercise and use various submaximal intensities to further clarify whether CSE is responsive to muscle soreness.

Resistance exercise with different workloads have distinct effects on cellular respiration of peripheral blood mononuclear cells.

Little is known how acute exercise‐induced inflammation and metabolic stress affect immune cell bioenergetics and the portion of its components. Therefore, we investigated acute effects of eccentric‐only (E), concentric‐only (C) and combined eccentric‐concentric resistance exercise (E + C) bouts on cellular respiration of peripheral blood mononuclear cells (PBMCs). Twelve strength‐trained young men performed bench press resistance exercises in randomized order. Venous blood samples were drawn at pre‐, 5 min post‐ and 24 h post‐exercise. Several PBMC respiration states were measured using high‐resolution respirometry. Levels of leukocytes, interleukin 6 (IL‐6), C‐reactive protein (CRP), creatine kinase (CK), blood lactate and maximum voluntary isometric force were measured from the same time points. Effects of blood lactate and pH change on bioenergetics of PBMCs were investigated ex vivo. PBMC routine respiration (p = 0.017), free routine capacity (p = 0.025) and ET‐capacity (p = 0.038) decreased immediately after E + C. E responded in opposite manner 5 min post‐exercise compared to E + C (p = 0.013) and C (p = 0.032) in routine respiration, and to E + C in free routine activity (p = 0.013). E + C > C > E was observed for increased lactate levels and decreased isometric force that correlated with routine respiration (R = −0.369, p = 0.035; R = 0.352, p = 0.048). Lactate and pH change did not affect bioenergetics of PBMCs. Acute resistance exercise affected cellular respiration of PBMCs, with training volume and the amount of metabolic stress appear influential. Results suggest that acute inflammation response does not contribute to changes seen in cellular respiration, but the level of peripheral muscle fatigue and metabolic stress could be explaining factors.

Modulation of subjective peripheral sensation, F-waves, and somatosensory evoked potentials in response to unilateral pinch task measured on the contractile and non-contractile sides.

Depression of the sensory input during voluntary muscle contractions has been demonstrated using electrophysiological methods in both animals and humans. However, the association between electrophysiological responses of the sensory system and subjective peripheral sensation (SPS) during a voluntary muscle contraction remains unclear. This study aimed to describe the changes in SPS, spinal α-motoneuron excitability (F-wave to M-wave amplitude), and somatosensory evoked potentials (SEPs) during a unilateral pinch-grip task. Outcome variables were measured on the side ipsilateral and contralateral to the muscle contraction and at rest (control). Participants were 8 healthy men aged 20.9±0.8 years. The isometric pinch-grip task was performed at 30% of the maximum voluntary isometric force measured for the right and left hands separately. The appearance rate of the F-wave during the task was significantly higher for the ipsilateral (right) hand than for the contralateral (left) hand and control condition. Although there was no difference in the F-wave latency between hands and the control condition, the amplitude of the F-wave was significantly higher for the ipsilateral (right) hand than for the contralateral (left) hand and the control condition. There was no difference in the amplitude of the SEP at N20. However, the amplitude at P25 was significantly lower for the ipsilateral (right) hand than for the contralateral (left) hand and the control condition. The accuracy rate of detecting tactile stimulation, evaluated for 20 repetitions using a Semmes–Weinstein monofilament at the sensory threshold for each participant, was significantly lower during the pinch-grip task for both the ipsilateral (right) and contralateral (left) hands than in the control condition. Overall, our findings show that SPS and neurophysiological parameters were not modulated in parallel during the task, with changes in the subjective sensation preceding changes in electrophysiological indices during the motor task. Our findings provide basic information on sensory-motor coordination.

Mitochondria-targeted antioxidant supplementation does not affect muscle soreness or recovery of maximal voluntary isometric contraction force following muscle-damaging exercise in untrained men: a randomized clinical trial.

Unaccustomed exercise causes muscle damage resulting in loss of muscle function, which may be attributable to exercise-induced increases in skeletal muscle reactive oxygen species. This study examined the effect of mitochondria-targeted antioxidant supplementation on recovery of muscle function following exercise. Thirty-two untrained men received MitoQ (20mg/day) or a placebo for 14 days before performing 300 maximal eccentric contractions of the knee extensor muscles of 1 leg. Muscle function was assessed using isokinetic dynamometry before, immediately after, and 24, 48, 72, and 168 hours after exercise. Muscle soreness was assessed using a visual analogue scale 24, 48, 72, and 168 hours after exercise. Blood samples were collected before, immediately after, and 2, 24, 48, 72, and 168 hours after exercise and urine samples were collected before and during the 48 hours after exercise. The reduction in maximal voluntary isometric contraction force and peak concentric torque following exercise was unaffected by MitoQ while recovery of peak eccentric torque was delayed in the MitoQ group. Exercise-induced increases in urine F2-isoprostanes were unaffected by MitoQ. MitoQ augmented exercise-induced increases in plasma creatine kinase levels, while plasma IL-6 was similar between groups. Muscle soreness was not affected by MitoQ. These results indicate that MitoQ does not attenuate post-exercise muscle soreness and may delay recovery of muscle function following eccentric exercise. Trial registration number: ACTRN12620001089921. Novelty: Post-exercise recovery of maximal voluntary isometric contraction force and peak concentric torque were unaffected by MitoQ. MitoQ delayed post-exercise recovery of peak eccentric torque. Post-exercise muscle soreness was unaffected by MitoQ.

Muscle volume vs. anatomical cross-sectional area: Different muscle assessment does not affect the muscle size-strength relationship

Muscle volume (MV) and anatomical cross-sectional area (CSA) are used as measures of muscle-size, but determining these from magnetic resonance imaging (MRI) is a very time-consuming process. Additionally, it is unclear if the use of different muscle size assessments (all vs. reduced number of slices images) would impact the muscle size-strength relationship. Thus, this study aimed to investigate if muscle size calculation by using a reduced or all slices images from pectoralis major (PM) would maintain a similar muscle size-strength relationship with bilateral maximal dynamic and isometric contractions on a bench press exercise. Twenty-four healthy males underwent an MRI examination to measure PM muscle size, and maximal isometric and dynamic contractions (by one repetition maximum, 1RM) were performed. Correlations between maximal isometric voluntary force (MVF) and dynamic strength (1RM) with muscle size variables [three images from the largest part of PM (CSA3MAX), three images accounting for the shape -first image, middle image, final image- of the PM (CSA3), and MV] were performed. The correlation between 1RM with MV, CSA3, and CSA3MAX were 0.84, 0.832, and 0.727 (p < 0.001), respectively. The correlation between MVF with MV, CSA3, and CSA3MAX were 0.738, 0.733, and 0.604 (p < 0.001), respectively. Overall, PM MV and CSA3 exhibit a stronger and similar muscle size-strength relationship during maximal dynamic and isometric tests than CSA3MAX. Therefore, a reduced number of slices (CSA3) could be used as an alternative to considerably reduce the time of analysis without compromise muscle size-strength relationship.

Deficit in knee extension strength following anterior cruciate ligament reconstruction is explained by a reduced neural drive to the vasti muscles.

The persistence of quadriceps weakness represents a major concern following anterior cruciate ligament reconstruction (ACLR). The underlying adaptations occurring in the activity of spinal motoneurons are still unexplored. This study examined the discharge patterns of large populations of motor units (MUs) in the vastus lateralis (VL) and vastus medialis muscles following ACLR. Nine ACLR individuals and 10 controls performed unilateral trapezoidal contractions of the knee extensor muscles at 35%, 50% and 70% of the maximal voluntary isometric force (MVIF). High-density surface electromyography (HDsEMG) was used to record the myoelectrical activity of the vasti muscles in both limbs. HDsEMG signals were decomposed with a convolutive blind source separation method and MU properties were extracted and compared between sides and groups. The ACLR group showed a lower MVIF on the reconstructed side compared to the contralateral side (28.1%; P<0.001). This force deficit was accompanied by reduced MU discharge rates (∼21%; P<0.05), lower absolute MU recruitment and derecruitment thresholds (∼22% and ∼22.5%, respectively; P<0.05) and lower input-output gain of motoneurons (27.3%; P=0.009). Deficits in MU discharge rates of the VL and in absolute recruitment and derecruitment thresholds of both vasti MUs were associated with deficits in MVIF (P<0.05). A strong between-side correlation was found for MU discharge rates of the VL of ACLR individuals (P<0.01). There were no significant between-group differences (P>0.05). These results indicate that mid- to long-term strength deficits following ACLR may be attributable to a reduced neural drive to vasti muscles, with potential changes in excitatory and inhibitory synaptic inputs. KEY POINTS: Impaired expression and control of knee extension forces is common after anterior cruciate ligament reconstruction and is related to high risk of a second injury. To provide novel insights into the neural basis of this impairment, the discharge patterns of motor units in the vastus lateralis and vastus medialis were investigated during voluntary force contractions. There was lower knee extensor strength on the reconstructed side with respect to the contralateral side, which was explained by deficits in motor unit discharge rate and an altered motoneuronal input-output gain. Insufficient excitatory inputs to motoneurons and increased inhibitory afferent signals potentially contributed to these alterations. These results further our understanding of the neural underpinnings of quadriceps weakness following anterior cruciate ligament reconstruction and can help to develop effective rehabilitation protocols to regain muscle strength and reduce the risk of a second injury.

Development and reliability of a new system for bedside evaluation of non-volitional knee extension force

PurposeNeuromuscular electrical stimulation (NMES) is a widely-used technique for diagnostic and therapeutic purposes. Here we developed and tested the reliability of a new NMES-dynamometer system for bedside evaluation of knee extensor muscle function. Materials and methodsThirty-two healthy participants (16 men, 16 women; 27±5 years) completed two testing sessions, 7 days apart. On day 1, a single experienced rater, who repeated the evaluation on day 2 with two other raters, completed a standardized testing procedure. Participants were placed supine, with knees flexed and legs connected to the dynamometer. Maximal voluntary knee extensor isometric force (MVF) and supramaximal twitch force (TwF) were obtained. ResultsHigh intra-rater intraclass correlation coefficients were observed for both MVF (0.91) and TwF (0.94). MVF and TwF standard error of measurements (8.2%, 5.9%) and minimal detectable changes (16%, 11.6%) were low compared to mean values. High intraclass correlation coefficients were also observed for inter-rater comparisons of MVF (0.89) and TwF (0.86). Standard errors of measurements (MVF: 8.7%, TwF: 5.5%) and minimal detectable changes (MVF: 17.2%, TwF: 10.8%) were similar to intra-rater comparisons. ConclusionThe good reliability of the novel NMES-dynamometer system suggests it as an appropriate tool for the bedside evaluation of knee extensor muscle function.

Endurance training-induced increase in muscle oxidative capacity without loss of muscle mass in younger and older resistance-trained men

While concurrent training is regularly used in older populations, the inverse relationship between fibre size and oxidative capacity suggests that endurance training in resistance-trained individuals may result in some loss of resistance training-induced gains in muscle mass, which may be more pronounced in older people. We investigated the impact of superimposed endurance training in younger (28.5 ± 4.8 years; n = 8) and older (67.5 ± 5.5 years; n = 7) highly resistance-trained men. Participants underwent a 10-week endurance cycling training programme consisting of five 6-min intervals at 75% max heart rate (HRmax) separated by 4-min intervals at 90% HRmax. The anatomical cross-sectional area (ACSA) of the thigh muscles, as determined with MRI, was 24% smaller in older compared to younger participants (p < 0.001). Although maximal oxygen consumption (VO2max) was also lower in the older group (p < 0.001), VO2max per kg body mass did not differ significantly between younger and older participants. Histological analyses of biopsies of the m. vastus lateralis showed that endurance training induced an increase in succinate dehydrogenase activity in both younger and older participants (p ≤ 0.043), and an increase in the number of capillaries around type I fibres (p = 0.017). The superimposed endurance training did not induce a significant decrease in thigh ACSA, fibre cross-sectional area, or knee extensor maximum voluntary isometric force. These observations indicate that adding endurance training to resistance training can lead to positive endurance-related adaptations without negative consequences for muscle size and strength in older and younger resistance-trained people.