MATH Domain Research Articles

Genome-wide identification of the TRAF gene family in humpback grouper (Cromileptes altivelis) and analysis of their expression in response to Vibrio harveyi challenge

TRAF (Tumor necrosis factor receptor-associated factor) proteins are key mediators of signal transduction in cell signaling and immune regulation within the toll-like receptor (TLR) and tumor necrosis factor (TNFR) superfamily. Despite the importance of TRAF genes in teleost innate immunity, study on their functions in C. altivelis is limited. This study utilized bioinformatics methods to identify and named eight TRAF genes (CaTRAF2a, CaTRAF2a-like, CaTRAF2b, CaTRAF3, CaTRAF4a, CaTRAF5, CaTRAF6 and CaTRAF7) in C. altivelis. Phylogenetic, syntenic and molecular evolution revealed that all CaTRAF members were evolutionarily conserved in teleost. Domain analysis indicated the presence of a conserved N-terminal RING finger domain in all CaTRAF proteins. Most CaTRAF proteins also featured a MATH domain at the C-terminal, with the exception of CaTRAF7 which contained seven repeat WD40 domains. In addition, qRT-PCR was used to detect the expression patterns of nine different tissues and eight different embryonic development stages of healthy fish, and it was found that there were spatial and tissue specificities among the members. HE staining revealed evident pathological lesions in the tissues post V. harveyi infection. Atrophy and significant bending of the gill lamellae were observed in the gills, while irregular cell shapes, increased fat vacuoles, and enlarged cell volume were noted in the liver. Intestinal tissues displayed thickening of the muscle layer, elongation of intestinal villi, and increased folds. Moreover, the expression of TRAF gene changed significantly after V. harveyi infection. These results would help to clarify the molecular role of CaTRAF gene in the regulation of immune and inflammatory responses in C. altivelis.

Abstract 1992: A novel RB1 E3 ligase TRIM37 contributes to palbociclib resistance in breast cancer

Abstract The tripartite motif-containing 37 (TRIM37) is a RING finger E3 ubiquitin ligase and is a poor prognostic factor in breast cancer patients. The gene is located in chromosome 17q23, a region amplified in up to 40% of breast cancers. We observed that activation of androgen receptor (AR) could induce resistance to the CDK4/6 inhibitor Palbociclib, the mechanism yet to be clarified. ChIP-sequencing data suggested that AR binds to the TRIM37 promoter, with AR activation resulting in TRIM37 expression. We hypothesized that TRIM37 might be involved in Palbociclib resistance. Using an ER+ve cell line MCF7 and a TNBC cell line MDA-MB 453, we found that overexpression of TRIM37 could induce Palbociclib resistance. TRIM37 overexpression resulted in RB1 protein downregulation, which is the primary downstream target of CDK4/6, while TRIM37 knockdown enhanced RB1 protein expression. Ubiquitination assay demonstrated TRIM37 overexpression increased the level of RB1 protein ubiquitination. The co-immunoprecipitation (Co-IP) assay confirmed that TRIM37 protein could interact with the RB1 protein. These results suggest that TRIM37 could be the E3 ligase that regulates RB1 degradation. Various deletion mutants of TRIM37 were cloned for Co-IP experiments between these mutants and RB1. Results show that the MATH and c-terminal domains of TRIM37 was the essential region for the interaction. Overexpression of the mutant with the TRIM37 MATH and c-terminal domains (TRIM37-MATH/C) could enhance RB1 protein level. This might be due to competition of TRIM37-MATH/C with wild-type TRIM37 for RB1 binding. Our findings suggest TRIM37 is a novel RB1 E3 ligase, and it binds to the RB1 protein via the MATH and C-terminal domains to regulate protein degradation. Our study also suggests that high expression of TRIM37 mediated by AR activation could deplete RB1, which is the target of Palbociclib, eventually leading to resistance. These findings are supportive of the contribution of AR activation to Palbociclib resistance. Citation Format: Chanping You, Ho Tsoi, Pui Sum Man, Larry Ka Yue Chow, Wei Dai, Ui Soon Khoo. A novel RB1 E3 ligase TRIM37 contributes to palbociclib resistance in breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1992.

SPOP point mutations regulate substrate preference and affect its function

The adaptor SPOP recruits substrates to CUL3 E3 ligase for ubiquitination and degradation. Structurally, SPOP harbors a MATH domain for substrate recognition, and a BTB domain responsible for binding CUL3. Reported point mutations always occur in SPOP’s MATH domain and are through to disrupt affinities of SPOP to substrates, thereby leading to tumorigenesis. In this study, we identify the tumor suppressor IRF2BP2 as a novel substrate of SPOP. SPOP enables to attenuate IRF2BP2-inhibited cell proliferation and metastasis in HCC cells. However, overexpression of wild-type SPOP alone suppresses HCC cell proliferation and metastasis. In addition, a HCC-derived mutant, SPOP-M35L, shows an increased affinity to IRF2BP2 in comparison with wild-type SPOP. SPOP-M35L promotes HCC cell proliferation and metastasis, suggesting that M35L mutation possibly reprograms SPOP from a tumor suppressor to an oncoprotein. Taken together, this study uncovers mutations in SPOP’s MATH lead to distinct functional consequences in context-dependent manners, rather than simply disrupting its interactions with substrates, raising a noteworthy concern that we should be prudent to select SPOP as therapeutic target for cancers.

Addressing the Binding Mechanism of the Meprin and TRAF-C Homology Domain of the Speckle-Type POZ Protein Using Protein Engineering.

Protein-protein interactions play crucial roles in a wide range of biological processes, including metabolic pathways, cell cycle progression, signal transduction, and the proteasomal system. For PPIs to fulfill their biological functions, they require the specific recognition of a multitude of interacting partners. In many cases, however, protein-protein interaction domains are capable of binding different partners in the intracellular environment, but they require precise regulation of the binding events in order to exert their function properly and avoid misregulation of important molecular pathways. In this work, we focused on the MATH domain of the E3 Ligase adaptor protein SPOP in order to decipher the molecular features underlying its interaction with two different peptides that mimic its physiological partners: Puc and MacroH2A. By employing stopped-flow kinetic binding experiments, together with extensive site-directed mutagenesis, we addressed the roles of specific residues, some of which, although far from the binding site, govern these transient interactions. Our findings are compatible with a scenario in which the binding of the MATH domain with its substrate is characterized by a fine energetic network that regulates its interactions with different ligands. Results are briefly discussed in the context of previously existing work regarding the MATH domain.

Zebrafish spop promotes ubiquitination and degradation of mavs to suppress antiviral response via the lysosomal pathway

Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) signaling pathways are required to be tightly controlled to initiate host innate immune responses. Fish mitochondrial antiviral signaling (mavs) is a key determinant in the RLR pathway, and its ubiquitination is associated with mavs activation. Here, we identified the zebrafish E3 ubiquitin ligase Speckle-type BTB–POZ protein (spop) negatively regulates mavs-mediated the type I interferon (IFN) responses. Consistently, overexpression of zebrafish spop repressed the activity of IFN promoter and reduced host ifn transcription, whereas knockdown spop by small interfering RNA (siRNA) transfection had the opposite effects. Accordingly, overexpression of spop dampened the cellular antiviral responses triggered by spring viremia of carp virus (SVCV). A functional domain assay revealed that the N-terminal substrate-binding MATH domain regions of spop were necessary for IFN suppression. Further assays indicated that spop interacts with mavs through the C-terminal transmembrane (TM) domain of mavs. Moreover, zebrafish spop selectively promotes K48-linked polyubiquitination and degradation of mavs through the lysosomal pathway to suppress IFN expression. Our findings unearth a post-translational mechanism by which mavs is regulated and reveal a role for spop in inhibiting antiviral innate responses.

Non-native Accents among School Beginners and Teacher Expectations for Future Student Achievements

Based on sociological, economic, and social-psychological theories of discrimination and bias, this study addresses non-native accents among ethnic minority students as they begin school and explores effects of such accents on their teachers’ achievement expectations. Using a unique data set of first graders in Germany, the analysis reveals that a non-native accent is relevant to teachers’ expectations net of student skills, abilities, and other background variables. Associations are stronger in the language domain than in mathematics, indicating that teachers perceive accent-free speech as a language-learning requirement. However, residual influences of non-native accents on teacher expectations also exist in the math domain and persist even after prolonged periods of teacher-student interaction. Mechanisms of statistical discrimination and stereotype-based discrimination can partially explain these effects. However, the overall pattern of results suggests a stigmatization of non-native accents, potentially resulting from the activation of negative associations related to foreignness and disfluency.

Understanding Mathematics Perception through Drawing: An Online Interpretative Phenomenological Analysis (OIPA) Study

In this study, it is aimed to reveal the images formed in the minds of high school students about the concept of mathematics with the help of their own expressions and drawings. In this qualitative study, the core of 100 high school students’ experiences of math was examined with a holistic approach using Online Interpretative Phenomenological Analysis (OIPA). In order to obtain in-depth information about the concept of math from the participants, the purposeful sampling method was used. It was paid full attention to ensure that the participants were high school students. The data were obtained by using the OIPA form, which allows students to create stories and drawings about the concept of math. The data obtained were analyzed in three stages by the researcher and an expert psychologist. As a result of the analysis, it was seen that there were deficiencies in students’ mathematical literacy in situations such as understanding the role of mathematics in daily life and using it to solve real life problems. Additionally, the students expressed the concept of math using 18 different main themes, most of which contained negative strong emotions. Students associated math most frequently with the themes of life (44%) and difficult math language (38%). The students associated math least frequently with the themes of hate (5%) and unimportant (5%). On the other hand, students explained the math domain with individual factors (74%) the most and exosystem (15%) the least. In line with the results obtained in the study, a few recommendations were made.

Predictors, outcomes, and inter-domain connections of German and Math academic motivation profiles

The present study investigated the nature and similarity of academic motivation profiles toward German and Math among German elementary school students while accounting for the dual global and specific nature of academic motivation proposed by self-determination theory. The determinants (self-concept, sex) and outcomes (anxiety, academic achievement, effort) were investigated. Latent Markov analyses revealed five profiles (Highly Extrinsic, Controlled, Moderately Extrinsic, Self-Determined, and Non-Motivated) characterized by differing levels of global and specific motivation. These profiles had similar properties (within-sample similarity) and had a similar membership (within-person similarity) across domains. Self-concept predicted membership into profiles with higher global self-determination, while girls were more likely to belong to the Highly Extrinsic profile. Most desirable outcomes were associated with the Self-Determined profile, then the Moderately Extrinsic profile, followed by the remaining three profiles. Educational relevance statementStudents' engagement in their academic work is seldom driven by a single motive but rather seems to be motivated for a variety of reasons. The present study identified naturally occurring motivational combinations for Math and German classes among a sample of elementary school students. Five profiles, characterized by differing levels of global and specific motivation levels, were identified which were replicated and had a similar membership across Math and German domains. We showed that students' sex and academic self-concepts are drivers of their motivation profile memberships, and that these profiles differ from one another in terms of youth's performance and well-being.

Molecular characterization and functional analysis of TRIM37 from black tiger shrimp (Penaeus monodon).

The family of TRIM proteins with E3 ubiquitin ligase activity served important roles in the regulation of innate immune processes, in particular antiviral and proinflammatory cytokine responses. In this study, a novel TRIM37 homolog was identified from Penaeus monodon (named PmTRIM37). The PmTRIM37 protein contained three conserved domains (one RING finger domain, a B-box, and one Coiled-coil region) at its N-terminal and one Meprin and MATH domain at its C-terminal. The MATH domain was the characteristic of TRIM37 family. PmTRIM37 has relatively high expression in immune-related tissues such as hepatopancreas, gills, lymphoid organs and hemocytes. The expression levels of PmTRIM37 in hepatopancreas and lymphoid organs were significantly up-regulated after white spot syndrome virus (WSSV) infection. Knock down of PmTRIM37 promoted WSSV replication and VP28 expression, suggesting that PmTRIM37 played a negative role in WSSV infection. Further studies revealed that PmTRIM37 positively regulated the NF-κB pathway and Antimicrobial peptides (AMP) expression during WSSV infection. These findings indicated that PmTRIM37 might restrict WSSV replication by positively regulating NF-κB pathway during WSSV infection in P. monodon.

The TRAF gene family in turbot (Scophthalmus maximus): Identification, characterization, molecular evolution and expression patterns analysis.

Tumor necrosis factor receptor-associated factor (TRAF) is an important structural protein, which can bind to TNF receptors and participate in the regulation of TNF signaling pathway. Nonetheless, few studies have been conducted to investigate the systematic identification of TRAF gene family in teleost and role in innate immunity of turbot (Scophthalmus maximus). In this study, eight TRAF genes, namely SmTRAF2aa, SmTRAF2ab, SmTRAF2b, SmTRAF3, SmTRAF4a, SmTRAF5, SmTRAF6 and SmTRAF7, were identified and annotated in turbot by using bioinformatics methods. Analysis of the phylogenetic, syntenic and molecular evolution demonstrated that all SmTRAF members were evolutionarily conserved in teleost. Domain analysis showed all SmTRAF proteins contained a typical conserved N-terminal RING finger domain. Most SmTRAF proteins contained a MATH domain at the C-terminal, while SmTRAF7 contains seven duplicate WD40 domains. In addition, quantitative real-time PCR was performed to detect the expression patterns of SmTRAFs in tissues from healthy and Vibrio anguillarum infected turbots. The results indicated SmTRAFs had diverse tissue expression patterns and the expression of TRAF gene changed significantly after V. anguillarum infection. This study provided a basis for understanding the roles of TRAFs in the innate immune response of turbot.

Cultural Adaptation of the Expectancy-Value-Cost Scale for Spanish-Speaking Students

The Expectancy-Value model has been extensively used to understand students' achievement motivation. However, recent studies propose the inclusion of cost as a separate construct from values, leading to the development of the Expectancy-Value-Cost model. This study aimed to adapt Kosovich et al.’s ( The Journal of Early Adolescence, 35, 5–6, 2015) instrument for assessing achievement motivation in science and math among Spanish-speaking students aged 9–12. The psychometric evaluation encompassed examining validity, reliability, and measurement invariance for gender. The findings support the applicability of the expectancy-value-cost model in elementary school students, providing a valid and reliable tool for assessing achievement motivation in the science and math domain. However, the findings for the measurement invariance for gender are less conclusive and warrant future psychometric evaluations. This study opens avenues for future research on achievement motivation in Spanish-speaking contexts and populations.

Social and dimensional comparison effects in general and domain-specific test anxiety: a nested factor modeling approach

The generalized internal/external frame of reference (GI/E) model assumes social and dimensional achievement comparisons to form self-perceptions. These domain-specific comparisons have been shown to shape two facets of test anxiety (i.e., worry and emotionality) both directly and indirectly through academic self-concepts. However, examinations of such domain-specific relations have rarely integrated general components, although the hierarchical nature of both test anxiety and academic self-concept is well-known. Thus, the present study implemented a nested factor modeling approach. We examined social and dimensional comparison effects on worry and emotionality as well as mediation effects of academic self-concepts in the math and verbal domains while controlling for general components. We contrasted this approach with the conventionally used first-order factor model where general components were not considered. Data from N = 348 German secondary school students (Mage = 15.3 years, Grades 9–10) were analyzed using structural equation models. Direct negative within-domain and positive cross-domain achievement-anxiety relations emerged, yet, the pattern of cross-domain relations changed across modeling approaches. Only the nested factor model showed indirect cross-domain mediation relations. Our findings suggest the importance of structural representations of hierarchical constructs. The nested factor model approach enhanced predictions within the GI/E model, particularly those related to dimensional comparisons.

Performance Evaluation and Optimization of the Weather Research and Forecasting (WRF) Model Based on Kunpeng 920

The Weather Research and Forecasting (WRF) model is a mesoscale numerical weather prediction system, which is widely used in major high-performance server platforms. This study focuses on the performance evaluation and optimization of WRF on Huawei’s self-developed kunpeng 920 processor platform, aiming to improve the operational efficiency of WRF. The results of the study show that the scalability of WRF on kunpeng 920 processor is well performed; the performance of WRF on kunpeng 920 processor is improved by 32.6% after invoking the Fast Math Library and Domain Decomposition Core Tile Division optimization. In terms of IO, the main optimizations are parallel IO and asynchronous IO. Eventually, the single output time of WRF is reduced from 37.28 s in serial IO mode to 0.14 s in asynchronous IO mode, and the overall running time is reduced from 1078.80 s to 807.94 s.

A COMPREHENSIVE REVIEW ON DATA STREAM MINING TECHNIQUES FOR DATA CLASSIFICATION; AND FUTURE TRENDS

Data Mining is a developing interdisciplinary control managing Data Reclamation and Data Stream Mining techniques, whose subject is gathering, overseeing, processing, breaking down, and visualizing the huge volume of organized or unstructured data. Data stream mining indicates how to look at Unknown patterns from a massive amount of data over algorithms. It has experienced quick improvement with significant progress in math, statistics, data science, and computer science domains. Data streams are commonly generated by various sources such as sensor networks, social media feeds, financial transactions, online retail, network traffic, and many other applications. The gathered data could be additionally utilized for various purposes, for example, execution assessment, irregularity discovery, change identification, or issue finding of the operating systems. This data stream analysis is done using different data stream mining techniques. This paper provides a broad overview of the distinct approaches used for data stream mining. Initially, we studied the different techniques of data stream mining. Next, we discuss the different clustering and classification techniques and their benefits. Then we examine the evaluation of different data stream mining techniques results that some techniques are feasible for real-time data streams and some of not. This study provides a complete understanding of techniques and their benefits. The studies done so far need to be sufficiently exhaustive for data mining techniques, so future work is needed to assess which technique is feasible for real-time data streams.

The big-fish-little-pond effect on academic self-concept and interest in first- and third-grade students

BackgroundThe big-fish-little-pond effect (BFLPE) postulates that class-average achievement has a negative effect on students’ academic self-concept. Research examining the BFLPE with elementary school students is scarce, especially with first graders. AimsThis study examined the BFLPE of class-average achievement on academic self-concept and interest in the math domain with first and third graders. SampleParticipants were Luxembourgish first graders (N = 5057) and third graders (N = 4925). MethodsA multilevel, doubly latent approach was used to assess a BFLPE model containing achievement (as the predictor) and ASC and interest (as outcomes) in the math domain. ResultsThe BFLPE on math self-concept was supported in both grades, whereas the BFLPE on math interest was supported only for third graders. In both grades, larger effect sizes were observed for the BFLPE on math self-concept than on math interest. ConclusionOur results suggest that the social comparisons underlying the BFLPE play an important role in the formation of math self-concept in both grades, but they play a less substantial—and probably later—role in the formation of math interest in elementary school.

Biophysical Characterization of the Binding Mechanism between the MATH Domain of SPOP and Its Physiological Partners.

SPOP (Speckle-type POZ protein) is an E3 ubiquitin ligase adaptor protein that mediates the ubiquitination of several substrates. Furthermore, SPOP is responsible for the regulation of both degradable and nondegradable polyubiquitination of a number of substrates with diverse biological functions. The recognition of SPOP and its physiological partners is mediated by two protein-protein interaction domains. Among them, the MATH domain recognizes different substrates, and it is critical for orchestrating diverse cellular pathways, being mutated in several human diseases. Despite its importance, the mechanism by which the MATH domain recognizes its physiological partners has escaped a detailed experimental characterization. In this work, we present a characterization of the binding mechanism of the MATH domain of SPOP with three peptides mimicking the phosphatase Puc, the chromatin component MacroH2A, and the dual-specificity phosphatase PTEN. Furthermore, by taking advantage of site-directed mutagenesis, we address the role of some key residues of MATH in the binding process. Our findings are briefly discussed in the context of previously existing data on the MATH domain.

Abstract 937: Opposing roles of SPOP mutations in human prostate and endometrial cancers

Abstract Recurrent mutations in the gene encoding the Speckle Type POZ Protein, SPOP, the substrate-binding components of E3-ubiquitin ligase, have been identified as common alterations in prostate cancer (PCa) and endometrial cancer (EC). SPOP mutations are associated with PCa and EC growth and progression. Here, we characterized SPOP mutations in PCa and EC, and their association with molecular and immune signatures. 7,398 PCa and 19,188 EC samples were analyzed by next-generation sequencing (592, NextSeq; WES, NovaSeq), (WTS; NovaSeq) (Caris Life Sciences, Phoenix, AZ). Real world overall survival (rwOS) was extracted from insurance claims and depicted using Kaplan-Meier survival curves for molecularly defined cohorts from tissue collection to last contact. Statistical significance was determined using chi-square and Mann-Whitney U tests, with p-values adjusted for multiple comparisons (q<0.05). SPOP mutations were identified in 601 (8.1%) and 655 (3.4%) samples of PCa and EC, respectively. Mutations clustered in the SPOP MATH domain with hotspots at residues F133L (21.4%), F102C (11%), F133V (10.4%), F102V (7%) in PCa, and residues M117V (21.8%), R121Q (10.6 %), E47K (9%), E50K (8.5%) in EC. Importantly, no overlap of hotspot residues was noted in PCa and EC. SPOP-mutated PCa had higher frequency of APC (21.4% vs 5.8%) and BCOR (1.95% vs 0.2%) lesions, but SPOP-mutated EC had higher frequency of U2AF1 (4.28% vs 1.0%), POLE (5.24% vs 2.0%) and CASP8 (4.17% vs 1.79%) lesions (all q <0.05) compared to non-mutant SPOP. Analysis of epigenetic markers showed lower BRD2 (245.4 vs 312 TPM) transcripts in SPOP-mutated PCa, but higher BRD2 (227.0 vs 200.6) (all q <0.05) in SPOP-mutated EC, compared to non-mutant SPOP, the former diverging from earlier published data in PCa. Analysis of inferred immune cell infiltrates showed that in SPOP-mutated PCa, M1-MΦ (36.8% vs 48.1%), CD8 T cell (20.2% vs 30.4%) and DC (53.3% vs 79.4%) are decreased and M2-MΦ (100% vs 99.9%) are increased; while in SPOP-mutated EC, M1-MΦ (87.2% vs 83.3 %), CD8 T cells (56.8% vs 49.7%) and DC (94.9% vs 93.3%) are increased and M2-MΦ (98.8% vs 98.9%) are decreased (all q <0.05). In PCa, higher AR expression was found in SPOP-mutated vs non-mutated tumors (98.5% vs 97.3%, p=0.09), with improved OS outcomes after hormone targeting agents leuprolide (2125 vs 1803 days; HR: 0.71, 95% CI (0.51-0.99), p=0.04) and bicalutamide (2538 vs 1854 days; HR: 0.57, 95% CI (0.41-0.79), p<0.001). In EC, ER and PR expression was significantly lower in SPOP-mutated vs non-mutated tumors 50.8% vs 65.4% and 35.4 vs 49.5% (all q <0.05), respectively, although no difference in OS was noted in the overall EC population. These data indicate that SPOP-mutations drive different molecular and immune microenvironments in PCa and EC, with apparently opposing roles in these malignancies, and provide a rationale for tailored therapeutic approaches in SPOP-mutated PCa and EC. Citation Format: Ludimila Cavalcante, Sachin K. Desmukh, Benedito A. Carneiro, Kartik Angara, Tyler E. Mattox, Sharon Wu, Joanne Xiu, Philip Walker, Matthew Oberley, Chadi Nabhan, Emmanuel S. Antonarakis. Opposing roles of SPOP mutations in human prostate and endometrial cancers [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2023; Part 1 (Regular and Invited Abstracts); 2023 Apr 14-19; Orlando, FL. Philadelphia (PA): AACR; Cancer Res 2023;83(7_Suppl):Abstract nr 937.

Formation of academic self-concept and intrinsic value within and across three domains: Extending the reciprocal internal/external frame of reference model.

The reciprocal internal/external frame of reference (RI/E) combines two models of academic self-concept formation, namely the reciprocal effects model (REM) and the internal/external frame of reference (I/E) model. The REM assumes reciprocal relations between achievement and academic self-concept. The I/E model assumes contrast effects between achievement and self-concept across math and verbal domains. The RI/E model can be extended to more school subjects than one math and one verbal domain, and to other motivational constructs. We examined an extended RI/E model considering achievement, academic self-concept, and intrinsic value related to math, German and English. The sample consisted of 1939 German secondary school students. The three measurement waves covered Grades 5 to 7. Cross-lagged panel models were used to analyse the data. Within domains, there were reciprocal relations between achievement and academic self-concept but only unidirectional relations between former achievements and later intrinsic values. Across domains, there were significant negative relations between former math achievement and later self-concepts and intrinsic values in German and English, and between former German and English achievements and later self-concept and intrinsic value in math. These findings imply contrast effects across math and verbal domains. The pattern of relations among constructs was found to be generalizable across gender and school tracks and stable across measurement waves. The study provides further support for the validity of the RI/E model and its extension to English as another school subject and intrinsic value as another motivational construct.

Molecular cloning and function of two tumor necrosis factor receptor-associated factors genes (TRAF2 and TRAF4) from Pinctada fucata martensii

Tumor necrosis factor receptor-associated factors (TRAFs) have been studied in a few mollusks and participate in various biological processes, like apoptosis, immune response, stress, and inflammatory response. However, TRAFs’ function and mechanism of pearl oysters (Pinctada fucata martensii) are still unclear. In this study, the novel PmTRAF2 and PmTRAF4 from P. f. martensii were cloned by rapid amplification of complementary DNA ends and their mRNA expression were analyzed by quantitative real-time PCR (qPCR). The interacting protein of PmTRAF2 was verified by the yeast two-hybrid assay. The result shows that full-length of PmTRAF2 and PmTRAF4 cDNA were 2055 bp and 2365 bp, respectively. The deduced PmTRAF2 and PmTRAF4 proteins contain TRAF-type zinc finger domain and MATH domain, while PmTRAF4 lacks a RING finger domain. Multiple sequence alignment revealed that PmTRAF2 and PmTRAF4 had high homology with the ortholog of other species. Phylogenic analysis indicated that PmTRAF4 clustered with the homolog protein of Mytilus edulis and Mytilus galloprovincialis, and PmTRAF2 has the closest genetic relationship to Crassostrea gigas TRAF2. The qPCR analysis revealed that PmTRAF2 and PmTRAF4 were expressed in all six tissues, and both of them were significantly expressed in hepatopancreas and gill (p < 0.01). Under lipopolysaccharide (LPS) stimulation, polyinosinic acid (PolyI:C) stimulation, and nucleus insertion surgery, the transcripts of PmTRAF2, PmTRAF3, PmTRAF4 and PmTRAF6 in hepatopancreas were markedly changed at corresponding time points. These results have indicated that these genes may play a role in P. f. martensii innate immunity. Yeast two-hybrid assays show that PmTRAF2 interacts with PmTRAF6 but not PmTRAF3, potentially affecting downstream immune signaling pathways. Our findings provide new perspectives for further investigation of TRAFs’ immune mechanisms in bivalves.

The impacts of math anxiety, science anxiety, and gender on arts versus sciences choices in Qatari secondary schools.

Previous studies showed small-to-moderate associations between students' performances in math and science and math anxiety and science anxiety, respectively. Accordingly, the high prevalence of these two forms of topic anxiety represent severe obstructions to the worldwide demand calling for improving the quality of math and science achievements and, subsequently, increasing career success in science, technology, engineering, and math (STEM) domains. Therefore, this study examined math anxiety and science anxiety among female and male students who were enrolled in Sciences vs Arts tracks in Grades 11 and 12 in a Middle Eastern Arabic-speaking country (Qatar), and investigated how gender, math anxiety and science anxiety could predict this enrollment. Results showed that students in the Arts track experienced higher levels of math anxiety and science anxiety than those in the Sciences track, regardless of the students' gender. However, a binary logistic regression analysis showed that science learning anxiety, but not evaluation science anxiety nor math learning or evaluation anxieties, significantly predicts students' enrollment in Arts and Sciences tracks. Therefore, STEM career success is associated with good knowledge of STEM domains and positive emotions towards math and science.