Massive Complexes Research Articles

Understanding the star formation efficiency in dense gas: Initial results from the CAFFEINE survey with ArTéMiS

Context. Despite recent progress, the question of what regulates the star formation efficiency (SFE) in galaxies remains one of the most debated problems in astrophysics. According to the dominant picture, star formation (SF) is regulated by turbulence and feedback, and the SFE is ~1–2% or less per local free-fall time on all scales from Galactic clouds to high-redshift galaxies. In an alternate scenario, the star formation rate (SFR) in galactic disks is linearly proportional to the mass of dense gas above some critical density threshold ~104 cm–3. Aims. We aim to discriminate between these two pictures thanks to high-resolution submillimeter and mid-infrared imaging observations, which trace both dense gas and young stellar objects (YSOs) for a comprehensive sample of 49 nearby massive SF complexes out to a distance of d ~ 3 kpc in the Galactic disk. Methods. We used data from CAFFEINE, a complete 350/450 µm survey with APEX/ArTéMiS of the densest portions of all southern molecular clouds at d ≲ 3 kpc, in combination with Herschel data to produce column density maps at a factor of ~4 higher resolution (8") than standard Herschel column density maps (36″). Our maps are free of any saturation effect around luminous high-mass pro-tostellar objects and resolve the structure of dense gas and the typical ~0.1 pc width of molecular filaments out to 3 kpc, which is the most important asset of the present study and is impossible to achieve with Herschel data alone. Coupled with SFR estimates derived from Spitzer mid-infrared observations of the YSO content of the same clouds, this allowed us to study the dependence of the SFE on density in the CAFFEINE clouds. We also combine our findings with existing SF efficiency measurements in nearby clouds to extend our analysis down to lower column densities. Results. Our results suggest that the SFE does not increase with density above the critical threshold and support a scenario in which the SFE in dense gas is approximately constant (independent of free-fall time). However, the SF efficiency measurements traced by Class I YSOs in nearby clouds are more inconclusive, since they are consistent with both the presence of a density threshold and a dependence on density above the threshold. Overall, we suggest that the SF efficiency in dense gas is primarily governed by the physics of filament fragmentation into protostellar cores.

Most nearby young star clusters formed in three massive complexes.

Efforts to unveil the structure of the local interstellar medium and its recent star-formation history have spanned the past 70 years (refs. 1-6). Recent studies using precise data from space astrometry missions have revealed nearby, newly formed star clusters with connected origins7-12. Nonetheless, mapping young clusters across the entire sky back to their natal regions has been hindered by a lack of clusters with precise radial-velocity data. Here we show that 155 out of 272 (57%) high-quality young clusters13,14 within 1 kiloparsec of the Sun arise from three distinct spatial volumes. This conclusion is based on the analysis of data from the third Gaia release15 and other large-scale spectroscopic surveys. At present, dispersed throughout the solar neighbourhood, their past positions more than 30 million yearsago reveal that these families of clusters each formed in one of three compact, massive star-forming complexes. One of these families includes all of the young clusters near the Sun-the Taurus and Scorpius-Centaurus star-forming complexes16,17. We estimate that more than 200 supernovae were produced from these families and argue that these clustered supernovae produced both the Local Bubble18 and the largest nearby supershell GSH 238+00+09 (ref. 19), both of which are clearly visible in modern three-dimensional dust maps20-22.

Structural determination and modeling of ciliary microtubules.

The axoneme, a microtubule-based array at the center of every cilium, has been the subject of structural investigations for decades, but only recent advances incryo-EM and cryo-ET have allowed a molecular-level interpretation of the entire complex to be achieved. The unique properties of the nine doublet microtubules and central pair of singlet microtubules that form the axoneme, including the highly decorated tubulin lattice and the docking of massive axonemal complexes, provide opportunities and challenges for sample preparation, 3D reconstruction and atomic modeling. Here, the approaches used for cryo-EM and cryo-ET of axonemes are reviewed, while highlighting the unique opportunities provided by the latest generation of AI-guided tools that are transforming structural biology.

Pavement freezing depth estimation using hybrid deep-learning models

Predicting pavement temperature by depth is crucial for road design, analysis, and maintenance. However, current methods predominantly utilize regression and/or open-form solutions focusing on highways. Additionally, most machine-learning models focus on asphalt layers and do not extend to deeper pavement layers. Therefore, this study provides deep-learning models using weather parameters to predict pavement temperature from surface to sublayers and estimate pavement freezing depth for developing massive apartment complexes. Temperature-by-depth data collected from thin pavements from three locations in South Korea were used. Comparative analyses of long short-term memory (LSTM), convolutional neural network-LSTM (CNN-LSTM), and convolutional LSTM were performed. Results showed that CNN-LSTM model performed better with coefficients of determination ( R2) of 0.965, 0.987, and 0.981. Additionally, the CNN-LSTM predicted freezing depth with 0.3%–13.1% error margins outperforming the LSTM, Aldrich’s, and Korean Ministry of Transport approaches. The proposed approach shows that deep-learning models better estimate the freezing depth of pavements than existing approaches.

Mitochondrial microproteins: critical regulators of protein import, energy production, stress response pathways, and programmed cell death.

Mitochondria rely upon the coordination of protein import, protein translation, and proper functioning of oxidative phosphorylation (OXPHOS) complexes I-V to sustain the activities of life for an organism. Each process is dependent upon the function of profoundly large protein complexes found in the mitochondria [translocase of the outer mitochondrial membrane (TOMM) complex, translocase of the inner mitochondrial membrane (TIMM) complex, OXPHOS complexes, mitoribosomes]. These massive protein complexes, in some instances more than one megadalton, are built up from numerous protein subunits of varying sizes, including many proteins that are ≤100-150 amino acids. However, these small proteins, termed microproteins, not only act as cogs in large molecular machines but also have important steps in inhibiting or promoting the intrinsic pathway of apoptosis, coordinate responses to cellular stress, and even act as hormones. This review focuses on microproteins that occupy the mitochondria and are critical for its function. Although the microprotein field is relatively new, researchers have long recognized the existence of these mitochondrial proteins as critical components of virtually all aspects of mitochondrial biology. Thus, recent studies estimating that hundreds of new microproteins of unknown function exist and are missing from current genome annotations suggests that the mitochondrial "microproteome" is a rich area for future biological investigation.

ABYSS. I. Targeting Strategy for the APOGEE and BOSS Young Star Survey in SDSS-V

The fifth iteration of the Sloan Digital Sky Survey is set to obtain optical and near-infrared spectra of ∼5 million stars of all ages and masses throughout the Milky Way. As a part of these efforts, APOGEE and BOSS Young Star Survey (ABYSS) will observe ∼105 stars with ages <30 Myr that have been selected using a set of homogeneous selection functions that make use of different tracers of youth. The ABYSS targeting strategy we describe in this paper is aimed to provide the largest spectroscopic census of young stars to date. It consists of eight different types of selection criteria that take the position on the H-R diagram, infrared excess, variability, as well as the position in phase space in consideration. The resulting catalog of ∼200,000 sources (of which a half are expected to be observed) provides representative coverage of the young Galaxy, including both nearby diffuse associations as well as more distant massive complexes, reaching toward the inner Galaxy and the Galactic center.

Structural studies put phage defense mystery on the RADAR.

Phage restriction by adenosine deaminase acting on RNA (RADAR) is a process by which bacteria may alter their own transcriptome to resist bacteriophage. In this issue of Cell, Duncan-Lowey and Tal etal. and Gao etal. both show RADAR proteins assemble into massive molecular complexes but present distinct views about how these assemblies obstruct phage.

Understanding Physiological Basis of Senescence in Ailanthus excelsa Leaves

Leaf senescence is a major developmental event, in which leaves turns yellowing and cells passes through a series of physiological and biochemical changes, including a large metabolic shift from anabolism to catabolism, resulting in nutrition redistribution. Since dynamic protein trafficking transforms massive macromolecular complexes into transportable products through which cellular structures such as chloroplasts, nucleus and cell membrane are disintegrated. The chlorophyll fluorescence measurement can detect the physiological changes in leaves during senescence. Therefore, the OJIP-transient, leaf model and membrane model were studied to understand the photosynthetic process during senescence. The depression OJIP-transient curve and Fm shows reduction in PIcs. The increase in antenna size of LHCs ABS/RC and TR/RC increased but the phenomenological fluxes reduced, which illustrates the inhibition of electron transport chain in S5 (last stage of senescence). Although the absorption increased, but electron transport ceased so that DI/CS and DI/RC enhanced which directly influence ΦPo and the leaf ultimately die.

The Efficacy and Safety of Oral Nutrient Solution Supplementation in Patients with Esophageal Cancer under Nutritional, Psychological, Physical, and Prognostic Survival Differences.

Inflammasomes are massive intracellular multiprotein signaling complexes produced in the cytosolic compartment. Tumor etiology involves dysregulation of NLRP3 inflammasome activation. Current studies are being conducted to ascertain the effectiveness and safety of oral nutrient solution supplementation in patients with esophageal cancer based on the current understanding of the role of the NLRP3 inflammasome in possible cancer promotion and therapy. This study examines how oral nutrient solution supplement therapy affects patients receiving radiotherapy and chemotherapy for esophageal cancer in terms of nutrition, physical composition, quality of life, psychology, prognosis, and survival. A total of 120 patients with esophageal cancer admitted to our hospital from March 2020 to January 2021 were selected for the current study. The selected 120 patients are divided into nutritional supplement and traditional groups according to the random number table method, with 60 cases in each group. The nutritional supplement group received an oral nutritional solution, whereas the conventional group received the ordinary dietary intervention. Since radiotherapy can potentially damage healthy tissue with short- and long-term negative effects. Inflammatory reactions are a common component of adverse effects of early and late ionizing radiation. Oral nutrient solution supplement therapy for patients with esophageal cancer after radiotherapy and chemotherapy can significantly improve the levels of blood nutrients, later physical components, and quality of life of patients.

Filamentary structures of ionized gas in Cygnus X

Context.Ionized gas probes the influence of massive stars on their environment. The Cygnus X region (d~ 1.5 kpc) is one of the most massive star-forming complexes in our Galaxy, within which the Cyg OB2 association (age of 3–5 Myr and stellar mass 2 × 104M⊙) has a dominant influence.Aims.We observe the Cygnus X region at 148 MHz using the Low Frequency Array (LOFAR) and take short-spacing information into account during image deconvolution into account. Together with data from the Canadian Galactic Plane Survey, we investigate the morphology, distribution, and physical conditions of low-density ionized gas in a 4° × 4° (~100 pc × 100 pc) region at a resolution of 2′ (0.9 pc).Methods.The Galactic radio emission in the region analyzed is almost entirely thermal (free-free) at 148 MHz, with emission measures (EM) of 103&lt; EM [pc cm−6] &lt; 106. As filamentary structure is a prominent feature of the emission, we use DisPerSE and Fil ChaP to identify filamentary ridges and characterize their radial (EM) profiles.Results.The distribution of radial profiles has a characteristic width of 4.3 pc and a power-law distribution (β= −1.8 ± 0.1) in peak EM down to our completeness limit of 4200 pc cm−6. The electron densities of the filamentary structure range between 10 ≲ne[cm−3] ≲ 400 with a median value of 35 cm−3, remarkably similar to [N II] surveys of ionized gas.Conclusions.Cyg OB2 may ionize at most two-thirds of the total ionized gas and the ionized gas in filaments. More than half of the filamentary structures are likely photoevaporating surfaces flowing into a surrounding diffuse (~5 cm−3) medium. However, this is likely not the case for all ionized gas ridges. A characteristic width in the distribution of ionized gas indicates that the stellar winds of Cyg OB2 create a fraction of the ionized filaments through swept-up ionized gas or dissipated turbulence.

Volcanic Massive Sulfide Complexes of the Magnitogorsk Paleoisland Arc Megazone in the Southern Urals: Models of Ore-Magmatic System and Geodynamic Reconstructions

Volcanic Massive Sulfide Complexes of the Magnitogorsk Paleoisland Arc Megazone in the Southern Urals: Models of Ore-Magmatic System and Geodynamic Reconstructions

Unexpected benzene oxidation in collisions with superoxide anions

Superoxide anions colliding with benzene molecules at impact energies from 200 to 900 eV are reported for the first time to form massive complexes. With the aid of quantum chemistry calculations, we propose a mechanism in which a sudden double ionization of benzene and the subsequent electrostatic attraction between the dication and the anion form a stable covalently bonded C6H6O2+ molecule, that evolves towards the formation of benzene-diol conformers. These findings lend support to a model presenting a new high energy anion-driven chemistry as an alternative way to form complex molecules.

Cytoplasmic factories for axonemal dynein assembly.

Axonemal dyneins power the beating of motile cilia and flagella. These massive multimeric motor complexes are assembled in the cytoplasm, and subsequently trafficked to cilia and incorporated into the axonemal superstructure. Numerous cytoplasmic factors are required for the dynein assembly process, and, in mammals, defects lead to primary ciliary dyskinesia, which results in infertility, bronchial problems and failure to set up the left-right body axis correctly. Liquid-liquid phase separation (LLPS) has been proposed to underlie the formation of numerous membrane-less intracellular assemblies or condensates. In multiciliated cells, cytoplasmic assembly of axonemal dyneins also occurs in condensates that exhibit liquid-like properties, including fusion, fission and rapid exchange of components both within condensates and with bulk cytoplasm. However, a recent extensive meta-analysis suggests that the general methods used to define LLPS systems in vivo may not readily distinguish LLPS from other mechanisms. Here, I consider the time and length scales of axonemal dynein heavy chain synthesis, and the possibility that during translation of dynein heavy chain mRNAs, polysomes are crosslinked via partially assembled proteins. I propose that axonemal dynein factory formation in the cytoplasm may be a direct consequence of the sheer scale and complexity of the assembly process itself.

VERITAS Observations of the Galactic Center Region at Multi-TeV Gamma-Ray Energies

Abstract The Galactic Center (GC) region hosts a variety of powerful astronomical sources and rare astrophysical processes that emit a large flux of nonthermal radiation. The inner 375 pc × 600 pc region, called the Central Molecular Zone, is home to the supermassive black hole Sagittarius A*, massive cloud complexes, and particle accelerators such as supernova remnants (SNRs). We present the results of our improved analysis of the very-high-energy gamma-ray emission above 2 TeV from the GC using 125 hr of data taken with the Very Energetic Radiation Imaging Telescope Array System imaging-atmospheric Cerenkov telescope between 2010 and 2018. The central source VER J1745–290, consistent with the position of Sagittarius A*, is detected at a significance of 38 standard deviations above the background level (38σ), and we report its spectrum and light curve. Its differential spectrum is consistent with a power law with exponential cutoff, with a spectral index of , a flux normalization at 5.3 TeV of TeV−1 cm−2 s−1, and cutoff energy of TeV. We also present results on the diffuse emission near the GC, obtained by combining data from multiple regions along the GC ridge, which yield a cumulative significance of 9.5σ. The diffuse GC ridge spectrum is best fit by a power law with a hard index of 2.19 ± 0.20, showing no evidence of a cutoff up to 40 TeV. This strengthens the evidence for a potential accelerator of PeV cosmic rays being present in the GC. We also provide spectra of the other sources in our field of view with significant detections, composite SNR G0.9+0.1, and HESS J1746–285.

Phylogenomics, Origin, and Diversification of Anthozoans (Phylum Cnidaria).

Anthozoan cnidarians (corals and sea anemones) include some of the world's most important foundation species, capable of building massive reef complexes that support entire ecosystems. Although previous molecular phylogenetic analyses have revealed widespread homoplasy of the morphological characters traditionally used to define orders and families of anthozoans, analyses using mitochondrial genes or rDNA have failed to resolve many key nodes in the phylogeny. With a fully resolved, time-calibrated phylogeny for 234 species constructed from hundreds of ultraconserved elements and exon loci, we explore the evolutionary origins of the major clades of Anthozoa and some of their salient morphological features. The phylogeny supports reciprocally monophyletic Hexacorallia and Octocorallia, with Ceriantharia as the earliest diverging hexacorals; two reciprocally monophyletic clades of Octocorallia; and monophyly of all hexacoral orders with the exception of the enigmatic sea anemone Relicanthus daphneae. Divergence dating analyses place Anthozoa in the Cryogenian to Tonian periods (648-894 Ma), older than has been suggested by previous studies. Ancestral state reconstructions indicate that the ancestral anthozoan was a solitary polyp that had bilateral symmetry and lacked a skeleton. Colonial growth forms and the ability to precipitate calcium carbonate evolved in the Ediacaran (578 Ma) and Cambrian (503 Ma) respectively; these hallmarks of reef-building species have subsequently arisen multiple times independently in different orders. Anthozoans formed associations with photosymbionts by the Devonian (383 Ma), and photosymbioses have been gained and lost repeatedly in all orders. Together, these results have profound implications for the interpretation of the Precambrian environment and the early evolution of metazoans.[Bilateral symmetry; coloniality; coral; early metazoans; exon capture; Hexacorallia; Octocorallia photosymbiosis; sea anemone; ultraconserved elements.].

Como esclavos y no vasallos: reclamaciones interétnicas y no - libertad en el Perú colonial

Across much of the Spanish empire in the Americas, indigenous and African-descent peoples lived in close contact. The entangled nature of labor, both in urban centers and on massive complexes, gave them the opportunity to measure themselves against one another. Law that developed across the sixteenth and seventeenth centuries suggested an eventual clarity about their separate conditions, but experience revealed the muddiness of both definitions and enforcement. Indigenous and Black subjects used those colonial discourses about freedom and hierarchy to understand their own positions and to argue for comparable protections and privileges. Rather than consider indigenous and Black lives separately, the essay argues for a more integrative approach to reading legal documents produced by and about them.

Neospora caninum: Differential Proteome of Multinucleated Complexes Induced by the Bumped Kinase Inhibitor BKI-1294.

Background: the apicomplexan parasite Neospora caninum causes important reproductive problems in farm animals, most notably in cattle. After infection via oocysts or tissue cysts, rapidly dividing tachyzoites infect various tissues and organs, and in immunocompetent hosts, they differentiate into slowly dividing bradyzoites, which form tissue cysts and constitute a resting stage persisting within infected tissues. Bumped kinase inhibitors (BKIs) of calcium dependent protein kinase 1 are promising drug candidates for the treatment of Neospora infections. BKI-1294 exposure of cell cultures infected with N. caninum tachyzoites results in the formation of massive multinucleated complexes (MNCs) containing numerous newly formed zoites, which remain viable for extended periods of time under drug pressure in vitro. MNC and tachyzoites exhibit considerable antigenic and structural differences. Methods: Using shotgun mass spectrometry, we compared the proteomes of tachyzoites to BKI-1294 induced MNCs, and analyzed the mRNA expression levels of selected genes in both stages. Results: More than half of the identified proteins are downregulated in MNCs as compared to tachyzoites. Only 12 proteins are upregulated, the majority of them containing SAG1 related sequence (SRS) domains, and some also known to be expressed in bradyzoites Conclusions: MNCs exhibit a proteome different from tachyzoites, share some bradyzoite-like features, but may constitute a third stage, which remains viable and ensures survival under adverse conditions such as drug pressure. We propose the term “baryzoites” for this stage (from Greek βαρυσ = massive, bulky, heavy, inert).

In‐Vivo Knockdown of Cortactin Disrupts Basal Tubulobulbar Complexes and Results in the Presence of Redundant Junction Related Ectoplasmic Specializations

In the mammalian testis, spermatogenic cells begin development in the germ cell niche or basal compartment of the seminiferous epithelium. They then translocate through massive junction complexes between neighboring Sertoli cells to enter the adluminal compartment and further differentiate. Removal of the junctions above and formation of new junctions below is necessary for the translocation of cells from one compartment to the other. Tubulobulbar complexes’ (TBCs) are Sertoli cell specific structures that are proposed to internalize intercellular junctions in the seminiferous epithelium. TBCs are actin cuffed endocytic structures that share many features in common with clathrin mediated endocytosis (CME). Both TBCs and CME vesicles begin with the association of a clathrin coated pit on the plasma membrane and the development of dendritic actin ‘cuffs’ on their neck regions. Rather than vesiculating, the necks of TBCs continue to lengthen into microns long structures, eventually developing an actin free ‘bulb’ region, which develops an ORP9 positive membrane contact site with an associated leaflet of endoplasmic reticulum. One of the proteins known to be highly specific for the dendritic actin around TBCs is the actin related protein, cortactin. It previously has been shown that knockdown of cortactin causes shortening and disorganization of those TBCs at the apex of the epithelium that are associated with late spermatids, and this is correlated with a delay in sperm release. However, it has not been shown if disruption of cortactin expression has similar effects on TBCs associated with the basal junction complexes and whether or not this has an effect on junction removal. Here we use electron microscopy (EM) and stimulated emission depletion (STED) super resolution microscopy to evaluate the phenotype of rat testes injected 5 times over a 9‐day period with siRNA targeting cortactin, and use western blots to confirm the knockdown. In vivo knockdown of cortactin in rat testes disrupts basal TBCs when sections labeled for ORP9 and cortactin/actin are evaluated by STED, and leads to the presence of redundant junction related material (ectoplasmic specializations) when evaluated by EM. These data are consistent with the conclusion that TBCs internalize basal junctions and are likely part of the mechanism of spermatocyte translocation from basal to adluminal compartments of the epithelium.Support or Funding InformationSupported by a Natural Sciences and Engineering Research Council of Canada (NSERC) Discovery Grant to A Wayne Vogl (RGPIN‐2018‐03727).

A matter of Matter. The Unmaking of Microscopic Bonds in Transnational Space

Over the last decades, new architectures and infrastructures for containing and artificially reproducing the conditions at the very beginning of the Universe have surfaced across the world: a synthetic replica of the early Universe on Earth. These sophisticated technospaces are sealed chambers that recreate specific and inexistent physical conditions on Earth to reveal the origin of matter. They spread throughout a subterranean global chamber system: an invisible underground network. New supranational laboratories like CERN transformed physics into a force within politics, creating new spaces of negotiations and agreement. Subatomic particles are questioning the Standard Model of Particle Physics while scientific infrastructures simultaneously challenge previous models for political and social structures. The tiny monastic laboratory of the seventeenth century has been replaced by a global technoscientific infrastructure spread across the world. The elementary particle physics infrastructures involve massive urban complexes, buildings, and chambers, designed to host the invisible. These hybrid urban environments are comprised of scientists, particles, liquids, data, politics and technologies working together for the production of knowledge, and to translate a world not perceptible to humans. The transnational network of Particle Physics underground laboratories is constructing a new scientific architecture around the world: a sensing architecture, which amplifies new political and material interactions. Maybe, we can say that every new scientific experimental chamber is the architect of a new epoch of hybrid systems of transnational and transhuman collaborations. This text is part of a larger project conducted through a practice-led PhD in Arts and Sciences at the BxNU Institute (Baltic Centre for Contemporary Art and Northumbria University).

Nature Empowered: Hydraulic Models and the Engineering of Niagara Falls.

Under a 1950 U.S.-Canada treaty the majority of the Niagara River is diverted to massive hydroelectric complexes. To hide the aesthetic impact of turning Niagara Falls into an energy waterscape, and retain tourism, the waterfall was physically reshaped and shrunk, and remedial works were built to control water flows and hydropower diversions. To achieve this, hydraulic engineers from the U.S. Army Corps of Engineers and Ontario Hydro relied on hydraulic models of Niagara Falls. Using an envirotech approach, I show how these physical hydraulic models were an integral part of the disguised design approach to remaking Niagara Falls, and I use this Niagara case study to critically explore the evolution of hydraulic modeling in early Cold War North America. The river and waterfall were transformed into a hybrid envirotechnical system, a submerged infrastructure that relied not only on steel and concrete, but also water, ice, and weeds.