Mass Redistribution Research Articles

Assessments of GPS satellite orbiting period effects on diurnal and semi-diurnal luni-solar declinations utilizing Galileo satellites

Global Navigation Satellite Systems (GNSS) can observe a variety of surface deformations on Earth, including periodic oscillations at different frequencies. An example of such phenomena is ocean tide loadings (OTL), which result from the redistribution of water mass. The Global Positioning System (GPS) exhibits orbital geometry that causes its revisit and orbital periods to coincide with the diurnal and semi-diurnal luni-solar declination constituents, known as K1 and K2, respectively. Consequently, the system faces challenges in accurately estimating these periodic oscillations due to its orbital artifacts. This study aims to quantify the extent to which GPS orbital artifacts introduce periodic signals into the K1 and K2 constituents by utilizing the Galileo system and determining the most suitable positioning approach. A dataset from the International GNSS Service (IGS), spanning 40 days in 2024 and covering six stations, was analyzed. Coordinates were estimated using both kinematic positioning every 5 minutes and a 6-hour static precise point positioning (PPP) mode with a 3-hour shift. The power spectra for the east, north, and up components indicated that, on average, the GPS system contributes 52.8% to the K1 constituents and 66.3% to the K2 constituents. Despite expectations that the diurnal K1 and semi-diurnal K2 tidal constituents would be more prominent in the power spectra of the GPS comparing to that of natural signature or of other navigation system (Galileo for this study), the diurnal K1 tidal constituent appeared weak in the kinematic mode power spectra for the GPS system. These findings validate that the overlapped-static PPP mode is a more appropriate approach for estimating these periodic deformations.

Comparison of automated and manual intracellular particle tracking using quantitative phase imaging

Transport within cells is commonly studied using particle tracking methods. However, these typically require either labeling or identification of specific organelles that can be identified and tracked from label-free imaging modalities, limiting application of this approach. Quantitative phase imaging (QPI) provides dynamic data on the redistribution of mass within live cells, potentially enabling broader application of particle tracking methods. In previous work, we developed quantitative phase velocimetry (QPV) to automatically track the motion of subcellular control volumes from QPI data. However, the relationship of QPV to traditional particle tracking methods has not been established. Here, we directly compare QPV to manual particle tracking across multiple drug treatment conditions. We find that QPV effective diffusivity is correlated with diffusivity measured from manual particle tracking. The differences between QPV and manual tracking are explained by the difference in effective size of particles tracked by QPV. Overall, these data indicate that automated tracking of the motion of cellular mass from QPI data can effectively be used to characterize effective diffusivity within living cells.

About the improvement in Mars Polar Motion determination from radio tracking of two landers

The polar motion of Mars is defined as the movement of the rotation axis with respect to a body-fixed frame tied to the crust of the planet. It is composed of forced motion at annual and sub-annual frequencies caused by the seasonal mass redistribution, formation of the polar ice caps and angular momentum variations of the atmosphere, and of the free mode called the Chandler wobble.Radio-tracking data from landers offers the most suitable means to measure the rotation of Mars, including its polar motion. The latter, however, has not yet been achieved using lander data alone. In this study, we assess the uncertainties associated with Mars polar motion estimation using Direct-To-Earth Doppler, range and Same-Beam Interferometry (SBI) observables between multiple landers on the surface of Mars. We evaluate the improvement enabled by combining data from multiple landers with respect to one-lander scenarios, and identify the optimal mission architectures for polar motion estimation by considering the influence of respective mission parameters on the estimation uncertainty. In particular, we consider the effects of absolute and relative locations of the landers and of mission scheduling. We re-evaluate the possibility of estimating the polar motion using data from landers in proximity to the equator, and apply our considerations to simulated data consistent in number and accuracy with that collected by past Martian missions. We notice and explain a strong longitude dependence of the formal errors when the polar motion parameters are estimated concurrently with the seasonal spin variation parameters, making it impossible to properly determine all components of polar motion with a single lander regardless of its location. However, the use of two or more landers in optimal locations with respect to each other eliminates those limitations. We evaluate the influence of latitudinal and longitudinal separation on polar motion determination in such cases. In particular, we are able to determine polar motion well even in cases where the longitudes of the two landers make determination from each single lander impossible.

Giant impact on early Ganymede and its subsequent reorientation

Ganymede has an ancient impact structure called a furrow system. The furrow system is the largest impact structure in the outer solar system, and the impact should have significantly affected Ganymede’s early history; however, its effects are poorly understood. No attention has been given to the center of the furrow system coinciding with Ganymede's tidal axis, indicating that mass redistribution induced by the furrow-forming impact caused a reorientation (true polar wander) of Ganymede. We propose that the impact ejecta created a mass anomaly that reoriented the impact site toward the tidal axis. We found that an impactor with a radius of 150 km and an incidence angle between 60° and 90° most accurately reproduces the current location of the furrow system. We predict that future explorations would reveal remnant topographic profiles or gravity anomalies associated with the furrow-forming impact and reorientation. Additionally, various possible explanations for the reorientation of Ganymede, such as an impactor-origin mascon beneath the basin or a thickness variation in the lithosphere, should be studied.

Revisiting the Excitation of Free Core Nutation

AbstractEarth possesses a Poincaré mode called Free Core Nutation (FCN) due to the misalignment of the rotation axes of the mantle and fluid outer core. FCN is the primary signal in the observations of Celestial Pole Offsets (CPO) and maintained by geophysical mechanisms that are yet to be understood. Earlier studies suggested an origin in Atmospheric Angular Momentum (AAM)—and to a lesser degree Oceanic Angular Momentum (OAM)—but discrepancies between these geophysical excitations and the geodetic (CPO‐based) excitation were too large to reach definite conclusions. Here we use newly calculated, 3‐hourly AAM and OAM series for the 1994–2022 period, in conjunction with the latest CPO series from the International Earth Rotation and Reference Systems Service (IERS 20 C04 series), to demonstrate a markedly lower power ratio (4.6) of geophysical over geodetic excitation at the FCN frequency compared to previous works (ratio 10). Among all excitation sources, the AAM pressure term exhibits the highest coherence (0.56) and correlation (0.48) with the geodetic excitation, whereas the coherence with OAM is smaller by a factor of 3. Similar analyses using existing angular momentum series give comparable, albeit smaller coherence and correlation results. We attribute the relevant AAM pressure term signal to Northern Hemispheric landmasses and further show consistent temporal variations in the amplitude of geophysical and geodetic excitations around the FCN band. Our results thus corroborate evidence for large‐scale atmospheric mass redistribution to be the main cause of continuous FCN excitation.

Abstract P139: Exploring the cardiometabolic effects of lipid 12-hydroxyeicosapentaenoic acid through thromboxane receptor partial agonism

Introduction: It is well known that lipid mediators secreted by brown adipose tissue (BAT) during cold exposure or beta-adrenergic stimulation can exert beneficial effects on glucose and lipoprotein homeostasis. Recently, Leiria et al. demonstrated that one of these mediators, 12-hydroxyeicosapentaenoic acid (12-HEPE), secreted by BAT, enhances glucose tolerance and insulin sensitivity through the stimulation of a G protein-coupled receptor (GPCR). However, the receptor that 12(S)-HEPE binds to exert its functions was unknown. Therefore, this study aimed to identify the GPCR that 12-HEPE binds to and to investigate whether 12-HEPE affects vascular reactivity. Methods: Screening of 70 non-olfactory GPCR candidates was conducted in HEK293 cells, either stimulated or not with 12-HEPE (5µM). Receptor activation was measured using the dynamic mass redistribution (DRM) assay. After identifying the potential receptor, we validated the result by monitoring intracellular Ca2+ levels through a dose-response curve for 12-HEPE. To study the biological effects resulting from this ligand-receptor interaction, thoracic aortic artery rings were isolated from 12-week-old C57BL6 male mice, and their contractile responses were monitored using a myograph in response to cumulative doses ranging from 100pM to 10µM of 12-HEPE. Results: Through DRM screening, we found that the thromboxane receptor (TP) exhibited the strongest interaction with 12(S)-HEPE (5µM). To validate the screening, we incubated HEK293 cells with 12-HEPE or U46619 and quantified intracellular calcium mobilization. Our data demonstrate that 12-HEPE is a partial TP agonist, with an EC50 of 314nM compared to 7.2nM for U46619. Furthermore, incubation of the thoracic aorta with 12-HEPE produced dose-dependent increases in arterial constriction (pEC50: 6.129 ± 0.174; Emax: 95.39 ± 15.72), and this effect was completely prevented by the thromboxane antagonist, TPA (1µM). Conclusions: Our results suggest that 12-HEPE is an endogenous partial agonist of the TP receptor, and such interaction is necessary for the biological effects of these lipids. Importantly, the vasoconstrictor effect mediated by 12-HEPE may be an important physiological adaptation to prevent body heat loss upon cold exposure, thus allowing for adequate temperature maintenance.

In vitro pharmacological characterization of growth hormone secretagogue receptor ligands using the dynamic mass redistribution and calcium mobilization assays

Ghrelin modulates several biological functions via selective activation of the growth hormone secretagogue receptor (GHSR). GHSR agonists may be useful for the treatment of anorexia and cachexia, while antagonists and inverse agonists may represent new drugs for the treatment of metabolic and substance use disorders. Thus, the identification and pharmacodynamic characterization of new GHSR ligands is of high interest. In the present work the label-free dynamic mass redistribution (DMR) assay has been used to evaluate the pharmacological activity of a panel of GHSR ligands. This includes the endogenous peptides ghrelin, desacyl-ghrelin and LEAP2(1–14). Among synthetic compounds, the agonists anamorelin and HM01, the antagonists HM04 and YIL-781, and the inverse agonist PF-05190457 have been tested, together with HM03, R011, and H1498 from patent literature. The DMR results have been compared to those obtained in parallel experiments with the calcium mobilization assay. Ghrelin, anamorelin, HM01, and HM03 behaved as potent full GHSR agonists. YIL-781 behaved as a partial GHSR agonist and R011 as antagonist in both the assays. LEAP2(1–14) resulted a GHSR inverse agonist in DMR but not in calcium mobilization assay. PF-05190457, HM04, and H1498 behaved as GHSR inverse agonists in DMR experiments, while they acted as antagonists in calcium mobilization studies. In conclusion, this study provided a systematic pharmacodynamic characterization of several GHSR ligands in two different pharmacological assays. It demonstrated that the DMR assay can be successfully used particularly to discriminate between antagonists and inverse agonists. This study may be useful for the selection of the most appropriate compounds to be used in future studies.

Allosterism in the adenosine A2A and cannabinoid CB2 heteromer.

Allosterism is a regulatory mechanism for GPCRs that can be attained by ligand-binding or protein-protein interactions with another GPCR. We have studied the influence of the dimer interface on the allosteric properties of the A2A receptor and CB2 receptor heteromer. We have evaluated cAMP production, phosphorylation of signal-regulated kinases (pERK1/2), label-free dynamic mass redistribution, β-arrestin 2 recruitment and bimolecular fluorescence complementation assays in the absence and presence of synthetic peptides that disrupt the formation of the heteromer. Molecular dynamic simulations provided converging evidence that the heteromeric interface influences the allosteric properties of the A2AR-CB2R heteromer. Apo A2AR blocks agonist-induced signalling of CB2R. The disruptive peptides, with the amino acid sequence of transmembrane (TM) 6 of A2AR or CB2R, facilitate CB2R activation, suggesting that A2AR allosterically prevents the outward movement of TM 6 of CB2R for G protein binding. Significantly, binding of the selective antagonist SCH 58261 to A2AR also facilitated agonist-induced activation of CB2R. It is proposed that the A2AR-CB2R heteromer contains distinct dimerization interfaces that govern its functional properties. The molecular interface between protomers of the A2AR-CB2R heteromer interconverted from TM 6 for apo or agonist-bound A2AR, blocking CB2R activation, to mainly the TM 1/7 interface for antagonist-bound A2AR, facilitating the independent opening of intracellular cavities for G protein binding. These novel results shed light on a different type of allosteric mechanism and extend the repertoire of GPCR heteromer signalling.

Variational approaches to the elasticity of deformable strings with and without mass redistribution

AbstractThe catenary is a curve that has played a significant role in the history of mathematics, finding applications in various disciplines such as mechanics, technology, architecture, the arts, and biology. In this paper, we introduce some generalizations by applying the variational method to deformable strings. We explore two specific cases: (i) in the first case, we investigate the nonlinear behavior of an elastic string with variable length, dependent on the applied boundary conditions; specifically, this analysis serves to introduce the variational method and demonstrate the process of finding analytical solutions; (ii) in the second case, we examine a deformable string with a constant length; however, we introduce mass redistribution within the string through nonlinear elastic interactions. In the first scenario, the deformation state of the string always describes elongation, as compression states prove to be unstable for fully flexible strings. In contrast, in the second scenario, the finite length constraint induces compressive states in specific configurations and regions of the string. However, it is worth noting that the solution to this problem exists only for values of the elastic constant that are not too low, a phenomenon that is studied in detail. We conduct here both analytical and graphical analyses of various geometries, comparing the elastic behavior of the two aforementioned types of strings. Understanding the elastic behavior of deformable strings, especially the second type involving mass redistribution, is crucial for enhancing comprehension in the study of biological filaments or fibers and soft matter. For instance, these investigations can contribute to understanding the mechanisms employed by cells to sense gravity or other mechanical conditions.

Contributions of core, mantle and climatological processes to Earth’s polar motion

Earth’s spin axis slowly moves relative to the crust over time. A 120-year-long record of this polar motion from astronomical and more modern geodetic measurements displays interannual and multidecadal fluctuations of 20 to 40 milliarcseconds superimposed on a secular trend of about 3 milliarcseconds per year. Earth’s polar motion is thought to be driven by various surface and interior processes, but how these processes operate and interact to produce the observed signal remains enigmatic. Here we show that predictions made by an ensemble of physics-informed neural networks trained on measurements to capture geophysical processes can explain the main features of the observed polar motion. We find that glacial isostatic adjustment and mantle convection primarily account for the secular trend. Mass redistribution on the Earth’s surface—for example, ice melting and global changes in water storage—yields a relatively weak trend but explains about 90% of the interannual and multidecadal variations. We also find that core processes contribute to both the secular trend and fluctuations in polar motion, either due to variations in torque at the core–mantle boundary or dynamical feedback of the core in response to surface mass changes. Our findings provide constraints on core–mantle interactions for which observations are rare and global ice mass balance over the past century and suggest feedback operating between climate-related surface processes and core dynamics.

Multifractal characterization of fried foods pore microstructure

This study aimed to unravel the multifractal properties of internal microstructure in relation to textural evolution of meat-analog (MA) based coated fried foods. Wheat and rice flour-based batters were used to coat MA model that was deep fried at 180 °C for 2, 4 and 6 min in canola oil. Internal pore microstructures were investigated by X-ray microtomography based multifractal analysis (MFA) and texture were assessed by mechanical texture analyzer. Results showed that batter coatings greatly influenced the formation of internal microstructure during frying as well as post-frying mass redistribution and textural evolution. The frying time impacted the evolution of micropores, and porosity showed a positive correlation with the fat content of MA-based fried products. Porous, low-moisture and high-fat containing coated fried MA-based product demonstrated higher textural stability. Internal microstructure (pore distribution) of fried MAs were heterogenous and possessed multifractal scaling behavior, as depicted by MFA outcomes (Singularity & Rényi spectra). Strong correlation observed between moisture-fat, texture, porosity and selected multifractal parameters (Δα, Δfα, R-L, D1, D2, ΔD).

Determination of the time-variable geopotential by means of orbiting clocks

Monitoring the time-variable geopotential identifies the mass redistribution across the Earth and reveals, e.g., climate change and availability of water resources. The features of interest are characterized by spatial and temporal scales accessible only through space missions. Among the most important gravity missions are GRACE (2002–2017), its successor GRACE-FO (since 2018), and GOCE (2009–2013), which all sense the Earth’s gravity field via the geopotential derivatives. We investigate the geopotential estimation through frequency comparisons between orbiting clocks by means of the Doppler-canceling technique, describing the clocks’ behavior in the Earth’s gravitational field via Einstein’s general relativity. The novelty of this approach lies in measuring gravity by sensing the geopotential itself. The proof of principle for the measurement is achieved through an innovative mission scenario: for the first time, the observations are collected by a probing clock in LEO. We show gravity solutions obtained by simulating an estimation problem via our proposed architecture. The results suggest that we can conceivably retrieve the geopotential coefficients with accuracy comparable to the GRACE measurement concept by employing clocks with stabilities of order 10-18\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${10}^{-18}$$\\end{document}. Presently, terrestrial clocks can routinely attain fractional frequency stabilities of 10-18\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${10}^{-18}$$\\end{document}, whereas spaceborne clocks are still at the 10-15\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${10}^{-15}$$\\end{document} level. While our findings are promising, further analysis is needed to obtain more realistic indications on the feasibility of an actual mission, whose realization will be possible when clock technology reaches the required performance. The goal is for the technique investigated in this study to become a future staple for gravity field estimation.

The 3D stress field of Nordland, northern Norway – insights from numerical modelling

Abstract The Nordland area in northern Norway is the seismically most active area on mainland Fennoscandia. It exhibits patterns of coastal extension, which contrasts with the first-order regional stress pattern that reflects compression aligned with the North Atlantic ridge push. The regional stress field has been considered to emanate from the interaction of ridge push and glacial isostatic adjustment; while the local stress pattern can be additionally influenced by gravitational, topographic stresses, as well as the flexural effects of erosion and sediment deposition. We employ finite element numerical models at a crustal scale to study the 3D stress field, using existing geometric constraints from previous geophysical studies. Internal body forces, induced by variations in density, topography or Moho depth, already yield significant deviatoric stresses. In the models tested, these can strongly influence the near-surface stress regime, in particular for the continental margin setting we are investigating. In addition, redistribution of rock mass, which occurred mainly under Pleistocene glaciation, can modify the stress field significantly on a semi-regional scale. We consider this process to be the main driver for the coastal extension, in particular in areas where erosion has been high.

The Scatter Matters: Circumgalactic Metal Content in the Context of the M–σ Relation

The interaction between supermassive black hole (SMBH) feedback and the circumgalactic medium (CGM) continues to be an open question in galaxy evolution. In our study, we use smoothed particle hydrodynamics simulations to explore the impact of SMBH feedback on galactic metal retention and the motion of metals and gas into and through the CGM of L* galaxies. We examine 140 galaxies from the 25 Mpc cosmological volume Romulus25, with stellar masses between log(M */M ⊙) = 9.5–11.5. We measure the fraction of metals remaining in the interstellar medium (ISM) and CGM of each galaxy and calculate the expected mass of each SMBH based on the M BH–σ relation (Kormendy & Ho 2013). The deviation of each SMBH from its expected mass, ΔM BH, is compared to the potential of its host via σ. We find that SMBHs with accreted mass above M BH–σ are more effective at removing metals from the ISM than undermassive SMBHs in star-forming galaxies. Overall, overmassive SMBHs suppress the total star formation of their host galaxies and more effectively move metals from the ISM into the CGM. However, we see little to no evacuation of gas from the CGM out of their halos, in contrast with other simulations. Finally, we predict that C iv column densities in the CGM of L* galaxies are unlikely to depend on host galaxy SMBH mass. Our results show that the scatter in the low-mass end of the M BH–σ relation may indicate how effective an SMBH is in the local redistribution of mass in its host galaxy.

VARIABILITY OF EQUIVALENT WATER HEIGHT (EWH) IN INDONESIA DURING 14 YEARS OF GRACE GRAVITY SATELLITE MISSION

GRACE Satellite (Gravity Recovery and Climate Experiment) is a gravity monitoring satellite, that is very sensitive to mass changes, especially the signals that produce by redistribution of water masses. Data from this satellite can be used to observe water distribution in the form of spherical harmonic coefficients. Water mass variations are presented as Equivalent Water Height (EWH). The results of GRACE processing showed that the largest EWH was 27.298 cm in January 2015 and the smallest EWH was 29.816 cm in June 2004 in Sumatera Island. The positive trend occurred in Sumatra island and the negative trend occurred in eastern Indonesia. Generally, the trend of Indonesian rainfall throughout 2002 to 2016 was constant. However, there was a change in seasonal patterns in 2014. This research also use Mascon data from GRACE satellite to determine the radius of the gaussian filter and TRMM satellite’s data to observe rainfall data to be compared with the EWH variability from GRACE.

Seismic Retrofit of Concrete Buildings Damaged by Corrosion: A Case Study in Southern Italy

A case study of a building in southern Italy, subjected to high degradation by corrosion and waiting to be assessed for retrofit interventions, is presented. The owner required modifications to the building configuration, including a new layout of the floors and retrofitting for a high level of seismic load. A double strategy of an assessment and retrofit was carried out: dynamic linear and static non-linear analyses were performed, and the results were compared. Afterwards, a global strategy of mass and stiffness redistribution was implemented together with different retrofit interventions on the foundations, columns, and beams of the framed structure, such as reinforced concrete and steel jacketing, the application of FRP plates and fabrics, new steel elements, and steel–concrete composite floors. The results of the intervention are discussed and the implications of corrosion on the structures are explored. From the results obtained, it is possible to see how the use of different techniques for strengthening and passive seismic protection strategy can allow engineers to obtain the result of structural adaptation to earthquakes with low-cost interventions. The widespread adoption of steel jackets, coupled with the construction of floors using a steel–concrete composite structure, grants a good confinement of the beam–column r.c. joints, together with the overall strengthening of the existing structure. The adoption of CFRP wrapping at the lower edge of the beams implies a limited increase in thickness and the limited interventions of partial demolition from the existing structural members. A critical review of the steel jacketing aspects in terms of bending and shear strengthening is reported by considering this technique in the form of a steel exoskeleton containing the damaged concrete structure, by confining concrete elements, and by increasing the performance for both gravitational and seismic loads.

Prolonged pattern coarsening in ion irradiated swinging Si substrates

Low energy ion beams are regarded as an effective means to spatio-temporally pattern a wide variety of surfaces that exhibit intriguing motifs. In this work, an unconventional technique of surface patterning has been demonstrated by studying the topographical evolution of a Si(100) surface in the presence of azimuthal sample swinging (Δϕ=20∘) under 500 eV Ar+irradiation with a polar incidence angle of 67°. A prolonged wavelength coarsening is also noticed for the swinging sample. Ripple anticoarsening is observed for static sample at high fluences. The linear and nonlinear growth regimes are found to bear a direct consequence of this unconventional geometry. Furthermore, two-dimensional slope distributions of the surface morphologies clearly indicate reduced asymmetries in the surface structures. The present geometry brings in an additional factor of differential exposure and redeposition effects in addition to the near-surface mass redistribution, the former being inconceivable in the static configuration. This study establishes the role of this unconventional yet simple method to precisely modify and control surface asymmetries and influence regime shifts.

An Integrated Approach to Control the Penetration Depth of 3D-Printed Hollow Microneedles

A variety of hollow microneedle (HMN) designs has emerged for minimally invasive therapies and monitoring systems. In this study, a design change limiting the indentation depth of the (3D) printed custom microneedle assembly (circular array of five conical frusta with and without a stopper, aspect ratio = 1.875) fabricated using stereolithography has been experimentally validated and modeled in silico. The micro-indentation profiles generated in confined compression on 1 mm ± 0.073 mm alginate films enabled the generation of a Prony series, where displacement ranged from 100 to 250 µm. These constants were used as intrinsic properties simulating experimental ramp/release profiles. Puncture occurred on two distinct hydrogel formulations at the design depth of 150 µm and indentation rate of 0.1 mm/s characterized by a peak force of 3.5 N (H = 31 kPa) and 8.3 N (H = 36.5 kPa), respectively. Experimental and theoretical alignments for peak force trends were obtained when the printing resolution was simulated. Higher puncture force and uniformity inferred by the stopper was confirmed via microscopy and profilometry. Meanwhile, poroviscoelasticity characterization is required to distinguish mass loss vs. redistribution post-indentation through pycnometry. Results from this paper highlight the feasibility of insertion-depth control within the epidermis thickness for the first time in solid HMN literature.

Elucidation of active components and target mechanism in Jinqiancao granules for the treatment of prostatitis and benign prostatic hyperplasia

Ethnopharmacological relevanceProstatitis and benign prostatic hyperplasia (BPH) are inflammations of the prostate gland, which surrounds the urethra in males. Jinqiancao granules are a traditional Chinese medicine used to treat kidney stones and this medicine consists of four herbs: Desmodium styracifolium (Osbeck) Merr., Pyrrosia calvata (Baker) Ching, Plantago asiatica L. and stigma of Zea mays L. Aim of the studyWe hypothesized that Jinqiancao granules could be a potential therapy for prostatitis and BPH, and this work aimed to elucidate active compounds in Jinqiancao granules and their target mechanisms for the potential treatment of the two diseases. Materials and methodsJinqiancao granules were commercially available and purchased. Database-driven data mining and networking were utilized to establish a general correlation between Jinqiancao granules and the two diseases above. Ultra-performance liquid chromatography-mass spectrometry was used for compound separation and characterization. The characterized compounds were evaluated on four G-protein coupled receptors (GPCRs: GPR35, muscarinic acetylcholine receptor M3, alpha-1A adrenergic receptor α1A and cannabinoid receptor CB2). A dynamic mass redistribution technique was applied to evaluate compounds on four GPCRs. Nitric acid (NO) inhibition was tested on the macrophage cell line RAW264.7. Molecular docking was conducted on GPR35-active compounds and GPR35 crystal structure. Statistical analysis using GEO datasets was conducted. ResultsSeventy compounds were isolated and twelve showed GPCR activity. Three compounds showed potent GPR35 agonistic activity (EC50 < 10 μM) and the GPR35 agonism action of PAL-21 (Scutellarein) was reported for the first time. Docking results revealed that the GPR35-targeting compounds interacted at the key residues for the agonist-initiated activation of GPR35. Five compounds showed weak antagonistic activity on M3, which was confirmed to be a disease target by statistical analysis. Seventeen compounds showed NO inhibitory activity. Several compounds showed multi-target properties. An experiment-based network reflected a pharmacological relationship between Jinqiancao granules and the two diseases. ConclusionsThis study identified active compounds in Jinqiancao granules that have synergistic mechanisms, contributing to anti-inflammatory effects. The findings provide scientific evidence for the potential use of Jinqiancao granules as a treatment for prostatitis and BPH.

Extrapolating the projected potential of gravitational lens models: property-preserving degeneracies

ABSTRACT While gravitational lens inversion holds great promise to reveal the structure of the light-deflecting mass distribution, both light and dark, the existence of various kinds of degeneracies implies that care must be taken when interpreting the resulting lens models. This article illustrates how thinking in terms of the projected potential helps to gain insight into these matters. Additionally it is shown explicitly how, when starting from a discretized version of the projected potential of one particular lens model, the technique of quadratic programming can be used to create a multitude of equivalent lens models that preserve all or a subset of lens properties. This method is applied to a number of scenarios, showing the lack of grasp on the mass outside the strong lensing region, revisiting mass redistribution in between images, and applying this to a recent model of the SDSS J1004+4112 cluster, as well as illustrating the generalized mass sheet degeneracy and source-position transformation. In the case of J1004, we show that this mass redistribution did not succeed at completely eliminating a dark mass clump recovered by grale near one of the quasar images.