Marine Soil Research Articles

Engineering Properties of Marine Soils and Offshore Foundations

To reduce greenhouse gas emissions and enhance energy security, the role of renewable energy in the global energy structure is becoming increasingly significant [...]

Experimental Investigation of the Axial Load Capacity of Bilateral Helix-Stiffened Cement Mixing Piles in Soft Marine Soil

Bilateral helix-stiffened cement mixing piles (BHCMPs) are a new type of robust composite pile. In this paper, the influence of design parameters, such as the diameter of inner helix plates and the number and size of outer mixing helix plates, on the vertical bearing performance of the pile foundation and the behavior of the pile diameter are investigated through laboratory model tests. The results indicate that incorporating a reverse-mixing process in the pile formation of helix-stiffened cement mixing piles can enhance the overall ultimate bearing capacity and strengthen the soil-cement of the piles. In the case of the same double-blade inner drill pipe, for every 22 mm increase in the blades, its ultimate compressive load-bearing capacity was increased by 10% to 25%, and its pullout load-bearing capacity was increased by 15% to 45%. With further increases in the blades, the percentage increase in the compressive and pullout load-bearing capacity was reduced. With an increase in the number of external mixing blades, its compressive load capacity increased in the range of 5% to 20%, and the elevation load capacity increased in the range of 10% to 25%. Increasing the number of reverse-mixing helix plates on the outer casing enhances the soil-cement strength of the pile body by approximately 15–20% for each additional plate. The average strength of the pile-body soil-cement for each additional plate is estimated to be 1 to 1.4 times that of the previous set, indicating that increased mixing iterations can enhance the perimeter soil-cement strength of the pile. The average bond diameter of soil-cement piles with double-blade internal drilling rods could be seen from the diameter of the piles formed, which was 1.02 to 1.21 times the diameter of the blades.

Characterizing the spatial variability of marine soil properties with site-specific sparse data using a Bayesian data fusion approach

Characterizing the spatial variability of marine soil properties with site-specific sparse data using a Bayesian data fusion approach

Electrodeposition of calcareous cement from seawater in marine silica sands

The erosion of marine sediments is a pressing issue for coastal areas worldwide. Established methods to mitigate coastal erosion fail to provide lasting and sustainable solutions to protect marine ecosystems. Here we demonstrate the application of mild electrical stimulations to precipitate calcareous mineral binders from seawater in the pores of marine soils via electrodeposition, an alternative approach to mitigating coastal erosion. Results of electrochemical laboratory experiments unveil that the polymorphs, precipitation sites, intrusion mechanisms, and effects of electrodeposited minerals in marine sands vary as a function of the magnitude and duration of applied voltage, soil relative density, and electrolyte ionic concentration. Surprisingly, in addition to the precipitation of calcium carbonate and magnesium hydroxide, the formation of hydromagnesite is also observed due to electrically driven fluctuations in the local pH\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$${pH}$$\\end{document}. These electrodeposits lead to enhanced mechanical and hydraulic properties of the marine sands, indicating that electrodeposition routes could be developed to reinforce marine soils in coastal areas that more closely mimic natural systems.

Analytical approach for lateral dynamic responses of offshore wind turbines supported by cement-soil composite steel pipe pile embedded in gassy marine sediment layer

This study aims to clarify and gain an insight into the lateral dynamic response of offshore wind turbines (OWTs) supported by cement-soil composite steel (CCS) pipe pile embedded in gassy marine sediment layer by developing an analytical approach. In the proposed approach, the CCS pipe pile-supported OWT system is divided into three parts including the tower part, the seawater-immersed steel pipe pile part, and the embedded CCS pipe pile part. The lateral dynamic behaviours of the CCS pipe pile-supported OWT system have been determined and then validated by solving the analytical solution of lateral displacement for the above three parts. Numerical discussions are finally conducted to analysis the influence of some physical parameters on the lateral displacement and the first-order natural frequency of the CCS pipe pile-supported OWT system. The main findings can be summarized as: (i) the offshore deep cement-soil mixing (ODCM) pile is an effective reinforcement method to reduce the lateral vibration of conventional steel pipe pile-supported OWTs; (ii) the increase in tower height and seawater layer thickness will have a serious negative impact on the lateral dynamic response of CCS pipe pile-supported OWTs; (iii) the increase in gas saturation of gassy marine soil will lead to a negative influence on the maximum lateral displacement of CCS pipe pile-supported OWTs.

Ecofriendly biosynthesis of copper nanoparticles from novel marine S. rhizophila species for enhanced antibiofilm, antimicrobial and antioxidant potential

Marine microorganisms offer a promising avenue for the eco-friendly synthesis of nanoparticles due to their unique biochemical capabilities and adaptability to various environments. This study focuses on exploring the potential of a marine bacterial species, Stenotrophomonas rhizophila BGNAK1, for the synthesis of biocompatible copper nanoparticles and their application for hindering biofilms formed by monomicrobial species. The study begins with the isolation of the novel marine S. rhizophila species from marine soil samples collected from the West coast region of Kerala, India. The isolated strain is identified through 16S rRNA gene sequencing and confirmed to be S. rhizophila species. Biosynthesis of copper nanoparticles using S. rhizophila results in the formation of nanoparticles with size of range 10–50 nm. The nanoparticles exhibit a face-centered cubic crystal structure of copper, as confirmed by X-Ray Diffraction analysis. Furthermore, the synthesized nanoparticles display significant antimicrobial activity against various pathogenic bacteria and yeast. The highest inhibitory activity was against Staphylococcus aureus with a zone of 27 ± 1.00 mm and the least activity was against Pseudomonas aeruginosa with a zone of 22 ± 0.50 mm. The zone of inhibition against Candida albicans was 16 ± 0.60 mm. The antibiofilm activity against biofilm-forming clinical pathogens was evidenced by the antibiofilm assay and SEM images. Additionally, the copper nanoparticles exhibit antioxidant activity, as evidenced by their scavenging ability against DPPH, hydroxyl, nitric oxide, and superoxide radicals, as well as their reducing power in the FRAP assay. The study highlights the potential of the marine bacterium S. rhizophila BGNAK1 for the eco-friendly biosynthesis of copper nanoparticles with diverse applications. Synthesized nanoparticles exhibit promising antibiofilm, antimicrobial, and antioxidant properties, suggesting their potential utility in various fields such as medicine, wastewater treatment, and environmental remediation.

Cyclic loading experiments and numerical analysis of laterally loaded piles constructed in marine soil

This study aims to thoroughly analyze the lateral loads that impact tubular steel piles, which are extensively employed in the construction of coastal structures. The ASTM D 3966 standard was followed for conducting field tests on test piles installed at the Mersin International Port. The “time-displacement” and “load-displacement” curves were obtained from the cyclic loading test of the laterally loaded piles at the construction site. The finite elements models of the tests conducted on the construction site with the same parameters was built to perform numerical analysis. At the end of the analysis, it was determined that the numerical model's results were highly consistent with those obtained from the field test. After verifying the field experiments with numerical models, a parametric study was conducted. Parametric studies were conducted to compare the lateral displacements of tubular steel piles with variations in pile diameter, wall thickness, and load application height effect. The relationship between pile head displacements and these parameters appears to be almost linear. It is noteworthy that a change of approximately 10 percent in these parameters shows a correlation with changes of up to 3 percent in deformations.

Mid-infrared spectroscopy determines the provenance of coastal marine soils and their organic and inorganic carbon content

Vegetated coastal ecosystems (VCE), encompassing tidal marshes, mangroves, and seagrasses, serve as significant ‘blue’ carbon (C) sinks. Improving our understanding of VCE soils and their spatial and temporal dynamics is essential for conservation efforts. Conventional methods to characterise the dynamics and provenance of VCE soils and measure their total organic carbon (TOC) and inorganic carbon (TIC) contents are cumbersome and expensive.We recorded the mid-infrared (MIR) spectra and measured the TOC and TIC content of 323 subsamples across consistent depths from 106 soil core samples. Using the spectra of each VCE, we determined their mineral and organic composition by depth. We then used a regression tree algorithm, cubist, to model TOC and TIC contents. We rigorously validated the models to test their performance with a 10-fold cross-validation, bootstrapping, and a separate random test dataset.Our analysis revealed distinct mineralogical and organic MIR signatures in VCE soils that correlated with their position within the seascape. The spectra showed decreased clay minerals and increased quartz and carbonate with distance from freshwater inputs. The mineralogy of tidal marsh and mangrove soils differed with depth, showing larger absorptions due to carbonate and quartz and weakening clay minerals and organics absorptions. The mineralogy of the seagrass soils remained the same with depth. The cubist models to estimate TOC and TIC content were accurate (Lin's concordance correlation, ρc≥ 0.92 and 0.93 respectively) and interpretable, confirming our understanding of C in these systems. These findings shed light on the provenance of the soils and help quantify the flux and accumulation of TOC and TIC, which is crucial for informing VCE conservation. Moreover, our results indicate that MIR spectroscopy could help scale the measurements cost-effectively, for example, in carbon crediting schemes and to improve inventories. The approach will help advance blue C science and contribute to the conservation and protection of VCE.

A Novel Method for Estimating the Undrained Shear Strength of Marine Soil Based on CPTU Tests

The undrained shear strength is an essential parameter in the foundation design of marine structures. Due to the complex marine environment and technical limitations, it is difficult and costly to obtain offshore samples. Piezocone penetration tests (CPTU) are relatively low-cost compared to drilling and sampling methods. Therefore, based on the soil behavior type index (Ic) derived from CPTU results, a model for estimating cone factors (Nkt, Nke) is proposed to improve the accuracy of estimation of undrained shear strength. The result shows that the soil behavior type index (Ic) and cone factors take on a negatively correlated exponential relation. Incorporating a cone factor that varies with the soil behavior type index (Ic) significantly enhances the accuracy of undrained shear strength predictions compared to the conventional method of using a constant cone factor. This approach reduces the root mean square error (RMSE) for Nkt (Nke) from 0.124 (0.126) MPa to 0.056 (0.06) MPa, and the mean absolute error (MAE) from 0.0154 (0.016) MPa to 0.0032 (0.0036) MPa. The method was validated at an additional location and the predictions were in high agreement with the results of the consolidated quick direct shear test. The developed method can serve as an effective tool used in the design of foundations of marine structures.

Comprehensive analysis of cement-stabilised dredged marine soil: Evaluating physical, microstructural, and mechanical characteristics

This study evaluates the index, microstructural, and mechanical behaviour of cement-stabilised soft soil. The marine soil dredged from Hangzhou is taken for the study. Different percentages of ordinary Portland cement (OPC)by dry mass were added to the soils during reconstitution, i.e., 5, 10, 15, and 20%. The reconstituted samples were analysed in the laboratory to determine basic properties such as moisture content, void ratio, density, specific gravity, consistency limits, and particle size distribution. The microstructures of the reconstituted samples were also evaluated through scanning electron microscopy. Results inferred a significant impact on the physical properties of the soil. An increase in cement content increases the consistency limits. Additionally, the specific gravity, plasticity index, and density of the treated soil initially decreased but increased upon further addition of cement content. The microstructure of the soil transformed from a dispersed to a flocculated structure, with larger and denser particles. The change in microstructure was also evident in the particle size distribution, with an increase in cement content leading to larger size particles. Finally, the unconfined compression test conducted on the reconstituted soil indicates a substantial enhancement in strength with higher cement content and prolonged curing periods.

Investigation on solidified/stabilized behavior of marine soil slurry by lime-activated incinerated sewage sludge ash-ground granulated blast furnace slag under multifactor conditions

This study aims to evaluate the possibility of reusing treated marine clayey soils by stabilization/solidification (S/S) technology as geomaterial in reclamation projects from the aspects of engineering strength, chemical modification and environmental risk assessment. The lime-activated incinerated sewage sludge ash (ISSA) together with ground granulated blast furnace slag (GGBS) was employed as the binder. The multi-controlling factors including water content, curing time, salinity, and chemical compositions of mixing solution were taken account to identify the S/S treated Hong Kong marine deposit (HKMD) slurry based on the strength tests, pH measurement, thermo-gravimetric (TG) analysis, X-ray diffractometer (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy coupled with energy-dispersive spectrometry (SEM-EDS) and toxicity characteristic leaching procedure (TCLP) tests, etc. The results show that the S/S treatment using lime-activated ISSA-GGBS can effectively enhance the strength of marine soil at initial water content of 110% and 200%. The water content and curing time have a significant impact on the S/S treated HKMD. The pH of treated soils is higher than 11.1, which proves an alkaline environment for the reactions in the treated soil. A special case is the treated HKMD at 200% water content hydrated by MgCl2 solution, which has a low pH of 10.23 and maintains a slurry state. Based on the TCLP results, the leaching concentration of heavy metals from S/S treated HKMD is environmentally safe and meets Hong Kong standard for reusing treated soil with a low level of <0.2 mg/L. The content of main products such as calcium/magnesium silicate hydrate, ettringite or Friedel's salt depends on the chemical additions (e.g. distilled water, seawater, NaCl and Na2SO4). The products in the specimens mixed with MgCl2 solutions are mainly composed of Mg(OH)2, M-S-H and MgCO3, which is distinct with the neoformations in the other cases. Therefore, this study proves that the S/S treated soil slurry could be reused as geomaterials in reclamation projects, and the S/S process is greatly affected by water content, curing time and solution compositions, etc.

Microbial Diversity and Nitrogen Cycling in Peat and Marine Soils: A Review

Nitrogen is an essential nutrient for living organisms in peat and marine soils, and its transformation within the soil matrix is a complex process mediated by various microbes that inhabit these ecological niches. The metabolism of nitrogen is governed by microbially mediated biogeochemical transformations, such as nitrification, anammox, and denitrification, which contribute to the assimilated pool of nitrogen and fixed nitrogen loss. One of the major challenges facing the field of peat and marine microbiology is the lack of understanding of the correlation between ecosystem-driven nitrogen transformation and microbial diversity. This is crucial because of growing concerns regarding the impacts of human-induced activities and global climate change on microbial nitrogen-cycling processes in peat and marine soils. Thus, this review aimed to provide a comprehensive overview of the current understanding of the microbial communities involved in peat and marine nitrification, anammox, and denitrification; the factors influencing the niche differentiation and distribution of the main functional components; the genes involved; and the main effects of human-induced activities and global climate change on the peat and marine nitrogen cycle. The implications of this review will facilitate an understanding of the complex mechanisms associated with ecosystem function in relation to nitrogen cycling, the role of peat and marine soils as carbon sinks, pollution remediation using naturally occurring populations of diverse microbes, and the development of policies to mitigate the effects of anthropogenic influences in peat and marine soils.

Irrigation Practices and Their Effects on Soil Quality and Soil Characteristics in Arid Lands: A Comprehensive Geomatic Analysis

Comprehension of the long-term effects of irrigation on basic soil characteristics and quality is essential for sustainable land management and agricultural production, particularly in arid regions where water availability is limited. This study aimed to investigate long-term irrigation effects on soil quality, soil organic carbon (SOC), and nitrogen (N) stocks in the arid lands of Egypt. Seventy soil samples were collected and analyzed to determine various soil properties. A soil quality index (SQI), SOC, and N stocks were computed. ANOVA and PCA analyses were used to identify significant differences between alluvial soils in the southwest part of the investigated area and coastal marine soils in the northeast of the study area. The results demonstrated that most of the studied soil parameters had significantly greater values in alluvial compared to coastal marine soils. Long-term irrigation led to an 8.00% increase in SOC and 7.22% increase in N stocks compared to coastal marine soils production. Furthermore, a 39.53% increase was found in the SQI upon long-term irrigation practice. These results suggest that shifting from rain-fed in coastal marine areas to irrigated production systems in alluvial fields can improve soil quality, SOC, and N stocks. Therefore, further studies are required to investigate the impact of additional factors, such as irrigation method and salinity status of sub-surface soil layers, to enhance agricultural productivity and sustainable land use.

Secondary metabolite profiling using HR-LCMS, antioxidant and anticancer activity of Bacillus cereus PSMS6 methanolic extract: In silico and in vitro study

Novel anticancer drugs of natural origin have increased tremendously due to the resistance of multiple chemotherapeutic drugs in breast cancer therapy and their high toxicity with undesirable side effects. The study investigates the bioactivity of secondary metabolites derived from Bacillus cereus PSMS6 isolated from marine soil sediment in the Velar estuary, Parangepattai, Cuddalore district, Tamil Nadu, and India. Strains were isolated and antagonistic activity was screened using the agar well diffusion method. B. cereus PSMS6 exhibited potency, and its crude extract was tested for antioxidant, anticancer, and cytotoxic MTT assay potential. The methanolic extract of B. cereus PSMS6 was analyzed by mass spectrometry HRLC-MS and FT-IR to determine the bioactive compounds. A drug interaction study with the anti-breast cancer protein HER2 was performed by molecular docking analysis. Antioxidant activities were determined using total antioxidant scavenging assay, ABTS and DPPH free radical scavenging assays. The total antioxidant scavenging assay of the crude extract of B. cereus methanol had an IC50 value of 28.33±1.01, in ABTS IC50 value of the extract was 29.00±0.28 and in DPPH the IC50 of the extract was 34.91±0.09. The negative ion compound Palmitoylglycerone phosphate had a LibDock score of 149.487 and the positive ion compound N5-(4-Methoxybenzyl) glutamine had 120.116. These compounds show promising anticancer activity. The current study reported that the bioactive secondary metabolite of B. cereus PSMS6 retains anti-cancer, and antioxidant properties. This is the first report to show the production of the Palmitoylglycerone phosphate metabolite from B. cereus PSMS6.

Study on the anisotropic small strain stiffness of marine clay in China using bender elements

Small strain stiffness anisotropy of marine soils plays an important role in determining the natural frequency of offshore wind turbine structures. The small strain stiffness anisotropy is contingent on stress anisotropy and fabric anisotropy, with the latter being assessable via stiffness anisotropy under isotropic stress conditions. In this study, the small strain stiffness of marine clay obtained from the site of an offshore wind farm on the southeast coast of China was measured using a bender element. The effects of void ratio, effective mean pressure, and overconsolidation ratio on small strain stiffness were investigated and the related empirical equations were proposed for predicting the small strain stiffness of the marine clay. Furthermore, the small strain stiffness anisotropy of the marine clay under isotropic consolidation was also investigated. The findings revealed remarkable anisotropy in the small strain stiffness of the tested marine clay.

Characterization and Antibacterial Activity of Melanin Pigment from Marine Bacterium Actinoalloteichus cyanogriseus

Introduction: Marine microbes are known to produce novel bioactive compounds. In this work, the bacterium Actinoalloteichus cyanogriseus, AU-RM-4, isolated from marine soil sediment, capable of producing high amounts of black extracellular melanin pigment when grown on anaerobic actinomycetes agar is reported. The maximum melanin production was approximately 2.5 mg/mL. Methods: The melanin pigment was recovered using acid treatment and the characteristic melanin absorption peaks at 253 nm and 291 nm were observed in the UV range. The antibacterial activity of the melanin pigment against a range of clinically infectious bacteria was assayed in vitro by disc diffusion and serial dilution methods. Results: The A. cyanogriseus melanin showed antimicrobial activity against Staphylococcus aureus and Klebsiella pneumoniae (MIC value -500 μg/mL). The effects of the AU-RM-4 melanin on the morphology of pathogenic bacteria were observed under scanning (SEM) and transmission electron microscopy (TEM). It is concluded that A. cyanogriseus isolate AU-RM-4 is a promising melanin-producing marine bacterium, whose melanin pigment has antibacterial activity against certain pathogenic bacteria (S. aureus and K. pneumoniae). Conclusion: Melanin produced by A. cyanogriseus AU-RM-4 could have commercial potential in the pharmaceutical and cosmetic industries for the production of pharmaceutical formulations, and also in the food industries as a colouring and antibacterial agent.

Production, optimization and characterization of partially purified anti-mycotic compound from marine soil derived streptomycetes originating at unexplored region of Bay of Bengal, India

Sixty-eight morphologically distinct isolates of marine actinomycetes were derived from seashore, mangrove, and saltpan ecosystems located between the Palk Strait and Gulf of Mannar region, Bay of Bengal, Tamilnadu. Twenty-five (36.8%) isolates exhibited anti-mycotic activity against Candida albicans and Cryptococcus neoformans in preliminary screening, and 4 isolates with prominent activity were identified and designated at the genus level as Streptomyces sp. VPTS3-I, Streptomyces sp. VPTS3-2, Streptomyces sp. VPTSA1-4 and Streptomyces sp. VPTSA1-8. All the potential antagonistic isolates were further characterized with phenotypic and genotypic properties including 16S rRNA gene sequencing and identified species level as Streptomyces afghaniensis VPTS3-1, S. matensis VPTS3-2, S. tuirus VPTSA1-4 and S. griseus VPTSA1-8. In addition, the active fractions from the potential antagonistic streptomycetes were extracted with organic solvents by shake flask culture method and the anti-mycotic efficacies were evaluated. The optimization parameters for the production of the anti-mycotic compound were found to be pH between 7 and 8, the temperature at 30ᵒC, the salinity of 2%, incubation of 9 days, and starch and KNO3 as the suitable carbon and nitrogen sources respectively in starch casein medium.

Capillary water absorption and strength of solidified marine soft soil

In order to reveal the capillary water absorption properties and durability of soft soil solidified with industrial wastes, soda residue (SR), ground granulated blast furnace slag (GGBS), and carbide slag (CS) were used as solidifiers to solidify marine soil. The effects of solidifier content and water absorption path on capillary water absorption properties, strength, and microscopic mechanism were investigated through capillary water absorption, unconfined compressive strength (UCS), X-ray diffraction, and nuclear magnetic resonance tests. And the properties were compared with cement solidified soil. Results showed that capillary absorption parameters, including sorptivity coefficient, capillary coefficient, and diffusion coefficient, decreased with solidifier content and increased with water absorption times. The capillary absorption parameters of SR-GGBS-CS solidified soil were lower than those of cement solidified soil. For samples with 28d-UCS greater than 1390.9 kPa, the UCS after capillary absorption tended to increase up to 1.28 times. With increasing water absorption time, NaCl migrated upward and accumulated, accompanied by the change of hydrated products such as calcium chloroaluminate hydrate and ettringite, which contributed to a decrease in the strength of solidified soil. The carbon emission index of cement solidified soil was approximately 41 times that of SR-GGBS-CS solidified soil, and the soil with SR, GGBS, and CS content of 25%, 10%, and 4% respectively had the lowest carbon emission index.

A review on drainage of dredged marine soils: Advances and prospects

Drainage is a common practice in geotechnical engineering concerning dredged marine soils. Current drainage techniques, including surcharge preloading, vacuum preloading, and combined vacuum-surcharge preloading, have been proven to be effective in soft soil treatment, but are also criticized for their high energy consumption. This paper made a brief review on existing drainage techniques and proposed some prospects for the next-generation techniques in response to the public concern of sustainability. It is found that all conventional preloading techniques have been well studied from tests to modeling, and improved vacuum preloading tends to be used in combination with other techniques. Drainage techniques with lower energy consumption can be realized either by using renewable energy or designing biomimetic devices. The paper is expected to provide a comprehensive while concise report on recent advances in drainage techniques for dredged marine soils and in the meanwhile give an insight into the further development towards a more sustainable future.

Improvement on Strength, Durability, and Crack Closure Behavior of Bacteria Concrete under Marine Soil Exposures

Abstract In the concrete industry, bacteria-precipitated calcium carbonate (CaCO3) can be used to repair cracks. If cracks in concrete are not repaired in a timely manner, other durability problems arise, necessitating expensive crack repair methods. Bacteria can be isolated from an appropriate physical environment so that they continue to function effectively in concrete. In this study, suitable self-healing bacteria are isolated from a concrete dump yard and incorporated into concrete specimens. After 3 days of normal curing, the specimens were pre-cracked and kept in marine exposure conditions for a period of 56 days. Strength and durability properties such as compressive strength regain, ultrasonic pulse velocity (UPV), and dynamic Young’s modulus were studied, and a water absorption test was carried out for cubes exposed in marine soil conditions. Marine soil treated with a bacteria and nutrient solution (1:9) exhibited improved crack-healing behavior as well as increased strength and durability. Compressive strength regain of 45.98 %, an increased modulus of 54.04 %, and crack healing of 83.05 % were reached with a 60 % reduction in water absorption and 32.49 % increment in UPV values. A microstructure study demonstrates the presence of CaCO3 compounds in healed crack surfaces and treated marine soil, which is primarily due to bacterial action under marine exposures conditions.