Marine Mussel Species Research Articles

Microeukaryotes Associated with Freshwater Mussels in Rivers of the Southeastern United States.

Microeukaryotes are a diverse and often overlooked group of microbes that are important in food webs and other ecological linkages. Little is known about microeukaryotes associated with aquatic invertebrates, although filter feeders such as mussels are likely to take in and potentially retain microeukaryotes in their gut while feeding. Microeukaryotes such as apicomplexans have been reported in marine mussel species, but no studies have examined the presence of these microorganisms in freshwater mussels or how they relate to mussel host species or environmental conditions. In this study, microbial community DNA was extracted from the gut tissue of over 300 freshwater mussels, representing 22 species collected from rivers in the southeastern USA. Microeukaryote DNA was detected using PCR amplification, followed by the sequencing of positive amplicons. Microeukaryotes were found in 167 individual mussels (53%) of those tested. Amplicons included dinoflagellates/algae that differed between mussel species and are likely food sources that were distinct from those found in water and sediment samples analyzed concurrently. A total of 5% of the positive amplicons were non-photosynthetic alveolates that could represent parasitic microeukaryotes. Understanding the distribution of microeukaryotes in the freshwater mussel gut microbiome could further our understanding of the ongoing decline of mussel populations.

Patterns of admixture and introgression in a mosaic Mytilus galloprovincialis and Mytilus edulis hybrid zone in SW England.

The study of hybrid zones offers important insights into speciation. Earlier studies on hybrid populations of the marine mussel species Mytilus edulis and Mytilus galloprovincialis in SW England provided evidence of admixture but were constrained by the limited number of molecular markers available. We use 57 ancestry-informative SNPs, most of which have been mapped genetically, to provide evidence of distinctive differences between admixed populations in SW England and asymmetrical introgression from M. edulis to M. galloprovincialis. We combine the genetic study with analysis of phenotypic traits of potential ecological and adaptive significance. We demonstrate that hybrid individuals have brown mantle edges unlike the white or purple in the parental species, suggesting allelic or non-allelic genomic interactions. We report differences in gonad development stage between the species consistent with a prezygotic barrier between the species. By incorporating results from publications dating back to 1980, we confirm the long-term stability of the hybrid zone despite higher viability of M. galloprovincialis. This stability coincides with a dramatic change in temperature of UK coastal waters and suggests that these hybrid populations might be resisting the effects of global warming. However, a single SNP locus associated with the Notch transmembrane signalling protein shows a markedly different pattern of variation to the others and might be associated with adaptation of M. galloprovincialis to colder northern temperatures.

Molecular diet analysis in mussels and other metazoan filter feeders and an assessment of their utility as natural eDNA samplers.

Molecular gut content analysis is a popular tool to study food web interactions and has recently been suggested as an alternative source for DNA-based biomonitoring. However, the overabundant consumer's DNA often outcompetes that of its diet during PCR. Lineage-specific primers are an efficient means to reduce consumer amplification while retaining broad specificity for dietary taxa. Here, we designed an amplicon sequencing assay to monitor the eukaryotic diet of mussels and other metazoan filter feeders and explore the utility of mussels as natural eDNA samplers to monitor planktonic communities. We designed several lineage-specific rDNA primers with broad taxonomic suitability for eukaryotes. The primers were tested using DNA extracts of different limnic and marine mussel species and the results compared to eDNA water samples collected next to the mussel colonies. In addition, we analysed several 25-year time series samples of mussels from German rivers. Our primer sets efficiently prevent the amplification of mussels and other metazoans. The recovered DNA reflects a broad dietary preference across the eukaryotic tree of life and considerable taxonomic overlap with filtered water samples. We also show the utility of a reversed version of our primers, which prevents amplification of nonmetazoan taxa from complex eukaryote community samples, by enriching fauna associated with the marine brown algae Fucus vesiculosus. Our protocol will enable large-scale dietary analysis in metazoan filter feeders, facilitate aquatic food web analysis and allow surveying of aquacultures for pathogens. Moreover, we show that mussels and other aquatic filter feeders can serve as complementary DNA source for biomonitoring.

An ecotoxicological approach for assessing marine pollution: Comparative study of multi-responses of marine mussels, Mytilus galloprovincialis and Perna perna, exposed to pollutant heavy metals (copper and lead)

The present research aims to evaluate the variation of several responses such as nitrogen and phosphorus excretion, catalase activity and total protein in the two species of marine mussels, Mytilus galloprovincialis (Ain Tagoureite-Tipaza, Algeria) and Perna perna (Figuier-Boumerdes, Algeria), exposed to different sub-lethal concentrations of two heavy metals (copper, and lead). The measurement of the chemical and biochemical responses were made after an acute exposure period (the mussels’ size is between 4.5 and 5.5 cm). The results showed that, through all exposure cycles, variations in temperature and salinity [0.10±0.05°C > ΔT > 1.6±0.13°C, 0.05 ± 0.02‰ > ΔS > 0.65 ± 0.03‰] are far from significant in order to interfere with the effect caused by copper or lead pollutant. In both species, the remarkable changes in nitrogen and phosphorus mineral matter under two metals effect are a physiological disturbance origin. Consequently, the catalase induction and the proteins depletion [Mytilus galloprovincialis: |−0.138| > Δ[protein total] > |−1.601| (mg g−1 of flesh), Perna perna: |−1.046| > Δ[protein total] > (0.56 mg g−1 of flesh)] during the contamination cycles reflected the oxidative stress caused with the varying levels. However, in decontamination cycles, the catalase enzymatic activity [Mytilus galloprovincialis ([Cu]=0.100 mg/l): [CAT]day1=18.4, [CAT]day3=50.06, and [CAT]day11=29.3 U mg −1 of proteins, Perna perna([Cu] = 0.100 mg/l): [CAT]day1=19.4, [CAT]day3=59.11, and [CAT]day11=33.02 U mg −1 of proteins], has revealed the normal state return when the environment is free of contamination. Through these results, the good relevance of pollution indication in the two species depends essentially on their sensitivity and tolerance, causing physiological and/or metabolite responses will vary according to the xenobiotics and their dose.

Toxicity of two drugs towards the marine filter feeder Mytilus spp, using biochemical and shell integrity parameters

The increasing presence of anthropogenic contaminants in the environment may constitute a challenge to non-target biota, considering that most contaminants can exert deleterious effects. Salicylic acid (SA) is a non-steroid anti-inflammatory drug (NSAID) which exerts its activity by inhibiting the enzyme cyclooxygenase (COX). Another class of drugs is that of the diuretics, in which acetazolamide (ACZ) is included. This pharmaceutical acts by inhibiting carbonic anhydrase (CA), a key enzyme in acid-base homeostasis, regulation of pH, being also responsible for the bio-availability of Ca2+ for shell biomineralization processes. In this work, we evaluated the chronic (28-day) ecotoxicological effects resulting from the exposures to SA and ACZ (alone, and in combination) on individuals of the marine mussel species Mytillus spp., using enzymatic (catalase (CAT), glutathione S-transferases (GSTs), COX and CA), non-enzymatic (lipid peroxidation, TBARS levels) and morphological and physiological (shell hardness, shell index and feeding behaviour) biomarkers. Exposure to ACZ and SA did not cause significant alterations in CAT and GSTs activities, and in TBARS levels. In terms of CA, this enzyme was inhibited by the highest concentration of ACZ in gills of exposed animals, but no effects occurred in the mantle tissue. The activity of COX was not altered after exposure to the single chemicals. However, animals exposed to the mixture of ACZ and SA evidenced a significant inhibition of COX activity. Morphological and physiological processes (namely, feeding, shell index, and shell hardness) were not affected by the here tested pharmaceutical drugs. Considering the general absence of adverse effects, further studies are needed to fully evaluate the effects of these pharmaceutical drugs on alternative biochemical and physiological pathways.

Measuring Metal Uptake and Loss in Individual Organisms: A Novel Double Stable Isotope Method and its Application in Explaining Body Size Effects on Cadmium Concentration in Mussels.

Interindividual variabilities in metal bioaccumulation confound our interpretation of the biomonitoring data. Measuring metal toxicokinetics in organism "individuals" may provide insights into the processes underlying the variabilities. Therefore, we developed a double stable isotope method that can simultaneously measure uptake and elimination of metals in individual organisms and thus the distribution of the toxicokinetic parameters. Specifically, we exposed organisms to both isotopes (113Cd and 114Cd; Cd = cadmium) during the first stage and to only one isotope (114Cd) during the second stage. Metal uptake and elimination rate constants (i.e., ku and ke) were simultaneously estimated from the content of the two isotopes measured in each organism at the end of the second stage. We applied the method to investigate the interindividual variability in Cd concentrations caused by body size in two marine mussel species. Cd concentrations are higher in larger Xenostrobus atratus but lower in smaller Perna viridis. Size-dependent Cd uptake is found to be responsible for size effects on Cd concentrations in the mussels and the interspecies differences in the relationship between Cd concentration and body size. Specifically, Cd ku increases with size in X. atratus (0.057-0.297 L g-1 d-1) but decreases with size in P. viridis (0.155-0.351 L g-1 d-1). In contrast, Cd ke is not influenced by body size (X. atratus: 0.002-0.060 d-1; P. viridis: 0.008-0.060 d-1). Overall, we extended the applicability of the stable isotope methods to measure metal toxicokinetics in "individual" organisms, providing a readily available tool for investigating problems related to metal bioaccumulation.

Proteomic analysis and biochemical alterations in marine mussel gills after exposure to the organophosphate flame retardant TDCPP

Organophosphate flame retardants (OPFRs) are (re-)emergent environmental pollutants increasingly being used because of the restriction of other flame retardants. The chlorinated OPFR, tris(1,3-dichloro-2-propyl) phosphate (TDCPP) is among those of highest environmental concern, but its potential effects in the marine environment have rarely been investigated. We exposed a widely used sentinel marine mussel species, Mytilus galloprovincialis, to 10 μg L−1 of TDCPP during 28 days and studied: (i) the kinetics of bioaccumulation and elimination of the compound, (ii) the effect on two molecular biomarkers, glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities, and (iii) proteomic alterations in the gills, following an isobaric labeling quantitative shotgun proteomic approach, at two exposure times (7 and 28 days). Uptake and elimination of TDCPP by mussels were very fast, and the bioconcentration factor of this compound in mussels was 147 L kgww-1, confirming that this compound is not very bioaccumulative, as predicted by its chemical properties. GST activity was not affected by TDCPP exposure, but AChE activity was inhibited by TDCPP at both 7 and 28 days of exposure. Proteomic analysis revealed subtle effects of TDCPP in mussel gills, since few proteins (less than 2 % of the analysed proteome) were significantly affected by TDCPP, and effect sizes were low. The most relevant effects detected were the up-regulation of epimerase family protein SDR39U1, an enzyme that could be involved in detoxification processes, at both exposure times, and the down-regulation of receptor-type tyrosine-protein phosphatase N2-like (PTPRN2) after 7 days of exposure, which is involved in neurotransmitter secretion and might be related to the neurotoxicity described for this compound. Exposure time rather than TDCPP exposure was the most important driver of protein abundance changes, with 33 % of the proteome being affected by this factor, suggesting that stress caused by laboratory conditions could be an important confounding factor that needs to be controlled in similar ecotoxicology studies. Proteomic data are available via ProteomeXchange with identifier PXD019720.

Mercury in Marine Mussels from the St. Lawrence Estuary and Gulf (Canada): A Mussel Watch Survey Revisited after 40 Years

Various species of marine mussels have been used, in the last 50 years, as sentinel organisms for monitoring metal contamination along marine coasts. There are two main reasons for this: these mollusks concentrate metals in their soft tissue and they are geographically widespread. In practice, trace metal concentrations in mussel soft tissue reveal (after some correction for biotic effects) the contamination level of their surrounding environment. We present the results of a mercury (Hg) survey in Mytilus spp. collected in the summers of 2016, 2018, and 2019 at 51 stations distributed along the coasts of the Estuary and Gulf of St. Lawrence. Mercury concentrations ranged from 0.063 to 0.507 µg g−1 (dry weight, dw), with a grand mean of 0.173 ± 0.076 µg g−1 dw (±1 standard deviation), and a median of 0.156 µg g−1 dw for the 504 individuals analyzed. Mercury contents per individual mussel were significantly (p < 0.01) related to shell length and dry tissue weight, with the smaller individuals having the highest Hg concentrations. To take into account these biotic effects, we normalized Hg concentrations of the mussel soft tissue for constant shell length (L) and soft tissue weight (TW) based on the log-log relationships between Hg content and L or TW. The normalized Hg contents of mussels varied from 10.9 to 66.6 ng per virtual individual of 35 mm length and 0.17 g dry weight. A similar normalization procedure applied to 1977–1979 data, yielded a very similar range: 12 to 64 ng. This observation suggests that the Hg bioavailable to marine mussels in the study area did not change over a span of 40 years. Regional Hg distribution patterns indicate a gradual decrease of Hg content in mussels downstream from freshwater discharges to the St. Lawrence Estuary and the Baie des Chaleurs, suggesting that rivers constitute a significant Hg source in these estuarine systems. Atmospheric Hg deposition and concentration in marine waters of the Atlantic Ocean are known to have decreased in the last decades. However, in coastal environments, the response to these changes does not seem to be rapid, probably because of the long residence time of Hg in soils before being exported to coastal areas.

How do species barriers decay? Concordance and local introgression in mosaic hybrid zones of mussels.

The Mytilus complex of marine mussel species forms a mosaic of hybrid zones, found across temperate regions of the globe. This allows us to study 'replicated' instances of secondary contact between closely related species. Previous work on this complex has shown that local introgression is both widespread and highly heterogeneous, and has identified SNPs that are outliers of differentiation between lineages. Here, we developed an ancestry-informative panel of such SNPs. We then compared their frequencies in newly sampled populations, including samples from within the hybrid zones, and parental populations at different distances from the contact. Results show that close to the hybrid zones, some outlier loci are near to fixation for the heterospecific allele, suggesting enhanced local introgression, or the local sweep of a shared ancestral allele. Conversely, genomic cline analyses, treating local parental populations as the reference, reveal a globally high concordance among loci, albeit with a few signals of asymmetric introgression. Enhanced local introgression at specific loci is consistent with the early transfer of adaptive variants after contact, possibly including asymmetric bi-stable variants (Dobzhansky-Muller incompatibilities), or haplotypes loaded with fewer deleterious mutations. Having escaped one barrier, however, these variants can be trapped or delayed at the next barrier, confining the introgression locally. These results shed light on the decay of species barriers during phases of contact.

Effects of chronic exposure to sediments from the Zarzis area, Gulf of Gabes, measured in the mussel (Mytilus spp.): a multi-biomarker approach involving oxidative stress and neurotoxicity

ABSTRACT The hereby study was conducted to assess the chronic biological effects of potentially contaminated sediments collected at the Zarzis area, Tunisia, on the marine mussel species (Mytilus spp.). Useful information can be provided through the analysis of biomarkers that allow inferring about the presence of pollutants and their adverse effects, contributing for a better assessment of ecosystems' quality and functions. Three sampling sites were chosen taking into account the putative impact of different human activities at several sites located in Zarzis area, compared to an unpolluted reference site, located in Afurada, Vila Nova de Gaia, Portugal. Physical-chemical characterization involved the survey of metals (Cd, Cu, Cr, Hg, Zn and Pb) and polycyclic aromatic hydrocarbons (PAHs), whose toxic effects are involved in the onset of oxidative stress. Biomarkers involving oxidative stress and neurotoxicity were quantified in hemolymph, gills, and digestive gland tissues of Mytilus spp. following the chronic exposure to the distinct sediments. Organisms exposed to sediments collected at the Zarzis area presented the highest values of the tested oxidative stress biomarkers and a significant inhibition of AChE activity in comparison with the unpolluted reference site. This set of results suggests that biomarker responses representing different biological endpoints in Mytilus spp. are useful for the assessment of environmental pollution effects, namely by PAHs and metals. Our results suggest the presence of these chemicals in specific sampling sites whose sediment caused evident biological responses in mussels.

Seasonal distribution of cadmium, lead, nickel, and magnesium in several tissues of mussels from the Algerian coasts.

This study aims to investigate the distribution of heavy metals (Cd, Pb, Ni, and Mn) in different organs of two marine mussel species, Perna perna collected from Figuier site and Mytilus galloprovincialis sampled from Sercouf and Algiers port sites of the Algerian coast. Thirty individuals (>5cm length) were seasonally sampled over a 1-year period from each site, and condition index (CI) and gonado somatic index (GSI) of mussels were calculated. The gill, digestive gland, rest of soft tissues, and gonad were accurately isolated and heavy metal contents were analyzed by ICP-MS. Heavy metal results, expressed on μg/g dry weight, ranged from 0.1 to 2.6 Cd, 0.1 to 17.15 Pb, 0.36 to 25.7 Ni, and 3.68 to 74.76 Mn. Thus, typical distribution of studied metals in various organs of mussels was found. In fact, the digestive gland followed by gill revealed significantly high metal concentrations. However, gonad showed low heavy metal contents. Different patterns of heavy metal distribution in various organs of mussels were found at the three sampling sites. High levels of Cd, Pb, Ni, and Mn were detected in mussels of Sercouf, Algiers port, and Figuier sites, respectively. According to the Algerian and European norms, mussels collected from all sites were healthy except those of Algiers port sampled in autumn and winter seasons where lead contamination was found.

High-throughput quantification of protein structural change reveals potential mechanisms of temperature adaptation in Mytilus mussels

BackgroundTemperature exerts a strong influence on protein evolution: species living in thermally distinct environments often exhibit adaptive differences in protein structure and function. However, previous research on protein temperature adaptation has focused on small numbers of proteins and on proteins adapted to extreme temperatures. Consequently, less is known about the types and quantity of evolutionary change that occurs to proteins when organisms adapt to small shifts in environmental temperature. In this study, these uncertainties were addressed by developing software that enabled comparison of structural changes associated with temperature adaptation (hydrogen bonding, salt bridge formation, and amino acid use) among large numbers of proteins from warm- and cold-adapted species of marine mussels, Mytilus galloprovincialis and Mytilus trossulus, respectively.ResultsSmall differences in habitat temperature that characterize the evolutionary history of Mytilus mussels were sufficient to cause protein structural changes consistent with temperature adaptation. Hydrogen bonds and salt bridges that increase stability and protect against heat-induced denaturation were more abundant in proteins from warm-adapted M. galloprovincialis compared with proteins from cold-adapted M. trossulus. These structural changes were related to deviations in the use of polar and charged amino acids that facilitate formation of hydrogen bonds and salt bridges within proteins, respectively. Enzymes, in particular those within antioxidant and cell death pathways, were over-represented among proteins with the most hydrogen bonds and salt bridges in warm-adapted M. galloprovincialis. Unlike extremophile proteins, temperature adaptation in Mytilus proteins did not involve substantial changes in the number of hydrophobic or large volume amino acids, nor in the content of glycine or proline.ConclusionsSmall shifts in organism temperature tolerance, such as that needed to cope with climate warming, may result from structural and functional changes to a small percentage of the proteome. Proteins in which function is dependent on large conformational change, notably enzymes, may be particularly sensitive to temperature perturbation and represent foci for natural selection. Protein temperature adaptation can occur through different types and frequencies of structural change, and adaptive mechanisms used to cope with small shifts in habitat temperature appear different from mechanisms used to retain protein function at temperature extremes.

A single clonal lineage of transmissible cancer identified in two marine mussel species in South America and Europe.

Transmissible cancers, in which cancer cells themselves act as an infectious agent, have been identified in Tasmanian devils, dogs, and four bivalves. We investigated a disseminated neoplasia affecting geographically distant populations of two species of mussels (Mytilus chilensis in South America and M. edulis in Europe). Sequencing alleles from four loci (two nuclear and two mitochondrial) provided evidence of transmissible cancer in both species. Phylogenetic analysis of cancer-associated alleles and analysis of diagnostic SNPs showed that cancers in both species likely arose in a third species of mussel (M. trossulus), but these cancer cells are independent from the previously identified transmissible cancer in M. trossulus from Canada. Unexpectedly, cancers from M. chilensis and M. edulis are nearly identical, showing that the same cancer lineage affects both. Thus, a single transmissible cancer lineage has crossed into two new host species and has been transferred across the Atlantic and Pacific Oceans and between the Northern and Southern hemispheres.

Inter-species difference of copper accumulation in three species of marine mussels: Implication for biomonitoring.

Marine mussels have been used widely as biomonitors of coastal contamination in many countries. Due to the restrain of their geographical distributions, it is often necessary to employ more than one species of mussels within a large-scale biomonitoring program. In the present study, we compared the differences of copper (Cu) bioaccumulation in three species of marine mussels (green mussel Perna viridis, blue mussel Mytilus edulis, and hard-shelled mussel Mytilus coruscus) widely distributing along the Chinese coastal waters, under identical Cu exposure conditions. Over the 21-days exposure to dissolved Cu, the green mussels and blue mussels exhibited comparable newly accumulated Cu concentrations, possibly due to their comparable Cu uptake rate constant ku (blue mussel, 0.573 L g-1 d-1; green mussel, 0.530 L g-1 d-1) and efflux rate constant ke (blue mussel, 0.053 d-1; green mussel, 0.065 d-1). In contrast, there was no net Cu accumulation in the hard-shell mussels, which may be accounted by the lower ku (0.394 L g-1 d-1) but higher ke (0.081 d-1) than the other two mussel species. Further subcellular distribution analyses showed that the cellular debris and metallothionein-like protein (MTLP) fraction were the key binding sites for Cu, and the MTLP fraction may act as a main contributor in Cu regulation and elimination in the blue mussels and hard-shell mussels. There was no strong evidence that the subcellular partitioning and dynamics of Cu in the mussels were responsible for the difference underlying the Cu accumulation in the three species of mussels. Our comparative study thereby suggested that it may be feasible to directly compare the Cu bioavailability in the green mussels and blue mussels based on their Cu biomonitoring data. Cu biomonitoring data from the hard-shell mussels may underestimate the actual Cu bioavailability of the sampling area given its much stronger regulation of Cu bioaccumulation as compared to the other two mussel species.

RNA-seq coupled to proteomic analysis reveals high sperm proteome variation between two closely related marine mussel species

Speciation mechanisms in marine organisms have attracted great interest because of the apparent lack of substantial barriers to genetic exchange in marine ecosystems. Marine mussels of the Mytilus edulis species complex provide a good model to study mechanisms underlying species formation. They hybridise extensively at many localities and both pre- and postzygotic isolating mechanisms may be operating. Mussels have external fertilisation and sperm cells should show specific adaptations for survival and successful fertilisation. Sperm thus represent key targets in investigations of the molecular mechanisms underlying reproductive isolation. We undertook a deep transcriptome sequencing (RNA-seq) of mature male gonads and a 2DE/MS-based proteome analysis of sperm from Mytilus edulis and M. galloprovincialis raised in a common environment. We provide evidence of extensive expression differences between the two mussel species, and general agreement between the transcriptomic and proteomic results in the direction of expression differences between species. Differential expression is marked for mitochondrial genes and for those involved in spermatogenesis, sperm motility, sperm-egg interactions, the acrosome reaction, sperm capacitation, ATP reserves and ROS production. Proteins and their corresponding genes might thus be good targets in further genomic analysis of reproductive barriers between these closely related species. SignificanceModel systems for the study of fertilization include marine invertebrates with external fertilisation, such as abalones, sea urchins and mussels, because of the ease with which large quantities of gametes released into seawater can be collected after induced spawning. Unlike abalones and sea urchins, hybridisation has been reported between mussels of different Mytilus spp., which thus makes them very appealing for the study of reproductive isolation at both pre- and postzygotic levels. There is a lack of empirical proteomic studies on sperm samples comparing different Mytilus species, which could help to advance this study. A comparative analysis of sperm proteomes across different taxa may provide important insights into the fundamental molecular processes and mechanisms involved in reproductive isolation. It might also contribute to a better understanding of sperm function and of the adaptive evolution of sperm proteins in different taxa. There is now growing evidence from genomics studies that multiple protein complexes and many individual proteins might have important functions in sperm biology and the fertilisation process. From an applied perspective, the identification of sperm-specific proteins could also contribute to the improved understanding of fertility problems and as targets for fertility control.

Detection of invasive and cryptic species in marine mussels (Bivalvia, Mytilidae): A chromosomal perspective

Marine mussels illustrate a stunning variability in shape and color. Such variability, added to the scarcity of reliable morphological characters for their identification, can mislead recognition prompting the assignation of specimens of a single species to different ones or incorporate specimens belonging to different taxa into a single one. DNA barcoding is widely used for species identification; however, as this method relies on the previous morphological identification of the specimens, some of the DNA sequences stored in DNA databases are incorrectly assigned to a given species. In view of this uncertainty, further criteria beyond morphological characters and DNA sequences in databases are required to more reliably and accurately identify marine mussels. In this work we mapped ribosomal RNA and histone gene clusters to chromosomes of four species of marine mussels and compared them with those from another eight marine mussel taxa. Specimens of these twelve taxa were also DNA barcoded. Our results clearly demonstrated that the chromosomal analysis of marine mussels could shed light on their identification and, therefore, solve contradictions posed by morphological and molecular data.

Polonium-210 in marine mussels (bivalve molluscs) inhabiting the southern coast of India

The present study focused on the determination of the alpha-emitter, 210Po, in two species of marine mussels (bivalve molluscs) commonly available in the southern coastal region of India. The brown mussel, Perna indica was collected from the west coast and the green mussel, Perna viridis from the east coast. The concentration of 210Po was related to the allometry (length of shell, wet/dry weight of shell/soft tissue) of the mussels and significant results were found. The study period focused on three seasons namely, pre-monsoon, monsoon and post-monsoon for a 1-year period (2010–2011). The results revealed higher activity levels in smaller-sized mussels compared to larger ones. Marked variation in 210Po activity concentration was noted in the whole-body soft tissues between seasons and sampling site (p < 0.05). The dose rate assessment for mussels was performed using the ERICA Assessment tool. The chronic exposure to mussels due to 210Po was found to be lesser than the global benchmark dose rate of 10 μGy h−1. The effective ingestion dose to adults who intake mussels was estimated to be in the range 5.1–34.9 μSv y−1. The measurement contributes to the furthering of knowledge of 210Po, since no data exist in this region.

Physiological energetics and biogeographic range limits of three congeneric mussel species

Closely related species with different physiological tolerances and distributions make ideal systems for documenting range shifts in response to a changing climate. Mytilus edulis, M. trossulus, and M. galloprovincialis are sibling species of marine mussels with distinct biogeographical ranges that are correlated with sea surface temperatures. We determined the scope for growth of these three species at a range of temperatures to determine if energetics could predict their distributions. Scope for growth (SFG) represents energy available for growth and/or reproduction above that necessary for maintenance requirements. The SFG of M. galloprovincialis, the species known to inhabit the warmest habitats, was shifted towards warmer temperatures compared to the other two species, remaining positive until nearly 30 °C. M. edulis, a cold-temperate species, maintained a positive SFG up to 23 °C. M. trossulus, a boreal species, generally was not able to maintain a positive SFG above 17 °C. The warm end of each species' range correlated strongly with the point at which that species' SFG became negative in summer and fall. Energetics at cold temperatures did not predict the cold end of the species' ranges, as there was no clear SFG advantage to explain the dominance of M. trossulus in cold habitats. As sea surface temperatures continue to warm with climate change, the energetics of these three species provide a basis for developing mechanistic models predicting future distribution and productivity changes in mussel populations.

Change and stasis in marine hybrid zones in response to climate warming

AbstractAim We test the prediction that hybrid zones between warm‐ and cold‐adapted species will move towards the territory formerly occupied by the cold‐adapted species in response to a warming climate. We use multiple tests of this prediction to distinguish amongst potential mechanistic hypotheses of responses to climate change.Location We sampled 97 locations on the Atlantic coast of Spain and France and the English Channel that span three hybrid zones formed between two species of marine mussels (Mytilus galloprovincialis and M. edulis).Methods Mussels were sampled in 2005–07 and analysed at a nuclear gene (Glu‐5′) that is diagnostically differentiated between the subject species. Results were compared to those of studies made in the same region over the past two decades. Historical change in sea surface temperature (SST) was analysed using National Oceanic and Atmospheric Administration (NOAA) Optimum Interpolation Daily SST. Species distribution models (random forest and maximum entropy) of the current distribution of mussels were constructed and validated by hindcasting the historical distributions of these species. Validated models were used in combination with forecasts of SST to predict changes in mussel distribution to 2050 and 2100.Results We show that over the past two decades two of the hybrid zones in France have not changed in either position or shape. The third hybrid zone, however, has shifted in the predicted direction, c. 100 km eastward into the warming English Channel. Species distribution modelling strongly implicates changes in winter cold SST as driving this change in the position of one of the hybrid zones. Forecasts of future SST indicate that rapid changes in distribution will occur over the next century.Main conclusions Hybrid zones can be used to conduct repeated tests of ecological responses to climate change and can be valuable in sorting among prospective mechanistic hypotheses that underlie that change. Winter temperatures, but not seasonal high temperature, appear to control the distribution of both species. Species distribution modelling indicates that the collapse of these hybrid zones is imminent, with the rapid expansion of the subtropical species in response to continuing SST warming.

Historical changes in the distributions of invasive and endemic marine invertebrates are contrary to global warming predictions: the effects of decadal climate oscillations

AbstractAim We tested whether a hybrid zone that has formed between an endemic and an invasive species of marine mussel has shifted poleward as expected under a general hypothesis of global warming or has responded instead to decadal climate oscillations.Location We sampled 15 locations on the coast of California, USA, that span the distributions of the two species of marine mussels and their hybrids.Methods Mussels were sampled in 2005–08 and analysed at three nuclear gene loci using methods identical to those used in a study a decade earlier in order to document the genetic architecture of this system. Change in the system was determined by comparing the frequency of species‐specific alleles and multi‐locus genotypes over the intervening decade. Climate variation over the same period was examined by comparing the Pacific Decadal Oscillation (PDO), El Niño/Southern Oscillation (ENSO), upwelling indices and sea surface temperature (SST) during and prior to the study period.Results Contrary to the general expectations of global warming we show that the highly invasive warm‐water mussel Mytilus galloprovincialis and the hybrid zone formed with the endemic species Mytilus trossulus has rapidly contracted southwards. Mytilus galloprovincialis declined in abundance over the northern third of its geographic range (c. 540 km) and has become rare or absent across the northern 200 km of the range it previously colonized during its initial invasion. The distribution of the native species M. trossulus has remained unchanged over the same time period.Main conclusions The large‐scale range shift in the warm‐water invasive species M. galloprovincialis and the hybrid zone it forms with M. trossulus has been exceptionally rapid and is in the opposite direction to that predicted by the global warming hypotheses. This shift, however, is consistent with decadal climate variation associated with the ENSO and the PDO. Since the biogeography of this system was first described in 1999, the PDO has shifted from a warm phase, dominated by frequent and large El Niño events, to a cold‐phase period, with minimal ENSO activity. Thus recent decadal climate variation can oppose global trends in average temperature and this study illustrates the need to integrate the effects of climate change across multiple time‐scales.