Changes In Marine Ecosystems Research Articles

Evaluating zooplankton species diversity using environmental DNA and bulk-DNA metabarcoding in the Ulleung Basin of the Southeastern Korean Peninsula in the summer

Accurately investigating the composition of zooplankton species is crucial for monitoring changes in marine ecosystems and assessing biodiversity. In this study, we utilized bulk DNA and environmental DNA metabarcoding in the Ulleung Basin, known for its high zooplankton species diversity among the seas surrounding the Korean Peninsula. Genomic DNA extracted from samples collected during three summer seasons in the survey area was analyzed using high-throughput sequencing of the cytochrome c oxidase I barcode region. We identified 350 species, which were three to six times more than those identified by traditional morphological methods. Furthermore, we observed significant differences in species composition and diversity between bulk DNA and eDNA samples. Notably, eDNA metabarcoding effectively detected species with high swimming ability and those that were difficult to capture using traditional sampling methods. This study underscores the significant impact of sampling methods on research outcomes in zooplankton species diversity studies and highlights the importance of integrating different sampling techniques. Specifically, it suggests the need for the active adoption of non-invasive methods, such as eDNA metabarcoding, for the comprehensive monitoring of diverse biological groups in marine ecosystems.

A spatial matrix factorization method to characterize ecological assemblages as a mixture of unobserved sources: An application to fish eDNA surveys

Abstract Understanding how ecological assemblages vary in space and time is essential for advancing our knowledge of biodiversity dynamics and ecosystem functioning. Metabarcoding of environmental DNA (eDNA) is an efficient method for documenting biodiversity changes in both marine and terrestrial ecosystems. However, current methods fail to detect and display the biodiversity structure within and between eDNA samples limiting ecological and biogeographical interpretations. We present a spatial matrix factorization method that identifies optimal eDNA sample assemblages—called pools—assuming that taxonomic unit composition is based on a fixed number of unknown sources. These sources, in turn, represent taxonomic units sharing similar habitat properties or characteristics. The method aims to reduce the multi‐taxa composition structure into a low number of dimensions defined by these sources. This method is inspired by admixture analysis in population genetics. Using a marine fish eDNA survey on 263 sampling stations detecting 2888 molecular operational taxonomic units (MOTUs), we apply this method to analyse the biogeography and mixing patterns of fish assemblages at regional and large scales. At large scale, our analysis reveals six primary pools of fish samples characterized by distinct biogeographic patterns, with some mixtures between these pools. We identify pools composed of unique sources, corresponding to distinct and more isolated regions such as the Mediterranean and Scotia Seas. We also identify pools composed of a greater mix of sources, corresponding to geographically connected areas, such as tropical regions. Additionally, we identify the taxa underpinning the formation of each pool. In the regional analysis of Mediterranean eDNA samples, our method successfully identifies different pools, allowing the detection of not only geographic gradients but also human‐induced gradients corresponding to protection levels. Spatial matrix factorization adds a new method in community ecology, where each sample is considered as a mixture of K unobserved sources, to assess the dissimilarity of ecological assemblages revealing environmental and human‐induced gradients. Beyond the study of fish eDNA samples, this method has the potential to shed new light on any biodiversity survey and provide new bioindicators of global change.

Adapting management of Pacific salmon to a warming and more crowded ocean

Abstract The North Pacific Ocean is warming and overall Pacific salmon abundance is higher now than at any other time in the past century. This increase in abundance is in large part due to warming-related changes in marine ecosystems at northern latitudes that primarily benefit pink salmon, and industrial-scale hatchery production to support commercial fisheries. A large body of evidence indicates that increasing and more variable ocean temperatures, as well as competition among salmon at sea, are associated with shifts in salmon productivity, body size, and age at maturation. However, these relationships vary by species, location, and time, resulting in increased harvest opportunities in some regions and exacerbated conservation concerns in others. The weight-of-evidence suggests North Pacific salmon nations should, as a minimum, limit further increases in hatchery salmon production until there is a better scientific understanding of hatchery and wild salmon distribution at sea, how they interact, and how the consequences of these interactions are influenced by broader climate and ecosystem conditions. Coordinated research to overcome knowledge gaps and develop strategies to reduce unintended interactions between hatchery and wild salmon could be funded (in part) by a tax placed on industrial-scale hatchery salmon releases. A tax would formalize recognition that there are finite prey resources to support salmon in the ocean and that both prey and wild salmon represent a “common property” whose use should not be without cost to those that seek to benefit from them. We highlight additional approaches salmon nations can take to adapt to changing conditions and suggest that improved communication and collaboration among North Pacific salmon research and management agencies will be key to balancing the benefits and risks of a warming and more crowded ocean.

Long‐Term Variability of Phytoplankton Primary Production in the Ulleung Basin, East Sea/Japan Sea Using Ocean Color Remote Sensing

AbstractIn recent years, significant changes in environmental conditions and marine ecosystems have been observed in the East Sea/Japan Sea. This study investigates the long‐term environmental dynamics and phytoplankton responses in the Ulleung Basin, situated in the southwestern East Sea/Japan Sea, utilizing satellite and in situ data from 2002 to 2021. Over this period, there was a noticeable increase in sea surface temperature (SST) (r = 0.5739, p < 0.01), accompanied by decreasing mixed layer depth (MLD) and chlorophyll‐a (Chl‐a) concentration (r = −0.6193 and −0.6721, respectively; p < 0.01). Nutrient concentrations within the upper 50 m significantly declined for nitrate and phosphate. A reduction in the N:P ratio indicated a shift from phosphorus‐limited to nitrogen‐limited environment. Moreover, primary production (PP) demonstrated a decreasing trend (r = −0.5840, p < 0.01), coinciding with an increase in small phytoplankton contribution (r = 0.6399, p < 0.01). Rising SST potentially altered the water column's vertical structure, hindering nutrient entrainment from the deep ocean. Consequently, this nutrient limitation may increase small phytoplankton contribution, resulting in a decline in total PP. Under the IPCC's SSP5‐8.5 scenario, small phytoplankton contribution in the Ulleung Basin is projected to rise by over 10%, resulting in a 29% average PP decrease by 2100. This suggests a diminishing energy supply to the food web in a warming ocean, impacting higher trophic levels and major fishery resources. These findings emphasize the critical need for understanding and monitoring these environmental shifts for effective fisheries management and marine ecosystem conservation.

Multiple climatic drivers increase pace and consequences of ecosystem change in the Arctic Coastal Ocean

AbstractThe impacts of climate change on Arctic marine systems are noticeable within the scientific “lifetime” of most researchers and the iconic image of a polar bear struggling to stay on top of a melting ice floe captures many of the dominant themes of Arctic marine ecosystem change. But has our focus on open‐ocean systems and parameters that are more easily modeled and sensed remotely neglected an element that is responding more dramatically and with broader implications for Arctic ecosystems? We argue that a complementary set of changes to the open ocean is occurring along Arctic coasts, amplified by the interaction with changes on land and in the sea. We observe an increased number of ecosystem drivers with larger implications for the ecological and human communities they touch than are quantifiable in the open Arctic Ocean. Substantial knowledge gaps exist that must be filled to support adaptation and sustainability of socioecological systems along Arctic coasts.

A new cockroach (Blattodea, Rhipidoblattinidae) from the Toarcian (Lower Jurassic) of Alderton Hill, Gloucestershire, UK, and the earliest likely occurrence of aposematic colouration in cockroaches

AbstractWe describe the seventh valid species of cockroach, Alderblattina simmsi gen. et sp. nov., from insect‐rich strata recording the Toarcian Oceanic Anoxic Event (T‐OAE). The T‐OAE represents a period of extreme global warming and environmental change that drove palaeoecological pressures and evolutionary changes in marine and terrestrial ecosystems. Here, for the first time, we present evidence that this event may also be the driver for the evolution of aposematic colouration, a predator deterrent, in cockroaches. The specimen, an isolated compressed tegmen, was collected at Alderton Hill, Gloucestershire, UK, and is assigned to a new genus and species, based on the following unique combination of characteristics: small forewing; 15 branches of R and 11 branches of M + CuA; strong cross‐vein between R and M; intercalaries; and two well‐defined subspherical maculae (spots) and colouration at the wing tip. Alderblattina simmsi is assigned to the family Rhipidoblattinidae Rohdendorf, primarily based on its small size and the presence of branched anal veins in the clavus. The colouration present on A. simmsi represents the first recorded likely occurrence of aposematism in cockroaches, and provides evidence for the evolution of colour patterning in Blattodea.

Climate-driven succession in marine microbiome biodiversity and biogeochemical function.

Seasonal and El Niño-Southern Oscillation (ENSO) warming result in similar ocean changes as predicted with climate change. Climate-driven environmental cycles have strong impacts on microbiome diversity, but impacts on microbiome function are poorly understood. We quantified changes in microbial genomic diversity and functioning over 11 years covering seasonal and ENSO cycles at a coastal site in the southern California Current. We observed seasonal oscillations between large genome lineages during cold, nutrient rich conditions in winter and spring versus small genome lineages, including Prochlorococcus and Pelagibacter , in summer and fall. Parallel interannual changes separated communities depending on ENSO condition. Biodiversity shifts translated into clear oscillations in microbiome functional potential. Ocean warming induced an ecosystem with less iron but more macronutrient stress genes, depressed organic carbon degradation potential and biomass, and elevated carbon-to-nutrient biomass ratios. The consistent microbial response observed across time-scales points towards large climate-driven changes in marine ecosystems and biogeochemical cycles.

Microbial hydrogenation of cholesterol to coprostanol by anaerobic bacteria: evidence from Antarctic lacustrine sediment

Fecal sterols are traditionally ascribed as important biomarkers for animal excrement, and have been widely used to identify the source of organic matter and to reconstruct paleoecological changes in Antarctic terrestrial, aquatic, and marine ecosystems. However, the in situ microbial hydrogenation of cholesterol to coprostanol could have significance as a proxy to infer paleoenvironmental studies in Antarctica, particularly in anoxic sediment. Here, we report that abundant coprostanol, which was traditionally deemed as a biomarker for human sewage contamination, was found in three anoxic sediment profiles (AC2, BI, and CH1), which were strongly influenced by animal excrement at North Victoria Land, western Ross Sea, Antarctica. Our results suggest that the high concentrations of coprostanol in these three sediment profiles were not due to animal excrement, since coprostanol is not present in penguin guano and is a minor component in seal excrement. Coprostanol/cholesterol and coprostanol/(coprostanol + cholestanol) ratios suggest that coprostanol in the sediment cores of AC2 and BI was primarily derived from bacterial hydrogenation of cholesterol introduced by penguin guano. Coprostanol in CH1 sediments is related to human sewage due to intensive research activities from 1968 to 2006 in this region. However, the low abundance of coprostanol and the ratios of coprostanol/cholesterol and coprostanol/(coprostanol + cholestanol) in a relatively oxidizing sediment core (IIL1) infer that coprostanol was likely contributed by seal settlement. Together with high-throughput sequencing of the 16S rRNA gene, the conversion of cholesterol to coprostanol by anaerobic bacteria (e.g., Eubacterium coprostanoligenes) could occur in anoxic aquatic systems. Our results suggest that the presence of coprostanol in Antarctic lacustrine sediment with anoxic conditions does not necessarily indicate seal activity and human waste as the microbial hydrogenation of cholesterol to coprostanol should also be considered.

Building collaborative monitoring programs to manage for ecological sustainability in multi-use marine protected areas: Parks Canada’s national marine conservation areas

Parks Canada collaborates with partners to manage a growing system of national marine conservation areas (NMCAs) and national marine conservation area reserves (NMCARs). These ecologically and culturally varied marine protected areas are established to represent the rich diversity of Canada’s oceans and Great Lakes. They support a broad range of uses within specific zones, including recreation, tourism, fishing, shipping, and Indigenous rights-based activities. Long-term monitoring is a critical tool for tracking changes in marine ecosystems, developing strategies to protect and conserve ecological and cultural values, responding to environmental threats, and ensuring ecologically sustainable use of marine resources that provide long-term societal benefits. Monitoring involves working closely with Indigenous partners, federal departments, provincial/territorial governments, coastal communities, and others (e.g., fishing and tourism sectors, non-governmental organizations, academia). As part of larger seascapes, NMCAs are managed using an ecosystem approach, which requires information on the state of marine biodiversity, threats, and pressures on marine ecosystems, including levels or impacts of marine uses. Each NMCA monitors a site-specific suite of indicators covering these key characteristics that are then integrated to inform decision-making. The monitoring framework is intended to enable a comprehensive, nationally consistent approach to monitoring and reporting while adapting to local needs and governance. Successes, challenges, and lessons learned through the implementation of the framework are highlighted through place-based examples.

First Record of the Red Cornetfish Fistularia petimba Lacepède, 1803 from Amorgos Island (Central Aegean Sea; Greece) and a Review of Its Current Distribution in the Mediterranean Sea

The rapid spread of non-native species (NNS) poses a significant threat to biodiversity globally, with the Mediterranean region being particularly susceptible due to increased human activities and its status as a marine biodiversity hotspot. In this study, we focus on the introduction and distribution of Fistularia petimba, a member of the Fistulariidae family, in the eastern Mediterranean Sea and a record from the coasts of Amorgos Island, Greece. Through a baseline fishery study conducted over 12 months, utilizing experimental sampling with gillnets, trammel nets, and longlines, one individual of F. petimba was captured off the coast of Katapola Bay. Morphological examination confirmed its identity, with measurements on meristic characteristics obtained and the stomach content analysed. This finding represents a significant addition to the documented distribution of F. petimba in the Mediterranean Sea, particularly in the Aegean Sea, underscoring the importance of ongoing research in uncovering new occurrences and expanding our understanding of marine biodiversity and ecosystem changes. Further investigation into the ecological preferences and population dynamics of F. petimba in the Aegean Sea is crucial for informed conservation and management efforts if this species is considered to be established.

Detection of Cyclic Imines (CIs) Toxins in Whole Body of Shellfishes: First Monitoring Report of CIs in South Korea Shellfishes

Cyclic imines (CIs) produced by microalgal species that accumulate in the food chains of marine organisms are novel biotoxins that do not belong to the classic group of marine biotoxins. In the past, CIs were found only in limited areas; however, in recent years, rapid changes in marine ecosystems have led to widespread CIs and increased exposure to toxic risks. In this study, we analyzed seven CI toxins, GYM-A, SPX (13-desmethyl spirolide C, 13, 19-dideMe spirolide C, 20-methyl spirolide G), and PnTX-E, F, and G, using LC/MRM-MS. Shellfish samples were purchased from a domestic Korean fish market (67 samples in 2021 and 216 samples in 2022). The entire body of the shellfish was ground and extracted with 50% methanol, followed by lipophilic-specific SPE. Only GYM-A, PnTX-G, and 13-desmethyl spirolide C were detected in all analyzed samples. The maximum concentrations of GYM-A is maximum 179 ppt (ng/kg) in Crassostrea nippona (March 2022), PnTX-G is maximum 7 ppt in Anadara broughtonii (April 2022), 13-desmethyl SPX C is maximum 58 ppt in Crassostrea nippona (April 2022). The southern coast exhibited the highest frequency of detection of these toxins, which was attributed to elevated sea-surface temperatures, aligned with conducive conditions for toxin-producing phytoplankton. According to the monitoring results, there were no significant CI toxins in the shellfish; however, it is important to monitor CI toxin accumulation in shellfish because of their high risk of toxicity.

Intact stalked crinoids from the late Aptian of NE Spain offer insights into the Mesozoic Marine Revolution in the Tethys

Abstract Stalked crinoids were common in shallow-marine habitats in the geologic past but are today restricted to the deep sea. The timing of the shift in their bathymetric distribution has been discussed in the context of the Mesozoic Marine Revolution (MMR), one of the most important episodes of change in marine ecosystems of the Phanerozoic, which is generally thought to have begun sometime in the Late Triassic. However, the record of Cretaceous stalked crinoids is poor, consisting primarily of disarticulated ossicles for which the provenance is often difficult to determine, hampering interpretations of the habitat in which they lived. Here, we report on well-preserved isocrinids from the Aptian and use a multidisciplinary approach involving sedimentological, isotopic, and paleontological data to demonstrate that they inhabited a shallow lagoon subject to salinity variations. This suggests that their absence from such environments today is not a function of physicochemical factors and reinforces the idea that predation pressure may have played a critical role in their bathymetric distribution. The influence of predation is supported by the first Early Cretaceous record of a regenerating arm.

On the Sea Surface Temperature Forecasting Problem with Deep Dilation-Erosion-Linear Models

The sea surface temperature (SST) is considered an important measure for detecting changes in climate and marine ecosystems. So, its forecasting is essential for supporting governmental strategies to avoid side effects on the global population. In this paper, we analyze the SST time series and suggest that a combination between a linear component and a nonlinear component with long-term dependency can better represent it. Based on this assumption, we propose a deep neural network architecture with dilation-erosion-linear (DEL) processing units to deal with this particular kind of time series. An empirical analysis is performed in this work using three SST time series, where we explore three statistical measures. The experimental results demonstrate that the proposed model outperformed recent and classical literature forecasting techniques according to well-known performance metrics.

Meiobenthos and ocean acidification: Effects on meiobenthic communities inhabiting Mediterranean cold shallow CO2-vents

Ocean acidification is causing major changes in marine ecosystems, with varying levels of impact, depending both on the habitat and the studied organisms. Here, we investigated for the first time the meiobenthos and nematode fauna inhabiting the sediments around Castello Aragonese (Ischia, Italy), characterized by variable pH values due to coastal volcanic CO2 venting. In this scenario, nematode functional diversity changed according to different pH levels and grain size: maturity index was higher at most acidic stations and trophic composition spanned from the dominance of predators in the acidic stations, to the high abundance of non-selective deposit feeders and epistrate feeders in the ambient-pH stations. Overall, the present study revealed a relatively high tolerance of meiobenthos and nematodes to lower pH conditions. However, an in-depth analysis of nematode fauna showed differences in their assemblages at different pH levels with few nematode genera rather adapted to the extreme environmental conditions at the acidic stations.

Zooarchaeological perspectives in the framework of the Anthropocene: Contributions to ecological, environmental and conservation studies from South America

This special volume considers major recent changes in southern South American animal communities. Eleven papers consider megafauna, pinnipeds, marine mammals, small terrestrial mammals and birds and are grouped under four sub-headings: (1) Isotopic insights into guanaco populations; (2) Historical sources and marine ecosystem change; (3) Changes in small mammal communities and human impacts; and (4) megafaunal extinction, domestication, avifauna and recent interactions with humans. Although some of these contributions include changes that occurred earlier in the Holocene, many highlight a current decrease in the taxonomic diversity of communities and ecosystems in different environments, which are likely to have been caused by modern human activities. The Anthropocene concept is seen as providing a useful framework for understanding and mitigation of such adverse human impacts.

Fatty acid production and associated gene pathways are altered by increased salinity and dimethyl sulfoxide treatments during cryopreservation of Symbiodinium pilosum (Symbiodiniaceae)

The Symbiodinium genus is ancestral among other Symbiodiniaceae lineages with species that are both symbiotic and free living. Changes in marine ecosystems threaten their existence and crucial ecological roles. Cryopreservation offers an avenue for their long-term storage for future habitat restoration after coral bleaching. In our previous study we demonstrated that high salinity treatments of Symbiodiniaceae isolates led to changes in their fatty acid (FA) profiles and higher cell viabilities after cryopreservation. In this study, we investigated the role of increased salinity on FA production and the genes involved in FA biosynthesis and degradation pathways during the cryopreservation of Symbiodinium pilosum. Overall, there was a twofold increase in mass of FAs produced by S. pilosum after being cultured in medium with increased salinity (54 parts per thousand; ppt). Dimethyl sulfoxide (Me2SO) led to a ninefold increase of FAs in standard salinity (SS) treatment, compared to a fivefold increase in increased salinity (IS) treatments. The mass of the FA classes returned to baseline during recovery. Transcriptomic analyses showed an acyl carrier protein gene was significantly upregulated after Me2SO treatment in the SS cultures. Cytochrome P450 reductase genes were significantly down regulated after Me2SO addition in SS treatment preventing FA degradation. These changes in the expression of FA biosynthesis and degradation genes contributed to more FAs in SS treated isolates. Understanding how increased salinity changes FA production and the roles of specific genes in regulating FA pathways will help improve current freezing protocols for Symbiodiniaceae and other marine microalgae.

Meiofauna community structure in the Yellow Sea Cold Water Mass and non‐Yellow Sea Cold Water Mass

This study investigated meiofaunal community structure over a large area of the Yellow Sea and East China Sea for 45 stations from 2021 to 2022. Meiofaunal densities were 34 ± 15–1610 ± 378 ind./10 cm2. Nematodes (67.3%) were the most dominant taxa, followed by benthic foraminifera (12.0%) and benthic harpacticoids (8.8%). The study was conducted in various sedimentary environments. Median grain size, water depth, and environmental characteristics (TOC, TN, Hg, Sr) were identified as influencing meiofauna community structure. The Yellow Sea and East China Sea, where this study was conducted, contain a variety of currents and the Yellow Sea Cold Water Mass (YSCWM) in the central waters. YSCWM and non-YSCWM areas differed in their meiofauna community structure. Even within the YSCWM region, we found differences in benthic community structure due to a variety of environmental factors. The meiofaunal taxon most affected by Sediment temperature was benthic foraminifera. Identifying foraminifera in paleo-oceanographic environments can indicate past expansion and contraction of YSCWM. Meiofauna have been used to monitor changes in marine ecosystems due to their sensitivity to changes in the surrounding environment. Meiofauna index (N/C ratio, MPI) were identified as appropriate for use in the study area.

Can the delayed effects of climatic oscillations have a greater influence on global fisheries compared to their immediate effects?

Climatic oscillations affect fish population dynamics, ecological processes, and fishing operations in maritime habitats. This study examined how climatic oscillations affect catch rates for striped, blue, and silver marlins in the Atlantic Ocean. These oscillations are regarded as the primary factor influencing the abundance and accessibility of specific resources utilized by fishers. Logbook data were obtained from Taiwanese large-scale fishing vessels for climatic oscillations during the period 2005-2016. The results indicated that the effect of the Subtropical Indian Ocean Dipole on marlin catch rates did not have a lag, whereas those of the North Atlantic Oscillation, Atlantic Multidecadal Oscillation, Pacific Decadal Oscillation, and Indian Ocean Dipole had various lags. Pearson's correlation analysis was conducted to examine the correlations between atmospheric oscillation indices and marlin catch rates, and wavelet analysis was employed to describe the influences of the most relevant lags. The results indicated that annual atmospheric fluctuations and their lags affected the abundance and catchability of striped, blue, and silver marlins in the study region. This, in turn, may affect the presence of these species in the market and lead to fluctuations in their prices in accordance with supply and demand. Overall, understanding the effects of climatic oscillations on fish species are essential for policymakers and coastal communities seeking to manage marine resources, predict changes in marine ecosystems, and establish appropriate methods for controlling the effects of climate variability.

Microfluidics for macrofluidics: addressing marine-ecosystem challenges in an era of climate change.

Climate change presents a mounting challenge with profound impacts on ocean and marine ecosystems, leading to significant environmental, health, and economic consequences. Microfluidic technologies, with their unique capabilities, play a crucial role in understanding and addressing the marine aspects of the climate crisis. These technologies leverage quantitative, precise, and miniaturized formats that enhance the capabilities of sensing, imaging, and molecular tools. Such advancements are critical for monitoring marine systems under the stress of climate change and elucidating their response mechanisms. This review explores microfluidic technologies employed both in laboratory settings for testing and in the field for monitoring purposes. We delve into the application of miniaturized tools in evaluating ocean-based solutions to climate change, thus offering fresh perspectives from the solution-oriented end of the spectrum. We further aim to synthesize recent developments in technology around critical questions concerning the ocean environment and marine ecosystems, while discussing the potential for future innovations in microfluidic technology. The purpose of this review is to enhance understanding of current capabilities and assist researchers interested in mitigating the effects of climate change to identify new avenues for tackling the pressing issues posed by climate change in marine ecosystems.

Prvi popis hrskavičnjača Crne Gore temeljen na kombinaciji monitoringa gospodarskog ribolova i građanske znanosti

Chondrichthyans are considered a highly threatened marine species, due both to the intense fishing pressure they have experienced in the world’s Oceans over several decades, and other threats, such as habitat loss, climate change and marine pollution. A rapid decline in their populations could trigger significant negative changes in marine ecosystems, highlighting the need for improved conservation measures. This study analyzes the current state of this group in the waters of Montenegro (in the southeastern Adriatic Sea). The first national checklist is provided here, comprising of 44 species, with information on their occurrence frequencies in Montenegrin fishery catches. The dataset used consists of 1469 records obtained from 281 field samplings conducted as part of the official monitoring of commercial fisheries (DCF-DCRF) aswell as 1222 records derived from citizen science. The latter contributed significantly in documenting a greater number of species, particularly those considered threatened or rare in the region, and/or overlooked by DCF-DCRF monitoring. The frequency of species in Montenegrin catches is described based on the percentage of their records in the total number of records originating from local fisheries. The obtained results indicate that only 16% of the species recorded in Montenegro are commonly present in country’s catches, while 66% are either rare or not observed in local fishery. The creation of the country’s first checklist might contribute to overcoming drawbacks in national legislation and protection measures, notably by reporting the presence of endangered species in national marine waters.