Marginal Ecosystems Research Articles

A little less structuration, a little more action: Examining entrepreneurial agency in two marginal ecosystems

Entrepreneurs form and grow businesses in relation to the localised context around them (structure) and also through their own choices and actions (agency). In this way, structure and agency intersect in the lived experiences of entrepreneurs. The purpose of this work is to explore entrepreneurial agency in two ecosystems that, as structures, constrain entrepreneurship within them: Newcastle Upon Tyne (UK) and Perth (Australia). We add to extant analysis that empirically looks at the active agency entrepreneurs participate in, as they attempt to overcome the structural level constraints they encounter. Methodologically, we draw on phenomenological data, arriving at a highly detailed insight, drawing attention to the strong local entrepreneurial communities and distinctive cognitive mindsets that entrepreneurs in the ecosystems we study have agentically developed and cultivated. We suggest how future analysis of entrepreneurial agency may unfold and consider the implications of our insight from a policy implementation perspective.

An assessment of soil microbial community environmental stress in alpine marginal ecosystems using microbial indicators

Alpine marginal ecosystems above the natural tree line are considered to be the most vulnerable habitats for maintaining local biodiversity. The soil microbial community is crucial for ecosystem stability and function. However, our comprehension of the overall soil microbial community in alpine marginal ecosystems remains limited, particularly regarding environmental stress. We selected the Baima Mountain National Nature Reserve (BMNNR) in the northwest Yunnan provinces as our study area and collected soil samples from three different depth layers, in alpine marginal ecosystems. The phospholipid fatty acid (PLFA) analysis method was utilized to evaluate alterations in soil microbial communities. The results showed that: (1) the PLFA content gradually decreased with increasing soil depth; (2) on the whole, the variations in PLFA across alpine marginal ecosystems were obviously, the highest in alpine meadow (AM) and the lowest in alpine screes (AS); (3) the site effect exerted a greater direct influence than microhabitat effect and elevation (0–5 cm, 32 %, 16 % and 9 %, respectively; 20–40 cm, 46 %, 29 % and 0 %, respectively), with the direct impact of elevation decreasing as soil depth increased (0–5 cm, 5–20 cm and20-40 cm, 9 %, 1 % and 0 %, respectively); and (4) the ratio of cyclopropyl to precursors (Cy:Pre) in all communities was > 0.1, indicating that the soil microbial community was under environmental stress. This study demonstrates the potential of PLFA analysis to profile soil microbial communities and their stress status under field conditions and also provides a better understanding of the correlation between soil microbial communities and the function and health of alpine marginal ecosystems.

Middle Holocene Lithic Technological Organization and Upland Land Use in the Upper Susitna River Basin, Alaska

ABSTRACT The middle Holocene period is marked by an apparent increase in the use of upland subsistence resources across North America. This pattern has also been observed in the uplands of central Alaska; however, we have limited understanding of the nature of upland hunter-gatherer land-use strategies during this time. This study presents a lithic technological organization analysis of three middle Holocene sites in the upper Susitna River basin, central Alaska Range, to evaluate middle Holocene land-use strategies. The Middle Holocene lithic assemblages presented here have characteristics of a low mobility land-use system in contrast with preceding periods. An important driver of middle Holocene upland land-use intensification may have been a warming climate and associated shift in vegetation patterns and caribou range. Understanding the intensity with which hunter-gatherers in the past utilized upland landscapes has implications for understanding ecological change in marginal ecosystems.

Effect of nitrogen and glyphosate on the plant community composition in a simulated field margin ecosystem: Model-based ordination of pin-point cover data

The effect of nitrogen and glyphosate on the plant community composition was investigated in a simulated field margin ecosystem. The plant community composition was inferred from pin-point cover data using a model-based ordination method that is suited for modelling pin-point cover data. The mean structure of the ordination model is analogous to a standard linear model, which enabled us to estimate the mean effects of nitrogen and glyphosate and their interaction in the two-dimensional ordination space. There were significant effects of both nitrogen and glyphosate on the plant community composition and overall species diversity. The effects of nitrogen and glyphosate on the plant community composition differed significantly. Furthermore, the estimated combined effects of nitrogen and glyphosate indicated that nitrogen and glyphosate enforced the effect of each other on the plant community composition by synergistic interactions. Addition of nitrogen and glyphosate was found to favor a plant community that was dominated by perennial grasses, and there was a tendency for glyphosate to select for plant communities in which annual plants were more frequent. The results suggest that using the notion of plant functional types and specific knowledge of the degree of glyphosate tolerance may be effective for predicting the effect of glyphosate on the community composition.

The Nutritional, Medicinal, and Drought-Resistance Properties of Ziziphus Mill. Make It an Important Candidate for Alleviating Food Insecurity in arid Regions—A Case of Pakistan

In this article, we elaborate on why the cultivation of the Ziziphus is important in the arid regions of Pakistan and how it can alleviate the poverty and food insecurity in these areas. The recent impact of climate change has resulted in the spread of hot and dry regions. The cultivation of Ziziphus species could be a solution to food insecurity and income for the residents of arid and semi-arid regions in Pakistan. Furthermore, Ziziphus species are offering extremely nutrient-dense foods. Six species of Ziziphus are found in Pakistan, but production statistics are not available for all species. The most economically important species are Z. jujuba and Z. mauritiana, followed by Z. nummularia, based on the consumption of their fruits. A framework of policy and practical proposals required for the cultivation of these species in Pakistan has been provided. Ziziphus, an economically important genus of fruit-bearing plant species, is cultivated in arid and semi-arid regions of the world. Therefore, the species of this genus could be efficiently and successfully cultivated in marginal ecosystems for alleviating food insecurity and poverty reduction in Pakistan. Furthermore, Ziziphus species like Z. jujuba and Z. mauritiana have promise as a cash and food crop, especially in water-deficient areas of Pakistan, and their production and conservation must be encouraged by state policy.

Ultra-conserved elements provide insights to the biogeographic patterns of three benthic macroinvertebrate species in the Baltic Sea

The Baltic Sea, with its steep salinity gradient, high water retention time, and relatively young age, represents a marginal ecosystem between marine and freshwater extremes. Due to differing invasion history and dispersal capabilities of Baltic species, there are large differences in species distributions, species-specific genetic structure and variation, and edge populations that may represent both a subset of the original population, as well as unique genetic lineages. We used a phylogenomic approach to investigate relationships between populations of three benthic macroinvertebrate species: Pygospio elegans, Corophium volutator, and Mya arenaria, providing new insight into evolutionary dynamics among populations in the Baltic Sea and the adjacent North Sea. We found little relation among the populations of P. elegans and C. volutator, in contrast to M. arenaria, which exhibits a higher degree of resemblance between populations. We also found low relation within sites sampled at different times of the year for all species. Each species exhibited unique phylogenetic patterns, suggesting the North Sea populations of P. elegans and M. arenaria are closely related to populations within the Baltic Sea, and with only C. volutator showing trends resembling isolation by distance. These differences could be explained by both their different invasion histories and dispersal capabilities of the individual species.

Swot Analysis of Peatland Utilization Assessment for Community (Case Study at Banjar Regency of South Kalimantan)

<p>Peatland is a unique, marginal, and fragile ecosystem. Its utilization must be based on thorough research and planning, and it needs supports from everyone. Yards, fields, and gardens are the forms that the community generally uses to utilize peatlands. This research aims to assess community's activities in the peatlands as their source of living and income. This research was conducted in Indonesia, specifically in the Province of South Kalimantan within an area with the biggest peatland contour. Descriptive data research was analyzed using thematic, socio-economic, and SWOT analysis. Research finding showed that farming is the suitable source of livelihood in the peatlands, besides plantation and other fields. The highest income from non-land activities on the peatlands are from entrepreneurship, physical service, and animal husbandry. People's general livelihood are as farmers whose prior commodity is with bigger average income than the province minimum wage per month. This is expected to describe that income from peatlands can make the ends meet of the community. This can also increase farmer's knowledge in cultivating their lands, the choice of plant type that is suitable with biophysical conditions of the peatlands. To work on the limit of the community's knowledge, activities and solutions from the experts on how to deal with farmer's problems in cultivating peatlands, are needed. Agroforestry technique is very suitable to analyze the source of livelihood. As forms to reserve lands in a farming world, the use of husk charcoal is also suggested. It has abundant benefits. It is often used for soil loosening, even for composting. Farmers make use of it for making compost, bokashi, Takakura, planting media, and seedbed media.</p>

Сохранение и развитие морских и прибрежных экосистем: полярный фактор

The paper presents an analysis of polar ecological policy actors. From this perspective, authors discuss in detail a role of state in conservation and development of marine and coastal ecosystems. Furthermore, they describe a green agenda for polar regions. At last, some ways for creating of ecological policies in the Arctic and the Antarctic regions, most especially on behalf of sea and marginal ecosystems conservation and evolution, are proposed. One of the most vital parts of such policies is staff training. The authors have attempted a comprehensive analysis of the Green Agenda at the global, national and local levels in terms of the effectiveness of its proposed mechanisms for the protection of biodiversity in polar areas. Researchers have also reviewed plans of Russia according to the new set of strategies for Arctic and Antarctic regions, which were adopted in 2020. The ideas proposed by the authors can be used in a real-case scenario both for strategies implementation and for public discussion on the global ecological problems.

Untangling the molecular basis of coral response to sedimentation.

Urbanized coral reefs are often chronically affected by sedimentation and reduced light levels, yet many species of corals appear to be able to thrive under these highly disturbed conditions. Recently, these marginal ecosystems have gained attention as potential climate change refugia due to the shading effect of suspended sediment, as well as potential reservoirs for stress-tolerant species. However, little research exists on the impact of sedimentation on coral physiology, particularly at the molecular level. Here, we investigated the transcriptomic response to sediment stress in corals of the family Merulinidae from a chronically turbid reef (one genet each of Goniastrea pectinata and Mycedium elephantotus from Singapore) and a clear-water reef (multiple genets of G. pectinata from the Gulf of Aqaba/Eilat). In two ex-situ experiments, we exposed corals to either natural sediment or artificial sediment enriched with organic matter and used whole-transcriptome sequencing (RNA sequencing) to quantify gene expression. Analysis revealed a shared basis for the coral transcriptomic response to sediment stress, which involves the expression of genes broadly related to energy metabolism and immune response. In particular, sediment exposure induced upregulation of anaerobic glycolysis and glyoxylate bypass enzymes, as well as genes involved in hydrogen sulphide metabolism and in pathogen pattern recognition. Our results point towards hypoxia as a probable driver of this transcriptomic response, providing a molecular basis to previous work that identified hypoxia as a primary cause of tissue necrosis in sediment-stressed corals. Potential metabolic and immunity trade-offs of corals living under chronic sedimentation should be considered in future studies on the ecology and conservation of turbid reefs.

Enriched CO2 and Root-Associated Fungi (Mycorrhizae) Yield Inverse Effects on Plant Mass and Root Morphology in Six Asclepias Species.

While milkweeds (Asclepias spp.) are important for sustaining biodiversity in marginal ecosystems, CO2 flux may afflict Asclepias species and cause detriment to native communities. Negative CO2-induced effects may be mitigated through mycorrhizal associations. In this study, we sought to determine how mycorrhizae interacts with CO2 to influence Asclepias biomass and root morphology. A broad range of Asclepias species (n = 6) were chosen for this study, including four tap-root species (A. sullivantii, A. syriaca, A. tuberosa, and A. viridis) and two fibrous root species (A. incarnata and A. verticillata). Collectively, the six Asclepias species were manipulated under a 2 × 2 full-factorial design that featured two mycorrhizal levels (−/+ mycorrhizae) and two CO2 levels (ambient and enriched (i.e., 3.5× ambient)). After a duration of 10 months, Asclepias responses were assessed as whole dry weight (i.e., biomass) and relative transportive root. Relative transportive root is the percent difference in the diameter of highest order root (transportive root) versus that of first-order absorptive roots. Results revealed an asymmetrical response, as mycorrhizae increased Asclepias biomass by ~12-fold, while enriched CO2 decreased biomass by about 25%. CO2 did not impact relative transportive roots, but mycorrhizae increased root organ’s response by more than 20%. Interactions with CO2 and mycorrhizae were observed for both biomass and root morphology (i.e., relative transportive root). A gene associated with CO2 fixation (rbcL) revealed that the two fibrous root species formed a phylogenetic clade that was distant from the four tap-root species. The effect of mycorrhizae was most profound in tap-root systems, as mycorrhizae modified the highest order root into tuber-like structures. A strong positive correlation was observed with biomass and relative transportive root. This study elucidates the interplay with roots, mycorrhizae, and CO2, while providing a potential pathway for mycorrhizae to ameliorate CO2 induced effects.

Spatial phylogenetics of two topographic extremes of the Hengduan Mountains in southwestern China and its implications for biodiversity conservation

Previous attempts to elucidate the drivers of speciation mechanisms and spatial distribution patterns of biodiversity in mountain regions have treated different floras within a single geological region as one flora, ignoring the potential contributions of high habitat/ecosystem heterogeneity. Furthermore, current conservation strategies largely focus on forest ecosystems and/or specific flagship species, ignoring marginal ecosystems, leaving species in these ecosystems at risk. Here, we compared the spatial patterns of biodiversity and the potential drivers of these patterns in the river valley and subnival ecosystems of the Hengduan Mountains region (HDM) in southwestern China. Specifically, we compared spatial patterns of diversity, endemism, and threatened species in these ecosystems based on both traditional measurements and recent phylogenetic approaches. We then examined how those patterns were related to environmental factors and human activity in these same regions. We found that the middle-southern HDM supports the highest diversity and endemism for the river valley and subnival ecosystems; however, the distribution patterns of neo- and paleo-endemism in these two ecosystems differ. Regression models indicate that habitat diversity and paleo-climatic fluctuation are important drivers of diversity and endemism for these two ecosystems. Temperature and precipitation, however, showed different influences on the spatial patterns in different ecosystems. Categorical analysis of neo- and paleo-endemism (CANAPE) indicated that most endemism centers are not covered by current nature reserves. Moreover, the intensity of human activity is highest in the southern and southeastern HDM, which coincides with the distribution patterns of diversity, mixed-endemism and high-priority (and threatened) species. These findings suggest that different floras within a single geographic/floristic region respond differently to environmental factors and show different spatial phylogenetic patterns. We, therefore, recommend that future research into the drivers of biodiversity consider the contributions of various ecosystem types within a single geological region. This study also provides a theoretical basis for protecting habitat diversity. Our work confirms that current conservation efforts are insufficient to protect ecosystem diversity in the river valley and subnival ecosystems of the Hengduan Mountains. Therefore, we recommend the establishment of nature reserves in the regions identified in this study; furthermore, we strongly recommend improving current and establishing new management policies for biodiversity conservation in this region.

Intra-varietal stability performance of popular rice landrace ‘C14-8’ in the Andaman Islands

The multitude of agronomic merits of a traditionally tall and photosensitive japonica rice landrace C14-8 has enabled its popularity in the tropical Andaman and Nicobar Islands, India. However, we noticed distinct variation for grain husk colour in this culture. Field evaluation of four grain husk color selections over 4 years across three major rice growing islands revealed significant variation for agro-morphological traits studied. In the overall population, harvest index was identified as the potent selectable trait for indirect selection. Through AMMI stability analysis, the environmental, G × E interaction and genotype effects were recorded as 24.4%, 12.5% and 12.3%, respectively. The highest positive genotypic index was recorded at C14-8-11-108 (0.39) followed by C14-8-11-113 (0.29) and C14-8-11-91 (0.20) which also out-yielded the original population by about 20% across years thus indicating the consistency and favorability of these selections under marginal ecosystem. The findings of this paper will be useful for the breeding and conservation perspectives of such unique germplasm having climatically adaptive traits under marginal ecosystems.

Effect of adoption of sustainable crop production systems on farm economics

This study uses farm models to analyze the effect of farm size on farm-level costs of adoption of reduced soil cultivation systems. In an empirical study in the Kulunda Steppe in Russia, we examine the farm-level economics of three crop cultivation technologies: old Soviet intensive tillage technology (OS), modified Soviet reduced-tillage technology (MS), and modern Canadian no-till technology (MC). We consider economies of size by considering three farm sizes (small = 500 ha, medium = 5000 ha, and large = 15,000 ha). Based on the general approach to cost and activity accounting, we sequentially compute gross cost–benefit measurement to assess the economic performance of the different systems. The study utilizes two data sources: (1) experts’ estimates regarding the input requirements and yield impacts of reduced-tillage systems in the marginal ecosystem of the Kulunda Steppe and (2) market prices in 2014 and 2015. According to the model calculations, OS is inferior to MC and MS tillage systems in all farm sizes if we adopt a medium- to long-term perspective and consider residual income. If we adopt a very short-term perspective and consider only the difference between sales and direct costs, the MC tillage system would rank first across all modeled farm sizes. However, because farms in the Kulunda Steppe are heterogeneous, our results cannot replace management decisions based on farm-specific calculations that consider the conditions of the respective farm.

High diversity of benthic bacterial and archaeal assemblages in deep-Mediterranean canyons and adjacent slopes

Submarine canyons and slopes increase the topographic heterogeneity of continental margins and enhance mass and energy transfer from the shelf to the deeper basins, profoundly influencing the biodiversity and functioning of deep-sea ecosystems. However, information on the distribution and diversity of microbial assemblages in such habitats is extremely scant. In the present study, for the first time, we investigated abundances of benthic prokaryotes and their diversity (i.e., bacterial and archaeal) in two of the largest canyons of the Mediterranean Sea (Bisagno and Polcevera Canyons, in the Ligurian Margin) and their adjacent open slope along a water-depth gradient from 200 to 2000 m. We found that prokaryotic abundance and the richness of Operational Taxonomic Units (OTUs) varied within a narrow range despite the high variability of thermohaline and trophic conditions in the habitats investigated. Conversely, the taxonomic composition (in terms of OTUs) was highly variable among the different habitats and depths investigated. The largest fraction of OTUs (representing >80% of the total OTUs identified) were unique of each sampling site, indicating a high prokaryotic β-diversity among the investigated systems. Thermo-haline and trophic conditions and water depth only partially influenced the composition of the prokaryotic assemblages suggesting that other factors, potentially related to physical forcing typically occurring in margin ecosystems, could promote a high diversification of benthic bacterial and archaeal assemblages. This study provides new insights into the benthic prokaryotic diversity of Ligurian canyons and suggests that microbial assemblages thriving in continental margins can largely contribute to the whole diversity of Mediterranean deep-sea ecosystems.

Distribution Modeling ofAsclepiasspp. Predicts Potential Conservation Benefits of Marginal Ecosystems as Nature Reserves in Northwest Ohio

Due to grassland and prairie conversion for agriculture, milkweed species (Asclepias spp.) are now confined to small natural areas, agricultural fields, and marginal ecosystems (e.g., ditches and field margins). Monarch butterflies (Danaus plexippus) rely exclusively on milkweed and are in decline in part due to declining milkweed populations. We conducted a species distribution modeling exercise using MaxEnt to model milkweed species and common milkweed (Asclepias syriaca) presence in natural areas and ditches throughout northwest Ohio in order to identify which local or regional landscape attributes most strongly influence milkweed presence. We found that while natural areas and ditches have similar presence probability ranges (milkweed: natural areas 3–98% and ditches 1–98%; common milkweed: natural areas and ditches 0–98%), natural areas have a higher average probability of presence than ditches (milkweed: natural areas = 30 ± 26% and ditches = 7 ± 7%; common milkweed: natural areas = 22 ± 26% and ditches = 5 ± 6%). However, due to the large area covered by ditches and field margins in our study system, ditches and field margins with a probability of milkweed occurrence greater than the average probability of occurrence in natural areas encompass a larger total area than natural areas. We also observed that the presence of milkweed is positively correlated with the presence of shrub and C4 grasses. We conclude that marginal ecosystems in ditches and field margins are an important component of regional milkweed and, thusly, monarch conservation by providing potential habitat across a diffuse network.

Water Mass Control on Phytoplankton Spatiotemporal Variations in the Northeastern East China Sea and the Western Tsushima Strait Revealed by Lipid Biomarkers

AbstractContinental margin ecosystems in the western North Pacific Ocean are subject to strong climate forcing and anthropogenic impacts. To evaluate mechanisms controlling phytoplankton biomass and community structure variations in marginal sea‐open ocean boundary regions, brassicasterol, dinosterol, and C37 alkenones were measured in suspended particles in summer and autumn from 2012 to 2013 in the northeastern East China Sea and the western Tsushima Strait. In summer, the concentrations of brassicasterol (40–1535 ng/L) and dinosterol (4.2–94 ng/L) were higher in the southwest of Cheju Island, while C37 alkenones (0–30 ng/L) were higher in the south of Cheju Island. In autumn, brassicasterol (12–106 ng/L), dinosterol (2.4–21 ng/L), and C37 alkenones (0.7–7.0 ng/L) were higher in the southwest of Cheju Island and the western Tsushima Strait, and higher C37 alkenones also occurred in the Okinawa Trough. Correlation analysis of biomarkers and environmental conditions (temperature, salinity, and inorganic nutrient concentrations) clearly demonstrated that phytoplankton biomass and community structure variations can be well elucidated by water masses as indexed by temperature and salinity. High nutrients from the Changjiang River were the main cause of high biomass in summer, while nutrients from subsurface water were likely the key factor regulating phytoplankton biomass in open ocean water stations in autumn. This study indicates that mechanisms controlling phytoplankton biomass in marginal sea‐open ocean boundary regions should be classified by various water masses with different nutrient concentrations, instead of by geography.

The cost effectiveness of payments for ecosystem services—Smallholders and agroforestry in Africa

Certain smallholder farmers in parts of sub-Saharan Africa engage in conservation agriculture and participate in agroforestry ecosystem services schemes that generate additional on-farm revenues through payment for ecosystem services (PES). However, smallholder farmers that are inadequately compensated for the foregone income from agricultural production due to marginal ecosystem services provision have no incentive to participate. On the other hand, over-compensating participating farmers will also lead to PES schemes inefficiencies. Therefore, stakeholders are confronted with the challenge of evaluating farm-level interactions between agricultural production and ecosystem services’ provision when making strategic decisions on the efficient level of compensation. We propose in this contribution to assess the efficiency of PES schemes by measuring the marginal cost of ecosystem services based on farm level bio-economic interactions. The quantitative assessment is based on a theoretical classification of the relationship between marketed agricultural output and ecosystem services into complementary, supplementary or competitive. We use a flexible transformation function and cross-sectional data on 120 surveyed smallholder farmers with agroforestry certification in rural Mount Kenya. The results suggest that the joint production (of agricultural output and ecosystem services) for a substantial number of smallholder farms in Kenya may not show a complementary relationship. The biophysical linkage between marketed outputs and ecosystem services strongly influences the marginal cost of ecosystem services. PES schemes could become more efficient if they would target smallholder farms based on the aforementioned classifications by offering a range of contracts to encourage competitive bidding.

Intraspecific variation in feeding strategies of Galapagos sea lions: A case of trophic specialization.

The trophic behavior of marine predators varies according to the level of competition to which they are exposed. In general, populations that inhabit lower productivity systems face a strong intraspecific competition, which contributes to the development of different foraging strategies to maximize nutritional efficiency. Given the high trophic flexibility of Zalophus wollebaeki, this species is considered appropriate for the analysis of such behavior. Furthermore, this trophic flexibility has allowed them to persist in a seemingly marginal ecosystem. In this study, we used a comparative analysis of variables (diet and dive behavior) related to Z. wollebaeki trophic niche plasticity to better understand their foraging ecology, using techniques such scat analysis, satellite telemetry and complementarily an isotopic analysis. Scat analysis revealed intra-population variation in their diet, represented by prey from different environments (epipelagic and benthic). These results are supported by the animals’ locations at sea and diving profiles. Global Positioning System (GPS) and time-depth recorder (TDR) records showed the existence of two groups, with differing feeding areas and diving behavior. Also the δ15N values showed differences in the trophic level at which the species fed. These results constitute a relevant finding in the evolutionary behavior of the species, showing that Z. wollebaeki has developed a high degree of foraging flexibility, thus increasing its survival rate in an ecosystem that is highly demanding in terms of resource availability.

Functional Gene Diversity and Metabolic Potential of the Microbial Community in an Estuary-Shelf Environment.

Microbes play crucial roles in various biogeochemical processes in the ocean, including carbon (C), nitrogen (N), and phosphorus (P) cycling. Functional gene diversity and the structure of the microbial community determines its metabolic potential and therefore its ecological function in the marine ecosystem. However, little is known about the functional gene composition and metabolic potential of bacterioplankton in estuary areas. The East China Sea (ECS) is a dynamic marginal ecosystem in the western Pacific Ocean that is mainly affected by input from the Changjiang River and the Kuroshio Current. Here, using a high-throughput functional gene microarray (GeoChip), we analyzed the functional gene diversity, composition, structure, and metabolic potential of microbial assemblages in different ECS water masses. Four water masses determined by temperature and salinity relationship showed different patterns of functional gene diversity and composition. Generally, functional gene diversity [Shannon–Weaner’s H and reciprocal of Simpson’s 1/(1-D)] in the surface water masses was higher than that in the bottom water masses. The different presence and proportion of functional genes involved in C, N, and P cycling among the bacteria of the different water masses showed different metabolic preferences of the microbial populations in the ECS. Genes involved in starch metabolism (amyA and nplT) showed higher proportion in microbial communities of the surface water masses than of the bottom water masses. In contrast, a higher proportion of genes involved in chitin degradation was observed in microorganisms of the bottom water masses. Moreover, we found a higher proportion of nitrogen fixation (nifH), transformation of hydroxylamine to nitrite (hao) and ammonification (gdh) genes in the microbial communities of the bottom water masses compared with those of the surface water masses. The spatial variation of microbial functional genes was significantly correlated with salinity, temperature, and chlorophyll based on canonical correspondence analysis, suggesting a significant influence of hydrologic conditions on water microbial communities. Our data provide new insights into better understanding of the functional potential of microbial communities in the complex estuarine-coastal environmental gradient of the ECS.

ANALYSIS OF GINGER FARMING BUSINESS IN PEAT LAND IN WEST KALIMANTAN (Case study: Ginger Farmer in Pasir Palembang Village, Mempawah Regency)

<p align="center"><strong>ABSTRACT</strong></p><p align="center"> </p>Peat land potential as agricultural land in Indonesia is quite large of about 6 million hectaresout of 21 million hectares or 11% of land area in Indonesia. The utilization of peat land as agricultural land requires accurate and careful planning, appropriate technological application, and proper management because of its marginal and fragile ecosystem. Peat land has a big potential as agricultural land because this land contains high organic material. Theproblem is that the pH is very low so that it is not good for agricultural land. However, the research in Pasir Palembang Village, Mempawah Regency proved that doing ginger farming business in peat land could increase the farmers’ income and welfare. The research aims to: 1) study problems faced in doing ginger farming business, 2) know ginger farmer’s income. Data collection was done by using Focus Group Discussion method and R/C Ratio analysis. The research result found out that the main problem in ginger farming business was that of rotten tuber. Based on the analysis result it was found that R/C ratio obtained in ginger farming business was 3.4. Total revenue obtained was Rp75,000,000with the profit over cash expense was Rp53,620,000 and the profit over total expense was Rp53,470,000. Theresearch concluded that: 1) there need be a serious handling of rotten tuber disease in ginger plant, 2) doing ginger farming business could increase income and welfare of the peat land farmers in Pasir Palembang Village, Mempawah Regency.