Manure Resources Research Articles

The Effect of Manure Application Rates on the Vertical Distribution of Antibiotic Resistance Genes in Farmland Soil

Manure application is the primary input route for antibiotic resistance genes (ARGs) in farmland soil. This study investigated the effects of varying the rates of five chicken manure applications on the accumulation and distribution of ARGs across different soil depths (0–20, 20–40, and 40–60 cm) using metagenomic sequencing. The results revealed that the distribution of ARGs in farmland soil was closely linked to soil depth and influenced to some extent by the fertilizer quantity after 30 days of fertilization. ARGs were predominantly concentrated in the surface soil and exhibited a significant decrease in type and abundance with an increased soil depth. Compared with soil treated with chemical fertilizers alone, chicken manure-treated surface soil presented a higher diversity and abundance of ARGs. However, the diversity and abundance of ARGs did not increase proportionally with the increasing ratios of chicken manure application (0, 25, 50, 75, and 100%). ARGs in soil primarily conferred resistance to host bacteria through antibiotic efflux pumps (~33%), antibiotic target alteration (~31%), antibiotic inactivation (~20%), and antibiotic target protection (~8%). Correlation analysis involving ARGs and soil microorganisms revealed widespread multidrug resistance among soil microorganisms. Furthermore, two genera of human pathogenic bacteria (Pseudomonas sp. and Listeria sp.) were identified as potential microbial hosts of ARGs in all treatments. Correlation analysis involving ARGs and environmental factors indicated that soil ARGs are predominantly influenced by heavy metals and microorganisms. This paper offers valuable insights for environmental risk assessments regarding the utilization of livestock manure resources. Additionally, it furnishes a scientific foundation for farmland application strategies pertaining to livestock manure.

Manure, Environmental Impact, and Soil Health: Technologies, Application, and Benefits

AbstractThis is the fourth article in our series on soil health and greenhouse gas emissions on U.S. dairies associated with crop production. In this article, we’ll look at innovative manure products that might help dairy farmers better manage their manure resources and answer some key questions. Earn 1 CEU in Sustainability by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.

Impacts of long-term organic manure inputs on cultivated soils with various degradation degrees

With an increasing concern of soil health and sustainable development, organic manure application has been readdressed in recent years. In Northeast China, the agricultural industry is highly developed and organic manure resources are abundant, but soil degradation is serious due to extensive expansion of cultivation. Currently, limited information is available on how soils with various degradation degrees respond to long-term manure fertilization. Given that, a comparative study was conducted based on soil functional assessment of croplands with over 35 years of manure fertilization (maize straw compost, SA; cattle manure, CA) and various degradation degrees (none, light, moderate, and heavy). Soils in these croplands were loess-derived Mollisols and concentrated on a gentle to flat slope. Except for the difference in manure fertilization (SA and CA), similar field managements were adopted for these croplands during the past 35 years. Results showed that the impacts of erosion degradation and manure fertilization on soil physical structure lasted across the whole profile (0 ∼ 120 cm), while their impacts on soil nutrient properties were concentrated in shallow layers (<50 cm). Meanwhile, erosion degradation dominated 17.9% of the variance of soil physical structure, and soil profile depth contributed to 15.9% of soil nutrient variation. According to field validation regarding soil erosion modulus and crop yield, principal component analysis (PCA) with a nonlinear scoring method showed a good performance on the integrated soil functional index (ISI) assessment in this study area. The ISI of these croplands was found to be minimally characterized by BD, SOC, TN, AK, sand content, and CEC (R2 >0.64, p < 0.001). In addition, manure fertilization could significantly improve the ISI and maize yield for degraded soils, but had a slight impact on these two indicators for non-degraded soils. In terms of contribution to ISI improvement, SA (1.79% ∼ 283.73%) generally outperformed CA (4.53% ∼ 212.83%), while the latter was more effective in altering soil structure in deep layers (>70 cm). Generally, the ISI and maize yield were more susceptible to erosion degradation than manure fertilization. The obtained results provide useful information for a better cropland management in Northeast China.

Life Cycle Environmental and Economic Assessment of Different Biogas and Biogas Residue Operation Models

The utilization of biogas and biogas residues is an important objective of animal manure resource management. Regarding the environmental friendliness and economic suitability of utilization modes, previous studies have evaluated and compared biogas or biogas residue individually, which may lead to incomplete environmental and economic assessments. In this study, the environmental and economic aspects of six integrated biogas and digestate operation modes, i.e., biogas utilization (direct combustion, cogeneration, and purification) and biogas residue utilization (field return and composting), were analyzed via life cycle assessment (LCA) and the net present value (NPV) method, with an animal manure biogas plant in Tangshan City as the study object. The results of LCA showed that biogas cogeneration + biogas residue composting disposal was superior to other models in most environmental indicators. NPV analysis showed that direct biogas combustion + biogas residue composting was the best, breaking even in the 3rd year with a cumulative profit of 250 million CNY. With increased environmental costs and benefits, the biogas cogeneration + biogas residue composting model had the best performance in terms of efficiency ratio at 2.58; the NPV of both operating models of biogas cogeneration increased, while the NPV of the other operating models decreased about 20%.

Risk assessment and dissemination mechanism of antibiotic resistance genes in compost

In recent years, the excessive of antibiotics in livestock and poultry husbandry, stemming from extensive industry experience, has resulted in the accumulation of residual antibiotics and antibiotic resistance genes (ARGs) in livestock manure. Composting, as a crucial approach for the utilization of manure resources, has the potential to reduce the levels of antibiotics and ARGs in manure, although complete elimination is challenging. Previous studies have primarily focused on the diversity and abundance of ARGs in compost or have solely examined the correlation between ARGs and their carriers, potentially leading to a misjudgment of the actual risk associated with ARGs in compost. To address this gap, this study investigated the transfer potential of ARGs in compost and their co-occurrence with opportunistic pathogenic bacteria by extensively analyzing metagenomic sequencing data of compost worldwide. The results demonstrated that the potential risk of ARGs in compost was significantly lower than in manure, suggesting that composting effectively reduces the risk of ARGs. Further analysis showed that the microbes shifted their life history strategy in manure and compost due to antibiotic pressure and formed metabolic interactions dominated by antibiotic-resistant microbes, increasing ARG dissemination frequency. Therefore, husbandry practice without antibiotic addition was recommended to control ARG evolution, dissemination, and abatement both at the source and throughout processing.

Sustainable assessment and resource recycling opportunities identification for China's pig industry: Integrating environmental, economic and social perspectives

Livestock plays a vital role in the livelihoods and nutritional security of people worldwide and leads to significant environmental challenges. Manure management is a major hurdle for sustainable livestock development and serves as a critical source of natural fertilizer and green energy. Given that pork accounts for the highest meat production and consumption share, this study systematically evaluates China's pig production and main manure resource utilization scenarios from life-cycle environmental, economic, and social perspectives. Results show that China's pig production poses considerable potential environmental risks, primarily in the categories of fossil depletion, freshwater ecotoxicity, particulates formation, global warming, and water depletion. Feed production coupled with traditional manure treatment scenario contributes to 94.4 %–98.4 % of the key environmental impact categories and socioeconomic impact, with 32.0 % to 38.3 % of the economic costs also stemming from feed costs. In the energy utilization scenario, the potential benefits of fossil depletion and global warming categories resulting from the replacement of synthetic fertilizers with manure-based organic fertilizers in the traditional pig manure treatment scenario could be expanded by 89.6 % and 39.2 %, respectively. China's pig production contributed 0.72 % of the national carbon emissions in 2020. Fully utilizing energy potential and organic fertilizer from pig manure would achieve 0.63 % of national carbon reduction potential, as well as an economic benefit of $1.45 × 1012 and a social benefit of $8.89 × 109. Precision feeding technology, rebuilding the spatial connection between pig farms and arable land, and internalizing external costs were recommended to ensure the sustainable development of China's pig industry.

Soybean isoflavones and flavonoids of Artemisia argyi enhanced conversion of chicken manure by black soldier fly (Hermetia illucens L.) larvae and effects on microbiota of their larvae and residues

Black soldier fly composting technology is a promising technology for the recovery of nutrients from organic waste and a hot spot in the research field of animal manure resource utilization, the co-transformation of microorganisms is not obvious in practical application, which limits the development of composting technology and results in the limited value of its products, therefore, plant flavonoids, which may have the effect of promoting the transformation of larvae, were selected as additives to promote the transformation of black soldier fly. In this study, the promoting effect of soybean isoflavones and flavonoids of Artemisia argyi on the conversion of black soldier fly was demonstrated, the important factors (larvae weight, total nitrogen, crude protein, conversion rate) in the composting system were determined and the changes in the microbial community structure during the composting process were clarified. The results have shown that 40 mg/kg soybean isoflavones and flavonoids of Artemisia argyi could significantly improve the larvae weight, and the black soldier fly larvae treated by 20 mg/kg flavonoids of Artemisia argyi could dramatically improve the conversion efficiency of crude protein in chicken manure (p < 0.05). Soybean isoflavones flavonoids and flavonoids of Artemisia argyi could significantly change the microbiota composition of residues (p < 0.05), while had no significant effect on the larvae microbiota. Soybean isoflavones flavonoids might promote the conversion of crude protein in chicken manure by the black soldier fly larvae through regulating their intestinal microbiota. The study provided reference data for using the flavonoids of Artemisia argyi to promote crude protein transformation of black soldier fly larvae from chicken manure as feed protein substitute.

Improvement of carbon source composition reduces antibiotic resistance genes in the ectopic fermentation system

Effectively reduce antibiotic resistance genes (ARGs) in ectopic fermentation system (EFS) is essential for practical production. In this study, three experiments were performed to explore how to remove ARGs in EFS effectively. Results demonstrated that ARGs were easily enriched in rice-husk-sawdust padding; simultaneous addition of laccase and cellulase suppressed the ARGs, mainly by increasing soluble carbohydrate concentration and promoting humic acid concentration; addition of corn stalks into rice-husk-sawdust decreased the abundance of ARGs by improving the carbon source structure and enhancing cellulase activity. In conclusion, the present study provides a guidance to reduce the threat of ARGs in EFS, which paved a potential pathway to safely utilize manure resources.

Distribution of livestock sectors in Canada: Implications for manureshed management.

Canada's livestock production and human populations are concentrated in southern regions. Understanding spatial and temporal distributions of animals and excreted nutrients is key to optimizing manure resources and minimizing impact of livestock. Here, we identify manureshed concerns and opportunities by reconciling nitrogen supply and demand on a regional and national scale. Data based on national statistics and farm surveys were allocated to homogeneous soil polygons (Soil Landscapes of Canada [SLC]) to quantify changes in nutrient distribution and ammonia (NH3 ) emissions across Canada (1981-2018). Livestock sectors tied to domestic consumption, dairy and poultry, were stable over time and well dispersed. Export driven beef production has moved west since 1981, whereas pig production was prominent in Manitoba, Quebec, and Ontario. Per ha manure N excretion across livestock sectors in 2018 was generally low with 58% and 6% of the SLCs averaging <25 and >100kgNha-1 , respectively. Although only 3% of SLCs had average NH3 emissions reaching 16-200kgha-1 , most of these were located near cities and emissions spiked in spring when more people might be exposed. The greatest concentrations of nutrients and livestock occurred around the three largest metropolitan areas: Toronto, Montreal-Quebec City, and Vancouver, posing challenges for nutrient recycling and public health. This study shows that as Canadian cities and livestock agriculture grow in southern Canada, so will challenges around food production, human health, and managing nutrients. Livestock and land use strategies are needed to reconcile changing animal sectors and growing populations.

Design of Farm Livestock Manure Resource Management Platform Based on Intelligent Semantic Analysis of Real-Time Monitoring Images

With the rapid development of big data and 5G communication, the management of agricultural and livestock manure resources in rural areas has also embarked on a high-tech monitoring and platform design route. First, starting from the impact of pig manure on the environment, it analyzes how to use real-time monitoring images to semantically analyze the management and resource utilization of pig manure. According to the needs of building an intelligent behavior analysis system for agricultural storage ponds, first of all, establish a system that can not only express the purpose of use by farmers, but It can also express the direction of the construction of modern rural areas. Finally, based on the target positioning, identification and tracking in the dynamic scene, the deep processing and comprehensive development and utilization of feces are carried out, and the environmental management and resource development are combined. The results show that: from the current situation, the resource utilization of chicken manure mainly has two aspects, one is as feed, the other is as fertilizer; the resource utilization of pig manure and cow manure is suitable for chemical fertilizer utilization.

Disentangling the heterogeneous effects of different support policies on livestock and poultry farmers’ willingness to utilize manure resources: Evidence from central China

China’s livestock and poultry industries have undergone massive transformations, with far-reaching implications for resource consumption and environmental issues. Utilizing waste resources from livestock and poultry, which requires increased farmer participation, is critical for China to meet its goal of lowering carbon emissions while also advancing high-quality animal husbandry. To this end, this study develops a model based on stimulus-organism-response (SOR) theory to explore the impact of different policy instruments on farmers’ willingness to utilize resources, as well as the mediating effect of farmers’ perception and the moderating effect of farmers’ attitude toward risk. Using OLS and Bootstrap estimation on survey data from 607 farmers from 11 counties in China’s Hunan province, this study reaches the following main results. First, incentive policies have a significant positive effect on farmers’ willingness to utilize resources. The service policy has the greatest positive impact among the various incentive policies considered, followed by the subsidy policy, while the impact of the propaganda policy is insignificant. Second, farmers’ perceptions mediate the effect of incentive policies on their willingness to utilize resources. Finally, risk attitude negatively impacts farmers’ willingness to utilize resources, indicating that the more conservative a farmer’s risk preference, the greater the impact of farmers’ perception on willingness to utilize resources, and vice versa. To achieve sustainable livestock production, policymakers should prioritize measures that can improve and strengthen regulatory control, encourage education and technology adoption related to resource utilization, and offer subsidies for manure treatment and utilization.

Water Self-Purification with Zero External Consumption by Livestock Manure Resource Utilization.

Improper disposal of waste biomass and an increasing number of emerging contaminants (ECs) in water environment are universal threats to the global environment. Here, we creatively propose a sustainable strategy for the direct resource transformation of livestock manure (LM) into an innovative catalyst (Fe-CCM) for water self-purification with zero external consumption. ECs can be rapidly degraded in this self-purification system at ambient temperature and atmospheric pressure, without any external oxidants or energy input, accompanied by H2O and dissolved oxygen (DO) activation. The performance of the self-purification system is not affected by various types of salinity in the wastewater, and the corresponding second-order kinetic constant is improved 7 times. The enhanced water self-purification mechanism reveales that intermolecular forces between anions and pollutants reinforce electron exchange between pollutants and metal sites on the catalyst, further inducing the utilization of the intrinsic energy of contaminants, H2O, and DO through the interfacial reaction. This work provides new insights into the rapid removal of ECs in complicated water systems with zero external consumption and is expected to advance the resource utilization of livestock waste.

The effect of perceived value on farmers' livestock manure resource utilization behavior: Evidence from Shandong, China.

The rapid development of animal husbandry has brought many problems such as ecological environmental pollution and public health damage. The resource utilization of livestock manure is the key way to deal with the above crisis and turn waste into treasure. Based on the theory of perceived value, this paper uses multi-group structural equation model to explore the driving mechanism of perceived value on the resource utilization behavior of livestock manure. The results showed that: (1) The resource utilization behavior of livestock manure followed the logic of "cognitive level → cognitive trade-off → perceived value → behavioral intention → behavioral performance." Perceived benefit and perceived risk have positive and reverse driving effects on perceived value, respectively. Perceived value has a positive driving effect on behavioral intention. The behavioral intention has a positive driving effect on utilization behavior. (2) Among the observed variables of perceived benefits, ecological benefits have the greatest impact; Among the observed variables of perceived risk, economic risk has the greatest impact. Among the observed variables of perceived value, Significance cognition has the greatest influence. Among the observed variables of behavioral intention, utilization intention has the greatest influence. (3) The perceived value has a differential effect on the utilization behavior of livestock manure resources of different part-time farmers, and the driving effect is more obvious for full-time farmers. Therefore, it is necessary to improve the resource utilization system of livestock manure, increase the channel for realizing the output of manure resources, strengthen technical assistance and policy subsidies, and implement policies according to local conditions to improve the overall perceived value of farmers.

Evaluating water quality benefits of manureshed management in the Susquehanna River Basin.

Manureshed management guides the sustainable use of manure resources by matching areas of crop demand (nutrient sinks) with areas generating livestock manure (nutrient sources). A better understanding of the impacts of manureshed management on water quality within sensitive watersheds is needed. We quantified the potential water quality benefits of manureshed-oriented management through scenario-based analyses in the Susquehanna River Basin (SRB) using the Soil and Water Assessment Tool. Five manureshed management scenarios were developed and compared with a baseline "business-as-usual" scenario. The baseline assumes manure is less transportable, which means some locations have manure application in excess of crop demand. The "watershed nutrient balance" scenarios assume excess manure from surplus locations is transportable and that manure is applied around the SRB based on crop nutrient demand. The "watershed nutrient balance avoiding runoff prone areas" scenarios assume manure is transportable but not applied in vulnerable landscapes of the SRB. Each scenario was evaluated under two application rates considering crop nitrogen demand (N-based) and phosphorus demand (P-based). Phosphorus-based manureshed management was more effective in water quality improvements than N-based management. Phosphorus-based nutrient balance scenarios simulated 3 and 25% reduction in total N (TN) and total P (TP), respectively, from the baseline scenario at the watershed outlet. The N- and P-based scenarios avoiding runoff prone areas simulated 3 and 6% reduction in TN loss and 4 and 25.2% reduction in TP loss, respectively, from the baseline. Overall, the manureshed management scenarios were more effective in improving the quality of local streams in livestock-intensive regions than at the watershed outlet.

Estimation of short- and long-term floor and ceiling prices for manure in a crop and livestock farms exchange

Abstract Organic matter is a key element of soil fertility. However, in-soil stocks of organic matter are in decline in specialized crop farms. Reintroducing organic fertilizers could be a way to increase or at least maintain organic matter stocks in these soils. Straw/manure exchanges between crop farms and livestock farms could improve overall land fertility and thus the long-term income of farmers. Here we used a bioeconomic model to estimate the agronomic and economic benefits of straw/manure exchanges as part of a strategy to improve soil fertility. Iterative simulations were run with prices of farmyard manure varying from €0 to €20 ton−1 to identify the price at which a crop farm can buy manure and sell straw without degrading its net income (manure ceiling price) compared to purchasing mineral fertilizer only and ploughing back straw. Parallel simulations were run to identify the price at which a livestock farmer can sell manure and buy straw without degrading its net income (manure floor price) compared to keeping all manure on the farm and buying straw on the market. The key new contributions of this study are that it (i) considers the characteristics of manure beyond its short-term fertilizing value, i.e., better mineralization of humus and a 10% increase in crop yields, (ii) estimates both the economic and agronomic benefits of manure and (iii) assesses the impacts of uncertainties on manure effects and prices. The results show that it is profitable for a French farmer in a conventional field crop system to buy manure at a price between €10 ton−1 in the short term and €18 ton−1 in the long term. The results also show that it is still economically advantageous for a livestock farmer to sell part of their manure, even at a very low price. This study shows that it is possible to better distribute manure resources over a territory in a way that enriches the soils of crop farms without degrading the soils of livestock farms.

Adoption of manure resource utilization technologies and its economic effects on large-scale dairy farms

Adoption of manure resource utilization technologies and its economic effects on large-scale dairy farms

Effects of chicken manure substitution for mineral nitrogen fertilizer on crop yield and soil fertility in a reduced nitrogen input regime of North-Central China.

Organic manure has been proposed to substitute part of the chemical fertilizers. However, past research was usually conducted in regimes with excessive nitrogen (N) fertilization, which was not conducive to the current national goal of green and sustainable development. Therefore, exploring the potential of organic fertilizer substitution for mineral N fertilizer under regimes with reduced N inputs is important to further utilize organic fertilizer resources and establish sustainable nutrient management recommendations in the winter wheat (Triticum aestivum L.) - summer maize (Zea mays L.) rotation system in North-central China. In this study, a 4-year field experiment was conducted to investigate the effects of different chicken manure substitution ratios on crop yield, N recovery efficiency (REN), soil N and soil organic matter contents, to clarify the optimal organic substitution ratio of N fertilizer under reduced N application (from 540kg N ha-1 year-1 to 400kg N ha-1 year-1). Six substitution ratios were assessed: 0%, 20%, 40%, 60%, 80% and 100% under 200kg N ha-1 per crop season, respectively, plus a control with no N application from chemical fertilizer or chicken manure. Results showed that the highest yield was achieved under the 20% substitution ratio treatment, with 1.1% and 2.3% higher yield than chemical N alone in wheat season and maize seasons, respectively. At the chicken manure substitution ratios of 20% in wheat season and 20%-40% in maize season, the highest REN reached to 31.2% and 26.1%, respectively. Chicken manure application reduced soil residual inorganic N with increasing substitution ratio. All organic substitution treatments increased soil organic matter and total N content. Implementing 20% organic substitution in wheat season and 20%-40% in maize season under the reduced N application regime in the North-central China is therefore recommended in order to achieve high crop yields and REN, improve soil fertility and enhance livestock manure resource utilization.

The potential for livestock manure valorization and phosphorus recovery by hydrothermal technology - a critical review

Carbon neutrality has become one of the major challenges to the global sustainable development. The sector of livestock & manure, which accounts for 5.8% of greenhouse gas emissions, has attracted worldwide attentions. The implemention of sustainable technologies is considered an important step towards achieving the carbon reduction in livestock & manure sector. In this work, the recent trends in the potential for livestock manure valorization and phosphorus (P) recovery by hydrothermal technology were systematically studied. First of all, the current situation of livestock manure treatment and waste P utilization at domestic and international level were reviewed. The strengths and challenges of several technical means of livestock manure resource utilization, include composting, anaerobic digestion, and thermochemical conversion, were discussed. Then, the research advance of hydrothermal technology for P utilization and two techniques, include precipitation and adsorption methods, were summarized. Finally, the research perspective of waste P utilization was covered. This review provides the critical strategy for the utilization of livestock manure and waste P resource. Future research priorities in the sustainable management of carbon neutrality in these settings are also proposed.

From manure to high-value fertilizer: The employment of microalgae as a nutrient carrier for sustainable agriculture

The use of livestock and poultry manures as fertilizers is regarded as an important action in the development of circular agriculture. However, long-term fertilization with manure is accompanied with heavy metals accumulation, pathogens contamination, and ammonia toxicity, which may cause the decline of soil quality and crop productivity. To address the aforementioned problems, in this work, we proposed a “manure-algae-crop” technical route, in which microalgae are employed as a carrier transporting nutrients in manures to soil and crops. This paper presented the advanced techniques which can tackle with the hazardous factors in manures and promote the nutrients assimilation by microalgae. Additionally, the utilization of microalgae in the forms of living cyanobacteria , liquid fertilizer , and slow-release fertilizer was discussed in detail. Particularly, we also focused on the disadvantages of algae fertilizers in different forms. By the end of this work, based on the analysis of current challenges and problems, we discussed the potential solutions and prospects of “manure-algae-crop” technical route. In fact, previous studies have achieved many progresses in algae-based manure remediation and utilization of algae fertilizers. Hence, based on current knowledge, “manure-algae-crop” technical route has greater advantages over traditional models of manures utilization in agriculture. In the foreseeable future, with the development of new technologies, reduction of operational cost, and upgrading of agricultural model, “manure-algae-crop” technical route will be applied more frequently in agriculture. It is expected that this technical route can truly promote the high-value utilization of manures and the development of eco-friendly agriculture. • Manure contains hazardous factors threatening soil quality and crop cultivation. • The “manure-algae-crop” technical route is proposed to utilize manure resource. • Algae serve as a carrier transporting nutrients in manure to soil and crop. • Algae in different forms can be employed for soil quality improvement. • Some challenges must be addressed for the practical application of algae fertilizer.

Redeploy manure resources to enhance the agro-pastoral cycle

Returning manure to the land is a critical link in the internal cycle of agricultural systems, but excess manure leads to water eutrophication. The traditional manure re-use method brings pathogenic microorganisms, heavy metals, antibiotic resistance genes (ARGs), insect eggs, and other contaminants into the soil, posing a great threat to the ecological environment and human health. Clarifying the spatial distribution patterns of manure nutrient supply and farmland nutrient demand can help guide a more efficient and harmless way to return manure to farmland. This work counted data on cultivation and breeding in 356 cities on the Chinese mainland from 2015 to 2019 and calculated the livestock breeding volume (LB), total environmental capacity (C), and remaining environmental capacity (RC) accordingly. The Spatial Autocorrelation Model (SAC) was used to analyze the distribution patterns of the three. Data results show that China currently has the potential to double LB, but most cities in the west have excess manure due to the mismatched distribution of LB and C. The hot spot analysis results demonstrate the priority/general areas of manure management and the export/import areas of manure resources. The results of the outlier analysis show that some cities located at the boundary of RC Cold/Hot spot areas (e.g., Chengdu City) can perform resource replacement nearby to relieve local environmental pressure. This study analyzes the potential and realistic resistance to utilizing manure as an organic nutrient resource and provides a reference for developing manure management links.