Turbulent jet ignition (TJI) effectively achieves lean and stable combustion in hydrogen engines. However, research on injection strategies for TJI hydrogen engines is still lacking. In this study, experimental methods investigated the effects of single and split injection strategies on the combustion and emissions of TJI hydrogen engines under medium loads. The numerical simulation reveals the operational characteristics of fuel distribution, ignition capability, and energy conversion in the pre-chamber at different injection times in a single injection strategy. This work is conducted at 1600 rpm with a manifold absolute pressure of 70 kPa. The experimental results indicate that the delay of the start of injection (SOI) can enhance the performance of the TJI hydrogen engine. However, as SOI approaches TDC, emissions worsen, and combustion stability decreases. When SOI is 90°CA BTDC, the brake mean effective pressure (BMEP) and brake thermal efficiency (BTE) can achieve 3.1 bar and 32.9 %, with the coefficient of variation of the IMEP (COVIMEP) of 1.6 % and lower emissions. For the split injection strategy, delaying the secondary end of injection (SEOI) can increase BMEP and BTE. As SEOI gradually delays the TDC, emissions deteriorate sharply. Under the split injection strategy, COVIMEP is higher, which is unfavorable for hydrogen engine applications.