BackgroundTraditional mandibular reconstruction has relied on the use of vascularized and non-vascularized autografts. The use of allografts and tissue engineering modalities has risen as an alternative. PurposeThe purpose of this study was to determine the success of a cellular bone matrix (CBM) allograft composed of lineage committed bone forming cells for mandibular tissue engineering and reconstruction. Study design, setting, sampleA retrospective cohort study was implemented using data from subjects treated with a CBM at the University of Louisville from 2019 to 2023. Subjects were excluded if they were not treated with a CBM, data were not complete, or postoperative follow-up time was less than 3 months. Predictor variablesThe predictor variables were composed of heterogenous variables grouped into the following categories: demographics (age, sex), medical history (history of penicillin (PCN) allergy, history of diabetes mellitus (DM) and tobacco use), etiology (benign tumor, ballistic trauma, non-ballistic trauma, odontogenic cyst, osteomyelitis/MRONJ), mandibular resection length (cm) and type (marginal, segmental), delayed vs immediate reconstruction, and whether an autograft (proximal tibia, TBG) with platelet rich fibrin (PRF) was used in combination with the CBM. Main outcome variableThe primary outcome variable was graft success (yes or no). Success was defined as bony union and defect fill (demonstrated on panoramic radiograph) and mandibular stability (based on postoperative clinical examination at 3 months). CovariatesNot applicable. AnalysesDescriptive statistics were calculated for each variable. To measure the associations between the risk factors and graft success, Fisher’s exact test for categorical variables and the Wilcoxon rank sum test for numeric data were used. A p-value of <0.05 was considered significant. ResultsThe sample included 38 subjects. The median age of all subjects was 46 (IQR 32.6) years. Overall, 28 (73.7%) cases were successful. Subjects with a reported PCN allergy or a history of DM had significantly lower success (2, 7.1% with PCN allergy or DM) compared to those who did not (p = 0.008, PCN allergy; p = 0.03, DM). Conclusions and RelevanceThis is the largest case series of CBM based mandibular reconstruction relative to the available maxillofacial surgery literature. The clinician should consider confirmation of penicillin allergy so penicillin-type antibiotics can be used. CBMs may be an alternative to autografts.