Management Of Related Diseases Research Articles

Enzyme-Driven LC-HRMS Approach for Specific Recognition of 12α-Hydroxy Bile Acids.

The synthesis of 12α-hydroxylated bile acids (12HBAs) and non-12α-hydroxylated bile acids (non-12HBAs) occurs via classical and alternative pathways, respectively. The composition of these BAs is a crucial index for pathophysiologic assessment. However, accurately differentiating 12HBAs and non-12HBAs is highly challenging due to the limited standard substances. Here, we innovatively introduce 12α-hydroxysteroid dehydrogenase (12α-HSDH) as an enzymatic probe synthesized by heterologous expression in Escherichia coli, which can specifically and efficiently convert 12HBAs in vitro under mild conditions. Coupled to the conversion rate determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS), this enzymatic probe allows for the straightforward distinguishing of 210 12HBAs and 312 non-12HBAs from complex biological matrices, resulting in a BAs profile with a well-defined hydroxyl feature at the C12 site. Notably, this enzyme-driven LC-HRMS approach can be extended to any molecule with explicit knowledge of enzymatic transformation. We demonstrate the practicality of this BAs profile in terms of both revealing cross-species BAs heterogeneity and monitoring the alterations of 12HBAs and non-12HBAs under asthma disease. We envisage that this work will provide a novel pattern to recognize the shift of BA metabolism from classical to alternative synthesis pathways in different pathophysiological states, thereby offering valuable insights into the management of related diseases.

The Advancement of Nanomaterials for the Detection of Hepatitis B Virus and Hepatitis C Virus.

Viral hepatitis is a global health concern mostly caused by hepatitis B virus (HBV) and hepatitis C virus (HCV). The late diagnosis and delayed treatment of HBV and HCV infections can cause irreversible liver damage and the occurrence of cirrhosis and hepatocellular carcinoma. Detecting the presence and activity of HBV and HCV is the cornerstone of the diagnosis and management of related diseases. However, the traditional method shows limitations. The utilization of nanomaterials has been of great significance in the advancement of virus detection technologies due to their unique mechanical, electrical, and optical properties. Here, we categorized and illustrated the novel approaches used for the diagnosis of HBV and HCV.

A review on the chemical constituents and pharmacological efficacies of Lindera aggregata (Sims) Kosterm.

Lindera aggregata (Sims) Kosterm. (L. aggregata), which belongs to the genus Lindera in the family Lauraceae, is widely distributed in Asia and the temperate, tropical regions of North America. Its roots and leaves have been used for thousands of years as traditional Chinese medicine and/or functional food. To further explore its underlying nutritional value, this review provided a comprehensive insight into chemical constituents and pharmacological effects on L. aggregata. The phytochemical investigation of different parts of L. aggregata led to the identification of up to 349 components belonging to sesquiterpenoids, alkaloids, flavonoids, essential oils, and other compounds. Among them, sesquiterpenoids, flavonoids, and alkaloids are assessed as representative active ingredients of L. aggregata. A wide variety of pharmacological effects of L. aggregata, such as anti-hyperlipidemic, anti-tumor, anti-inflammatory, analgesic, and anti-oxidant, have been proved in vitro and in vivo. In summary, this review aims to provide a scientific basis and reference for further research and utilization of L. aggregata and lay the foundation for developing functional foods with potential active ingredients for the prevention and management of related diseases.

Management of nerve injuries by utilization of nutraceuticals

Nerve injuries leads to loss of function from different organs of patient. It is caused due to dysfunction of neural cell. This dysfunction can occur by different ways like of genetics, protein accumulation in neural cell or any of the sudden accident. The nerve consist of various parts and one axon. The same nerve divides as CNS part and PNS part. The regeneration of nerve axon in PNS can be possible to function but the CNS part of axon cannot be regenerated. This regeneration takes place takes place with injured cell. This injury leads to pain referred as neuropathic pain. The nerve cell injury can be putted into three categories as of Neuropraxia, Axonotmesis and Neurotmesis. The injured neural cell leads to neurodegeneration. There are several treatments available on the basis of nutrients and constituents taken from herbal medicinal plants. Nutraceuticals is a branch known by its food supplements and nutrients of any herbal plant which have positive effect towards patient health and management of related disease. Nutraceuticals have less side and adverse effect towards patient. There are some medications which is derived from herbal medicinal plant such as Ginseng, Ashwagandha, Bacopa monnieri, Ginkgo biloba, Centella asiatica. Some compounds belong to as class of flavonoids, celastrol, trehalose, lycopene, sesamol, resveratrol and curcumin are showing high positive impression. The present scenario demands for the herbal medicinal treatment and for the fulfilment of this need the approach towards nutraceutical are raising high. Neuroprotection mechanism also shown by these nutrients. Apart from neuro regeneration, neuroprotective effect the nutraceutical also show antioxidant, anti-inflammatory effect. Polyphenol compound help to increase neural function.

A Multicalibration Urea Potentiometric Sensing Array Based on Au@urease Nanoparticles and Its Application in Home Detection.

Home potentiometric sensing devices can real-time monitor personal health status and are widely used in the prevention and management of related diseases. However, variations in the composition and the pH of the sample matrix tend to change the basic potential and response slope of some potentiometric sensors, thus affecting detection reliability. Therefore, this work uses the detection of urea in urine as a model to improve reliability of the potentiometric sensor in home detection. Au@urease nanoparticles were synthesized as the sensing material to improve the stability of the urease-based potentiometric sensor. Meanwhile, a multicalibrated urea potential (MCUP) sensing array was designed, which consists of a urea electrode group, a pH electrode group, and a reference channel. The urea electrode group and the pH electrode group contain respectively a sensing channel and a calibration channel. The basic potential of sensing channels can be calibrated through the corresponding calibration channels. Moreover, the pH electrode group can not only measure the pH values of the samples but also calibrate the response slope of the urea electrode group through the calibration coefficient, thus improving the reliability of home detection. Consequently, the potentiometric sensing array based on the enzyme reaction can be applied in body fluids with a wide pH range.

Assessment and Management of Loose Bodies in the Knee Joint and Related Disease: A Narrative Review.

Loose bodies are frequently encountered during clinical activity and are a common finding during knee arthroscopy. Usually, treatment consists of the removal of loose bodies, which can be challenging even for experienced surgeons. The excision alone is not always the complete treatment, because loose bodies are generally secondary to other diseases that can cause persistent symptoms with the risk of new loose body formation. The aim of this narrative review is to show the clinical, imaging, and arthroscopic evaluation of loose bodies in order to plan optimal treatment. A comprehensive search of PubMed was conducted to find the most recent and relevant studies investigating aetiopathogenesis, the assessment tools, and the therapeutic strategies for loose bodies in the knee and their related diseases. When dealing with a loose body, the first issue is the evaluation of the intra-articular fragment (location, size, number, symptoms) and its aetiopathogenesis by identifying the underlying pathology (e.g., osteochondritis dissecans, osteoarthritis, chondral defect, tumour-like lesions, rheumatoid arthritis, etc.). In the case of symptomatic intra-articular loose bodies, treatment consists of fragment removal and the management of related diseases (e.g.., lifestyle modification, physiotherapy, pharmacological, and surgical treatment). Loose bodies are not separate entities and in addition to their pathological aspect, must be evaluated within the context of the underlying disease. Correct assessment and comprehensive management allow for relief of symptomatology and prevention of loose body formation by removal and treatment of the associated diseases.

Research advances of anesthesiology on neurosurgery in 2018

General topics in anesthesiology of neurosurgery include three aspects: anesthesiology for brain tumor patients, anesthesiology for cerebral vascular surgery and anesthesiology for traumatic brain injury. This review summarized literatures published in 2018 pertaining to the perioperative care of neurosurgical patients and patients with neurological diseases according to the three categories mentioned above. The topics include management of related disease in perioperative period, drugs used in perioperative period, methods of anesthesia and monitoring of perioperative period. Key words: Neurosurgery; Anesthesia; Brain tumor; Cerebral vascular surgery; Traumatic brain injury

Advances in liver transplantation for patients with primary sclerosing cholangitis

Primary sclerosing cholangitis (PSC) is a common autoimmune liver disease, and the diversity of its treatment methods has been hotly discussed by scholars in various countries. As the best treatment, end-stage liver transplantation (LT), due to the related diseases, such as postoperative complications greatly limits its wide application in clinical practice. Early assessment and intervention can reduce the risk of concomitant disease and reduce the occurrence of postoperative complications. In this paper, the status of LT indications, timing and related disease management, postoperative complications and other aspects of PSC patients were reviewed. Key words: Primary sclerosing cholangitis; Liver transplantation; Concomitant diseases; Postoperative complications

Transperitoneal Laparoscopic Approach to the Perinephric Area in Children: Technical Report and Lessons Learned.

This study presents a technical report of a standardized approach to the perinephric area in a series of pediatric patients, demonstrating that whatever renal or suprarenal surgery is planned, this can be approached and accomplished laparoscopically with an identical or very similar port triangulation, thus facilitating the learning curve within the same surgical team. All patients undergoing renal and adrenal gland surgery with a minimally invasive approach in the period from October 2008 to November 2013 were retrospectively reviewed and included in the study. Technical details and clinical outcomes are described. In total, 68 patients matched the inclusion criteria and were therefore retrospectively examined. No major intraoperative complication occurred. Two patients developed recurrent pelvic-ureteric junction obstruction and were managed with double J stent positioning and laparoscopic dismembered pyeloplasty, respectively. Two patients presenting with adrenal mass experienced a moderate bleeding that could be treated conservatively. Thanks to the reproducibility of the procedure and on the grounds of the presented experience, we support the proposal that the transperitoneal laparoscopic approach to the kidney and the adrenal gland could represent a relatively easy and safe way to quickly achieve confidence and skill in the management of related diseases.

Overall anatomical features and clinical value of the sacral nerve in high resolution computed tomography reconstruction

Sacral nerve injury is a common complication of pelvic or sacral fractures. As the sacral nerve courser within the sacrum and has a complex relationship with the surrounding tissues, different parts of the sacral plexus injury have similar clinical symptoms and signs. Since lack of specific imaging technique in the diagnosis of sacral nerve injury, especially on multi-segment, multi-site, how to determine the preoperative location and extent of the sacral nerve injury accurately becomes a concern of the general orthopaedic and images practitioners. This study was conducted to gain an insight into the overall anatomical features of the sacral nerve (SN) on the same slice in high resolution computed tomography (HRCT) reconstruction and to determine the value of this information for the clinical diagnosis of related diseases. Fifty healthy volunteers and 30 patients (40 sides) with SN lesions confirmed by surgery were scanned using a 16-slice helical CT scanner (Light Speed, GE, USA). Among the patients, 6 with intervertebral disk hernia (6 sides), 8 with spinal stenosis (12 sides), 11 with pelvic trauma (14 sides), 4 with pelvic malignancies (6 sides), and 1 with sacral vertebral tuberculosis (2 sides). The SN multiplanar reconstruction was performed using a UNIX-based SCD4.1 workstation where the image was set on the same slice. All images were stored in the Digital Imaging and Communications in Medicine format. The display of nerves in different sections was analyzed using a five-graded scale with coordinate curves of each individual score. The overall anatomic features visible on the slice were analyzed and the abnormalities of the lesions were studied. The image of the same slice clearly revealed the shape, running direction, thickness, tension and adjacent anatomy of the S1-S4 nerves. The rank of display rates in different sections was: outward-rotated oblique sagittal > outward-rotated oblique coronal > oblique coronal plane > coronal > sagittal > transverse section. The S5 nerve was partially displayed from the starting point to the segment around the posterior sacral foramen. The overall anatomy of the triangular sacral plexus was only revealed in the oblique outward-rotated sagittal section, while 100% of its individual rami, as well as two or three of the adjacent rami, were displayed from their starting points to the anterior border of the piriformis. The abnormalities included 39 sides of morphological change (97.5%), 38 sides of compression (95.0%), 35 sides of adhesion (87.5%), 32 sides of displacement (80.0%), 34 sides of shrinkage (85.0%), 6 sides of thickening (15.0%), and 2 sides of abruption (5.0%). The 16-slice CT multiplanar reconstruction was able to reveal the overall anatomic features of the SN on the same slice. The section of reconstruction was a crucial factor in determining the display capability of various sacral nerves. This technology was valuable in the diagnosis and management of related diseases.