Purpose: The purpose of this study is to derive implications for the prevention and management of musculoskeletal diseases of daily workers in the construction industry by comparing and analyzing the effects of job stress on musculoskeletal diseases among daily workers in the construction industry. Method: This study was conducted on 218 daily workers in the construction industry who participated in safety and health education in the construction industry among workers in the construction industry located in Daegu and Gyeongbuk. G*Power 3.1.9.7 analysis was used to find out the number of statistically significant research subjects, and 122 people were calculated and tested when a moderate effect size of 0.15, a significance level of 0.05, a power of 95% and two predictors were used for multiple regression analysis. Results: In the case of recognition of musculoskeletal disease, the age groups were significantly higher in their 50s and 60s, and in the single group by marital status. In the case of variations in perception of the severity of musculoskeletal disease symptoms according to socio-demographic characteristics, there were no variables showing significant variations, but job stress showed a certain relationship with musculoskeletal disease, and job stress had a significant positive effect on musculoskeletal disease morbidity. Conclusion: The results of this study suggest that measures to manage job stress should be sought to prevent musculoskeletal diseases and manage diseases of daily workers in the construction industry.