Background Symptomatic vaginal discharge is the most frequent symptom in women of reproductive age group. Discharge may be physiological or pathological, for which bacterial vaginosis is the most common cause, followed by candidiasis and trichomonas. Multiple infections can also coexist. Aim This study aimed to determine the microbiological and clinical profile of symptomatic vaginal discharge and its utility in the management of genital tract infection. Participants and methods This was a prospective study of 200 women with vaginal discharge referred for clinical examination and pelvic examination through a speculum examination according to the criteria of vaginal discharge. To determine the cause, high vaginal swab by Ayre’s spatula was obtained for the Microbiology Department through transport media. The collected data were tabulated and analyzed. Results Bacterial vaginosis is the most common cause of vaginal discharge, followed by candidiasis and trichomonas in our setup. For the etiological diagnosis of symptomatic vaginal discharge, the microbiological diagnostic approach is the best. A specific diagnosis was made in 83.5% of cases, whereas an etiology was not found in 16.5% of cases. Conclusion Treatment on the basis of a clinical diagnosis is not accurate. Thus, the most ideal approach in this study is the microbiological diagnostic approach for the etiological diagnosis of symptomatic vaginal discharge.