Males Of Different Ages Research Articles

Monitoring and Occurrence Prediction of the Migration Population of Helicoverpa armigera (Hübner) Based on Adult Semiochemical Attractants

Helicoverpa armigera (Hübner) is a destructive agricultural pest. Facultative migration usually causes regional catastrophes; therefore, developing a simple and easy new technology for the monitoring and early warning of immigrant populations is urgent. Between 2021 and 2023, we conducted a population-monitoring study on H. armigera immigrants in Xundian County, Yunnan Province, where the migration pathway for pests from southeast Asia extends to southwest China. Based on the differences in the reproductive organ development parameters of H. armigera at different ages, we established an adult age discrimination model. The monitoring results of field populations with semiochemical attractants and sex pheromones between 2021 and 2023 showed that the daily average age of the adult population of H. armigera fluctuated above 6 days, and the trapping dynamics with semiochemical attractants and sex pheromones were the same. Both trapping methods yielded H. armigera males of different ages and had identical age structures, indicating that the H. armigera population primarily migrated from other regions. The trajectory analysis showed that the H. armigera population that migrated to Xundian between May and September was primarily from South Kunming, and after October, it was primarily the southward-migrating population north of Qujing City, Yunnan Province. Based on the relationship between the daily average fecundity and the age of H. armigera, a dynamic prediction model for the fecundity of the immigrant population was established. In this study, the prediction models and methods based on semiochemical attractants could potentially be used in the surveillance and population alerting of H. armigera.

EVALUATION LITER SIZE OF DIFFERENT AGE MALES WITH DIFFERENT PARITY PARENTS ON SMALLHOLDER PIG FARMING IN BUAHAN VILLAGE-PAYANGAN


 
 
 This study aims to evaluate litter size of different ages males with different parent parity in smallholder pig far- ming, used 3 males of different ages (P1, P2, P3) and 18 sows of parity 1 – 4 (I1, I2, I3 and I4) with a 3 x 4 factorial RAL experimental design and unequal number of replications. The variables observed were litter size, weaning litter size, mortality and percentage of weaning litter size. Data were analyzed using a analyse of variant with the general linear model procedure. The results showed that the average litter size based on males P1, P2, P3 was 8.38; 9.13 and 10.38 (P>0.05) and based on parental parity I1, I2, I3 and I4 respectively 8.00; 8.83; 9.66 and 10.66 (P<0.05). For weaning litter size, respectively 5.75; 7.50 and 8.87 (P<0.05) and from parent parity was 6.17; 6.67; 8.17 and 8.50 (P<0.05), while the mortality respectively was 31.82%; 17.62% and 12.39% (P>0.05) and from parent parity respec- tively 23.54%; 23.21%; 15.74%; and 19.94% (P>0.05). Weaning litter size percentage of each male was 68.17; 82.50; 87.61 (P>0.05) and from parental parity 76.63; 76.78; 84.26 and 80.05 (P>0.05). Older males and fourth parity of parent produced better litter size, weaning litter size and weaning litter percentage with lower mortality. There was no interaction between the use of males of different ages and parents of different parity on the observed variables.
 
 

Acute exercise-induced release of innate immune proteins via small extracellular vesicles changes with aerobic fitness and age.

Physical exercise triggers the secretion of small extracellular vesicles (sEVs) into the circulation in humans, enabling signalling crosstalk between tissues. Exercise-derived EVs and their cargo have been proposed to mediate adaptations to exercise; however, our understanding of how exercise-derived EV protein cargo is modulated by factors such as aerobic fitness and age of an individual is currently unknown. Here, we examined the circulating sEV proteome following aerobic exercise in healthy males of different ages and aerobic fitness to understand exercise-induced EV response during the aging process. Twenty-eight healthy men completed a bout of 20-min cycling exercise at 70% estimated VO2peak . Small EVs were isolated from blood samples collected before and immediately after exercise, and then quantified using particle analysis and Western blotting. Small EV proteome was examined using quantitative proteomic analysis. We identified a significant increase in 13 proteins in small plasma EVs following moderate-to-vigorous intensity exercise. We observed distinct changes in sEV proteome after exercise in young, mature, unfit, and fit individuals, highlighting the impact of aerobic fitness and age on sEV protein secretion. Functional enrichment and pathway analysis identified that the majority of the significantly altered sEV proteins are associated with the innate immune system, including proteins known to be damage-associated molecular patterns (DAMPs). Together, our findings suggest that exercise-evoked acute stress can positively challenge the innate immune system through the release of signalling molecules such as DAMPs in sEVs, proposing a novel EV-based mechanism for moderate-to-vigorous intensity exercise in immune surveillance pathways.

Morphological characterization of the Polatli sheep in terms of live weight using data mining algorithms.

The aim of this research is both to estimate the live weight (LW) of Polatli sheep (Ile de France × Akkaraman (G1)) by considering some body measurements (withers height (WH), rump height (RH), body length (BL), chest depth (CD), chest width (CD), chest girth (CG), cannon bone circumference (CBC)), age, and sex variables as independent variables using C&RT (Classification and Regression Tree), CHAID (Chi-square Automatic Interaction Detector), and MARS (Multivariate Adaptive Regression Splines) algorithms and to determine the significant independent variables in the estimation of live weight. For this purpose, a total of 210 sheep were used, including 180 females and 30 males of different ages, for the estimation of LW. The calculated Pearson correlation coefficients between LW and WH, RH, BL, CD, CW, CG, and CBC characteristics are 0.897, 0.896, 0.853, 0.948, 0.550, 0.914, and 0.798, respectively (p < 0.05). In the application of data mining algorithms as prediction models, a cross-validation of 10 was used, while for tree-based algorithms, the parent node was set to 10 and the child node to 5. While CHAID and C&RT algorithms each used 8 independent variables to explain the variation observed in LW, the MARS algorithm used 9 independent variables. In Polatli sheep, the sheep with the highest live weight was found in the node with age > 3 and CD > 36 cm cutting point in the CHAID algorithm (93.571 kg). In the C&RT algorithm, it was predicted to be (91.316 kg) when age > 0, CD > 36.5 cm, and CBC > 9.5 cm. When evaluated considering commonly used criteria, the prediction performances of CHAID, C&RT, and MARS algorithms were calculated as follows: RMSE (root mean square error) values are "5.788, 5.103, 4.005"; SDR (standard deviation ratio) values are "0.254, 0.224, 0.176"; MAPE (mean absolute percentage error) values are "7.555, 6.675, 5.682"; Adj-Rsq (adjusted R-squared) values are "0.935, 0.950, 0.969"; and AIC (Akaike information criterion) values are "741.436, 688.489, 582.792," respectively. In terms of prediction performance, among the tree algorithms (CHAID and C&RT), C&RT was found to be the best, while considering all performance measures, it was observed that the MARS algorithm exhibited the best performance. Consequently, it has been determined that C&RT and MARS algorithms can be safely employed in morphological characterization studies for the identification of indirect criteria and the formation of elite herds in terms of LW. This decision allows for the reliable use of these algorithms to facilitate the selection of indirect variables and the establishment of elite populations in breeding programs focusing on live weight.

Age, Sperm Retrieval, and Testicular Histology in Klinefelter Syndrome.

We sought to examine sperm retrieval and testicular histology in males of different ages with Klinefelter syndrome. We identified all males with Klinefelter syndrome who underwent microdissection testicular sperm extraction at our institution from 1995 to 2020. Patients were divided into adolescent (<20 years) and adult (≥20 years) cohorts. Histology and sperm retrieval were compared using chi-square statistics. Multivariable logistic regression models were used to examine factors associated with successful sperm retrieval. We identified 217 males with Klinefelter syndrome, of whom 59 were adolescents and 158 were adults. Adults were stratified into 10-year groupings (20-29 years, n = 62; 30-39 years, n = 88; ≥40 years, n = 8). Approximately 17% of adolescents had testis histology containing germ cells compared with 15% of the 20 to 29-year cohort, 14% of the 30 to 39-year cohort, and 0% over 40 years. In comparison to adolescents (53%), the sperm retrieval rate was significantly higher in the 20 to 29-year cohort (71%, P = .04) and lower in the ≥40-year cohort (13%, P = .03). In multivariable analysis, the presence of hypospermatogenesis on testis biopsy (OR 5.8, P = .03) was associated with higher odds of successful sperm retrieval. Younger males more frequently had germ cell-containing testis histology, however this finding was not associated with a higher odds of sperm retrieval. Reproductive urologists should counsel azoospermic males with Klinefelter syndrome that sperm retrieval during adolescence for fertility preservation is not required and can be performed in young adulthood.

The effect of hypomagnetic field on survival and mitochondrial functionality of active Paramacrobiotus experimentalis females and males of different age.

Even for tardigrades, often called the toughest animals on Earth, a hypomagnetic field (HMF) is an extreme environment. However, studies on the effect of HMF on tardigrades and other invertebrates are scarce. Mitochondria play an important role in an organism's response to extreme conditions. The effect of HMF on the mitochondrial inner membrane potential (Δψ), a well-known marker of mitochondria functionality, shows that mitochondria are very sensitive to HMF. To measure the HMF effect on Paramacrobiotus experimentalis, we calculated the tardigrade survival rate and Δψ level after HMF treatments of different durations. We also estimated the relationship between the age and sex of the tardigrade and the HMF effect. We observed age- and sex-related differences in Δψ and found that Δψ changes after HMF treatment were dependent on its duration as well as the animal's age and sex. Furthermore, active P. experimentalis individuals displayed a high survival rate after HMF treatment. The data may contribute to the understanding of tardigrade aging and their resistance to extreme conditions including HMF, which in turn may be useful for future space explorations.

Use of Food Attractant to Monitor and Forecast Population Dynamics of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae), a Long-Distance Migratory Pest

The rice leaf folder, Cnaphalocrocis medinalis, is a significant migratory pest that restricts rice production in Asia and Africa, and monitoring and early warning measures are the basis for its management. Based on its regional migratory path in China, the pest population dynamics were monitored from 2020 to 2021 using food attractants. In this study, we also used internal reproductive system dissection to determine the adult age and reproductive developmental state. The monitoring results indicated that the trapping dynamics of C. medinalis males using food attractants and sex pheromones were approximately similar. Both trapping methods yielded C. medinalis males of different ages, with an identical age structure for both trap types. Dissection analysis of the reproductive system of females trapped using the food attractants indicated that in 2020, the population of C. medinalis in Liling, Hunan Province, was indigenous, while populations in other monitoring sites mainly comprised migrants from other areas. The ovarian development level of females trapped using food attractants showed a positive correlation with the age of males, and there was also a significant positive correlation between the index of ovarian development of females and the mating time. Furthermore, a dynamic prediction method for fecundity in field populations was established based on the quantity of eggs held by the females. This study provides a new method for the monitoring and early warning of the existence of C. medinalis populations.

A novel epilepsy seizure prediction model using deep learning and classification

Epilepsy is a common neurological disease where the earlier disease prediction significantly impacts those patients’ lives. In this paper, a novel epilepsy seizure prediction approach is designed using deep learning. The proposed model is applied to the Electroencephalogram (EEG) recordings collected from Children’s Hospital Boston (CHB-MIT). The recording data is grouped into 23 cases, including 17 females and five males of different ages. The recordings are sampled at 256 samples/s of 16-bit resolution. The target is to analyse the brain’s state and evaluate the changes encountered from the interictal state. The earlier prediction process helped in timely disease identification and treatment to rescue the patients. Feeding the raw EEG signals over the feature extractor reduces the computational complexity and execution time. An Adaptive Grey Wolf Optimizer (AGWO) is used for learning the features and promoting those discriminative features to enhance the prediction rate and classification accuracy. To optimize the features integrating the auto-encoder concept with Genetic Algorithm (GA) in an adaptive manner termed as to enhance the prediction rate. The functionality of is tested over the subjects of the CHB-MIT EEG dataset to achieve resourceful outcomes. The proposed attains higher accuracy of 99% and reduces the False Alarm Rate (FAR) with little prediction time. The model’s functionality is evaluated using the MATLAB simulation environment and shows a better trade-off than existing approaches.

MOLECULAR STUDY TO SOME FUNGI ISOLATED FROM PHONE COVER AND IT'S USERS

60 samples were randomly isolated 30 samples from the cover of a mobile phone and 30 samples from the finger of its users for people from the city of Mosul - Iraq, most of them are pioneers of life sciences, College of Science, University of Mosul, with a ratio of 15 females and 15 males of different ages, occupation, region, type of device, number of hours of use, and the last date taken. Washing the hands and the user's hand, right or left. The results showed that the cover of the mobile device of a female student in the Department of Life Sciences, College of Science, University of Mosul, at the age of 27 years, was contaminated with the fungus Chaetomium globosum, which grew on PDA medium.it was also noticed that the finger of the mobile device was contaminated by a female student in the Department of life Sciences College of sciences, University of Mosul,28 years old with The yeast Meyerozyma caribbica, and these two samples were recorded in the Gen Bank of the National Center for Biotechnology Information NCBI, the fungus was recorded as Chaetomium globosum B-M1 and given an identification number LC723824.1, while the yeast Meyerozyma caribbica was recorded as Meyerozyma caribbica B-M2 and with definition number is LC723825, .By studying the genetic distance and affinity tree of the fungus Chaetomium globosum B-M1 it was observed that it was identical (2.283), while the affinity ratio with the Chinese one was (2.582) and it was genetically far from the Iranian isolate by (2.852). As for the American isolate, there was genetic divergence with the Iraqi local isolate by (3326.), while it was found from the tree of distance and genetic affinity for the yeast Meyerozyma caribbica B-M2 that it was identical by ( 0.001) with its Japanese counterpart and was genetically far from the Australian isolate by (2.086) . As for the Italian and Dutch isolates there was a genetic divergence with the Iraqi local isolate by (2.304).

Male larval experience of cues from adult rivals alters lifetime sperm investment patterns in a sperm heteromorphic moth, Ephestia kuehniella.

Male animals may adjust their resource allocations for reproduction and other fitness functions in response to cues from rivals. For instance, adult males increase their investment in sperm for a higher paternity share when they perceive sperm competition risk in their surroundings. In nature, both juveniles and adults may coexist spatially and temporally. Yet, it is not clear how juvenile males of different ages respond to cues from adult rivals and fine-tune their lifetime investment in sperm production and ejaculation in any insect. Here we used the Mediterranean flour moth, Ephestia kuehniella, which produces both fertile eupyrene and infertile apyrene sperm, to explore this question. We demonstrate that the late, but not early, instar larvae are sensitive to adult male cues. As a response, they produce more sperm before emergence and their resultant adults have shorter mating latency and ejaculate more sperm in the first few matings. When the juvenile stage produces more eupyrenes, the adult stops making these sperm, but regardless of the number of apyrenes produced during the juvenile stage, the adult continues to make them. These findings suggest that the number of spermatogonia for eupyrenes may be limited and that for apyrenes may be flexible. Our results show that the insect does not trade off survival, mating frequency, body size, or testis size for sperm production in response to adult males during the larval stage. Knowledge created in the present study offers insight into the stage-dependent sensitivity of juvenile males to cues from adult rivals and subsequent lifetime resource allocations.

Novel Insights from Fourier-Transform InfraRed Imaging on the Morpho-Chemical Profile of Human Corpus Callosum

The corpus callosum (CC) is the largest interhemispheric commissure of the mammalian brain, and it includes axons, cortical neurons, and glial cells. It is mainly composed of myelin, a lipidic sheath which is produced by glial cell membranes; myelin is wrapped up around axons and plays a fundamental role in the fast conduction of neuronal electrical signals. The human CC is divided into various anatomical regions, with different axonal composition, including, from front to back, genu, body or trunk, isthmus, and splenium. Corpus callosum undergoes some alterations not only in the presence of specific physiological and pathological conditions, but also because of aging. For the first time, in the present study a hyperspectral imaging analysis of human corpus callosum was performed. The study, carried out on CC autopsy samples collected from human adult males of different ages, was focused mainly on the genu and splenium regions. By combining Fourier-transform infrared imaging and histological analyses with multivariate and univariate ones, the macromolecular composition of these regions was defined, and age-related alterations in the lipid and protein components were identified.

Correlation between atherogenic index of plasma and coronary artery disease in males of different ages: a retrospective study

BackgroundAtherogenic index of plasma (AIP) as a newly discovered blood lipid parameter was shown to be strongly correlated with coronary artery disease (CAD). However, the blood lipid levels changed with age, so we speculated that the correlation between AIP and CAD was also affected by age.MethodsA retrospective study was performed on male patients with confirmed or suspected CAD who underwent coronary angiography (CAG) from July 2017 to March 2021. Patients were divided into younger, middle early, middle late, and elderly groups. Patients in each age group were further divided into the non-coronary artery disease (n-CAD) and CAD subgroups according to the CAG diagnostic results. The relationship between blood lipid parameters and CAD was assessed in each age group.ResultsAge-dependent changes of blood lipid levels were mainly found in CAD patients but not in n-CAD patients. With increased age, the levels of triglyceride, total cholesterol, low-density lipoprotein cholesterol (LDL-C) and AIP were gradually decreased, whereas high-density lipoprotein cholesterol concentration was increased in CAD patients. Multivariate logistic regression analyses showed that AIP was an independent risk factor for CAD in middle early (OR 2.601; CI 1.160–5.832, P = 0.02) and middle late age group (OR 2.707, CI 1.201–6.100, P = 0.016), but not in the younger and elderly groups. LDL-C was an independent risk factor for CAD in all age groups. The areas under curve of AIP for detecting CAD in the middle early and middle late age groups were not higher than that of other blood lipid parameters.ConclusionAlthough this was a single-center study for males only, the correlation between AIP level and CAD risk was age-dependent. AIP was an independent risk factor for CAD in the middle-aged groups. However, the predictive value of AIP for detecting CAD was not better than that of the traditional blood lipid parameters such as LDL-C.

PROTOTYPING SMART HOME FOR IMMOBILIZED PEOPLE: EEG/MQTT-BASED BRAIN-TO-THING COMMUNICATION

Context. Immobilized people face additional barriers in almost all areas of life, including simple operations like turning the light on/off and controlling the air conditioner. The object of the study was to develop the brain-to-thin communication of affordable priceto control the smart home appliances by immobilized people from neck to toes. Objective. The goal of the work is to manage smart home appliances via brain-to-thing communication with EEG non-invasive electrodes, edge IoT devices, and MQTT protocol if the brain and eye control of the disabled work normally. Method. A non-invasive Sichiray TGAM brainwave EEG sensor kit captures signals and then transmit them via Bluetooth to the HC-05 module connected to the Arduino Mega microcontroller. Information about edge IoT devices is presented to the disabled on the LCD 1602 display wired to the same Arduino Mega. The disabled person chooses the option shown on display via the double blink that is detected if the quality of signal equals zero and low/mid gamma waves are less than ten in three consecutive Bluetooth packets. Control commands are sent from Arduino Mega (MQTT publisher) to the edge IoT devices (MQTT subscribers) that analyze them and start a specific operation like opening a door and turning the alarm on/off. Results. Five females and five males of different ages from 8 to 59 years old examined the control of smart home appliances with the Sichiray TGAM brainwave sensor kit. Everyone successfully handled the Sichiray headset and showed satisfaction with the brain-to-thing system. Conclusions. In this work, a smart home concept for immobilized people was developed using the brain-to-thing approach and the MQTT communication between the MQTT publisher, Sichiray TGAM brainwave EEG sensor kit connected via Bluetooth to the Arduino Mega microcontroller, and edge IoT devices total priced at USD 150. The most likely prospect of the presented work is to produce the sample that is ready to market.

Oral D-Aspartate Treatment Improves Sperm Fertility in Both Young and Adult B6N Mice

Simple SummaryInvestigations concerning the impact of D-Aspartate on fertility suggest that it has a positive influence on the in vitro fertilization rate in young C57BL/6N mice. Here, we demonstrated that adult C57BL/6N mice that received an oral treatment of D-Aspartate also have a higher fertilizing capability and the quality of their spermatozoa increased after only two weeks of treatment. Hence, this study gives us new insights on the role of D-Aspartate in the regulation of the reproductive activity in both young and adult mice.D-Aspartate (D-Asp) treatment improved the fertility of young male C57BL/6N mice in vivo revealing a direct role on capacitation, acrosome reaction, and fertility in vitro in young males only. We investigated whether the positive effect of D-Asp on fertility could be extended to adult males and evaluated the efficacy of a 2- or 4-week-treatment in vivo. Therefore, 20 mM sodium D-Asp was supplied in drinking water to males of different ages so that they were 9 or 16 weeks old at the end of the experiments. After sperm freezing, the in vitro fertilization (IVF) rate, the birth rate, hormone levels (luteinizing hormone (LH), epitestosterone, and testosterone), the sperm quality (morphology, abnormalities, motility, and velocity), the capacitation rate, and the acrosome reaction were investigated. Oral D-Asp treatment improves the fertilizing capability in mice regardless of the age of the animals. Importantly, a short D-Asp treatment of 2 weeks in young males elevates sperm parameters to the levels of untreated adult animals. In vivo, D-Asp treatment highly improves sperm quality but not sperm concentration. Therefore, D-Asp plays a beneficial role in mouse male fertility and may be highly relevant for cryorepositories to improve mouse sperm biobanking.

Sex and age differences in migraine treatment and management strategies.

Migraine is one of the leading causes of disability worldwide, especially in women younger than 50 years old. Migraine has three times higher prevalence in women than in men and tends to decrease after the menopausal transition. Migraine has different clinical features in people of different ages. Clinical symptoms and factors associated with migraine can be various in women and men. Women have special types of migraine, such as pure menstrual migraine and menstrually related migraine. Besides, clinical symptoms of migraine can change during pregnancy, postpartum and lactation. Women are significantly more often than men consulting a doctor because of migraine. These features of migraine lead to different treatment and management strategies in females and males of different ages. Migraine therefore is a disorder that demonstrates the necessity of a personalization of healthcare-ensuring the proper treatment for the right patient, at the right time. Considering all the available literature and guidelines, in this chapter several strategies for management of acute and prophylactic treatments of migraine, according to sex and age differences, are discussed. The purpose of this chapter is to provide a useful piece of information improving the treatment and management of migraine.

The Re-Addition of Seminal Plasma after Thawing Does Not Improve Buck Sperm Quality Parameters.

Simple SummaryThe Cabra Blanca de Rasquera is a native goat breed from Catalonia (Spain) facing the danger of extinction. To preserve its genetic diversity, ex situ conservation programs for semen cryopreservation and the development of a genetic sperm bank are of major importance. However, the post-thaw sperm quality in this species is still a major concern. Therefore, the aim of this research work was to establish an approach in the thawing protocol of cryopreserved sperm bank doses of this breed in order to achieve a better post-thaw sperm quality and improve reproductive outcomes through artificial insemination. Since seminal plasma has been found to provide a source of nutrients, improve sperm motility, and prevent premature activation during migration of spermatozoa in the female reproductive tract, our study proposed to assess the effect of the addition of 20% seminal plasma (SP) during a 3 h incubation period under in vitro conditions mimicking the in vivo survivability of thawed sperm, in relation to donor age, the season of collection, and melatonin implants of the males in the non-breeding season. However, the strategy of adding seminal plasma to the sperm after thawing failed to improve buck post-thawed sperm quality, thus further investigation is needed.In order to achieve a higher post-thaw buck sperm quality, an approach in the thawing protocol of cryopreserved sperm doses under in vitro capacitation conditions mimicking the in vivo female environment was studied. Therefore, functional and kinetic characteristics of buck thawed sperm from males of different ages, the season of collection, and melatonin implanted males in the non-breeding season were assessed after 3 h of incubation in an in vitro fertilization (IVF) media with 20% of buck seminal plasma (SP). Previously, fresh ejaculates were collected via artificial vagina from eight males of the Cabra Blanca de Rasquera breed during two consecutive years in breeding and non-breeding periods. Prior to semen collection in non-breeding seasons, males were split into two groups: one group was implanted with melatonin, while the other was not. In each group, semen samples were pooled, centrifuged, and diluted in an extender containing 15% powdered egg yolk and 5% glycerol before freezing. After thawing, sperm were washed and incubated in three different media: (a) control media (modified phosphate-buffered saline (PBS), (b) IVF commercial media, and (c) IVF media + 20% SP. Sperm motility was evaluated by CASA, while plasma and acrosome membrane integrity, mitochondria activity, and DNA fragmentation were analysed by flow cytometer at 0 h and after 3 h incubation. A significant reduction in motility, mitochondrial activity, plasma, and acrosome membrane integrity were observed after incubation in the presence of SP, although similar to that observed in IVF media alone. DNA integrity was not affected under in vitro capacitation conditions, regardless of SP addition. In conclusion, the addition of SP failed to improve post-thaw buck sperm quality under in vitro conditions irrespective of male age, the season of collection, and melatonin implant.

Ability of dog owners to identify their dogs by smell

Several studies report that olfactory cues play an important role in human life; humans are essentially able to recognize other family members and friends by their odors. Moreover, recent studies report that humans are also able to identify odors of non-conspecifics. The aim of this study was to determine whether dog owners are able to identify their dogs by smell and distinguish the odor of their own dogs from those of other dogs. A total of 53 dog owners (40 females and 13 males of different ages) volunteered to take part in this study. A number of the participants (17) owned 2 dogs; these owners took part in the study twice (i.e., working with only one dog at a time). Sterile gauze pads were used to collect odor samples from the dogs. Each pad was placed in its own sterile glass jar (750 ml) with a twist off lid until the experiment commenced. Participants were asked to identify their own dog´s odor from a line-up of 6 glass containers. This experiment demonstrated that dog owners are capable of identifying their dogs by smell on a significant level. Results of this study additionally suggested that male owners outperformed their female counterparts in the identification process. Moreover, dog owners whose dogs were housed outside had a higher success rate in identification than did participants who kept their dogs indoors with them. The dog owners found it easier to identify dogs that had been neutered, fed dry dog food and bathed less frequently. In general, younger dog owners tended to have more success when attempting to identify their dogs than did their older counterparts.

Integrated Sensing Systems for Monitoring Interrelated Physiological Parameters in Young and Aged Adults

Acute injury to aged individuals represents a significant challenge to the global healthcare community as these injuries are frequently treated in a reactive method due to the infeasibility of frequent visits to the hospital for biometric monitoring. However, there is potential to prevent a large number of these cases through passive, at-home monitoring of multiple physiological parameters related to various causes that are common to aged adults in general. This research strives to implement wearable devices, ambient “smart home” devices, and minimally invasive blood and urine analysis to test the feasibility of implementation of a multitude of research-level (i.e. not yet clinically validated) methods simultaneously in a “smart system”. The system comprises measures of balance, breathing, heart rate, metabolic rate, joint flexibility, hydration, and physical performance functions in addition to lab testing related to biological aging and mechanical cell strength. A proof-of-concept test is illustrated for two adult males of different ages: a 22-year-old and a 73-year-old matched in body mass index (BMI). The integrated system is test in this work, a pilot study, demonstrating functionality and age-related clinical relevance. The two subjects had physiological measurements taken in several settings during the pilot study: seated, biking, and lying down. Balance measurements indicated changes in sway area of 45.45% and 25.44%, respectively for before/after biking. The 22-year-old and the 73-year-old saw heart rate variabilities of 0.11 and 0.02 seconds at resting conditions, and metabolic rate changes of 277.38% and 222.23%, respectively, in comparison between the biking and seated conditions. A smart camera was used to assess biking speed and the 22- and 73-year-old subjects biked at 60 rpm and 28.5 rpm, respectively. The 22-year-old subject saw a 7 times greater electrical resistance change using a joint flexibility sensor inside of their index finger in comparison with the 73-year-old male. The 22 and 73-year-old males saw respective 28% and 48% increases in their urine ammonium concentration before/after the experiment. The average lengths of the telomere DNA from the two subjects were measured to be 12.1 kb (22-year-old) and 6.9 kb (73-year-old), consistent with their biological ages. The study probed feasibility of 1) multi-metric assessment under free living conditions, and 2) tracking of the various metrics over time.

Effects of age on sperm quality metrics in endangered Mississippi gopher frogs (Lithobates sevosus) from captive populations used for controlled propagation and reintroduction efforts.

A decline in sperm quality with age is a common prediction of senescence-based hypotheses and empirical studies. While widely studied across taxa, there is little known on the effect of ageing on sperm quality in amphibians, especially in captive populations used for controlled propagation and reintroduction efforts. Here, we investigated variation in sperm quality metrics (i.e., motility, concentration, and morphology) in the endangered Mississippi gopher frog (Lithobates sevosus) among males of three age categories using individuals from captive breeding populations housed at three different zoological institutions. Different aged males across the species expectant lifespan (1-9, 1-2, 3-4, and 8-9-year-old subcategories) were chosen in an attempt to identify an optimal breeding age relevant for captive breeding programs. Moreover, we explored and statistically controlled for potential differences in sperm quality which may be attributed to the type of induction hormones and source populations that differed among institutions. Results indicated that males of different ages did not differ in sperm motility or concentration. However, we did find that older males (8-9 years old) had significantly longer sperm than other age categories and younger males (1-2 years old) had significantly more atypical sperm than other age categories. Furthermore, we found no significant differences in any sperm quality metrics between the different induction hormones or source populations used at the different institutions. Within a captive breeding program, this information is especially valuable as our results indicate that males that have only recently sexually matured may not be ready to breed, while older males maintain sperm quality metrics presumably related to fertilization success.

Importance of Dehydroepiandrosterone Sulfate Assessment with Special Attention for Adrenal Tumours and Arterial Hypertension.

To investigate the significance of DHEAS assessment in males of different ages. Retrospective cohort study of patients investigated in two large academic centres. The data of DHEAS assessment of 3533 patients (3013 females and 520 males) was analysed. DHEAS was 1.6 - 13.5 times more frequently investigated in women than in men. A peak of DHEAS evaluation test for women was at 25 years old and distribution was uniform in males over decades, excepting being lower in 0-9 and 75+ages. In the age group 10-24 years, DHEAS levels were higher in females. After 45 years, DHEAS was higher in men than in women. Analysis of 510 case records showed low DHEAS levels in boys (0-9 years) and in men aged 65 - 84+. Higher DHEAS levels were detected as a peak at 30 years old, but never after 55 years. In individuals with low DHEAS levels prevailed congenital adrenal hyperplasia (32%), adrenal tumours (30%) and primary or secondary adrenal insufficiency (19%). High DHEAS levels prevailed in patients with arterial hypertension (26%), overweight-obesity -(19%), non-toxic goiter (17%) and alopecia (9%). In the normal DHEAS miscellaneous diagnoses were met most frequently - 40%. Disorders exceeding 5% were non-toxic goiter (19%), adrenal tumours - 17%, overweight/obesity - 16% and arterial hypertension- 8%. In 71 women and 124 men adrenal neoplasms were detected. Higher frequency of these was observed in women in their 30s. A peak of adrenal neoplasms in men was at their 70s. This gender difference was not conditioned by earlier attempts to seek medical care by women. A significant correlation of DHEAS, weight, body mass index and systolic blood pressure with diastolic blood pressure was found. Our study permits to determine which DHEAS secretion and clinical pattern might be associated in males of different ages.