Male Silkworm Research Articles

Physicochemical Characterization of Oil and Chitosan Extracted from Cross Breed and Bivoltine Hybrid Silkworm Pupae: A Comparative Analysis

The oil and chitosan were extracted from silkworm pupae and analysed its physicochemical properties. Oil content in silkworm pupae ranged from 26 to 28 % on a dry weight basis. Notably, female pupae had a higher oil content (27.99 %) compared to males (27.03%). Further, bivoltine hybrids exhibited a slightly higher oil content (27.65 %) than cross breeds (27.30 %). In comparison to edible oils like groundnut oil and sunflower oil, the pupal oil showed favourable results in terms of moisture content, iodine value and saponification values, indicating quality similar to edible oils. However, acid value of silkworm pupal oil (1.570 mg KOH/g oil) was lower than that of groundnut oil (3.125 mg KOH/g oil). The acid value and peroxide value were found to be lower than three in pupal oil samples, revealed that pupal oil is good for edible purposes and indicates good oxidative stability. Among the samples, Chitosan content was higher 2.526% in the cross breed (PM × CSR2) than bivoltine hybrid 2.308%. Male silkworm pupae had a higher chitin content (3.242%) compared to female pupae (3.013%) and chitosan content was also higher (2.430%) in male pupae than in female pupae (2.345%). Pupal chitosan shows better solubility (99 %), degree of deacetylation (>85%) and ash content (<1%). These physicochemical properties were achieved the desired level for utilizing the silkworm pupal oil and chitosan as commercial utilization.

The Impact of Diabetes on Male Silkworm Reproductive Health.

The increasing prevalence of diabetic reproductive complications has prompted the development of innovative animal models. The use of the silkworm Bombyx mori as a model for diabetic reproductive damage shows potential as a valuable research tool. This study employed silkworms as a novel model to investigate diabetic reproductive damage. The silkworms were fed a high-glucose diet containing 10% glucose to induce a diabetic model. Subsequently, the study concentrated on assessing the influence of diabetes on the reproductive system of male silkworms. The results indicate that diabetes resulted in reduced luteinizing hormone (LH) and testosterone (T) levels, as well as elevated triglyceride (TG) levels in male silkworms. Moreover, diabetes mellitus was associated with pathological testicular damage in male silkworms, accompanied by decreased glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) levels, along with increased malondialdehyde (MDA) levels in the testis. Additionally, diabetes mellitus reduced the expression of siwi1 and siwi2 genes in the testis of male silkworms. Overall, these results support using silkworms as a valuable model for studying diabetic reproductive damage.

Comparison of lipid profiles of male and female silkworm (Bombyx mori) pupae through high-resolution mass spectrometry-based lipidomics and chemometrics

Silkworm (Bombyx mori) pupae lipid profiles were analyzed using high-resolution mass spectrometry-based lipidomics. A total of 241 lipid molecular species were annotated with high confidence in male and female silkworm pupae. Triacylglycerol (TG), phosphoethanolamine (PE) and phosphocholine (PC) were the main lipid subclasses of silkworm pupae, accounting for 63, 41 and 38 lipid molecular species, respectively. No unique lipid molecular species were identified, but there were differences in the abundance of lipid molecular species between male and female silkworm pupae. Therefore, the differences in the lipid abundance of male and female silkworm pupae were analyzed by chemometrics. As a result, 8 lipid molecular species were screened for differential lipids. Hierarchical clustering analysis (HCA) showed that male and female silkworm pupae samples formed two distinct branches, indicating that these differential lipids could potentially be used as biomarkers to distinguish between male and female silkworm pupae.

The Role of BmTMED6 in Female Reproduction in Silkworm, Bombyx mori.

Transmembrane emp24 domain (TMED) proteins have been extensively studied in mammalian embryonic development, immune regulation, and signal transduction. However, their role in insects, apart from Drosophila melanogaster, remains largely unexplored. Our previous study demonstrated the abundant expression of BmTMED6 across all stages and tissues of the silkworm. In this study, we investigate the function of BmTMED6 in reproduction. We observe significant differences in the expression of BmTMED6 between male and female silkworms, particularly in the head and fatboby, during the larval stage. Furthermore, qRT-PCR and WB analysis reveal substantial variation in BmTMED6 levels in the ovaries during pupal development, suggesting a potential association with silkworm female reproduction. We find that reducing TMED6 expression significantly decreases the number of eggs laid by female moths, leading to an accumulation of unlaid eggs in the abdomen. Moreover, downregulation of BmTMED6 leads to a decrease in the expression of BmDop2R1 and BmDop2R2, while overexpression of BmTMED6 in vitro has the opposite effect. These indicate that BmTMED6 plays a role in oviposition in female moths, potentially through the dopamine signaling pathway. This study provides a new regulatory mechanism for female reproduction in insects.

Metabonomic Analysis of Silkworm Midgut Reveals Differences between the Physiological Effects of an Artificial and Mulberry Leaf Diet.

Bombyx mori is a model lepidopteran insect of great economic value. Mulberry leaves are its only natural food source. The development of artificial diets can not only resolve the seasonal shortage of mulberry leaves but also enable changes to be made to the feed composition according to need. Metabolomic differences between the midguts of male and female silkworms fed either on fresh mulberry leaves or an artificial diet were studied using liquid chromatography-mass spectrography (LC-MS/MS) analysis. A total of 758 differential metabolites were identified. Our analysis showed that they were mainly involved in disease resistance and immunity, silk quality, and silkworm growth and development. These experimental results provide insights into the formulation of optimized artificial feed for silkworms.

Juvenile hormone suppresses the FoxO-takeout axis to shorten longevity in male silkworm

Juvenile hormone (JH) plays a crucial endocrine regulatory role in insect metamorphosis, reproduction, and longevity in multiple organisms, such as flies, honeybees, and migratory monarch butterflies. However, the molecular mechanism of JH affecting longevity remains largely unknown. In this study, we showed that JH III and its analog methoprene shortened the survival days significantly in the adulthood of male silkworm. At the same time, the allatostatin, a neuropeptide that inhibits the secretion of JH by the corpora allata, could extend the survival days dramatically after adult eclosion in male silkmoth. Interestingly, a central pro-longevity FoxO transcription factor was reduced upon JH stimulation in silkworm individuals and BmN-SWU1 cells. Furthermore, the analysis of the upstream sequence of the FoxO gene identified a JH response element which suggested that FoxO might be regulated as a target of JH. Surprisingly, we identified a Bmtakeout (BmTO) gene that encodes a JH-binding protein and contains a FoxO response element. As expected, FoxO overexpression and knockdown up- and down-regulated the expression of BmTO respectively, indicating that BmTO functions as a FoxO target. BmTO overexpression could release the inhibitory effect of JH on the BmFoxO gene by reducing JH bioavailability to block its signal transduction. Collectively, these results may provide insights into the mechanism of the JH-FoxO-TO axis in aging research and pest control.

Extraction and quantification of 1-Deoxynojirimycin in Silkworm during metamorphosis and moulting stages

The contents of 1-deoxynojirimycin (1-DNJ) in the silkworm, Bombyx mori, were examined using reverse-phase high-performance liquid chromatography at various developmental stages. Mulberry leaves and silkworms naturally contain 1-deoxynojirimycin (DNJ). Although the contents of DNJ and its actions in mulberry leaves have been studied for decades, the content of DNJ in silkworms and its use have received less attention. DNJ was extracted and quantified in a variety of silkworms including Tasar silkworms, Muga silkworms, Eri silkworms and Mulberry silkworms. Group VI silkworms are more active than the others. Then, for male and female larvae of silkworm species, the variation in DNJ contents was examined. Silkworm larvae's 1-DNJ concentration varies greatly depending on their developmental stage. Male larvae (133.25 ± 11.45 mg/100g) and female larvae (129.65 ± 10.25 mg/100g) are substantially higher in both male and female mulberry silkworms. The larvae of the 3rd and the 5th day were used to calculate the DNJ. Male larvae had a higher 1-DNJ accumulation efficiency than females and there was also a significant difference between silkworm strains. The current findings demonstrate that 1-DNJ tissue distribution is substantially increased in the blood, intestinal juice and the alimentary canal while none was found in the silk gland. Furthermore, 1-DNJ was not detected in silkworms fed with an artificial diet devoid of mulberry leaf powder. This shows that silkworms get 1-DNJ from mulberry leaves; they could not make it on their own. During the larval stage, the storage and excretion of 1-DNJ fluctuate. 1-DNJ was not found in newly hatched larvae and it was primarily accumulated during the early and middle stages of each instar, before being expelled at later stages. Furthermore, 1-DNJ can be extracted from larval excrement, making it ideal for the development of 1-DNJ-related goods.

A Neural Model for Insect Steering Applied to Olfaction and Path Integration.

Many animal behaviors require orientation and steering with respect to the environment. For insects, a key brain area involved in spatial orientation and navigation is the central complex. Activity in this neural circuit has been shown to track the insect's current heading relative to its environment and has also been proposed to be the substrate of path integration. However, it remains unclear how the output of the central complex is integrated into motor commands. Central complex output neurons project to the lateral accessory lobes (LAL), from which descending neurons project to thoracic motor centers. Here, we present a computational model of a simple neural network that has been described anatomically and physiologically in the LALs of male silkworm moths, in the context of odor-mediated steering. We present and analyze two versions of this network, one rate based and one based on spiking neurons. The modeled network consists of an inhibitory local interneuron and a bistable descending neuron (flip-flop) that both receive input in the LAL. The flip-flop neuron projects onto neck motor neurons to induce steering. We show that this simple computational model not only replicates the basic parameters of male silkworm moth behavior in a simulated odor plume but can also take input from a computational model of path integration in the central complex and use it to steer back to a point of origin. Furthermore, we find that increasing the level of detail within the model improves the realism of the model's behavior, leading to the emergence of looping behavior as an orientation strategy. Our results suggest that descending neurons originating in the LALs, such as flip-flop neurons, are sufficient to mediate multiple steering behaviors. This study is therefore a first step to close the gap between orientation circuits in the central complex and downstream motor centers.

The differences in cocoon and silk qualities among sex-related mulberry and silkworm feeding groups.

Sexual dimorphism is seen in many dioecious plant and animal species, which may influence their trophic interactions. The differences in trophic interactions derived from sexual dimorphism in plants may influence herbivorous performance and population dynamics. Both silkworm (Bombyx mori L.) and mulberry (Morus alba L.) usually exhibit sexual dimorphism. However, few studies have been conducted on the effect of sex-related silkworm and mulberry pairings on cocoon and silk qualities, which are crucial in sericulture. Here, we compared the differences in cocoon and silk qualities under four feeding combinations (FS-FL: female silkworm fed with leaves from female mulberry trees; MS-FL: male silkworm fed with leaves from female mulberry trees; FS-ML: female silkworm fed with leaves from male mulberry trees; MS-ML: male silkworm fed with leaves from male mulberry trees). The results showed that silkworms exhibited male-biased herbivory with more male mulberry leaves digested. The FS-ML group had higher silk weight and silk ratio of fresh cocoons than the FS-FL group, and the MS-ML group had lower coarse points than the MS-FL group. Compared with groups FS-ML and FS-FL, both MS-FL and MS-ML had smaller cocoons with longer silk lengths and a higher silk ratio of the fresh cocoons. In addition, the Entropy Weight-TOPSIS method showed the cocoon quality rank as FS-ML > FS-FL > MS-FL > MS-ML, whereas silk quality rank was MS-ML > FS-FL > FS-ML > MS-FL. These results indicate that the quality of cocoon and silk is related to the interaction of silkworm and mulberry at the sex level. Furthermore, female silkworms fed with female and male tree leaves have a higher total yield in cocoon production, while male silkworms fed with male tree leaves produced higher silk quality.

Midgut and Head Transcriptomic Analysis of Silkworms Reveals the Physiological Effects of Artificial Diets.

Simple SummaryAs oligophagous insects, silkworms have a very limited diet. Here, the transcriptomic characteristics of the head and midgut tissues of fifth instar male and female silkworms were studied to elucidate the effects of different food structures on silkworm innate immunity. Compared with mulberry leaves, artificial diets decreased the expression levels of immune-associated genes, thus decreasing silkworm immunity and susceptibility to pathogen infections.Silkworms, a model lepidopteran insect, have a very simple diet. Artificial diets as an alternative nutrient source for silkworms are gradually being developed. To understand the effects of various nutrients on the growth and development of silkworms, we studied the transcriptomic differences in the midgut and head tissues of male and female silkworms fed either fresh mulberry leaves or artificial diets. In the artificial diet group, compared with the control group (fed mulberry leaves), 923 and 619 differentially expressed genes (DEGs) were identified from the midgut, and 2969 and 3427 DEGs were identified from the head, in female and male silkworms. According to our analysis, the DEGs were mainly involved in the digestion and absorption of nutrients and silkworm innate immunity. These experimental results provide insights into the effects of different foods, such as artificial diets or fresh mulberry leaves, on silkworms.

Effects of Serine/Arginine Enriched Protein BmUP on the Development of Male Silkworm Reproductive Organs

Serine/arginine-rich proteins are a class of highly conserved splicing factor proteins involved in constitutive and alternative splicing. We screened a low molecular weight serine/arginine rich protein from silkworms and named it BmUP. Temporal and spatial expression analysis indicated that the BmUP gene was specifically expressed in the silkworm testis, and the highest expression occurred in the pre-pupa stage from the fifth instar to the moth stages. Here, we generated BmUP knockout individuals with the CRISPR/Cas9 system. Both the internal and external genitalia of knockout individuals were abnormal in knockout compared with wild-type male silkworms. In transgenic silkworms overexpressing BmUP, male silkworms showed a phenotype similar to that of the knockout individuals, whereas female individuals showed no significant differences from the wild type. In addition, by conducting promoter analysis, we identified Bmachi, a transcription factor that regulates the BmUP gene. Gel migration experiments revealed that BmAchi specifically binds the BmUP promoter. Quantitative real-time PCR showed that an increase in Bmachi expression up-regulated the expression of BmUP. In contrast, when the expression of Bmachi decreased, the expression of BmUP also downregulated in the experimental group compared with the control group. These results provide new insights for studying the effects of serine/arginine-rich proteins on the development of silkworm genitals.

Long Non-Coding RNA Bmdsx-AS1 Effects on Male External Genital Development in Silkworm.

Simple SummaryLncRNAs are a class of non-coding RNAs longer than 200 nt that are involved in a variety of biological processes. Studies on lncRNAs in Bombyx mori have shown that some lncRNAs are involved in brain development, silk production and the response to virus infection of the host. However, the roles of lncRNAs are still largely unknown in the silkworm. In this study, we analyzed the function of lncRNAs Bmdsx-AS1 in silkworm by transgenic overexpression, which not only affects the development of male silkworm external genitalia, but also participates in the regulation of EGFR signaling pathway. Moreover, we studied the upstream promoter of Bmdsx-AS1 and found that the BmAbd-B transcription factor of the Hox gene family can negatively regulate the expression of Bmdsx-AS1. These results laid a substantial foundation for in-depth study of the function of lncRNAs in the silkworm.Long non-coding RNAs (lncRNAs) have been suggested to play important roles in some biological processes. However, the detailed mechanisms are not fully understood. We previously identified an antisense lncRNA, Bmdsx-AS1, that is involved in pre-mRNA splicing of the sex-determining gene Bmdsx in the silkworm. In this study, we analyzed the changes in the male external genitalia of transgenic overexpressed Bmdsx-AS1 silkworm lines and analyzed downstream and upstream responses. We found that Bmdsx-AS1 transgenic silkworms, compared with wild type, showed more claspers in the male external genitalia. Quantitative real-time PCR (qPCR) results indicated that overexpression of Bmdsx-AS1 decreased the expression of genes in the EGFR signaling pathway. Knockdown of Bmdsx-AS1 increased the activity of the EGFR pathway. Through promoter prediction, promoter truncation and electrophoretic mobility shift assay (EMSA) analyses, we found that the protein encoded by the Hox gene BmAbd-B specifically binds to the promoter of Bmdsx-AS1. Moreover, overexpression of BmAbd-B in the silkworm BmE cell line indicated that BmAbd-B negatively regulates the mRNA expression of Bmdsx-AS1. Our study provides insights into the regulatory mechanism of the lncRNA in the silkworm.

On-line identification of silkworm pupae gender by short-wavelength near infrared spectroscopy and pattern recognition technology

The gender identification of silkworm pupae is a critical step in the sericulture industry's breeding process. In this study, a low cost, short-wavelength (815-1075 nm) near infrared (NIR) spectrometer combined with multivariate spectra evaluation methods was used to establish calibration models for the on-line identification of female and male pupae of eight silkworm varieties. The diffuse reflection short-wavelength spectra were recorded, and then principal component analysis (PCA), linear discriminant analysis (LDA), and partial least squares discriminant analysis (PLSDA) were tested for calibration model development. The PCA and LDA results showed, that spectral differences between the female and male silkworm pupae existed, however, the two evaluation techniques could not separate the female and male silkworm pupae with the required accuracy. The PLSDA calibration models, on the other hand, could separate the pupae according to their gender with the necessary prediction accuracy of >98%. Thus, it has been proved, that a low-cost, short-wavelength range NIR spectrometer in combination with a PLSDA calibration routine can be successfully applied for the reliable on-line identification of female and male silkworm pupae.

Resveratrol elongates the lifespan and improves antioxidant activity in the silkworm Bombyx mori

A number of research has shown that the plant polyphenol resveratrol, one of the most prominent small molecules, has beneficial protective effects in multiple organisms, including worms, flies, and killifish. To understand the effects of resveratrol on lifespan, we evaluated its effects in the silkworm Bombyx mori. In this study, we found that lifespan was significantly prolonged in both female and male silkworms treated with resveratrol. Silkworm larval weight was significantly increased from day 3 of the 5th larval instar (L5D3) to day 7 of the 5th larval instar (L5D7). However, the weight of the pupa, cocoon, and total cocoon was not significantly different in female silkworms with resveratrol treatment than that in controls. Meanwhile, resveratrol significantly improved the thermotolerance of the silkworms, which enhanced their survival rate. Moreover, antioxidant activity was increased by resveratrol in both female and male silkworms. Furthermore, an antioxidant-related signalling pathway, SIRT7-FoxO-GST, was activated in silkworms with resveratrol treatment. Collectively, these results help us to understand the molecular pathways underlying resveratrol induced pro-longevity effects and indicate that silkworm is a promising animal model for evaluating the effects of lifespan-extending drugs.

Characterization of 10 Biogenic Amines in Male Silkworm Moth by HPLC with Precolumn Derivatization

Background: Biogenic Amines (BAs) are biologically active nitrogenous organic compounds of low molecular weight, which are frequently found in a wide variety of foods, beverages and herbs due to their toxic potential in humans. Male Silkworm Moth (MSM), a Traditional Chinese Medicine (TCM), has been exploited and utilized as nutritious liquor based on its traditional effects in the Chinese community. Objective: The objective of this study was to develop an HPLC with Dns-Cl derivatization method for characterizing overall BAs in MSM and providing data for further evaluating its activities and safety profiles. Methods: The method has acceptable sensitivity, precision, accuracy, selectivity and recovery, and was successfully applied to the determination of the BAs contents in MSM for the first time. Results: In the analysis of 10 batches of MSM samples, serotonin and dopamine were not found in detectable concentrations in any samples, and the most abundant amine found was putrescine. The mean values of tryptamine, phenylethylamine, putrescine, cadaverine, histamine, tyramine, spermidine, and spermine determined in the samples were found to be 34.7 mg/ kg, 16.1 mg/ kg, 218.3 mg/ kg, 37.9 mg/ kg, 12.1 mg/ kg, 18.2 mg/ kg, 4.5mg/ kg, and 0.9 mg/ kg, respectively. Conclusion: The contents of BAs in 10 batches of MSM were below the maximum recommended limits, and MSM can be used safely.

Fibroblast growth factor 21 prolongs lifespan and improves stress tolerance in the silkworm, Bombyx mori.

BackgroundFibroblast growth factor 21 (FGF21), an FGF family member, is an atypical hormone and pro-longevity factor.MethodsTo better understand of the effects of exogenous administration of FGF21 on lifespan and stress tolerance, and the underlying molecular basis, we used the silkworm, Bombyx mori, as an experimental animal model to evaluate FGF21’s pharmaceutical effects.ResultsLifespan was significantly prolonged in female silkworms with FGF21 replenishment, whereas no effect was observed in the male silkworms. FGF21 replenishment also significantly improved the activity of antioxidant systems such as glutathione-S-transferase (GST) and superoxide dismutase (SOD) and significantly decreased malondialdehyde (MDA) content. Moreover, FGF21 was found to play a critical role in enhancing stress resistance, including ultraviolet (UV) irradiation tolerance and thermotolerance. Furthermore, AMPK, FoxO, and sirtuins were activated by FGF21 and may be responsible for the prolonged lifespan and enhanced antioxidant activity observed in silkworms.ConclusionsCollectively, the results suggest the molecular pathways underlying of FGF21-induced longevity and stress tolerance, and support the use of silkworms as a promising experimental animal model for evaluating the pharmaceutical effects of small molecules.

Analysis of the effects of nanosilver on bacterial community in the intestinal fluid of silkworms using high-throughput sequencing.

Nanosilver is an environment-friendly, harmless alternative of traditional disinfectants which can be potentially applied in the sericulture industry. However, the effects of nanosilver on the intestinal bacterial community of the silkworms (Bombyx mori L.) are unclear. In this study, Illumina MiSeq high-throughput sequencing technology was used to assess the intestinal bacterial community in both male and female silkworms while treated with different concentrations of nanosilver. We found that nanosilver significantly influenced the composition of silkworm intestinal bacterial community on the different taxonomic levels. Most conspicuously, the abundance of Firmicutes was increased by the treatment of 20 mg L-1 nanosilver but decreased by that of 100 mg L-1 nanosilver at the phylum level. The same trend was observed in Bacilli at the class level and in Enterococcus at the genus level. In some extreme cases, application of nanosilver eliminated the bacterium, e.g., Brevibacillus, but increased the population of several other bacteria in the host intestine, such as Blautia, Terrisporobacter, Faecalibacterium, and some bacteria could only be found in nanosilver treatment groups, e.g., Dialister. In addition, although nanosilver generally showed negative effects on the cocooning rate in a dose-dependent manner, we found that 20 mg L-1 nanosilver treatment significantly increased the body weight of silkworms and did not show negative effects on the survival rate. These results indicated that the intestinal bacteria community of silkworm larvae was significantly changed after nanosilver treatment which might consequently influence host growth and development.

An antisense lncRNA functions in alternative splicing of Bmdsx in the silkworm, Bombyx mori

Long non-coding RNAs (lncRNAs) are recently thought to play important roles in some physiological processes. In this study, we identified a lncRNA (named as Bmdsx-AS1) which is the antisense transcript and locates on the position of the crucial sex-determining gene Bmdsx in the silkworm. Quantitative real-time PCR and Fluorescence in situ hybridization demonstrated that Bmdsx-AS1 was highly expressed in the silkworm testis. After knock-down of Bmdsx-AS1, the splicing pattern of Bmdsx pre-mRNA was altered in male silkworm. Transgenic overexpression of Bmdsx-AS1 indicated male-specific splicing form of Bmdsx arose in female silkworm. Furthermore, by using RNA-protein pull down, LC-MS/MS and EMSA, we found a splicing factor hnRNPH binding specifically to Bmdsx-AS1. Co-Immunoprecipitation suggested that hnRNPH interacted with BmPSI, one of the upstream regulating factors of Bmdsx. Thus, our results suggested that the antisense lncRNA Bmdsx-AS1 was involved in alternative splicing of Bmdsx in the silkworm.

Analysis of the role of wind information for efficient chemical plume tracing based on optogenetic silkworm moth behavior

Many animals use olfactory information to search for feeding areas and other individuals in real time and with high efficiency. We focus on the chemical plume tracing (CPT) ability of male silkworm moths and investigate an efficient CPT strategy for an autonomous robot. In the case of flying insects, the wind direction is an important factor in CPT, because the wind carries odors amongst other environmental information. However, whether the same phenomenon occurs in the walking silkworm moth has not been investigated. Therefore, we examine how the silkworm moth uses wind information during CPT. To accurately investigate the response to the wind direction, we introduce an optogenetic approach that replaces the odor stimulation with light stimulation, allowing us to separate the ‘wind stimulus’ from the ‘odor stimulus’. We examine how the moth uses wind direction information in a biological experiment, and find that the movement speed is significantly reduced when the wind speed is relatively fast (1.0 m s−1). By implementing this phenomenon in an autonomous robot, we can improve the successful search rate over that of the conventional moth-inspired algorithm. Regarding the search time, the proposed algorithm finds the odor source faster in a low-frequency odorant emission environment, whereas the search is slower than the conventional method when the odor frequency is higher. Therefore, switching from the use of wind direction information to odor information according to the frequency with which the odor is encountered leads to efficient CPT performance.

Modeling of the Adaptive Chemical Plume Tracing Algorithm of an Insect Using Fuzzy Inference

In this paper, we focus on the chemical plume tracing (CPT) problem, which is a known engineering challenge. In nature, animals solve the CPT by adaptively modifying their behavior according to the environment. Therefore, we propose a CPT solution method with high engineering value by modeling the CPT algorithm of an animal. In this paper, we consider a male silkworm moth as a model. To perform CPT in a turbulent environment, the adaptive selection of the behavior plays an important role. Therefore, we performed simultaneous measurement experiments involving CPT behavior of the brain and analyzed the links between the brain's neural activity and behavioral patterns. We measured the brain's neural response in the lateral accessory lobe (LAL), which generates motion commands. We employed fuzzy inference to analyze the relationship between CPT behavior and LAL neural activity. As a result of analyzing the relationship between CPT behavior and LAL, we found that the moth modulates the behavior of the state transition probability depending on the odor frequency. We modeled the obtained phenomenon and verified its effectiveness through a constructive method. As a result, the search performance was improved compared to the conventional moth algorithm.