During major flood events, waterborne contaminants are relatively poorly characterized. This is due to logistical difficulties associated with obtaining water samples in potentially dangerous flood conditions. Herein, we report analyses of water samples from a large, flooded landscape in Victoria, Australia, during a major flood event. We collected 83 samples from seven rivers and 18 river locations as far apart as 520 km. The sampling campaign covered a 26-day window, with 3 samples taken weekly from each site. Floodwater samples were analyzed for 778 contaminants and 544 microbial species were identified using eDNA. Our study shows that 85 contaminants were detected in floodwaters. Fungicides, phthalates, plant macronutrients, metal(loid)s and PPCPs were better explained by land uses, whereas herbicides and insecticides were explained by a mixture of land use and water flow data. Potentially pathogenic orders with the highest detection rates were Enterobacterales (82.4%), Mycobacteriales (70.6%) and Legionellales (58.8%). Contaminants and microbial signatures responded to rainfall, water flow and water level, demonstrating increased and varied human and environmental risks of exposure during the sampling window. Our work underlines the importance of rigorous and timely monitoring and provides an evidence-base for decision making during increasingly frequent and intense climate driven flood events.
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