The ability of alkaloids of the aerial part of Genista tinctoria L. (a dye dye) collected during the flowering period in the Alsheevsky district of the Republic of Bashkortostan, to inhibit the reproduction of the influenza A (H1N1) virus pdm09 was studied. The component composition of the sum of alkaloids and individual fractions is determined by the chromatography-mass spectrometric method. Cytotoxicity and anti-influenza properties of G. tinctoria alkaloids have been studied in vitro in the culture of MDCK cells with respect to the strain of influenza A / California / 07/09 (H1N1) pdm09, rimantadine was used as the reference preparation. Based on the data obtained, a 50% cytotoxic dose of C50, a 50% inhibitory dose of IC50, and a SI selectivity index characterizing the selectivity of the action of the sample against the virus were calculated. According to the data obtained, fraction 4 of the sum of alkaloids of G. tinctoria (SI = 10), containing alkaloids 12-N-methylcytisine, (-)-cytisin, d-lupanin, 12-N-formylcytisine, anagirine, baptilin, and fraction 5 (SI = 7) containing (-)-cytisin, anagirine and baptiphlin. Anagyrin is a major component of fraction 4, its content is 0.13% of the weight of air-dry raw materials, and baptilin-5, its content is 0.14%. It should be noted that, in comparison with the sum of G. tinctoria alkaloids and fractions 2 and 3, fractions 4 and 5 enriched with tetracyclic quinolizidine alkaloids (anagirine and baptifolin) show more pronounced anti-influenza activity. The revealed regularity can serve as the basis for further optimization of the anti-influenza properties of these alkaloids by means of their chemical transformations.