The environmental quality of the learning environment is a critical element for good health and well-being among beneficiaries of tertiary institutions, ultimately influencing staff productivity and learning performance of students. High population densities, inefficient resource use and unique microenvironments within tertiary institutions can result in myriad environmental challenges which require targeted solutions. An environmental gap analysis is a pragmatic and evidence-based approach that can be used to evaluate the environmental quality of institutions through the provision of baseline data and comparison to relevant environmental standards. In this study, an environmental gap analysis was conducted at university campus, where air, noise, water and soil data were collected for 12weeks at indoor and outdoor sites. The data obtained were averaged, graphed and benchmarked using relevant local and international guidelines to identify gaps. Additionally, the data were tested using two-way analysis of variance (ANOVA). Generally, the levels of parameters monitored were acceptable and within respective local and international guidelines; however, air temperature and relative humidity of the indoor cafeteria as well as turbidity of tap water were not in conformance with guidelines. Improvements in maintenance of ventilation systems and water distribution infrastructure are recommended along with further investigations of drinking water and indoor air quality at the campus. The insight obtained in this study provided important baseline data, in an area where there is a dearth of information, from which deficiencies can be identified and evidence-based recommendations can be made.