Mainstream Deammonification Research Articles

Enhanced nitrogen removal and microbial community of the mainstream deammonification treating fluctuating influent C/N wastewater by the novel functional carriers

The plug-flow fixed bed reactors with zeolite/tourmaline-modified polyurethane carriers (PFBRZTP) and polyurethane carriers (PFBRPU) were operated to assess the fluctuating influent C/N impact on the system performance and the carrier effect on the enhancing the system operation. Result suggested that fluctuations in influent C/N and variations in operational temperature reduced the removal performance and system stability within PFBRPU. The negative impact of C/N fluctuation could be effectively mitigated by effluent reflux. In contrast, PFBRZTP performance and operational stability of maintained at high level with a greater nitrogen removal rate (0.18 kg N·(m³·d)−1). Redundancy analyses showed that the fluctuations in influent C/N dramatically affected the microbiome structure in PFBRPU, and the leading influencing factor was shifted to the fluctuating amount of influent C/N, which in turn reduced the system performance and stability. ZTP carriers could maintain the balance of main functional bacterial activity and abundance and promote the partial denitrification process with a higher Thauera abundance of 0.48%.

The enhanced biomass attachment of novel functional carriers and its effect on facilitating the start-up of mainstream deammonification at ambient temperature

Two novel functional carriers, including zeolite-modified polyurethane (ZP) carriers and zeolite/tourmaline-modified polyurethane (ZTP) carriers, were proposed to enhance biomass attachment and the rapid establishment of mainstream deammonification. Results showed that the surface roughness, specific surface area and Zeta potential of the ZTP carrier increased by 13.6 times, 2.24 times and 17.54 mV compared to the polyurethane (PU) carriers, and its surface metal element content also increased. The biomass increase rate and content on a ZTP carrier reached 0.431 mg VSS·d−1 and 42.3 ± 3.1 mg VSS, respectively, which were 1.74 and 2.50 times higher, compared with that on a PU carrier. Furthermore, the plug-flow fixed-bed reactor with ZTP carriers (PFBRZTP) was constructed for the mainstream deammonification in 21 d, with a final NRR of 0.18 kg N·(m3·d)−1. The AnAOB abundance (Candidatus Brocadia and Candidatus Kuenenia-dominated) reached an abundance of 9.4 %, and ammonium-oxidizing bacteria (AOB) (Nitrosomonas) reached an abundance of 4.0 %. Overall, the ZTP carriers have great engineering applications and promising perspective for enriching AnAOB and rapid establishing the mainstream deammonification.

Sustainable mainstream deammonification by ion exchange and bioregeneration via partial nitritation/anammox

The partial nitritation and anammox (PN/A) process has gained popularity for the treatment of nitrogen removal in wastewater due to significant energy savings and its potentially much lower CO2 footprint. However, the treatment of mainstream municipal wastewater by PN/A has been limited mainly due to its unsuitable composition. In this research, we apply ion exchange using a zeolite column to selectively remove and concentrate ammonium from mainstream municipal wastewater. After an absorption phase, the ion exchange column is regenerated using a brine solution. The ammonium rich brine is “bioregenerated” in a PN/A reactor where ammonium is converted to nitrogen gas allowing the brine to be reused in another cycle of ion exchange regeneration. To successfully remove ammonium from the spent brine, anammox and ammonia oxidizing bacteria (AOB) were first cultivated in separate reactors under hypersaline conditions (4.0 %) and later combined in a single PN/A reactor. After continuous operation with sea water, the PN/A reactor treated recirculating brine from the ion exchange column for 48 cycles of ammonium absorption and bioregeneration with minimal blowdown. The various cations of the regenerant solution were stable except for calcium that reached very high values upwards of 3000 mg/L as Ca2+ and finally caused PN/A reactor failure due to mineral precipitation. The buildup of high concentrations of calcium in the regenerant was addressed in two ways: 1) 20 % regenerant replacement per cycle, and 2) precipitation of CaCO3 via the addition of sodium carbonate. Both methods were applied to 30 absorption and bioregeneration cycles each and shown to be effective in keeping calcium concentrations from accumulating in the regenerant allowing for stable PN/A reactor operation.

Mainstream deammonification at ambient temperature treating real sewage by a plug-flow fixed-bed reactor based on zeolite/tourmaline-modified polyurethane carriers

A plug-flow fixed-bed reactor (PFBR) with zeolite/tourmaline-modified polyurethane (ZTP) carriers (PFBRZTP) was constructed to realize mainstream deammonification for real domestic sewage treatment. The PFBRZTP and PFBR were operated for 111 days treating aerobically pretreated sewage in parallel. A higher nitrogen removal rate of 0.12 kg N·(m3·d)−1 was achieved in PFBRZTP despite lowering the temperature (16.8–19.7 °C) and fluctuating water quality. Meanwhile, it was indicated that anaerobic ammonium oxidation dominated (64.0 ± 13.2%) in PFBRZTP, by nitrogen removal pathway analysis and high anaerobic ammonium-oxidizing bacteria (AnAOB) activity (2.89 mg N·(g VSS·h)−1). And, the lower protein/polysaccharides (PS) ratio further indicated a better biofilm structure in PFBRZTP owing to a higher abundance of microorganisms relevant to PS and cryoprotective EPS secretion. Furthermore, partial denitrification was an important nitrite supply process in PFBRZTP based on low AOB activity/AnAOB activity ratio, higher Thauera abundance and a remarkably positive correlation between Thauera abundance and AnAOB activity.

Multi-parameter control-based operation strategy for mainstream deammonification in an integrated anaerobic biofilm reactor-step feed MBR

The mainstream deammonification of municipal wastewater has been recognized as one of the greatest challenges in wastewater engineering. The conventional activated sludge process has disadvantages of high energy input and sludge production. To tackle this situation, an innovative A-B process, where an anaerobic biofilm reactor (AnBR) functioned as the A stage for energy recovery, and a step-feed membrane bioreactor (MBR) functioned as the B stage for mainstream deammonification, was constructed for carbon-neutral wastewater treatment. For addressing the challenge associated with selective retention of ammonia-oxidizing bacteria (AOB) over nitrite oxidizing bacteria (NOB), a multi-parameter control-based operation strategy was developed with synergistic control of influent COD redistribution, dissolved oxygen (DO) concentration and sludge retention time (SRT) in the innovative AnBR – step-feed MBR system. Results showed that more than 85% of wastewater COD could be removed with the direct production of methane gas in the AnBR. A relatively stable partial nitritation, which is a prerequisite of anammox, was achieved with the successful suppression of NOB, leading to 98% of ammonium-N and 73% of total nitrogen removed. Anammox bacteria could well survive and enrich in the integrated system, and the contribution of anammox to the total nitrogen removal was more than 70% at optimal conditions. Reactions network involved in the nitrogen transformation in the integrated system was further constructed through the mass balance and microbial community structure analyses. Consequently, this study demonstrated a practically feasible process configuration with high operation and control flexibility towards stable mainstream deammonification of municipal wastewater.

Concept development of a mainstream deammonification and comparison with conventional process in terms of energy, performance and economical construction perspectives.

Deammonification for nitrogen removal in municipal wastewater in temperate and cold climate zones is currently limited to the side stream of municipal wastewater treatment plants (MWWTP). This study developed a conceptual model of a mainstream deammonification plant, designed for 30,000 P.E., considering possible solutions corresponding to the challenging mainstream conditions in Germany. In addition, the energy-saving potential, nitrogen elimination performance and construction-related costs of mainstream deammonification were compared to a conventional plant model, having a single-stage activated sludge process with upstream denitrification. The results revealed that an additional treatment step by combining chemical precipitation and ultra-fine screening is advantageous prior the mainstream deammonification. Hereby chemical oxygen demand (COD) can be reduced by 80% so that the COD:N ratio can be reduced from 12 to 2.5. Laboratory experiments testing mainstream conditions of temperature (8-20°C), pH (6-9) and COD:N ratio (1-6) showed an achievable volumetric nitrogen removal rate (VNRR) of at least 50 gN/(m3∙d) for various deammonifying sludges from side stream deammonification systems in the state of North Rhine-Westphalia, Germany, where m3 denotes reactor volume. Assuming a retained Norganic content of 0.0035 kgNorg./(P.E.∙d) from the daily loads of N at carbon removal stage and a VNRR of 50 gN/(m3∙d) under mainstream conditions, a resident-specific reactor volume of 0.115 m3/(P.E.) is required for mainstream deammonification. This is in the same order of magnitude as the conventional activated sludge process, i.e., 0.173 m3/(P.E.) for an MWWTP of size class of 4. The conventional plant model yielded a total specific electricity demand of 35 kWh/(P.E.∙a) for the operation of the whole MWWTP and an energy recovery potential of 15.8 kWh/(P.E.∙a) through anaerobic digestion. In contrast, the developed mainstream deammonification model plant would require only a 21.5 kWh/(P.E.∙a) energy demand and result in 24 kWh/(P.E.∙a) energy recovery potential, enabling the mainstream deammonification model plant to be self-sufficient. The retrofitting costs for the implementation of mainstream deammonification in existing conventional MWWTPs are nearly negligible as the existing units like activated sludge reactors, aerators and monitoring technology are reusable. However, the mainstream deammonification must meet the performance requirement of VNRR of about 50 gN/(m3∙d) in this case.

Enhanced nitrogen removal in mainstream deammonification systems at ambient temperature by novel modified carriers and differentiation of microbial community transformation

Zeolite-modified polyurethane (ZP) carriers and zeolite/tourmaline-modified polyurethane (ZTP) carriers were proposed to enhance mainstream deammonification. The system with ZTP carriers was rapidly established in 28 days with a nitrogen removal rate (NRR) of 0.150 kg N·(m3·d)−1. Moreover, the facilitative effect of tourmaline was suggested by the highest humic acid peak intensity and more balanced potential activity. Besides, SEM-EDS analysis revealed carrier characteristic improvement was achieved in both novel carriers while maintaining an excellent spatial structure. Moreover, the microbial analysis suggested that both modified carriers support the substrate supply to anaerobic ammonium oxidizing bacteria (AnAOB) by enhancing dissimilatory nitrate reduction to ammonium and partial denitrification under nitrate accumulation conditions. Nevertheless, the ZTP system had a greater advantage over maintaining the original AnAOB (Candidatus Jettenia) and ammonium oxidizing bacteria (Nitrosomonas) abundance. Overall, this study provides ZTP carriers with great potential for facilitating the establishment of mainstream deammonification at full-scale WWTPs.

Microbial response on the first full-scale DEMON® biomass transfer for mainstream deammonification

Sidestream partial nitritation and deammonification (pN/A) of high-strength ammonia wastewater is a well-established technology. Its expansion to the mainstream is, however mainly impeded by poor retention of anaerobic ammonia oxidizing bacteria (AnAOB), insufficient repression of nitrite oxidizing bacteria (NOB) and difficult control of soluble chemical oxygen demand and nitrite levels.At the municipal wastewater treatment plant in Strass (Austria) the microbial consortium was exhaustively monitored at full-scale over one and a half year with regular transfer of sidestream DEMON® biomass and further retention and enrichment of granular anammox biomass via hydrocyclone operation.Routine process parameters were surveyed and the response and evolution of the microbiota was followed by molecular tools, ex-situ activity tests and further, AnAOB quantification through particle tracking and heme measurement.After eight months of operation, the first anaerobic, simultaneous depletion of ammonia and nitrite was observed ex-situ, together with a direction to higher nitrite generation (68% of total NOx-N) as compared to nitrate under aerobic conditions. Our dissolved oxygen (DO) scheme allowed for transient anoxic conditions and had a strong influence on nitrite levels and the NOB community, where Nitrobacter eventually dominated Nitrospira. The establishment of a minor but stable AnAOB biomass was accompanied by the rise of Chloroflexi and distinct emergence of Chlorobi, a trend not seen in the sidestream system. Interestingly, the most pronounced switch in the microbial community and noticeable NOB repression occurred during unfavorable conditions, i.e. the cold winter season and high organic load. Further abatement of NOB was achieved through bioaugmentation of aerobic ammonia oxidizing bacteria (AerAOB) from the sidestream-DEMON® tank. Performance of the sidestream pN/A was not impaired by this operational scheme and the average volumetric nitrogen removal rate of the mainstream even doubled in the second half of the monitoring campaign. We conclude that a combination of both, regular sidestream-DEMON® biomass transfer and granular SRT increase via hydrocyclone operation was crucial for AnAOB establishment within the mainstream.

Performance evaluation and model-based optimization of the mainstream deammonification in an integrated fixed-film activated sludge reactor

This study aimed to model and optimize mainstream deammonification in an integrated fixed-film activated sludge (IFAS) pilot plant under natural seasonal temperature variations. The effect of gradually decreasing temperature on the performance was evaluated during a winter season and a transition period to summer conditions, and the correlation of the performance parameters was investigated using principal component analysis (PCA). The optimization of intermittent aeration in the long-term (30 days) dynamic conditions with on/off ratio and dissolved oxygen (DO) set-point control was used to maximize the N-removal rate (NRR) and N-removal efficiency (NRE). Optimization results (DO set-point of 0.2–0.25 mgO2/L, and on/off ratio of 0.05) increased the NRE and NRR of total inorganic N (daily average) from 30% to > 50% and 15 gN/m3d to 25 gN/m3d, respectively. This novel long-term optimization strategy is a powerful tool for enhancing the efficiency in mainstream deammonification.

Multi-Parameter Control-Based Operation Strategy for Mainstream Deammonification of Municipal Wastewater in an Integrated Anaerobic Biofilm Reactor-Step Feed Mbr Process

Multi-Parameter Control-Based Operation Strategy for Mainstream Deammonification of Municipal Wastewater in an Integrated Anaerobic Biofilm Reactor-Step Feed Mbr Process

Achieving rapid mainstream deammonification through inoculating long-term refrigerated sidestream sludge in plug-flow fixed-bed biofilm reactor

The start-up of a stable mainstream deammonification requires sufficient anaerobic ammonia-oxidizing bacteria (AnAOB). This study used a plug-flow fixed-bed reactor (PFBR) to verify the feasibility of establishing the mainstream deammonification system by inoculating the sidestream sludge after long-term refrigeration. A rapid resuscitation of the mainstream deammonification system was accomplished by controlling the front-end aeration rate of the PFBR. Results showed that the system was rapidly resuscitated in 44 days eventually with the nitrogen removal rate and nitrogen removal efficiency of 0.1 kg N·(m3·d)−1 and 79.1%, respectively. Also, the efficient performance was secured by the proportionate approaching equilibrium of AnAOB and ammonia-oxidizing bacteria (AOB) activity of 2.35 ± 0.40 and 2.60 ± 0.29 mg N·(g VSS·h)−1, respectively. In addition, Pearson correlation analysis revealed that AnAOB abundance (detected Candidatus Kuenenia) negatively correlated with the AOB (mainly Nitrosomonas)/AnAOB abundance ratio, while correlated positively with the residual ammonium concentration of a region. Furthermore, long-term refrigeration probably reduced the cross-feed relationship between AnAOB and other symbiotic organisms (Armatimonadetes and Chloroflexi) to maintain the basic metabolism. Meanwhile, extracellular polymeric substances produced by other genera (order Xanthomonadales and Pseudomonadales) decreased the mass transfer, protecting AnAOB from unfavorable conditions, thereby facilitating high AnAOB abundance during refrigeration. Thus, this study provides a promising perspective towards the practical applications of mainstream process.

Role of air scouring in anaerobic/anoxic tanks providing nitrogen removal by mainstream anammox conversion in a hybrid biofilm/suspended growth full-scale WWTP in China.

A full-scale wastewater treatment plant in China experienced unintentional anammox bacterial enrichment on biofilm carriers placed in the anaerobic and anoxic zones of an anaerobic/anoxic/oxic process under ambient temperatures and without bioaugmentation. Here, we show that microaerophilic conditions resulting from air scouring needed for biofilm carrier suspension in the anaerobic/anoxic zones can support a robust nitritation/anammox process. Results from an in situ on/off air scouring test showed that air scouring strongly induced both ammonia and total inorganic nitrogen removal in the anaerobic/anoxic zones. Ammonium concentration in the anaerobic and anoxic tanks remained constant or even slightly increased when air scouring was off, indicating that air scouring made a noticeable difference in nitrogen profiles in the anaerobic/anoxic zones. Various batch tests further indicated that partial denitrification is not likely to generate nitrite for anammox bacteria. Robust nitritation, and anammox on the carriers, can occur at low dissolved oxygen conditions, as measured in the full-scale facility. The observations show that mainstream deammonification without sidestream bioaugmentation at moderate temperature is feasible and further optimization by a more dedicated design can result in improved nitrogen removal in cases when chemical oxygen demand is limited in mainstream wastewater treatment. PRACTITIONER POINTS: Microaerophilic conditions in a full-scale IFAS reactor caused mainstream anammox in moderate temperate area. Robust nitritation, and anammox on the carriers, can occur at low dissolved oxygen conditions in anaerobic/anoxic tanks with air scouring. Anammox can function well with conventional nitrification and denitrification process at mainstream conditions for stable nitrogen removal.

Insights into two stable mainstream deammonification process and different microbial community dynamics at ambient temperature

How to achieve stable mainstream deammonification is still a huge challenge. In this work, satisfactory nitrogen removal were achieved in a deammonification granular sludge reactor (R1, 0.42 ± 0.03 kg N / (m3·d)) and a mixed flocculent with granular sludge reactor (R2, 0.39 ± 0.04 kg N / (m3·d)) at ambient temperature (21–28 ℃) . The good adaptability of anammox bacteria (Candidatus Jettenia) to ambient temperature ensured its efficient activity (0.84–1.54 mg N/(g VSS·h)). The overexpression ammonia monooxygenase gene abundances in ammonia oxidizing bacteria (Nitrosomonas) was also predicted. The inhibition of hydrazine and the competition of denitrifying bacteria (Denitratisoma) to nitrite nitrogen, leading to a low Nitrospira relative abundances (0.2%-2.1%) . It was also found that R1 was more resistant to the unfavorable condition. For R2, higher Denitratisoma abundances (9.2%-18.5%) and predicted metabolic pathway abundances related to carbon metabolism were observed.

Primary sludge fermentate as carbon source for mainstream partial denitrification-anammox (PdNA).

Primary sludge fermentate, a concentrated hydrolyzed wastewater carbon, was evaluated for use as an alternative carbon source for mainstream partial denitrification-anammox (PdNA) in a suspended growth activated sludge process in terms of partial denitrification (PdN) efficiency, PdNA nitrogen removal contributions, and final effluent quality. Fermenter operation at a 2-day sludge retention time (SRT) resulted in the maximum achievable yield of 0.14±0.05gsCOD/gVSS without release of excessive ammonia and phosphorus to the system. Based on the results of batch experiments, fermentate addition led to PdN efficiency of 93±14%, which was similar to acetate at a nitrate residual of 2-3mgN/L. In the pilot-scale mainstream deammonification reactor, PdN efficiency using fermentate was 49±24%, which was lower than acetate (66±24% during acetate period I and 70±21% during acetate period II), most probably due to lower nitrate and ammonium kinetics in the PdN zone. Methanol cost-saving potential for the application of PdNA as the main short-cut nitrogen pathway was estimated to be 30% to 55% depending on the PdN efficiency achieved. PRACTITIONER POINTS: Primary sludge fermentate was evaluated as an alternative carbon source for mainstream partial denitrification-anammox (PdNA). Fermenter operated at a 1 to 2 day SRT resulted in the maximum achievable yield without the release of excessive ammonia and phosphorus to the system. Although 93% partial denitrification efficiency was achieved with fermentate in batch experiments, around 49% PdN efficiency was achieved in pilot studies. Application of PdNA with fermentate can result in significant methanol cost savings.

Modelling AOB-NOB competition in shortcut nitrification compared with conventional nitrification-denitrification process

In particular, mainstream deammonification and/or shortened nitrification-denitrification via nitrite (so-called “nitrite shunt”) is a promising new treatment concept that has the potential to revolutionise how nitrogen removal is achieved at WWTPs. Understanding the role of the AOB/NOB competition in the nitrogen cycle in wastewater treatment systems will change operational strategies of the novel nitrogen removal processes. The key role in this process is inhibition of NOB activity undesirably affects AOB activity and leads to inefficient partial nitrification process and when used as pre-treatment for Anammox it can limit nitrite supply to Anammox bacteria. Successful NOB repression requires a combination of such factors as a low DO concentration, a rapid transition from aerobic to anoxic conditions, and tight control of Temperature and/or pH. The major driving force behind the successful NOB washout is the inhibition of those bacteria based on the difference in the growth rate between AOB and NOB. The obtained results from this study show the mechanisms and operating conditions (e.g. DO concentration, Temp.) leading to complete domination of AOB over NOB under aerobic conditions. This paper presents the perspectives on modelling AOB-NOB competition in shortcut nitrification. The combined deammonification, shortened nitrification-denitrification and/or nitritation-anammox process was compared with conventional nitrification-denitrification based on own experiments and literature data. Its successful application as shortcut nitrification technology and new control system will represent a paradigm shift for the wastewater industry, offering the opportunity for sustainable wastewater treatment, energy-neutral or even energy-positive facilities, and substantial reductions in treatment costs.

Mutual Interaction between Temperature and DO Set Point on AOB and NOB Activity during Shortcut Nitrification in a Sequencing Batch Reactor in Terms of Energy Consumption Optimization

Recently, many wastewater treatment plants (WWTPs) have had to deal with serious problems related to the restrictive requirements regarding the effluent quality, as well as significant energy consumption associated with it. In this situation, mainstream deammonification and/or shortened nitrification-denitrification via nitrite (so-called “nitrite shunt”) is a new promising strategy. This study shows the mechanisms and operating conditions (e.g., dissolved oxygen (DO) concentration, temp.), leading to the complete domination of ammonium oxidizing bacteria (AOB) over nitrite oxidizing bacteria (NOB) under aerobic conditions. Its successful application as shortcut nitrification in the sequencing batch reactor (SBR) technology will represent a paradigm shift for the wastewater industry, offering the opportunity for efficient wastewater treatment, energy-neutral or even energy-positive facilities, and substantial reductions in treatment costs. In this study, under low and moderate temperatures (10–16 °C), averaged DO concentrations (0.7 mg O2/L) were preferable to ensure beneficial AOB activity over NOB, by maintaining reasonable energy consumption. Elevated temperatures (~30 °C), as well as increased DO concentration, were recognized as beneficial for the NOB activity stimulation, thus under such conditions, the DO limitation seems to be a more prospective approach.

Achieving stable mainstream deammonification process by a novel combinatorial control strategy

In this study, a novel combinatorial control strategy was developed to guarantee a stable mainstream deammonification process, with three critical steps including (a) upflow airwater washing, (b) short-term increased nitrogen loading rate (NLR), and (c) low oxygen supply. Results showed that two upflow double-blanket filter (UDBF) reactors effectively performed the mainstream deammonification process with the nitrogen removal efficiency (NRE) 84.5 ± 2.2% and 84.6 ± 1.6%, respectively and nitrogen removal rate (NRR) 123.8 ± 2.9 and 125.5 ± 6.2 g N·(m3·d)−1, respectively. Statistically, temperature and C/N were considered as two vital factors affecting the nitrogen removal pathways, which co-explained 80.9% and 78.4% of the maximum possible contribution of heterotrophic denitrification in both reactors. The deammonification process accounted for more than 59.8% of TN removal in R2 and 54.8% in R1, which cooperated well with heterotrophic denitrification for efficient performance in treating municipal sewage.

The effect of the COD: N ratio on mainstream deammonification in an integrated fixed-film activated sludge sequencing batch reactor

For eight months, a sequencing batch reactor (SBR) with integrated fixed-film activated sludge (IFAS) was operated in ambient temperature to study engineering and practical aspects of application of deammonification for mainstream conditions. For biofilm formation, K3 Kaldnes carriers were used, where the anaerobic ammonium oxidation (anammox) process can occur in deep layers of biofilm, while partial nitritation occurs in oxygen-rich outer layers. After the initial running phase of the reactor (Phase 1) to provide time for microorganisms to adapt, the COD: N ratio increased to around 2.6 in Phase 2 through reducing the ammonium concentration and increasing COD in synthetic wastewater to get closer to mainstream conditions. The total reaction time in each half-day batch cycle was kept 625 min throughout various phases, but the duration of intermittent aeration was regulated at 4 ± 1 min. While final nitrogen removal efficiency (NRE) for Phase 1 was 43%, at the end of Phase 2, it decreased to 37%. However, a maximum NRE at 90% was achieved during Phase 2. The identification of the responsible microorganisms was made through Fluorescence in situ hybridization (FISH), while Mixed Liquor Suspended Solid (MLSS) and Mixed Liquor Volatile Suspended Solid (MLVSS) was used to estimate the physical presence of bacteria. Ammonium oxidizing bacteria (AOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) were dominant bacteria, respectively. The adverse effects of a gradual increase of COD: N ratio from 0.17 to more than 2.0 caused a decline in NRE to around 15%.

Feasibility of Mainstream Deammonification for Advanced Municipal Wastewater Treatment

Feasibility of Mainstream Deammonification for Advanced Municipal Wastewater Treatment

Performance and microbial community analysis of two sludge type reactors in achieving mainstream deammonification with hydrazine addition

The deammonification process is a promising and energy efficient nitrogen removal technology. Since deammonification process has succeeded in high-strength ammonia nitrogen wastewater treatment (sidestream deammonification) but its application in treating low-strength ammonium nitrogen wastewater (mainstream deammonification) remains a great challenge. In this study, mainstream deammonification process in two reactors maintained stability with hydrazine (N2H4) addition. The two reactors consisted of a deammonification granular reactor and a mixed ammonia oxidizing bacteria (AOB) flocculent with anaerobic ammonia oxidizing bacteria (AnAOB) granular reactor. Deammonification granular reactor had a more efficient total nitrogen removal efficiency (TNRE, 80.5 ± 5.8%) and nitrogen removal rate (NRR, 0.33 ± 0.04 g/(L·day)). The advantage of retain biomass in granular sludge reactor lead to a more balanced ex-situ activity between AOB (0.37 mg N/(g VSS·h)) and AnAOB (0.43 mg N/(g VSS·h)). Candidatus Brocadia and Nitraspira were detected the dominant genus responsible for the observed AnAOB and nitrite oxidizing bacteria (NOB), respectively. The more obvious effect of N2H4 on enhancing AnAOB and suppressing NOB both in ex-situ activity and genus abundances in mixed sludge reactor were also founded may due to loose spatial distribution among species.