Main Root Research Articles

RrWRKY1, a Transcription Factor, Is Involved in the Regulation of the Salt Stress Response in Rosa rugosa.

Salt stress has become a major environmental problem affecting plant growth and development. Some WRKY transcription factors have been reported to be involved in the salt stress response in plants. However, there are few studies on the involvement of WRKYs in the salt stress response in Rosa rugosa. In this study, we isolated a salt tolerance gene, RrWRKY1, from R. rugosa. RrWRKY1 was found to belong to Group I of the WRKY family, and it was specifically expressed in leaves and petals. RrWRKY1 expression was upregulated under NaCl stress in rose leaves. After silencing RrWRKY1 in R. rugosa, transgenic plants showed dry leaves and black and brown veins, indicating sensitivity to salt stress. At the same time, the transcription levels of the salt tolerance-related genes RrNHX1, RrABF2, RrRD22, RrNCED1, and RrHKT1 also changed significantly. The superoxide dismutase (SOD) and peroxidase (POD) activities decreased, the proline content decreased, and the malondialdehyde (MDA) content in the gene-silenced plants increased, indicating that RrWRKY1 regulates the salt tolerance of R. rugosa. In addition, the overexpression of RrWRKY1 in Arabidopsis thaliana improved the germination rate and the average of the main root and lateral root lengths, and the transgenic plants had a larger number of lateral roots than the WT plants under salt stress. This study provides candidate gene resources for salinity tolerance breeding and a theoretical basis for analyzing the salinity tolerance mechanism of the WRKY gene.

Distribution characteristics of photoassimilates in walnut leaves to different organs

The understanding of photoassimilate distribution serves as the fundamental basis for scientific regulation of fruit quality. Currently, there is a scarcity of research on whole-plant scale photoassimilate distribution in walnut. In order to clarify the characteristics of leaf photoassimilates translocation to various organs in 5-year-old 'Wen185' (J. regia 'Wen185') walnut during the growing season, this study used the 13C isotope pulse labeling technique to label the whole plant of walnut trees in the growing season, temporal variations of 13C abundance (δ13C), 13C partition rate (R13C), leaf source strength and fruit sink strength were analyzed in various organs at different days after tree flowering. The findings indicated that during the periods of 30–70 days and 90–110 days after flowering, there was a higher distribution of 13C in fruits and vegetative branches. However, at 110–130 days after flowering, the predominant allocation of 13C shifted towards main trunk and roots. In-depth study of source leaves and sink fruits showed that chlorophyll content in leaves increased significantly 30–50 days after anthesis, indicating that they gradually became mature functional leaves. The increase of net photosynthetic rate led to increase of source strength, and the retention of photoassimilates in leaves was higher at this time. From 30 to 70 days after flowering, the fresh weight and volume of fruit increased rapidly, which increased the capacity of the sink and enhanced the competition ability against photoassimilates. The recovery of photosynthetic capacity of leaves from 90 to 110 days promoted the output of photoassimilates. At this time, walnut entered the oil conversion period, and the demand for photoassimilates increased. All these factors jointly promoted the unloading of photoassimilates in fruit. In summary, maintaining adequate material conditions and optimizing tree structure at 30-70d and 90-110d after anthesis are important for more efficient distribution of photoassimilates to fruit.

Biological Acclimatization of Micropropagated Al-Taif Rose (Rosa damascena f. trigintipetala (Diek) R. Keller) Plants Using Arbuscular Mycorrhizal Fungi Rhizophagus fasciculatus

Tissue culture is used to multiply Al-Taif rose (Rosa damascena f. trigintipetala (Diek) R. Keller) plants in order to meet the demands of the fragrance, cosmetic, and floriculture industries. The use of arbuscular mycorrhizal fungus (AMF) could potentially improve plant growth and acclimatization performance to ex vitro conditions. Thus, in the current study, we investigated how AMF Rhizophagus fasciculatus influences the growth, establishment, and physiological performance of micropropagated Al-Taif rose plants during the acclimatization stage. The growth and physiological parameters of the AMF-treated plants were evaluated after a 12 week growth period in the growth chambers. The plants treated with AMF exhibited greater height (25.53 cm) and biomass growth values for both shoot fresh weight (0.93 g/plant) and dry weight (0.030 g/plant), more leaves (11.3/plant), more leaf area (66.15 cm2), longer main roots (15.05 cm/plant), total root length (172.16 cm/plant), total root area (64.36 cm2/plant), and biomass from both fresh weight (383 mg/plant) and dry weight (80.00 mg/plant) of the plants. The plants treated with AMF also exhibited increased rates of net CO2 assimilation, stomatal conductance, and transpiration compared to the control plants. The proline content in the leaves and roots was significantly lower in the AMF-treated plants than untreated plants. The Fv/Fm ratio, which serves as an indicator of the intrinsic or maximal efficacy of Photosystem II (PSII) demonstrated a notable decline in the untreated Al-Taif rose plants. These results elucidate the advantageous impact of AMF colonization on micropropagated Al-Taif rose plants, thereby enhancing their resilience against adverse ex vitro conditions.

Potential Identification of Root System Architecture Using GPR for Tree Translocation as a Sustainable Forestry Task: A Case Study of the Wild Service Tree

Sustainable economic development serves society but requires taking over space, often at the expense of areas occupied by single trees or even parts of forest areas. Techniques for transplanting adult trees used in various conflict situations at the interface of economy and nature work as a tool for sustainable management of urbanized and industrial areas, as well as, in certain circumstances, forest or naturally valuable areas. This study aimed to evaluate the effectiveness of ground-penetrating radar (GPR) in determining the horizontal and vertical extent of tree root systems before transplantation. Employing this non-invasive method to map root system architecture aids in the appropriate equipment selection and helps define the dimensions and depth of trenches to minimize root damage during excavation. This study specifically focused on the root systems of wild service trees (Sorbus torminalis (L.) Crantz) found in a limestone mine area, where some specimens were planned to be transplanted, as the species is protected under law in Poland. The root systems were scanned with a ground-penetrating radar equipped with a 750 MHz antenna. Then, the root balls were dug out, and the root parameters and other dendrometric parameters were measured. The GPR survey and manual root analyses provided rich comparative graphic material. The number of the main roots detected by the GPR was comparable to those inventoried after extracting the stump. The research was carried out in problematic soil, causing non-standard deformations of the root systems. Especially in such conditions, identifying unusually arranged roots using the GPR method is valuable because it helps in a detailed planning of the transplanting process, minimizing root breakage during the activities carried out, which increases the survival chances of the transplanted tree in a new location.

Probiotic Enterococcus Faecium Attenuated Atherosclerosis by Improving SCFAs Associated with Gut Microbiota in ApoE-/- Mice.

Atherosclerosis, as the main root cause, makes cardiovascular diseases (CVDs) a substantial worldwide health concern. Inflammation and disrupted cholesterol metabolism are the primary clinical risk elements contributing to the onset of atherosclerosis. Few works exist on the improvement effect of gut microbiota on atherosclerosis. One specific probiotic strain, Enterococcus faecium NCIMB11508, has shown promise in mitigating inflammation. Consequently, it is critical to investigate its potential in reducing the progression of atherosclerosis. In our study, we administered E. faecium NCIMB11508 orally to ApoE-/- mice, resulting in a decrease in the formation of atherosclerotic lesions. Additionally, it demonstrated the ability to lower the inflammatory factor levels both in the aorta and blood serum while maintaining the integrity of the small intestine against lipopolysaccharides. Moreover, E. faecium NCIMB11508 had a beneficial impact on the gut microbiota composition by increasing the levels of short-chain fatty acids (SCFAs), which in turn helped to reduce inflammation and protect the intestine. The probiotic E. faecium NCIMB11508, according to our research, has a definitive capacity to prevent atherosclerosis progression by beneficially altering the SCFA composition in the gut microbiota of ApoE-/- mice.

When Co-Generative Learning Met Co-Production and Co-Creation - On How Three Different Concepts Shaped “Samskaping”

AbstractThe Norwegian/Danish word «samskaping” is today one of the most frequently used terms or concepts in the public discourse of development and change. Scholars familiar with the work of Morten Levin and Max Elden will recognize “samskaping” as a short translation of their model of “cogenerative learning”, originating from the discussion in the 80’s and 90’ on participative action research processes. However, there is no simple line of transfer of ideas from “cogenerative learning” in action research to “samskaping” in public sector today. Rather, three concepts from different disciplines all ended up being translated into or strongly associated with the newly constructed and popular Norwegian word “samskaping”, and Morten Levin’s “cogenerative learning” is one of the three. The two others are co-production and co-creation, which originates from fields of innovation research in private and public sector. Seen from the position of action research and the heritage from Morten Levin, this means that although his (and Max Eldens) concept (in the Norwegian translation) appears to have gained a strong impact Norway the last decade, the meaning and importance of the concept is partially lost to many of its later users. This article will track the main roots of “samskaping” as it has found its way in the everyday language of change and development in Norway, and will in particular try to reclaim for the term or concept, some of the main themes that shaped the discussion and the design of the co-generative learning model in PAR, in particular participation, power and social change.

Sexual dimorphism at different life stages: early life sexual differences in root growth in Silene latifolia.

Male and female dioecious plants often show sexual dimorphism, differing in morphological, physiological and life-history traits. Most previous studies have focused on differences between males and females during or after reproduction, paying little attention to the pre-reproductive stages of the individuals. Here we assessed the response of male and female individuals of the dioecious plant Silene latifolia to abiotic stress at different life stages, including pre-reproductive (i.e. seedlings and young plants) and reproductive individuals. We measured growth, resource allocation and discrimination against 13C under nutrient deficiency, water stress, as well as their interaction. We observed sexual dimorphism in root growth, with female seedlings having longer main roots than male plants. Pre-reproductive male and female plants also responded differently, in terms of root allocation, to nutrient and water availability. At reproduction, females grew more roots than males when water was not limiting. These differences could help explain the female-skewed sex ratios found in natural populations of S. latifolia. We found no evidence of sexual dimorphism in aboveground dry mass, although females had longer leaves than males at the seedling stage. We conclude that sexual dimorphism in S. latifolia may occur not as a consequence of reproduction, but well before it.

The efect of low-dose gamma radiation on the development of plants from irradiated Pisum sativum sprouts in laboratory experiments

The problem of radiation exposure to organisms is relevant for areas where nuclear industry enterprises are located. Plant biotests are widely used for biotesting of anthropogenic factors affecting the environment, including radiation. The sensitivity of growth and cytogenetic parameters of the biotest based on Pisum sativum to the action of gamma irradiation n has been shown before, but only at high doses. The purpose of this work was to evaluate the influence of low-dose gamma irradiation on the development of Pisum sativum over 6-10 days after irradiating the sprouts. In the experiments, seeds of the Radomir sowing variety from the collection of the pea breeding laboratory of Krasnoyarsk Research Institute of Agriculture - Federal Research Centre Krasnoyarsk Scientific Center SB RAS were used. Pea sprouts were irradiated with a 137Cs source for 24 and 72 hours, with the absorbed dose being 20 and 62 mGy. After irradiation, the sprouts were grown in a climatic chamber on hydroponics for 10 days (240 hours). The control consisted of non-irradiated sprouts grown under identical conditions. The experiments for the first time provided reliable data on the negative effects of gamma irradiation (20 and 62 mGy) on the development of sowing peas 6-10 days after irradiation. A negative effect of gamma irradiation on the growth of the main and lateral roots of young plants was revealed. The experiments with irradiated pea sprouts confirmed the previously known fact that plant roots are more sensitive to irradiation compared to shoots. The pattern of change in the length of the main root of peas over time during germination at a dose of 20 mGy is described by a linear equation, while for a higher dose of irradiation, 62 mGy, the data can be approximated by a logarithmic equation with saturation. The different character of root length dependence on the time of cultivation after irradiation indicates a possible trend in plant growth under various doses of irradiation and cultivation times.

A novel wheel wear indicator in regard to wheel-rail contact parameters and vehicle hunting stability

It is well known that the abnormal equivalent conicity caused by wheel and rail wear is the main root of hunting instability, but it is still difficult to trace the underlying factor when the dynamic instability occurs, that is, wheel wear induced, rail wear induced or both. To face this challenge problem, this paper constructed a novel wheel wear indicator based on the moved wear area difference. The long-term tracking test data of different wheel profiles were used to analyze the correlation between the wear indicators and the wheel-rail contact parameters. A fully detailed dynamic model of one typical high-speed railway vehicle was developed to investigate the evolution of vehicle hunting stability under the variation of the proposed wear indicator. The results indicate that the proposed wear indicator based on the moved wear area difference has a strong correlation with the equivalent conicity and can be used for the estimation of wheel-rail contact conditions. Furthermore, the hunting stability and the instability form is closely related to the value of the proposed wheel wear indicator, which is helpful to trace the underlying factor of hunting instability, thereby supporting intelligent operation and maintenance of railway vehicles.

Attitude of Onion Growers towards Drip Irrigation System in Tamil Nadu, India

The drip irrigation system is a kind of micro-irrigation system that has the potential to salvage the water and other nutrients by entrance water to drip gradually to the main roots if plants and other plant parts, which is from on the soil surface or buried inside the soil surface. The main aim of drip irrigation is to place water directly into the main root zone and decrease the water vapour. This study investigates the attitude of Onion growing farmers towards drip irrigation system. The survey was conducted to collect primary data from 119 onion growing farmers in Thondamuthur block of Coimbatore district. Findings reveal that less than three-fifths (57.14%) of the respondents expressed neutral attitude level towards drip irrigation system followed by 21.85 per cent had favourable and 21.01 per cent of the respondents had un-favourable attitude level towards drip irrigation system respectively. This study recommends the implementation of an extensive agricultural institution with more prevalent and periodic visits to drip owners should be established in order to provide regular technical advice and valuable data on the maintenance and repair of the drip irrigation scheme. Such initiatives could enhance the livelihood status of the onion growing farmers in Tamil Nadu.

Effectiveness of supplementary antimicrobial procedures in disinfecting lateral canals as evaluated by a novel ex vivo analytical approach

This ex vivo study devised an analytical ex vivo method for infection/disinfection of simulated lateral canals located in the middle and apical segments of the root. The antibacterial effects of supplementary approaches were tested in this model. Extracted mandibular premolars had their main root canals enlarged and then two lateral canals (100 μm in diameter) were created in the root, one in the apical and the other in the middle portion. Micro-computed tomography was used for specimen selection and to confirm the quality of the simulated ramifications. The specimens were contaminated with a mixed bacterial culture from subgingival bacterial biofilm added to pure Enterococcus faecalis strain ATCC 29212 grown overnight, using special strategies to facilitate culture medium penetration within the lateral canals. The following procedures were tested for disinfection: NaOCl/passive ultrasonic irrigation (PUI), NaOCl/XP-endo Finisher, ozonated water/continuous ultrasonic irrigation (CUI), and NaOCl/conventional irrigation with 30-G needles (control). Bacteriological samples were taken from the main canal before (S1) and after (S2) each supplementary protocol, and also from each lateral canal after treatment (S3). DNA extracted from the samples was subjected to quantitative real-time polymerase chain reaction. All S1 main canal samples were positive for bacterial presence. Bacterial counts in the main root canal substantially decreased by 99.2% after PUI, 99.1% after ozone/CUI, 99% after XP-endo Finisher, and 96% in the control group (P < 0.01 for all groups). There were no significant differences between groups (P > 0.05). The same was observed when comparing the effects of the supplementary approaches in the apical and middle lateral canals (P > 0.05). Only a few lateral canals showed no detectable bacteria. The method proposed here proved effective for ex vivo infection/disinfection studies. All supplementary approaches induced a substantial bacterial reduction in the main canal, with no significant differences between them. However, in terms of lateral canal disinfection, none of the tested approaches showed significant effects when compared to the control group.

Blood flow assessment of gastric tube with indocyanine green fluorescence angiography and postoperative endoscopy during esophagectomy: indocyanine green enhancement time indicated congestion

BackgroundDuring esophagectomy, evaluation of blood supply to the gastric tube is critically important to estimate and avoid anastomotic complications. This retrospective study investigated the relationship between indocyanine green (ICG) fluorescence angiography during esophagectomy and postoperative endoscopy findings, especially mucosal color change.MethodsThis study retrospectively collected data from 86 patients who underwent subtotal esophagectomy and reconstruction using a gastric tube for esophageal cancer at the Tokyo Medical and Dental University between 2017 and 2020. The flow speed of ICG fluorescence in the gastric tube was evaluated during the operation. Additionally, the main root of ICG enhancement and pattern of ICG distribution in the gastric tube were evaluated. On postoperative day 1 (POD1), the change in the mucosal color to white, thought to reflect ischemia, or black, thought to reflect congestion of the proximal gastric tube, was evaluated. The correlations between these factors, clinical parameters, and surgical outcomes were evaluated. Univariate and multivariate analyses used logistic regression to identify the risk factors affecting mucosal color change.ResultsMultivariate analyses revealed that the only independent significant predictor of mucosal congestion on POD1 was the ICG enhancement time of the right gastric tube tip (odds ratio, 14.49; 95% confidential interval, 2.41–87.24; P = 0.004).ConclusionsThis study indicated that the ICG enhancement time is related to venous malperfusion and congestion rather than arterial malperfusion and ischemia.

Design of a 2R Open-Chain Plug Seedling-Picking Mechanism and Control System Constrained by a Differential Non-Circular Planetary Gear Train

With a focus on the problems of complex structure and accumulated lateral clearance in the single degree of freedom non-circular wheel system seedling-picking mechanism, which leads to poor motion accuracy, trajectory, and attitude, this study developed a 2R open-chain chili plug seedling-picking mechanism (SPM) constrained by a differential non-circular wheel system. The picking arm was driven by a single-stage non-uniform speed transmission mechanism to reproduce the seedling-picking trajectory and attitude. A protruding seedling-picking device, SPM control system, and test bench were designed. A kinematic model of a differential non-circular gear system was established, and an optimization design software for the SPM was developed based on kinematic analysis. The kinematic characteristics of the SPM were analyzed under optimal parameters. This study completed the seedling-picking performance test of the SPM on the control panel. The test showed that the designed chili SPM can sequentially complete the processes of seedling picking, conveying, retracting, pushing, and returning under the automatic control of the test bench without damaging the main root. The lateral root damage rate was 15.7%, effectively ensuring the integrity of the seedling bowl substrate.

Effects of container size and growing media on growth of argan (Argania spinosa) seedlings in Morocco

Background: Argan (Argania spinosa) is an important arid species in Morocco, hence the need to undertake nursery practices that promote its regeneration and sustainability in a vulnerable environment. This study aimed to examine the effect of container size and growing medium on morphological traits, biomass, as well as root morphology of one-year-old argan seedlings in the Marrakech region of Morocco. Methods: Three container sizes of 300, 400, and 500 cm3 were selected in which seven types of growing media with varying proportions of acacia compost, crushed cypress cones, Barbary thuja, eucalyptus capsule composts, and potting soil were used. Subsequently, measurements of plant growth variables and biomass, as well as identification of root deformations were carried out. Results: Our results show that container size and growth medium strongly influenced (p &lt; 0.001) morphological traits and biomass of argan seedlings. Growth was highest in seedlings grown in the 500 cm3 container on substrates composed of both 100% acacia compost and 50% potting soil + 50% acacia compost, while it was lowest in seedlings in the 300 cm3 container with the compost composed of a mixture of equal proportions of cypress and Barbary thuja, and eucalyptus. While the main root deformities were hooked roots (14.1%) and root eccentricity (13.0%), seedlings with multiple deformities were by far the most common (38.4%). The size of the container did not seem to influence their occurrence, whereas about 35% of the deformations were observed in seedlings grown on substrates composed of mixtures of potting soil and acacia compost, cypress cones, and Barbary thuja and eucalyptus composts. Conclusions: This study shows the importance of adopting an adequate protocol to allow the regeneration of forest species with the necessary characteristics to survive the often-challenging environmental conditions prevailing not only in southern Morocco but also across most of the country.

Mechanisms of ROS-mediated interactions between Bacillus aryabhattai LAD and maize roots to promote plant growth

BackgroundPlant growth-promoting rhizobacteria (PGPR), as a group of environmentally friendly bacteria growing in the rhizosphere of plants, play an important role in plant growth and development and resistance to environmental stresses. However, their limited understanding has led to the fact that their large-scale use in agriculture is still scarce, and the mechanisms by which beneficial bacteria are selected by plants and how they interact with them are still unclear.MethodIn this study, we investigated the interaction between the auxin-producing strain Bacillus aryabhattai LAD and maize roots, and performed transcriptomic and metabolomic analyses of Bacillus aryabhattai LAD after treatment with maize root secretions(RS).ResultsOur results show that there is a feedback effect between the plant immune system and bacterial auxin. Bacteria activate the immune response of plant roots to produce reactive oxygen species(ROS), which in turn stimulates bacteria to synthesize IAA, and the synthesized IAA further promotes plant growth. Under the condition of co-culture with LAD, the main root length, seedling length, root surface area and root volume of maize increased by 197%, 107%, 89% and 75%, respectively. In addition, the results of transcriptome metabolome analysis showed that LAD was significantly enriched in amino acid metabolism, carbohydrate metabolism and lipid metabolism pathways after RS treatment, including 93 differentially expressed genes and 45 differentially accumulated metabolites.ConclusionOur findings not only provide a relevant model for exploring the effects of plant-soil microbial interactions on plant defense functions and thereby promoting plant growth, but also lay a solid foundation for the future large-scale use of PGPR in agriculture for sustainable agricultural development.

Effect of pH on growth and Cd accumulation in different rice varieties under hydroponics

ABSTRACT Currently, applying lime to cadmium (Cd)-contaminated paddy fields to increase pH and reduce Cd availability is an effective method to control excessive Cd levels in rice grain. However, under hydroponic conditions, the impact of increased pH on Cd accumulation in different rice varieties remains unclear. This study employed three rice varieties (Yuzhenxiang, Shaoxiang 100, Xiangwanxian 12) with different Cd accumulation characteristics under different pH and long-term treatment with 1 μM CdCl2, to study the effect of pH on growth and Cd accumulation in different rice varieties. The result showed that as pH shifted from 5 to 8, the SPAD values, shoot dry weight, and plant height of the three rice varieties significantly decreased. The main root length, root volume, and root dry weight of Yuzhenxiang, and Shaoxiang100 significantly decreased. Conversely, the root architecture indicators of Xiangwanxian 12 did not change significantly. As for element accumulation, increasing the pH significantly increased the content of Mn in both the shoots and roots of all three varieties. Yuzhenxiang significantly reduced Cd content in both the shoots and roots of rice, while Shaoxiang100 significantly increased Cd content in both parts. Xiangwanxian 12 showed a significant increase in Cd content in the shoots but a decrease in the roots. In terms of subcellular distribution, Yuzhenxiang significantly reduced Cd concentrations in the cell wall and organelles of root cells, resulting in lower Cd concentrations in the root tissue. Conversely, Shaoxiang100 significantly increased Cd concentrations in the cell wall, organelles, and soluble fractions of root cells, leading to higher Cd concentrations in the root tissue. Xiangwanxian 12 also exhibited a decrease in Cd concentrations in the cell wall, organelles, and soluble fraction of root cells, resulting in lower Cd concentrations in the root tissue. Additionally, the expression of the OsNRAMP5 and OsHMA3 gene was significantly increased in Shaoxiang 100, while no significantly change in Yuzhenxiang and Xiangwanxian 12. These results provide important guidance on the impact of pH on Cd accumulation during the vegetative growth stage of different rice varieties.

Agrotechnical practices for improving the quality of seed stocks in the nursery

The paper presents the preliminary results of an assessment of some agrotechnical techniques for improving the quality of seed stocks for pears based on quince of ordinary VNIISPK breeding. Studies have shown that the use of bedding material introduced into rows during sowing in the form of sphagnum moss or sand contributed to better branching of the primary root of the seedling, and, consequently, the formation of more skeletal roots than in control variants. The morphological structure of the sand favored high aeration and waterproofing with excess moisture in the root zone of the germinating seed. The skeletal root branched better than in the control. However, the main amount of branching was concentrated on the endings of the skeletal root or close to it. The places of branching of skeletal roots from the main root of the seedling often remained open, which subsequently may lead to a decrease in the anchoring of graft-rootstock combinations in the garden. On the other hand, having a high moisture-retaining ability, aeration and antiseptic properties, sphagnum moss had an even more beneficial effect on the young sprout and subsequently contributes to the formation of a branched root system of the seedling with many overgrown roots. The number of skeletal roots and the order of their branching exceeded the control variants by 1.5–2.0 times. Almost the entire skeletal root branched, which is very valuable for survival, plant fixation in the soil, nutrition and moisture supply during the growing season. Based on the conducted research, in order to improve the quality of seed stocks based on quince, when sowing, bedding material in the form of sand or sphagnum moss should be added to the rows, contributing to better branching of the roots of quince seedlings.

Major root endodontic treatments: a systematic review

Introduction: In the root canal treatment scenario, irrigation is one of the most important aspects of the biomechanical preparation of the root canal. A condition for successful endodontic retreatment is adequate cleaning of the root canals, therefore, special attention should be paid to the technique used to remove the filling material, the most commonly used being cement, pastes, and gutta-percha cones. Objective: This study aimed to present the main root endodontic treatments to analyze and compare the techniques for removing pulp tissue debris resulting from root preparation and microorganisms from the canals of the root canal system, seeking complete cleaning and asepsis. Methods: The PRISMA Platform systematic review rules were followed. The search was conducted from April to July 2024 in the Scopus, PubMed, Science Direct, Scielo, and Google Scholar databases. The quality of the studies was based on the GRADE instrument and the risk of bias was analyzed according to the Cochrane instrument. Results and Conclusion: 129 articles were found, 39 were evaluated and 21 were included in this systematic review. Considering the Cochrane tool for risk of bias, the global assessment resulted in 25 studies with a high risk of bias and 27 studies that did not meet GRADE. Most studies showed homogeneity in their results, with X2 =91.5% &gt;50%. It was concluded that the system of root canal instrumentation with rotary files maintains the quality of root preparation and, at the same time, reduces the number of files required to obtain a canal, which would consequently reduce the operative time and also considerably reduce the risk of torsion fracture within the root canal than with files. Irrigation plays a fundamental role in the success of endodontic treatment. Although hypochlorite is the most important irrigant solution, no irrigant can perform all the tasks required by irrigation. A detailed understanding of the mode of action of various solutions is important for optimal irrigation. Within the limitations of this study, the use of the self-adjusting file with the combination of EDTA and NaOCl improved Ca(OH)2 removal. Passive ultrasonic irrigation and the self-adjusting file were more effective in removing Ca(OH)2 from the lateral sulci in the apical parts of the root canal than the EndoVac and conventional syringe irrigation systems.

Light at the end of the tunnel: integrating signaling pathways in the coordination of lateral root development.

Root system architecture (RSA) encompasses a range of physical root attributes, including the lateral roots (LRs), root hairs and adventitious roots, in addition to the primary or main root. This overall structure is a crucial trait for efficient water and mineral capture alongside providing anchorage to the plant in the soil and is vital for plant productivity and fitness. RSA dynamics are dependent upon various environmental cues such as light, soil pH, water, mineral nutrition and the belowground microbiome. Among these factors, light signaling through HY5 significantly influences the flexibility of RSA by controlling different signaling pathways that converge at photoreceptors-mediated signaling, also present in the 'hidden half'. Furthermore, several phytohormones also drive the formation and emergence of LRs and are critical to harmonize intra and extracellular stimuli in this regard. This review endeavors to elucidate the impact of these interactions on RSA, with particular emphasis on LR development and to enhance our understanding of the fundamental mechanisms governing the light-regulation of LR growth and physiology.

Combination of Near-Infrared Spectroscopy and Multisource Data Fusion for the Geographical Origin Authentication of Panax notoginseng

Panax notoginseng (Burk.) F.H. Chen (P. notoginseng), a precious medicinal herb, provides both considerable values. There is confusion in the market regarding the geographical origins of P. notoginseng. It is of paramount importance to authenticate geographical origins of P. notoginseng, which will facilitate the rational utilization of resources and sustainable development. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were employed to investigate the influence of the tissue and data fusion strategy for the authentication of P. notoginseng origins. The PLS-DA model employing the main root spectra demonstrated great potential for authenticating P. notoginseng origin. The PLS-DA training and test sets based on feature-level fusion (VIP) spectral data were 100% accurate, indicating excellent potential in authenticating P. notoginseng. The data fusion strategy improved the authentication. The article presents a reliable and efficacious novel methodology for authenticating P. notoginseng origins and a scientific foundation for its quality control.