Neurons In Main Olfactory Bulb Research Articles

Steady-state centrifugal input via the lateral olfactory tract modulates spontaneous activity in the rat main olfactory bulb

Mitral and tufted cells in the main olfactory bulb (MOB) of anesthetized rats exhibit vigorous spontaneous activity, action potentials produced in the absence of odor stimuli. The central hypothesis of this paper is that tonic activity of centrifugal input to the MOB modulates the spontaneous activity of MOB neurons. The spontaneous activity of centrifugal fibers causes a baseline of steady-state neurotransmitter release, and odor stimulation produces transient changes in the resulting spontaneous activity. This study evaluated the effect of blocking centrifugal axon conduction in the lateral olfactory tract (LOT) by topically applying 2% lidocaine. Mean spontaneous activity of single bulbar neurons was recorded in each MOB layer before and after lidocaine application. While the spontaneous activity of most MOB neurons reversibly decreased after blockade of the LOT, the spontaneous activity of some neurons in the mitral, tufted and granule cell layers increased. The possible mechanisms producing such changes in spontaneous activity are discussed in terms of the tonic, steady-state release of excitatory and/or inhibitory signals from centrifugal inputs to the MOB. The data show for the first time that tonic centrifugal input to the MOB modulates the spontaneous activity of MOB interneurons and projection neurons. The present study is one of the few that focuses on steady-state spontaneous activity. The modulation of spontaneous activity demonstrated in this study implies a behaviorally relevant, state-dependent regulation of the MOB by the CNS.

The basal forebrain modulates spontaneous activity of principal cells in the main olfactory bulb of anesthetized mice

Spontaneous activity is an important characteristic of the principal cells in the main olfactory bulb (MOB) for encoding odor information, which is modulated by the basal forebrain. Cholinergic activation has been reported to inhibit all major neuron types in the MOB. In this study, the effect of diagonal band (NDB) stimulation on mitral/tufted (M/T) cell spontaneous activity was examined in anesthetized mice. NDB stimulation increased spontaneous activity in 66 MOB neurons which lasted for 2–35 s before returning to the baseline level. The majority of the effected units showed a decrease of interspike intervals (ISI) at a range of 8–25 ms. Fifty-two percent of NDB stimulation responsive units showed intrinsic rhythmical bursting, which was enhanced temporarily by NDB stimulation, whereas the remaining non-rhythmic units were capable of synchronized bursting. The effect was attenuated by scopolamine in 21 of 27 units tested. Only four NDB units were inhibited by NDB stimulation, an inhibition that lasted less than 10 s. The NDB stimulation responsive neurons appeared to be M/T cells. Our findings demonstrate an NDB excitation effect on M/T neurons that mostly requires muscarinic receptor activation, and is likely due to non-selectivity of electrical stimulation. This suggests that cholinergic and a diverse group of non-cholinergic neurons in the basal forebrain co-ordinately modulate the dynamics of M/T cell spontaneous activity, which is fundamental for odor representation and attentional perception.

Optogenetic Activation of Basal Forebrain Cholinergic Neurons Modulates Neuronal Excitability and Sensory Responses in the Main Olfactory Bulb

The main olfactory bulb (MOB) in mammals receives massive centrifugal input from cholinergic neurons in the horizontal limb of the diagonal band of Broca (HDB) in the basal forebrain, the activity of which is thought to be correlated with animal behaving states, such as attention. Cholinergic signals in the bulb facilitate olfactory discrimination and learning, but it has remained controversial how the activity of HDB cholinergic neurons modulates neuronal excitability and olfactory responses in the MOB. In this study, we used an optogenetic approach to selectively activate HDB cholinergic neurons and recorded the effect of this activation on the spontaneous firing activity and odor-evoked responses of mouse MOB neurons. Cells were juxtacellularly labeled and their specific types were morphologically determined. We find that light stimulation of HDB cholinergic neurons inhibits the spontaneous firing activity of all major cell types, including mitral/tufted (M/T) cells, periglomerular (PG) cells, and GABAergic granule cells. Inhibitions are significantly produced by stimulation at 10 Hz and further enhanced at higher frequencies. In addition, cholinergic activation sharpens the olfactory tuning curves of a majority of M/T cells but broadly potentiates odor-evoked responses of PG cells and granule cells. These results demonstrate strong effects of the basal forebrain cholinergic system on modulating neuronal excitability in the MOB and support the hypothesis that cholinergic activity increases olfactory discrimination capability.

Postnatal and adult exposure to estradiol differentially influences adult neurogenesis in the main and accessory olfactory bulb of female mice

Neurons incorporated into the adult main olfactory bulb (MOB) and accessory olfactory bulb (AOB) derive from the subventricular zone (SVZ). Despite some recent studies on the role of olfactory neurogenesis in sociosexual behaviors mediated by hormones, data on the implication of estrogens are still lacking. Taking advantage of female aromatase-knockout (ArKO) mice, which are unable to produce estradiol across their life span, we investigated the role of estradiol exposure during early postnatal and adult periods on adult neurogenesis in the MOB and AOB. We found that proliferation of progenitor cells in the adult female SVZ was not influenced by estradiol. However, whereas adult exposure to estradiol influences the turnover of MOB newborn neurons, the survival of those in the AOB depends on exposure to estradiol during the early postnatal period. Finally, based on their expression of Zif268, we showed that newborn neurons in the MOB responded to sociosexual odors, albeit to a lesser extent in ArKO females, suggesting a contribution of estradiol during the early postnatal period to this response. Together, these results suggest that the survival and functional integration of newborn neurons in the adult female MOB and AOB are differentially influenced by estrogens from the early postnatal period to adulthood.

Cellular activation patterns of the main olfactory bulb and accessory olfactory bulb following exposure to beddings soiled by same- or opposite-sex conspecifics in Mandarin voles (Microtus mandarinus)

Using induction of Fos as an index, we studied cellular activation patterns in the accessory olfactory bulb (AOB) and main olfactory bulb (MOB) of Mandarin voles ( Microtus mandarinus (Milne-Edwards, 1871)) following their exposure to beddings soiled by different sexes. Male and female Mandarin voles that were exposed to beddings soiled by the opposite sex produced significantly more Fos-immunoreactive (Fos-ir) neurons in the anterior portion of the AOB and significantly fewer Fos-ir neurons in the posterior portion of the AOB than voles exposed to beddings soiled by the same sex. Furthermore, male and female Mandarin voles exposed to bedding soiled by different sex produced different numbers of Fos-ir cells in MOB. Mandarin voles exposed to beddings soiled by the opposite sex produced significantly more Fos-ir neurons in MOB than voles exposed to beddings soiled by the same sex. Our results establish that Mandarin voles of each sex showed different cellular activation patterns in AOB and MOB following exposures to sex-specific beddings. We suggest that both AOB and MOB were involved in sexual activities induced by chemosensory signals.

Increase in the number of tyrosine hydroxylase-containing neurons in a primary culture system of the rat accessory olfactory bulb by co-culture with vomeronasal pockets

Previously, we established a culture system of the accessory olfactory bulb in order to investigate the functional role of each accessory olfactory bulb neurons in pheromonal signal processing. In the present study, we developed a co-culture system of cultured accessory olfactory bulb neurons with partially dissociated cells of the vomeronasal organ. The dissociated cells of the vomeronasal organ form spherical structures surrounding a central cavity in culture, referred to as the vomeronasal pockets. The projection and activity of olfactory receptor neurons affect the differentiation and maturation of main olfactory bulb neurons. It was also reported induction of tyrosine hydroxylase expression in main olfactory bulb neurons when they were co-cultured with explants of the olfactory epithelium. Thus, we investigated the effects of co-culture with vomeronasal pockets on the differentiation and/or maturation of cultured accessory olfactory bulb neurons in relation to tyrosine hydroxylase expression. The number of tyrosine hydroxylase-containing neurons developmentally increased over time in the accessory olfactory bulb culture. This increase was significantly enhanced by coculture with vomeronasal pockets. Interestingly, a significant change in tyrosine hydroxylase expression was not observed when main olfactory bulb neurons were co-cultured with vomeronasal pockets. Moreover, significant changes in tyrosine hydroxylase expression were not observed when accessory olfactory bulb neurons were co-cultured with olfactory epithelium explants, as was previously observed in co-culture of main olfactory bulb neurons and olfactory epithelium explants. These results suggest that the differentiation and/or maturation of accessory olfactory bulb neurons is modified by vomeronasal organ neurons via specific interactions between the sensory organ and its target.

Age-related change of calbindin D-28k immunoreactive neurons in the rat main olfactory bulb

We examined the aged-related changes of calbindin D-28k (CB)-immunoreactive (IR) neurons in the rat main olfactory bulb (MOB). The localization of CB-IR neurons was found to be almost entirely restricted to the glomerular layer. However, a few CB-IR neurons were observed in the granular layer. Prior to the 6th postnatal month (PM 6), the CB-IR neurons had long processes, and the population of CB-IR neurons had increased significantly compared to PM 12 and 24. At PM 12, CB-IR neurons showed a tendency to be smaller and have fewer dendrites than at guess. This phenomenon became remarkable at PM 24. The distinct reduction of CB-IR dendrites in glomeruli may be due to age-related functional restrictions. Increased calcium levels in the MOB neurons may be induced to allow cytotoxic event detection in the MOB neurons, and unbalanced Ca 2+/Mg 2+-ATPase may also induce aging-related MOB morphological changes.

Habituation of odor responses in the rat anterior piriform cortex.

Simultaneous recordings of main olfactory bulb (MOB) and anterior piriform cortex (aPCX) neuron responses to repeated and prolonged odor pulses were examined in freely breathing, urethan-anesthetized rats. Comparisons of odor responses were made between multi-unit recordings of MOB activity and single-unit extracellular and intracellular recordings of Layer II/III aPCX neurons. Odor stimuli consisted of either 2-s pulses repeated at 30-s intervals or a single, prolonged 50-s stimulus. Respiration rate was monitored throughout. MOB and aPCX neuron responses to odor were quantified both through firing frequency and through the temporal patterning of firing over the respiratory cycle. The results demonstrate that aPCX neurons habituate significantly more (faster) than MOB neurons with both repeated and prolonged stimulation paradigms. This enhanced habituation is expressed as both a decrease in aPCX firing despite maintained odor-evoked MOB input and as a decrease in aPCX respiratory cycle entrainment despite maintained MOB cyclic input. Intracellular aPCX recordings suggest that several mechanisms may be involved in this experience-induced change in aPCX function, including 1) decreased excitatory driveof aPCX neurons, 2) decreased excitability of aPCX neurons,and/or 3) enhancement in odor-evoked inhibition of aPCX neurons. These studies provide the initial basis for understanding the mechanisms of nonassociative plasticity in olfactory cortex.