To study the regulation and feedback mechanism of cholecystokinin and trypsin in Black bream, Megalobrama pellegrini (Tchang, 1930) 60 days after hatching under 15 days short-term fasting and different feeding frequencies within 24 hours during the same period, Black bream (wet weight 183.75 ± 61.16 mg, total length 20.74 ± 4.08 mm) developed in a recirculating aquaculture system (RAS) were selected. In the short-term fasting trial, body weight, trypsin, and cholecystokinin (CCK) of the feeding control group (FCG) were higher than those of the fasting trial group (FTG). Trypsin and CCK in FTG reached the lowest value on day 9, and CCK content reached the highest value on day 11. In the 24-hour daily rhythm experiment, juvenile fish were randomly assigned to (A) once feeding, (B) twice feeding, (C) three times feeding, and (D) fasting. CCK showed a minimum at 1:00+ in group A, a peak at night in group B, and a maximum in group C, and a single satiety stimulus can lead to increased hunger. The four treatment groups had an apparent closed-loop regulation, while the control point of the fasting group (D) shifted forward to the next day. In this study, we found only negative feedback regulation of CCK and trypsin at the end of fasting, considering whether the secretory site or anti-inflammatory response caused the increase of CCK. The damage of epithelial cells in the villi of the foregut was greater than that in the hindgut and hepatopancreas, and the detachment of epithelial cells and the striatal margin was the main damage. Different feeding frequencies in a single day did not directly affect the long-term fluctuation of CCK and trypsin diurnal rhythm. Three meals per day may be more conducive to the long-term growth of juvenile Black bream. This study aimed to provide a reference for the feeding strategy of juvenile Black bream in the RAS.
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