Magnification In Order Research Articles

Effects of increasing molar concentration on cadmium dioxide (CdO) nanoparticles prepared by chemical Bath deposition (CBD)

Polycrystalline thin films were prepared from CdO nanoparticles in the chemical Bath deposition (CBD) with different molar concentrations at (0.01, 0.03, 0.05, 0.07) M.
 In order to obtain the best results, the structural and optical properties of CdO films were studied under the influence of increasing molar concentration, the results of the absorbance measurements there showed a change in the absorbance spectrum of the (CdO) films upon the increase in the molar concentration in the deposition, The value of the absorbance is within the limits of short wavelengths and where the absorbance increases at the wavelength (λ≤550 nm), and we note that it increases with the decrease in the wavelength and that the increase in the molar concentration in the deposition leads to an increase in thickness where the greater the absorbance increases the thickness. All samples of the prepared films were examined using scanning electron microscopy (SEM) at 2,000x magnification in order to identify the nature of the surface of the prepared films and to note the change in particle size with increasing molar concentration in the deposition. Experimental results showed that the grain size increases its crystal growth with increasing molar concentration, and this was shown by X-ray, AFM measurements

Analysis of anatomy and configuration of the canal system of the maxillary second premolar in the population of Bosnia and Herzegovina

<p>Introduction. Successful endodontic treatment depends upon the clinician's knowledge and ability to recognize and diagnose the presence of anatomical and morphological variations of the root and canal system. The aim of this study was to establish the number of roots and root canal configurations of the maxillary second premolar in the population of Bosnia and Herzegovina. Methods. The study sample was comprised of 150 maxillary second premolar teeth extracted for orthodontic or prosthetic reasons. Endodontic drills were used for trepanation of cavum dentis, and the number and patency of each root canal were determined by Kexpander # 15. Then, the samples were decalcified, made transparent and colored, to enable 3D viewing of the canal system. Decalcified teeth were observed from two projections (clinical and approximal) and analyzed in detail with a magnifying glass under 3 × and 5 × magnification in order to determine the number of roots, number of canals, root canal configuration using Vertucci's classification and number of anastomoses between canals. Statistical significance was obtained using Chi-square test. Results. The results obtained by decalcification of the teeth showed that, by radiographic analysis from the clinical projection, all the teeth had a single root. While, by the analysis from the approximal projection, 94.0% had one, 6.0% two roots. From the approximal projection, 70.7% with a single root canal and 29.3% with two root canals are visualized. The most common type of root canal configuration in the maxillary second premolars was Type I in both clinical (87.9%) and approximal projection (40.7%). Conclusion. These results emphasize the importance of knowing the variations in root canal morphology, because excluding the possibility of morphological variations can lead to failure of endodontic therapy</p>

Compare the forecasting method of artificial neural network and support vector regression model to measure the bullwhip effect in supply chain

The objective of this study was to compare the Bullwhip Effect (BWE) in the supply chain through two methods and to determine the inventory policy for the uncertainty demand. It would be useful to determine the best forecasting method to predict the certain condition. The two methods are Artificial Neural Network (ANN) and Support Vector Regression (SVR), which would be applied in this study. The data was obtained from the instant noodle dataset where it was in random normal distribution. The forecasting demands signal have Mean Squared Error (MSE) where it is used to measure the bullwhip effect in the supply chain member. The magnification of order among the member of the supply chain would influence the inventory. It is quite important to understand forecasting techniques and the bullwhip effect for the warehouse manager to manage the inventory in the warehouse, especially in probabilistic demand of the customer. This process determines the appropriate inventory policy for the retailer. The result from this study shows that ANN and SVR have the variance of 0.00491 and 0.07703, the MSE was 1.55e-6 and 1.53e-2, and the total BWE was 95.61 and 1237.19 respectively. It concluded that the ANN has a smaller variance than SVR, therefore, the ANN has a better performance than SVR, and the ANN has smaller BWE than SVR. At last, the inventory policy was determined with the continuous review policy for the uncertainty demand in the supply chain member.

Effect of sonic versus ultrasonic activation on aqueous solution penetration in root canal dentin.

The aim of this study was to observe the penetration of an aqueous solution into the root canal dentin under sonic activation and ultrasonic activation. Materials and Method: This study consisted of experimental in vitro research. In order to achieve a closed system, the apex of 45 single-rooted teeth was sealed with wax. The step-back technique was manually performed using a K50 apical master file and 3 groups were organized according to the protocol of the final irrigant activation: Group I: non-activated Chinese ink for 30 seconds, Group II: Chinese ink sonically activated with EndoActivator for 30 seconds, and Group III: Chinese ink ultrasonically activated with Varios 350 equipment for 30 seconds. Teeth were sectioned longitudinally, and the samples obtained were observed under a stereomicroscope at 1X magnification in order to be photographed and scanned to calculate the penetration area using the Image J software. The tinted radicular area was evaluated in relation to the total area of the root dentin. The Tukey’s post-hoc test and ANOVA were used for the statistical analysis (p<0.05). Results: Group I and II obtained 9.13% and 9.42% penetration respectively, while in group III the highest degree of dye infiltration was achieved (13.9%), being statistically significant (p<0.001). Conclusions: Ultrasonic activation produced a significantly higher penetration of the dye when compared to conventional activation and sonic activation.

Magnification of digital hip radiographs differs between clinical workplaces

Preoperative planning for total hip arthroplasty includes templating on anteroposterior radiographs. It is necessary to consider radiographic magnification in order to scale templates accurately. Studies dealing with hip templating report different values of radiographic magnification. It is not clear if the observed difference in magnification between the studies is caused by variability in studied groups, methodology or instrumentation. We hypothesize that there is a difference in magnification between clinical workplaces. Within this study, radiographic magnification was estimated on 337 radiographs of patients after total hip surgery from five orthopaedic departments in the Czech Republic. Magnification was determined for each patient as a ratio between diameter of implanted femoral head measured on radiogram and its true size. One-way ANOVA revealed significant differences in magnification between workplaces (F(4,332) = 132, p≤0.001). These results suggest that radiographic magnification depends on the workplace where it is taken or more precisely on radiographic device. It indicates potential limits in generalizability of results of studies dealing with preoperative planning accuracy to other institutions.

Early markers of myocardial ischemia: from the experimental model to forensic pathology cases of sudden cardiac death.

The goal of this study was to assess whether early markers of myocardial ischemia, identified in a previous experimental work, can be applied in forensic pathology cases of sudden, ischemic cardiac death. These markers include desphosphorylated connexin 43 (Cx43), JunB, TUNEL assay, myoglobin, and troponin T. Fourteen cases of sudden cardiac death with gross and/or histological signs of myocardial infarction and 14 cases of sudden cardiac death with signs of early ischemia at histology and positive immunoreactions for fibronectin and C5b-9 were investigated. The control group was represented by 15 hanging (global hypoxia) cases. Immunohistochemical reactions were classified into four degrees and compared among groups. Cx43 and JunB were significantly more expressed in hanging than in ischemia/infarction, but they showed a different distribution in the tissue (sub-endocardial in ischemia/infarction, diffuse in hanging) and a different intensity of the signal. TUNEL assay was significantly more expressed in the group of early ischemia than in myocardial infarction. Myoglobin and troponin T did not show any significantly different expression among the three groups. Depletion markers have a limited application in forensic cases, and this is mostly because positive (depleted) areas are difficult to distinguish from artifactually paler areas. Nuclear markers (JunB and TUNEL), on the other hand, require a well-trained eye and a high magnification in order to be distinguished. Cx43, JunB, and TUNEL assays were confirmed to be early, sensitive markers for myocardial ischemia. Nonetheless, they are not specific, as they are expressed in global hypoxia as well, but with a different tissular distribution.

Optical method for automated measurement of glass micropipette tip geometry

Many experimental biological techniques utilize hollow glass needles called micropipettes to perform fluid extraction, cell manipulation, and electrophysiological recordings. For electrophysiological recordings, micropipettes are typically fabricated immediately before use using a “pipette puller”, which uses open-loop control to heat a hollow glass capillary while applying a tensile load. Variability between manufactured micropipettes requires a highly trained operator to qualitatively inspect each micropipette; typically this is achieved by viewing the pipette under 40–100× magnification in order to ensure that the tip has the correct shape (e.g., outer diameter, cone angle, taper length). Since laboratories may use hundreds of micropipettes per week, significant time demands are associated with micropipette inspection. Here, we have automated the measurement of micropipette tip outer diameter and cone angle using optical microscopy. The process features repeatable constraint of the micropipette, quickly and automatically moving the micropipette to bring its tip into the field of view, focusing on the tip, and computing tip outer diameter and cone angle measurements from the acquired images by applying a series of image processing algorithms. As implemented on a custom automated microscope, these methods achieved, with 95% confidence, ±0.38μm repeatability in outer diameter measurement and ±5.45° repeatability in cone angle measurement, comparable to a trained human operator. Accuracy was evaluated by comparing optical pipette measurements with measurements obtained using scanning electron microscopy (SEM); optical outer diameter measurements differed from SEM by 0.35±0.36μm and optical cone angle measurements differed from SEM by −0.23±2.32°. The algorithms we developed are adaptable to most commercial automated microscopes and provide a skill-free route to rapid, quantitative measurement of pipette tip geometry with high resolution, accuracy, and repeatability. Further, these methods are an important step toward a closed-loop, fully-automated micropipette fabrication system.

Design and fabrication of a miniature objective consisting of high refractive index zinc sulfide lenses for laser surgery.

A miniature laser ablation probe relying on an optical fiber to deliver light requires a high coupling efficiency objective with sufficient magnification in order to provide adequate power and field for surgery. A diffraction-limited optical design is presented that utilizes high refractive index zinc sulfide to meet specifications while reducing the miniature objective down to two lenses. The design has a hypercentric conjugate plane on the fiber side and is telecentric on the tissue end. Two versions of the objective were built on a diamond lathe-a traditional cylindrical design and a custom-tapered mount. Both received an antireflective coating. The objectives performed as designed in terms of observable resolution and field of view as measured by imaging a 1951 USAF resolution target. The slanted edge technique was used to find Strehl ratios of 0.75 and 0.78, respectively, indicating nearly diffraction-limited performance. Finally, preliminary ablation experiments indicated threshold fluence of gold film was comparable to similar reported probes.

Influence of the rotary and/or oscillatory reciprocating systems in the morphological changes of narrow and curved molar root canals anatomy

OBJECTIVE: This study evaluated four mechanized Endodontic systems, ProTaper Universal, K3 Endo, Twisted file (rotary) and the oscillatory reciprocating system Endo - Eze TiLOS, in order to verify and measure alterations in original anatomy with deviations at cervical, medium and apical root canal thirds. MATERIAL AND METHOD: It was used MB root canals of 60 extract human lower molars, to produce a line of severe angles, according to the classification of Schneider. Samples were included in Ester vinyl resin, mounted in the Teflon Furnace, transversally sectioned at the cervical, middle and apical thirds, which were subsequently photographed using a digital camera Cyber Shot DSC-TX10,attached to an operating microscope 3101XY DFVasconcelos with 40× magnification in order to measure the anatomical transversal area of the root canal, using the software AutoCAD 2008, comparing pre and post-instrumentation. All samples assembled in the Furnace also were submitted to x-ray in a standardized way to enable the comparison of the angle of Schneider pre-and post-instrumentation. Once collected the data, they were compared statistically using the program BioEstat 5.0. RESULT: The analysis of the results showed that in the cervical third, rotary systems were more effective than Endo-Eze TiLOS System with statistically significant differences (p ≤ 0,05). Apical and middle third changes in anatomy were similar, but apically, the ProTaper system caused more deviations when comparing the angle of Schneider,and areas before and after instrumentation(p ≤ 0,05). CONCLUSION: It was concluded that all the systems caused alteration in the original anatomy of the root canal when parameters as angle of Schneider and areas before and after preparation were used.

Supraclavicular artery island flap innervation: Anatomical studies and clinical implications

Recently, the supraclavicular artery island flap has gained popularity as a regional flap for head and neck reconstruction. During clinical follow-up, some patients report referred sensation to the shoulder when there is contact with the flap skin island surface. The authors examine the anatomical origin/characteristics of the supraclavicular nerves (C3-4) to this flap and its relationship to the flap pedicle and anatomical boundaries. SAI flap harvest and nerve dissection was performed in seven fresh frozen cadavers (n = 10) using loupe magnification in order to further elucidate the sensory nerve branches in a typical SAI flap. Branches of the supraclavicular nerve innervating the SAI flap were found to emerge from the deep fascia at a separate location from the vascular pedicle with the major nerve root exiting underneath the sternocleidomastoid muscle near the midpoint of the muscle belly. The nerve branches proximal to the pedicle with one branch exiting anterior to the flap and another running axially along the length of the flap. The majority (9/10) flaps had a major cutaneous nerves located 1-2 cm anterior to the pedicle. One (1/10) of the flaps had a major cutaneous nerve located 1-2 cm posterior to the pedicle toward the trapezius muscle. In 3 of the 10 flaps, smaller cutaneous nerves were also found posterior to the pedicle in a more distal location of the flap. The supraclavicular nerves innervating the SAI flap are easily identifiable and can be preserved or ligated, depending on the desired flap function, when present close to the pedicle. Further clinical investigation is warranted to confirm the potential benefit of using the SAI flap as a neurotized regional flap for head/neck reconstruction.

Differentiation of normal skin and melanoma using high resolution hyperspectral imaging

We investigated the use of high resolution hyperspectral imaging microscopy to detect abnormalities in skin tissue using hematoxylin-eosin stained preparations of normal and abnormal skin, benign nevi and melanomas. A goal of this study was to provide objective data that could be utilized by any researcher; and form the beginnings of a reference spectral data base. All spectral characterizations were acquired in percent transmission, and absorption, with contiguous wavelength acquisition between 400 and 800 nm; and a spectral resolution of ~1 nm. Biopsy sections were characterized with varying sample thickness, staining and magnification in order to determine their impact on spectral characterizations. Spectra were classified using spectral waveform cross correlation analysis, an algorithm that is linearity invariant. Classified spectra were incorporated into spectral libraries; and all spectra acquired from the field of view were correlated with library spectra to a quantified, user-determined, confidence threshold (minimum correlation coefficient). The results revealed that all skin conditions in our initial data sets could be objectively differentiated providing that staining and section thickness was controlled. We also demonstrated that it is likely that a reference spectral library database could be created to include bioinformatics and cluster analysis. This would assist multiple laboratories to participate in the input and retrieval of target spectral information.

Paleocene coccoliths and coccospheres in deposits of the Maastrichtian stage at the `type locality' and type area in SE Limburg, The Netherlands

Three so-called `Tertiary Newcomer Coccoliths'— Cruciplacolithus primus, Neobiscutum parvulum and Neobiscutum romeinii—were found in what are generally believed to be upper Maastrichtian deposits at the ENCI quarry, St. Pietersberg near Maastricht, the CURFS quarry near Houthem and from the Maastrichtian part of the K/T boundary section of the cave system at Geulhemmerberg near Valkenburg, southeastern Limburg, The Netherlands. The mentioned species appear already far below The Berg en Terblijt Horizon at the CURFS quarry and Geulhemmerberg, which is actually supposed to mark the K/T boundary, at the ENCI quarry they range throughout the Maastricht Formation above the Horizon of Lichtenberg. The specimens are well preserved and originate from undisturbed sediment layers, thus it seems that they were not transported into the Maastrichtian sediments by water circulation or burrowing effects. The state of preservation and the presence of intact coccospheres indicate that they are autochthonous. C. primus, N. romeinii and N. parvulum are extremely small in size (800 nm to 2.0 μm), implying that a scanning electron microscope (SEM) must be set at least to 3 K magnification in order to detect them. With a light microscope (LM) at 1 K they cannot be classified, which is probably the reason why previous investigators who studied the same sections based on LM did not mention these species from the upper Maastrichtian. They furthermore appear in very low numbers contributing to around 1% of an associated calcareous nannoflora which is equivalent to the assemblage of the CC26 Nephrolithus frequens Zone of Cepek and Hay (1969). The biostratigraphic setting of the ENCI quarry, CURFS quarry and Geulhemmerberg have been the subject of a long and controversial discussion whether the exposed sediments still belong to the upper Maastrichtian or represent the lower Tertiary. Reworking of macrofossils and microfossils or autochthony of index fossils had been discussed and used to support the different opinions about the chronostratigraphy of these sections. Up to now there has not been any contribution which might lead to convincing data. If C. primus, N. romeinii, and N. parvulum are indeed `Tertiary Newcomer Coccoliths', the sediments exposed above the Horizon of Lichtenberg at the ENCI quarry, and the sediments below The Berg en Terblijt Horizon studied at CURFS quarry and Geulhemmerberg do belong to the NP 1 Biantholithus sparsus Zone, which means they are of Early Paleocene age. However, if these coccoliths already originate in the late Maastrichtian, the definition of the K/T boundary by calcareous nannoflora must be revised.

Structure of frozen hydrated deoxy-sickle hemoglobin fibers

Three-dimensional reconstruction of a helical particle can be accomplished from a single lateral view, but in practice the reconstruction procedure must be optimized to make the best use of the available projection data. This is especially true for unstained frozen hydrated (ice embedded) specimens, whose micrographs have a characteristically low signal to noise ratio. Deoxy-sickle hemoglobin (HbS) fibers present an additional difficulty as well because these particles have a variable pitch and may also have variation in their unit cell structure. We have carried out a three-dimensional reconstruction of an unstained frozen hydrated HbS fiber (Figs. 1 & 2) by convolution back projection. The procedures used to facilitate this three-dimensional reconstruction are described below.In this work especially close attention was paid to determining the image magnification in order that the reconstruction of this HbS fiber could be compared with previous HbS fiber models (Table 1) which are based on reconstructions of negatively stained HbS fibers. In order to determine the HbS fiber's dimensions accurately the particle (Fig. 1a) was reconstructed twice.

Energy partition in the 1982 eruption of El Chichon volcano, Chiapas, Mexico

The 1982 eruption of El Chichon volcano, Chiapas, Mexico has been extensively investigated from various standpoints, including seismicity, tephra geology, atmospheric effects and tectonic structure. In this paper, the six largest of a series of explosions are isolated, considering various sources of information. Then, the seismic energy released by volcanic earthquakes and tremor is estimated using seismograms of low magnification in order to avoid saturation of seismic wave amplitudes. The explosion kinetic energy is evaluated by two methods: one by the initial velocity of the ejecta and the other by the air wave data recorded at a distance. Both the results are roughly coincident. The thermal energy is classified into two components: that transferred by volcanic ejecta and that consumed by convective clouds. The thermal energy released by the explosions is very large compared with that of mechanical energy, such as the seismic energy and explosion kinetic energy. After the last major explosion of April 4, A-type volcanic earthquakes swarmed till the end of April. Their occurrence rate decreased with time in an aftershock-like sequence. These earthquake swarms may have been due to post-eruptive readjustment of the stress field within and near the volcano. Such seismic activity contrasts with other examples of similar major eruptions, which were followed by growth of lava domes in the new craters after the paroxysmal eruptions. The 1982 eruption of El Chichon volcano has not been followed by the growth of a lava dome within the newly formed crater.

MADEIRAS DA AMAZÔNIA QUE APRESENTAM RAIOS LARGOS

O presente trabalho visa o estudo anatômico macroscópico de 80 espécies lenhosas da Amazônia representadas por 19 Famílias e 46 Gêneroe que apresentam raios considerados largos, segundo adaptação feita pela Divisão de Anatomia e Identificação de Madeiras/INPA, tomando como base a Norma Técnica COPANT (1974). Para cada espécie constam as seguintes informações: caracteres gerais, descrição macroscópica, usos comune, nomee vulgares, um atlas de fotomacrografias com 10x de aumento para auxiliar na identificação dae espécies, um gráfico comparativo contendo a classificação da largura doe raios estudados entre a Norma Técnica COPANT (1974) e a DAIM (1987), um histograma com a frequência da massa específica das espécies, uma listagem contendo as espécies estudadas com a classificação da massa específica, um índice alfabético por famílias e outro por nomee vulgares, ainda um glossário dos termos técnicos utilizados na descrição macroecópica de madeiras acompanhado de um desenho esquemático de um corpo de prova.

3000 times grating compressor with positive group velocity dispersion: Application to fiber compensation in 1.3-1.6 µm region

A compressor is designed that presents an opposite sign of the dispersion to that of standard two grating compressors. This is achieved by placing a telescope between gratings in order to modify in an adequate manner the phase shift for different wavelengths. The telescope simultaneously provides a high magnification in order to compensate more than 90 km of standard monomode fibers in the 1.6 μm region, yielding compression factors as high as 3000. Analytical expressions for Gaussian beams are found and limitations due to lateral spectral walkoff and telescope pupils are discussed.

Microsurgical anatomy of the pineal region.

Thirty cadaver brains were examined under X 6 to 16 magnification in order to define the microsurgical anatomy of the pineal region, particularly the relationship of the pineal body, posterior cerebral artery, superior cerebellar artery, vein of Galen, basal vein of Rosenthal, internal cerebral vein, straight sinus, bridging vein, the size of the tentorial notch, and the third and the fourth cranial nerves. The infratentorial and supratentorial approaches to the pineal region are compared from the viewpoint of microsurgical anatomy.

AN ADJUSTABLE TELESCOPIC OTOSCOPE

The older otologists, as is evidenced by their application of the lens system to the ear specula, appreciated the need of magnification in order to be better able to study the tympanic membrane and its varied pathologic manifestations. This Adjustable telescopic otoscope. same need was obvious at a later date with the introduction of the electrically illuminated otoscopes, which were almost always supplied with a lens system for magnification. The new electrically illuminated device with means of magnification has become very popular, especially with the general practitioner and the pediatrician. However, one drawback to its use is apparent: It has a lens system with a fixed focus. As is well known, the external auditory meatus varies in depth, depending on a number of factors, the most important being that of the age of the patient. A sharp image of the tympanic membrane could be obtained in the fixed focus