Magnetic Resonance Measurements Research Articles

The cardiovascular exercise response in children with overweight or obesity children measured by cardiovascular magnetic resonance imaging.

Overweight and obesity are among the main causes of cardiovascular diseases. Exercise testing can aid in the early detection of subtle cardiac dysfunction not present in rest. We hypothesized that the cardiovascular response to exercise is impaired among children with overweight or obesity, characterized by the inability of the cardiovascular system to adapt to exercise by increasing cardiac volumes and blood pressure. We performed a cardiovascular stress test to investigate whether the cardiovascular exercise response is altered in children with overweight and obesity, as compared to children with a normal weight. A subgroup of the Generation R population-based prospective cohort study, consisting of 41 children with overweight or obesity and 166 children with a normal weight with a mean age of 16 years, performed an isometric exercise. Continuous heart rate and blood pressure were measured during rest, exercise and recovery. Cardiovascular magnetic resonance (CMR) measurements were performed during rest and exercise. Higher BMI was associated with a higher resting systolic and diastolic blood pressure (difference: 0.24 SDS (95% CI 0.10, 0.37) and 0.20 SDS (95% CI 0.06, 0.33)) and lower systolic and diastolic blood pressure increases from rest to peak exercise (-0.11 SDS (95% CI -0.20, -0.03) and -0.07 SDS (95% CI -0.07, -0.01)). BMI was also associated with a slower decrease in systolic and diastolic blood pressure during recovery (p values < 0.05). Higher childhood BMI was associated with lower BSA corrected left ventricular mass, end-diastolic volume and stroke volume (p values < 0.05). There were no associations of childhood BMI with the cardiac response to exercise measured by heart rate and CMR measurements. Childhood BMI is, across the full range, associated with a blunted blood pressure response to static exercise but there were no differences in cardiac response to exercise. Our findings suggest that adiposity may especially affect the vascular exercise reaction without affecting cardiac response.

Design of a High-Homogeneity 55 T Pulsed Magnet for High-Resolution Nuclear Magnetic Resonance Measurements

Design of a High-Homogeneity 55 T Pulsed Magnet for High-Resolution Nuclear Magnetic Resonance Measurements

In Situ Characterization of Metastable Pb3O5 and Pb2O3 Phases During Thermal Decomposition of PbO2 to PbO.

Nonstoichiometric lead oxides play a key role in the formation and cycling of the positive electrodes in a lead acid battery. These phases have been linked to the underutilization of the positive active material but also play a key role in the battery's cycle life, providing interparticle adhesion and the connection to the underlying lead grid. Similar phases have previously been identified by mass loss or color change during thermal annealing of PbO2 to PbO, suggesting that at least two intermediate PbOx phases exist. Using multiple, in situ analysis techniques (powder diffraction, X-ray absorption, X-ray photoelectron spectroscopy) and ex situ nuclear magnetic resonance measurements, the structural conversion and changes in the lead oxidation state were identified during this process. Isolation of the PbOx phases enabled confirmation of Pb3O5 and Pb2O3 by diffraction and the first 207Pb NMR measurement of these intermediates. The thermodynamic and kinetic stability of these intermediates and other reported polymorphs were determined by density functional theory, providing key insight into their origins and variation of PbOx structures found in previous studies.

Study of the preparation, characterization, and solubility of lidocaine complexed with 5-sulfosalicylic acid dihydrate

Objective: This study was to prepare solid dispersions of lidocaine (Lid) with 5-sulfosalicylic acid dihydrate (SSA) by freeze-drying (freeze-dried [FD] Lid/SSA = 1/1) and to evaluate their physical properties. Methods: Here, we evaluated the physicochemical properties and solubility of solid dispersions of lidocaine (Lid) and 5-sulfosalicylic acid dihydrate (SSA) prepared by freeze-drying (freeze-dried [FD] Lid/SSA = 1/1). Results: Differential scanning calorimetry measurements showed that after freeze-drying, the endothermic peak due to Lid melting, the dehydration peak, and the endothermic peak due to SSA melting disappeared. Powder X-ray diffraction results showed that the characteristic Lid and SSA peaks disappeared after freeze-drying, indicating a halo pattern. The near-infrared spectroscopy results suggested that Lid-derived -NH and -CH groups and the Lid-derived -OH and -CH groups from the SSA peak shifted and broadened after freeze-drying, suggesting their involvement in complex formation through Lid/SSA intermolecular interactions. Nuclear Overhauser effect spectroscopy-nuclear magnetic resonance (NMR) measurements showed a cross-peak due to the interaction between the Lid-derived -CH group and the SSA-derived -OH group, suggesting hydrogen bonding. Diffusion-ordered spectroscopy NMR measurements showed that the diffusion coefficients of Lid and SSA aggregated in FD Lid/SSA, suggesting a change in Lid dispersibility in the solvent owing to the formation of a complex with SSA. The solubility of FD Lid/SSA was approximately 88 mg/mL (∼20-fold higher than that of Lid). Conclusions: These findings suggest that complex formation occurred in FD Lid/SSA; this enhanced the solubility of this dispersion.

Calcium ions do not influence the Aβ(25–35) triggered morphological changes of lipid membranes

We have studied the effect of calcium ions (Ca2+) at various concentrations on the structure of lipid vesicles in the presence of amyloid-beta peptide Aβ(25–35). In particular, we have investigated the influence of calcium ions on the formation of recently documented bicelle-like structures (BLSs) emerged as a result of Aβ(25–35) triggered membrane disintegration. First, we have shown by using small-angle X-ray and neutron scattering that peptide molecules rigidify the lipid bilayer of gel phase DPPC unilamellar vesicles (ULVs), while addition of the calcium ions to the system hinders this effect of Aβ(25–35). Secondly, the Aβ(25–35) demonstrates a critical peptide concentration at which the BLSs reorganize from ULVs due to heating and cooling the samples through the lipid main phase transition temperature (Tm). However, addition of calcium ions does not affect noticeably the Aβ-induced formation of BLSs and their structural parameters, though the changes in peptide's secondary structure, e.g. the increased α-helix fraction, has been registered by circular dichroism spectroscopy. Finally, according to 31P nuclear magnetic resonance (NMR) measurements, calcium ions do not affect the lipid-peptide arrangement in BLSs and their ability to align in the magnetic field of NMR spectrometer. The influences of various concentrations of calcium ions on the lipid-peptide interactions may prove biologically important because their local concentrations vary widely in in-vivo conditions. In the present work, calcium ions were investigated as a possible tool aimed at regulating the lipid-peptide interactions that demonstrated the disruptive effect of Aβ(25–35) on lipid membranes.

Evaluation of pore characteristics evolution and damage mechanism of granite under thermal-cooling cycle based on nuclear magnetic resonance technology

During the development process of deep geothermal energy (Hot dry rock, HDR), the high-temperature granite matrix in the wellbore needs to withstand thermal-cooling cycles, easily leading to wellbore instability and collapse. Considering the actual drilling engineering using different types of drilling fluid, thermal-cooling cycle experiments (cycles of 1, 2, 4, 8, 12 times) of granite were conducted using natural, water, and liquid nitrogen (LN2) cooling methods. Based on the low-field nuclear magnetic resonance measurement, the development and evolution of pore size, quantity, and distribution under different cooling ways and thermal cycles were studied, and the changes in rock mechanical parameters under different cooling methods and thermal cycling conditions were quantified. Then, the rock damage coefficients based on the variation patterns of different types of pores were calculated, and a quantitative relationship was established among T2 spectrum, pore, and mechanical damage. Finally, the proton weighting method was used to analyze the changes in the internal bearing area of rocks, revealing the deterioration mechanism of rock mechanical properties under different cooling ways and thermal cycling conditions. The results showed that the porosity increased with thermal cycles during the thermal-cooling cycle treatment, and the growth rate was first fast and then slow. Under the same number of thermal cycles, the groundwater cooling porosity was the highest, followed by LN2 and natural cooling. The relationship between T2 spectral area and rock damage was established on this basis, and the rock damage value increased with thermal cycles in an exponential function, and the proportion of large pores significantly affected the pore damage. A relationship (positively proportional linear) between pore damage and the mechanical degradation coefficient of high-temperature cooling cycle granite and a method for predicting rock degradation through pore damage were established, the deterioration mechanism of rock mechanical properties under thermal cycle treatment was revealed.

Accelerated cardiac cine magnetic resonance imaging using deep low-rank plus sparse network: validation in patients.

Accurate and reproducible assessment of left ventricular (LV) volumes is important in managing various cardiac conditions. However, patients are required to hold their breath multiple times during data acquisition, which may result in discomfort and restrict cardiac motion, potentially compromising the accuracy of the detected results. Accelerated imaging techniques can help reduce the number of breath holds needed, potentially improving patient comfort and the reliability of the LV assessment. This study aimed to prospectively evaluate the feasibility and accuracy of LV assessment with a model-based low-rank plus sparse network (L+S-Net) for accelerated magnetic resonance (MR) cine imaging. Fourty-one patients with different cardiac conditions were recruited in this study. Both accelerated MR cine imaging with L+S-Net and traditional electrocardiogram (ECG)-gated segmented cine were performed for each patient. Subjective image quality (IQ) score and quantitative LV volume function parameters were measured and compared between L+S-Net and traditional standards. The IQ score and LV volume measurements of cardiovascular magnetic resonance (CMR) images reconstructed by L+S-Net and standard cine were compared by paired t-test. The acquisition time of the two methods was also calculated. In a quantitative analysis, L+S-Net and standard cine yielded similar measurements for all parameters of LV function (ejection fraction: 35±22 for standard vs. 33±23 for L+S-Net), although L+S-Net had slightly lower IQ scores than standard cine CMR (4.2±0.5 for L+S-Net vs. 4.8±0.4 for standard cine; P<0.001). The mean acquisition time of L+S-Net and standard cine was 0.83±0.08 vs. 6.35±0.78 s per slice (P<0.001). Assessment of LV function with L+S-Net at 3.0 T yields comparable results to the reference standard, albeit with a reduced acquisition time. This feature enhances the clinical applicability of the L+S-Net approach, helping alleviate patient discomfort and motion artifacts that may arise due to prolonged acquisition time.

Non-invasive mapping of brown adipose tissue activity with magnetic resonance imaging

Thermogenic brown adipose tissue (BAT) has a positive impact on whole-body metabolism. However, in vivo mapping of BAT activity typically relies on techniques involving ionizing radiation, such as [18F]fluorodeoxyglucose ([18F]FDG) positron emission tomography (PET) and computed tomography (CT). Here we report a noninvasive metabolic magnetic resonance imaging (MRI) approach based on creatine chemical exchange saturation transfer (Cr-CEST) contrast to assess in vivo BAT activity in rodents and humans. In male rats, a single dose of the β3-adrenoceptor agonist (CL 316,243) or norepinephrine, as well as cold exposure, triggered a robust elevation of the Cr-CEST MRI signal, which was consistent with the [18F]FDG PET and CT data and 1H nuclear magnetic resonance measurements of creatine concentration in BAT. We further show that Cr-CEST MRI detects cold-stimulated BAT activation in humans (both males and females) using a 3T clinical scanner, with data-matching results from [18F]FDG PET and CT measurements. This study establishes Cr-CEST MRI as a promising noninvasive and radiation-free approach for in vivo mapping of BAT activity.

Diffusion Nuclear Magnetic Resonance Measurements on Cationic Gold (I) Complexes in Catalytic Conditions: Counterion and Solvent Effects.

The amount of free ions, ion pairs, and higher aggregate of the possible species present in a solution during the gold(I)-catalyzed alkoxylation of unsaturated hydrocarbon, i.e., ISIP (inner sphere ion pair) [(NHC)AuX] and OSIP (outer sphere ion pairs) [(NHC)Au(TME)X] [NHC 1,3-bis(2,6-di-isopropylphenyl)-imidazol-2-ylidene; TME = tetramethylethylene (2,3-bis methyl-butene); X- = Cl-, BF4-, OTf-; and OTs- BArF4- (ArF = 3,5-(CF3)2C6H3)], has been determined. The 1H and 19F DOSY NMR measurements conducted in catalytic conditions indicate that the dissociation degree (α) of the equilibrium ion pair/free ions {[(NHC)Au(TME)X] [(NHC)Au(TME)]+ + X-} depends on the nature of the counterion (X-) when chloroform is the catalytic solvent: while the compounds containing OTs- and OTf- as the counterion gave a low α (which means a high number of ion pairs) of 0.13 and 0.24, respectively, the compounds containing BF4- and BArF4- showed higher α values of 0.36 and 0.32, respectively. These results experimentally confirm previous deductions based on catalytic and theoretical data: the lower the α value, the greater the catalytic activity because the anion that can activate methanol during a nucleophilic attack, although the lower propensity to activate methanol of BF4- and BArF4-, as suggested by the DFT calculations, cannot be completely overlooked. As for the effect of the solvent, α increases as the dielectric constant increases, as expected, and in particular, green solvents with high dielectric constants show a very high α (0.90, 0.84, 0.80, and 0.70 for propylene carbonate, γ-valerolactone, acetone, and methanol, respectively), thus confirming that the moderately high activity of NHC-Au-OTf in these solvents is due to the specific effect of polar functionalities (O-H, C=O, O-R) in activating methanol. Finally, the DOSY measurements conducted in p-Cymene show the formation of quadrupole species: under these conditions, the anion can better exercise its 'template' and 'activating' roles, giving the highest TOF.

Emergence of flat bands and ferromagnetic fluctuations via orbital-selective electron correlations in Mn-based kagome metal

Kagome lattice has been actively studied for the possible realization of frustration-induced two-dimensional flat bands and a number of correlation-induced phases. Currently, the search for kagome systems with a nearly dispersionless flat band close to the Fermi level is ongoing. Here, by combining theoretical and experimental tools, we present Sc3Mn3Al7Si5 as a novel realization of correlation-induced almost-flat bands in the kagome lattice in the vicinity of the Fermi level. Our magnetic susceptibility, 27Al nuclear magnetic resonance, transport, and optical conductivity measurements provide signatures of a correlated metallic phase with tantalizing ferromagnetic instability. Our dynamical mean-field calculations suggest that such ferromagnetic instability observed originates from the formation of nearly flat dispersions close to the Fermi level, where electron correlations induce strong orbital-selective renormalization and manifestation of the kagome-frustrated bands. In addition, a significant negative magnetoresistance signal is observed, which can be attributed to the suppression of flat-band-induced ferromagnetic fluctuation, which further supports the formation of flat bands in this compound. These findings broaden a new prospect to harness correlated topological phases via multiorbital correlations in 3d-based kagome systems.

Experiment study on particle pulverization-assisted convective air drying of sewage sludge

Sewage sludge drying is a pivotal step in sludge reduction, harmlessness, and resource utilization. Pulverizing sewage sludge can significantly increase the surface area of sludge particles available for contact with the air medium, consequently strengthening the convective drying process of the sludge. In this paper, the adopted deep drying system mainly combined sewage sludge pulverization and sewage sludge drying functions. Parametric studies were carried out to investigate the effects on the drying process. The moisture content of the sewage sludge decreased from 50.3% to 26.8% when the linear speed of the rotating blade tip of the pulverizing dryer and induced draft fan reached 28.3 m·s−1, 62.8 m−1, and air velocity reached 9.5 m·s−1, respectively. Furthermore, this work also preliminarily reveals the mechanism of the pulverizing dryer. The pores on the sludge particle surfaces are destroyed during the drying process. As the linear speed of the rotating blade tip in the pulverizing dryer increased, the hydrophilicity and the average particle size of the sewage sludge particles decreased, with the particle size representing the highest volume fraction being reduced by 62% in the particle size distribution. The pulverizing dryer not only breaks up the sludge to increase the surface area of the sludge in contact with the air but may also have a centrifugal effect. As the intensity of the pulverizing dryer increases, Nuclear Magnetic Resonance (NMR) measurements show a leftward shift in the peak of the weakly bound water in the sludge and a noticeable shortening of the transverse relaxation time. Overall, the results suggested that pulverizing is a suitable assistive method to improve the drying effect of the sewage sludge.

Characterization of brittleness index of gas shale and its influence on favorable block exploitation in southwest China

The microstructure, mineral composition, total organic carbon content, etc., of gas shale are crucial parameters for shale reservoirs, which can directly/indirectly affect shale brittleness, fracturing effect, adsorption ability and production efficiency. The study proposed a workflow to characterize the physical and mechanical parameters of Lower Silurian Longmaxi shale outcrop samples extracted from the favorable block in Changning, Sichuan, southwest China. This study elaborated on the influence of these physical and mechanical characteristics and proposed a corresponding brittleness index on shale extraction. In addition, it put forward corresponding suggestions for development and risk control. For a better understanding the mechanisms of shale gas storage and production, XRD, XRF, SEM, low temperature Nitrogen adsorption method, nuclear magnetic resonance and other measurements were employed to analyze and study the mineral composition, microstructure, and adsorption performance of shale. The results demonstrated that the pores of shale are mainly slit pores; there are diverse pore types in shale, mainly including intergranular pores, mineral particle dissolution pores, and internal pores of organic matter; The samples with relatively low porosity also noticeably exhibit ultra-low permeability, and the nanopore structure is remarkably significant, with distribution primarily in range of 5–237 nm. Finally, a brittleness index considering the influence of water content and the mechanical properties was proposed, and the coupling interaction of various minerals components and mechanical properties on the brittleness index can more objectively reflect the brittleness characteristics of deep shale formation.

Nuclear magnetic resonance studies in a model transverse field Ising system

The suppression of ferroquadrupolar order in TmVO4 in a magnetic field is well-described by the transverse field Ising model, enabling detailed studies of critical dynamics near the quantum phase transition. We describe nuclear magnetic resonance measurements in pure and Y-doped single crystals. The non-Kramers nature of the ground state doublet leads to a unique form of the hyperfine coupling that exclusively probes the transverse field susceptibility. Our results show that this quantity diverges at the critical field, in contrast to the mean-field prediction. Furthermore, we find evidence for quantum critical fluctuations present near Tm-rich regions in Y-doped crystals at levels beyond which long-range order is suppressed, suggesting the presence of quantum Griffiths phases.

Elucidating Consequences of Selenium Crystallinity on Its Electrochemical Reduction in Aluminum-Selenium Batteries.

Selenium (Se) is an attractive positive electrode material for rechargeable aluminum (Al) batteries due to its high theoretical capacity of 2037 mA h g-1 and its higher electronic conductivity compared to sulfur. Selenium can undergo a series of electrochemical reactions between Se(-II) and Se(IV), resulting in a six-electron capacity per Se atom. However, existing Al-Se battery literature is inconsistent regarding the different electrochemical reactions possible, while the conditions enabling the electrochemical reduction of Se to Al2Se3 are not well understood. Here, we demonstrate that this electrochemical reduction is achievable using amorphous selenium but is suppressed for crystalline selenium. We further show that the electrochemical oxidation of Se to SeCl4, which occurs at higher potentials, reduces the long-range order of crystalline Se and enables its discharge to Al2Se3. Solid-state 77Se nuclear magnetic resonance (NMR) measurements further establish that the local Se helical structures are maintained upon the loss of crystallinity.

Insights on the G protein-coupled receptor helix 8 solution structure and orientation using a neurotensin receptor 1 peptide.

G-protein coupled receptors (GPCRs) are the largest class of membrane proteins encoded in the human genome with high pharmaceutical relevance and implications to human health. These receptors share a prevalent architecture of seven transmembrane helices followed by an intracellular, amphipathic helix 8 (H8) and a disordered C-terminal tail (Ctail). Technological advancements have led to over 1000 receptor structures in the last two decades, yet frequently H8 and the Ctail are conformationally heterogeneous or altogether absent. Here we synthesize a peptide comprising the neurotensin receptor 1 (NTS1) H8 and Ctail (H8-Ctail) to investigate its structural stability, conformational dynamics, and orientation in the presence of detergent and phospholipid micelles, which mimic the membrane. Circular dichroism (CD) and nuclear magnetic resonance (NMR) measurements confirm that zwitterionic 1,2-diheptanoyl-sn-glycero-3-phosphocholine is a potent stabilizer of H8 structure, whereas the commonly-used branched detergent lauryl maltose neopentyl glycol (LMNG) is unable to completely stabilize the helix - even at amounts four orders of magnitude greater than its critical micellar concentration. We then used NMR spectroscopy to assign the backbone chemical shifts. A series of temperature and lipid titrations were used to define the H8 boundaries as F376-R392 from chemical shift perturbations, changes in resonance intensity, and chemical-shift-derived phi/psi angles. Finally, the H8 azimuthal and tilt angles, defining the helix orientation relative of the membrane normal were measured using paramagnetic relaxation enhancement NMR. Taken together, our studies reveal the H8-Ctail region is sensitive to membrane physicochemical properties and is capable of more adaptive behavior than previously suggested by static structural techniques.

Generalizing the Enhanced-Deep-Super-Resolution Neural Network to Brain MR Images: A Retrospective Study on the Cam-CAN Dataset.

The Enhanced-Deep-Super-Resolution (EDSR) model is a state-of-the-art convolutional neural network suitable for improving image spatial resolution. It was previously trained with general-purpose pictures and then, in this work, tested on biomedical magnetic resonance (MR) images, comparing the network outcomes with traditional up-sampling techniques. We explored possible changes in the model response when different MR sequences were analyzed. T1w and T2w MR brain images of 70 human healthy subjects (F:M, 40:30) from the Cambridge Centre for Ageing and Neuroscience (Cam-CAN) repository were down-sampled and then up-sampled using EDSR model and BiCubic (BC) interpolation. Several reference metrics were used to quantitatively assess the performance of up-sampling operations (RMSE, pSNR, SSIM, and HFEN). Two-dimensional and three-dimensional reconstructions were evaluated. Different brain tissues were analyzed individually. The EDSR model was superior to BC interpolation on the selected metrics, both for two- and three- dimensional reconstructions. The reference metrics showed higher quality of EDSR over BC reconstructions for all the analyzed images, with a significant difference of all the criteria in T1w images and of the perception-based SSIM and HFEN in T2w images. The analysis per tissue highlights differences in EDSR performance related to the gray-level values, showing a relative lack of outperformance in reconstructing hyperintense areas. The EDSR model, trained on general-purpose images, better reconstructs MR T1w and T2w images than BC, without any retraining or fine-tuning. These results highlight the excellent generalization ability of the network and lead to possible applications on other MR measurements.

Predicting the compressive strength of tight sandstone based on the low field NMR and pseudo-triaxial compression measurements

The compressive strength is very important for petroleum and other engineering studies. However, the effect of pore size and fluid distribution on the rock’s strength is not fully understood. We developed comprehensive research to study the controlling factors of the compressive strength based on low field nuclear magnetic resonance (NMR) measurements and pseudo-triaxial compression test for tight sandstones. The relationship between the compressive strength and the NMR obtained parameters are investigated completely, aiming for a better estimation of the compressive strength using the NMR data. The result shows that the rock’s strength is strongly controlled by the pore size distribution and the fluid existing state. Generally, the compressive strength is negatively correlated with the average transversal relaxation time, the movable water saturation, and the porosity, but positively correlated with the irreducible water saturation. The result reveals that the rock with larger pore radius and higher percentage of movable fluid is easier to reach the failure state. Further, the precision of the empirical model by multiple regression of the geometric mean of the relaxation time and the porosity is greatly improved compared with the model established by the brittle minerals, which is potentially to be use for geophysical prospecting when the NMR logging data is available.

Sensing spin wave excitations by spin defects in few-layer-thick hexagonal boron nitride.

Optically active spin defects in wide bandgap semiconductors serve as a local sensor of multiple degrees of freedom in a variety of "hard" and "soft" condensed matter systems. Taking advantage of the recent progress on quantum sensing using van der Waals (vdW) quantum materials, here we report direct measurements of spin waves excited in magnetic insulator Y3Fe5O12 (YIG) by boron vacancy [Formula: see text] spin defects contained in few-layer-thick hexagonal boron nitride nanoflakes. We show that the ferromagnetic resonance and parametric spin excitations can be effectively detected by [Formula: see text] spin defects under various experimental conditions through optically detected magnetic resonance measurements. The off-resonant dipole interaction between YIG magnons and [Formula: see text] spin defects is mediated by multi-magnon scattering processes, which may find relevant applications in a range of emerging quantum sensing, computing, and metrology technologies. Our results also highlight the opportunities offered by quantum spin defects in layered two-dimensional vdW materials for investigating local spin dynamic behaviors in magnetic solid-state matters.

Rate of Change in Cardiac Magnetic Resonance Imaging Measures Is Associated With Death in Duchenne Muscular Dystrophy.

Cardiovascular disease is the leading cause of death among patients with Duchenne muscular dystrophy (DMD). Identifying patients at risk of early death could allow for increased monitoring and more intensive therapy. Measures that associate with death could serve as surrogate outcomes in clinical trials. Duchenne muscular dystrophy subjects prospectively enrolled in observational studies were included. Models using generalized least squares were used to assess the difference of cardiac magnetic resonance measurements between deceased and alive subjects. A total of 63 participants underwent multiple cardiac magnetic resonance imaging and were included in the analyses. Twelve subjects (19.1%) died over a median follow-up of 5 years (interquartile range, 3.1-7.0). Rate of decline in left ventricular ejection fraction was faster in deceased than alive subjects (P<0.0001). Rate of increase in indexed left ventricular end-diastolic (P=0.0132) and systolic (P<0.0001) volumes were higher in deceased subjects. Faster worsening in midcircumferential strain was seen in deceased subjects (P=0.049) while no difference in global circumferential strain was seen. The rate of increase in late gadolinium enhancement, base T1, and mid T1 did not differ between groups. Duchenne muscular dystrophy death is associated with the rate of change in left ventricular ejection fraction, midcircumferential strain, and ventricular volumes. Aggressive medical therapy to decrease the rate of progression may improve the mortality rate in this population. A decrease in the rate of progression may serve as a valid surrogate outcome for therapeutic trials.

Disaggregation and fibrillation during sol-gel transition of alginate hydrogels

The rheological and morphological characteristics of Ca-crosslinked alginate hydrogels with two different M/G ratios, α-L-guluronate (G)-rich and β-D-mannuronate (M)-rich, each with one alginic acid concentration, were investigated. It was found that the stiffness and elasticity of alginate hydrogels are derived from the thickness and density of the fibril network structures. In aqueous alginate solution, ball-like aggregates of alginates are present. Time-resolved small-angle X-ray scattering and time-domain nuclear magnetic resonance measurements suggest that the disaggregation of alginate aggregates and loose fibrillation occur in the early stage of the sol-gel transition. After these induction stage, direct gelation is finally caused by the formation of the egg-box junction. G-rich alginate hydrogel has a higher stiffness and a thicker and denser fibril network structure than M-rich alginate hydrogel. The former also exhibits faster and more significant changes in physical properties during the sol-gel transition.