Magnetic Properties Of Nanoparticles Research Articles

Investigation of magnetic resonance contrast properties of PEG-coated gadolinium oxide nanoparticles in various biological environments

Abstract Magnetic nanoparticles (MNPs) are promising tools for biomedical applications, particularly in molecular imaging using magnetic resonance imaging (MRI). The unique magnetic properties of MNPs, combined with their similarity in size to biological objects, make them ideal candidates for in situ imaging probes. The present study explores the use of magnetic nanoparticles (MNPs) as contrast agents in magnetic resonance imaging (MRI) for improved diagnostic accuracy. Specifically, the study investigates the MR contrast properties of polyethylene glycol-coated gadolinium oxide nanoparticles (PEG@GONPs) in five different biological fluids. The nanoparticles were synthesized using the polyol route and their size, shape, and morphology were characterized using TEM, SEM, and FT-IR spectroscopy. The magnetic resonance (MR) relaxivity of PEG@GONPs was studied in different biologically relevant media, and results revealed highest relaxivity in plasma as compared to other media. In addition, comparative analysis of proton relaxivity of the synthesized nanoparticles was carried out with a well-known gadolinium-based contrast agent, Omniscan, in various medium. The present findings revealed that PEG@GONPs can serve as an effective contrast agent for MRI imaging in biological fluids such as plasma, which is crucial for preclinical diagnosis of specific diseases and lesions. The high relaxivity observed in plasma could be attributed to the interaction of the nanoparticles with plasma proteins, amplifying their magnetic properties which further improve their ability to produce contrast in MR images.

Structure and Magnetic Properties of Iron Oxide Nanoparticles Subjected to Mechanical Treatment

Iron oxide nanoparticles have been fabricated using the electric wire explosion (EWE) technique. The structure and magnetic properties of the nanoparticles have been analyzed before and after mechanical grinding in a ball mill for different time periods, focusing on potential bioapplications. The phase composition of the nanoparticles (70% Fe3O4, 30% Fe2O3) has remained unchanged despite the mechanical effects. The average nanoparticle size has not been affected either. The observation of the Verwey transition in the studied nanoparticles, along with the structural data, provides a better understanding of the physical properties of EWE ensembles of nanoparticles in different states. The analysis of the structure and magnetic properties reveals the development of a material with a high level of internal stress. This finding may be of interest for bioapplications due to its potential impact on the material performance.

Ranking Magnetic Colloid Performance for Magnetic Particle Imaging and Magnetic Particle Hyperthermia

AbstractMagnetic particle imaging (MPI) is an emerging modality that can address longstanding technological challenges encountered with magnetic particle hyperthermia (MPH) cancer therapy. MPI is a tracer technology compatible with MPH for which magnetic nanoparticles (MNPs) provide signal for MPI and heat for MPH. Identifying whether a specific MNP formulation is suitable for both modalities is essential for clinical implementation. Current models predict that functional requirements of each modality impose conflicting demands on nanoparticle magnetic properties. This objective here is to develop a measurement and ranking scheme based on end‐use performance to streamline evaluation of candidate MNP formulations. The measured MPI point‐spread function (PSF) and specific loss power (SLP) is combined to generate a single numerical value for comparison on a relative ranking scale, or figure of merit (FoM). 12 aqueous iron‐containing formulations are evaluated, including FDA‐approved (parenteral) iron‐containing colloids. MNPs with high (Synomag‐D70: 123.4), medium (Synomag‐D50: 63.2), and low (NanoXact: 0.147) FoM values are selected for in vivo validation of the selection scheme in subcutaneous 4T1 tumors. Results demonstrate that the proposed ranking accurately assessed the relative performance of MNPs for MPI and MPH. Data demonstrated that image quality and tumor temperature rise increased with FoM ranking, validating predictions. It isshown that the MPI signal correlated with MNP concentration in tissue. Computational heat transfer models anchored on tumor MPI data harmonized with experimental results to within an average of 2 °C when MNP content estimated from MPI data is included. Computational studies emphasized the importance of post‐injection MNP quantitation and MPI spatial resolution.

Microgravity influenced unsteady dispersion during magnetic drug targeting in an inclined tumor-stenosed microvessel with slip effects

Introduction:Recent cancer research into microgravity or altered gravity environments has gained attention for its potential to disrupt cancer cell growth and proliferation while reducing the risk of chemoresistance—a major challenge in conventional chemotherapy. Aim of the study:Motivated by the emerging applications of microgravity in cancer research, the current study aimed to mathematically investigate the potential of the microgravity-enhanced environment in combination with magnetic drug targeting (MDT) to optimize drug delivery and dispersion. Methodology:The particle velocity, including microgravity’s influence in the presence of an external applied magnetic field alongside fluid pressure, was computed analytically while the solute dispersion model was numerically evaluated using the Crank–Nicolson method. Results:Findings show that microgravity lowers particle concentration in the tumor, however, improved drug-loaded magnetic nanoparticle properties, and magnetic field strength reveal a positive impact on nanoparticle accumulation. Also, altered stenosis height, inclination angle, slip velocity, and other control parameters such as Peclet number, magnetic-tumor distance, drug source term, elimination, and local skin friction revealed significant differences in particle dispersion behavior before, within, and after the stenosis region. Conclusion:Our results suggest potential future therapeutic applications of altered gravity in combination with MDT phenomena to improve drug delivery for noninvasive therapeutic strategies.

β-Cyclodextrin and APTES functionalized magnetic iron oxide nanoparticles for improved lipase immobilization, transesterification efficiency, and reusability

This study focuses on the immobilization of Rhizopus oryzae lipase (ROL) on magnetic iron oxide nanoparticles (MNPs) through physical adsorption, taking advantage of the magnetic properties of the nanoparticles for easy separation. Different types of nano-biocatalysts were prepared by immobilizing the lipase enzyme on bare iron nanoparticles (Fe3O4), APTES-functionalized iron nanoparticles (Fe3O4-APTES), and β-cyclodextrin-functionalized iron nanoparticles (Fe3O4-APTES-GA-β_CD). These nano-biocatalysts were employed in the transesterification process. The synthesis of Fe3O4 nanoparticles was achieved using the coprecipitation method, and the structural, magnetic, and morphological properties of the iron nanoparticles (MNPs) were characterized through FTIR, XRD, VSM, SEM, and TEM analyses. The presence of strong physical bonds, resulting from hydrophilic and hydrophobic adsorption between β-cyclodextrin and the enzyme, led to an increased loading amount from 27.37% to 44.89%. The nano-biocatalysts exhibited enhanced thermal and storage stability compared to free lipase. In transesterification reactions, ROL/Fe3O4-APTES-GA-β_CD exhibited the highest conversion percentage of 64.3% among the functionalized enzymes. Furthermore, the reusability study demonstrated that ROL/Fe3O4-APTES-GA-β_CD retained 40.9% of the initial conversion percentage after five cycles, displaying the highest reusability among all nano-biocatalysts in this study.

Effect of precipitating agent, N2 gas, extract volume and pH on the magnetic properties of magnetite nanoparticles by green synthesis from aqueous pomegranate peel extract.

Superparamagnetic nanoparticles (SPMNPs) have attracted considerable attention in biomedicine, particularly magnetic hyperthermia for cancer treatment. However, the development of efficient and eco-friendly methods for synthesizing SPMNPs remains a challenge. This study reports on a green synthesis approach for SPMNPs using pomegranate peel extract as a stabilizing agent. The effects of various synthesis parameters, including the type of precipitating agent (NH3 and NaOH), N2 gas, extract volume, and pH, were systematically investigated with regard to the size, morphology, and magnetic properties of the nanoparticles. The results showed that reducing the volume of the extract increased the saturation magnetization of the nanoparticles. N2 gas was found to be essential in preventing the oxidation of the nanoparticles. The type of precipitating agent also affected the size and magnetization of the nanoparticles, with NaOH leading to the synthesis of SPMNPs with higher magnetization (∼4times) compared to NH3. Additionally, nanoparticles synthesized at pH 10 exhibited higher magnetization than those synthesized at pH 8 and 12. In conclusion, the optimized synthesis conditions significantly affected the magnetization and stability of SPMNPs. These nanoparticles are suitable for use in magnetic nanofluid hyperthermia applications.

Synthesis-Dependent Structural and Magnetic Properties of Monodomain Cobalt Ferrite Nanoparticles

This research examines the structural and magnetic properties of monodomain cobalt ferrite nanoparticles with the formula (Co1−xFex)A[Fe2−xCox]BO4. The particles were synthesized using various methods, including coprecipitation (with and without ultrasonic assistance), coprecipitation followed by mechanochemical treatment, microemulsion, and microwave-assisted hydrothermal techniques. The resulting materials were extensively analyzed using X-ray diffraction (XRD) and magnetic measurements to investigate how different synthesis methods affect the structure and cation distribution in nanoscale CoFe2O4. For particles ranging from 15.8 to 19.0 nm in size, the coercivity showed a near-linear increase from 302 Oe to 1195 Oe as particle size increased. Saturation magnetization values fell between 62.6 emu g−1 and 74.3 emu g−1, primarily influenced by the inversion coefficient x (0.58–0.85). XRD analysis revealed that as the larger Co2+ cations migrate from B- to A-sites (decreasing x), the lattice constants and inter-cation hopping distances increase, while the average strength of super-exchange interactions decreases. This study establishes a connection between the magnetic properties of the synthesized samples and their structural features. Importantly, this research demonstrates that careful selection of the synthesis method can be used to control the magnetic properties of these nanoparticles.

The Impact of Surface Modifier on Magnetic Nanoparticle Properties and Their Application in CD3+T Cell Separation.

Fe3O4 nanoparticles occupy a pivotal position in the realm of nanobiology due to their nontoxic, biocompatible, and superparamagnetic properties. This study examines the influence of surface modifiers on the properties of magnetic nanoparticles. Poly(methacrylic acid) (PMAA), poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (PSSM), trisodium citrate (TSC), carboxymethylcellulose (CMC), and carboxymethylated-dextran 40 (CMD40) were introduced into a one-pot solvothermal method to synthesize magnetic nanoparticles. TEM, the 4-(bromomethyl)-6,7-dimethoxy coumarin (BMMC) absorption assay, and the Bradford method were employed to characterize the diameter, carboxyl content, and protein immobilization ability of the nanoparticles, respectively. The findings revealed that CMD40-modified magnetic nanoparticles (CMD40-MNPs) exhibited the highest carboxyl content and streptavidin (SA) immobilization content, reaching 6.5 × 10-7 mol/mg and 375 μg/mg, respectively. In contrast, CMC-modified magnetic nanoparticles displayed opposite trends. This is primarily attributed to dextran's unique molecular structure, which enhances its water solubility and biocompatibility, thereby facilitating contact with Fe3O4 nanoparticles in aqueous solutions. CMD40-MNPs possess a saturation magnetization value of 60.90 emu/g and can be collected within (60 ± 5) s using a standard magnetic separator. Cytotoxicity assays demonstrated that CMD40-MNPs are nontoxic to cells. A cell sorting strategy utilizing the binding of SA-CMD40-MNPs and biotin antihuman CD3 antibody-modified cell suspensions was employed to isolate CD3+T cells. The results indicate that the purity and efficiency of targeted CD3+T cells are 85.2% and 61.5%, respectively.

Impact of Particle Size on the Nonlinear Magnetic Response of Iron Oxide Nanoparticles during Frequency Mixing Magnetic Detection.

Magnetic nanoparticles (MNPs), particularly iron oxide nanoparticles (IONPs), play a pivotal role in biomedical applications ranging from magnetic resonance imaging (MRI) enhancement and cancer hyperthermia treatments to biosensing. This study focuses on the synthesis, characterization, and application of IONPs with two different size distributions for frequency mixing magnetic detection (FMMD), a technique that leverages the nonlinear magnetization properties of MNPs for sensitive biosensing. IONPs are synthesized through thermal decomposition and subsequent growth steps. Our findings highlight the critical influence of IONP size on the FMMD signal, demonstrating that larger particles contribute dominantly to the FMMD signal. This research advances our understanding of IONP behavior, underscoring the importance of size in their application in advanced diagnostic tools.

A Short Appraisal of Magnetic Nanoparticles for Breast Cancer: In vitro and In vivo Research.

The increasing incidence of breast cancer and the associated morbidity due to higher metastasis created the urge to develop a nanocarrier that can be used as a potent therapeutic carrier with targeting efficacy. The use of superparamagnetic nanoparticles in breast cancer research and treatment has gained considerable attention in recent years. Magnetic nanoparticles (MNPs) can be used to construct nanocarriers since they possess superior properties such as superparamagnetism, easy surface functionalization to attach ligands, and non-immunogenic. MNPs are superior carriers that are used to target cancer cells without harming the normal cells in the body, which leads to therapeutic efficacy in the body. Along with their established anticancer potential and enhanced drug concentration at endosomal pH, the superparamagnetic property of MNPs was further exploited for their applications in reticuloendothelial uptake, drug delivery, medical imaging, and theranostics applications in breast cancer. Moreover, the clinical translational of MNPs, along with future prospects and key challenges in vivo, have been duly presented in the final review. The scientists preferred the ongoing research in MNPs due to their high biocompatibility and ease of targeting at molecular and cellular levels. The review highlighted the in vitro and in vivo research and patent supported data for potential use of MNPs for the treatment of breast cancer.

Concentration dependent thermal properties of magnetic nanoparticles and ability to use in the magnetic hyperthermia treatment

Concentration dependent thermal properties of magnetic nanoparticles and ability to use in the magnetic hyperthermia treatment

Surface-engineered Multimodal Magnetic Nanoparticles: Scope and Applications

Abstract: Magnetic nanoparticles (MNPs) are one of the classes of nanoparticles that produce magnetic properties by manipulation using a magnetic field. These MNPs are currently used in the theranostics disease modification of surface properties of MNPs, achieved by surface engineering, which provides different applications, such as enhancing thermal and optical properties, biocompatibility, conductivity and also mechanical and chemical properties. Advancement in the technology leads to the development of MNPs. For this instance, surface coating is used at molecular and cellular levels. The uses of coating material are based on organic and inorganic molecules, including polymer, liposomes, micelles, metal oxide, gold-NPs etc. Using different coatings exhibits multifunction properties, and this helps reduce toxicity, helps in biomedical applications, maintains stability, restricts surface fouling, etc. This review provides an in-depth knowledge of surface-engineered magnetic nanoparticles (MNPs) according to recent developments.

Key Contributors to Signal Generation in Frequency Mixing Magnetic Detection (FMMD): An In Silico Study.

Frequency mixing magnetic detection (FMMD) is a sensitive and selective technique to detect magnetic nanoparticles (MNPs) serving as probes for binding biological targets. Its principle relies on the nonlinear magnetic relaxation dynamics of a particle ensemble interacting with a dual frequency external magnetic field. In order to increase its sensitivity, lower its limit of detection and overall improve its applicability in biosensing, matching combinations of external field parameters and internal particle properties are being sought to advance FMMD. In this study, we systematically probe the aforementioned interaction with coupled Néel-Brownian dynamic relaxation simulations to examine how key MNP properties as well as applied field parameters affect the frequency mixing signal generation. It is found that the core size of MNPs dominates their nonlinear magnetic response, with the strongest contributions from the largest particles. The drive field amplitude dominates the shape of the field-dependent response, whereas effective anisotropy and hydrodynamic size of the particles only weakly influence the signal generation in FMMD. For tailoring the MNP properties and parameters of the setup towards optimal FMMD signal generation, our findings suggest choosing large particles of core sizes dC>25 nm with narrow size distributions (σ<0.1) to minimize the required drive field amplitude. This allows potential improvements of FMMD as a stand-alone application, as well as advances in magnetic particle imaging, hyperthermia and magnetic immunoassays.

Magnetic Nanoparticles: Synthesis, Characterization, and Their Use in Biomedical Field

In recent years, the use of magnetic nanoparticles (MNPs) in biomedical applications has gained more and more attention. Their unusual properties make them ideal candidates for the advancement of diagnosis, therapy, and imaging applications. This review addresses the use of MNPs in the field of biomedicine encompassing their synthesis, biofunctionalization, and unique physicochemical properties that make them ideal candidates for such applications. The synthesis of magnetic nanoparticles involves a range of techniques that allow for control over particle size, shape, and surface modifications. The most commonly used synthesis techniques that play a crucial role in tailoring the magnetic properties of nanoparticles are summarized in this review. Nevertheless, the main characterization techniques that can be employed after a successful synthesis procedure are also included together with a short description of their biomedical applications. As the field of magnetic nanoparticles in biomedical applications is rapidly evolving, this review aims to serve as a valuable resource, especially for young researchers and medical professionals, offering basic but very useful insights into recent advancements and future prospects in this highly interdisciplinary research topic.

Size effect on the structural and magnetic phase transformations of iron nanoparticles.

Iron nanoparticles are among the most promising low-dimensional materials in terms of applications. This particularity is attributable to the magnetic properties of these nanoparticles, which exhibit different allotropes as a function of temperature. In this work, we sought to characterise at the atomic scale how their structural and magnetic transformations can be affected by the size. To achieve this objective, we developed a tight-binding model incorporating a magnetic contribution via a Stoner term implemented in a Monte Carlo code to relax the structure and the magnetic state. Using our approach, we show that magnetism is strongly reinforced by the surface, which leads to an increase in the Curie temperature as the size of the particle decreases contrary to the solid-solid transition temperature. Our work thus provides a deep understanding at the atomic scale of the key factors that determine the structural and magnetic properties of Fe nanoparticles, shedding more light on their unique character, which is crucial for further applications.

Magnetic nanoparticles and possible synergies with cold atmospheric plasma for cancer treatment.

The biomedical applications of magnetic nanoparticles (MNPs) have gained increasing attention due to their unique biological, chemical, and magnetic properties such as biocompatibility, chemical stability, and high magnetic susceptibility. However, several critical issues still remain that have significantly halted the clinical translation of these nanomaterials such as the relatively low therapeutic efficacy, hyperthermia resistance, and biosafety concerns. To identify innovative approaches possibly creating synergies with MNPs to resolve or mitigate these problems, we delineated the anti-cancer properties of MNPs and their existing onco-therapeutic portfolios, based on which we proposed cold atmospheric plasma (CAP) to be a possible synergizer of MNPs by enhancing free radical generation, reducing hyperthermia resistance, preventing MNP aggregation, and functioning as an innovative magnetic and light source for magnetothermal- and photo-therapies. Our insights on the possible facilitating role of CAP in translating MNPs for biomedical use may inspire fresh research directions that, once actualized, gain mutual benefits from both.

Interplay of Anisotropy Energy Barrier and Self-Heating Efficiency of Cobalt-Substituted CuFe2O4 Nanoparticles.

In this study, we delve into the intricate interplay between the anisotropy energy barrier and the self-heating efficiency of magnetic nanoparticles (MNPs). We embarked on this exploration by synthesizing Cu1-xCoxFe2O4 (x = 0, 0.1, 0.3, and 0.5) MNPs using a straightforward coprecipitation method. Our magnetic assessments, conducted at different temperatures, unveiled a notable trend as we traversed from x = 0.1 to x = 0.5. Specifically, we observed a consistent increase in saturation magnetization, coercivity, and remanence. This pattern also extended to the anisotropy energy barrier, which was derived from the effective anisotropy constant determined through the temperature dependency of the coercivity method. However, an intriguing twist emerged when we scrutinized the specific absorption rate (SAR), calculated via the Box-Lucas method. Contrary to much of the existing literature, our experimental results showcased a decline in SAR concerning x. This experimental work challenges the conventional understanding of the relationship between the anisotropy energy barrier and the SAR value of these nanoparticles. This study prompts us to reconsider the intricate mechanisms governing the relaxation of magnetic moments and subsequent heat release when subjected to an alternating magnetic field. By doing so, we aim to gain fresh insights into the self-heating properties of MNPs and optimize their utilization to better understand their heat-release properties and ensure that they are used as efficiently as possible in a variety of biomedical applications.

Oxygen vacancies mediated changes in ferromagnetic, optical, Photoluminescent and electronic structure properties of Ho-doped CeO2 nanoparticles

The undoped CeO2 and Ce1-xHoxO2 (x = 0.03, 0.05 and 0.07) nanoparticles prepared using microwave assisted co-precipitation route are investigated using X-Ray Diffraction (XRD), UV–Vis–NIR, Raman, photoluminescence, X-ray Photoelectron Spectroscopy (XPS) and SQUID-VSM magnetometer. The XRD validates the inclusion of Ho3+ at the Ce3+/Ce4+ site while maintaining the crystal structure with development of defects and oxygen vacancies with expansion of the CeO2 nanolattice. The composition of the elements with desired stoichiometry is confirmed by Energy Dispersive X-ray spectra analysis. The absorption spectroscopy analysis reports the Urbach energy along with refractive index increases with Ho3+ doping and the optical band gap is decreased indicating red shift. All the nanoparticles are subjected to PL investigations to ascertain the oxygen vacancy and defect structure of nanoparticles. Raman active modes at ∼500 - 630 cm−1 verifies the existence of oxygen vacancies (Vo)., which are seen to rise with concentration of Ho-doping in the CeO2 lattice. When Ce4+ transforms into Ce3+, oxygen vacancies are generated in the lattice to maintain the charge neutrality, as can be seen by the Ce-3d, O-1s, and Ho-4d core level XPS spectra. To demonstrate that Ho3+ doping favours ferromagnetic ordering in the CeO2 lattice, the magnetic properties using the Bound Magnetic Polaron model and F centre exchange mechanism are discussed. The development of various complexes that involve host and doped cations are mediated by Vo suggests the existence of F0, F+, and F2+ centres. The weak ferromagnetic behaviour in Ho3+-doped CeO2 nanoparticles reinforce the relationship between Vo to explain the observed magnetic properties of nanoparticles.

Structural, Magnetic, and Mössbauer Investigation of Mg-Ni-Co ferrites doped by Sm3+ sions

Samarium-doped magnesium-nickel-cobalt nanoferrites (Mg1/3 Ni1/3Co1/3)Fe2−xSmxO4, with x = 0.00, 0.01, 0.02, 0.04, and 0.08, were synthesized by the coprecipitation method. X-ray diffractometer (XRD), transmission electron microscopy (TEM), energy dispersive x-ray (EDX), x-ray photoelectric spectroscopy (XPS), Fourier Transform Infra-Red (FTIR), Raman spectroscopy, Mössbauer spectroscopy, and magnetic measurement techniques were used, to study the structure, microstructure, and magnetic properties of the samples. The formation of the cubic spinel structure was confirmed by Rietveld analysis of the XRD data and by the appearance of the two absorption bands close to 400 cm−1 and 600 cm−1 from the FTIR spectrum. Raman spectroscopy verified the formation of the spinel phase in the samples. The elemental composition, valency, and cationic distribution were examined using x-ray photoelectric spectroscopy (XPS) measurements. Experimental findings revealed that doping with Sm3+ ions had a significant effect on the magnetic properties of nanoparticles. The saturation magnetization (M s ) and coercivity field (H c ) values fluctuate depending on the crystallite size (DXRD) of the samples from XRD analysis as the Sm3+ content increases. The magnetization dependence on the applied field was investigated at different ranges of applied fields based on the output of the statistical parameters for the curve fitted using four different forms of the law of approach to saturation. The statistical parameters and physically significant fitted parameters give information on the dependence of magnetization over various applied field regions. A thorough investigation of the output parameters from fitting into various equations reveals that the composition of Mg-Ni-Co ferrites exhibits a dependence of magnetization on the applied field. Room-temperature Mössbauer spectra displayed a mix of the magnetic sextet and central quadrupole doublet, with improvement in the magnetic sextet in the Sm-doped samples. Moreover, Mössbauer spectra at 77 K showed the demise of the quadrupole doublet in all samples and showed two sextets (tetrahedral and octahedral sites). Sm-doping reduced the values of the hyperfine magnetic field of both sextets. All Fe ions can be found in the Fe3+ state, according to the isomer shift values and there is a migration of Fe3+ ions from octahedral to tetrahedral sites upon Sm doping, which was confirmed by XPS measurements.

Influence of hydrogel and porous scaffold on the magnetic thermal property and anticancer effect of Fe3O4 nanoparticles

Magnetic hyperthermia uses magnetic nanoparticles (MNPs) for conversion of magnetic energy into thermal energy under an alternating magnetic field (AMF) to increase local temperature for ablation of cancer cells. The magnetic thermal capacity of MNPs not only depends on the intrinsic properties of MNPs but is also affected by the microenvironmental matrices surrounding the MNPs. In this study, the influence of agarose hydrogels and gelatin porous scaffolds on the magnetic thermal property and anticancer effect of Fe3O4 nanoparticles (NPs) were investigated with a comparison to free Fe3O4 NPs. Flower-like Fe3O4 NPs were synthesized and embedded in agarose hydrogels and gelatin porous scaffolds. Under AMF irradiation, the free Fe3O4 NPs had the best magnetic thermal properties and the most efficiently increased the local temperature to ablate breast cancer cells. However, the Fe3O4 NPs embedded in agarose hydrogels and gelatin porous scaffolds showed reduced magnetic-thermal conversion capacity, and the local temperature change was decreased in comparison to free Fe3O4 NPs during AMF irradiation. The gelatin porous scaffolds showed a higher inhibitory influence than the agarose hydrogels. The inhibitory effect of agarose hydrogels and gelatin porous scaffolds on magnetic-thermal conversion capacity resulted in a decreased anticancer ablation capacity to breast cancer cells during AMF irradiation. The Fe3O4 NP-embedded gelatin scaffolds showed the lowest anticancer effect. The results suggested that the matrices used to deliver MNPs could affect their performance, and appropriate matrices should be designed to maximize their therapeutic effect for biomedical applications.