Magnetic Mesoporous Silica Microspheres Research Articles

Interfacial Si–O–Zr bonds induced well dispersed zirconia on magnetic mesoporous silicas for enhanced tetracycline removal

Constructing recoverable materials with both high adsorption capacity and excellent photocatalytic performance towards antibiotics elimination is very meaningful and challenging in wastewater remediation. In this work, we demonstrate a simple interface engineering strategy to design well dispersed zirconia on core–shell structured magnetic mesoporous silica microspheres (MMS) for efficient removal of tetracycline hydrochloride (TC). With ultrahigh specific surface area (682.0 m2/g) and interfacial Si-O-Zr serving as active sites, the optimal sample (MMS-Zr10) exhibits enhanced TC adsorption capacity of 266.5 mg/ZrO2 g, which is 19.2-fold than that of pure ZrO2. Simultaneously, the photocatalytic degradation rate constant of TC over MMS-Zr10 in the presence of peroxydisulfate (PDS) is 12.5 times that of ZrO2 under visible light irradiation, which may be attributed to the enhanced light harvest capability and high charge separation efficiency facilitated by interfacial Zr–O–Si charge transfer channels. Furthermore, in consecutive adsorption cycles in both deionized (DI) water solution and actual wastewater, no obvious decline in TC removal % is observed. The TC adsorption is mainly governed by pore filling, hydrogen bonding and electrostatic interactions; whilst O2•−, h+, SO4•−, •OH and 1O2 species are responsible for enhanced photocatalytic degradation of TC.

Ion-imprinted magnetic adsorbents for the selective capture of Cu(II) and their cascade application as heterogeneous recyclable catalysts for Ullmann and Glaser coupling reactions

The selective capture and efficient reuse of Cu(II) from contaminated seawater requires a multifunctional adsorbent with a high Cu(II) selectivity and magnetic properties to facilitate recycling. Moreover, the cascade application of the recycled adsorbent is important but has not been extensively investigated. Accordingly, in this study, ion-imprinted magnetic adsorbents, particularly, copper ion-imprinted polymers (Fe3O4@mSiO2@CIIP) were prepared using magnetic mesoporous silica microspheres (Fe3O4@mSiO2) as carriers, with organosilicon containing hydroxyl and Schiff bases as the polymer backbone to selectively collect Cu(II) from aqueous media. The adsorption isotherms, kinetics, selectivity, and mechanisms were examined under various adsorption conditions. Fe3O4@mSiO2@CIIP-2 shows high selectivity and fast adsorption kinetics, and the equilibrium adsorption amount of Cu(II) is 263.2 mg/g within 30 min. The pseudo-second-order model and the Langmuir model are supported by the adsorption kinetics and isotherms, respectively. Particularly, the adsorption amount of Fe3O4@mSiO2@CIIP-2 could be reused eight times without substantial loss. Additionally, specific functional monomers bound to Fe3O4@mSiO2@CIIP-2 could both selectively adsorb Cu and serve as functional ligands for catalysts that can heterogeneously catalyze the Ullmann and Glaser coupling reactions after adsorbing Cu(II).

Iron tailings derived core-shell magnetic mesoporous silica microspheres for effective Congo red removal

Iron tailings derived core-shell magnetic mesoporous silica microspheres for effective Congo red removal

Adsorptive removal of Cr(III)-EDTA chelate from an aqueous solution by magnetic mesoporous silica microspheres.

The 3-aminopropyltrimethoxysilane-modified magnetic mesoporous adsorbent (FNMs/APTES) was synthesized and applied to remove Cr(III)-EDTA chelates from water. The characterization of FNMs/APTES showed that the prepared adsorbent with a magnetic mesoporous structure was successfully grafted by APTES, which has good stability under acid conditions. The maximum capacities of FNMs/APTES for Cr(III)-EDTA adsorption at 15, 25 and 35 °C and pH 4.0 were 12.58, 13.13 and 14.00 mg·g-1, respectively. The adsorption isotherm of FNMs/APTES for Cr(III)-EDTA conforms to the Freundlich model, and the adsorption kinetic model accords with the pseudo-second-order kinetic model. Adsorption of Cr(III)-EDTA on the adsorbent was not affected in the presence of Na+, K+ and Ca2+ even at 100 mmol·L-1. Cr(III)-EDTA was anchored on FNMs/APTES through electrostatic interaction between protonated amino groups of adsorbents and Cr(III)-EDTA anions, and Cr(III)-EDTA chelates were adsorbed as a whole on the adsorbent. The Cr(III)-EDTA-saturated adsorbent can be readily regenerated in HCl solution and 83.03% of the initial Cr(III)-EDTA adsorption capacity remains after four adsorption-regeneration experiment cycles. The results highlighted that the FNMs/APTES as a potential adsorbent can be applied for the minimization of Cr(III)-EDTA chelates from water.

Adsorption performance of magnetic mesoporous silica microsphere support toward the remediation of acetaminophen from aqueous solution

Magnetic mesoporous silica microspheres (MSMS) were prepared in the present study and the removal of acetaminophen from aqueous solution by the MSMS was evaluated. The prepared MSMS were characterized to understand the surface morphology and magnetization capacity of the adsorbent. The Brauner-Emmett-Teller and vibrating sample magnetometer analysis of the MSMS revealed a large surface area (SBET – 273.8 m2/g), average pore size (26 nm) and a saturation magnetization of 8.3 emu/g which was ideal for the adsorption of contaminants. On optimizing the operating parameters, the maximum removal of the acetaminophen was achieved at pH 5.0, adsorbent dose of 1 g/L and initial adsorbate concentration of 150 mg/L. Further, during the maximum removal, the equilibrium time for acetaminophen was 30 min. The Langmuir adsorption capacity of the MSMS was estimated to be 310.7 mg/g for acetaminophen. The tested models (Langmuir, Freundlich, and Temkin models) showed excellent fits to the adsorption equilibrium data. Further, from the desorption studies, it was observed that the MSMS could be reused for more than 14 adsorption-desorption cycles for the contaminant.

Determination of polymer additives in foods and drinks packed with plastic by amino group modified magnetic mesoporous silica microspheres coupled with high performance liquid chromatography

The polymer additives may migrate out from polymer plastics into foods and drinks and their potential risk should not be ignored. In this study, amino group modified magnetic mesoporous silica microspheres were prepared by an in situ method. The magnetic microspheres as adsorbent of magnetic solid-phase extraction (MSPE) were coupled with high performance liquid chromatography (HPLC) to determine nine kinds of polymer additives including antioxidants and ultraviolet absorbers. An excellent linearity (0.9999 ≥ R 2 ≥ 0.9994) was at the range of 1.25–1000 ng/mL under optimized conditions. The limits of detection (LODs) and quantification were 0.01–0.09 ng/mL and 0.02–0.30 ng/mL, respectively, for the nine polymer additives, which was lower than those previously reported. This method can be used for the quantitative determination of the migration levels of seven kinds of polymer additives in real foods and drinks packed with plastics.

Structure Engineering of Yolk-Shell Magnetic Mesoporous Silica Microspheres with Broccoli-Like Morphology for Efficient Catalysis and Enhanced Cellular Uptake.

Yolk-shell magnetic mesoporous microspheres exhibit potential applications in biomedicine, bioseparation, and catalysis. Most previous reports focus on establishing various interface assembly strategies to construct yolk-shell mesoporous structures, while little work has been done to control their surface topology and study their relevant applications. Herein, a unique kind of broccoli-like yolk-shell magnetic mesoporous silica (YS-BMM) microsphere is fabricated through a surfactant-free kinetic controlled interface assembly strategy. The obtained YS-BMM microspheres possess a well-defined structure consisting of a magnetic core, middle void, mesoporous silica shell with tunable surface roughness, large superparamagnetism (36.4 emu g-1 ), high specific surface area (174 m2 g-1 ), and large mesopores of 10.9nm. Thanks to these merits and properties, the YS-BMM microspheres are demonstrated to be an ideal support for immobilization of ultrafine Pt nanoparticles (≈3.7nm) and serve as superior nanocatalysts for hydrogenation of 4-nitrophenol with yield of over 90% and good magnetic recyclability. Furthermore, YS-BMM microspheres show excellent biocompatibility and can be easily phagocytosed by osteoclasts, revealing a potential candidate in sustained drug release in orthopedic disease therapy.

Facile Synthesis of Hydrophilic Magnetic Mesoporous Silica Microspheres for Selective Enrichment of Glycopeptides and Glycans

Hydrophilic magnetic mesoporous silica microspheres with core-shell structure were prepared via a facile approach and applied as an enrichment probe for low-abundance glycopeptides and glycans from complex biological samples. The prepared mesoporous silica microspheres modified with gluconamide groups at the interior surface of mesopore channels showed more prominent features with respect to high specific surface area (490 m2/g), narrow pore size distribution (2.5 nm), and high saturation magnetization (23.87 emu/g), endowing them with hydrophilicity and size-exclusion selectivity. Taking these advantages of the size-exclusion and the hydrophilic interactions, the hydrophilic mesoporous magnetic microspheres exhibited specificity for glycopeptides in a digest of horseradish peroxidase. The detection limit for horseradish peroxidase was 25 fmol. In addition, the hydrophilic mesoporous magnetic microspheres were further applied to the selective enrichment of N-linked glycans from a digest of human serum. The successful application demonstrates the potential of the hydrophilic mesoporous magnetic microspheres in glycoproteomic analysis.

Rapid determination of organophosphorus pesticides in edible vegetable oils by direct microextraction using magnetic mesoporous silica microspheres

AbstractThis study describes a rapid and sensitive method for the detection of four organophosphorus pesticides in edible vegetable oils by magnetic mesoporous silica microsphere (mSiO2@Fe3O4) extraction. Organophosphorus pesticides were directly extracted from oil sample using mSiO2@Fe3O4 after diluting with n‐hexane. Compared with traditional sample preparation methods, this procedure is less time consuming and it does not necessitate environmentally unfriendly solvent extraction / centrifugation processes. Moreover, the matrix effect can also be suppressed by size‐exclusion interaction due to mesoporous structure of mSiO2@Fe3O4 and large molecules of fat. Clean analytes were analyzed by liquid chromatography with tandem mass spectrometry. Variables affecting extraction of organophosphorus pesticides were optimized. Under the optimized conditions, the whole pretreatment process could be accomplished within 10 min. Acceptable linearity in the range of 5–1000 ng/g was obtained for four organophosphorus pesticides with satisfactory R2 of 0.9858∼0.9983. The limits of detection ranged from 0.2 to 0.6 ng/g. The method recoveries at three spiked concentration levels (10, 100 and 1000 ng/g) were from 92.1 to 108.6% with interday and intraday relative standard deviations less than 5.9 and 13.4%, respectively. Finally, the established method was successfully applied for the determination of organophosphorus pesticides in real edible vegetable oils.

Investigating the high gradient magnetic separator function for highly efficient adsorption of lead salt onto magnetic mesoporous silica microspheres and adsorbent recycling

Herein, a novel magnetite core decorated with amine functionalized mesoporous silica include significant surface area (ca. 885 m2 g−1) has been successfully prepared. Cetyltrimethylammonium bromide as a pore-forming agent was eliminated from the structure through a combination of two methods (calcination and modified extraction via supercritical CO2). This inventive strategy leads to form magnetic mesoporous silica microspheres that enhance the capacity of adsorption of Pb2+, (ca. 989 mg g−1). In addition, the recycling of this adsorbent from aqueous medium using high gradient magnetic separation (HGMS) was also explored and for obtaining the efficiency of separation some tests were accomplished using different matrix geometries. The separation efficiency of adsorbent via fine-matrix was more successful than a coarse matrix. Utilizing a combined arrangement of a fine and a coarse matrix, the performance of the separation was increased considerably comparing the single-stage separation chamber. About 93% of the magnetic adsorbent could be separated when the flow rate and magnitude of the magnetic field were fixed at 7.2 mL s−1 and 0.33 m T, respectively. Generally, this work was developed high efficient adsorbent for water treatment and well planned separation of adsorbent from treated water by HGMS that can be used in a large-scale.

Identification of ligands from natural products as inhibitors of glutathione S-transferases using enzyme immobilized mesoporous magnetic beads with high-performance liquid chromatography plus quadrupole time-of-flight mass spectrometry and molecular docking.

Multidrug resistance is recognized as one of the main reasons leading to the failure of chemotherapy. Studies have shown that glutathione S-transferase inhibitors could be regarded as multidrug resistance reversal agents. Herein, a method of applying enzyme immobilization, molecular docking, and high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was employed to screen glutathione S-transferase inhibitors from natural products. Magnetic mesoporous silica microspheres were synthesized and modified with a poly(dopamine) layer, which has a large quantity of amino, enabling further non-covalent binding with glutathione S-transferase. Moreover, the immobilization conditions, namely, potential of hydrogen, catalase concentration, reaction temperature and reaction time, were optimized. In total, six potential compounds were isolated and identified from Perilla frutescens (L.) Britt leaves and green tea and molecular docking was applied to identify the binding site. Rosmarinic acid, (-)-epigallocatechin-3-O-gallate and (-)-epicatechin-3-gallate showed higher binding affinity than the compounds, and their half maximal inhibitory concentration values were further determined. The results suggested that this proposed method was effective and convenient for identifying glutathione S-transferase inhibitors from natural products.

Screening and analysis of cyclooxygenase-2 inhibitors from the complex matrix: A case study to illustrate the important effect of immobilized enzyme activity in magnetic ligand fishing

Magnetic ligand fishing is one of the most widely used methods to screen and separate potentially bioactive compounds from complex mixtures. However, it is poorly understood the ambiguous relations between immobilized enzyme activity and fishing results (accuracy and sensitivity). Therefore, to investigate the underlying relationship of them, we fabricated the immobilized enzyme with different activities by introducing a novel support material, nickel ions (Ni2+) functionalized magnetic mesoporous silica microspheres (MMSM@PDA-Ni2+). It possesses a higher activity than the previous report because of site-directed immobilization. Then, the immobilized COX-2 with different activities were prepared using different immobilization methods, including Ni2+ affinity-oriented immobilization, polydopamine (PDA) covalent immobilization, and conventional glutaraldehyde (GA) covalent immobilization. A standard mixture (COX-2 inhibitors and noninhibitors) and green tea extract were used to compare and evaluate the fishing results systematically. It displayed the fished inhibitors were dramatically reduced with the decreased activity of immobilized COX-2, indicating that immobilized enzyme activity played a critical role in a reliable magnetic ligand fishing analysis. This development questions some of the previous studies aimed at rapid screening bioactive compounds in natural products and opens new possibilities for accurate and sensitive magnetic ligand fishing analysis. Also, the introduced novel materials with mild immobilization strategy preserve enzyme activity and spatial conformational, therefore, provides a valuable tool in future applications.

Fabrication of hierarchical BiOCl-CoP heterojunction on magnetic mesoporous silica microspheres with double-cavity structure for effective photocatalysis

In this research, the magnetic mesoporous silica microspheres with double-cavity structure (DHFS) were first synthesized by combining the hydrothermal technique, sol-gel hydrolysis and chemical etching method, then the BiOCl-CoP-x (BC-x, x = 1, 2, 3) heterojunctions were fabricated on the surface of the amino-functionalized DHFS microspheres. The morphologies, crystal characteristics, compositions and photoelectrochemical properties of the samples in different stages were characterized by various technologies. Furthermore, methylene blue (MB), as the targeted organic pollutant, was used to study the enriching property and activity of the photocatalysts by the corresponding adsorption and degradation experiments. Research showed that the introduction of the DHFS microspheres could significantly enhance the adsorption ability of the photocatalyst for the existence of concentration gradient effect of the mesoporous and double-cavity structure, so that the DHFSBC-2 possessed the most superior activity under visible-light illumination (VLI) for the effective fabrication of heterojunction interfaces between BiOCl and CoP NPs. More importantly, this photocatalyst exhibited very strong photo-stability, and no decrease of the photocatalytic activity could be observed after being recycled for several times.

Core–Shell Magnetic Mesoporous Silica Microspheres with Large Mesopores for Enzyme Immobilization in Biocatalysis

Magnetic mesoporous silica microspheres with core-shell structure and large pores are highly desired in macromolecules delivery and biocatalysis, biospeparation, and adsorption. In this work, a controllable solvent evaporation induced solution-phase interface co-assembly approach was developed to synthesize core-shell structural magnetic mesoporous silica microspheres with ultralarge mesopore size (denoted as LP-MMS). The synthesis was achieved by employing large-molecular-weight amphiphilic block copolymers poly(ethylene oxide)- block-poly(methyl methacrylate) (PEO- b-PMMA) and small surfactant cetyltrimethylammonium bromide as co-templates, which can co-assemble with silica source in tetrahydrofuran/water solutions. The obtained LP-MMS microspheres possess uniform rasberry-like morphology with a diameter of 600 nm, large primary spherical mesopores (ca. 36 nm), large specific surface area (348 m2/g), high specific pore volume (0.59 cm3/g), and fast magnetic responsivity with high magnetization (15.9 emu/g). The mesopore morphology can be transformed from spherical to cylindrical through introducing a shearing force during the interfacial co-assembly in the synthesis system. The designed LP-MMS microspheres turn out to be good carriers for enzyme (trypsin) immobilization with a high loading capacity of 80 μg/mg and demonstrate excellent biocatalysis efficiency up to 99.1% for protein digestion within 30 min and good recycling stability with negligible decay in digestion efficiency after reuse for five times.

Smart Hydrophilic Modification of Magnetic Mesoporous Silica with Zwitterionic l-Cysteine for Endogenous Glycopeptides Recognition

There are still few reports about endogenous glycosylated peptides although the significance of the study of them has been confirmed since they may provide higher sensitivity and specificity in clinical practice. In this work, magnetic mesoporous silica microspheres were endowed with strong hydrophilicity by a simple and smart modification way to l-cysteine, which was attributed to the bond formation of Fe–S. The microsphere (denoted as l-Cys-Fe3O4@mSiO2) was composed of Fe3O4 core, mesoporous silica layer, and innumerable perpendicularly aligned mesopores, as well as abundant l-cysteine in the mesopores. Thanks to the excellent hydrophilicity of l-cysteine probe, the l-Cys-Fe3O4@mSiO2 microspheres showed strong recognition ability toward glycopeptides. Owing to the superparamagnetic cores well encapsulated in microspheres, the whole process is quite time-saving by magnetic separation. Also, due to the appropriate pore size of the l-Cys-Fe3O4@mSiO2 microspheres, the extraction method exhibited good size-e...

Hydrophilic phytic acid-functionalized magnetic dendritic mesoporous silica nanospheres with immobilized Ti4+: A dual-purpose affinity material for highly efficient enrichment of glycopeptides/phosphopeptides

In this work, magnetic mesoporous silica microspheres (Mag-MSMs) with ordered radial mesochannels were fabricated by a self-assembly synthesis in chlorobenzene-water mixed system. Then, the obtained Mag-MSMs were modified with polyethyleneimine (PEI), phytic acid (PA) and Ti4+ (denoted as Mag-MSMs@PEI-PA-Ti4+) via layer by layer (LbL) assembly. Due to the excellent hydrophilicity of PEI and PA and the large amount of Ti4+, the Mag-MSMs@PEI-PA-Ti4+ possessed combined properties of hydrophilic interaction liquid chromatography (HILIC)- and immobilized metal ion affinity chromatography (IMAC)-based materials, which could be used as a dual-purpose material for N-glycopeptides or phosphopeptides enrichment. The proposed Mag-MSMs@PEI-PA-Ti4+ exhibited an outstanding performance for N-glycopeptides enrichment (selectivity: IgG/BSA = 1:1000; sensitivity: 0.5 fmol/μL IgG) and phosphopeptides enrichment (selectivity: α-casein/BSA=1:5000; sensitivity: 0.2 fmol/μL α-casein). Furthermore, after enrichment with Mag-MSMs@PEI-PA-Ti4+, a total of 276 N-glycopeptides assigned to 132 glycoproteins were identified from 2 μL human serum and 1645 phosphopeptides corresponding to 704 phosphoproteins were identified from 200 μg HeLa cell extracts.

Synthesis of recyclable magnetic mesoporous RH-FSBA photoelectrocatalyst with double cavity structure

In this paper, a series of BiOCl-Ag/AgBr heterojunctions supported by magnetic mesoporous silica microspheres with double cavity structure (RH-FSBAx) were successfully synthesized. For the combination of double cavity structure and orderly mesoporous opening structure of magnetic mesoporous RH-FSBAx photoelectrocatalysts, they exhibited ultra-strong enriching property to targeted pollutants. For the formation of BiOCl-AgBr heterojunctions, superior photosensitization of AgBr and existence of metallic Ag plasmas with surface plasmon resonance (SPR) effect, the photoelectrocatalysts exhibited enhanced visible light photoelectrocatalytic performance. Furthermore, the photoelectrocatalyst can be recycled easily by magnetic separation for its superior magnetic response. Particularly, the magnetic mesoporous RH-FSBA0.03 photoelectrocatalyst exhibited the highest photoelectrocatalytic activity (k = 1.3 × 10−2 min−1) in degradation of acid fuchsin (AF) under visible light irradiation, and it was about 4 times of RH-FSB photoelectrocatalyst. Therefore, this novel photoelectrocatalyst can effectively decompose organic pollutants in water system under visible light irradiation by rapid enrichment and efficient photodegradation.

A systematic investigation of the bio-toxicity of core-shell magnetic mesoporous silica microspheres using zebrafish model

In this work, shearing interface coassembly in biliquid phase systems is employed to synthesize biocompatible core-shell magnetic mesoporous silica microspheres with uniform size of about 600 nm, perpendicular mesopores of 6.0 nm and large pore volume of 0.77 cm3/g. The toxicology assays based on the zebrafish model was conducted to test under a high-throughput manner for the biosafety of Fe3O4@RF@mSiO2 microspheres. The highest no observed toxic effect concentration (NOEC) estimated by the acute toxicity assay for the microspheres was 1.6 mg/mL. The estimated number (measured by ICP-MS) of the penetrated microspheres at this concentration was 2 × 106 per embryo. The results of three different performed toxicity assays show no overall acute toxicity, teratogenicity, or neurotoxicity of the microspheres on zebrafish embryos at any of the tested concentrations (from 0.1 to 1.6 mg/mL) via its multifunctional microstructure. Here, gemcitabine (GEM) as a model of anti-cancer drug was loaded into the mesopores of Fe3O4@RF@mSiO2 microspheres to study their drug release behavior. The microspheres were found to exhibit pH responsive property, which benefits for the GEM release under cancer therapy. Overall, this study offers promising avenue for effective evaluation of magnetic core-shell microspheres for drug delivery and cancer therapy applications.

Plasmolysis-Inspired Nanoengineering of Functional Yolk-Shell Microspheres with Magnetic Core and Mesoporous Silica Shell.

Yolk-shell nanomaterials with a rattle-like structure have been considered ideal carriers and nanoreactors. Traditional methods to constructing yolk-shell nanostructures mainly rely on multistep sacrificial template strategy. In this study, a facile and effective plasmolysis-inspired nanoengineering strategy is developed to controllably fabricate yolk-shell magnetic mesoporous silica microspheres via the swelling-shrinkage of resorcinol-formaldehyde (RF) upon soaking in or removal of n-hexane. Using Fe3O4@RF microspheres as seeds, surfactant-silica mesostructured composite is deposited on the swelled seeds through the multicomponent interface coassembly, followed by solvent extraction to remove surfactant and simultaneously induce shrinkage of RF shell. The obtained yolk-shell microspheres (Fe3O4@RF@void@mSiO2) possess a high magnetization of 40.3 emu/g, high surface area (439 m2/g), radially aligned mesopores (5.4 nm) in the outer shell, tunable middle hollow space (472-638 nm in diameter), and a superparamagnetic core. This simple method allows a simultaneous encapsulation of Au nanoparticles into the hollow space during synthesis, and it leads to spherical Fe3O4@RF@void-Au@mSiO2 magnetic nanocatalysts, which show excellent catalysis efficiency for hydrogenation of 4-nitrophenol by NaBH4 with a high conversion rate (98%) and magnetic recycling stability.

Pyrophosphate‐Imprinted Magnetic Mesoporous Silica Microspheres for Recognition, Enrichment and MS Detection of Phosphopeptides

AbstractProtein phosphorylation is one of the most important post‐translational modifications involved in the regulation of various cellular processes. Selective capture of phosphopeptides from complex biological samples is essential prior to mass spectrometry (MS) analysis of protein phosphorylation. Herein, pyrophosphate‐imprinted magnetic affinity microspheres composed of a magnetic Fe3O4 core and pyrophosphate‐imprinted mesoporous silica shell have been facilely synthesized through the combination of a surfactant‐templated sol–gel process and a dual‐template docking oriented molecular imprinting process. They have a relatively large specific surface area (142.1 m2 g−1) and superparamagnetism with a saturation magnetization (Ms) value (66.54 emu g−1). In virtue of their selective affinity with molecular recognition, porous structure and strong magnetism, they can realize the rapid and effective purification of phosphopeptides from real biosamples for MS analysis. These results reveal that this work will be beneficial for future applications in the fast magnetic isolation and specific identification of phosphorylated biomolecules.