Magnesium In Human Serum Research Articles

Simple SPR-based colorimetric sensor to differentiate Mg2+ and Ca2+ in aqueous solutions

L-tryptophan functionalized AgNPs were successfully fabricated using a one-pot synthesis method and assessed as a colorimetric probe for rapid and accurate determination of Mg2+ ions. The developed sensor showed a selective response towards Mg2+ with no interference from Ca2+ in the wide concentration range of 1–200 µM. The sensor's response was optimized in the pH range of 9–10, which can be attributed to the protonation of amine groups and their interaction with Mg2+ ions. The stability and selectivity of the sensor were examined in different salt (NaCl) and other metal ions, respectively. The L-tryptophan-AgNPs sensor detected Mg2+ with the limit of detection of 3 µM, which is way lower than the concentration range of magnesium in human serum (0.75–1.05 mM). The recovery values of the developed sensor were in the range of 96–102% for the determination of Mg2+ in urine samples. The obtained performances proved the potential application of the developed sensor for clinical diagnostic of Mg2+ ions where an accurate and rapid response is needed.

Development of high accuracy methods for the certification of calcium, iron, magnesium and potassium in human serum

High accuracy methods were developed for the measurements of calcium, potassium, iron and magnesium in human serum using standard addition and isotope dilution mass spectrometry methods. The results were comparable to those obtained by other national metrology institutes and designated institutes as demonstrated in an inter-laboratory comparison. The methods were then adopted for the assignment of reference values in a human serum material and the uncertainties associated with the certified values were obtained by combining the uncertainty components from the characterisation, homogeneity and long-term stability of the materials. The certified values are traceable to the International System of Units and the material can be used by the clinical testing laboratories to validate their methods or as a quality control material to improve the accuracy of their measurements.

The Application of Partial Least Squares Method (PLS) for Simultaneous Spectrophotometric Determination of Calcium and Magnesium in Human Serum

Summary Application of a partial least squares algorithm (PLS-1) is proposed in this work for simultaneous spectrophotometric determination of calcium and magnesium in human serum. Two chromogenic agents, Calmagite [1-(1-hydroxy-4-methyl-2-phenylazo)-2-naphtol-4-sulfonic acid] and o-Cresolphtalein [3′,3″-bis{[bis-(carboxymethyl)amino]methyl}-5′5″-dimethylphenolphtalein] were used, which form color complexes with the two elements studied. As expected, significant interferences were observed for the individual methods (Ca-o-Cresolphtalein and Mg-Calmagite) in the presence of the second metal. However, applying a full-spectrum chemometric method PLS-1, it was possible to eliminate these interferences in the calibration step and satisfactory prediction results were obtained for calcium and magnesium in real serum samples. The proposed procedure was validated using Refererence Serum (171760 Boehringer Mannheim, Germany). The analytical results obtained for human serum were compared with the results of AAS determination.

Ion chromatography as potential reference methodology for the determination of total calcium and magnesium in human serum.

The potential of ion chromatography to serve as a new reference method principle for the determination of total calcium and magnesium in human serum was investigated. Sample pretreatment consisted of acidic dilution and filtration. Detection was based on conductivity. Mean total coefficients of variation for calcium and magnesium were respectively 0.91% and 1.20% and the mean method bias was +0.06% for calcium and -0.35% for magnesium, as determined with five control materials from the Community Bureau of Reference and the National Institute of Standards and Technology. These results were comparable to those of the proposed candidate reference methods based on flame atomic absorption spectrometry. Therefore, we consider ion chromatography as a valuable reference methodology for the determination of total calcium and magnesium in human serum.

A flow injection analysis system involving immobilized NADH oxidase in column form for clinical analysis

A highly sensitive FIA system for chemiluminometric determination of reduced coenzyme, NADH, was developed, using immobilized NADH oxidase from Brevibacterium ammoniagenes. The enzyme catalyzed the oxidation of NADH generating hydrogen peroxide which emitted chemiluminescence when mixed with luminol and potassium ferricyanide. The immobilized enzyme reactor was a mini-column, measuring 1 or 2 mm in inner diameter and 20 mm in length, and the sample volume was only 1 μl per assay, with a feeding speed of one sample per min and a lowest detection limit of 10 pmol NADH. A FIA system was also developed for the determination of magnesium in human serum, using an enzyme column reactor with simultaneously coimmobilized hexokinase, D-glucose-6-phosphate dehydrogenase, and NADH oxidase. The performance of the system was as satisfactory as a routine colorimetric assay, but with much higher sensitivity.

Usefulness of glucokinase from Bacillus stearothermophilus for the enzymatic measurement of magnesium in human serum.

Usefulness of glucokinase from Bacillus stearothermophilus for the enzymatic measurement of magnesium in human serum.

Interference from citrate using the Titan yellow method and two fluorometric methods for magnesium determination in plasma

This study was undertaken to learn whether or not citrate interfered with the determination of magnesium in blood plasma by the Titan yellow method and by two fluorometric methods that employ 8-hydroxyquinoline or 8-hydroxy-5-quinolinesulfonate. A series of determinations of magnesium in human serum was made by these three methods and by atomic absorption spectrophotometry, which served as a reference. The results support the conclusion that the values for magnesium analyzed in citrated plasma by these three methods represent total plasma magnesium minus the interference from citrate.

Quantitative determination of calcium and magnesium in human serum by flame spectrophotometry.

Quantitative determination of calcium and magnesium in human serum by flame spectrophotometry.

The Spectrophotometric Determination of Magnesium in Human Serum*

Journal Article The Spectrophotometric Determination of Magnesium in Human Serum Get access Patricia Craig, B.S., Patricia Craig, B.S. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan Search for other works by this author on: Oxford Academic Google Scholar Bennie Zak, B.S., Bennie Zak, B.S. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan Search for other works by this author on: Oxford Academic Google Scholar Lloyd T. Iseri, M.D., Lloyd T. Iseri, M.D. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan Search for other works by this author on: Oxford Academic Google Scholar A. J. Boyle, M.D., A. J. Boyle, M.D. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan Search for other works by this author on: Oxford Academic Google Scholar Gordon B. Myers, M.D. Gordon B. Myers, M.D. From the Departments of Chemistry and Medicine, Wayne University, Detroit, Michigan Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 21, Issue 4_ts, April 1951, Page 394, https://doi.org/10.1093/ajcp/21.4_ts.394 Published: 01 April 1951 Article history Received: 04 December 1950 Published: 01 April 1951