Macro-and Trace Elements Research Articles

Variation of macroand trace elements in organs of the blue honeysuckle subspecies of various environmental and geographic provenance when grown in the Near-Ob foreststeppe (West Siberia, Russia)

The variation limits and organ distribution of macroand trace elements were determined in plants of the three subspecies of Lonicera caerulea L. The leaves, as compared with the stems, were found to accumulate more of B, Са, Mg, Mo, P and Sr and less of Al, Mn, Ti and Zn. The plants of different subspecies, grown on soils of the similar chemical element composition, showed statistically significant differences in contents and organ distribution patterns of Al, Cr, Cu, Fe, Mg, Mo, Na, Ni, Pb, Si, Ti and Zn. The stems of the subspecies of the Far East provenance (L. сaerulea subsp. kamtschatica and L. сaerulea subsp. venulosa) had abnormally high contents of Al, Cr, Fe, Na, Si and Ti.

The effect of soil properties and climate on phytocoenoses biodiversity in the forest-steppe zone of West Siberia

The effect of weather conditions and such soil properties as pH, salt content, as well as different forms of macroand trace elements, on phytocoenoses biodiversity and chemical element contents in the aboveground phytomass was investigated in the forest-steppe zone of the West Siberian plain. Soil chemical element composition and salinity content were found to affect the phytomass content of N, P, K, Na, Ca, Mg, Fe, Mn, Zn, Cu, Sr, Li.

МОРФОЛОГИЧЕСКИЙ СТАТУС КРОВИ БЫКОВ-ПРОИЗВОДИТЕЛЕЙ ЧЕРНО-ПЕСТРОЙ ПОРОДЫ ПРИ ИСПОЛЬЗОВАНИИ МИНЕРАЛЬНОЙ ДОБАВКИ ИЗ ЖМЫХА ПАНТОВ МАРАЛА

The usage period of seed-bulls, their sexual activity, the amount and quality of semen obtained from them depend both on the individual characteristics of the animal organ-ism and numerous environmental factors. The diet should contain all the necessary organic substances: easily digest-ible carbohydrates (starch and sugar), lipids, minerals and vitamins. The lack or deficiency of macro-and trace ele-ments in the diet and inadequate ratio of individual mineral elements disrupts the processes of nutrient intake into the animal body, thereby contributing to the disruption of meta-bolic processes. However, the mineral composition of plant forages depends largely on soil and climatic conditions, production and storage techniques. The average trace element deficiency in energy-balanced diets is 30-50%, and that necessitates using mineral supplements in animal diets. The nature of changes occurring in an animal body when feeding new forages, feed supplements, biological growth promoters and other products should and may be controlled through the main blood values. The research goal was to study the blood morphological composition of Black Pied seed bulls when using a mineral supplement based on maral antler press-cake. The experimental stud-ies were carried out in the breeding company AO “Plempredpriyatiye Barnaulskoye”, the Altai Region, in 2021, in the control and trial groups of comparable Black Pied seed bulls used on the farms of the Altai Region. Along with the basic diet, the seed bulls of the trial group received the mineral supplement based on maral antler press-cake; the daily dose -15.0 g per head, for 30 days with 10 days’ interval aftereach application. The supple-ment was mixed with compound feed before feeding. The mineral supplement was made at the Federal Altai Scien-tific Center of Agro-Biotechnologiesafter fermentation and autoclaving of maral antler press-cake; a significant amount of biologically active substances was found in ant-ler press-cake. The morphological status of the blood of seed bulls when using the mineral supplement made from maral antlers was characterized by a higher counts of erythrocytes, hemoglobin and leukocytes by 8.8%, 8.31% and 3.6%, higher values of hematocrit volume, mean cell hemoglobin (MCH), mean corpuscular volume (MCV), and mean cell hemoglobin concentration (MCHC) by 9.78%, 0.3%, 1.8% and 1.4%, respectively, as compared to those blood indices of the control group animals; that was indica-tive of its hematopoietic effect.

Some aspects of the occurrence and development of chronic aseptic pododermatitis in highly pregnant cows

Pododermatitis in tall cows that occur on the background of secondary osteodystrophy can lead to significant economic losses, consisting of forced culling of animals, reduced milk yield, reproductive function and not enough receiving of calves. Therefore, the question of studying the peculiarities of origin and development, in particular chronic aseptic pododermatitis in these animals is quite actual. It is defined that with the same type of highly concentrated feeding, hypodynamics and insufficient insolation in the organism of highly pregnant cows, the process of assimilation of feed minerals is disrupted (develops, as is known, for dysfunction of the endocrine glands due to prolonged ketosis), which is appeared by resorption of the hoof bone, low rates of mass-volume extraction analysis of bone biopsy and testifies a systemic violation of mineral metabolism in animals and is confirmed by biochemical parameters, in particular, the concentration in the hoof horn of individual macro-and trace elements, as well as biophysical parameters of the epidermis of the hooves. Thus, the concentration of Calcium in the hoof horn is 16.8 % lower than in clinically healthy animals, Sulfur – by 20.3 %, Copper – by 16.2, Zinc – by 15.0, Cobalt – by 13.0 with increasing concentration of sulfhydryl groups by 34.3 % and moisture, respectively – by 5.5 %, which causes a decrease in the density and hardness of the epidermis of the hooves by 5.4 % and 19.0 %, respectively, and contributes to the deterioration of the quality of the hooves of highly pregnant cows. Such changes lead to deformation of the hooves, violation of the uniform load on their soles and the development of aseptic inflammation of the skin base of the hooves of a chronic nature, because the process of metabolic disorders occurs over a long period of time.

Macro- and trace elements content in honeybee pollen loads in relation to the harvest season.

The content of macro-and trace elements in honeybee pollen loads collected from the Al-Ahsa oasis, Saudi Arabia during spring, summer, autumn, and winter in 2018/2019 were determined. After critical screening and observation, we found that the major pollen floral resources were rapeseed, sunflower, summer squash, and date palm. The highest values of sodium (Na), potassium (K), calcium (Ca), magnesium (Mg), phosphorus (P) and manganese (Mn) were found in pollen loads trapped during spring and winter. The highest levels of copper (Cu) and iron (Fe) were found from pollen loads harvested during autumn, while the highest content of zinc (Zn) was found in pollen loads collected during the summer. Higher concentrations of the essential elements (Na, K, Ca, Mg and P) for human nutrition found in pollen loads collected during the spring and winter seasons, make them valuable food supplements. Finally, the elements contained in pollen loads can be related to the season of pollen load collection.

ELEMENT STATUS OF THE PEDIATRIC POPULATION OF TECHNOGENIC GEOCHEMICAL AREAS

Introduction. The Ural part of the Republic of Bashkortostan is distinguished by a variety of geomorphological, geological and geographical factors, economic specialization and level of economic development. However, the degree of impact of the total anthropogenic load on the environmental state of the entire territory is classified as “strong”. The influence of living environment features on the elemental status of the population, including children, negative trends in morbidity rates necessitate biomonitoring, based on the study of the composition of the human biological media. Material and methods. Using the atomic absorption method 491 samples of children’s hair have been analyzed. Quantitative assessment of the levels of Ca, Mg, Fe, Zn, Cu, Mn, Cr, Ni, As, Pb, Cd accumulation in the hair in children aged 0 - 7 years old, born and lived in the industrial, industrial-agricultural and predominantly agricultural areas of the eastern part of Bashkiria has been done. Results. In the body of children, the accumulation of toxic metals originates from food and inhaled polluted air coming from industrial plants. Proof of inhalation exposure is the relationship between the decrease in the level of toxic elements in the emissions of town-forming enterprises and children’s bio-media over a ten-year observation period. The adaptability of children’s body to the macro-and trace elements presented in the residential zone has been revealed. Conclusions. The metal concentration in the hair of children is characterized by a very large variability, at which an inverse relationship between the accumulation of biochemically necessary and potentially dangerous elements is noted. Toxic elements penetrate through the intrauterine barrier; as a result, the child is born with elevated concentrations of heavy metals, a significant decrease of which occurs by the end of the first year of life.

Дослідження репродуктивних функцій свиноматок при згодовуванні хлорели

The introduction of intensive development of industrial pig production, above all, depends on the full feeding of animals, which, in turn, depends on the qualitative composition of feed. In addition to the main nutrients that perform the functions of plastic and energy material, in feed rations of animals requires the presence of a wide range of compounds with high biological activity - vitamins, macro-and trace elements, enzymes and many others, contained in  chlorella. Therefore the purpose of this work was to investigate the effect of chlorella as an additional source of vitamins and biologically active substances on reproductive functions and productivity of sows. The objects of the study were sows of Landras breeds at the age of 2 years and chlorella in the form of a suspension and in the form of granular mixed fodder. For research 30 sows were selected, which were placed in 3 sections with 10 heads in each of them. The standardization of feeding of sows was based on the concentration of energy and nutrients in 1 kg of full-fodder feed. To ensure full feeding of sows, the content of exchange energy and biologically active substances in the composition of feed was calculated. Sow feeding was carried out with full-feed compound twice a day in accordance with the adopted feeding scheme: 1 group (control) received a complete diet; ІІ group (experimental) - full-fodder feed with free access to drinking suspension Chlorella; group III (experimental) - granulated mixed fodder with spray suspension of chlorella. The results of current research showed the following indicators: live weight of piglets increased, on average, by 35 %, milkability increased by 24 %, and the survival of piglets was 90 % in that groups whose diet included chlorella. Also, the feeding of piglets with a suspension of chlorella contributed to a decrease in the incidence of diarrhea. The weight of one pig in experimental groups was on average 6,8 % higher than in the control group of pigs, whose diet consisted only of feed. The practical and scientific work of many researchers has shown that the use of chlorella as an additional source of valuable substances in feeding farm animals reduces the cost of their maintenance, including the cost of feed. Economic efficiency of our research has shown that the use of chlorella in sows' diets as in the composition of the feeds and suspension does not affect the formation of prime costs per 1 head. And due to an increase in live weight gain, high preservation of young animals in experimental groups, net profit of 5509 and 5831 UAH was received. Thus, the mechanism of action of chlorella suspension is determined by its effect on all systems and functions of the animal organism. Chlorella suspension is a natural probiotic, as the quality of feed increases, it does not contain fodder toxins, which affects the increase in live weight of farm animals.

Адаптивна технологія вирощування гречки з умістом в зерні CR+3 на фоні застосування органічних добрив, виготовлених за новітніми технологіями

Адаптивна технологія вирощування гречки з умістом в зерні CR+3 на фоні застосування органічних добрив, виготовлених за новітніми технологіями

Comparative study on serum levels of macro and trace elements in schizophrenia based on supervised learning methods.

The etiology and pathophysiology of schizophrenia (SCZ) remain obscure. This study explored the associations between SCZ risk and serum levels of 39 macro and trace elements (MTE). A 1:1 matched case-control study was conducted among 114 schizophrenia patients and 114 healthy controls matched by age, sex and region. Blood samples were collected to determine the concentrations of 39 MTE by ICP-AES and ICP-MS. Both supervised learning methods and classical statistical testing were used to uncover the difference of MTE levels between cases and controls. The best prediction accuracies were 99.21% achieved by support vector machines in the original feature space (without dimensionality reduction), and 98.82% achieved by Naive Bayes with dimensionality reduction. More than half of MTE were found to be significantly different between SCZ patients and the controls. The presented investigation showed that there existed remarkable differences in concentrations of MTE between SCZ patients and healthy controls. The results of this study might be useful to diagnosis and prognosis of SCZ; they also indicated other promising applications in pharmacy and nutrition. However, the results should be interpreted with caution due to limited sample size and the lack of potential confounding factors, such as alcohol, smoking, body mass index (BMI), use of antipsychotics and dietary intakes. In the future the application of the analyses will be useful in designs that have larger sample sizes.

Assessment of Mineral Deficiencies among Grazing Areas in Uasin Gishu County, Kenya

A study conducted in the major grazing areas of Uasin Gishu involved twenty-eight (28) soils, twenty-eight (28) forage and forty-two (42) serum samples collected in six divisions at different sites. The purpose of the study was to determine the macro-and trace elements in soils, forages and animal serum, and compare to the recommended standards so as to identify those that could be presenting deficiencies in the area. Soils were extracted for available macro-minerals; Sodium (Na), Potassium (K), Calcium (Ca) magnesium (Mg) and trace elements; Iron (Fe), Manganese (Mn), Copper (Cu) and Zinc (Zn; the forage samples were assayed for the same elements plus phosphorus as total concentration on dry matter (DM) basis while blood serum was analyzed for the same forage elements plus molybdenum (Mo). Atomic absorption spectrophotometer (AAS) and UV/visible spectrophotometer were used to analyze the metal elements and molybdenum respectively. Soil and Forage analysis of samples from southern region including Kesses and Ainabkoi revealed lower levels in both macro and trace elements analyzed. Serum samples from grazing areas situated in southern region revealed similar deficiencies in most minerals. The factors responsible for the variations as soil pH, forage species and cattle breed were investigated using correlation analysis.

HUMAN ELEMENTAL PORTRAIT: MORBIDITY, DEMOGRAPHY AND PROBLEM OF NATION HEALTH MANAGEMENT

The negative effects of anthropogenic factors (excessive intake of heavy metals, deficit of essential chemicals, adverse climatic and geographical conditions) contribute to reduction of health at individual and population levels. There is a relationship between the basic demographic indices of population of some regions and countries and provision with some essential macro- and trace elements. Moreover, not only the absolute population indices of chemical elements content in hair, but also the relative indices (frequency deviation from the norm) are important biomarkers of demographic status. In the article, a relationship between an exchange of macro-and trace elements and a number of demographic indices has been shown. For example, it has been shown that an increase in prevalence among the population of Fe, Al, K excessive accumulation in men and Cu, Co deficit in women with Mn imbalance at the background can be considered as a negative demographic prognostic factor. Furthermore, calcium-phosphorus metabolism as well as exchange of cobalt correlates with the level of fertility.

Simultaneous mobilization of macro- and trace elements (MTEs) and polycyclic aromatic hydrocarbon (PAH) compounds from soil with a nonionic surfactant and [ S, S]-ethylenediaminedisuccinic acid (EDDS) in admixture: PAH compounds

A laboratory study was conducted to assess the feasibility of a washing process with nonionic surfactant in combination with EDDS for the simultaneous mobilization of MTEs and PAH compounds from a field-contaminated soil. Unit processes consisting of complexometric extraction and surfactant-assisted mobilization were combined with reagent regeneration and detoxification steps to generate innocuous products. Thirty minutes of ultrasonic mixing of the soil with a combination of 20 mL L −1 surfactant suspension and a sparing quantity (2 mmol) of EDDS mobilized virtually all of the benzo[α] pyrene (B(a)P) and chrysene (Cry) and an appreciable portion of the burdens of Cd, Cr, Mn, Ni, Pb and Zn, lesser amounts of the As and Cu, but only small quantities of Al and Fe. Relative to individual reagents, combinations of surfactant (Brij98), with EDDS increased the recovery of B(a)P but seemingly did not influence Cry extraction efficiencies perceptibly. Nine sequential washes with the same initial dosage of mobilization aids decreased the quantities of both PAHs to levels in the soil that conformed to recommended maxima. What resulted was a soil that had been cleaned and a limited quantity of innocuous wash water.

Simultaneous mobilization of trace elements and polycyclic aromatic hydrocarbon (PAH) compounds from soil with a nonionic surfactant and [S,S]-EDDS in admixture: Metals

This study evaluated the efficacy of soil washing with a nonionic surfactant (Brij98) in combination with a complexing reagent (ethylenediaminedisuccinic acid, [S,S]-EDDS) for the simultaneous mobilization of macro- and trace elements (MTEs) and PAH compounds from a field-contaminated soil. Soil fractionation studies indicated that an appreciable fraction of the Al, Ca, Cu, Fe and Mn was associated with the residual fraction but that much of the other trace elements (As, Cd, Cr, Ni, Pb and Zn) might be susceptible to soil washing. Ultrasonically aided mixing of the field contaminated soil with Brij98 and a sparing quantity (2mmol) of [S,S]-EDDS, simultaneously mobilized virtually all of the benzo[α]pyrene {B(a)P} and chrysene (CRY) and appreciable quantities of the trace elements (Cd, Cr, Mn, Ni, Pb, Zn) burdens. The recovery of both PAHs and trace elements were increased from the soil organic matter (SOM)-rich soil. This report concerns the fate of MTEs during soil washing. Multiple ultra-sonically aided washes (five or nine) with the same dosage of reagents mobilized virtually all of PAHs and decreased the levels of Cd, Cr, Ni, Pb and Zn to comply with recommended maxima. By contrast, the levels of As and Cu remained excessive after the treatments.