Maceration Extraction Method Research Articles

Unlocking the Antioxidant Potential of Calendula Officinalis: A Comparative Study of Extraction Methods

Calendula officinalis which is also known as marigold is a species of plant in the daisy family that has antioxidant, antiinflammatory, cytotoxic, and antitumor properties. In this study we tried to analyze the antioxidant activity of calendula officinalis flowers extract. Three different extraction methods that are soxhlet, maceration, and ultrasonic procedures are applied to the calendula officinalis flowers and antioxidant activities for each extract is analyzed and compared. Methods: In this study, soxhlet, maceration and ultrasonic extraction methods and ethanol solvent are used to obtain calendula officinalis extracts. The antioxidant activity of the extracts with different concentration are investigated under DPPH solution. Results: DPPH radical scavenging activity for the extract obtained using soxhlet procedure is higher than the other two procedures that are maceration and ultrasonic. With increasing calendula officinalis extract concentration, it is observed that the antioxidant activity is also increased as expected. Conclusions: In conclusion, calendula officinalis flowers perform very high antioxidant property and the soxhlet method can be considered as the best method for the extraction. Further researches for the application of the extract can be studied.

Optimization of crude rotenone oil extraction from birbira plant (Milletia ferruginea) seed by Soxhlet and maceration methods

The extraction of active compounds from plant materials is one of the most critical processes in the commercial development of natural products for pharmaceutical, herbicide or pesticide production. The focus of this study was extraction of crude rotenone oil (CRO) from Birbira plant (Milletia ferruginea) seed. The extraction was done using maceration anda Soxhlet extractor using organic solvents, such as ethanol, hexane, and chloroform. For the efficient extraction of crude rotenone oil from Birbira plant seed the effect of parameters, such as extraction method, solvent type, extraction time and size of crushed Birbira seed were investigated. Based on the experimental results obtained Soxhlet extraction method was more efficient (41.6% CRO yield) compared to the maceration extraction method (25.1 % CRO yield). The extraction results for both Soxhlet and maceration methods indicated that chloroform is more efficient compared to ethanol and hexane solvents. On the other hand, the effects of crashed seed size and extraction time on the % CRO yield were also investigated. The results showed that medium size (0.1–0.35 mm) for both Soxhlet and maceration extraction was the optimum size leading to the highest % CRO yield compared to fine and coarse ground seed size. The highest % CRO yield was obtained during 4 to 5 h of Soxhlet extraction and 25 to 30 h of maceration extraction method. Therefore, the Soxhlet extraction method is the fastest and efficient method for the extraction of CRO from plant materials. The characterization of Birbira seed powder and CRO was done with FTIR and Rheology test equipment. The FTIR result revealed that CRO is composed of aliphatic, olefin, polyphenol and alcohol functional groups, in which polyphenol functional groups are the most essential flavonoid components available in crude rotenone oil.

Investigating the antiviral activity of Erigeron annuus (L.) pers extract against RSV and examining its active components

Ethnopharmacological relevanceThe plants in the genus Erigeron are known to exhibit antiviral activities, including those against the respiratory syncytial virus (RSV). In traditional medicine Erigeron annuus (L.) Pers (EA) has been used in the treatment of pulmonary diseases and acute infectious hepatitis. Aim of this studyThe aim of this study is to determine the optimum extraction method to produce the most potent anti-RSV extract, elucidate its mode and mechanisms of antiviral activity in both in vitro and in vivo models, and identify the chemical structures of the bioactive compounds. Materials and methodsThe whole plant of EA was extracted with ethyl acetate, methanol, ethanol, water, aqueous methanol (60, 80% and 100%) and aqueous ethanol (50, 75% and 95%) using maceration, reflux, and ultrasound-assisted extraction methods. The antiviral activities of the extracts were determined in vitro. The in vitro antiviral activities of the extracts were determined using Hep-2 cells. Four in vitro experiments were performed to determine the mode of antiviral activity of the most active extract, ethyl acetate fraction (EAE) of Erigeron annuus whole plant extract prepared by refluxing with 50% ethanol, by examining its ability to inactivate the virus directly, inhibit viral adsorption and penetration, inhibit viral replication and preventive effect. The effect of temperature and duration of treatment on these modes of action was also determined. The antiviral activity of the EAE was also assessed in vivo in a mouse model. The lung index, viral load, and lung tissue histology were measured. qRT-PCR and ELISA studies were performed to determine the expression of key genes (TLR-3 and TLR-4) and proteins (IL-2, IFN-γ, and TNF-α) related to RSV infection. The most active antiviral compound was isolated using chromatography techniques, and its chemical structure was identified through electrospray triple quadrupole mass spectroscopy and nuclear magnetic resonance spectroscopy. ResultsThe EAE was the most active on RSV. In vitro experiments showed that the antiviral activity of EAE is via direct inactivation, inhibition of entry, and inhibition of the proliferation of the virus. In vivo experiments showed that the EAE effectively inhibited the proliferation of RSV in the lungs and alleviated the lung tissue lesions in RSV-infected mice. The antiviral activity of the EAE is mediated by downregulating the expression of TLR3 and TLR4 in the lung, upregulating the expression of IL-2 and IFN-γ, and downregulating the expression of TNF-α. Apigenin 7-O-methylglucuronide was found to be a major bioactive compound in EAE. ConclusionsThe results of this study confirmed the antiviral activity of EA by inactivating, inhibiting the entry, and inhibiting the proliferation of RSV. The activity is mediated by regulating the immunity and inflammatory mediators. Apigenin 7-O-methylglucuronide is the bioactive compound present in EA.

Formulation and Evaluation of Herbal Face Wash

Herbal formulations have enlarging demand in the world market as a source of food, cosmetics, and medicinal purposes. The aim of this study is to formulate and evaluate the herbal face wash. There are three forms of herbal face wash i.e., gel, cream and liquid-based face wash. We have studied on liquid-based herbal face wash. Ingredients required in this formulation are extracted by the Maceration extraction method. Turmeric (Curcuma longa), Aloe Vera (Aloe barbadense Linn), Lemon (Citrus Limon) source of vitamin C, Neem (Azadirachta indica) are very helpful and do not have any side effects. All herbal ingredients are easily available in the surrounding area. All the plants that have been used in this article have better antioxidant, antimicrobial, and anti-inflammatory properties. There are a number of formulations available on the market with different variety of active pharmaceutical ingredients for the treatment of acne and others skin problems especially for face. Changing environmental conditions and many pollutants, especially UV rays, are harmful for the human body. They’re damaging our skin, so cosmetics are part of taking care of the skin and body parts. As we know that face is sensitive part of body The Neem has more medicinal properties, leaves and their chemical constituents have been demonstrated to exhibit anti-inflammatory, anti-hyperglycemic, antiulcer, anti- malaria, antifungal, antibacterial, antimutagenic, and anti-carcinogenic properties .This is a study to evaluate the dermal safety and efficacy of the herbal formulation. There was a remarkable decreasing in the oiliness of the facial skin and an associated minimization of arising facial problems. Few subjects also noticed improvements in skin complexion. The present work deals with Preparation of Extracts, preparation & evaluation of the herbal face wash. It is essential to evaluate the formulation to check their efficiency and effectiveness by using above parameters color, Oduor, appearance, consistency, Washability, PH & Spread ability. Formulation showed no lumps along with easy Washability, good Spread ability and neutral PH. This study presents the formulation and evaluation of a herbal face wash designed to meet the growing demand for natural and safe skincare products. The formulation process involved selecting a blend of herbal extracts known for their skin-friendly properties, along with appropriate surfactants, thickeners, and preservatives to ensure stability and efficacy. Physicochemical characteristics such as pH, viscosity. and foamability were evaluated to assess product quality. Additionally, sensory analysis was conducted to gauge user preferences regarding fragrance, texture, and overall experience. Microbiological tests were performed to confirm product safety. The results indicated that the formulated herbal face wash exhibited desirable properties, including balanced pH, suitable viscosity, and satisfactory foam formation. Sensory evaluations revealed positive consumer acceptance, with preferences for natural fragrance and smooth texture. Microbial studies confirmed the product's microbial safety for consumer use. Overall, the formulated herbal face wash offers a promising option for individuals seeking natural and effective skincare solutions. Acne and pimples are treated using herbal face washes. Herbal Facewash eliminates excess oil without eliminating nutrients from the skin and contains rich plant-based components like feverfew (Tanacetum parthenium) and neem. Azadirachta indica). The building blocks of finished herbal medicines are known as herbal preparations. These can be comminuted or powdered herbal ingredients as well as extracts, tinctures, and fatty oils of herbal materials. The demand for herbal formulations is rising on the global market. Establishing the herbal face wash with aqueous neem leaf, tulsi leaf, and orange peel extracts is a very commendable effort. The herbal face wash formulation was more effective than the commercial face wash. Since all of the ingredients in this composition are herbal, they are safe for the skin and trustworthy..

Studi Pustaka: Ekstraksi Pewarna Alami dari Tanaman di Indonesia

Dyes have played an important role in human life, therefore the production of dyes has continued to increase from the past until now. The use of synthetic chemical dyes has apparently caused various health and environmental problems, this is what has triggered the return to use of natural dyes. Through advances in science and technology, research related to the extraction of natural dyes in Indonesia was carried out until 31 scientific reports were found that studied this matter in the last ten years. Literature studies with secondary data have summarized the novelty of research related to the extraction of natural dyes from plants, where currently flower objects are of great interest for observational studies. Various types of flowers have potential pigments that can be converted into natural dyes. The maceration extraction method is still the choice for extracting plant bioactive substances. Recently a study on the extraction of dyes from fruit peel waste was also carried out. It is hoped that the development of information related to the extraction of natural dyes will have more opportunities to be applied in various aspects.

The Impact of Ginger Extract (Zingiber officinale Rosc.) Addition on Overrun Value, Melting Resistance, Viscosity, and Hedonic Ratings in Gelato

Ginger contains high levels of bioactive compounds and can be used in food preparations in extract form. However, improper extraction methods can damage or reduce these compounds. This study utilized the maceration extraction method to assess the impact of adding ginger extract at different concentrations on the overrun value, melting resistance, viscosity, and hedonic properties of gelato. A completely randomized design was used, with treatments including 0% (T0), 0.5% (T1), 1% (T2), and 1.5% (T3) ginger extract. Higher concentrations of ginger extract affected all parameters: overrun values ranged from 15.724% to 22.030%, melting resistance from 36.642 to 42.056 minutes, and viscosity from 10.06 to 12.76 dPas. Hedonic testing revealed that panelists preferred gelato with 0.5% ginger extract (T1). In conclusion, adding ginger extract to gelato significantly affects its overrun value, melting resistance, and viscosity, with the 0.5% concentration being most preferred by consumers.

Eremostachys laevigata Bunge responses to different extraction solvents and methods: physiological, biochemical, and antibacterial attributes

This study examined the biochemical composition of Eremostachys laevigata Bunge's aerial and underground organs using various solvents (methanol, water, N-hexane, chloroform, and acetone) and their impact on antibacterial activity against eight bacterial strains (Escherichia coli, Staphylococcus aureus, Salmonella enterica, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella dysenteriae, Proteus mirabilis, and Bacillus cereus). Soxhlet and maceration extraction methods were used, and the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using the tube dilution method. The study found that N-Hexane and water solvents had the highest total phenol content, while methanol, acetone, and chloroform had the lowest. Additionally, N-Hexane and methanol solvents showed the highest and lowest flavonoid content, respectively. The methanol extract from underground organs using the Soxhlet method exhibited the strongest antibacterial activity, whereas the acetone extract showed the weakest antimicrobial effect. Staphylococcus aureus displayed both the MIC and MBC, and Salmonella enterica showed the highest susceptibility to the well diffusion method, while both Escherichia coli and Staphylococcus aureus were highly susceptible to the disk diffusion method. The underground organs contained 6.87 mg.g-1 of phenolic compounds and 0.19 mg.g-1 of flavonoids. Two iridoid glycosides, pholoyoside and sesamoside, were separated from the methanol extract of the underground organs using reversed-phase HPLC. The research highlights E. laevigata Bunge's potential as a natural reservoir of antimicrobial compounds, with phenolic and flavonoid compounds, along with iridoid glycosides, presumed to contribute to its antibacterial properties.

Synthesis of silver nanoparticles from coffee parasite leaf extract (Scurrulla ferruginea (Roxb. ex Jack) Danser) and potential activity as an antioxidant and anticancer

This study aim is to know the results of the synthesis of silver nanoparticles from quercetin and Scurrula ferruginea (Roxb. Ex Jack) danser as well as its potential as an antioxidant and anticancer. The solvent used in the maceration extraction method in this research is the polar solvent methanol. Isolation and purification of thick extract compounds from coffee parasite leaves were carried out using liquid vacuum chromatography (CVC) and gravity column chromatography (GCC) methods. Silver (Ag) nanoparticle is made with the stirrer method at a speed of 1500 rpm for 60 minutes at a temperature of 70o C. Silver nanoparticle solution was produced and then characterized using FTIR (Fourier Transform Infra Red) and PSA (Particles Size Analyzer). PSA results analysis using extract coffee parasite size 214 nm particles, whereas using flavonoid isolate quercetin confirmed size 29 nm. FTIR showed that there was a stretching vibration of the OH group at a wave number of 3229.25 cm-1, the C=O function at the wave number 1637.87 cm-1, and the CO functional group at the wave number 1016.31 cm-1. Antioxidant test using the DPPH method was carried out in silver nanoparticle solution using extract Scurrula ferruginea (Roxb. Ex Jack) danser and with quercetin obtained an IC50 value of around 115.4 and 114.9 this result describes as moderate level of antioxidants. Even though the nanoparticle anticancer test was very weak, overall, the synthesis of silver nanoparticles using flavonoid isolates was better than using extracts from coffee parasite leaves.

Evaluating Extraction Methods for Caffeine Content in Gayo Arabica Coffee Oil through Gas Chromatography-Mass Spectroscopy

This study aims to determine physicochemical properties, and caffeine analysis of green bean coffee essential oil (GBCEO) and roasted bean coffee essential oil (RBCEO) by maceration and soxhlet extraction methods. The results indicated that RBCEO by maceration method have higher percentage of yield compared to GBCEO. By the same to soxhlet extraction method, RBCEO also showed higher percentage of yield compared to GBCEO. The refractive index of the GBCEOm and GBCEOs have a lower acid value compared to RBCEOm and RBCEOs. The specific gravity obtained for GBCEOm, RBCEOm, GBCEOs, and RBCEOs ranged from 0.87 to 0.97. The results showed that GBCEOm has the highest saponification value followed by RBCEOs. GBCEOm has the highest iodine value followed by RBCEOs, while RBCEOm and GBCEOs have a similar iodine value. The peroxide value showed that GBCEOs, and RBCEOs by soxhlet extraction method have higher peroxide value. The GC-MS analysis revealed that GBCEOm has higher caffeine followed by GBCEOs with the percentages area of 9.31% and 7.36% respectively. Meanwhile RBCEOm has lower caffeine followed by RBCEOs with the percentages area of 7.36% and 4.28% respectively. This finding showed that GBCEO shows higher caffeine compound compared with RBCEO.

Protein Concentrate from Frigate Tuna Head Waste Extract Using Methanol-Acetone Solvent

The frigate tuna (Euthynnus affinis) is known for its high protein content in the head, making it suitable for the production of protein concentrate used in animal feed. This research aims to investigate the influence of adding a methanol-acetone mixture solvent, the duration of the extraction process, and the protein content in the resulting concentrate using the Kjeldahl method. The protein concentrate is produced through the maceration extraction method, where 30 grams of the frigate tuna head sample is mixed with a methanol-acetone solvent in various ratios (1:9; 3:7; 5:5; 7:3; 9:1) at a temperature of 50 °C and a stirring speed of 500 rpm, with extraction times ranging from 2 to 6 hours. Subsequently, filtration is performed, and the precipitate is dried using an oven at 100 °C for 30 minutes. The dried sample is then subjected to protein content testing using the Kjeldahl method. Research results indicate that both protein content and extraction yield values increase with the duration of the extraction process, while the water content decreases. The optimal result in maceration extraction is achieved with the methanol-acetone mixture (9:1) treatment and a 6-hour extraction time, yielding a protein content of 89.15 %, water content of 5.57 %, and an extraction yield of 23.86 %.

Phytochemical Study and Antioxidant Potential of Three Leaf Extracts of Cannabis sativa L from the Al-Hoceima Region, Morocco

Within Morocco's efforts to increase the value of cannabis, we investigated the 'Beldeya' cannabis sativa L plant, which is produced in the Ketama area of northern Morocco. In this investigation, we performed phytochemical screening and quantitative analyses of ethanol, chloroform, and hexane extracts from cannabis leaves. Additionally, we explored the antioxidant potential using two extraction methods: Maceration and Soxhlet extraction. According to the results of the phytochemical screening, most metabolites were present in the extracts, except for alkaloids and anthraquinones which were not detected in any of the extracts. We employed standard methods for the quantitative measurement of total phenol (TPC) and flavonoid content (TFC), and the results showed that the TPC and TFC were abundantly present in the hexane extract using both maceration and Soxhlet extraction methods. Antioxidant potential was assessed through the Assay for reducing 2,2-diphenyl-1-picrylhydrazyl(DPPH). The findings demonstrate that the ethanolic extract shown greater activity for both extraction techniques, displaying an IC50 value of 222.96 μg/ml for Soxhlet extraction and 256.21 μg/ml for the maceration method.

Maserasyon, refluks ve ultrasonik destekli ekstraksiyon yöntemleri ile üretilen kestane propolis ekstraktlarının farklı gıda modellerinde kullanımı: Fizikokimyasal, duyusal ve biyoaktif niteliklere etkisi

This study obtained ethanol-extracted propolis by applying maceration, reflux, and ultrasound-assisted extraction methods to propolis obtained from chestnut honey hives. Chestnut propolis extracts prepared by the methods were added to flower and chestnut honey at four different rates (0, 1, 2 and 4%) as a food model without heat treatment, and antioxidant activity, total phenolic content, colour, and sensory analysis of honey mixtures were made. Although the antioxidant activity (AO) (82.96%) and total phenolic content (TPC) (412.50 µg GAE /g) of chestnut honey were higher than that of flower honey (AO: 49.69%, TPC: 127.50 µg GAE/g), it was determined that the bioactive properties of flower honey (AO: 90.00%, TPC:713.60 µg GAE/g) rose close level to that of chestnut honey (AO:89.08%, TPC: 849.17 µg GAE/g) thanks to the addition of 4% propolis extract. Because the colour of chestnut honey with high colour intensity (1803.5 mAU) is significantly darker than flower honey (378.5 mAU), the different addition rates and extraction method of propolis did not significantly affect the final product colour intensity of chestnut honey (p>0.05), the addition of propolis in flower honey increased the colour intensity significantly (p<0.05). When propolis extracts prepared with three extraction methods are added to the basic cake formulation as a heat-treated food model in 4 different ratios (0, 1, 2 and 4%), the bioactive properties of the products (AO: 11.97%, TFM: 285.0 µg GAE/g) were determined to increase depending on the increasing propolis addition rate. Although propolis increases the antioxidant activity and phenolic substance content of the food models to which it is added due to its rich bioactive content, sensory properties of the food models were analysed due to propolis extracts' unique, intense taste, colour and flavor, and the results were evaluated with Principal Component Analysis. In terms of both food models, the products with propolis produced by maceration extraction had higher sensory evaluation scores. In contrast, the control samples without propolis were found to be similar to the products with 1% and 2% propolis added, respectively. The products with 4% propolis added showed separate clustering with low evaluation scores.

Bioactive Compounds of Sanrego (Lunasia Amara Blanco.) Extracted Using Different Methods: A Review

The use of plants as medicine has been trusted by the community since time immemorial. Sanrego (Lunasia amara Blanco.) is one plant that has been believed to be an aphrodisiac. Sanrego is a wild plant found in the forests of Sulawesi, Java, Borneo, Maluku, Philippines, Papua New Guinea and Australia, and belongs to the Rutaceae Family. Sanrego has several properties such as treating stomach pain, anti-inflammatory, anti-cancer, snake bites and increasing sexual aggressiveness. This is because sanrego has bioactive ingredients as pharmacological. Sanrego extract contains secondary metabolites or bioactive compounds that include flavanoids, tannins, alakloids, steroids and saponins. Given the large variety of bioactive compounds in a sanrego plant, it is necessary to pay attention to extraction techniques in filtering these compounds. Some environmentally friendly extraction methods that can be used include maceration extraction methods, soxhlet, reflux, ultasonic, microwave, and enzyme assistance. This review discusses and describes the various extraction techniques used to obtain plant bioactive compounds. These extraction techniques include conventional extraction techniques and non-conventional extraction techniques. This review analyzes the advantages and disadvantages of this extraction technique. This review covers the application of extraction methods to sanrego plants.

OPTIMASI GEL EKSTRAK DAUN BELIMBING WULUH (Averrhoa bilimbi Linn) SEBAGAI ANTIBAKTERI Staphylococcus aureus

Belimbing wuluh leaves (DBW) are a plant that has antibacterial properties with active flavonoid compounds. The leaf extract with a concentration of 10% has a strong inhibitory ability against Staphylococcus aureus (SA) bacteria. Gel preparations have several advantages, including having water content with cooling, soothing, moisturizing properties, easy to use, easy to penetrate the skin, and provide a healing effect. The aim of this research was to determine the optimal comparison of CMC-Na and Carbopol 940 bases in starfruit leaf extract gel preparations using the Simplex lattice design (SLD) method and to evaluate the antibacterial activity on SA bacteria. DBW extraction uses a maceration extraction method using 96% ethanol. The optimal DBW extract gel formula was determined using the Simplex Lattice Design (SLD) method using Design Expert 11. Verification of the optimal DBW extract gel formula using one sample T-test. The antibacterial test on SA used the well method and the results were analyzed using One Way Anova. The optimal formula obtained was tested for stability using freeze thaw cycling and acceptability tested. The optimal concentration of CMC-Na and Carbopol 940 produced by the SLD method is CMC-Na of 35% (0.7 g) and Carbopol 940 of 65% (1.3 g) with a total base weight of 2g. The results of the antibacterial test showed that the gel preparation could inhibit SA bacteria with an inhibition zone of 14.43 ± 0.707 mm. The results of the optimal formula verification test obtained a p value of >0.05, so the data obtained was not significantly different and it can be said that the formula for the SLD method results was valid including pH, viscosity, spreadability, adhesion power. The optimal gel formula obtained shows that the preparation is stable in storage and can be accepted by respondents.

The Effect of Extraction Method on Total Flavonoid Content of Ageratum conyzoides Ethanol Extract

This research is a type of comparative research using a quantitative approach. The study population was the population of Bandotan plants in the Kudus and Jepara regions. The organ parts taken from the plant were leaves, while the stems, roots and flowers were not extracted in this study. The extraction method used maceration and soxhletation with 96% ethanol solvent. Furthermore, to determine the total flavonoid content of <em>Ageratum conyzoides </em>leaf extraction results using the UV Vis Spectrophotometric method, it is known that <em>Ageratum conyzoides </em>leaf extract using the Soxhletation method has a higher total flavonoid content (0.32% b/v) compared to the Maceration method (0.22% b/v). The results of the total flavonoid test were then analyzed using the <em>Mann Whitney U Test. </em>Based on the <em>Mann Whitney U Test </em>shows that the value of Asymp. Sig. (2- tailed) of 0.102 > 0.05. Therefore, it can be concluded that there is no significant difference between maceration and soxhletation extraction methods on total flavonoids of <em>Ageratum conyzoides </em>extract.

Comparative study of bioactive compound content and antioxidant activity in different extraction methods of Syzygium Polyanthum leaves

Link of Video Abstract: https://youtu.be/VtCPJX9TPg4 Background : Syzygium Polyanthum is classified among the 5,000 medicinal plant species documented in the Indonesian Medicinal Herb Index. It is utilised for a variety of purposes in traditional medicine and has potential health benefits. Extraction of medicinal plants is crucial to separate active plant materials or secondary metabolites. The aim of the study was to compare extracts of Syzygium Polyanthum Leaves using different methods: maceration and UAE in extracting flavonoids, phenolic compounds and antioxidant activity. Methods: the bioactive component content and antioxidant activity of Syzygium polyanthum leaves extracted using two different methods—maceration and ultrasound-assisted extraction (UAE)—are compared. 96% ethanol was employed as the extracting solvent in the maceration and UAE extraction processes for S. polyanthum.The bioactive substances in both extracts were determined using Liquid Chromatography-High Resolution Mass Spectrometry (LC-HRMS). Result: The amount of total phenolic, flavonoid, and IC50 in S. polyanthum leaves was evaluated between the maceration extraction method and UAE. Liquid chromatography-high Resolution Mass Spectrometry (LC-HRMS) analysis was utilized to detect the presence of bioactive compounds, then classified according to the PubChem database's taxonomy. There were comparable amounts of flavonoids in both extracts (60.194 mg QE/g and 60.074 mg QE/g). According to the DPPH assay, the total phenol for the extracts obtained by maceration and UAE was 94.63 ± 2.18 mg GAE/g and 122.87 ± 5.99 mg GAE/g extracts respectively, and the IC50 for the extracts obtained by maceration and UAE were 88.21 ± 1.38 μg/mL and 41.23 ± 6.11 μg/mL. Conclusion: The findings of the study indicated that the ultrasonic-assisted extraction (UAE) approach yielded significantly greater quantities of total phenolic and flavonoid compounds, as well as exhibited stronger antioxidant activity, when compared to the maceration extraction method. The utilisation of the UAE approach resulted in a significant rise in the antioxidant activity of S. polyanthum leaves.

IDENTIFICATION AND DETERMINATION OF SAPONIN CONTENT FROM EXTRACT OF Embelia borneensis BARK

Abstract Embelia borneensis known sekilang used by the Dayak Kenyah tribe of Long Temuyat Village, Malinau, North Kalimantan as a fish poison and leech repellent. Sekilang bark extract contains alkaloids, tannins, saponins and flavonoids compounds. The purpose of this study was to determine the type and content of saponins in the methanol extract based on different maceration, reflux, and Soxhlet extraction methods. Saponin content was determined by the gravimetric method. The results showed that the type of saponin contained in sekilang bark extract was triterpenoid saponin. The average saponin content with maceration method was 24.87%, reflux method was 28.97 and soxhlation method was 29.99%. The results of data analysis with one-way anova statistical test showed that there was a significant difference between extraction methods on saponin content of sekilang stem bark with sig value of 0.009 with a significant level of 0.05 (5%).

Assessment of the In Vitro Effects of Six Selected Plant Extracts on Xanthine Oxidase

Background: Gouty arthritis is one of the most prevalent forms of arthritis caused by the deposition of uric acids mostly in joints, due to hyperuricemia. Xanthine oxidase (XO) is a key enzyme of the purine degradation pathway, catalysing the oxidation of hypoxanthine to xanthine and further to uric acid; increased activity leads to hyperuricemia hence a need to control XO activity. Commonly used therapeutic approach is the use of XO inhibitors. This study aimed to investigate if Vitis vinifera, Malus sylvestris fruit peels, Cymbopogon citratum leaves, Mucuna Pruriens leaves, Zanha africana roots and leaves, Mimosa pudica roots extracts have a Xanthine Oxidase inhibitory potential in vitro. Methodology: Air dried samples were crushed into powder using a mortar and pestle. Maceration and percolation extraction methods were used to obtain active secondary metabolites from plant materials at 10%w/v. Spectrophotometric assays methods were used for Xanthine Oxidase activity and inhibition studies. Results: The results of the current study showed that all aqueous plant extracts showed the presence of flavonoids. The experimental data showed significant inhibitory effects on XO activity. C. citratum inhibited XO activity by an average of 71%. M. sylvestris exhibited 68%, 61% and 64% at 15, 30 and 45 minutes respectively. V. vinifera exhibited 75%, 66% and 77% XO inhibitory activity at 15, 30 and 45 minutes respectively. Z. Africana exhibited 73%, 65% and 74% at 15, 30 and 45 minutes respectively. M. pudica exhibited 74%, 77% and 78% XO inhibitory activity at 15, 30 and 45 minutes respectively. M. pruriens exhibited 65%, 69% and 71% XO inhibitory activity at 15, 30 and 45 minutes respectively. Conclusion: The results obtained from the current study indicates that the flavonoids present in all plant extracts under study could be potential anti-gout agents by their inhibitory activities on Xanthine Oxidase activity

Pengaruh Variasi dan Konsentrasi Pelarut Terhadap Yield Antosianin Ekstrak Bunga Telang (Clitoria ternatea L.) Sebagai Pewarna Alami

One of these resources that may be used as a source of natural dyes is the telang flower (Clitoria ternatea L). Telang flower contains anthocyanin pigments that give it a blue color so that it can be used as a natural dye that can be applied to food or drinks. The purpose of this study was to determine the anthocyanin levels in the telang flower extract using various types of solvents and solvent concentrations. The solvent variations used were acetic acid, citric acid, and tartaric acid with solvent concentrations of 0.5, 1, 1.5, and 2% (w/v). The method used in this research is the maceration extraction method with a maceration time of 25, 50, 75, and 100 minutes. The results obtained The type of acid, namely acetic acid, citric acid, and acetic acid affected the anthocyanin yield, with values of 0.4729, 0.758, and 0.785 respectively at a solvent ratio of 1: 6. Variation of solvent concentration from 0.5%, 1 %, 1.5%, and 2% (v) 2% (v) acetic acid solvent can produce a maximum anthocyanin yield of 0.254 g/5 g, citric acid is 0.317 mg/5 gram, and tartaric acid is 0.343 at a concentration of 2% ( b/v).

ANTIBACTERIAL POTENTIAL OF EGGPLANT FRUIT (Solanum melongena L) ETHANOL EXTRACT AGAINST Propionibacterium acnes BACTERIAL GROWTH

Acne is one of the skin problems that, without our realizing it, can reduce self-confidence, despair, and even depression among teenagers and young adults. The incidence of bacterial infections is increasing with the increasing cases of resistance of bacteria, such as Propionibacterium acnes, to the side effects of anti-acne drugs. Indonesia has many plants that are beneficial to humans and have properties for treating and improving health, one of which is the eggplant. The purpose of this study is to test whether eggplant has antibacterial activity against P. acnes. The eggplant was extracted using a maceration extraction method. For antibacterial activity, well diffusion agar was used with extract concentrations of 60%, 70%, 80%, and 90%. The inhibition zones of the ethanol extract of S. melongena at 60%, 70%, and 80% concentrations were 13,76 mm, 18,63 mm, and 20,79 mm, respectively, with the highest inhibition zone at 90% concentration at 20,64 mm. The ethanolic extract of S. melongena has a strong inhibitory effect on P. acnes. Keywords: antibacterial activity, Solanum melongena L, maceration, Propionibacterium acnes.