M1 Peak Research Articles

Characterization of the M1 and M2 layers in the undisturbed Martian ionosphere at a variety of solar conditions with MAVEN ROSE

We utilize data from the MAVEN Radio Occultation Science Experiment (Withers et al., 2020) - with unprecedented coverage in solar zenith angle - to isolate the effects that local time and season induce on the photochemical ionosphere of Mars around solar minimum, leading to solar maximum. 185 out of the 1228 electron density profiles of the Martian undisturbed ionosphere collected by MAVEN ROSE between July 2016 and December 2022 show a distinct M1 layer below the M2 layer. We define undisturbed here as conditions when there are no solar events or dust storms to influence the ionosphere. This allowed us to study the behavior of both the M2 and M1 peak densities and altitudes as a function of solar zenith angle, and, for the first time, to be able to separate these trends by dusk and dawn local time, as well as by southern spring and summer versus southern fall and winter. We find that the M1 layer at small SZA can occur at altitudes lower than 100 km; that the peak altitudes and densities of both the M2 and M1 layers at dawn change more with season than they do at dusk; and that the M2 peak density decreases at a faster rate than the M1 with SZA.

Abstract 1368: Novel mRNA encoding anti-PD-L1 monoclonal antibodies for cancer immunotherapy

Abstract The difficulties with antibody-based therapies stem from their steep costs, complex procedures, and contamination risks. We investigate the use of in vitro transcription (IVT) for mRNA production, presenting a novel approach to traditional antibody treatments by promoting internal protein or antibody synthesis. We aim to create innovative mRNA-based anti-PD-L1 antibodies with strong immunotherapeutic effectiveness. The study begins with mouse immunization and hybridoma generation. Immunizing mice with the glycosylated extracellular domain of PD-L1 produces mouse antibodies against human PD-L1. Hybridomas, derived from post-immunization splenocytes, yield monoclonal antibodies screened for PD-L1 binding affinity via flow cytometry. A neutralizing assay identifies 22 antibodies with similar IC50 values and shared binding epitopes. M1, chosen for glycospecificity, affinity, and neutralizing activity, undergoes additional evaluation for the expression and anti-tumor efficacy of mRNA-encoded antibodies. The expression of M1 and atezolizumab antibodies is facilitated through an mRNA-lipid nanoparticles (LNPs) delivery system. Following intravenous administration in mice, sustained serum levels of M1 (ranging from 100 to 700 μg/mL at 24 hours post-injection) are observed. Serum concentrations of M1 and atezolizumab antibody peak at the first timepoint (24 or 48 h) after mRNA-LNP injection, respectively, and then significantly decrease within the next 4 days, with a gradual decline over the next 2 weeks. Conversely, the serum concentrations of M1 or atezolizumab protein decline significantly 24 hours post-injection and consistently stay at a baseline level (t1/2 of 94 and 4 h for M1 mRNA-LNP and protein, respectively). This indicates that the injection of encoded mRNA in mice can stably and continuously express antibodies.Administering Atezolizumab mRNA-LNP through two intravenous injections leads to a significant decrease in MC38 tumor growth, exhibiting a dose-dependent effect (TGI% = 8.1, 43.1, and 62.8 for doses of 0.2, 0.6, and 1 mg/kg, respectively). A complete tumor regression was observed up to day 19 following injection with a 2 mg/kg dose of mRNA-LNP (TGI% = 90.8%). Remarkably, a 3 mg/kg dose of atezolizumab antibody demonstrates a similar reduction in tumor growth as 0.6 mg/kg of the mRNA-LNP formulation in this model. In conclusion, this research underscores the potential of LNP-formulated mRNA as a transformative approach, converting the human body into a site for the continuous, stable expression of antibodies, offering a promising alternative to traditional protein-based therapies for combating cancer. This innovative approach shows promise in overcoming the limitations associated with traditional antibody production and clinical utilization. Citation Format: Ezra Chung, Yong Sik Bong, Renxiang Chen, Michael Zhang, Steven Long, Dong Shen. Novel mRNA encoding anti-PD-L1 monoclonal antibodies for cancer immunotherapy [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 1368.

Martian M2 peak behavior in the dayside near-terminator ionosphere during interplanetary coronal mass ejections

The interplanetary coronal mass ejections (ICMEs) can pose significant impacts on the Martian ionosphere, resulting in plasma depletion, variability, and escape to space. However, the connections between the ICMEs and the associated responses of the dayside near-terminator Martian ionospheric primary peak (M2) are not well understood. The present study primarily investigates the behavior of the ionospheric peak density (Nm) and height (hm) during the passage of ICMEs using observations from the Radio Occultation Science Experiment (ROSE) aboard MAVEN spacecraft. We have selected 8 such ICMEs (during 2017–2022) at Mars from the existing catalogs and studied the ROSE electron density profiles during quiet and disturbed time (ICMEs) for identical solar zenith angle range. We observed the elevation of the M2 peak (hm ∼ 4–16 km) during disturbed time (ICMEs) with a decrease in Nm (0.41–2.8 × 1010 m−3) in comparison to the quiet time. The present study, for the first time, addressed the influence of ICMEs on the M2 peak parameters (Nm and hm). We have proposed that the development of large vertical pressure gradient and electron temperature enhancement are plausible causes for ionospheric variability. Therefore, the present study provides new insights to understand peak plasma behavior in the dayside near-terminator ionosphere during ICMEs.

M3 electron density layer in the dayside ionosphere of Mars: Analysis of MAVEN ROSE observations

We investigate the characteristics of the topside M3 layer in the Martian dayside electron density profiles obtained from the Radio Occultation Science Experiment (ROSE) onboard the MAVEN spacecraft. M3 layer is a local enhancement in the electron density above the prominent ionospheric M2 peak. ROSE measurements have a wider latitudinal and solar zenith angle (SZA) coverage as compared to the spacecraft observations used by previous studies for characterizing the M3 layer. We analyzed 179 dayside (SZA <85∘) electron density profiles from July 2016 to December 2020. The analysis show that the typical altitude of M3 layer is ∼180 (±10) km, with a density of ∼8 (±3) x 103 cm−3, and occur ∼43 (±8) km above the M2 peak. These values are consistent with those reported by an earlier study using MGS RO data. The density of M3 layer seems to be insensitive to changes in SZA in the range of 55∘ to 85∘. The increasing trend in the M3 peak altitude with increasing SZA might be indicative of M3 forming at constant pressure levels, similar to M2. In the northern hemisphere, both the M3 peak density and altitude seem insensitive to latitudinal changes. The most commonly cited reasons for the production of the M3 layer are the enhancement in the electron temperature and the converging vertical plasma motion caused by the crustal magnetic field. Our simulations using a one-dimensional photochemical model suggest that decreased dissociative recombination rate due to electron temperature enhancements may not contribute to the formation of M3 layer. We conclude that the presence of crustal magnetic field also could not wholly explain the formation of M3 layer.

Transition in Thermal History and Recurring Burial-Exhumation Cycles along Colder Thermal Gradients at the Archaean-Proterozoic Boundary: New Insights from the Western Dharwar Craton, South India

Abstract The Hole Narsipur Greenstone Belt (HGB), in the Western Dharwar Craton, South India, preserves evidence for three cycles of metamorphism spanning the Archaean–Proterozoic transition. Changes in metamorphic style across the transition are investigated using integrated petrographic, mineral chemical, metamorphic evolutionary history, conventional geothermobarometry, and phase equilibria modeling studies of rocks of diverse bulk rock composition from the belt. These rocks record signatures of three overprinting metamorphic cycles (M2 → M3 → M4 in sequence), postdating a Mesoarchaean, M1 event in the craton. The M2 cycle represents characteristic LP–HT metamorphism along a counterclockwise (CCW) metamorphic P–T path, peak metamorphism at 4–5 kbar, 515–595°C, and high thermobaric ratios (T/P ~1050–1525°C/GPa) at the M2 peak. The intermediate M3 metamorphic cycle records a prograde burial of partially cooled M2 rocks to middle-lower crustal depths, peak metamorphism at P ~7.5–10.2 kbar, T ~630–655°C, and corresponding intermediate T/P ratios (T/P ~620–875°C/GPa) and a retrograde stage, marked by combined exhumation and cooling to P ~5.3–6.4 kbar, T ~530–575°C. The resultant P–T paths of evolution show variability of clockwise (CW) paths from classical hairpin type to two-stage prograde heating segments. During the M4 metamorphism (TMax ~630–640°C at ~8.9–10.3 kbar and with lower T/P ratios at ~610–720°C/GPa), the partially exhumed M3 crust is re-buried to lower crustal depths along an intermediate thermal gradient as during the M3 metamorphic cycle. We interpret the thermal transition, tectonic thickening along a cooler thermal gradient, repeated burial-exhumation cycles in the orogenic wedge, and tectonic mixing of the three metamorphic cycles in terms of a three-stage tectonic evolution of the HGB. These in a sequence are (a) the development of a hot and thinned crust (cf. M2 cycle), (b) the transition to a thickened crustal zone tectonic domain (cf. M3 cycle), with both the domains being part of the lithospheric peel-back driven convergence setting and (c) finally continental collision tectonics (cf. M4 cycle). The pulsating nature of orogenesis at the Archaean–Proterozoic boundary deduced here distinguishes the Western Dharwar Craton from other cratonic blocks globally at the same time frame.

Cortical Sources of Respiratory Mechanosensation, Laterality, and Emotion: An MEG Study.

Airway obstruction activates mechanoreceptors that project to the cerebral cortices in humans, as evidenced by scalp encephalography recordings of cortical neuronal activation, i.e., respiratory-related evoked potential (RREP). However, neural evidence of both high spatial and temporal resolution of occlusion-elicited cortical activation in healthy individuals is lacking. In the present study, we tested our hypothesis that inspiratory mechanical stimuli elicit neural activation in cortical structures that can be recorded using magnetoencephalography (MEG). We further examined the relationship between depression and respiratory symptoms and hemispheric dominance in terms of emotional states. A total of 14 healthy nonsmoking participants completed a respiratory symptom questionnaire and a depression symptom questionnaire, followed by MEG and RREP recordings of inspiratory occlusion. Transient inspiratory occlusion of 300 ms was provided randomly every 2 to 4 breaths, and approximately 80 occlusions were collected in every study participant. Participants were required to press a button for detection when they sensed occlusion. Respiratory-related evoked fields (RREFs) and RREP peaks were identified in terms of latencies and amplitudes in the right and left hemispheres. The Wilcoxon signed-rank test was further used to examine differences in peak amplitudes between the right and left hemispheres. Our results showed that inspiratory occlusion elicited RREF M1 peaks between 80 and 100 ms after triggering. Corresponding neuromagnetic responses peaked in the sensorimotor cortex, insular cortex, lateral frontal cortex, and middle frontal cortex. Overall, the RREF M1 peak amplitude in the right insula was significantly higher than that in the left insula (p = 0.038). The RREP data also showed a trend of higher N1 peak amplitudes in the right hemisphere compared to the left (p = 0.064, one-tailed). Subgroup analysis revealed that the laterality index of sensorimotor cortex activation was significantly different between higher- and lower-depressed individuals (−0.33 vs. −0.02, respectively; p = 0.028). For subjective ratings, a significant relationship was found between an individual’s depression level and their respiratory symptoms (Spearman’s rho = 0.54, p = 0.028, one-tailed). In summary, our results demonstrated that the inspiratory occlusion paradigm is feasible to elicit an RREF M1 peak with MEG. Our imaging results showed that cortical neurons were activated in the sensorimotor, frontal, middle temporal, and insular cortices for the M1 peak. Respiratory occlusion elicited higher cortical neuronal activation in the right insula compared to the left, with a higher tendency for right laterality in the sensorimotor cortex for higher-depressed rather than lower-depressed individuals. Higher levels of depression were associated with higher levels of respiratory symptoms. Future research with a larger sample size is recommended to investigate the role of emotion and laterality in cerebral neural processing of respiratory sensation.

Interpretation of the Altitudinal Variation in the Martian Ionosphere Longitudinal Wave‐3 Structure

AbstractUsing the electron density profiles from Mars Global Surveyor Radio Occultation observations, the ionospheric longitudinal wave‐3 (WN3) structure with different frequencies is investigated. The semidiurnal component is the dominant component for the entire Mars ionosphere profile at northern high latitudes. Both diurnal and semidiurnal components manifest double peaks in altitudinal variations in amplitude, which are 5–15 km lower than the ionospheric M1 and M2 peaks. The phase generally increases with altitude and shows a rapid rate of increase around the M2 peak. To interpret the altitudinal variation in amplitude and phase, we then derive the tidal coupling equation to elucidate the impact of atmospheric tides on the Mars ionosphere. Under the assumption of photochemical equilibrium, the tidal coupling equation indicates that the log density gradient of electrons () critically modulates the ionospheric response to atmospheric tides. In the observations, a positive correlation between the profile and the amplitude of the WN3 structure at altitude is found. The solar longitude variation in the peaks also agrees with the vertical movement of the amplitude peaks of the WN3 structure along the solar longitude. The rapid phase increase corresponds to the abrupt decrease in around the M2 peak. These observational results agree with the tidal coupling equation prediction, thereby supporting that the interpretation of in modulating the altitudinal variation in the WN3 structure is reasonable.

P-T-t-d evolution of Brattnevet Peninsula, Larsemann Hills, East Antarctica

Brattnevet Peninsula, Larsemann Hills, comprises metamorphosed acidic rocks, sedimentary rocks and basic igneous suite belonging to timespan of 920-478 Ma and have experienced three phases of deformation and metamorphism. The first and second phase of deformation gave rise to moderately plunging isoclinal and open to closed folds, respectively and the third phase resulted into broad warping. M1 prograde (amphibolite) and M2 peak (granulite) and M3 retrograde (decompression) events have been inferred. In pyroxene granulite, inclusion assemblage of quartz, biotite, plagioclase and ilmenite in hornblende correspond to the relict M1 amphibolite grade event associated with D1-D2 deformation events (occurred at ~920 Ma; 538–692 °C at ~6kb). However, the presence of enstatite, diopside, quartz, biotite, ilmenite and plagioclase in pyroxene granulite and sillimanite, biotite and almandine garnet and plagioclase in metapelite indicates granulite facies during M2 event (~530 Ma; 868–920 °C at ~6.7 kb). The retrograde M3 event (500-480 Ma; ~620 °C at ~5kb) is largely depicted in pyroxene granulite and metapelite by coronal and symplectite cooling textures. The study shows a clockwise P-T path and suggests the crustal evolution of Larsemann Hills area have commenced at the wanning stages of Grenvillian Orogeny and imprinted by the Pan-African orogenic event in the terrane.

Comparisons between beam and continuum models for modelling wheel-rail impact at a singular rail surface defect

A singular rail or wheel surface irregularity, such as a squat, insulation joint or wheel flat, can cause large wheel-rail impact force. Both the magnitude and frequency content of the impact force need to be correctly modelled because they are closely related to the formation, deterioration and detection of such irregularities. In this paper, we compare two types of commonly used wheel-track interaction models for wheel-rail impact problems, i.e., a beam and a continuum finite element model. We first reveal the differences between the impact forces predicted by the two models due to a typical rail squat using a time-frequency analysis. Subsequently, we identify the causes for the differences by evaluating the effects of different model assumptions, as well as different model parameters. Results show that the impact force consists of a forced vibration peak M1 followed by free vibration related oscillations with three dominant frequencies: f1 (340 Hz), f2 (890 Hz) and f3 (1120 Hz). Compared with the continuum model, the beam model with a Hertzian contact spring overestimates the M1 peak force. The discrepancy can be reduced by using a Winkler bedding contact model. For the track model, the beam model is comparable to the continuum model up to about 800 Hz, beyond which the track damping starts to deviate. As a result, above 500 Hz, the contact forces dominate at f2 for the beam while at f3 for the continuum model. Finally, we show that the continuum model is more accurate than the beam model by comparing to field observations. The effects of stress wave propagation on the differences are also discussed.

Two‐stage Variscan metamorphism in the Canigou massif: Evidence for crustal thickening in the Pyrenees

AbstractThe Variscan metamorphism in the Pyrenees is dominantly of the low‐pressure–high‐temperature (LP‐HT) type. The relics of an earlier, Barrovian‐type metamorphism that could be related to orogenic crustal thickening are unclear and insufficiently constrained. A microstructural and petrological study of micaschists underlying an Ordovician augen orthogneiss in the core of the Canigou massif (Eastern Pyrenees, France) reveals the presence of two syntectonic metamorphic stages characterized by the crystallization of staurolite (M1) and andalusite (M2), respectively. Garnet is stable during the two metamorphic stages with a period of resorption between M1 and M2. The metamorphic assemblages M1 and M2 record similar peak temperatures of 580°C at different pressure conditions of 5.5 and 3 kbar, respectively. Using chemical zoning of garnet and calculated P–T pseudosections, a prograde P–T path is constrained with a peak pressure at ~6.5 kbar and 550°C. This P–T path, syntectonic with respect to the first foliation S1, corresponds to a cold gradient (of ~9°C/km), suggestive of crustal thickening. Resorption of garnet between M1 and M2 can be interpreted either in terms of a simple clockwise P–T path or a polymetamorphic two‐stage evolution. We argue in favour of the latter, where the medium‐pressure (Barrovian) metamorphism is followed by a period of significant erosion and crustal thinning leading to decompression and cooling. Subsequent advection of heat, probably from the mantle, leads to a new increase in temperature, coeval with the development of the main regional fabric S2. LA‐ICP‐MS U–Th–Pb dating of monazite yields a well‐defined date at c. 300 Ma. Petrological evidence indicates that monazite crystallization took place close to the M1 peak pressure conditions. However, the similarity between this age and that of the extensive magmatic event well documented in the eastern Pyrenees suggests that it probably corresponds to the age of monazite recrystallization during the M2 LP‐HT event.

Can a single pulse transcranial magnetic stimulation targeted to the motor cortex interrupt pain processing?

The modulatory role of the primary motor cortex (M1), reflected by an inhibitory effect of M1-stimulation on clinical pain, motivated us to deepen our understanding of M1’s role in pain modulation. We used Transcranial Magnetic Stimulation (TMS)-induced virtual lesion (VL) to interrupt with M1 activity during noxious heat pain. We hypothesized that TMS-VL will effect experimental pain ratings. Three VL protocols were applied consisting of single-pulse TMS to transiently interfere with right M1 activity: (1) VLM1- TMS applied to 11 subjects, 20 msec before the individual’s first pain-related M1 peak activation, as determined by source analysis (sLORETA), (2) VL-50 (N = 16; TMS applied 50 ms prior to noxious stimulus onset), and (3) VL+150 (N = 16; TMS applied 150 ms after noxious stimulus onset). Each protocol included 3 conditions ('pain-alone', ' TMS-VL', and ‘SHAM-VL’), each consisted of 30 noxious heat stimuli. Pain ratings were compared, in each protocol, for TMS-VL vs. SHAM-VL and vs. pain-alone conditions. Repeated measures analysis of variance, corrected for multiple comparisons revealed no significant differences in the pain ratings between the different conditions within each protocol. Therefore, our results from this exploratory study suggest that a single pulse TMS-induced VL that is targeted to M1 failed to interrupt experimental pain processing in the specific three stimulation timing examined here.

Numerical simulations of the influence of solar zenith angle on properties of the M1 layer of the Mars ionosphere

AbstractThe M1 layer of the Mars ionosphere is one of its most significant features, second only to the M2 layer. Observations have shown how the physical properties of this layer depend on solar zenith angle (SZA) and solar irradiance, but these trends have not yet been explored in detail by numerical simulations. Hence, the full implications of the observational findings for the M1 layer's behavior have not been established. Here we use the Boston University Mars Ionosphere Model to simulate the M1 layer over a period of 6 months. In order to adequately reproduce the SZA dependence of the observed M2 peak density, an ad hoc isothermal electron temperature profile was required. This representation was motivated by detailed energy balance calculations that predict relatively small variations in electron temperature at the M2 peak. We find several model results consistent with observations: the simulated M1 peak density is effectively proportional to Ch(SZA)−0.5, where Ch is the Chapman function; the ratio of M1 to M2 peak electron densities is independent of SZA; the simulated M1 peak altitude decreases with increasing solar irradiance; and the simulated difference in altitude between the M1 and M2 layers increases with SZA at the observed rate. Due to limitations in the assumed neutral atmosphere, the simulated increase in M1 peak altitude with increasing solar zenith angle is significantly greater than observed. In both simulations and observations, limitations in representing the width of the M1 layer prevent meaningful comparisons and connections to the neutral scale height.

Resting-state oscillatory dynamics in sensorimotor cortex in benign epilepsy with centro-temporal spikes and typical brain development.

Benign Epilepsy with Centro-Temporal Spikes (BECTS) is a common childhood epilepsy associated with deficits in several neurocognitive domains. Neurophysiological studies in BECTS often focus on centro-temporal spikes, but these correlate poorly with morphology and cognitive impairments. To better understand the neural profile of BECTS, we studied background brain oscillations, thought to be integrally involved in neural network communication, in sensorimotor areas. We used independent component analysis of temporally correlated sources on magnetoencephalography recordings to assess sensorimotor resting-state network activity in BECTS patients and typically developing controls. We also investigated the variability of oscillatory characteristics within focal primary motor cortex (M1), localized with a separate finger abduction task. We hypothesized that background oscillations would differ between patients and controls in the sensorimotor network but not elsewhere, especially in the beta band (13-30 Hz) because of its role in network communication and motor processing. The results support our hypothesis: in the sensorimotor network, patients had a greater variability in oscillatory amplitude compared to controls, whereas there was no difference in the visual network. Network measures did not correlate with age. The coefficient of variation of resting M1 peak frequency correlated negatively with age in the beta band only, and was greater than average for a number of patients. Our results point toward a "disorganized" functional sensorimotor network in BECTS, supporting a neurodevelopmental delay in sensorimotor cortex. Our findings further suggest that investigating the variability of oscillatory peak frequency may be a useful tool to investigate deficits of disorganization in neurodevelopmental disorders.

Characterization of the lower layer in the dayside Venus ionosphere and comparisons with Mars

The influence of solar zenith angle (SZA) and solar irradiance has been well characterized for the V2 layer in the Venus ionosphere, but not the V1 layer, where previous efforts were limited by data scarcity and incomplete SZA coverage. Here we use more than 200 radio occultation profiles from Venus Express with good SZA coverage to characterize how the V1 peak altitude, peak density, and morphology respond to changes in SZA and solar activity. The V1 and V2 peak altitudes vary little with SZA, and both peak electron densities vary with SZA in an approximately Chapman-like manner. These results imply that the thermal structures of the atmosphere and ionosphere between ∼125km and ∼140km vary little with SZA. As solar activity increases, the ratio of the V1 to V2 peak density increases, and the V1 morphology changes more than the V2 morphology. These results are due to the soft X-ray flux increasing relative to the EUV flux as solar activity increases. We compare the behavior of the V1 layer to the analogous M1 layer at Mars, and find that their peak altitudes respond differently to changes in SZA and solar activity. The V1 peak density also increases more with solar activity than the M1 peak density. These distinct behaviors arise from differences in their underlying neutral atmospheres.

An observational study of the influence of solar zenith angle on properties of the M1 layer of the Mars ionosphere

AbstractThe variations in peak properties of the M1 layer (the lower photochemical plasma layer) with solar zenith angle (SZA) are important relationships for understanding the physical processes which control this region of the Mars ionosphere. The behavior of the M1 layer has been poorly characterized to date. Here we introduce an automated and repeatable method for determining properties of the M1 and M2 layers simultaneously in 5600 Mars Global Surveyor radio occultation profiles of dayside electron density. The results support previous findings for M1 and M2 subsolar peak densities and the dependence of peak densities on solar zenith angle. The ratio of M1 peak density to M2 peak density remains constant at 0.4 for 70° &lt;SZA&lt; 90°, in contrast with previous numerical simulations. The M1 altitude increases with solar zenith angle with a lengthscale, L1 = 2.5 km, which is about half that of the M2 layer, L2 = 5.2 km, indicating that the two layers become increasingly separated at high solar zenith angles. The vertical width of the M1 layer, H1, decreases from 7 km to 5 km as solar zenith angle increases from 70° to 90°, whereas the vertical width of the M2 layer, H2, increases from 10 km to 14 km. The prediction of ideal Chapman theory that both the widths Hi and the lengthscales Li equal the neutral scale height is not supported by observations. These findings provide meaningful observational constraints for numerical models, which are known to have trouble reproducing observations and observed trends associated with the M1 layer.

Comparison of brain gray and white matter macromolecule resonances at 3 and 7 Tesla.

In proton MR spectra of the human brain, relatively broad macromolecule (MM) resonances underlie the narrower signals from metabolites. The purpose of this study was to quantify the MM profile in healthy human brain at 3T and 7T, both in gray matter (anterior cingulate cortex [ACC]) and white matter (centrum semiovale [CSO]). A water-suppressed, inversion-recovery pulse sequence was used to null metabolite signals and acquire MM spectra in 20 healthy volunteers using very similar methodology at both field strengths (n = 5 per region and field). The MM spectra were fitted with multiple Gaussian functions and quantified relative to the unsuppressed water signal from the same volume. MM proton concentration values were in the range of 5-20 mmol/kg. No significant differences were found between the MM proton concentration measurements by region (P ≈ 0.8) nor by field strength (P ≈ 0.5). Linewidths of the well-resolved M1 peak were slightly more than double at 7T (43.0 ± 4.7 Hz in ACC, 45.6 ± 4.1 Hz in CSO) compared with 3T (19.8 ± 3.5 Hz in ACC, 20.0 ± 4.3 Hz in CSO). The absence of differences in MM concentrations between white and gray matter implies that a single MM "baseline" may be adequate for spectral fitting of multiple brain regions when determining metabolite concentrations. Visibility of MM signals is similar at 3T and 7T.

Solar rotation effects on the Martian ionosphere

AbstractWe present a detailed investigation of the solar rotation effects on the Martian high‐latitude (~63°N–81°N) ionosphere using the electron density (Ne) data measured by Mars Global Surveyor and solar XUV and EUV fluxes measured by SOHO under high (2000–2001), medium (2003), and low (2005) solar activity conditions. A fast Fourier transform spectral analysis method is used to estimate the amplitude of the rotation period in these parameters. This method clearly reveals the presence of solar rotation effects in the Martian ionospheric Ne at all altitudes (90–220 km), peak electron density (NmM2), and total electron content under the three solar activity conditions. These effects are in phase with the solar UV fluxes (corrected for the Martian orbit). The period of rotation effect (~26 days) is the same at all altitudes, though its amplitude is strongest at the ionospheric M2 peak (~135–140 km, ~3.5–6% of the mean values) and has a secondary enhancement at the M1 peak (~110–115 km). The effect of solar rotation on the M2 peak is larger during medium solar activity (2003) than during high solar activity (2000–2001). The effect, however, is absent in the ionospheric peak height (hmM2). The rotation effects on Mars are also compared with those on the Earth. Unlike at Mars, the Earth's high‐latitude ionosphere shows no clear solar rotation effect, though the effect is observed clearly at lower latitudes.

In situ SEM studies of the transformation sequence of multistage martensitic transformations in aged Ti-50.8 at.% Ni alloys

The transformation behaviour of the multistage martensitic transformation in aged Ti-50.8 at.% Ni alloys was investigated by differential scanning calorimetry (DSC) and in situ scanning electron microscopy (SEM). The specimens aged from 673 to 748 K for 3.6 ks under an unregulated heat treatment atmosphere exhibited the double-stage transformation during cooling. The quadruple-stage transformation was observed in the specimens aged at 773 and 798 K, and the triple-stage transformation appeared in the specimen aged at 823 K under an unregulated heat treatment atmosphere. The distribution and size of Ti3Ni4 precipitates were heterogeneous in these specimens. The single-stage transformation in the specimen aged at 848 K was similar to that of the solution-treated specimen. In the forward quadruple-stage transformation, the R-phase transformation occurred in the intermediate region and around the grain boundary. The first martensitic transformation, which corresponded to the M1 peak in the DSC cooling curve, took place in the intermediate region of grains via the R phase. The second transformation, which corresponded to the M2 peak, occurred around the grain boundary via the R phase. The final transformation, which corresponded to the M3 peak, arose directly from the B2 parent phase at the grain centre. The transformation sequence and areas described above were quantitatively verified by comparing the SEM observations with the DSC measurements. The transformation sequence of the triple-stage transformation was also discussed.

Proprioceptive reaction times and long-latency reflexes in humans

The stretch of upper limb muscles results in two electromyographic (EMG) peaks, M1 and M2. The amplitude of M2 peak can generally be modified by giving prior instruction to the subject on how to react to the applied perturbation. The unresolved question is whether the amplitude modulation results from change in the gain of the reflex pathway contributing to M2, or by superposition of reaction time (RT) activity. The following study attempted to resolve this question by examining the overlap between proprioceptive RT and M2 activities. Subject's right wrist flexors were stretched, and he/she was instructed either (1) not to intervene (passive task) or (2) to react as fast as possible by simultaneously flexing both wrists (active or compensate task). Under passive and active conditions, M1 and M2 were observed from EMG of right wrist flexors, and during the active condition, RT activities were additionally observed from both sides. The onset and offset of M2 (M1(onset), M2(offset)) were measured from the passive averages, while the RT was measured from the averaged EMG response of the left wrist flexors. For between-subject correlations, the data were divided into two sets: (1) subjects with RT shorter than M2(offset) (fast group) and (2) subjects with RT more than 10ms longer than their M2(offset) (slow group). Modulation during M2 period was large for the fast group, and it was almost zero for the slow group. These results indicate that the superimposition of RT activity mainly contributes to the instruction-dependent modulation of M2 peak.

Discrete loading of the foot during hurdle drills: A pilot investigation

Lower limb function in hurdling is patently asymmetrical. The lead limb undertakes the preparatory and landing steps while the trail limb contends with the hurdle and recovery steps. Discrete loading profiles of these steps will reflect the asymmetrical function and may provide useful insight into injury mechanisms. A pilot study was undertaken to determine the loading profiles of the hurdle, landing and recovery steps of elite male hurdlers. Equivalent data for steps between hurdles, where the running action is more symmetrical, were used for the purpose of comparison, simultaneously minimising the confounding effect of speed. In-shoe pressures were recorded (FScan, 200Hz) for four elite male hurdlers while they completed a routine hurdle drill at a self-selected fast but sub-race speed. The drill comprised of three consecutive hurdles. Data for the hurdle, landing and recovery steps of the first and second hurdles, along with data for the running steps between hurdles 1 and 2, and 2 and 3, were used for the purpose of analysis. Peak pressures within 1cm2 masks were determined for the hallux, first, central and fifth metatarsals (T1, M1, M2-4 and M5 respectively). Peak pressure (kPa) and loading duration (ms) for the hurdle, landing and recovery steps are reported as a percentage of the respective limb-matched values for between-hurdle steps. For between-hurdle steps, T1, M1 and M2-4 peak pressures were 312/357, 356/306 and 362/368 kPa, lead/trail limbs respectively. For the hurdle, landing and recovery steps, pressures at T1 and M1 increased. For T1 the increases were in the order of 17%, 36% and 8% (hurdle, landing and recovery steps, respectively) while the corresponding increases at M1 were 7%, 54% and 20%. Pressures at M2-4 were similar for all steps, while M5 loaded erratically. For the between-hurdle steps, the loading durations at T1, M1 and M2-4, were 160/162, 170/142 and 190/191 ms, respectively. For the landing step, loading duration decreased for T1, M1and M2-4 (-8%, -19% and -18%, respectively). In the hurdle step, loading duration decreased for the metatarsals but not for T1. The hurdling action leads to regional pressure increases that act for shorter durations in comparison to the between-hurdle running steps. These changes are most notable at the first metatarsal, a common site of foot injury.