Nowadays, a hazardous compound such as Chromium (Cr) in groundwater is an emerging issue worldwide due to its environmental and health impacts. Membrane distillation (MD) is a promising technique to purify Chromium-contaminated groundwater (CCG). Assisted microfiltration (MF) is lodged to overcome a major problem in MD, specifically membrane fouling. In this work, a hybrid MF-MD process is proposed with fouling control incorporated into long-term operation. Derived from the results, mixed cellulose ester with 0.1 μm of pore size (MCE0.1) at 3-min ultrasonication cleaning is chosen as the optimal parameter in the MF process, because of superior flux recovery ratio (FRR) with negligible flux deficiency of approximately 10 %. Permeate flux and total suspended solids (TSS) rejection of MCE0.1 was achieved around 440 Lm−2h−1bar−1 and > 90 %, respectively. The optimized parameters in MD were selected accordingly: feed temperature of 80 °C, and feed and coolant flow rates at 4 L/min. Eventually, the long-term batch operation was executed by obtaining average flux and permeate conductivity was 7.5 LMH and around 10 μs/cm, respectively. The Cr concentration in permeate was relatively stable around 0.05 mg/L, which meets the wastewater discharge limit of 0.5 mg/L.