Lygus Populations Research Articles

Residual Effects of Novaluron and Efficacy of Subsequent Applications to Control Mid-South Tarnished Plant Bug (Hemiptera: Miridae) Populations

Novaluron is often used in the early-square development period of cotton in the midsouthern U.S. to manage immature Lygus populations. Preventing field populations of tarnished plant bug from reaching economically damaging levels is vital when protecting cotton yield. Field experiments were conducted in 2019 and 2020 to better understand impacts of initial novaluron applications, efficacy of subsequent insecticides, and residual activity of novaluron. Tarnished plant bug populations had less impact on cotton yield when an insecticide with adult activity was applied with novaluron at the third week of squaring.

Effects of Agronomic Practices on Lygus spp. (Hemiptera: Miridae) Population Dynamics in Quinoa.

Crop diversification often promotes farm sustainability. However, proper management of newly introduced crops is difficult when pests are unknown. Characterizing herbivore dynamics on new crops, and how they respond to agronomic factors, is crucial for integrated pest management. Here we explored factors affecting Lygus spp. (Hemiptera: Miridae) herbivores in quinoa crops of Washington State. Quinoa is a newly introduced crop for North America that has multiple varieties and a range of agronomic practices used for cultivation. Through arthropod surveys and discussions with growers, we determined that Lygus spp. was the most abundant insect herbivore and likely contributed to low quinoa yields in previous seasons. We assessed how different varieties (Pison and QQ74), irrigation regimes (present and not), and planting methods (direct-seeded and transplanted) affected Lygus population dynamics. Lygus phenology was correlated with timing of quinoa seed-set in July and August, corresponding to a period when quinoa is most susceptible to Lygus. Both irrigation and planting manipulations had significant effects on Lygus abundance. Irrigation reduced Lygus abundance compared with nonirrigated plots in 2018. Planting method had a significant effect on Lygus populations in both 2017 and 2018, but effects differed among years. Variety had a significant effect on Lygus abundance, but only in nonirrigated plots. Overall, our study shows that Lygus is a common insect herbivore in quinoa, and careful selection of variety, planting method, and irrigation regime may be key components of effective control in seasons where Lygus abundance is high.

A transgenic approach for controlling Lygus in cotton.

Lygus species of plant-feeding insects have emerged as economically important pests of cotton in the United States. These species are not controlled by commercial Bacillus thuringiensis (Bt) cotton varieties resulting in economic losses and increased application of insecticide. Previously, a Bt crystal protein (Cry51Aa2) was reported with insecticidal activity against Lygus spp. However, transgenic cotton plants expressing this protein did not exhibit effective protection from Lygus feeding damage. Here we employ various optimization strategies, informed in part by protein crystallography and modelling, to identify limited amino-acid substitutions in Cry51Aa2 that increase insecticidal activity towards Lygus spp. by >200-fold. Transgenic cotton expressing the variant protein, Cry51Aa2.834_16, reduce populations of Lygus spp. up to 30-fold in whole-plant caged field trials. One transgenic event, designated MON88702, has been selected for further development of cotton varieties that could potentially reduce or eliminate insecticide application for control of Lygus and the associated environmental impacts.

Volatiles from Intact and Lygus-Damaged Erigeron annuus (L.) Pers. are Highly Attractive to Ovipositing Lygus and its Parasitoid Peristenus relictus Ruthe

Trap cropping and biological control can provide a sustainable means of controlling insect pests. Insects in the genus Lygus (Hemiptera: Miridae) are major pests on cotton and horticultural crops throughout the United States, and pesticide resistance within Lygus populations necessitates more sustainable long-term management techniques. Here, we explore behavioral responses of Lygus bugs (L. rubrosignatus Knight) and an introduced parasitoid, Peristenus relictus (Hymenoptera: Braconidae), to a common field edge plant, Erigeron annuus, which has the potential to serve as a trap host. Erigeron annuus is attractive to Lygus in the field, with Lygus preferentially moving to Erigeron patches compared to more abundant cotton plants. To determine the role of odor cues in mediating this attraction, we collected volatiles from E. annuus with and without Lygus damage, and then tested the attractiveness of these volatiles vs. those of cotton to Lygus females and female P. relictus wasps using Y-tube and wind tunnel bioassays. We found that undamaged E. annuus emits high concentrations of a complex volatile blend (60+ compounds), with novel compounds induced and constitutive compounds up-regulated in response to damage. Additionally, both female Lygus bugs and female P. relictus wasps are highly attracted to E. annuus volatiles over those of cotton in almost every combination of damage treatments. Our results suggest that Erigeron annuus would be an effective trap plant to control Lygus in cotton, since it is highly attractive to both the pest and its natural enemy.

Costs of <I>Lygus</I> Herbivory on Cotton Associated With Farmer Decision-Making: An Ecoinformatics Approach

Because the farmer is typically excluded from the experimental research setting, experimental research may face challenges in evaluating pest management tactics whose costs and benefits hinge on farmer decision-making. In these cases an ecoinformatics approach, in which observational data collected from the commercial farming setting are "mined" to quantify both biological variables and farmer behavior, can complement experimentation as a useful research tool. Here I analyze such an observational data set to characterize associations between early- (June) and mid-season (July) Lygus hesperus Knight populations and farmer decisions to apply plant growth regulators and defoliants. Previous experimental work suggested the hypothesis that Lygus herbivory, by inducing abscission of young flower buds, might generate increased use of plant growth regulators and defoliants. Cotton's ability to compensate for loss of flower buds may, however, increase as plants grow. On upland cotton, June Lygus populations were associated with increased use of plant growth regulators, as expected, but this relationship was not observed for July Lygus populations. June Lygus populations were not associated with the use of defoliants, whereas, surprisingly, July Lygus populations were associated with decreases in defoliant use. In contrast to these positive and negative associations observed on upland cotton, on Pima cotton Lygus populations exhibited no associations with use of either plant growth regulators or defoliants. These results suggest that cotton responses to Lygus herbivory, as demonstrated in previously published experimental studies, can translate into economically meaningful changes in farmer decisions to apply agricultural chemicals.

Bioclimatic analyses of distributions of a parasitoidPeristenus digoneutisand its host speciesLygusspp. in Europe and North America

1 Peristenus digoneutis Loan is a parasitoid of Lygus plant bugs, which was successfully introduced from Europe into North America in the 1980s for controlling native Lygus populations. Surveys confirmed that P. digoneutis populations have become established throughout eastern North America and that the spread of the parasitoid continues. For unknown reasons, previous releases of P. digoneutis in Western Canada were not successful. 2 A bioclimate (climex®; Hearne Scientific Software Pty Ltd, Australia) model for P. digoneutis in North America was developed, based on climate and ecological parameters, and then validated with actual distribution records. The current distribution of P. digoneutis in eastern North America was consistent with the predicted distribution. The model suggests that P. digoneutis will probably continue its spread westwards throughout the U.S.A. along the Great Lakes. 3 The southern distribution of P. digoneutis is expected to be limited by hot summer temperatures, whereas its northern range is limited by the number of Lygus host generations rather than cold stress. 4 Peristenus digoneutis has the potential to occur in the southern parts of the prairie ecozone of western Canada; however, Ecoclimatic Index values in the prairies indicate mainly marginal or unfavourable conditions, which may explain why earlier releases of P. digoneutis in Western Canada failed.

Effects of Local and Landscape Factors on Population Dynamics of a Cotton Pest

BackgroundMany polyphagous pests sequentially use crops and uncultivated habitats in landscapes dominated by annual crops. As these habitats may contribute in increasing or decreasing pest density in fields of a specific crop, understanding the scale and temporal variability of source and sink effects is critical for managing landscapes to enhance pest control.Methodology/Principal FindingsWe evaluated how local and landscape characteristics affect population density of the western tarnished plant bug, Lygus hesperus (Knight), in cotton fields of the San Joaquin Valley in California. During two periods covering the main window of cotton vulnerability to Lygus attack over three years, we examined the associations between abundance of six common Lygus crops, uncultivated habitats and Lygus population density in these cotton fields. We also investigated impacts of insecticide applications in cotton fields and cotton flowering date. Consistent associations observed across periods and years involved abundances of cotton and uncultivated habitats that were negatively associated with Lygus density, and abundance of seed alfalfa and cotton flowering date that were positively associated with Lygus density. Safflower and forage alfalfa had variable effects, possibly reflecting among-year variation in crop management practices, and tomato, sugar beet and insecticide applications were rarely associated with Lygus density. Using data from the first two years, a multiple regression model including the four consistent factors successfully predicted Lygus density across cotton fields in the last year of the study.Conclusions/SignificanceOur results show that the approach developed here is appropriate to characterize and test the source and sink effects of various habitats on pest dynamics and improve the design of landscape-level pest management strategies.

Injury to Cotton by Adult <I>Lygus hesperus</I> (Hemiptera: Miridae) of Different Gender and Reproductive States

Lygus hesperus Knight (Hemiptera: Miridae) is a key pest of cotton (Gossypium spp.) in the western United States that injures floral buds (squares) and developing fruit (bolls). However, no clear relationship between Lygus population level and plant injury has been established. Age-dependent feeding activity by L. hesperus is a possible source of variation that has not been examined for its influence in studies of the impact of Lygus on cotton. Recent video-based laboratory studies indicated that feeding behaviors and trivial movement varied among L. hesperus adults of different gender and reproductive states (prereproductive; reproductive and unmated; and reproductive and mated). We compared within-plant distributions and accumulations of feeding injury to intact cotton plants corresponding to adult L. hesperus of different gender and reproductive states. Adult females, regardless of reproductive state, were observed on squares and axillary buds more often than were males. Additionally, prereproductive adults were observed on squares and axillary buds more often than were mated or unmated reproductive adults, regardless of gender. Plants that were exposed to prereproductive adults exhibited more abscised squares and more squares with injured anthers compared with plants exposed to reproductive adults. However, feeding injury did not differ by insect mating status or gender. These results are consistent with results of our previous video-based assays, and indicate adult reproductive state represents a source of variation that should be controlled in studies to evaluate Lygus-induced injury to cotton and other crop plants.

Efficacy of Beauveria bassiana Against Lygus hesperus (Hemiptera: Miridae) at Low Temperatures

The western tarnished plant bug, Lygus hesperus Knight, is susceptible to the naturally-occurring pathogen, Beauveria bassiana (Balsamo) Vuillemin, in the San Joaquin Valley of California. Recent research efforts have focused on selection of Beauveria strains that were effective against Lygus under the high-temperature conditions typical of the cotton (Gossypium spp.) production season. However, the most appropriate use of this pathogen may not be as a rescue treatment. Alternatively, B. bassiana may be useful to efforts to target overwintering populations of Lygus if isolates are available that are highly virulent under the low temperature conditions typical of winter and early-spring in the San Joaquin Valley. One commercially-available isolate and 4 native isolates of B. bassiana were assayed against L. hesperus adults under constant temperatures of 12.8, 18.3, and 23.9°C. Although decreasing temperatures were associated with diminished Beauveria-induced mortality of Lygus and slower development of disease symptoms, no differences in efficacy were detected among the tested isolates. Differences in the patterns of occurrence of Beauveria disease symptoms were observed among isolates at some temperatures, but those differences were not substantial. Furthermore, results at some temperatures suggested potential influences of Lygus adult age or gender on susceptibility to B. bassiana. Those effects should be further investigated. Overall, the results did not indicate that any of the tested isolates of B. bassiana were superior to the commercially-available isolate under low temperature conditions.

Regional Assemblages of Lygus (Heteroptera: Miridae) in Montana Canola Fields

Abstract Sweep net sampling of canola (Brassica napus L.) was conducted in 2002 and 2003 to determine Lygus (Heteroptera: Miridae) species composition and parasitism levels in four regions of Montana. Regardless of region or seasonal change, Lygus elisus (Van Duzee) was the dominant species in all canola fields sampled, averaging 60–99% of the total adult populations. Lygus borealis (Kelton), Lygus keltoni (Schwartz) and Lygus lineolaris (Palisot) were detected at much lower levels. Total lygus population density was greatest in the southwest and central regions. The northeast and southwest regions had the greatest lygus species diversity. The proportion of L. elisus increased from early to late crop maturity stages in the southwest and central regions while there was no change in northeast and southwest canola production regions. Nymphal stages III–V were dissected to detect parasitism in 2003 only. Parasitism was found to be negligible in the dissection of 1,230 nymphs. Lygus population densities in can...

EAG-Active Herbivore-Induced Plant Volatiles Modify Behavioral Responses and Host Attack by An Egg Parasitoid

Volatiles emitted by plants in response to feeding by Lygus species were tested in neurophysiological, behavioral, and parasitism trials with Anaphes iole, an egg parasitoid of Lygus. Electroantennogram analyses indicated that A. iole antennae responded to most herbivore-induced plant volatiles (HIPVs) tested and that females were usually more responsive than males. Antennal responses to (Z)-3-hexenyl acetate and methyl salicylate were among the strongest. Behavioral assays in a four-arm olfactometer demonstrated that response of female wasps to (Z)-3-hexenyl acetate varied greatly depending on preconditioning regime. Preconditioning wasps to complex host-plant odors led to stronger preference than did a single preconditioning stimulus, i.e., (Z)-3-hexenyl acetate. In a horizontal wind tunnel, female wasps were attracted by methyl salicylate and alpha-farnesene. Parasitism of Lygus lineolaris eggs by A. iole in a cotton field was greater when the eggs were associated with (Z)-3-hexenyl acetate or alpha-farnesene than with controls. Overall, the results of this study show that A. iole can perceive a variety of plant volatiles released after its host damages plants, that the degree of associative learning in A. iole can be manipulated based on preconditioning regime, and that single synthetic HIPVs are attractive to A. iole and can be used to increase attack rates on host eggs. Therefore, it appears that HIPVs have potential for use in suppression of Lygus population densities.

Native braconid parasitism of the Tarnished plant bug (Hemiptera: Miridae) in Southern Ontario

Three agricultural regions in southern Ontario (London, Niagara and Guelph) were sampled weekly from May to September for Lygus spp. and their parasitoids in 1998, 1999 and only one region in 2000. Alfalfa was the primary crop sampled, including both “clean” and weedy alfalfa fields. Lygus lineolaris (Palisot de Beauvois) accounted for 99% of all Lygus spp. collected by sweepnet. Lygus lineolaris had two generations (mid-June and late July) on alfalfa with a partial third generation in early September which contributes to the over-wintering adults. In 1998 and 2000, an early first generation peak of Lygus nymphs was observed in mid-May on the weeds, chickweed and shepherd's purse. Overall rates of parasitism (from dissections) for native parasitoids were consistent from year to year and the means of the three regions in each growing season were below 11%. Both nymphs and adult Lygus were parasitized, with the highly mobile adults being a potential means of dispersing the parasitoids. In general weedy fields were more highly parasitized than fields of weed-free alfalfa. Six species of native braconid parasitoids were collected from L. lineolaris in southern Ontario (in decreasing order of occurrence): Peristenus pallipes (Curtis), Peristenus pseudopallipes (Loan), Leiophron lygivorus (Loan), L. solidaginis Loan, L. uniformis (Gahan), and Leiophron sp. near brevipetiolatus Loan. The large populations of Lygus and the low percent baseline parasitism in southern Ontario, particularly of second generation Lygus, support the need for introduction of a multivoltine parasitoid species in this region.

A single-step multiplex PCR assay for the detection of European Peristenus spp., parasitoids of Lygus spp.

Lygus Hahn (Hemiptera: Miridae) are serious pests of agricultural and greenhouse crops throughout North America. In Europe, bivoltine Peristenus Förster (Hymenoptera: Braconidae) species have a significant impact on Lygus populations. Release and establishment of European P. digoneutis Loan in Lygus lineolaris (Palisot de Beauvois) populations in northeastern USA has renewed interest in the intended liberation of European parasitoids for Lygus control in Canada. Accurate identification of natural enemies is the cornerstone of biological control but conventional methods for identifying Peristenus species and estimating parasitism rates rely on tedious and time-consuming dissection and rearing methods. The present study describes species-specific PCR primers for three species of Peristenus, and the use of a multiplex PCR assay to detect P. digoneutis and P. stygicus Loan eggs and larvae from Lygus rugulipennis Poppius nymphs. Results indicate that the primers amplify uniquely sized, species-specific PCR products for the three species and are capable of detecting single eggs in parasitized nymphs within 3 days post-parasitism. Using a multiplex PCR assay, the primers maintain specificity and sensitivity, and allow detection of each of the three species in a single reaction. Although molecular diagnostics have previously been used in the identification of parasitoids and estimation of parasitism rates, this is the first time a single-step multiplex PCR protocol has been described.

Seasonal occurrence, species composition, and parasitism of Lygus spp. in alfalfa, canola, and mustard

AbstractField collections of over-wintering and summer adults and nymphs of Lygus lineolaris (Palisot de Beauvois) (Hemiptera: Miridae), Lygus borealis (Kelton), and Lygus elisus Van Duzee were made weekly in five fields in Saskatchewan in 1998 and 1999. The crops sampled were alfalfa, Medicago sativa L. (Leguminosae), canola, Brassica napus L. (Cruciferae), and mustard, Sinapis alba L. (Cruciferae), at Vonda, and alfalfa and canola at Saskatoon. In alfalfa, the most abundant Lygus spp. found in May and June were over-wintering adult L. lineolaris and (or) L. borealis; the predominant species in mid-June to early July was L. borealis; and the population from mid-July to late August was dominated by L. lineolaris. In canola, adult populations of Lygus spp. were not found until mid-June. The predominant species, L. lineolaris, probably over-wintering adults, was first detected in canola at the early bud stage in late June to early July; high numbers of L. lineolaris adults occurred in canola in mid-August. Populations of Lygus spp. in organic mustard were negligible. Dissections of field-collected Lygus spp. nymphs revealed parasitism in up to 70% of the midsummer population in alfalfa. In contrast, less than 1% of the late-season Lygus spp. population, primarily L. lineolaris in canola and L. lineolaris and L. borealis in alfalfa, was parasitized.

Assessment of trap crops for Lygus spp. in lettuce

The effectiveness of the use of trap crops for reducing herbivorous Lygus spp. (Heteroptera: Miridae) in a lettuce agroecosystem was evaluated in a series of field experiments in two locations in Sweden. Trap crop efficacy was determined by comparing Lygus densities in plots of lettuce with densities in adjacent plots of the cover crops Melilotus officinalis, Vicia sativa, Trifolium pratense and Medicago sativa, and the weed Artemisia vulgaris . All of the trap crops tested were more attractive to Lygus than lettuce, with between five- and 30-times as many adult Lygus on cover crops and more than 100-times more on A. vulgaris. Lygus rugulipennis was the dominant species on all plant species. These results suggest that a wide range of trap crops, including nitrogen-fixing cover crops, may be suitable for reducing populations of Lygus spp. in lettuce cropping systems.

Seasonal occurrence of Lygus bugs on field crops in Finland

A long term survey of Lygus populations (Heteroptera, Miridae) was carried out on eight arable crops in southern Finland. Sweep net samples were taken weekly during the growing season in 16 successive years. The hibernated adults first appeared in May on overwintered crops: winter rye, red clover-timothy ley and winter turnip rape. Turnip rape and ley harbored the most Lygus adults until the end of June even if some of them started to move to spring cereals (wheat, oats and barley), potato and sugarbeet after the emergence of these plants. The numbers of adults on cereals remained small until the crops reached the heading stage. The peak occurred in the first half of August. The adult numbers on potato and sugarbeet were also highest in August. The total number of adults was highest on wheat. Nymphs were found on all the studied crops. They were first captured in the second half of June and reached a peak between mid-July and mid-August. Numbers were highest on wheat. The most common species on all crops was L. rugulipennis Poppius, constituting 92% of the adults. The other Lygus species were L. gemellatus (Herrich-Schaeffer), L. pratensis (L.) and L. punctatus (Zetterstedt). A few specimens of L. wagneri Remane were also found.

Species Composition, Abundance, and Forecasting of Lygus Bugs (Heteroptera: Miridae) on Field Crops in Finland

A long-term survey of Lygus populations was carried out on 8 field crops in southern Finland. Weekly samples were taken with a sweep net throughout the growing season in 16 successive years. The dominant species on all crops was L. rugulipennis Poppius, constituting 92% of the total number of adults. L. gemellatus (Herrich-Schaeffer), L. pratensis (L.), L. punctatus (Zetterstedt), and L. wagneri Remane occurred in small numbers. Bugs were most abundant on wheat. Development of the nymphs was highly dependent on the temperature in June. The total numbers varied considerably from year to year and were gelwrally highest after warm preceding summers and dry autumns and springs. Multiregressional models were developed to forecast numbers of Lygus bugs.

Effect of annual and biennial burning of seed alfalfa (Lucerne) stubble on populations of lygus (Lygus spp.), and alfalfa plant bug (Adelphocoris lineolatus (Goeze)) and their predators1

SummaryThe effects of six burning treatments combined with two insecticide treatments of alfalfa (Medicago sativa L.) stubble on alfalfa plant bug, Adelphocoris lineolatus (Goeze) and Lygus spp. populations were evaluated over an 8‐yr period (1982–1989). The burn treatments were: burning every spring and autumn, burning in alternate springs, burning at 50–100 mm and 150–200 mm of spring growth, and an unburned control. Alfalfa plant bug, which overwinters in the egg stage in alfalfa stems, were reduced by the spring burns. First generation populations of Lygus spp., were enhanced by the burn treatments before spring growth appeared, but were reduced when alfalfa was burned over 50 mm of spring growth. Counts of the minute pirate bug, Onus tristicolor White, were enhanced in 1983 by all burn treatments, and in 1985 by some of the burn treatments. Of all the predators, only spiders were significantly reduced and only in 1986 by the burned every autumn and spring treatments.

Validation of GOSSYM: Part II. Mississippi conditions

Validation of the cotton simulation model GOSSYM was carried out with data collected from two locations during three different years in Mississippi to: (1) identify areas of the model which need to be improved to provide a universal cotton simulation model, (2) develop versions of GOSSYM with site-dependent or cultivar-dependent parameters as necessary for immediate use. Equations to calculate canopy temperatures were developed from data collected at Mississippi State Plant Science Farm, and carbohydrate partitioning logic was changed in the model giving high priority for bolls under carbohydrate stress conditions. The GOSSYM simulations were very close to real data collected during two different seasons at Mississippi State. For validating GOSSYM with data from Stoneville, Mississippi, we incorporated lygus damage into the simulated crop to give a realistic comparison with the real crop. We believe the data support a lygus population in the field plots of sufficient numbers to cause damage equal to the fruit loss we put into the simulation. Changes during this validation and the reasons for them are discussed.

SEASONAL OCCURRENCE OF LYGUS SPP. (HETEROPTERA: MIRIDAE) ON ALFALFA IN SASKATCHEWAN

Several species of Lygus (Hahn) that occur in Saskatchewan are serious and continuing pests of alfalfa grown for seed. They are readily controlled by any one of several insecticides, but the efficiency of this control is dependent upon knowing their seasonal development and occurrence on the crop.In the years 1976 and 1978, at three locations in Saskatchewan, populations of Lygus in alfalfa seed crops were sampled at 5- to 7-day intervals throughout the period of insect activity. In 1979 and 1980 samples of bugs were taken occasionally from the same locations, and in all years from other locations in the province. A sample consisted of 0–700 specimens collected by either a 38-cm sweepnet in not less than 25 and as many as 200 random sweeps, or a D-Vac sampler in not less than 3 m2 in spring and after harvest when sweep-sampling was not possible. Collected specimens were preserved in alcohol, sorted, and identified. Additional data, not previously published, were available from a similar mid-1950's study of Lygus seasonal occurrence in alfalfa seed crops in the Torch River district of northeastern Saskatchewan.