Lupinus Angustifolius Research Articles

Inactivation Efficiency of Bacillus atrophaeus Spores on Seeds of Barley, Wheat, Lupine and Rapeseed by Direct Cold Atmospheric Pressure Plasma

The objective of this study was to evaluate the antimicrobial effect of direct cold atmospheric plasma (CAPP) treatment for pre-harvest application using four different crop species: Hordeum vulgare L. (barley), Triticum aestivum L. (wheat), Brassica napus L. (rapeseed) and Lupinus angustifolius L. (lupine). The model bacterium Bacillus atrophaeus served as a proxy for spore-forming plant pathogens on the seed surface. After semi-dry inoculation of spores onto the seeds, treatment with two different plasma sources, a volume-dielectric barrier discharge and a corona discharge, and different exposure times was carried out. Subsequently, recovery of viable spores from the seeds’ surfaces was performed. Moreover, seed viability was determined based on maximum germination, as well as water contact angle as a measure for seed surface hydrophilicity. Direct CAPP treatment was efficient in reducing viable spores of B. atrophaeus with no significant differences between the plasma sources, reaching a mean inactivation of 1 log10 CFU/mL across all treatment times and crops species. Maximum germination of seeds was not negatively affected under any treatment condition. Seed hydrophilicity was increased for both plasma sources tested. Overall, this study provides valuable information on the efficiency of direct CAPP treatment of seeds with the purpose of seed hygienization with the premise of unaltered seed vitality and evaluates the potential application in comparison with previous investigated CAPP methods.

Inside Cover, Volume 141, Issue 42

The cover image is based on the Article Impact of oxidized sucrose as a bio‐based crosslinker on thermoformable films made from lupin protein isolate (Lupinus angustifolius L.) by Maximilian Maidl et al., https://doi.org/10.1002/app.56109. image

Innovative method of environmental safety research of starch‐based films with silver nanoparticles

AbstractIn this work, films based on three different starches have been developed and modified by adding silver nanoparticles. We examined the films in terms of their mechanical properties, barrier properties and environmental toxicity to plants and invertebrates. For the toxicity studies, we used the following seeds: Berny rapeseed, Brassica napus; Salvia hispanica—chia, Salvia hispanica; and Karo narrowleaf lupine, Lupinus angustifolius. The invertebrates used for toxicity studies were Daphnia pulex, Tubifex tubifex, Chaoborus sp. (larva), Chironomus aprilinus, and Artemia sp. The tests showed that KD, TD, and ZD films were the most phytotoxic, which was confirmed by the percentages of germination and growth inhibition calculated for all test plants. K2D, D2T, and Z2D films exhibited considerable toxicity to lupine and chia. The conducted biotoxicity tests concerning the modified films and their effect on the selected invertebrates showed that Daphnia pulex and Artemia were highly sensitive to the tested factors. Only Chaoborus sp. (larva) exhibited total resistance to the tested films, which did not cause death of the organism in any case. Biotests on invertebrates and plants developed and used in this research can be successfully used to determine the toxicity of other substances.

Impact of oxidized sucrose as a bio‐based crosslinker on thermoformable films made from lupin protein isolate (Lupinus angustifolius L.)

AbstractThis study investigates the effect of oxidized sucrose (OS) on selected functional properties of cast films from lupin protein isolate (LPI). LPI with a protein content larger than 0.9 g g−1 was obtained by alkaline extraction and isoelectric precipitation from bitter narrow‐leaved lupins (Lupinus angustifolius L.). OS was synthesized by vicinal diol cleavage using sodium periodate and was successfully characterized by Fourier‐transform infrared‐, 1H‐nuclear magnetic resonance spectroscopy, and aldehyde titration. Films were produced from heated (85°C, 30 min), alkaline (pH 10), aqueous solutions of LPI (0.1 mL−1), glycerol (300 mg g−1 of LPI) and OS (25, 50, 75, and 100 mg g−1 of LPI). The effect of covalent crosslinking between OS and protein chains was studied by investigating mechanical properties, moisture content, total soluble matter, water vapor permeability, and protein solubility for all films. LPI films produced with the addition of OS showed increased mechanical performance as the tensile strength was increased from 3.5 up to 9.3 MPa and elongation at break values could be raised from 118% to 176%. Taken together with the facts that these films are thermoformable and show improved wet strength compared with control films, make them promising materials for sustainable packaging and short‐term applications in agriculture and forestry.

Insights into seed coats of nine cultivars of Australian lupin: Unravelling LC-QTOF MS-based biochemical profiles, nutritional, functional, antioxidant, and antidiabetic properties together with rationalizing antidiabetic mechanism by in silico approaches

Lupins, and other legumes, have attained international interest due to their reported remarkable health benefits. Currently, the seed coats are discarded as waste or animal feed. The research presented here summarizes the potential for incorporating the seed coats into ‘whole grain’ foods. We aimed to identify metabolites found in the seed coats of nine commercial Australian cultivars of lupin (Lupinus angustifolius and L. albus species), and to evaluate and compare their functional, nutritional, antioxidant, and antidiabetic properties, along with in silico exploration of mechanisms of action for selected identified secondary metabolites. The seed coats were found to contain 79 to 90% dietary fibers and substantial quantity of essential macrometals. LC-QTOF MS-based, untargeted bioactive metabolite profiling explored a total of 673 chemical entities, and identified 63 bioactive secondary metabolites including: biophenols, unsaturated fatty acids, triterpenoids, alkaloids, and dietary prebiotics (insoluble fibers). The seed coats from these nine cultivars show substantial antioxidant activity. The cultivars of L. angustifolius inhibit α-amylase and α-glucosidase significantly in vitro. Moreover, in silico docking and dynamic simulation along with ADME/T analysis suggest that quercetin 3-methyl ether and 8-C-methylquercetin 3-methyl ether as molecules, novel in lupin seed coats, are responsible for the α-amylase and α-glucosidase inhibition. The findings indicated that lupin seed coats might be beneficial food components, rather than be discarded as ‘mill waste’

Quality and safety of products using GM soybeans in the diet of bulls

The production of high-quality and safe food products in areas contaminated by radiation due to the Chornobyl Nuclear Power Plant accident remains a pressing issue. The aim of this study was to determine the concentration and transfer factors of 137Cs, Pb, Cd, Cu, and Zn in bull products when including various high-protein feeds, such as narrow-leaved lupin and genetically modified soybean, in their diets. The study employed zootechnical methods (design and conduct of a scientific-economic experiment on animals), radiological and spectrometric methods (determination of the concentration of 137Cs, Pb, Cd, Cu, and Zn in feeds, longissimus dorsi muscle, liver, kidneys, and testes of bulls), and statistical methods. It was established that when using GM soybean in the grain mixture, the concentration of 137Cs in the muscle tissue of animals in Group II (experimental) decreased relative to the control by 2.57 Bq/kg, or by 40.5% with a statistically significant difference between groups (P > 0.99). Furthermore, the transfer factor of the radionuclide into the muscle tissue of animals in Group II compared to Group I also had 2.15% abs. lower. The content of Pb in the muscle tissue of bulls in both experimental groups exceeded the maximum permissible concentration by 2.52-2.78 times, while the concentration of Cd in the longissimus dorsi muscle and Cu in the liver of animals in Group II also exceeded sanitary-hygienic requirements by 10.0% and 2.0%, respectively. The introduction of 40% (by mass) of maize grain and 30% of GM soybean into the grain mixture instead of the same amount of wheat and lupin chop during the fattening of animals in the third zone of radioactive contamination contributed to significantly higher content and transfer of heavy metals into muscle tissue: Pb – by 10.3% and 0.10% abs., Cd – 10.0 and 0.03, Cu – 17.2 and 0.53, and Zn – by 17.2% and 2.40% abs. The results of the research can be used in agricultural enterprises and private households that produce livestock products in areas with a high anthropogenic load

ЗЕРНОПРОДУКТИВНОСТЬ И АДАПТАЦИОННЫЙ ПОТЕНЦИАЛ СОРТОВ ЛЮПИНА УЗКОЛИСТНОГО В УСЛОВИЯХ КРАСНОЯРСКОЙ ЛЕСОСТЕПИ

The aim of research is to evaluate the varieties of angustifolia lupine in terms of grain yield and adaptability parameters in the conditions of the Krasnoyarsk forest-steppe. Long-term field experiments were carried out on the experimental field of the Borsky Scientific and Production Complex of the Krasnoyarsk State Agrarian University in 2020–2022. The objects of the study are the varieties of narrow-leaved lupine: Vityaz, Belozerny 110, Belorozovy 144, Bryansky Kormovoy, Nadezhda, Siderat 46, Uzkolistny 53 and the SN 78-07 variety sample. Meteorological conditions during the years of the study varied. The 2020 growing season was warm and moisture-rich, the environmental conditions index (Ij) was 4.7. In 2021, during the main periods of growth and development, with the exception of the 3rd ten-day period of June, there was a significant moisture deficit against the background of air temperatures exceeding the climatic norm, the environmental conditions index was negative (–3.8). In 2022, uneven moisture was observed, sharp temperature changes at the beginning of plant development, the environmental conditions index was negative (–0.9). The average grain yield varied from 11.5 c/ha (variety Nadezhda) to 20.3 c/ha (variety Siderat 46). The contribution of the "vegetation conditions" factor to the formation of the lupine grain yield was 60.8 %, the contribution of the "variety" factor was 31.2 %. The varieties Belozerny 110 (bi = 1.16) and Siderat 46 (bi = 1.39) have high ecological plasticity. The highest ecological stability was noted in the varieties Siderat 46 and Uzkolistny 53, in both genotypes Sd2 = 0.02. The Vityaz variety surpasses other varieties in stress resistance. The highest genetic flexibility was demonstrated by the varieties Siderat 46, Vityaz, Belorozovy 144 and the variety sample SN 78-07. The yield range was 34.4 % (Vityaz) – 50.0 % (Nadezhda). In gene¬ral, according to the adaptive capabilities, the varieties of narrow-leaved lupine were distributed according to a decreasing trend as follows: Siderat 46 > Vityaz > SN 78-7 = Narrow-leaved 53 > Belorozovy 144 > Belozerny 110 > Nadezhda > Bryansky Kormovoy.

Effect of Fertilizers, Seeding Rates of the Legume Component and Weather Conditions on Yield and Accumulation of Plant Residues of LupineCereal Mixtures on Grain in the Center of the NonChernozem Region

On mediumcultivated mediumloamy sod-podzolic soils of the Central NonChernozem region, well provided with mobile phosphorus and potassium (IV–V class), the cultivation of lupine–cereal mixtures for grain with the participation of narrowleaved lupine varieties Ladny, spring wheat, barley and oats varieties of Nemchin breeding after grain precursors in a changing climate ensured grain production in an average of 5 up to 3.0–3.7 t/ha with a share of the legume component of 29–57% and the remaining dry mass of plant residues in the soil up to 6–8 t/ha with the accumulation of total nitrogen in it up to 40–60 kg/ha and biological – 12–17 kg/ha. In arid conditions (GTK = 0.80–0.92), a mixture of lupine and barley was distinguished for the better in terms of yield and weight of plant residues, and in case of waterlogging (GTK = 2.47) – mixtures with oats and spring wheat. The inclusion of liquid micronutrients of organic nature in the technological process with a stimulating effect for non-root top dressing eliminated the need for pre-sowing application of nitrogen fertilizer and, in conditions of moderate aridity, increased grain yield, depending on the composition of the mixture, to 3.9–4.9 t/ha (by 53–59%), the yield of plant residues – up to 7–11 t/ha (by 54–139%) and in them there is an accumulation of symbiotically bound nitrogen – up to 15–25 kg/ha or 5–8 times more than without their use.

Lupin Seed Supplementation as a Functional Feed Additive: In Vitro Ruminal Gas, Methane and Carbon Dioxide Production, Fermentation Kinetics, and Nutrient Degradability.

The inevitable enteric gas emission from ruminants is considered a modern-day problem from an environmental perspective. Addressing this problem requires nutritional approaches such as the use of phytogenic additives in ruminant diets. In this regard, lupin seed (LS) can be a useful additive due to its phytochemical constituents. Therefore, this study investigated the effects of lupin (Lupinus angustifolius) seed supplementation as a functional and sustainable feed additive in sheep diet (50:50 concentrate-to-forage ratio) on in vitro gas production (GP; mL/g DM), methane (CH4; mL/g DM) and carbon dioxide (CO2; mL/g DM) emissions, fermentation parameters, and nutrient degradability (g/kg DM incubated). Gas production and CH4 were measured per gram of incubated dry matter (DM), degradable DM (dDM), degradable neutral detergent fiber (dNDF), and degradable acid detergent fiber (dADF). Lupin seeds were included at 0 (control), 0.5, 1.0, 1.5, and 2% of the diet. The seeds contained 3.27% essential oils (DM basis), with eucalyptol as the main phytochemical. The highest GP per gram of DM and dDM was observed (p < 0.01) with 2.0% LS supplementation level. While 1.0% LS had the highest GP per gram of dNDF, 0.5% lupin diet had the highest GP per gram of dADF. Asymptotic GP and CH4 emissions linearly and quadratically increased (p < 0.01) with increasing LS level, while lag time decreased. Despite increased CH4 production, the proportion of CH4 in total biogas was lower (p = 0.008) for LS treatments than the control, with the 0.5% LS showing the lowest CH4 proportion. Production of CO2 increased with lupin seed treatments, with 0.5% LS producing the highest proportion (p = 0.027). Degradability of DM, NDF, and ADF was greater (p < 0.01) for the high LS supplementation level, while 0.5% supplementation level decreased ADF degradability. Total short-chain fatty acids, acetic acid, and propionic acid increased (p < 0.05) with LS supplementation level, leading to a reduced acetate:propionate ratio. Rumen pH decreased (p = 0.036) with LS supplementation, while ammonia-N decreased (p = 0.045) and estimated metabolizable energy increased (p < 0.001) linearly. Calculated microbial protein synthesis (p = 0.005) and gas yield (p = 0.047) increased with LS supplementation level. LS supplementation at 2.0% of diet (DM basis) increased GP and CH4 emission (mL/g DM) and enhanced nutrient degradability, suggesting its potential use as a functional feed additive for ruminants when supplemented at a 2.0% level into diet.

Diversity, abundance, and domain architecture of plant NLR proteins in Fabaceae

The resistance (R) gene family in plants is a vital component of the plant defense system, enabling host resistance against pathogens through interactions with pathogen effector proteins. These R genes often encode nucleotide-binding (NB-ARC or N) and leucine-rich-repeat (LRR or L) domains, collectively forming the NLR protein family. The NLR proteins have been widely explored in crops from Poaceae and Brassicaceae, but limited studies are available for crops in other families, including Fabaceae. To address this gap, we conducted a comprehensive genome-wide analysis of putative NLR proteins in nine Fabaceae crops, including Glycine max, Lupinus angustifolius, Medicago truncatula, Pisum sativum, Phaseolus vulgaris, Trifolium pratense, Vigna angularis, Vigna radiata, and Vigna unguiculata. Our study revealed a substantial variation in the number of NLR proteins, independent of genome size. Notably, the NB-ARC domain exhibited a preferential co-occurrence with a specific LRR domain (IPR001611) in Fabaceae. Furthermore, through protein signature analysis, we identified both species-specific and shared domains across the nine crops. By classifying the identified proteins into seven distinct classes (N, L, CN, TN, NL, CNL, and TNL), we observed species-specific clustering within the CN, TN, and CNL classes, reflecting the diversification of species within Fabaceae. This genome-wide study enhances our understanding of the NLR protein repertoire and comprehensive protein signatures in nine Fabaceae species and provides valuable insights into plant defense mechanisms.

Unraveling the dynamic interplay of microbial communities associated to Lupinus angustifolius in response to environmental and cultivation conditions

Microorganisms form dynamic communities with plants, providing benefits such as nutrient acquisition and stress resilience. Understanding how these microorganisms are affected by environmental factors such as growth conditions and soil characteristics are essential for harnessing these communities for sustainable agriculture practices and their response to climate change. The microbiome associated to Lupinus angustifolius, a legume native in Europe, with a high protein value and stress resilience was characterized for the first time. Using 16S rRNA gene and ITS amplicon sequencing, we characterized the compositional and temporal changes of the bacterial and fungal communities associated to the soil, rhizosphere, and plant compartments where Lupinus angustifolius grows naturally. Our results suggest that the main difference in the soil microbial communities is related to the edaphic properties, although environmental factors such as temperature, humidity or rainfall also influenced the composition of the soil microbial communities. We also characterized the bacterial communities associated with the rhizosphere, roots, nodules, and leaves of wild plants collected in the field and compared them against plants obtained under greenhouse conditions. In the plant compartments, the bacterial composition appeared to be more affected by the growing conditions (field vs greenhouse), than by soil characteristics or location. These results can be used to identify key taxa that may play crucial roles in the development and adaptation of the host plant and its associated microbiota to environmental changes and highlight the importance of characterizing the plant microbiomes in their natural habitats. Soil, influenced by climatic seasons, shapes the plant microbiome assembly. Lupinus recruits a core microbiome across rhizosphere, roots, nodules, and leaves, that is stable across locations. However, cultivation conditions may alter microbiome dynamics, impacting the adaptability of its components. Wild plants show a resilient and adaptable microbiome while germination and cultivation in greenhouse conditions alter its composition and vulnerability.

The Effects of Intercropping Narrowleaf Lupine with Cereals under Variable Mineral Nitrogen Fertilization

Intercropping of legumes and cereals can bring many benefits to agriculture, including an increase in yield and the quality of the crops obtained. In addition, it is possible to reduce mineral fertilization, which can have a positive impact on the environment. The aim of the field research conducted in 2021–2023 in central Poland was to evaluate the yields obtained, the content and yield of total protein and the value of land equivalent ratio in the intercropping of narrowleaf lupine with cereals at different seeding levels of components and variable mineral nitrogen fertilization. The following factors were tested: 1—share of components in the sowing: narrowleaf lupine (NL) 120 seeds m−2; spring barley (SB) 300 seeds m−2; M1—NL 30 + SB 225 seeds m−2; M2—NL 60 + SB 150 seeds m−2; M3—NL 90 + SB 75 seeds m−2; spring triticale (ST) 450 seeds m−2; M4—NL 30 + ST 340 seeds m−2; M5—NL 60 + ST 225 seeds m−2; M6—NL 90 + ST 115 seeds m−2; 2—mineral fertilization with nitrogen 0, 20, 40, 60 kg N ha−1. The most favorable results were obtained with mixtures containing 60 + 150 seeds m2 of narrowleaf lupine + spring barley and 90 + 115 seeds m−2 of narrowleaf lupine + spring triticale. In addition, the favorable results and the lack of significant differences at fertilization of 40 and 60 kg N ha−1 show the possibility of a limited dose of mineral nitrogen fertilization. Sowing narrowleaf lupine with spring barley at a ratio of 60 + 150 seeds m2 or with spring triticale at a ratio of 90 + 115 seeds m−2 and fertilizing with 40 kg N ha−1 can be recommended for agricultural practice. The proposed management technique ensures high yields of good quality and thus can be an interesting solution for sustainable cultivation and be successfully implemented on farms.

Decomposition of green manure and nutrients release in Subarctic, Kola Peninsula, Russia

In the field experiment, the intensity of decomposition and release of nutrients was studied for six types of plant residues – leaves, panicles and straw of oats (Avena sativa), as well as whole plants of lupine (Lupinus angustifolius), phacelia (Phacelia tanacetifolia) and vetch (Vicia sativa) in the arable horizon of Anthrosols on the Kola Peninsula. Samples in mesh nylon bags were sampled after 8, 12 and 24 months of exposure in soil. The loss of weight, the content of Corg and Norg, as well as ash elements – Si, Al, Fe, K, Ca, Mg, P, S and Mn were determined in all plant residue samples. As the results showed, despite the low temperatures, the decomposition of green manure in soil occurred with a high intensity, comparable to the southern chernozem zone: after a year of exposure in the soil, plant residues lost from 47% (oat straw) to 75% (lupine) of their initial ash-free weight. After 2 years, weight loss reached 68% in oat straw and 90% in lupine. The distribution of losses of Corg, Norg, and P between the types of plant residues and decomposition periods correlated significantly (at p≤0.01) with the distribution of weight losses. Changes in the content of other ash elements did not depend on weight loss: depending on the type of material and the stage of decomposition, the samples could not only lose them (K – by all plant residues; Mn – by lupine; S, Ca and Mg – by all plant residues, except straw and oat panicles), but also accumulate them in quantities exceeding the initial ones (Fe – by all species, Mn – by all species except lupine; Ca and Mg – by straw and oat panicles) at certain periods. The accumulation of elements above initial level means that the transformed plant residues can accumulate additional amounts of elements from the soil solution, while the irregular alternation of increases and decreases in Ca, Mg, S, Fe and Mn indicates the reversible nature of the process. Due to the significant differences between the types of green manure in terms of chemical composition, rate and dynamics of decomposition and release of nutrients, their use provides great opportunities for managing soil fertility and the intensity of elements cycling in agrocenoses through the species selection.

Incorporation of germinated lupin into corn-based extrudates: Focus on starch digestibility, matrix structure and physicochemical properties

The research aimed to assess the effects of incorporating germinated Lupinus angustifolius flour into corn extrudates for different periods (3, 5, and 7 days), focusing on starch digestibility, morphological structure, thermal, and pasting properties. Extrudate with germinated lupinus flour for 7 days (EG7) significantly increased the content of slowly digestible starch up to 10.56% (p < 0.05). Crystallinity increased up to 20% in extrudates with germinated flour compared to extrudates with ungerminated flour (EUG), observing changes at the molecular level by FTIR that impact the thermal and pasting properties. X-ray diffraction revealed angles of 2θ = 11.31, 16.60, 19.91, and 33.04 as a result of the germination and extrusion processes. Microstructural analysis indicated starch-protein interactions influencing changes in calorimetry, viscosity, X-ray diffraction, and digestibility. PCA allowed establishing that the addition of germinated flours significantly affected the properties and microstructural characteristics of extruded products, potentially affecting digestibility and nutritional quality.

Quinolizidine alkaloids and phomopsin A in animal feed containing lupins: co-occurrence and carry-over into veal products

Lupins are used in animal feed because of their excellent nutritional composition. Australian and European Lupinus angustifolius seeds are incorporated in compound feed of calves for veal production in Belgium. To investigate the co-occurrence of quinolizidine alkaloids (QAs) and phomopsin A (PHO A) in lupin seeds and lupin-containing feed, and the potential transfer to animal-derived foods, representative samples were obtained from various actors in the chain. A UHPLC-MS/MS method was validated for the simultaneous quantification of seven QAs and PHO A in relevant matrices. Results indicate highly consistent total QA (TQA) levels in Australian lupins (173 ± 24 mg/kg) (n = 25), while European samples showed a high variability (1442 ± 1497 mg/kg) (n = 15). PHO A was detected in 7 of 40 samples. Lupin-containing feed had a mean TQA content of 42 ± 28 mg/kg (n = 20). An in vivo feeding trial demonstrated the transfer of QAs to muscle and liver of calves that were fed the lupin-containing feed. Highest concentrations were found for lupanine in liver tissue samples (67 ± 46 µg/kg). PHO A concentrations were below the LOD in all feed and tissue samples. These results indicate that animal-derived foods (veal meat/liver) are a potential route for QAs to enter the food chain.

Use of organo-mineral amendments and Lupinus angustifolius to enhance mine tailings rehabilitation

Uncontrolled mine waste disposal globally poses severe environmental and health risks due to high concentrations of toxic substances like heavy metals and organic pollutants. This jeopardizes soil fertility and ecological balance. In this study, we adopted a novel approach based on the use of low-cost amendments that can be used in small to tailing sites for better plant growth and reducing health risks. On a 37-ha-old abandoned pyrrhotite mine site (Kettara) which was operated for 34 years near Marrakesh a high amount of tailing material low in organic C, high in salt contents, and contaminated by various heavy metals still exists. This material is low in fertility and is only patchily discovered by vegetation which should hinder the material of aeolian erosion. To increase the fertility of the material and improve vegetation growth, the soil material has to be improved by different organo-mineral amendments and their combinations as Marble waste (Mw), Clay (Cy), and Compost (Cp). To determine the impact of these amendments on plant growth the fast-growing Lupinus angustifolius L. a legume plant found close to the mining site, identified with the Regional Herbarium 'MARK' code: MARK-14861. This plant serves as a pivotal component in the comprehensive approach aimed at rehabilitating the soils surrounding the mining site. To identify the most effective organo-mineral amendment, we examined 65 combinations by mixing mine tailing soils with varying proportions (2.5%, 5%, 7.5%, or 10%) of the three distinct amendments and its combinations (Com), to identify the optimal rate for effective seeds germination and plant growth. Preliminary results on the germination index of L. angustifolius seeds revealed that four combinations (Com1: Cy2.5-Cp2.5-Mw10; Com2: Cy2.5-Cp5-Mw5; Com3: Cy7.5-Cp2.5-Mw7.5; and Com4: Cy10-Cp10-Mw10) maintained a high index due to improved soil properties. These combinations were used as the soil substrate material for a greenhouse experiment where plant growth, heavy metals accumulation, soil characteristics, and available metal content were determined. Our findings demonstrated that incorporating these four organo-mineral soil amendments into Kettara mine tailings led to a significant enhancement in plant growth. Notably, L. angustifolius plants exhibited a preferential accumulation of heavy metals in the root’s biomass (Cu: 565.60, Zn: 433.52, and Pb: 301.44 mg kg−1) with limited translocation to shoot parts (Cu: 37.44, Zn: 28.40, and Pb: 19.36 mg kg−1), particularly following the application of Com4. Furthermore, the properties of the mine soil were improved, including both neutralization of acidic soil pH (3 to ∼ 7) and reduction of metal elements bioavailability. Specifically, Com1, Com3, and Com4 effectively decreased the mobile fraction of metals in the tested mine tailings to exceptionally low levels achieving a reduction of 99% for copper, lead, and zinc, and 95% for arsenic. Our findings from this study indicated that our amendment holds promising potential for enhancing both plant growth and soil fertility, while simultaneously mitigating the mobility of heavy metals in heavy metal-contaminated acidic mine soils.

Rapid assessment of the main economic value indicators in lupine flour samples using infrared spectroscopy

Background. A calibration model has been developed for rapid assessment of economic value indicators (protein, oil, and quinolizidine alkaloid contents) in the seeds of narrowleaf lupine accessions from VIR using near-infrared spectroscopy, with the help of which it is possible to decide on the further use of the accessions.Materials and methods. Biochemical quality indicators (protein, oil, and quinolizidine alkaloid content) were studied in the seeds of narrowleaf lupine (Lupinus angustifolius L.) grown in 2019 in the northwest of Russia. Calibration models for measuring protein, oil and alkaloids in lupine seeds (62 accessions) were developed using a MATRIX-I IR analyzer (Bruker Optics, Germany). To construct calibration models, we used the values obtained by chemical analysis methods accepted at VIR. The oil content in lupine seeds was assessed by the defatted dry residue technique in Soxhlet extractors, protein by the Kjeldahl method, and quinolizidine alkaloids by gas chromatography coupled with mass spectrometry. All indicators were recalculated on the dry-weight basis.Results and conclusion. Statistical significance of the developed models was verified according to the results of measuring the content of protein, oil and alkaloids in the seeds of the test batch. The protein and oil content data obtained using a calibration curve did not differ significantly from the results of chemical studies, in contrast to alkaloid indicators. Consequently, the developed calibration model for the MATRIX-I IR analyzer can be used for rapid assessment of protein and oil content in narrowleaf lupine flour samples, thus accelerating the process of obtaining data on the main economic value indicators. The analysis does not require reagents and is safe.

Time maters: Exploring the dynamics of bioactive compounds content, bioaccessibility and antioxidant activity during Lupinus angustifolius germination

Germination is a process that enhances the content of health-promoting secondary metabolites. However, the bioaccessibility of these compounds depends on their stability and solubility throughout the gastrointestinal tract. The study aimed to explore how germination time influences the content and bioaccessibility of γ-aminobutyric acid and polyphenols and antioxidant capacity of lupin (Lupinus angustifolius L.) sprouts during simulated gastrointestinal digestion. Gamma-aminobutyric acid showed a decrease following gastrointestinal digestion (GID) whereas phenolic acids and flavonoids exhibited bioaccessibilities of up to 82.56 and 114.20%, respectively. Although the digestion process affected the profile of phenolic acids and flavonoids, certain isoflavonoids identified in 7-day sprouts (G7) showed resistance to GID. Germination not only favored antioxidant activity but also resulted in germinated samples exhibiting greater antioxidant properties than ungerminated counter parts after GID. Intestinal digests from G7 did not show cytotoxicity in RAW 264.7 macrophages, and notably, they showed an outstanding ability to inhibit the production of reactive oxygen species. This suggests potential benefit in mitigating oxidative stress. These findings contribute to understand the dynamic interplay between bioprocessing and digestion in modulating the bioaccessibility of bioactive compounds in lupin, thereby impacting health.

Proteomic analysis revealed that the oomyceticide phosphite exhibits multi-modal action in an oomycete pathosystem

Phytopathogenic oomycetes constitute some of the most devastating plant pathogens and cause significant crop and horticultural yield and economic losses. The phytopathogen Phytophthora cinnamomi causes dieback disease in native vegetation and several crops. The most commonly used chemical to control P. cinnamomi is the oomyceticide phosphite. Despite its widespread use, the mode of action of phosphite is not well understood and it is unclear whether it targets the pathogen, the host, or both. Resistance to phosphite is emerging in P. cinnamomi isolates and other oomycete phytopathogens. The mode of action of phosphite on phosphite-sensitive and resistant isolates of the pathogen and through a model host was investigated using label-free quantitative proteomics. In vitro treatment of sensitive P. cinnamomi isolates with phosphite hinders growth by interfering with metabolism, signalling and gene expression; traits that are not observed in the resistant isolate. When the model host Lupinus angustifolius was treated with phosphite, proteins associated with photosynthesis, carbon fixation and lipid metabolism in the host were enriched. Increased production of defence-related proteins was also observed in the plant. We hypothesise the multi-modal action of phosphite and present two models constructed using comparative proteomics that demonstrate mechanisms of pathogen and host responses to phosphite. SignificancePhytophthora cinnamomi is a significant phytopathogenic oomycete that causes root rot (dieback) in a number of horticultural crops and a vast range of native vegetation. Historically, areas infected with phosphite have been treated with the oomyceticide phosphite despite its unknown mode of action. Additionally, overuse of phosphite has driven the emergence of phosphite-resistant isolates of the pathogen. We conducted a comparative proteomic study of a sensitive and resistant isolate of P. cinnamomi in response to treatment with phosphite, and the response of a model host, Lupinus angustifolius, to phosphite and its implications on infection. The present study has allowed for a deeper understanding of the bimodal action of phosphite, suggested potential biochemical factors contributing to chemical resistance in P. cinnamomi, and unveiled possible drivers of phosphite-induced host plant immunity to the pathogen.

A perspective fungicide for protection of narrow-leaved lupin from diseases during the growth season

The article presents the laboratory and field tests results for the effectiveness of the fungicide Protazox SC (suspension concentrate) against anthracnose and other lupin diseases. The tests have been done in Bryansk region in 2020–2022. The study objects were seedlings and vegetative plants of the narrow-leaved lupin Vityaz variety. The effectiveness of protective and curative properties of the fungicide Protazox SC (azoxistrobine + protioconazole + dipheconazole, 200 + 125 + 60 g/l) was tested for 0.5; 1.0 and 1.5 l/ha consumption rates under laboratory conditions. The biological effectiveness was evaluated according to the number of infected seedlings grown in paper-and-plastic rolls compared to the control variant (fungicide free). The maximal rate of the fungicide – 1.5 l/ha – had the highest biological effectiveness of protective and curative properties (98.7 and 97.4 %); this corresponds to the effectiveness of the reference fungicide Kolosal Pro with the using rate of 0.4 l/ha. In a field test the fungicide Protazox at using rate of 1.5 l/ha was applied for lupin crops at the growing stages of “1-2 pairs of true leaves” and “bud formation” of lupin. The fungicide effectiveness was evaluated compared to the control without treatment. Biological effectiveness of the tested fungicide against anthracnose made 95.8 %, it was 4.5 % higher than of the reference fungicide Kolosal Pro. To the stage of “shiny pod” the number of anthracnose infected pods treated with the fungicide Protazox decreased to 1.5 % compared to 26.5 % in the control variant. Fusarium infection of plants decreased from 24.6 % in the control to 12.0 % in the variant with the fungicide. The spread of gray and white rot on beans decreased by 5.0 and 6.7 times, respectively. The viability of productive plants by the harvesting period increased by 41.3 %. Thanks to the application of the fungicide Protazox SC (1.5 l/ha) the significant narrow-leaved lupin seed yield rise of 0.91 t/ha was obtained (LSD05 = 0.04); the cost recovery was 2.56 rubles.