Lupinus Research Articles

Abscisic acid- and ethylene-induced abscission of yellow lupine flowers is mediated by jasmonates

The appropriate timing of organ abscission determines plant growth, development, reproductive success, and yield in relation to crop species. Among these, yellow lupine is an example of a crop species that loses many fully developed flowers, which limits the formation of pods with high-protein seeds and affects its economic value. Lupine flower abscission, similarly to the separation of other organs, depends on a complex regulatory network functioning in the cells of the abscission zone (AZ). In the present study, genetic, biochemical, and cellular methods were used to highlight the complexity of the interactions among strong hormonal stimulators of abscission, including abscisic acid (ABA), ethylene, and jasmonates (JAs) precisely in the AZ cells, with all results supporting that the JA-related pathway has an important role in the phytohormonal cross-talk leading to flower abscission in yellow lupine. Based on obtained results, we conclude that ABA and ET have positive influence on JAs biosynthesis and signaling pathway in time-dependent manner. Both phytohormones changes lipoxygenase (LOX) gene expression, affects LOX protein abundance, and JA accumulation in AZ cells. We have also shown that the signaling pathway of JA is highly sensitive to ABA and ET, given the accumulation of COI1 receptor and MYC2 transcription factor in response to these phytohormones. The results presented provide novel information about the JAs-dependent separation of organs and provide insight and details about the phytohormone-related mechanisms of lupine flower abscission.

Butterfly blues: population genetic assessment of wild lupine (Lupinus perennis L.) in endangered Karner blue butterfly habitat around central-west Michigan

Habitat degradation can have significant effects on native species inhabiting natural ecosystems. Within oak barrens and oak–pine barrens ecosystems, there is a complex interspecies interaction between the federally endangered Karner blue butterfly ( Lycaeides melissa samuelis) and its obligate host plant, wild lupine ( Lupinus perennis L.). Recruitment of wild lupine is critical for maintaining butterfly populations; however, this recruitment can be impeded by habitat fragmentation. Reduced recruitment can result in low genetic diversity in isolated populations, limiting its adaptive potential to respond to environmental change. This study was aimed at understanding the genetic diversity and population structure of wild lupine populations throughout central and west Michigan. We identified significant population structure across most of the populations sampled, with only two sites not significantly different from each other. No sites within our study area displayed statistically significant levels of inbreeding. There are also at least two genetic clusters of wild lupine present within our study region, although there is significant overlap among these groups, indicating that genetic differentiation among clusters may be limited.

Effect of lupin (Lupinus angustifolius) as a soybean meal replacement on the performance, meat quality, and blood parameters of broilers

This study investigated the effects of dietary lupin (LP) as a replacement for soybean meal (SBM) on the performance, meat quality, and blood parameters of broilers. A total of 960 1-day-old Ross 708 broilers were divided into four dietary groups. The four diets were formulated with different levels of dehulled LP content in place of SBM; LP0: 0, LP50: 50, LP100: 100, and LP200: 200 g/kg. There was a trend ( P < 0.10) for reduced weight gain in the LP200 group compared with other groups. The feed conversion ratio was higher ( P < 0.05) in the LP200 group than in the LP0 and LP50 groups. Concerning breast meat characteristics, the lightness color (L*) was lower ( P < 0.05) in the LP200-fed group compared with the LP0 group. Polyunsaturated fatty acids were higher ( P < 0.05) in LP100- and LP200-fed chickens than in LP0-fed chickens. Serum HDL cholesterol was significantly ( P < 0.05) higher in the group fed LP200 compared with the groups fed LP0 and LP50. A higher serum concentration of interleukin (IL)-2 was found in groups fed LP100 and LP200 than in groups fed LP0 and LP50. Our results suggest that LP could be a dose-dependent SBM substitute, and that the optimal level of LP inclusion is approximately 100 g/kg.

Lack of Evidence for Local Adaptation of the Endangered Karner Blue Butterfly to Its Sole Larval Hostplant—The Wild Lupine

Lack of Evidence for Local Adaptation of the Endangered Karner Blue Butterfly to Its Sole Larval Hostplant—The Wild Lupine

EPIP-Evoked Modifications of Redox, Lipid, and Pectin Homeostasis in the Abscission Zone of Lupine Flowers.

Yellow lupine is a great model for abscission-related research given that excessive flower abortion reduces its yield. It has been previously shown that the EPIP peptide, a fragment of LlIDL (INFLORESCENCE DEFICIENT IN ABSCISSION) amino-acid sequence, is a sufficient molecule to induce flower abortion, however, the question remains: What are the exact changes evoked by this peptide locally in abscission zone (AZ) cells? Therefore, we used EPIP peptide to monitor specific modifications accompanied by early steps of flower abscission directly in the AZ. EPIP stimulates the downstream elements of the pathway—HAESA and MITOGEN-ACTIVATED PROTEIN KINASE6 and induces cellular symptoms indicating AZ activation. The EPIP treatment disrupts redox homeostasis, involving the accumulation of H2O2 and upregulation of the enzymatic antioxidant system including superoxide dismutase, catalase, and ascorbate peroxidase. A weakening of the cell wall structure in response to EPIP is reflected by pectin demethylation, while a changing pattern of fatty acids and acyl lipids composition suggests a modification of lipid metabolism. Notably, the formation of a signaling molecule—phosphatidic acid is induced locally in EPIP-treated AZ. Collectively, all these changes indicate the switching of several metabolic and signaling pathways directly in the AZ in response to EPIP, which inevitably leads to flower abscission.

Effect of solid-state fermented and enzyme-supplemented lupins on performance and ileal amino acid digestibility in broiler chickens

Context The importance of lupin in animal nutrition has increased over the years due to its moderate protein content and relative availability. Low inclusion rate in broiler diet has been associated with the presence of antinutritional factors. The established beneficial effect of fermentation as a processing strategy and dietary enzyme use in improving the nutritional value of legumes such as lupins, thus, necessitated this study. Aims This study determined the effect of solid-state fermented lupin (SSFLP) and enzyme-supplemented lupin (LP) on the performance and ileal amino acid digestibility in broiler chickens. Methods In Experiment 1 (performance trial), a total of 300 day-old Ross 308 male broiler chicks were distributed into six dietary treatments, with five replicates of 10 birds each. Diet 1 was based on a corn–soybean meal (SBM), Diets 2 and 3 contained 250 g/kg LP and 250 g/kg SSFLP respectively. Diet 4 contained 250 g/kg LP + phytase (PHY). Diet 5 contained 250 g/kg LP + xylanase (XYL). Diet 6 contained 250 g/kg LP + PHY + XYL. PHY at 500 phytase units (FTU)/kg and XYL at 1000 units/kg were added to the respective diets. In Experiment 2 (digestibility trial), a total of 240 21-day-old Ross 308 male broiler chicks was assigned to six dietary treatments with five replicate cages of eight birds each. Diet 1 was based on SBM, Diets 2 and 3 contained 650 g/kg LP and 650 g/kg SSFLP respectively. Diet 4 contained 650 g/kg LP + PHY. Diet 5 contained 650 g/kg LP + XYL. Diet 6 contained 650 g/kg LP + PHY + XYL. Key results Dietary inclusion of SBM and LP + PHY enhanced broiler performance from 1 to 21 days. PHY- supplemented LP diet improved birds’ bodyweight gain and feed intake compared with the rest of the LP diets. Dietary inclusion of LP + PHY + XYL improved the apparent ileal digestibility (AID) of some amino acids (AA). SSF of LP had no effect on the AA contents, lowered AA digestibility and depressed weight gain in the birds. Although SSFLP diet did not improve performance, it effectively increased phosphorus (P) retention in broiler chickens. Feeding SSFLP and enzyme-supplemented LP diets enhanced the AID of calcium and P, as well as calcium retention. Conclusions Adding supplemental PHY to LP diet enhanced broiler performance, as indicated by the BWG and FI of these birds, which were significantly higher than those of birds fed on other LP-based diets and comparable with those of birds fed on the SBM diet from 1 to 21 days. Although the SSFLP diet did not improve broiler performance, it was effective in increasing P retention when compared with LP diets. The inclusion of LP + PHY + XYL diet improved the AID of amino acids. Implications SSFLP is a promising feed ingredient and can have a potential application in feed formulation. However, further studies are still needed to be able to clearly understand its effect at a high inclusion level on the performance and ileal amino acid digestibility in broiler chickens. The development of cocktail inoculants to target all ANFs in lupins will definitely open a new window for the poultry feed industry.

Restoration on Private Lands: A Case Study Examining Vegetation Recruitment following Restoration Treatments in an Oak-pine Barrens Ecosystem in Western Michigan, U.S.A.

Oak-pine barrens ecosystems provide critical habitat for the federally endangered Karner blue butterfly (Lycaeides melissa samuelis). Wild lupine (Lupinus perennis), an herbaceous perennial in this ecosystem is the only food source for the butterfly's larvae. The range and quality of these ecosystems have declined significantly. Restoration in private lands can be key to expanding these habitats, but often there is limited or no follow-up to assess the recovery of the site following restoration treatments. We conducted a case study in a private property in Newaygo County, Michigan for which prescribed fire had been implemented every 2 y from 2007 through 2011 to promote recruitment of key understory vegetation, but there had been no follow-up recovery assessment. The burn treatment consisted of a section that was not burned, one that was burned twice, and one that was burned three times. We collected data on vegetation groups and site related factors along this fire gradient to examine differences as a function of fire frequency, as well as delineate factors driving patterns of understory plant recruitment. Results show little tree recruitment from seedlings, but high densities of tree saplings recruited via resprouting. Estimated cover of lupine was low across all treatments, whereas Pennsylvania sedge (Carex pensylvanica Lam,) was highest among species in both cover and biomass. Lupine exhibited a negative relationship with Pennsylvania sedge, litter, and sprouted tree saplings, but a positive relationship with moss cover. We make recommendations for additional restoration interventions and highlight the need for continued support of private landowners as they engage in conservation of imperiled species.

Restoration on Private Lands: A Case Study Examining Vegetation Recruitment following Restoration Treatments in an Oak-pine Barrens Ecosystem in Western Michigan, U.S.A.

Oak-pine barrens ecosystems provide critical habitat for the federally endangered Karner blue butterfly (Lycaeides melissa samuelis). Wild lupine (Lupinus perennis), an herbaceous perennial in this ecosystem is the only food source for the butterfly's larvae. The range and quality of these ecosystems have declined significantly. Restoration in private lands can be key to expanding these habitats, but often there is limited or no follow-up to assess the recovery of the site following restoration treatments. We conducted a case study in a private property in Newaygo County, Michigan for which prescribed fire had been implemented every 2 y from 2007 through 2011 to promote recruitment of key understory vegetation, but there had been no follow-up recovery assessment. The burn treatment consisted of a section that was not burned, one that was burned twice, and one that was burned three times. We collected data on vegetation groups and site related factors along this fire gradient to examine differences as a function of fire frequency, as well as delineate factors driving patterns of understory plant recruitment. Results show little tree recruitment from seedlings, but high densities of tree saplings recruited via resprouting. Estimated cover of lupine was low across all treatments, whereas Pennsylvania sedge (Carex pensylvanica Lam,) was highest among species in both cover and biomass. Lupine exhibited a negative relationship with Pennsylvania sedge, litter, and sprouted tree saplings, but a positive relationship with moss cover. We make recommendations for additional restoration interventions and highlight the need for continued support of private landowners as they engage in conservation of imperiled species.

Genetic variation among white lupin (Lupinus albus L.) landraces from Northwestern and Southern Ethiopia for agronomic traits and nutrient contents of grain

White lupin (Lupinus albus L.) is rich in quality protein, relatively tolerant to drought, soil salinity and acidity, increase the fertility of soils and can contribute to improved agricultural sustainability, food security and reduce malnutrition which has close associations with climate change. This study was conducted to assess genetic variability of white lupin genotypes for agronomic traits and nutrient contents of grain, and to estimate association of traits. Twenty-five genotypes of white lupin were evaluated for 29 quantitative traits in 5 x 5 simple lattice designs at Holeta Agricultural Research Center during 2018/2019. The variations of genotypes for yield and grain protein content ranged from 122 to 3206 kg ha-1 and 28.55 and 35.81%, respectively. The genotypes had 2763, 772.3 and 81.59 mg/kg of average phosphorus, calcium and iron contents of grain. The PCV and GCV coefficient of variations varied from 4.39 to 29.54% and 3.41 to 28%, respectively. Heritability in broad sense and genetic advance as percent of mean ranged from 42.07 to 88.94% and 5.34 to 53.98%, respectively. The estimates of GCV, PCV, H2 and GAM were high to moderate. The research results showed the presence of variations among landraces of white lupin in Ethiopia and further evaluation of germplasm could be rewarding to improve the genetic resource in the country. Key words: Genetic advance, genotypic, phenotypic, heritability, protein and mineral content

Integrated Analysis of Small RNA, Transcriptome and Degradome Sequencing Provides New Insights into Floral Development and Abscission in Yellow Lupine (Lupinus luteus L.).

The floral development in an important legume crop yellow lupine (Lupinus luteus L., Taper cv.) is often affected by the abscission of flowers leading to significant economic losses. Small non-coding RNAs (sncRNAs), which have a proven effect on almost all developmental processes in other plants, might be of key players in a complex net of molecular interactions regulating flower development and abscission. This study represents the first comprehensive sncRNA identification and analysis of small RNA, transcriptome and degradome sequencing data in lupine flowers to elucidate their role in the regulation of lupine generative development. As shedding in lupine primarily concerns flowers formed at the upper part of the inflorescence, we analyzed samples from extreme parts of raceme separately and conducted an additional analysis of pedicels from abscising and non-abscising flowers where abscission zone forms. A total of 394 known and 28 novel miRNAs and 316 phased siRNAs were identified. In flowers at different stages of development 59 miRNAs displayed differential expression (DE) and 46 DE miRNAs were found while comparing the upper and lower flowers. Identified tasiR-ARFs were DE in developing flowers and were strongly expressed in flower pedicels. The DEmiR-targeted genes were preferentially enriched in the functional categories related to carbohydrate metabolism and plant hormone transduction pathways. This study not only contributes to the current understanding of how lupine flowers develop or undergo abscission but also holds potential for research aimed at crop improvement.

Relationships Among Population Size, Environmental Factors, and Reproduction in Lupinus perennis (Fabaceae)

Wild Lupine (Lupinus perennis) is an indicator of Midwestern U.S.A. oak savannas and the main larval food for endangered Karner Blue butterflies. In the past 100 y, this perennial has declined in distribution and abundance. To determine if this decline was related to population size and/or environmental factors and to better understand patterns of reproduction, we compared frequencies of seed coat colors, seed production variables, and environmental factors among 16 populations ranging in size from 25 to 8300 plants. Seedlings were germinated in the greenhouse to measure early offspring vigor. A seed color/substrate field experiment examined differences in seed removal to look at predation risk. Higher seed production was associated with increased population size, while fewer seeds per fruit were associated with increased bare ground, and higher fruits per stem and seed mass were found as soil pH increased. Although seedling emergence declined with increased bare ground, seedling growth was most correlated with pH and light, but not population size. Small populations had reduced seed color variation. Seed removal rates averaged 65% across all treatments, but dark-colored seeds had higher predation than light-colored seeds, regardless of substrate. Seed removal increased over time and was influenced by vegetative cover and distance to roads and water. Our results suggest managers should minimize the amount of bare ground and strive to maintain population sizes over 500 in order to restore and conserve Lupine populations.

Forage potential of three wild species of genus Lupinus (Leguminosae) from Mexico

The study of new legumes species in terms of yield and quality of forage under cultivation conditions is essential to improving the productivity of animal production. We evaluated the yield and nutritional quality of forage under irrigation conditions for three wild lupin species from Mexico (L. exaltatus, L. mexicanus, and L. rotundiflorus) at different stages of maturity. The experiment was conducted from November 2016 to March 2017 in Jalisco, Mexico. In general, the dry matter (DM) in all species increased with advancing maturity, but L. exaltatus presented the best production of forage 15, 143 kg/ha-1 DM, followed by L. mexicanus (9, 140 kg/ha-1 DM) on the third sampling date. The low forage yield in L. rotundiflorus was compensated by a higher average protein content (171.2 g/kg). The highest P and K content was recorded in L. rotundiflorus, whereas Ca and Mg were higher in the forage of L. mexicanus.

Biological soil crusts inhibit seed germination in a temperate pine barren ecosystem.

Biological soil crusts (BSCs) are known to affect plants’ germination and seedling establishment in arid ecosystems, but their ecological role in more mesic climates is not so well-known. We tested the effects of moss-crusted versus uncrusted soils on seed germination dynamics in a temperate pine barren ecosystem. We conducted a 35-day laboratory assay of seed germination on moss-crusted soils versus uncrusted soils from the Albany (NY) Pine Bush Preserve. We compared total seed germination and the number of days to 50% of total germination of two herbaceous perennial forb species in each soil type. Three and five times more seeds germinated on uncrusted soil than on crusted soil for bush clover (Lespedeza capitata) and wild lupine (Lupinus perennis), respectively. Seeds of both species also germinated approximately 10 days earlier on uncrusted soil than on crusted soil. This study, and others in similar habitats, show that BSCs in mesic climates can influence germination and other early life-history stages of plants. We hope that further study of the interactions between BSCs and vascular plants in mesic climates will contribute to our understanding of the ecology of BSCs outside the arid and semiarid climates where they are more extensively studied.

Seedling emergence ofLupinus diffususin response to abrasion in an electric seed scarifier

Seedling emergence of<i>Lupinus diffusus</i>in response to abrasion in an electric seed scarifier

Detection and quantification of black foot and crown and root rot pathogens in grapevine nursery soils in the Western Cape of South Africa

Black foot disease (BFD) and crown and root rot (CRR) are important soilborne diseases that affect young grapevines in nurseries and vineyards. A 3-year survey (2013–2015) of five open-field grapevine nurseries was conducted in the Western Cape Province of South Africa. The survey involved the isolation of BFD and CRR pathogens from grafted rootstocks (ten plants per nursery, per year) that were rooted in soil for 1 year. In 2013 and 2015, grapevines were sampled, while in 2014, sampling was focused on rotation crops and weeds (ten plants each). The rotation crops included white mustard, lupins, canola, triticale and forage radish. The weed species sampled included Johnson grass, ryegrass, winter grass, Cape marigold and corn spurry. Soil samples from ten sites per nursery were also collected in close proximity to the sampled plants, at depths of 0–30 cm and 30–60 cm (ten samples per depth). Isolations were made from the grapevines, rotation crops and weeds. Pathogen detection and quantification in the soil were determined using quantitative real-time polymerase chain reaction technology. The predominant BFD pathogens isolated from grapevines were Campylocarpon fasciculare , Ca. pseudofasciculare and Dactylonectria macrodidyma . The predominant CRR pathogens were Pythium irregulare and Phytopythium vexans. Dactylonectria macrodidyma , D. novozelandica , D. pauciseptata , Py. irregulare , Py. ultimum var. ultimum and Py. heterothallicum were isolated from triticale roots. Dactylonectria spp. were also isolated from corn spurry, while Py. irregulare and Py. ultimum var. ultimum were isolated from numerous weeds and rotation crops. Mean soil DNA concentrations of Ilyonectria and Dactylonectria were from 0.04 to 37.14 pg μL -1 , and for Py. irregulare were between 0.01 and 3.77 pg μL -1 . The Phytophthora mean soil DNA concentrations ranged from 0.01 to 33.48 pg μL -1 . The qPCR protocols successfully detected and quantified BFD and CRR pathogens in grapevine nursery soil. This is the first report of D. pauciseptata and D. alcacerensis in South African grapevine nurseries.

Effects of Physalis peruviana and Lupinus albus on malondialdehyde, glutathione, cholesterol, vitamins and fatty acid levels in kidney and liver tissues of diabetic rats

In this study, the effects of goldenberry and lupin on lipid peroxidation and antioxidant system parameters of liver and kidney tissues of streptozotocin-induced diabetic rats were investigated. Type II diabetes was produced in rats by the streptozotocin intraperitoneal injection. Albino rats were divided into four groups, each one containing 10 rats: non-diabetic control group, STZ-Diabetes type II group, STZ-Diabetes+goldenberry type II group, and STZ-Diabetes+lupin type II group. After one week from the injection, goldenberry and lupin were intraperitoneal injected to rats for 2 months. Malondialdehyde, glutathione, cholesterol, and fatty acid levels, which are signs of lipid peroxidation, were measured in these tissues. At the beginning and end of the study, postprandial blood glucose levels and weights of the rats were measured. In type II diabetes, malondialdehyde increased compared to the control group. Glutathione decreased in the other tissues and all of the streptozotocin-induced diabetic groups. In type II diabetes, liver cholesterol levels increased. Treatment with similar doses of goldenberry and lupin extracts significantly reduced postprandial hyperglycemia, oxidative stress, and augmented antioxidant system. The results of the present study showed that the herb suspensions exerted anti-hyperglycemic effects and consequently may alleviative liver and kidney damage caused by streptozotocin-induced diabetes.

Influences of Physalis peruviana L. and Lupinus albus L. fruits extracts on the levels of some biochemical parameters in brain and muscle tissues of type II diabetic rats

New functional foods are increasingly sought to improve the treatment of diseases related to glucose and lipid metabolism. The present study was designed to investigate the possible antidiabetic and antioxidant effects of goldenberry and lupin on brain and muscle tissues of streptozotocin-induced type II diabetic rats. Type II diabetes was produced in albino rats by the streptozotocin injection. Wistar albino rats were divided into four groups, each one containing 10 rats: non-diabetic control group, STZ- type II Diabetes group, STZ- type II Diabetes+goldenberry group, and STZ- type II Diabetes+lupin group. After one week from the injection, goldenberry and lupin were injected to rats for 2 months. Malondialdehyde, glutathione, cholesterol, and fatty acid levels, which are signs of lipid peroxidation, were measured in brain and muscle tissues. In type II diabetes, malondialdehyde increased compared to the control group. Glutathione decreased in the both tissues and all of the streptozotocin-induced diabetic groups. In type II diabetes, brain cholesterol levels decreased. Treatment with similar doses of goldenberry and lupin significantly reduced oxidative stress, augments antioxidant system and altered fatty acid metabolism in these tissues, thereby maintaining favourable fatty acid distribution affected by diabetic complications. These results validate the use of goldenberry and lupin fruits as a treatment against diabetes mellitus and its complications and suggest it is suitable to continue studies for its safe therapeutic use.

Use of sweet lupin, canola oilcake and full-fat canola as alternatives to soybean oilcake in diets for broilers

CITATION: Brand, T. S., et al. 2018. Use of sweet lupin, canola oilcake and full-fat canola as alternatives to soybean oilcake in diets for broilers. South African Journal of Animal Science, 48(3):553-562, doi:10.4314/sajas.v48i3.16.

The influence of dietary inclusion of peas, faba bean and lupin as a replacement for soybean meal on pig performance and carcass traits

The influence of dietary inclusion of peas, faba bean and lupin as a replacement for soybean meal on pig performance and carcass traits

The effect of addition of yellow lupin seeds (&lt;i&gt;Lupinus luteus&lt;/i&gt; L.) to laying hen diets on performance and egg quality parameters

The effect of addition of yellow lupin seeds (&lt;i&gt;Lupinus luteus&lt;/i&gt; L.) to laying hen diets on performance and egg quality parameters