Lungs Of Wistar Rats Research Articles

Effects of aqueous seed extract of <i>Garcinia kola</i> on spray paint-induced histological changes in the lungs of Wistar rats

The most common cause of lung diseases among people with spray paint-related occupations is hydrocarbon poisoning which has significant morbidity and mortality if left untreated. This study aimed to investigate the effects of aqueous seed extract of Garcinia kola on spray paint-induced interstitial pneumonitis in the adult Wistar rats. Thirty adult Wistar rats weighing between 240 g and 270 g were divided into five groups of six rats each. Animals in group A were placed on feed and water only. Animals in group B were exposed to 100 ml of spray paintfumes via inhalation. Animals in group C received 500mg/kg body weight per day of Garcinia kola. Animals in group D were exposed to 100 ml of spray paint fumes via inhalation and received 250mg/kg body weight per day of Garcinia kola. Animals in group E were exposed to 100 ml of spray paint fumes via inhalation and received 500mg/kg body weight per day of Garcinia kola. The extract was administered for 30 consecutive days via an orogastric tube. Groups A, C, D and E revealed normal histoarchitecture of the lung; normal alveoli and normal interstitial space. There were observable histological variations in the lung tissues of Group B rats which include severe alveolar dilation, interstitial haemorrhage, interstitial infiltrates of inflammatory cells, severe vascular ulceration and congestion (evidence of interstitial pneumonitis). It is concluded that Garcinia kolahasan ameliorative effect on spray paint-induced histological changes in the lungs of Wistar rats.

Effects of Bryophyllum pinnatum on Dysfunctional Autophagy in Rats Lungs Exposed to Zinc Oxide Nanoparticles

Lung inflammation as a result of exposure to toxicants is a major pathological problem. Autophagy (AP) is a process of cell self-digestion and can be disrupted by environmental toxicants, leading to oxidative stress, inflammation and cellular damage. Bryophyllum pinnatum (Lam.) Oken has been used in folklore medicine to manage pathological abnormalities, including inflammation, but mechanisms remain unclear. This work investigated the effects of Bryophyllum pinnatum ethanol leaf extract (BP) on dysfunctional AP in the lungs of Wistar rats exposed to zinc oxide nanoparticles (ZONPs). The experimental rats were orally administered ZONPs for seven days (10 mg/kg). Some exposed rats were post-treated with BP (62.5 and 125 mg/kg) through oral gavage. Oxidative stress, inflammation, and apoptotic and autophagic parameters were assessed using biochemical assay and gene expression methods. Several indices of pulmonary damage were also evaluated. PCR analysis suggested that ZONP downregulated the expression of pro-autophagy-related genes (Beclin 2, ATG5, DAPK, and FOXP3) and upregulated the expression of the TNF-alpha, NF-Kb, LC3 and Bcl2 genes. In contrast, BP significantly (p < 0.0001) reversed ZONP-induced pulmonary toxicity and oxidative stress. It reduced MDA levels and increased SOD, CAT, GSH and GPxD activities. BP significantly (p < 0.0001) downregulated the expressions of proinflammatory genes (IL-6 and JNK) and upregulated the expressions of IL-10, CAT and SOD genes in ZONP-exposed rats. BP restored the lung’s histoarchitectural structure after ZNOP-induced distortion. The results suggested that BP has antioxidant and anti-inflammatory properties, and could effectively restore ZNOP-induced dysfunctional AP in the lungs of Wistar rats.

Multimodal pulmonary clearance kinetics of carbon black nanoparticles deposited in the lungs of rats: the role of alveolar macrophages

BackgroundAlveolar macrophages (AMs) have been predicted to affect the pulmonary clearance of nanomaterials; however, their qualitative and quantitative roles are poorly understood. In this study, carbon black nanoparticles (CBNPs) were instilled into the lungs of Wistar rats at 30, 100, and 300 µg/rat. The concentrations of particles in organs, including the lung, lung-associated lymph nodes (LALN), liver, spleen, and kidney, were evaluated at days 0 (immediately after instillation), 1, 7, 28, 60, and 90 post-instillation.ResultsThe results indicated a multimodal pulmonary clearance pattern for CBNPs: slow clearance until day 28, fast clearance from days 28 to 60, and slow clearance from days 60 to 90. To determine the mechanism of this unique clearance pattern, CBNPs were instilled into AM-depleted rats using clodronate liposomes (CLO). At 28 days after instillation, the CBNP levels in the lungs treated with CLO showed about 31% higher reduction than in normal rats. In addition, the concentration of CBNPs in LALN treated with CLO significantly increased on day 28, whereas in normal rats, no detectable levels were observed.ConclusionsThis result highlights that the prolonged retention of poorly soluble NPs in the lung until day 28 is mediated by the phagocytosis of AMs, and the fast clearance between days 28–60 is due to the turnover time of AMs, estimated around 1–2 months after birth. Similarly, new generations of AMs mediate the slow phase between days 60 and 90. However, further studies are needed to understand the multimodal clearance mechanism and the modulation of pulmonary clearance of poorly soluble NPs.

Cysteamine Nanoemulsion Delivery by Inhalation to Attenuate Adverse Effects of Exposure to Cigarette Smoke: A Metabolomics Study in Wistar Rats.

There is a pressing need for novel pharmacological interventions and drug delivery innovations to attenuate the cigarette smoke-associated oxidative stress and lung disease. We report here on the attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR) and metabolomics of Wistar rats exposed to cigarette smoke for 28 days. The animals were treated for 15 days with plain cysteamine given orally or cysteamine as nanoemulsion given orally or via inhalation. The study design also included two control groups as follows: rats exposed to cigarette smoke but did not receive a treatment (diseased control group) and rats neither exposed to cigarette smoke nor a treatment (normal control group). The targeted metabolomics using Parallel Reaction Monitoring showed that in the diseased control group, ornithine, nicotinamide, xanthine, hypoxanthine, and caprolactam were increased compared with the normal control group. In addition, (±)8(9)-DiHET, which was initially downregulated in the diseased control group, exhibited a reversal of this trend with cysteamine nanoemulsion given via inhalation. The cysteamine nanoemulsion delivered by inhalation highlighted the importance of the route of drug administration for targeting the lungs. To the best of our knowledge, this is the first work to use ATR-FTIR and metabolomics in Wistar rat lung tissues, suggesting how cysteamine nanoemulsion can potentially reduce cigarette smoke-induced oxidative damage. The metabolites reported herein have potential implications for discovery of novel theranostics and, thus, to cultivate diagnostic and therapeutic innovation for early prevention and treatment of cigarette smoke-associated lung diseases.

Toxicological evaluation of copper oxide nanoparticles following their intraperitoneal injection to Wistar rats.

Copper oxide (Cu2O) nanoparticles (CO NPs) are in extensive use during our everyday life as antimicrobial agent, lubricant, in manufacturing electrodes of lithium ion batteries as well as for photo catalytic degradation of organic pollutants. Due to extensive and diverse use Cu2O NPs, they are likely to accumulate in the environment and to affect the live forms. Present investigation was aimed to report the biocompatibility of CO NPs in Wistar rats in sex specific manner. CO NPs, having average diameter of 14.06nm, were synthesized by co-precipitation method and scanning electron microscopy and X ray diffraction were used for their characterization. For 14 consecutive days, Wistar rats (6weeks old) of both sexes were intraperitoneally injected with 10mg/mL saline/Kg body weight of CO NPs, while the control groups intraperitoneally received saline solution for same duration. Behavioral tests (open field and novel object recognition), complete blood count, selected biomarkers of oxidative stress and Copper concentration in brain and liver were determined in all subjects. High mortality rates [male 40% and female 60%] were observed in rats exposed to CO NPs. A sever decrease in body weight was also observed in both male and female rats exposed to CO NPs. Female rats treated with CO NPs spent significantly more time with novel object as compared to control [P=0.05] during second trial of novel object test. CO NPs treated female rats had higher mean corpuscular hemoglobin [P<0.001] levels and Copper concentration in liver [P=0.04] than control. Male rats exposed to CO NPs had significantly higher mean corpuscular volume [P=0.02] and superoxide dismutase [SOD] [P=0.04] in lungs than their control group. All other studied parameters non significantly varied upon comparison between CO NPs treated and untreated rats of both sex. In conclusion, we are reporting that intraperitoneal injections of CO NPs for 14days can disturb complete blood count and biomarkers of oxidative stress in lungs of Wistar rats.

Genotoxic Response and Histological Alterations in Rat Lungs Exposed to Gasoline Generator Exhaust

The epileptic power supply in Nigeria has caused a surge in the production of energy from other sources, including gasoline generator sets. The air pollution caused by the exhaust from these engines has been implicated in a variety of metabolic disorders and diseases, including cancer. This study aims at understanding the genotoxic and histopathological effects of gasoline generator exhaust exposure on the DNA and lungs of adult male wistar rats. Forty- eight (48) adult wistar rats were divided into four categories, each with 12 rats. The control rats were not exposed to gasoline generator exhaust while the test groups were exposed at 2, 4, and 8-hour intervals for a duration of 4, 8, and, 12 weeks respectively. Animals were euthanized using cervical dislocation and blood samples were obtained for genotoxic analysis via cardiac puncture while the lungs were preserved for 72 hours in 10% neutral buffered formalin and further processed for histological studies. The Olive Tail Moment (OTM) of the exposed rats showed significant variation across the exposure time points when compared to the unexposed control, indicating that exposure to gasoline generator exhaust negatively impacts the DNA. Additionally, histological investigations also indicated some cytopathic characteristics, such as peribronchiolar and perivascular infiltration by inflammatory cells, congestion, and thickening of blood vessels. The findings of this study showed that exposure to gasoline generator exhaust induces genotoxic damage with the severity of the damage occurring in a time/exposure-dependent manner. Keywords: Air pollution; Nigeria; Gasoline; Generator; Genotoxicity.

The Effect of Moringa Leaf Extract (moringa oleifera) on the Microscopic Structure of the Lungs of Male Wistar Rats Given Inhalation of E-cigarette Liquid Vapor

Background: Moringa leaf extract is proven to have high antioxidant bioactive compounds and acts as an anti-inflammatory and prevents cell damage due to exposure to free radicals or oxidative. In the respiratory system exposed to Electronic Delivery Nicotine System liquid vapor, an inflammatory response and oxidative stress occur. Objective: This study aimed to determine the effect of Moringa leaf extract (Moringa oleifera) on the microscopic structure of the lungs of male Wistar rats given inhalation of e-cigarette liquid vapor. Methods: This research is an experimental laboratory with Post Test Only Control Group Design. Rats were divided into 4 groups containing 6 rats in each group, determined based on simple random sampling with the following divisions: Aquadest 3 mL (K1), gave inhalation of liquid vapours of electric cigarettes (K2), gave inhalation of liquid vapours of electric cigarettes + Moringa leaf extract with the dekoxy method dose of 500 mg/kgBW (P1), administration of vapor inhalation of e-cigarette liquid + Moringa leaf extract with the dekoxy method at a dose of 500 mg/kgBW (P2). Termination of the experimental animals was carried out on the 15th day and the rat pulmonary organs were taken to assess the microscopic structure of the rat lung. Results: The results of the Kruskal-Wallis test and continued with the Mann-Whitney test showed a significant difference (p &lt; 0.05), however, there was no significant difference in the degree of pulmonary damage in the P1 to P2 groups. Conclusion: There is an effect of Moringa leaf extract (Moringa oleifera) on the microscopic structure of the lungs of male Wistar rats given inhalation of liquid vapor of electric cigarettes.

Multimodal Diagnostics of Changes in Rat Lungs after Vaping.

(1) Background: The use of electronic cigarettes has become widespread in recent years. The use of e-cigarettes leads to milder pathological conditions compared to traditional cigarette smoking. Nevertheless, e-liquid vaping can cause morphological changes in lung tissue, which affects and impairs gas exchange. This work studied the changes in morphological and optical properties of lung tissue under the action of an e-liquid aerosol. To do this, we implemented the "passive smoking" model and created the specified concentration of aerosol of the glycerol/propylene glycol mixture in the chamber with the animal. (2) Methods: In ex vivo studies, the lungs of Wistar rats are placed in the e-liquid for 1 h. For in vivo studies, Wistar rats were exposed to the e-liquid vapor in an aerosol administration chamber. After that, lung tissue samples were examined ex vivo using optical coherence tomography (OCT) and spectrometry with an integrating sphere. Absorption and reduced scattering coefficients were estimated for the control and experimental groups. Histological sections were made according to the standard protocol, followed by hematoxylin and eosin staining. (3) Results: Exposure to e-liquid in ex vivo and aerosol in in vivo studies was found to result in the optical clearing of lung tissue. Histological examination of the lung samples showed areas of emphysematous expansion of the alveoli, thickening of the alveolar septa, and the phenomenon of plasma permeation, which is less pronounced in in vivo studies than for the exposure of e-liquid ex vivo. E-liquid aerosol application allows for an increased resolution and improved imaging of lung tissues using OCT. Spectral studies showed significant differences between the control group and the ex vivo group in the spectral range of water absorption. It can be associated with dehydration of lung tissue owing to the hyperosmotic properties of glycerol and propylene glycol, which are the main components of e-liquids. (4) Conclusions: A decrease in the volume of air in lung tissue and higher packing of its structure under e-liquid vaping causes a better contrast of OCT images compared to intact lung tissue.

In Silico Assessment of Naphthalene Interaction with Glutathione Reductase and Histological Changes in Naphthalene Exposed Rats Tissues

Naphthalene (NA), a bicyclic aromatic compound that is widely used in various commercial and industrial applications including lavatory scent disks and mothballs, is known to be readily absorbed into the systemic circulation following either inhalation or ingestion and may cause systemic toxicity. This study examined the histopathological changes in some tissues (lung, liver and kidney) of NA-exposed Wistar rats. Twenty-four rats (175-250 g) were randomly divided into six groups of four rats each. The rats in all groups were given food and water while in addition rats in the experimental groups were exposed to NA at 0.75 mg/m3 and 1.50 mg/m3 for 2 h and 4 h. Rats were sacrificed at 24 h after the last hour of NA exposure. The tissues were excised for histological examination. The effects of NA and some selected metabolites using the rat glutathione reductase homology model were examined via molecular docking and dynamic simulation. From this study, exposure of rats to NA resulted in hepatocyte necrosis at 0.75 mg/m3 for 2 h, dilation of the alveolar duct of the lung at 0.75 mg/m3 and severe epithelial hyperplasia and chronic infiltration of inflammatory cells in the lung at 1.50 mg/m3 for 4 h. Also, vascular congestion was observed in the kidneys at 0.75 mg/m3 for 2 h and 1.50 mg/m3 for 4 h. The in silico study revealed the NA metabolite toxic potential, and NA showed the lowest binding score (-5.40± 0.00 kcal/mol). Therefore, the study concludes that NA exposure irrespective of duration can lead to hepatic and alveolar damage in the absence of mechanisms that can ameliorate its toxic effects.

Molecular Imaging of Radiation-Induced Lung Injury Using [18F]AlF Labeled Polypeptide Targeting C-X-C-Chemokine-Receptor-Type-4

Radiation-induced lung injury (RILI) is a severe side effect of radiotherapy (RT) for thoracic malignancies and we currently lack established means for the early detection of RILI. In this study, we synthesized a new tracer, [18F]AlF-NOTA-QHY-04, targeting C-X-C-chemokine-receptor-type-4 (CXCR4) and investigated the feasibility of using this tracer in positron emission tomography combined with computed tomography (PET/CT) for detecting RILI. An RILI model was established using a high-dose single RT (50 Gy/Fraction) to the right lung of female Wistar rats. Experimental animals were scanned with [18F]AlF-NOTA-QHY-04 PET/CT and [18F]FDG PET/CT at various time-points post-RT. MicroCT imaging was conducted weekly till 11 weeks post-RT. Dynamic, competition, autoradiography and histopathological studies were performed on day 14 post-RT. Biodistribution study was further performed on day 18 post-RT. Lung QHY-04 uptake was analyzed in twelve patients with radiation pneumonia, developed during or after thoracic RT. The yield of [18F]AlF-NOTA-QHY-04 was 28.5-43.2%, and the specific activity was 27-33 GBq/μmol. Significantly increased [18F]AlF-NOTA-QHY-04 uptake in the damaged lung compared with that in the normal lung was observed in the experimental animal model on 6 post-RT and peaked on day 14 post-RT (0.96 ± 0.06 vs. 0.50 ± 0.05 %ID/mL, P<0.05), whereas no apparent uptake of [18F]FDG was shown on day 7 and 15 post-RT. Significant intense [18F]AlF-NOTA-QHY-04 uptake was confirmed by autoradiography. No significant difference in CT density was observed between the damaged and normal lung tissues until six weeks post-RT. Immunofluorescence staining demonstrated expression of CXCR4 was significantly increased in the damaged lung tissue, which correlated with results obtained from hematoxylin-eosin and Masson's trichrome staining. Maximum standardized uptake values (SUVmax) were significantly higher in the irradiated lung compared with that in the normal lung in 12 patients with radiation pneumonia (3.07 ± 0.86 vs. 0.585 ± 0.17, P<0.001). SUVmax of patients with grade 2 RILI was significantly higher than that of patients with grade 1 RILI (3.30 ± 0.65 vs. 1.64 ± 0.08, P<0.05). This study indicated that [18F]AlF-NOTA-QHY-04 PET/CT imaging can detect RILI noninvasively and earlier than [18F]FDG PET/CT in a rat model. Clinical studies verified its' feasibility, suggesting the clinical potential of [18F]AlF-NOTA-QHY-04 as a PET/CT tracer for early monitoring of RILI.

Exercise intensities modulate ACE2/MasR/eNOS pathway in male Wistar rat's lung.

Specific exercise intensities could improve lung vascular function by increasing nitric oxide (NO). The ACE2/MasR/eNOS axis is one of the pathways facilitating NO synthesis. This study examines the effect of different intensities of aerobic training on the ACE2/MasR/eNOS axis and histology of lung muscular arteries. Male Wistar rats were used in this study and randomized into control and exercise groups receiving low-, moderate-, and high-intensity training. The training was conducted for 30 min daily, five times a week, for 8 weeks. We observed that different exercise intensities affect the ACE2/MasR/eNOS pathway differently. Compared to control, high-intensity aerobic exercise significantly increased ACE2, Mas receptor (MasR), and eNOS mRNA expressions (p < 0.01). Moderate-intensity exercise significantly increased MasR and eNOS mRNA expressions compared to the control (p < 0.05), and this intensity also increased ACE2 mRNA but not significantly. Low-intensity exercise increased ACE2, MasR, and eNOS mRNA expressions but not significantly. Low-, moderate-, or high-intensity exercises reduced the medial wall thickness of the lung muscular arteries but not significantly. In conclusion, high-intensity exercise may induce NO synthesis in the lung by increasing mRNA expression of ACE2, MasR, and eNOS without decreasing the medial wall thickness of the muscular artery. Thus, high-intensity exercise may be the optimal intensity to improve NO synthesis and vascular function in the lung.

Effects of aqueous leaf extract of Mimosa pudica on insect repellent-induced injury in the lungs of adult Wistar rats

The aim of this study was to investigate the effects of aqueous leaf extract of Mimosa pudica in the lungs of adult Wistar rats exposed to insect-repellent mist. 30 adult Wistar rats were randomly assigned into 5 groups; group A-E comprising of 6 rats per group. Group A rats were placed on rat food and water only. Group B rats were exposed to insect repellant only via inhalation. Group C rats were given Mimosa pudica extract orally at 500mg/Kg body weight per day (BWT/D) and Group D rats were exposed to Mosquito repellent and received low dose of the plant extract at (250mg/kg BWT/D) and Group E rats were exposed to mosquito repellent and received (500mg/kg BWT/D) of extract. The dosages were given for 30 consecutive days via orogastric method. There was significant increase in body weight in all treated groups. The haematological outcome showed that insect repellent caused some derangements in haematological parameters especially haematocrit, plateletcrit and mean corpuscular hemoglobin concentration. Histologically, Group D showed an expanded lumen of the bronchioles and shrunken activated immune system. Group E showed a more activated immune system and contracted bronchial artery. Mosquito repellant induced vascular ulceration, bronchiolar mucosal ulceration and activated lymphoid tissue in Group B. These effects were reversed by Mimosa pudica leaf extract. In conclusion, Mimosa pudica had ameliorative effect against mosquito repellant induced injury in the lungs.

Effects of Aqueous Leaf Extract of Ageratum conyzoides on Spray-Paint-Induced Injury in the Lungs of Adult Wistar Rats

The aim of this study was to investigate the effects of aqueous leaf extract of Ageratum conyzoides on spray-paint-induced injury in the lungs of adult Wistar rats. The 30 adult Wistar rats weighing between 250g and 280g that were used in this research were divided into 5 groups of six 6 rats per group. The haematological outcome showed that spray paint caused some derangements in haematolodical parameters especially lymphocytes, monocytes, granulocytes and platelets. Histologically, interstitial haemorrhage, pulmonary edema, and activation of lymphoid tissue were observed in the rat treated with spray paint only. Ageratum conyzoides shrunk the local activated immune system and the florid-activated broncho-alveolar aggregates of the immune system in the Ageratum conyzoides treated rats. In conclusion, Ageratum conyzoides has ameliorative effects against spray-paint-induced injury in the lungs of Wistar rats and its effect is inversely proportional to dosage. It is more potent at low doses.

Pengaruh Pemberian Kombinasi Ekstrak Kulit Pisang dan Kulit Nanas terhadap Indeks Organ Tikus Wistar

Banana peel and pineapple peel contain potassium which may be developed into health supplements. The development of banana peels and pineapple peels as ingredients for potassium supplements must meet safety aspects that can be proven by subchronic toxicity tests. This subchronic toxicity test aimed to determine the effect of repeated administration of a combination of banana peel and pineapple peel extract for 28 days on the liver, kidney, kidney, lung, heart, and spleen index of Wistar rats. This research used male and female white rats which were divided into six treatment groups, namely the control group, low dose, middle dose, high dose, satellite control, and high dose satellite. The results showed that there was a significant increase in the liver and kidney organ index, and a significant decrease in the adrenal gland organ index after administration of a combination of banana peel and pineapple peel extract. This research concludes that the combination of banana peel and pineapple peel extract has an effect on the liver, kidney, and adrenal gland organ index in Wistar white rats.

Antioxidant effect of ethanolic extract of Pleurotus Ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH) level in lung rats exposed to cigarette smoke

Background: Cigarette smoke can trigger oxidative stress. An alternative to overcome the harmful effects of cigarette smoke is through antioxidants. White oyster mushrooms (Pleurotus Ostreatusostreatus or P. ostreatus) are a source of exogenous antioxidants because many contain active compounds for potential antioxidants such as phenol and flavonoid compounds. The purpose of this study was to analyze the effect of ethanolic extract of Pleurotus P.ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH), as well as to analyze their correlation in the lung of Wistar male rats exposed to cigarette smoke. Methods: The study was a preclinical experiment conducted on 24 rats divided into four groups. The treatment was carried out for 42 days and antioxidant effects were assessed through levels of HNE and GSH in rat lungs. Groups were divided as follows: I- normal control, II- negative control, III and IV exposed to cigarette smoke for 60 minutes/day. Group III (treatment group) was treated with ethanolic extract of PleurotusP. ostreatus at 250 mg/kg BW rat/day, and group IV (comparison group) was treated with N-acetyl cysteine 600 mg /day. Data analysis used was one-way ANOVA and Kruskal Wallis test, and Spearmen rank correlation coefficient test. Results: The results showed that the group receiving ethanolic extract of PleurotusP. ostreatus had HNE levels of (44,18 ± 2,09 pg/mL) and GSH (0,04 ± 0,00 pg/mL) protein. This extract significantly increased GSH levels and inhibited the increase of HNE levels. Results of GSH (p≤0.01) showed significant results using one-way ANOVA. Conclusions: The ethanolic extract of P. ostreatus can prevent lipid peroxidation and decrease endogenous antioxidant levels in lung cells exposed to cigarette smoke. Ethanolic extract of PleurotusP. ostreatus has good antioxidant potential.

Micro anatomical effects of aqueous cactus extract in the lungs of a diabetic Wistar rat

Opuntia stricta is a naturally occurring plant and is readily available in some parts of Zambia where it is used for its decorative nature and its fruits consumed. This present laboratory based study was carried out to assess some micro anatomical effects of aqueous cactus on the lungs of a diabetic Wistar rat. The male adult Wistar rats (30) weighing between 160-200 g were indiscriminately classified into five groups consisting of 6 Wistar rats namely: control, diabetic control, Diabetes+ Metformin, Diabetes+ cactus and cactus only groups. Diabetes was induced with Streptozotocin dose 70 mg/kg BW and sustained increase in blood glucose levels after 72 hours and treatment commenced with aqueous cactus extract and Metformin with 100 mg/kg body weight respectively and was administered orally for 4 weeks . Blood glucose levels (mmol/L) were monitored. After 4 weeks of treatment the animals were sacrificed by euthanasia, the lungs were excised, weighed and fixed in 10% formal saline. Data was analyzed using Excel and one way ANOVA. p&lt;0.05 was considered significant. Change in body weight and relative organ weight of the Diabetic+ cactus and Diabetes + Metformin was significant when compared to diabetic group(p&lt;0.05). Diabetic + cactus became normoglycemic at week 3 and diabetic+ Metformin at week 4. The histological examination of the lungs of the diabetic rats showed disruption in cytoarchitecture with necrotic pneumocyte. Diabetic + cactus and Diabetes + Metformin showed no disruption in their cytoarchitecture and both had healthy pneumocyte present. Cactus only was similar to control. In conclusion, aqueous extract of Cactus showed ameliorative effects on the lung of adult male Wistar rats against the damage caused by diabetes.

Assessment of different formalin concentrations on the histochemical staining of liver, kidneys and lungs of Wistar rats

Assessment of different formalin concentrations on the histochemical staining of liver, kidneys and lungs of Wistar rats

Pengaruh Pemberian Dehydrepiandrosterone (DHEA) Terhadap Paru-Paru Tikus Wistar Jantan Yang Terpapar Asap Rokok

This study investigated the effect of dehydroepiandrosterone on the lungs of male Wistar rats exposed to cigarette smoke. A total of 21 male Wistar rats were divided into 3 groups, namely the first group (negative control exposed to cigarette smoke for 14 days), the second group (given DHEA at a dose of 15 mg/kgBW and exposure to cigarette smoke), the third group (given DHEA at a dose of 30 mg/kgBW and exposure to cigarette smoke). DHEA was administered orally through a cannula 30 minutes before exposure to cigarette smoke with an exposure duration of about 25-40 minutes, equivalent to 4 cigarettes/day. After 14 days of experimentation, the rats were euthanized. Right lungs were harvested for malondialdehyde level measurement using the thiobarbituric acid reactive substance method measured by UV-Visible spectrophotometer at a wavelength of 532 nm. The left lungs were also harvested for histopathological examination. This study showed that pulmonary malondialdehyde levels in second and third group tended to be higher than in first group. Meanwhile, histopathological examination showed that the percentage of lung tissue damage in second and third group tended to be lower than in first group. Thus, it can be concluded that DHEA at doses of 15 and 30 mg/kgBW tended to act as a pro-oxidant but might potentially improve lung histology due to cigarette smoke exposure.

Age-Specific Features of the Levels of Reference Proteins Actin, Tubulin, and GAPDH in Different Organs of Male Wistar Rats.

Western blot analysis is used for evaluation of the level of proteins production in organs and tissues, and housekeeping proteins GAPDH, actin, and tubulin are usually used as the reference proteins. The signal of the target protein is normalized to the corresponding signal of the reference protein. The data on the intensity of actin, tubulin, and GAPDH synthesis are fragmentary: their expression differs in different organs and can vary depending on age, which is often not taken into account in experimental studies. We studied the features of the production of reference proteins in the liver, heart, brain, and lungs of newborn, prepubertal, and adult male Wistar rats. Age-related differences in the expression of β-actin, β-tubulin, and GAPDH in the myocardium and dorsal prefrontal cortex were revealed. GAPDH expression in the dorsal prefrontal cortex in adult rats was significantly higher than in prepubertal rats; GAPDH expression in the myocardium of adult rats was significantly higher than in newborns. The level of actin in the dorsal prefrontal cortex in newborn rats was significantly higher than in prepubertal and adult rats. In the liver and lungs, the expression of actin, tubulin, and GAPDH did not differ in newborn, prepubertal, and adult rats. When choosing the reference protein for Western blotting, animals age and the studied organ should be taken into account.

Antioxidant effect of ethanolic extract of Pleurotus Ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH) level in lung rats exposed to cigarette smoke

Background: Cigarette smoke can trigger oxidative stress. An alternative to overcome the harmful effects of cigarette smoke is through antioxidants. White oyster mushrooms (Pleurotus Ostreatus) are a source of exogenous antioxidants because many contain active compounds for potential antioxidants such as phenol and flavonoid compounds. The purpose of this study was to analyze the effect of ethanolic extract of Pleurotus ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH), as well as to analyze their correlation in the lung of Wistar male rats exposed to cigarette smoke. Methods: The study was a preclinical experiment conducted on 24 rats divided into four groups. The treatment was carried out for 42 days and antioxidant effects were assessed through levels of HNE and GSH in rat lungs. Groups were divided as follows: I- normal control, II- negative control, III and IV exposed to cigarette smoke for 60 minutes/day. Group III (treatment group) was treated with ethanolic extract of Pleurotus ostreatus at 250 mg/kg BW rat/day, and group IV (comparison group) was treated with N-acetyl cysteine 600 mg /day. Data analysis used was one-way ANOVA and Kruskal Wallis test, and Spearmen rank correlation coefficient test. Results: The results showed that the group receiving ethanolic extract of Pleurotus ostreatus had HNE levels of (44,18 ± 2,09 pg/mL) and GSH (0,04 ± 0,00 pg/mL) protein. This extract significantly increased GSH levels and inhibited the increase of HNE levels. Results of GSH (p≤0.01) showed significant results using one-way ANOVA. Conclusions: The ethanolic extract of P. ostreatus can prevent lipid peroxidation and decrease endogenous antioxidant levels in lung cells exposed to cigarette smoke. Ethanolic extract of Pleurotus ostreatus has good antioxidant potential.