This study aims to determine the optimal design and ascent trajectory for a single-stage hybrid rocket in the context of a lunar ascent mission, with the objective of reaching a 100 km circular orbit on the Moon equatorial plane. A multi-disciplinary optimization problem is formulated, integrating flight design variables and launch vehicle design variables to achieve the best mission scenario. A zero-dimensional internal ballistics model is employed to estimate key parameters such as thrust, mass flow rate, and gross mass of the hybrid rocket, while a three-degree of freedom dynamical model is utilized to simulate the ascent trajectory. The resulting integrated optimization problem is solved using an in-house heuristic algorithm that combines particle swarm and genetic algorithms. Two propellant combinations, liquid oxygen/paraffin-wax and hydrogen peroxide (90%)/high-density polyethylene, are evaluated, considering both their performance in the integrated system and the feasibility of the proposed mission. After conducting a sensitivity analysis to investigate the influence of particle count in the optimization process, results for both propellant combinations are presented. While paraffin and oxygen provide the highest payload ratio, hydrogen peroxide and polyethylene entail a more feasible and flexible design for a lunar mission.