Species Of Luffa Research Articles

Genome Assembly and Structural Variation Analysis of Luffa acutangula Provide Insights on Flowering Time and Ridge Development.

Luffa spp. is an important worldwide cultivated vegetable and medicinal plant from the Cucurbitaceae family. In this study, we report a high-quality chromosome-level genome of the high-generation inbred line SG261 of Luffa acutangula. The genomic sequence was determined by PacBio long reads, Hi-C sequencing reads, and 10× Genomics sequencing, with an assembly size of 739.82 Mb, contig N50 of 18.38 Mb, and scaffold N50 of 56.08 Mb. The genome of L. acutangula SG261 was predicted to contain 27,312 protein-coding genes and 72.56% repetitive sequences, of which long terminal repeats (LTRs) were an important form of repetitive sequences, accounting for 67.84% of the genome. Phylogenetic analysis reveals that L. acutangula evolved later than Luffa cylindrica, and Luffa is closely related to Momodica charantia. Comparing the genome of L. acutangula SG261 and L. cylindrica with PacBio data, 67,128 high-quality structural variations (SVs) and 55,978 presence-absence variations (PAVs) were identified in SG261, resulting in 2424 and 1094 genes with variation in the CDS region, respectively, and there are 287 identical genes affected by two different structural variation analyses. In addition, we found that the transcription factor FY (FLOWERING LOCUS Y) families had a large expansion in L. acutangula SG261 (flowering in the morning) compared to L. cylindrica (flowering in the afternoon), which may result in the early flowering time in L. acutangula SG261. This study provides valuable reference for the breeding of and pan-genome research into Luffa species.

Analyzing genetic diversity in luffa and developing a Fusarium wilt-susceptible linked SNP marker through a single plant genome-wide association (sp-GWAS) study

BackgroundLuffa (Luffa spp.) is an economically important crop of the Cucurbitaceae family, commonly known as sponge gourd or vegetable gourd. It is an annual cross-pollinated crop primarily found in the subtropical and tropical regions of Asia, Australia, Africa, and the Americas. Luffa serves not only as a vegetable but also exhibits medicinal properties, including anti-inflammatory, antidiabetic, and anticancer effects. Moreover, the fiber derived from luffa finds extensive applications in various fields such as biotechnology and construction. However, luffa Fusarium wilt poses a severe threat to its production, and existing control methods have proven ineffective in terms of cost-effectiveness and environmental considerations. Therefore, there is an urgent need to develop luffa varieties resistant to Fusarium wilt. Single-plant GWAS (sp-GWAS) has been demonstrated as a promising tool for the rapid and efficient identification of quantitative trait loci (QTLs) associated with target traits, as well as closely linked molecular markers.ResultsIn this study, a collection of 97 individuals from 73 luffa accessions including two major luffa species underwent single-plant GWAS to investigate luffa Fusarium wilt resistance. Utilizing the double digest restriction site associated DNA (ddRAD) method, a total of 8,919 high-quality single nucleotide polymorphisms (SNPs) were identified. The analysis revealed the potential for Fusarium wilt resistance in accessions from both luffa species. There are 6 QTLs identified from 3 traits, including the area under the disease progress curve (AUDPC), a putative disease-resistant QTL, was identified on the second chromosome of luffa. Within the region of linkage disequilibrium, a candidate gene homologous to LOC111009722, which encodes peroxidase 40 and is associated with disease resistance in Cucumis melo, was identified. Furthermore, to validate the applicability of the marker associated with resistance from sp-GWAS, an additional set of 21 individual luffa plants were tested, exhibiting 93.75% accuracy in detecting susceptible of luffa species L. aegyptiaca Mill.ConclusionIn summary, these findings give a hint of genome position that may contribute to luffa wild resistance to Fusarium and can be utilized in the future luffa wilt resistant breeding programs aimed at developing wilt-resistant varieties by using the susceptible-linked SNP marker.

Effects of Different Treatments on Seed Germination and Breaking Seed Dormancy in Wild Luffa Species

Comprehending general combining ability (GCA) and specific combining ability (SCA) is pivotal in maize breeding for successful hybrid development. GCA signifies the overall genetic worth of a parent, while SCA demonstrates unique interactions between parents. In instances where GCA variances surpass SCA variances, early assessments become more effective, enabling the anticipation of superior hybrids based on parental GCA impacts. Assessing the combining ability of maize becomes increasingly crucial with escalating temperatures, which can adversely affect maize growth and crop yield. Research conducted in Prayagraj involved utilizing 7x7 half diallel crosses of maize initially developed during the Rabi 2022 season, followed by their subsequent planting in a randomized block design (RBD) during spring 2023. The primary aim of the study was to evaluate the general combining ability (GCA) and specific combining ability (SCA) effects exhibited by the parents and hybrids, respectively, in order to identify superior-performing parents and hybrids among them. The results showcased strong positive GCA in MILCT-145F (P7) and MILC-2050 (P6), while specific heat-tolerant hybrids, such as P7xP1, P6xP2 exhibited outstanding performance in grain yield and other associated traits.

Symptoms of Nitrogen and Iron Deficiency in Luffa

Luffa (Luffa aegyptiaca, synonym Luffa cylindrica) is a tropical and subtropical climbing plant belonging to the cucumber family (Cucurbitaceae), natively cultivated in South and Southeast Asia. There are two main species of luffa grown in Florida: smooth luffa (Luffa aegyptiaca Mill.), primarily grown in small gardens and angled luffa (Luffa acutangula (L.) Roxb.), basically grown for commercial production (https://edis.ifas.ufl.edu/publication/HS1285). It is commonly called sponge gourd, Egyptian cucumber, or Vietnamese luffa. Young fruits are edible and rich with nutritional and medicinal value (Partap et al. 2012; Azeez, Bello, and Adedeji et al. 2013). The mature fruit has strong fibrous insides, which can be used as natural porous fiber (Siqueira, Bras, and Dufresne 2010; Sivakandhan et al. 2020) for dishwashing or shower scrubbing. This publication is written for growers, state and county Extension faculty, and students interested in crop production.

Simple cryopreservation protocol for Luffa pollen: enhancing breeding efficiency.

This study aimed to develop a long-term pollen storage protocol for Luffa species (L. acutangula, L. cylindrica, L. echinata, and L. graveolens) and assess its potential for crop improvement. The optimal medium for in vitro pollen germination varied by species, with Brewbaker and Kwack (BK) medium with 10% sucrose suitable for L. acutangula, L. cylindrica, and L. echinata, and BK medium with 3% sucrose ideal for L. graveolens. Overestimation in staining tests compared to in vitro pollen germination was observed. The best results for cryopreservation were achieved with desiccation periods of 20, 30, and 40min, maintaining moisture content between 14.04% and 18.55%. Pollen viability was negatively correlated with storage temperature (25, 4, and -20°C) and duration. Cryopreserved pollen at -196°C exhibited the highest viability over a prolonged period (2 months) and was comparable to fresh pollen in terms of germination, ovule fertilization, and fruit and seed set. This study presents a simple and reproducible pollen cryopreservation protocol applicable across Luffa species, facilitating long-term storage and its use in crop improvement efforts.

Novel sources of resistance to fusarium wilt in Luffa species.

Fusarium wilt is a serious disease of cucurbit crops including cultivated Luffa species (Luffa aegyptiaca, Luffa acutangula) causing considerable amount of reduction in yield and quality. Luffa is starting to be used as rootstocks for major commercial cucurbit crops, but little is known of its resistance against soilborne diseases. Here, 63 Luffa accessions from the World Vegetable Center genebank were evaluated for resistance to an aggressive isolate of Fusarium oxysporum f. FoCu-1 (Fsp-66). According to visual screening based on disease severity rating, 14 accessions exhibited a high level of resistance against Fsp-66. These accessions were further evaluated for resistance against Fsp-66 and two more isolates FoCu-1 (isolated from infected cucumber plants) and FoM-6 (isolated from infected bitter gourd plants). Of the 14 accessions, 11 were confirmed resistant against isolate Fsp-66. In addition, 13 accessions showed high resistance against isolates FoCu-1 and FoM-6. This is the first report of Fusarium wilt resistance in Luffa and these sources will be valuable for the development of Luffa rootstocks/cultivars resistant to soil-borne pathogen to manage this serious disease.

Inheritance and molecular mapping of solitary/cluster fruit-bearing habit in Luffa.

Fruiting behaviour and sex form are important goals for Luffa breeders and this study aimed to shed light upon inheritance patterns for both these traits. The hermaphrodite form of Luffa acutangula (known as Satputia) is an underutilized vegetable with a unique clustered fruiting habit. Its desirable traits, such as plant architecture, earliness, as well as contrasting traits like unique clustered fruiting, bisexual flower, and crossability with Luffa acutangula (monoecious ridge gourd with solitary fruits), make it a potential source for trait improvement and mapping of desirable traits in Luffa. In the present study, we have elucidated the inheritance pattern of fruiting behaviour in Luffa using F2 mapping population generated from a cross between Pusa Nutan (Luffa acutangula, monoecious, solitary fruiting) × DSat-116 (Luffa acutangula, hermaphrodite, cluster fruiting). In F2 generation, the observed distribution of plant phenotypes fitted in the expected ratio of 3:1 (solitary vs cluster) for fruit-bearing habit. This is the first report of monogenic recessive control for cluster fruit-bearing habit in Luffa. Herein, we designate for the first time the gene symbol cl for cluster fruit bearing in Luffa. Linkage analysis revealed that SRAP marker ME10 EM4-280 was linked to the fruiting trait at the distance of 4.6cM from the Cl locus. In addition, the inheritance pattern of hermaphrodite sex form in Luffa was also studied in the F2 population of Pusa Nutan×DSat-116 that segregated into 9:3:3:1 ratio (monoecious:andromonoecious:gynoecious:hermaphrodite), suggesting a digenic recessive control of hermaphrodite sex form in Luffa, which was further confirmed by the test cross. The inheritance and identification of molecular marker for cluster fruiting trait provides a basis for breeding in Luffa species.

A SNP of betaine aldehyde dehydrogenase (BADH) enhances an aroma (2-acetyl-1-pyrroline) in sponge gourd (Luffa cylindrica) and ridge gourd (Luffa acutangula)

Luffa is a genus of tropical and subtropical vines belonging to the Cucurbitaceae family. Sponge gourd (Luffa cylindrica) and ridge gourd (Luffa acutangula) are two important species of the genus Luffa and are good sources of human nutrition and herbal medicines. As a vegetable, aromatic luffa is more preferred by consumers than nonaromatic luffa. While the aroma trait is present in the sponge gourd, the trait is not present in the ridge gourd. In this study, we identified Luffa cylindrica’s betaine aldehyde dehydrogenase (LcBADH) as a gene associated with aroma in the sponge gourd based on a de novo assembly of public transcriptome data. A single nucleotide polymorphism (SNP: A > G) was identified in exon 5 of LcBADH, causing an amino acid change from tyrosine to cysteine at position 163, which is important for the formation of the substrate binding pocket of the BADH enzyme. Based on the identified SNP, a TaqMan marker, named AroLuff, was developed and validated in 370 F2 progenies of the sponge gourd. The marker genotypes were perfectly associated with the aroma phenotypes, and the segregation ratios supported Mendelian’s simple recessive inheritance. In addition, we demonstrated the use of the AroLuff marker in the introgression of LcBADH from the aromatic sponge gourd to the ridge gourd to improve aroma through interspecific hybridization. The marker proved to be useful in improving the aroma characteristics of both Luffa species.

Screening Luffa germplasm and advanced breeding lines for resistance to Tomato leaf curl New Delhi virus

The two cultivated Luffa species can be severely infected by Tomato leaf curl New Delhi virus (ToLCNDV) with up to 100% yield loss. Here, 52 Luffa genotypes were screened for ToLCNDV resistance after natural field infection. Mean vulnerability index (VI) ranged from 0.00 to 75.33; genotypes IIHR-137 and IIHR-138 had no symptoms (VI 0), 16 genotypes were resistant (VI 0–25), 15 were moderately resistant (VI 26–50), and 19 were moderate to susceptible (VI > 50). Ten of the most resistant genotypes and five susceptible checks were then challenge-inoculated using whiteflies or sap in an insect-proof net house; only IIHR-137 [L. cylindrica (L.) Roem.] was symptomless (VI 0.00), and 3–5% of plants of IIHR-138 [L. cylindrica (L.) Roem.] and IIHR-Sel-1 [L. acutangula (L.) Roxb.] had only mild symptoms; genotype Arka Prasan was most susceptible (VI 80.96). Asymptomatic plants were confirmed as infected using polymerase chain reaction. Susceptible genotypes rapidly developed leaf curling, then a severe mosaic 10 days post-inoculation. The resistant inbred lines identified are good candidates for a breeding program for ToLCNDV-resistant cultivars.

Genetic analysis of yellow mosaic disease resistance in loofah

Yellow mosaic disease caused by tomato leaf curl New Delhi virus is very severe and causing 100 per cent yield loss under epiphytotic conditions. Chemical control of this disease is neither economical nor eco-friendly and genetic resistance is an efficient means for its management. Resistance to yellow mosaic disease in advanced inbred line IIHR-Sel-1 and IIHR-137 have been confirmed during screening experiment. A crossing programme was planned using Arka Prasan as susceptible and IIHR-Sel-1 and IIHR-137 as resistant parents. We used the F1, F2, BC1 and BC2 generations derived from the two crosses viz. Arka Prasan × IIHR-Sel-1 and Arka Prasan × IIHR 137 to study the inheritance of resistance to yellow mosaic disease. Results indicated monogenic recessive resistance to yellow mosaic disease in the genetic background of both parents which need to be confirmed through repeated experiments with large population size. These results are imperative for breeding yellow mosaic disease resistant cultivars in Luffa species.

De novo assemblies of Luffa acutangula and Luffa cylindrica genomes reveal an expansion associated with substantial accumulation of transposable elements.

Luffa spp. (sponge gourd or ridge gourd) is an economically important vegetable crop widely cultivated in China, India and Southeast Asia. Here, we employed PacBio long-read single-molecule real-time (SMRT) sequencing to perform de novo genome assemblies of two commonly cultivated Luffa species, L.acutangula and L.cylindrica. We obtained preliminary draft genomes of 734.6Mb and 689.8Mb with scaffold N50 of 786,130 and 578,616 bases for L.acutangula and L.cylindrica, respectively. We also applied long-range Chicago and HiC techniques to obtain the first chromosome-scale whole-genome assembly of L.acutangula. The final assembly contained 13 pseudomolecules, corresponding to the haploid chromosome number in Luffa spp. (1n=13, 2n=26). The sizes of the assembled Luffa genomes are approximately twice as large as the genome assemblies of related Cucurbitaceae. A large proportion of L.acutangula (62.17%; 456.69Mb) and L.cylindrica (56.78%; 391.65Mb) genome assemblies contained repetitive elements. Phylogenetic analyses revealed that the substantial accumulation of transposable elements likely contributed to the expansion of the Luffa genomes. We also investigated alternative splicing events in Luffa using full-length transcript sequences obtained from PacBio Isoform Sequencing (Iso-seq). While the predominant form of alternative splicing in most plant species examined was intron retention, alternative 3' acceptor site selection appeared to be a major event observed in Luffa. High-quality genome assemblies for L.acutangula and L.cylindrica reported here provide valuable resources for Luffa breeding and future genetics and comparative genomics studies in Cucurbitaceae.

Long-read sequencing and de novo assembly of the Luffa cylindrica (L.) Roem. genome.

Sponge gourd (Luffacylindrica (L.) Roem.) or luffa is a diploid herbaceous plant with 26 chromosomes (2n=26) and belongs to the family Cucurbitaceae. To address the limited knowledge of the genome of Luffa species, the chromosome-level genome of L.cylindrica was assembled and analysed using PacBio long reads and Hi-C data. We combined Hi-C data with a draft genome assembly to generate chromosome-length scaffolds. Thirteen scaffolds corresponding to the 13 chromosomes were assembled from 1,156 contigs to a final size of 669Mb with a contig N50 size of 5Mb and a scaffold N50 size of 53Mb. After removing redundant sequences, 416.31Mb (62.18% of the genome) of repeat sequences was detected. Subsequently, 31,661 protein-coding genes with an average of 5.69 exons per gene were identified in the L.cylindrica genome using de novo methods, transcriptome data and homologue-based approaches. In addition, 27,552 protein-coding genes (87.02%) were annotated in five databases. According to the phylogenetic analysis, L.cylindrica is closely related to Cucurbita and Cucumis species and diverged from their common ancestor ~28.6-67.1 million years ago. Genome collinearity analysis was performed in Cucurbita moschata, Cucumis sativus and L. cylindrica, and it demonstrated a high degree of conserved gene order in these three species. The completeness of the genome will provide high-quality genomic knowledge on breeding and reveal genetic variation in L.cylindrica.

Identification and optimization of ISSR primers for analysis of genetic variability among different landrace collections of Indian sponge gourd (Luffa cylindrica L.)

Luffa cylindrica L. or sponge gourd is an economically important horticultural crop from Indian origin and belongs to family Cucurbitaceae. It has extremely variable characteristics like fruit shape, size, quality etc. Though, the genetic variety and population structure in Luffa species is not well implicited neither previously reported accurately. Thus, present work aims to optimize methodology for identification of genetic diversity and population structure among its different varieties and landrace assortments using PCR based (ISSR) markers. The study will help us in designing investigation for large scale characterization of genetic variability across different L. cylindrica. germplasm plus gene pool which could benefit breeding programs. In the present work ISSR markers were used in genetic profiling of 58 sponge gourd accessions using five selected ISSR primers. The landrace collection which were giving maximum amplification were then phylogenetically analyzed to identify their closest relationship with other varieties of Sponge gourd. The three landrace (Luffa-886, Luffa-889 and Luffa-890) germplasm were carefully chosen based on their polymorphic results and phylogenetic relationship were established. This study also provides information for Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, evolutionary history and marker-assisted selection in Luffa species.

Pathogenic potential, parasitic success and host efficiency of Meloidogyne incognita and M. javanica on cucurbitaceous plant genotypes

The response to Meloidogyne incognita and M. javanica of 29 cucurbitaceous genotypes belonging to the genera Cucurbita, Lagenaria and Luffa was evaluated to determine their relative host suitability and utility as rotational crops in sustainable agriculture. The pathogenic potential, parasitic success and host efficiency were estimated based on the ability of the nematode to form galls and generate egg masses on selected genotypes. Meloidogyne incognita showed higher pathogenic potential (gall formation) than M. javanica across all genotypes except for C. argyrosperma. However, M. javanica had higher parasitic success (egg mass production) than M. incognita on the Cucurbita genotypes. By contrast, on Lagenaria and Luffa, M. incognita had higher pathogenic potential and parasitic success than M. javanica. All genotypes except Luffa spp., showed higher host efficiency (rate of egg masses per gall) for M. javanica than for M. incognita. The genotypes of Cucurbita pepo ssp. pepo will be useful for M. incognita management because they reduce population build-up, but root damage would be more severe due to abundant root galling. The Cucurbita genotypes would be more tolerant to higher Pi when infected by M. javanica as they suffer less root damage, but the residual populations may affect the subsequent crop in the rotation. Lagenaria siceraria showed a good level of resistance to M. javanica and will be useful by itself or for grafting other cucurbitaceous crops. The species of Luffa were poor hosts of M. javanica and can be used also for grafting.

Microsatellite analysis of genetic diversity and population structure of hermaphrodite ridge gourd (Luffa hermaphrodita).

Out of 103 microsatellite markers used for studying the genetic diversity among local landraces of Luffa species, 56 were found polymorphic, including 38 gSSR and 18 eSSR, respectively. A total of 197 amplification products were obtained. The mean number of alleles per locus was 3.52. The PIC ranged from 0.037 to 0.986, while size of amplified product ranged from 105 to 500bp. Cucumber-derived SSRs were amplified within L. acutangula (68%), L. aegyptiaca (61.16%), and L. hermaphrodita (60.2%), with an average of 63.12% cross-transferability. The Jaccard's coefficient ranged from 0.66 to 0.97, with an average of 0.81. High genetic variability was observed for node of 1st hermaphrodite flower (6.4-17), days to 1st hermaphrodite flower (38-52.1), days to 1st fruit harvest (43-65), number of fruit per cluster (1-5.9), fruit length (3.9-25cm), fruit weight (18.4-175g), number of fruit per plant (20-147.5), and yield per plant (2.2-4.7kg). Two sub-populations were identified including 21 genotypes (sub-population I) and 06 genotypes (sub-population II), these two sub-populations showed 0.608-0.395% of the ancestral relationship to each other. This study provides information for future exploration, collection, and utilization of Luffa genotypes, as well as the polymorphic markers identified could be available for the study of landmarks in linkages, genomic structures, evolutionary ecology, and marker-assisted selection (MAS) in Luffa species.

Genome Survey Sequencing of Luffa Cylindrica L. and Microsatellite High Resolution Melting (SSR-HRM) Analysis for Genetic Relationship of Luffa Genotypes.

Luffa cylindrica (L.) Roem. is an economically important vegetable crop in China. However, the genomic information on this species is currently unknown. In this study, for the first time, a genome survey of L. cylindrica was carried out using next-generation sequencing (NGS) technology. In total, 43.40 Gb sequence data of L. cylindrica, about 54.94× coverage of the estimated genome size of 789.97 Mb, were obtained from HiSeq 2500 sequencing, in which the guanine plus cytosine (GC) content was calculated to be 37.90%. The heterozygosity of genome sequences was only 0.24%. In total, 1,913,731 contigs (>200 bp) with 525 bp N50 length and 1,410,117 scaffolds (>200 bp) with 885.01 Mb total length were obtained. From the initial assembled L. cylindrica genome, 431,234 microsatellites (SSRs) (≥5 repeats) were identified. The motif types of SSR repeats included 62.88% di-nucleotide, 31.03% tri-nucleotide, 4.59% tetra-nucleotide, 0.96% penta-nucleotide and 0.54% hexa-nucleotide. Eighty genomic SSR markers were developed, and 51/80 primers could be used in both “Zheda 23” and “Zheda 83”. Nineteen SSRs were used to investigate the genetic diversity among 32 accessions through SSR-HRM analysis. The unweighted pair group method analysis (UPGMA) dendrogram tree was built by calculating the SSR-HRM raw data. SSR-HRM could be effectively used for genotype relationship analysis of Luffa species.

Reproductive efficiency of two Luffa species - Factors affecting low reproductive rate in meiotically stable Luffa acutangula (L.) Roxb.

Two species of genus Luffa, namely L. cylindrica and L. acutangula have been analyzed during the present study for the details of morphological and meiotic variability. While the former is found only in cultivation, the latter is cultivated on a small scale and also grows in wild in Jammu district of J&K state (India). These species match in their gross morphology and in having a diploid chromosome number of 2n = 26. Of the two, L. acutangula is more efficient in its meiotic system displaying minimum abnormalities and showing higher pollen viability (70.64 %) as compared to L. cylindrica which has slightly abnormal meiosis resulting in lower pollen viability (30.34 %). Inspite of this low pollen viability, L .cylindrica is reproductively much efficient than L. acutangula in terms of % age fruit and seed set and fruit size. Latter lags behind because of some disadvantages in reproductive features including size of flower, time of anthesis, availability of pollinators and rewards offered. All these factors lead to limited pollen transfer resulting in dip in reproductive efficiency.

Population structure and diversity in cultivated and wild Luffa species

Population structure and diversity in cultivated and wild Luffa species

Revisiting <I>Luffa</I> (Cucurbitaceae) 25 Years After C. Heiser: Species Boundaries and Application of Names Tested with Plastid and Nuclear DNA Sequences

Abstract The circumscription of the five to eight species of Luffa, as well as their correct names, have long been problematic. Experts on the genus, most recently C. Heiser and C. Jeffrey, have disagreed on the number of species in the New World and the application of the name L. operculata, which in turn affected the names L. quinquefida and L. sepium. Heiser used classic biosystematic methods, including experimental crossing, to infer species boundaries, but neither researcher had today's option of using DNA sequences for this purpose. We sequenced 51 accessions of Luffa, representing the geographic range of the genus and as much as possible topotypical or type material. Phylogenies from four non-coding plastid regions and the nuclear ribosomal DNA spacer region show that eight clades of specimens have geographic-morphological coherence. Heiser's view that Luffa has three species in the New World is supported, and there are four species in tropical and subtropical Asia. Australia has an endemic species...

Morphological variability in cultivated and wild species of Luffa (Cucurbitaceae) from India

Immature fruits of cultivated species of Luffa are commonly used as summer vegetable in India. Rich morphological variability occurs in cultivated species of Luffa in different growing regions of the country. To study morphological variability in cultivated and wild Luffa from different agro-ecological regions of India, a total of seventy accessions were assembled. Morphological variations in leaf, fruit and seed characters were found to delimit cultivated and wild Luffa species. Cluster analysis revealed two major clusters, one comprising of 36 accessions of Luffa acutangula and the other of 30 accessions belonging to L. aegyptiaca. A local cultivar “Satputia” (L. hermaphrodita) was classified along with cultivated L. acutangula and wild L. acutangula var. amara. Wild species namely L. graveolens and L. echinata were clustered closer to the L. acutangula.