The flow field design of the non-aqueous redox flow battery (RFB) decides the electrolyte flow and mass transfer of active species inside porous electrode, which has a significant influence on the operation of RFB. In this work, a fractal tree-like flow channel is proposed to enhance the electrolyte convection and optimize the concentration distribution of active species inside the graphite felt electrode, thus boosting the overall battery performance. Using the finite element simulation method, a three-dimensional numerical model of single cell is established to comparatively study the steady-state discharge process characteristics of deep eutectic solvent (DES) electrolyte-based vanadium‑iron RFBs with fractal and serpentine flow fields, respectively. Moreover, by assembling the single cell of this RFB and building the full cell test system, an experimental study is conducted to compare the discharging output voltage and power of the RFBs with serpentine flow field and fractal flow fields of different fractal dimensions. The numerical results show that the fractal tree-like flow field can improve the discharging power of RFB as well as reduce the pumping loss compared with the conventional serpentine flow field. That is mainly attributed to that fractal flow field is conducive to reducing the polarization loss and strengthening the convection in porous electrode simultaneously. What's more, the role of fractal dimension of flow field is also investigated numerically, thus knowing that large fractal dimension promotes lower pumping loss while small fractal dimension is in favor of higher output voltage. In addition, the experimental results also demonstrate that the fractal flow field could improve the output voltage and maximum discharging power of RFB compared to serpentine flow field. This work provides an optimization method for the flow field design of DES electrolyte-based vanadium‑iron RFB, and is expected to be employed in the other non-aqueous RFBs.