Lower Green Bay Research Articles

Impacts of Tributary Inflows on the Circulation and Thermal Regime of the Green Bay Estuary of Lake Michigan

One of the largest freshwater estuarine systems on Earth, Green Bay receives freshwater runoff from its land watershed and cold-water intrusions from Lake Michigan. Southern Green Bay is a designated area of concern (AOC) due to ecosystem degradation, contaminated sediments, and poor water quality. Successful restoration of aquatic systems requires a clear understanding of their circulation and thermal regimes. Using a state-of-the-art numerical ocean model, this study examines the effect of variability in tributary inflows and the related lake intrusions on the circulation and thermal regime in Green Bay and on the mass and heat fluxes between lower- and upper–Green Bay areas. Our findings indicate that tributaries influence the circulation and thermal regime, locally and throughout the bay, and that the impact is more significant in hydrologically wet years. Model simulations showed evidence of small net transport out of lower Green Bay. This analysis of the simultaneous effects of tributary flows and lake intrusions in Green Bay can contribute to understanding the dynamics of freshwater estuaries and improve the planning of future restoration projects.

Estimating population size and survival of adult northern pike (Esox lucius) in Lower Green Bay

AbstractComplex life history behaviours like spawning migrations can complicate population assessments. However, predictable aggregation of populations at certain times of year (e.g. at spawning grounds) presents an effective way to sample species that are otherwise spatially widespread. From 2014 to 2019, a mark‐recapture study on Green Bay northern pike (Esox lucius) entering a spawning wetland was used to estimate apparent survival and population size using Bayesian inference and the Cormack–Jolly–Seber model. Population estimates and apparent survival varied from 446 to 1949 and 0.22 (males in 2017) to 0.63 (males in 2015), respectively, among years. Apparent survival also increased with maximum mean daily water temperature. Estimated capture probability was consistently low across years (<30%) despite a sampling method with high capture efficiency of fish present at the wetland, suggesting Green Bay northern pike may be exhibiting low spawning site fidelity or other life history behaviours, like skipped spawning, which reduce their capture probability at a single wetland over time. Further, spawning site selection and timing may respond to water temperatures. Surveying multiple sites could further elucidate important life history traits while refining estimates of population size and structure in large systems.

Assessment of concentration and distribution of total mercury and polychlorinated biphenyls in Green Bay, Wisconsin, USA

Under the 1987 Great Lakes Water Quality Agreement, the lower Green Bay and Fox River estuary have been labeled as areas of concern due to the contamination of mercury and polychlorinated biphenyls (PCBs) from industrialization. These pollutants pose substantial health and environmental hazards for the Green Bay region. The PCBs reported in this region, including Aroclor 1242, are known to trigger carcinogenic responses in animals and mercury targets the central nervous system and vital organs. Furthermore, these compounds are extremely difficult to remove from the environment once introduced. Extensive remedial actions have been implemented including dredging sediments in the Lower Fox River from DePere to Green Bay. The purpose of this study is to assess the concentration and distribution of Aroclor 1242 and total mercury in the Green Bay region sediments and pore waters and to assess the impact of interventions and the natural rates of change previously found.

Seiche- and storm-driven benthic oxygen uptake in a eutrophic freshwater bay determined with aquatic eddy covariance

Oxygen depletion in bottom waters of lakes and coastal regions is expanding worldwide. To examine the causes of hypoxia, we quantified the drivers of benthic oxygen uptake in Green Bay, Lake Michigan, USA, using 2 techniques, aquatic eddy covariance and sediment core incubation. We investigated benthic oxygen uptake along a gradient in C deposition, including shallow water near the riverine source of eutrophication and deeper waters of lower Green Bay where high net sediment deposition occurs. Time-averaged eddy covariance oxygen uptake was high near the source of eutrophication (11.5 mmol m−2 d−1) and at the shallower of the high deposition sites (9.8 mmol m−2 d−1). The eddy covariance technique revealed a decrease in benthic oxygen uptake with depth at the high deposition sites. These patterns were consistent with benthic uptake being driven by the deposition of autochthonous production. Additionally, eddy covariance revealed a nearly proportional relationship between benthic oxygen uptake and current velocity at all sites. Specifically, because of the lake seiche, water velocity typically varied 3× at a site and caused a 3× variation in benthic oxygen uptake. A summer storm also doubled bottom-water velocities and caused a further doubling of uptake to 28 mmol m−2 d−1. This high sensitivity of benthic oxygen uptake to seiche-driven water velocities indicates that redox conditions in surficial cohesive sediments are highly dynamic.

Production and outmigration of young-of-year northern pike Esox lucius from natural and modified waterways connected to Lower Green Bay, Wisconsin.

Production and outmigration of young-of-year (YOY) northern pike from natal sites in Lower Green Bay, WI, USA, were documented over three consecutive years (2013-2015). We tested the hypothesis that spawning success and outmigration characteristics of YOY northern pike would vary among natural and anthropogenically modified habitats. Sixteen focal study locations were surveyed, including a restored natural wetland, agricultural drainage ditches, a flooded forested wetland and several unimpounded tributaries. We collected 1469 YOY northern pike with most individuals (N= 1163) originating from a flooded forested wetland on the east shore. Most sites produced YOY in all years (range N= 2-1145 individuals among study years). Outmigration ranged between 1 and 40 days during 2013-2015. Greater production and extended outmigration times occurred at most sites in 2014 (range 17-40 days) when the region experienced a late spring with heavy precipitation. In contrast, the lowest production and shortest outmigration period occurred at most sites in 2015 (range 14-23 days) when environmental conditions reflected regional averages. Outmigration began nearly 3 weeks earlier in 2015 (5/8) than in other study years (8 June 2013 and 25 May 2014). Total length (TL) of outmigrating northern pike ranged between 17 and 138 mm. Total length of YOY was significantly different among sites in 2013 and 2014, with the smallest fish (17 mm TL) outmigrating from agricultural ditches in both years. There were no significant variations in size among sites in 2015 (range 21-95 mm TL). Our results indicate significant variation in YOY northern pike outmigration characteristics within Lower Green Bay that may reflect the interplay between adult spawning site selection and annual weather patterns. Our findings highlight the importance of quantifying overlooked habitats in regions of mixed development.

Timescales of transport through Lower Green Bay

Contaminated sediments, poor water quality, and lost or altered habitat in Lower Green Bay and Fox River in the 1980s led to its listing as an Area of Concern by the International Joint Commission. Previous studies on the geophysics and health of the bay demonstrated the need for estimates of transport timescales. Such estimates can contribute to improved understanding of the transport and fate of nutrients, contaminants, and biogeochemical processes in the bay. This study reviews definitions of residence time and flushing time, estimates the temporal distribution of flushing time, the spatial and temporal distributions of residence time, and horizontal diffusivities. The study used a previously developed hydrodynamic model, a drifter experiment, a lake particle transport model, and methods appropriate for the estimation of transport timescales. The estimated residence time for lower Green Bay, 56 ± 16 days, is smaller than a previous estimate of 190 days for the whole bay, and had values similar to the estimated flushing times. The closeness between those timescales demonstrates the important role of water exchange across the Chambers Island transect. Flushing time and residence time do not follow the same trends in monthly variability because the former depends directly on water exchange across Chambers Island, while the later depends, in addition, on tributary inflows and the circulation patterns in the bay. The study includes a discussion of the relations between the timescales of transport and the previous studies of biogeochemical processes, such as trophic conditions, spatial distribution of cyanobacteria, cold-water intrusions, and hypoxia.

Absence of PCB Hot Spot Effect in Walleye Sander vitreus from Lower Green Bay of Lake Michigan.

Under certain conditions, polychlorinated biphenyl (PCB) concentration in individuals of one sex of an adult fish population may exceed that of the other sex by more than a factor of two. This phenomenon, known as the PCB hot spot effect, has been postulated to be contingent upon the following two conditions: (1) presence of a PCB hot spot in the bottom sediments of the aquatic ecosystem, such that prey PCB concentrations in the hot spot region are substantially higher than prey PCB concentrations in locations distant from the hot spot, and (2) habitat use varying between the sexes, such that individuals of one sex inhabit the hot spot region to a considerably greater degree than individuals of the other sex. To test whether PCB concentrations in walleye Sander vitreus from lower Green Bay of Lake Michigan displayed a PCB hot spot effect, whole-fish PCB concentrations were determined in ten female and ten male adult walleye from the population spawning in the Fox River, the main tributary to lower Green Bay. In addition, mark-recapture data for the Fox River walleye population were analyzed to determine differences in spatial distributions between the sexes. Results revealed that the ratio of mean PCB concentration in males to mean PCB concentration in females was only 1.13, indicating the absence of a PCB hot spot effect. This result was corroborated by the mark-recapture data analysis, which showed no significant difference in habitat use between the sexes. Thus, although condition 1 was met, condition 2 was not met. Consequently, the PCB hot spot effect was not observed in the Fox River walleye population. Lack of a significant difference in PCB congener distributions between the sexes further corroborated our conclusions.

Mercury Isotopes Reveal an Ontogenetic Shift in Habitat Use by Walleye in Lower Green Bay of Lake Michigan

In general, fish residing in rivers differ from fish residing in lakes in their mercury (Hg) isotope ratios. Specifically, fish residing in lakes typically show enriched values for the isotope rati...

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The Laurentian Great Lakes have had numerous introductions of non-indigenous fishes. Some of these species became invasive, resulting in negative economic and ecological impacts. Given the vulnerability of the Great Lakes to future introductions, monitoring for non-indigenous fishes is necessary to protect the Great Lakes ecosystem. This manuscript describes the adaptive development (2013–2017) and results of an early detection and monitoring (EDM) program for non-indigenous fishes in lower Green Bay and the Fox River, a high-risk location for species introductions in Lake Michigan and a potential vector between the Great Lakes and inland ecosystems. The adaptive management approach to EDM has been a continuous cycle of refining sampling gears and methods to improve monitoring each successive year and determining a new method to assess survey performance (determining the contemporary fish community). To date, no non-indigenous fishes previously unknown to the Great Lakes have been detected. Survey performance (i.e., ability to capture species in the fish communty) of this EDM program has increased since its inception in 2013, and a target of 90% survey performance was achieved or nearly met in 2014–2017. Gears and methods will continue to be adaptively refined; however, the current sampling regime and survey performance should provide effective early detection of new non-indigenous fishes, allowing managers to respond early in the introduction phase, when management actions may be more effective.

The Green Bay saga: Environmental change, scientific investigation, and watershed management

Abstract The Green Bay watershed, draining a total area of approximately 40,468 km2, comprises about a third of the Lake Michigan drainage. In the early years, fur trade was the dominant economic activity within the watershed. Later, when timber harvesting, papermaking, and agriculture came on the scene in the 19th and early 20th centuries, major environmental changes occurred in a relatively short period of time. Nutrient and sediment loadings, accompanied by organic wastes from sawmills and paper mills, resulted in a pollutant overload in the Fox River and in the eutrophication of the waters of lower Green Bay. Citizen complaints about these severely degraded conditions initiated a period of scientific investigation. Starting slowly with a few studies and surveys in the first half of the 20th century, serious investigatory work began at mid-century with support from the University of Wisconsin Sea Grant Institute. Examples of topics that have been investigated since then with support from numerous sources are: biological oxygen demand (BOD), phosphorus and total suspended solids loads, trophic status and food chain efficiencies, coastal wetland characterization, dynamics of the benthic layer, algae and abiotic solids, phosphorus cycling and mass balance, PCBs, seasonal hypoxia, and climate change impacts. These studies have provided the scientific foundation for government-led programs such as the Green Bay Remedial Action Program, the PCB clean-up program, and the TMDL program. Progress has been made—reduction in BOD is an example—but a fuller rehabilitation of this large-scale ecosystem remains an elusive goal. The saga goes on.

In situ, high-resolution time series of dissolved phosphate in Green Bay, Lake Michigan

Abstract In nearly every instance in which the environment has been sampled on a higher resolution in time or space, fundamental processes have come to light that were previously undetected or unobserved. In this study, an autonomous dissolved phosphate sensor was deployed at the Entrance Light station in lower Green Bay, Lake Michigan in 2012 and 2013. Hourly phosphate sensor measurements were compared with other real time sensor data to gain insights into the processes occurring at this site. Results showed that the water column at this location undergoes repeated stratification and turnover during the course of the summer. Often, the stratification results from intrusions of cold hypolimnetic bottom water from the north, while turnover is associated with significant northerly and/or easterly wind events. It was observed that, during calm periods, dissolved phosphate concentrations increased at a rate that was stoichiometrically consistent with the consumption of dissolved oxygen during the remineralization of organic matter; specifically, areal oxygen consumption rates ranged from 3.2 to 43 mmol m−2 d−1 and oxygen to phosphate ratios ranged from 120 to 210. At other times, the inverse relationship between dissolved oxygen and dissolved phosphate was not stoichiometrically linked; during these times, areal oxygen depletion rates ranged from 51 to 240 mmol m−2 d−1 and oxygen to phosphate ratios ranged from 260 to 2300. Future strategic deployment of multiple in situ dissolved phosphate and other nutrient sensors will enhance the understanding of nutrient cycling in this important aquatic system.

Quantitative restoration targets for fish and wildlife habitats and populations in the Lower Green Bay and Fox River AOC

Abstract The Lower Green Bay and Fox River Area of Concern (LGB&FR AOC) is one of the most ecologically diverse but demonstrably impaired AOCs in the Laurentian Great Lakes. We outline a transparent, quantitative process for setting targets to remove two fish and wildlife-related beneficial use impairments (BUIs). The method identifies important habitats and species/species groups and weights them according to ecological and socioeconomic criteria. These weights are paired with standardized estimates of current condition ranging from 0 (worst possible) to 10 (best possible). A weighted average of the condition scores gives an overall AOC condition for each BUI, creating a baseline for setting future restoration or BUI removal targets. Weighted averages for the LGB&FR AOC yielded a current condition of 3.60 for fish and wildlife habitats and 4.65 for species/species groups. Based on achievable restoration scenarios and discussions with local experts and stakeholders, we propose removal targets of 6.0 for the “loss of fish and wildlife habitat” BUI and 6.5 for the “degradation of fish and wildlife populations” BUI. This quantitative approach illuminates multiple pathways for reaching restoration targets and facilitates informed discussions about cost effective restoration projects. According to our results, species and species groups in this AOC are generally in better current condition than habitats when compared on the same 0–10 scale. This suggests that many (though not all) desirable fish and wildlife populations in the LGB&FR AOC are able to survive in relatively degraded habitats or are able to use these habitats productively during part of their life cycle.

Evaluation of lower Green Bay benthic fauna with emphasis on re-ecesis of Hexagenia mayfly nymphs

Abstract The last historic Hexagenia specimen in lower Green Bay was officially recorded in 1955. Field surveys and Hexagenia viability studies were completed to determine if lower Green Bay could support Hexagenia re-ecesis and where in the bay egg stocking could best be accomplished. The invertebrate field data were compared with historical population data based on earlier published studies in the 1950s, 1970s and 1990s to determine the bay's ecological trajectory to better understand the re-ecesis success potential of Hexagenia. No native Hexagenia were observed during this study. Deep water invertebrate diversity within the upper lower bay appears to be improving, whereas the diversity along the lower mid-bay may be deteriorating. Shallower, nearshore samples indicated a better condition with Caenis mayflies sparsely present, amphipods, isopods, gilled snails, odonates, oligochaetes, chironomids, and meiofauna present. These results suggested improved conditions shoreward versus degraded conditions deeper. Hexagenia egg viability and neonate growth indicated Hexagenia could successfully inhabit in situ Green Bay nearshore (

Development and implementation of an adaptive management approach for monitoring non-indigenous fishes in lower Green Bay, Lake Michigan

Abstract The Laurentian Great Lakes have had numerous introductions of non-indigenous fishes. Some of these species became invasive, resulting in negative economic and ecological impacts. Given the vulnerability of the Great Lakes to future introductions, monitoring for non-indigenous fishes is necessary to protect the Great Lakes ecosystem. This manuscript describes the adaptive development (2013–2017) and results of an early detection and monitoring (EDM) program for non-indigenous fishes in lower Green Bay and the Fox River, a high-risk location for species introductions in Lake Michigan and a potential vector between the Great Lakes and inland ecosystems. The adaptive management approach to EDM has been a continuous cycle of refining sampling gears and methods to improve monitoring each successive year and determining a new method to assess survey performance (determining the contemporary fish community). To date, no non-indigenous fishes previously unknown to the Great Lakes have been detected. Survey performance (i.e., ability to capture species in the fish communty) of this EDM program has increased since its inception in 2013, and a target of 90% survey performance was achieved or nearly met in 2014–2017. Gears and methods will continue to be adaptively refined; however, the current sampling regime and survey performance should provide effective early detection of new non-indigenous fishes, allowing managers to respond early in the introduction phase, when management actions may be more effective.

A Comparison of Three Paired Modified Fyke Nets for Characterizing Fish Assemblages in the Nearshore Zone of Lake Michigan

AbstractWe performed a gear comparison between three types of paired fyke nets used to monitor fish communities in the nearshore zone of Lake Michigan. Our objective was to identify a single net design that would maximize species diversity and catch. The three types of paired fyke nets included a small frame model with 3‐mm bar mesh (Mini‐fyke), a larger‐framed model with 13‐mm mesh (Fyke), and a large frame model with 13‐mm mesh recommended by the American Fisheries Society (AFS‐fyke). We set all three gear types in triplicate on similar nearshore littoral habitat at 16 sites in Green Bay, an embayment of Lake Michigan. Total catch was dominated by Gizzard Shad Dorosoma cepedianum, Round Goby Neogobius melanostomus, Spottail Shiner Notropis hudsonius, Trout‐perch Percopsis omiscomaycus, and Yellow Perch Perca flavescens, which represented 99.3% of all individuals captured. We found that Mini‐fyke nets selected for smaller individuals, caught relatively few species, and had the highest catch per unit effort; this was largely driven by high catches of Spottail Shiner and Gizzard Shad. Fyke nets captured the largest individuals, had the lowest catch per unit effort, and the greatest diversity of species. Results for AFS‐fyke nets were typically intermediate between Mini‐fyke and Fyke nets for most parameters. Species accumulation curves indicated that Fyke nets acquired more total species at a higher rate than the other two net types. Given the need to balance effort, gear bias, and sampling efficiency, we found that AFS‐fyke nets recommended by the AFS effectively characterized the nearshore fish community of lower Green Bay, especially if used in conjunction with complementary sampling gears.Received November 28, 2016; accepted May 25, 2017Published online August 4, 2017

Survival, growth, and production of Hexagenia bilineata mayflies in fluidized sediment from lower Green Bay, Lake Michigan

Abstract Organic pollution in lower Green Bay, Lake Michigan over the past century was accompanied by the local extirpation of Hexagenia (primarily H. limbata ) mayflies. Recoveries were made in other systems where population crashes had occurred (e.g., western Lake Erie); however, an active recovery does not appear to be taking place in Green Bay. Excessive primary production has caused substantial benthic organic matter accumulation resulting in a fluidized “sludge-like” substrate as the majority of the sub-littoral habitat. Fluidized substrate potentially hinders Hexagenia nymphs' abilities to construct and maintain burrows critical to their life cycles. In this study, Hexagenia bilineata nymphs were collected from an Upper Mississippi River backwater where their presence at high densities in relatively fluid sediment had been observed, and reared in oxygenated aquaria containing lower Green Bay or Upper Mississippi River sediment. Their survival, growth, secondary production, and biomass turnover were calculated for a 166 day period. Seventy-five percent of nymphs survived or metamorphosed into winged sub-imagos in lower Green Bay substrates compared to 40.6% in Upper Mississippi River substrates. Nymph dry weight more than tripled in Green Bay substrates and more than doubled on Upper Mississippi River substrates. Production was notably higher in lower Green Bay substrates. Differences in survival and production between the two treatments were statistically significant (P 0.05). Based on these results, the high fluidity of lower Green Bay substrates did not appear to hinder burrow construction or maintenance.

Phytoplankton Communities in Green Bay, Lake Michigan after Invasion by Dreissenid Mussels: Increased Dominance by Cyanobacteria

Biological invasions of aquatic systems disrupt ecological communities, and cause major changes in diversity and ecosystem function. The Laurentian Great Lakes of North America have been dramatically altered by such invasions, especially zebra (Dreissena polymorpha) and quagga (D. rostriformis bugensis) mussels. Responses to mussel invasions have included increased water clarity, and decreased chlorophyll and phytoplankton abundance. Although not all systems have responded similarly, in general, mussels have changed nutrient dynamics and physical habitat conditions. Therefore examination of different impacts can help us further understand mechanisms that underlie ecosystem responses to biological invasions. To aid our understanding of ecosystem impacts, we sampled established locations along a well-studied trophic gradient in Green Bay, Lake Michigan, after the 1993 zebra mussel invasion. A strong trophic gradient remained during the period sampled after the mussel invasion (2000–2012). However, mean summer chlorophyll increased and other measures of phytoplankton biomass (microscope and electronic cell counting) did not change significantly. Multivariate analyses of phytoplankton community structure demonstrate a significant community shift after the invasion. Cyanobacteria increased in dominance, with Microcystis becoming the major summer taxon in lower Green Bay. Diatom diversity and abundance also increased and Chlorophyta became rare. Phytoplankton responses along the trophic gradient of Green Bay to zebra mussel invasion highlight the importance of mussel effects on nutrient dynamics and phytoplankton diversity and function.

Contaminant exposure of birds nesting in Green Bay, Wisconsin, USA.

In earlier studies, elevated concentrations of polychlorinated biphenyl (PCB) and p,p'-dichlorodiphenyldichloroethylene (DDE) were reported in double-crested cormorant (Phalacrocorax auritus) eggs and tree swallow (Tachycineta bicolor) eggs and nestlings collected from lower Green Bay (WI, USA) in 1994 and 1995 and black-crowned night-heron (Nycticorax nycticorax) eggs collected in 1991. Comparable samples collected in 2010 and 2011 indicated that concentrations of PCBs were 35%, 62%, 70%, and 88% lower than in the early 1990s in tree swallow eggs, tree swallow nestlings, double-crested cormorant eggs, and black-crowned night-heron eggs, respectively; concentrations of DDE were 47%, 43%, 51%, and 80% lower, respectively. These declines are consistent with regional contaminant trends in other species. Concentrations of PCBs were higher in herring gull (Larus argentatus) than in black-crowned night-heron eggs collected from Green Bay in 2010; PCB concentrations in double-crested cormorant and tree swallow eggs were intermediate. The estimated toxicity of the PCB mixture in eggs of the insectivorous tree swallow was the equal to or greater than toxicity in the 3 piscivorous bird species. A multivariate analysis indicated that the composition percentage of lower-numbered PCB congeners was greater in eggs of the insectivorous tree swallow than in eggs of the 3 piscivorous species nesting in Green Bay. Dioxin and furan concentrations and the toxicity of these chemicals were also higher in tree swallows than these other waterbird species nesting in Green Bay.

Chloride and total phosphorus budgets for Green Bay, Lake Michigan

Green Bay is an elongated freshwater embayment located in northwestern Lake Michigan. Due to its short residence time, the lower bay is heavily influenced by the Fox River's large nutrient load. The inner bay is classified as hypereutrophic and a well-defined trophic gradient is observed moving away from the Fox River towards Lake Michigan, where the bay is nearly oligotrophic. Recent chloride and total phosphorus loading estimates were used to update a chloride and total phosphorus mass-balance model for the bay for 1994–2008. The chloride model provided a means to estimate turbulent eddy diffusion within the bay and exhibited excellent agreement with observed data. The total phosphorus model agreement with observed data was generally good, with the exception of a large deviation in lower Green Bay during 1999–2004. The model was used to estimate the internal loadings necessary to account for the deviation in phosphorus concentrations. The source of the unexpected increase remains unclear, but we speculate significant internal loading due to wind-driven sediment resuspension and hypoxia-induced phosphorus diffusion was significant. These models allow needed reductions to be identified and sourced and also indicate the role internal loading may play in the Green Bay phosphorus budget.

Spawning Habitat Unsuitability: An Impediment to Cisco Rehabilitation in Lake Michigan?

AbstractThe cisco Coregonus artedi was one of the most important native prey fishes in Lake Michigan and in the other four Laurentian Great Lakes. Most of the cisco spawning in Lake Michigan was believed to have occurred in Green Bay. The cisco population in Lake Michigan collapsed during the 1950s, and the collapse was attributed in part to habitat degradation within Green Bay. Winter water quality surveys of lower Green Bay during the 1950s and 1960s indicated that the bottom dissolved oxygen (DO) concentration was less than 2 mg/L throughout much of the lower bay, and most cisco eggs would not successfully hatch at such low DO concentrations. To determine present‐day spawning habitat suitability in lower Green Bay, we compared cisco egg survival in lower Green Bay with survival at a reference site (St. Marys River, Michigan–Ontario) during 2009. We also conducted winter water quality surveys in lower Green Bay and the St. Marys River during 2009 and 2010. Cisco egg survival in lower Green Bay averaged 65.3%, which was remarkably similar to and not significantly different from the mean at the St. Marys River site (64.0%). Moreover, the lowest bottom DO concentrations recorded during the winter surveys were 11.2 mg/L in lower Green Bay and 12.7 mg/L in the St. Marys River. These relatively high DO concentrations would not be expected to have any negative effect on cisco egg survival. We conclude that winter water quality conditions in lower Green Bay were suitable for successful hatching of cisco eggs and that water quality during the egg incubation period did not represent an impediment to cisco rehabilitation in Lake Michigan. Our approach to determining spawning habitat suitability for coregonids would be applicable to other aquatic systems.Received May 14, 2011; accepted July 6, 2011