Low Acoustic Pressure Research Articles

Low-boiling-point perfluorocarbon nanodroplets for adaptable ultrasound-induced blood-brain barrier opening

Low-boiling point perfluorocarbon nanodroplets (NDs) are valued as effective sonosensitive agents, encapsulating a liquid perfluorocarbon that would instantaneously vaporize at body temperature without the NDs shell. Those NDs have been explored for both therapeutic and diagnostic purposes. Here, phospholipid-shelled nanodroplets containing octafluoropropane (C3F8) or decafluorobutane (C4F10) formed by condensation of microbubbles were thoroughly characterized before blood-brain (BBB) permeabilization. Transmission electron microscopy (TEM) and cryo-TEM were employed to confirm droplet formation while providing high-resolution insights into the droplet surface and lipid arrangement assessed from electron density observation after condensation. The vaporization threshold of NDs was determined with a high-speed camera, and the frequency signal emitted by the freshly vaporized bubbles was analyzed using cavitation detection. C3F8 NDs exhibited vaporization at 0.3 MPa (f0 = 1.5 MHz, 50 cycles), and emitted signals at 2 f0 and 1.5 f0 from 0.45 MPa onwards (f0 = 1.5 MHz, 50 cycles), while broadband noise was measured starting from 0.55 MPa. NDs with the higher boiling point C4F10 vaporized at 1.15 MPa and emitted signals at 2 f0 from 0.65 MPa and 1.5 f0 from 0.9 MPa, while broadband noise was detected starting from 0.95 MPa. Both ND formulations were used to permeabilize the BBB in healthy mice using tailored ultrasound sequences, allowing for the identification of optimal applications for each NDs type. C3F8 NDs proved suitable and safe for permeabilizing a large area, potentially the entire brain, at low acoustic pressure. Meanwhile, C4F10 droplets facilitated very localized (400 μm isotropic) permeabilization at higher pressure. This study prompts a closer examination of the structural rearrangements occurring during the condensation of microbubbles into NDs and highlight the potential to tailor solutions for different brain pathologies by choosing the composition of the NDs and adjusting the ultrasound sequence.

Cavitation Characteristics in Ultrasonic Soldering and the Erosion Effect

In this work, the cavitation characteristics of the solder sonocapillary action during ultrasonic soldering were studied by a high-speed camera. The effects of ultrasonic power and time on the acoustic pressure, cavitation characteristics, and erosion effects were also investigated. Results showed that cavitation occurred throughout the entire sonocapillary action. Two kinds of bubble structures were observed. The steady bubbles had large sizes and long life periods. The transient bubbles had small sizes and short life periods. The steady bubbles preferentially appeared at the primary filling stage, while the transient bubbles dominated the entire cavitation field. The sonocapillary action was divided into the primary filling stage, the intermediate filling stage, and the completion filling stage. The primary filling stage had low acoustic pressure but high bubble density because the air content in the solder was high and some additional air entered the solder through the triple interface caused by ultrasonic vibration. Higher ultrasonic power resulted in stronger cavitation and, thus, better erosion effect on the substrate.

Computational fluid dynamics simulations of single-bubble evolution under synergy of ultrasound and an electrostatic field

In the study reported here, a two-phase flow model was developed for the evolution of a single bubble under ultrasonic irradiation coupled with an electrostatic force. We started with the following assumptions: (I) the liquid is incompressible, (II) the effect of gravity is negligible in the liquid, (III) the bubble is insulating, and no free charges are distributed on the liquid–gas interface, and (IV) the liquid contains only one bubble. Using computational fluid dynamics, we analyzed how the bubble shape evolves under various conditions, and the main findings are as follows: (1) With increasing electric field strength, the bubble reaches a larger maximum area and a smaller minimum area. Furthermore, during the positive phase of ultrasound, a higher electric field strength leads to faster compression and a more slender bubble. (2) As the initial bubble radius is increased from 3 to 5 μm, the cavitation becomes significantly stronger, but when the initial bubble radius reaches 10 μm, the cavitation intensity decreases instead because of greater compression resistance caused by there being more gas in the bubble. (3) Cavitation cannot be triggered under an excessively low acoustic pressure amplitude, and an excessively high acoustic pressure amplitude results in weaker cavitation; the appropriate acoustic pressure amplitude is considered to be 1.35 atm.

Hydrophobin-Coated Perfluorocarbon Microbubbles with Strong Non-Linear Acoustic Response

Gas-filled microbubbles are well-established contrast agents for ultrasound imaging and widely studied as delivery systems for theranostics. Herein, we have demonstrated the promising potential of the hydrophobin HFBII—a fungal amphiphilic protein—in stabilizing microbubbles with various fluorinated core gases. A thorough screening of several experimental parameters was performed to find the optimized conditions regarding the preparation technique, type of core gas, HFBII initial concentration, and protein dissolution procedure. The best results were obtained by combining perfluorobutane (C4F10) gas with 1 mg/mL of aqueous HFBII, which afforded a total bubble concentration higher than 109 bubbles/mL, with long-term stability in solution (at least 3 h). Acoustic characterization of such microbubbles in the typical ultrasound frequency range used for diagnostic imaging showed the lower pressure resistance of HFBII microbubbles, if compared to conventional ones stabilized by phospholipid shells, but, at the same time, revealed strong non-linear behavior, with a significant harmonic response already at low acoustic pressures. These findings suggest the possibility of further improving the performance of HFBII-coated perfluorinated gas microbubbles, for instance by mixing the protein with other stabilizing agents, e.g., phospholipids, in order to tune the viscoelastic properties of the outer shell.

Controlled Hyperthermia With High-Intensity Focused Ultrasound and Ultrasound Contrast Agent Microbubbles in Porcine Liver.

The objective of this work was to study microbubble-enhanced temperature elevation with high-intensity focused ultrasound (HIFU) at different acoustic pressures and under image guidance. The microbubbles were administered with either local or vascular injections (that mimic systemic injections) in perfused and non-perfused ex vivo porcine liver under ultrasound image guidance. Porcine liver was insonified for 30 s with a single-element HIFU transducer (0.9 MHz, 0.413 ms, 82% duty cycle, focal pressures of 0.6-3.5 MPa). Contrast microbubbles were injected either locally or through the vasculature. A needle thermocouple at the focus measured temperature elevation. Diagnostic ultrasound (Philips iU22, C5-1 probe) guided placement of the thermocouple and delivery of microbubbles and monitored the procedure in real time. At lower acoustic pressures (0.6 and 1.2 MPa) in non-perfused liver, inertial cavitation of the injected microbubbles led to greater temperatures at the focus compared with HIFU-only treatments. At higher pressures (2.4 and 3.5 MPa) native inertial cavitation in the tissue (without injecting microbubbles) resulted in temperature elevations similar to those after injecting microbubbles. The heated area was larger when using microbubbles at all pressures. In the presence of perfusion, only local injections provided a sufficiently high concentration of microbubbles necessary for significant temperature enhancement. Local injections of microbubbles provide a higher concentration of microbubbles in a smaller area, avoiding acoustic shadowing, and can lead to higher temperature elevation at lower pressures and increase the size of the heated area at all pressures.

Theranostics in the vasculature: bioeffects of ultrasound and microbubbles to induce vascular shutdown.

Ultrasound-triggered microbubbles destruction leading to vascular shutdown have resulted in preclinical studies in tumor growth delay or inhibition, lesion formation, radio-sensitization and modulation of the immune micro-environment. Antivascular ultrasound aims to be developed as a focal, targeted, non-invasive, mechanical and non-thermal treatment, alone or in combination with other treatments, and this review positions these treatments among the wider therapeutic ultrasound domain. Antivascular effects have been reported for a wide range of ultrasound exposure conditions, and evidence points to a prominent role of cavitation as the main mechanism. At relatively low peak negative acoustic pressure, predominantly non-inertial cavitation is most likely induced, while higher peak negative pressures lead to inertial cavitation and bubbles collapse. Resulting bioeffects start with inflammation and/or loose opening of the endothelial lining of the vessel. The latter causes vascular access of tissue factor, leading to platelet aggregation, and consequent clotting. Alternatively, endothelium damage exposes subendothelial collagen layer, leading to rapid adhesion and aggregation of platelets and clotting. In a pilot clinical trial, a prevalence of tumor response was observed in patients receiving ultrasound-triggered microbubble destruction along with transarterial radioembolization. Two ongoing clinical trials are assessing the effectiveness of ultrasound-stimulated microbubble treatment to enhance radiation effects in cancer patients. Clinical translation of antivascular ultrasound/microbubble approach may thus be forthcoming.

Blood–brain barrier opening in a large animal model using closed-loop microbubble cavitation-based feedback control of focused ultrasound sonication

Focused ultrasound (FUS) in combination with microbubbles has been established as a promising technique for noninvasive and localized Blood–brain barrier (BBB) opening. Real-time passive cavitation detection (PCD)-based feedback control of the FUS sonication is critical to ensure effective BBB opening without causing hemorrhage. This study evaluated the performance of a closed-loop feedback controller in a porcine model. Calibration of the baseline cavitation level was performed for each targeted brain location by a FUS sonication in the presence of intravenously injected microbubbles at a low acoustic pressure without inducing BBB opening. The target cavitation level (TCL) was defined for each target based on the baseline cavitation level. FUS treatment was then performed under real-time PCD-based feedback controller to maintain the cavitation level at the TCL. After FUS treatment, contrast-enhanced MRI and ex vivo histological staining were performed to evaluate the BBB permeability and safety. Safe and effective BBB opening was achieved with the BBB opening volume increased from 3.8 ± 0.7 to 53.6 ± 23.3 mm3 as the TCL was increased from 0.25 to 1 dB. This study validated that effective and safe FUS-induced BBB opening in a large animal model can be achieved with closed-loop feedback control of the FUS sonication.

Large Dynamic Range in Acoustic Detection Realized by a Dual-Diaphragm Based Sensing System With IQ-PGC Phase Demodulation

A dual-diaphragm based acoustic sensing system has been proposed to increase the dynamic range by using a thin diaphragm (70 nm) of gold diaphragm to decrease minimum detectable acoustic pressure and a thick one (300 nm) to increase maximum detectable acoustic pressure. Signals from the two diaphragms are synchronously demodulated using frequency division multiplexing (FDM) combined with an in-phase/quadrature (IQ) and a phase generated carrier (PGC) phase demodulation. Signal crosstalk from the two diaphragms is avoided by adding a carrier modulation frequency shift to one of them. The underwater sensing performance of the system is tested under the acoustic signals ranging from 100 to 1500 Hz. Experimental results demonstrate that the average dynamic range reaches 98.4 dB in 300 ~ 1500 Hz, which is obviously improved comparing to the sensor barely using a 70 nm diaphragm (81.2 dB) or a 300 nm one (74.2 dB). In addition, the sensor performs high and flat sensitivity under low acoustic pressure (less than 110 Pa) with an average phase sensitivity of −164.2±2.6 dB re 1 rad/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$\mu $ </tex-math></inline-formula> Pa. Due to the characteristic of large dynamic range and compact size, the proposed sensing system is believed to have potential application prospects in acoustic detection area.

An enhanced, rational model to study acoustic vaporization dynamics of a bubble encapsulated within a nonlinearly elastic shell

Acoustic droplet vaporization (ADV) is a new approach to generate vapor bubbles that have potentially broad medical applications. ADV-generated bubbles can be used as contrast agents in acoustic imaging, as drug carriers to deliver drugs to particular targets, and also in embolotherapy, thermal therapy, and histotripsy. However, despite much progress, ADV dynamics have still not been well understood and properly modeled. In this paper, we present a theoretical study of ultrasound-induced evaporation of a droplet encapsulated by a shell. The main emphasis of this theoretical study is on a proper description of the supercritical state occurring after bubble collapse. For this purpose, an isentropic equation of state for a van der Waals gas is used to describe the bubble behavior in the supercritical state. Sensitivity of the vaporization process is investigated for different acoustic and geometrical parameters and mechanical properties of the shell. Results show that the value of the minimum pressure required for direct vaporization (without any oscillatory behavior) depends on shell elasticity and initial size of the droplet, especially at high frequencies (greater than 2[MHz]). Moreover, it has been shown that applying an acoustic wave with proper phase such that thermal equilibrium of the bubble temperature with the surrounding liquid is attained, results in direct vaporization at lower acoustic pressure.

Blood-Brain Barrier Opening by Individualized Closed-Loop Feedback Control of Focused Ultrasound.

Objective and Impact Statement. To develop an approach for individualized closed-loop feedback control of microbubble cavitation to achieve safe and effective focused ultrasound in combination with microbubble-induced blood-brain barrier opening (FUS-BBBO). Introduction. FUS-BBBO is a promising strategy for noninvasive and localized brain drug delivery with a growing number of clinical studies currently ongoing. Real-time cavitation monitoring and feedback control are critical to achieving safe and effective FUS-BBBO. However, feedback control algorithms used in the past were either open-loop or without consideration of baseline cavitation level difference among subjects. Methods. This study performed feedback-controlled FUS-BBBO by defining the target cavitation level based on the baseline stable cavitation level of an individual subject with "dummy" FUS sonication. The dummy FUS sonication applied FUS with a low acoustic pressure for a short duration in the presence of microbubbles to define the baseline stable cavitation level that took into consideration of individual differences in the detected cavitation emissions. FUS-BBBO was then achieved through two sonication phases: ramping-up phase to reach the target cavitation level and maintaining phase to control the stable cavitation level at the target cavitation level. Results. Evaluations performed in wild-type mice demonstrated that this approach achieved effective and safe trans-BBB delivery of a model drug. The drug delivery efficiency increased as the target cavitation level increased from 0.5 dB to 2 dB without causing vascular damage. Increasing the target cavitation level to 3 dB and 4 dB increased the probability of tissue damage. Conclusions. Safe and effective brain drug delivery was achieved using the individualized closed-loop feedback-controlled FUS-BBBO.

High intensity focused ultrasound and microbubbles induce targeted mild hyperthermia suitable for enhanced drug delivery

High intensity focused ultrasound (HIFU) can elevate the temperature leading to tissue ablation (&amp;gt;60 °C). Mild hyperthermia (39–45 °C) can enhance drug delivery such as in the activation of thermosensitive liposomes for chemotherapy and in radiotherapy. The addition of microbubbles during HIFU could greatly enhance the heating process and increase the treatment area while operating at lower intensities. Here, we present a parametric study of microbubble-enhanced mild hyperthermia with HIFU in ex vivo porcine liver. A 30-s treatment at 0.9 MHz, 372 000 cycles, 82% duty cycle was used, and at estimated non-derated (water) pressures of 0.63, 1.25, 2.50 and 3.75 MPa. The microbubbles were injected locally in the treatment area. The measured temperature rise was greater with microbubbles than without at low acoustic pressures (0.63 and 1.25 MPa). At higher pressures (2.50 and 3.75 MPa), the presence of microbubbles did not have an effect on the measured temperature rise possibly due to inertial cavitation. Our results agree with predictions from a numerical model that solves the coupled acoustic-bubble flow fields with a two-way coupled Euler–Lagrange model. Future work will utilize the numerical model and new measurements to optimize targeted mild hyperthermia before applying it to enhanced drug delivery.

Safety of Image-Guided Treatment of the Liver with Ultrasound and Microbubbles in an in Vivo Porcine Model

Ultrasound and microbubbles are useful for both diagnostic imaging and targeted drug delivery, making them ideal conduits for theranostic interventions. Recent reports have indicated the preclinical success of microbubble cavitation for enhancement of chemotherapy in abdominal tumors; however, there have been limited studies and variable efficacy in clinical implementation of this technique. This is likely because in contrast to the high pressures and long cycle lengths seen in successful preclinical work, current clinical implementation of microbubble cavitation for drug delivery generally involves low acoustic pressures and short cycle lengths to fit within clinical guidelines. To translate the preclinical parameter space to clinical adoption, a relevant safety study in a healthy large animal is required. Therefore, the purpose of this work was to evaluate the safety of ultrasound cavitation treatment (USCTx) in a healthy porcine model using a modified Philips EPIQ with S5-1 as the focused source. We performed USCTx on eight healthy pigs and monitored health over the course of 1 wk. We then performed an acute study of USCTx to evaluate immediate tissue damage. Contrast-enhanced ultrasound exams were performed before and after each treatment to investigate perfusion changes within the treated areas, and blood and urine were evaluated for liver damage biomarkers. We illustrate, through quantitative analysis of contrast-enhanced ultrasound data, blood and urine analyses and histology, that this technique and the parameter space considered are safe within the time frame evaluated. With its safety confirmed using a clinical-grade ultrasound scanner and contrast agent, USCTx could be easily translated into clinical trials for improvement of chemotherapy delivery. This represents the first safety study assessing the bio-effects of microbubble cavitation from relevant ultrasound parameters in a large animal model.

Microbubble-Enhanced Heating: Exploring the Effect of Microbubble Concentration and Pressure Amplitude on High-Intensity Focused Ultrasound Treatments

High-intensity focused ultrasound (HIFU) is a non-invasive tool that can be used for targeted thermal ablation treatments. Currently, HIFU is clinically approved for treatment of uterine fibroids, various cancers, and certain brain applications. However, for brain applications such as essential tremors, HIFU can only be used to treat limited areas confined to the center of the brain because of geometrical limitations (shape of the transducer and skull). A major obstacle to advancing this technology is the inability to treat non-central brain locations without causing damage to the skin and/or skull. Previous research has indicated that cavitation-induced bubbles or microbubble contrast agents can be used to enhance HIFU treatments by increasing ablation regions and shortening acoustic exposures at lower acoustic pressures. However, little research has been done to explore the interplay between microbubble concentration and pressure amplitude on HIFU treatments. We developed an in vitro experimental setup to study lesion formation at three different acoustic pressures and three microbubble concentrations. Real-time ultrasound imaging was integrated to monitor initial microbubble concentration and subsequent behavior during the HIFU treatments. Depending on the pressure used for the HIFU treatment, there was an optimal concentration of microbubbles that led to enhanced heating in the focal area. If the concentration of microbubbles was too high, the treatment was detrimentally affected because of non-linear attenuation by the pre-focal microbubbles. Additionally, the real-time ultrasound imaging provided a reliable method to monitor microbubble activity during the HIFU treatments, which is important for translation to in vivo HIFU applications with microbubbles.

Focused Ultrasound Mechanical Disruption of Ex Vivo Rat Tendon.

Around 30 million tendon injuries occur annually in the U.S. costing $ 114 billion. Conservative therapies, like dry needling, promote healing in chronically injured tendons by inducing microdamage but have mixed success rates. Focused ultrasound (fUS) therapy can noninvasively fractionate tissues through the creation, oscillation, and collapse of bubbles in a process termed histotripsy; however, highly collagenous tissues, like tendon, have shown resistance to mechanical fractionation. This study histologically evaluates whether fUS mechanical disruption is achievable in tendons. Ex vivo rat tendons (45 Achilles and 44 supraspinatus) were exposed to 1.5-MHz fUS operating with 0.1-10 ms pulses repeated at 1-100 Hz for 15-60 s with peak positive pressures <89 MPa and peak negative pressures <26 MPa; other tendons were exposed to dry needling or sham. Immediately after treatment, tendons were flash-frozen and stained with hematoxylin and eosin (H&E) or alpha-nicotinamide adenine dinucleotide diaphorase ( α -NADH-d) and evaluated by two reviewers blinded to the exposure conditions. Results showed successful creation of bubbles for all fUS-treated samples; however, not all samples showed histological injury. When the injury was detected, parameter sets with shorter pulses (0.1-1 ms), lower acoustic pressures, or reduced treatment times showed mechanical disruption in the form of fiber separation and fraying with little to no thermal injury. Longer pulses or treatment times showed a combination of mechanical and thermal injury. These findings suggest that mechanical disruption is achievable in tendons within a small window of acoustic parameters, supporting the potential of fUS therapy in tendon treatment.

A Modeling Comparison of the Potential Effects on Marine Mammals from Sounds Produced by Marine Vibroseis and Air Gun Seismic Sources

Concerns about the potential environmental impacts of geophysical surveys using air gun sources, coupled with advances in geophysical surveying technology and data processing, are driving research and development of commercially viable alternative technologies such as marine vibroseis (MV). MV systems produce controllable acoustic signals through volume displacement of water using a vibrating plate or shell. MV sources generally produce lower acoustic pressure and reduced bandwidth (spectral content) compared to air gun sources, but to be effective sources for geophysical surveys they typically produce longer duration signals with short inter-signal periods. Few studies have evaluated the potential effects of MV system use on marine fauna. In this desktop study, potential acoustic exposure of marine mammals was estimated for MV and air gun arrays by modeling the source signal, sound propagation, and animal movement in representative survey scenarios. In the scenarios, few marine mammals could be expected to be exposed to potentially injurious sound levels for either source type, but fewer were predicted for MV arrays than air gun arrays. The estimated number of marine mammals exposed to sound levels associated with behavioral disturbance depended on the selection of evaluation criteria. More behavioral disturbance was predicted for MV arrays compared to air gun arrays using a single threshold sound pressure level (SPL), while the opposite result was found when using frequency-weighted sound fields and a multiple-step, probabilistic, threshold function.

Nonlinear dynamics and bifurcation structure of ultrasonically excited lipid coated microbubbles

In many applications, microbubbles (MBs) are encapsulated by a lipid coating to increase their stability. However, the complex behavior of the lipid coating including buckling and rupture sophisticates the dynamics of the MBs and as a result the dynamics of the lipid coated MBs (LCMBs) are not well understood. Here, we investigate the nonlinear behavior of the LCMBs by analyzing their bifurcation structure as a function of acoustic pressure. We show that, the LC can enhance the generation of period 2 (P2), P3, higher order subharmonics (SH), superharmonics and chaos at very low excitation pressures (e.g. 1 kPa). For LCMBs sonicated by their SH resonance frequency and in line with experimental observations with increasing pressure, P2 oscillations exhibit three stages: generation at low acoustic pressures, disappearance and re-generation. Within non-destructive oscillation regimes and by pressure amplitude increase, LCMBs can also exhibit two saddle node (SN) bifurcations resulting in possible abrupt enhancement of the scattered pressure. The first SN resembles the pressure dependent resonance phenomenon in uncoated MBs and the second SN resembles the pressure dependent SH resonance. Depending on the initial surface tension of the LCMBs, the nonlinear behavior may also be suppressed for a wide range of excitation pressures.

Safe Focused Ultrasound-Mediated Blood-Brain Barrier Opening and Repair is Not Mediated by Tight Junction Degradation

Focused ultrasound (FUS) with microbubbles opens the blood-brain barrier (BBB) to allow targeted drug delivery into the brain; however, the mechanisms of BBB opening and the response of the neurovascular unit at either low acoustic pressures, known to open the BBB transiently, or high pressures that cause brain damage, remain unclear. Employing a transgenic mouse strain where tight junctions (TJs) are labelled with eGFP, we examined TJ strand morphology at 1 and 72 hours post-FUS at both low and high pressures. We find that transient BBB opening due to FUS is not caused by TJ strand damage, unless FUS is used at high pressures, and this is localized primarily in arterioles and capillaries. At high pressures, TJ strands are obliterated and remain unrepaired even at 72 hours, allowing for fibrinogen passage, and persistent microglial activation. Our findings suggest that at low safe pressures, upregulation of transcytosis may likely increase transiently BBB permeability to deliver therapeutics to the brain.

Twisting waves increase the visibility of nonlinear behaviour

Nonlinear behaviour for acoustic systems is readily measured at high acoustic pressures in gasses or bulk materials. However, at low acoustic pressures nonlinear effects are not commonly observed. We find that by phase structuring acoustic beams, one observes evidence of nonlinear behaviour at an acoustic pressure of 66.78 dB lower than non-structured beams in room temperature air. A bespoke 28-element ultrasonic phased array antenna was developed to generate short pulses that carry orbital angular momentum and are propagated over a short air channel. When sampling small areas of the wavefront, we observed a distinctive change in the frequency components near phase singularities. At these phase singularities the local propagation path is screwed, resulting in the collection signals from pulses travelling along different paths across the aperture of a microphone. The usually negligible frequency chirping that arises from nonlinear behaviour in air interfere at these singularity points and produce a distinctive distortion of the acoustic pulse. Simple physical movement in the system or super-sonic wave speeds do not yield similar results. Such distortions in measured frequency response near phase singularities could lead to errors for SONAR or acoustic communication systems, where received signals are integrated over a finite-area detector. With further development this behaviour could potentially lead to accurate measurement techniques for determining a material’s nonlinear properties at lower acoustic pressure.

Theoretical investigation of the low frequency fundamental mechanism of the objective occlusion effect induced by bone-conducted stimulation.

The objective occlusion effect induced by bone-conducted stimulation refers to the low frequency acoustic pressure increase that results from occluding the ear canal opening. This phenomenon is commonly interpreted as follows: the bone-conducted sound "leaks" through the earcanal opening and is "trapped" by the occlusion device. This instinctive interpretation misrepresents the fundamental mechanism of the occlusion effect related to the earcanal impedance increase and already highlighted by existing electro-acoustic models. However, these models simplify the earcanal wall vibration (i.e., the origin of the phenomenon) to a volume velocity source which, in the authors' opinion, (i) hinders an exhaustive comprehension of the vibro-acoustic behavior of the system, (ii) hides the influence of the earcanal wall vibration distribution, and (iii) could blur the interpretation of the occlusion effect. This paper analyzes, illustrates, and interprets the vibro-acoustic behavior of the open and occluded earcanal using an improved finite element model of an outer ear in conjunction with an associated electro-acoustic model developed in this work. The two models are very complementary to dissect physical phenomena and to highlight the influence of the earcanal wall vibration distribution, characterized here by its curvilinear centroid position, on the occlusion effect.

The history force on bubbles translational motion in an acoustic field.

In this study, the translational trajectory of bubble in an ultrasonic standing wave at 22.4kHz was observed using an imaging system with a high-speed video camera. This allowed the velocities of bubble be measured when the acoustic pressure at 20kPa, 40kPa and 60kPa, which applied to indirectly measured the history force by using the acoustic and hydrodynamic forces balance model. It shown that bubbles driven at low acoustic pressure, the history force close to zero, and with the pressure increase the history force change to large, the ratio of FH/FQS from 0.33 at 40kPa to 1.73 at 60kPa, the result is different with prior research when the Reynolds numbers is large, and useful in the understanding of bubble moments in an acoustic field.