Low Temperature Storage Research Articles

Pengaruh Aplikasi Edible Coating Gel Lidah Buaya dan Kitosan terhadap Mutu Buah Stroberi pada Penyimpanan Suhu Ruang dan Suhu Rendah

Strawberries are nonclimateric fruits harvested in the final stages of ripening, so they are highly damaged due to cellular fragility, high respiration rate, and susceptibility to fungal growth. This study aims to test the effectiveness of edible coating of aloe vera gel and chitosan to maintain the quality and shelf life of strawberries. The study was carried out by experimental method with split plot by group random design. Storage temperature treatment as the main plot consists of two treatment levels, namely 25±2o C and 4±1o C, while the concentration of edible coating combination as a plot consists of five treatment levels, namely control without edible coating, aloe vera gel 40%, chitosan 2%, aloe vera gel 40% + chitosan 2%, and aloe vera gel 20% + chitosan 1%. Quality attributes including weight loss, fruit hardness, total dissolved solids, decay index, and shelf life are observed until total deterioration occurs. The results showed that at a storage temperature of 25±2° C, chitosan 2% treatment was more able to maintain the quality attributes of strawberries compared to other treatments. Meanwhile, at a storage temperature of 4±1°C, edible coating treatment of 40% aloe vera gel and 40% aloe vera gel + 2% chitosan which more effectively maintains the quality attributes of strawberries. However, for shelf life parameters, only the temperature factor of the storage room affects the length of shelf life of strawberries. The longest shelf life is achieved at low temperature storage (4±1°C) with edible coating treatment of 40% aloe vera gel + 2% chitosan (13.33 days). However, according to the LSD test at a real level of 5%, this value was not significantly different from other edible coating treatments stored at low temperatures, including the lowest shelf life that occurred in controls without edible coatings (10.67 days).

Intermetallic Compounds for Hydrogen Storage: Current Status and Future Perspectives.

Intermetallic compounds are an emerging class of materials with intriguing hydrogen activation and storage capabilities garnering attention for their application in low-temperature hydrogen storage and metal hydride batteries. However, none of the existing intermetallic compounds have met the gravimetric hydrogen storage capacity target of 5.5wt.%. Some A2B-type intermetallic compounds, like Mg2Ni, Mg2Co, and Mg2Fe, approach this target but require high temperatures for hydrogen desorption limiting their use in low-temperature hydrogen storage and automotive applications. Conversely, some intermetallic compounds like ZrV2 and LaNi5, exhibit hydrogen desorption at ambient conditions but with comparatively lower hydrogen storage capacities. This review article provides a comprehensive account of the different types of intermetallic compounds, their synthesis using different solidification-based and solid-state diffusion-based approaches, and their metallurgical and structural properties. It examines the complex interdependencies between the structural parameters of intermetallic compounds and hydrogen storage performance. A definitive but non-linear correlation is identified between void volume and gravimetric hydrogen storage capacities while lattice structure, evolution of lattice structure with hydrogen absorption, enthalpy of hydride formation, and hydrogen activation reactivities of intermetallic compounds are identified as critical parameters governing hydrogen storage performance.

Changes in the physicochemical properties of yellow pitahaya (Hylocereus megalanthus) during storage

Fresh fruits are essential for their flavor and nutritional value, and storage is one factor that decreases their shelf life. Low temperature or cold storage is a strategy to prolong the shelf life and maintain quality. Yellow pitahaya (Hylocereus megalanthus) was collected during the 2023 season, during May in the district of Magdalena (Chachapoyas, Amazonas, Peru). This study evaluated the physicochemical properties of yellow pitahaya stored at different conditions (room temperature, 2°C, and 4°C) every 15 days: Water activity, pH, total soluble solids (TSS), titratable acidity (TA), maturity index, antioxidant activity (by DPPH and ABTS) were also determined. The results showed significant changes in the physicochemical properties of pitahaya stored at different temperatures. The pitahaya stored at room temperature had higher water activity than samples stored at 2°C and 4°C, decreasing their solid content. The color of pitahaya also varied with the storage temperature, being 2°C ideal for maintaining the color. Therefore, storage at low temperatures slows down the degradation of the fruit, although precautions should be taken due to its susceptibility to physiological changes. Proper storage of pitahaya is essential to maintain its freshness and nutritional value.

Effects of Postharvest SO2 Treatment on Longan Aril Flavor and Glucosinolate Metabolites

SO2 fumigation treatment (commonly known as sulfur treatment, ST) is a key method in the postharvest preservation of imported and exported fresh longan fruits, effectively reducing pericarp browning and enhancing color. Nonetheless, distinctive aromas, often referred to as “sulfur flavor”, may develop in the aril during the extended preservation period. This study employed “Caopu” longan as the test material and patented SO2-releasing paper (ZL201610227848.7) as a treatment to perform a 35-day low-temperature (5 °C) storage of the fruit. The changes in glucosinolates (GSLs) and associated metabolites in the aril of treated fruit (ST) were examined utilizing ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) detection and widely targeted metabolomics technology. The findings indicated that following 35 days of storage, nearly all control (CK) fruit pericarp turned to brown, resulting in an edible fruit rate of 75.41% and a commercial fruit rate of 0%. In contrast, the treated (ST) fruit demonstrated an edible fruit rate and a commercial rate of 99.44%, while the pericarp color changed from dark yellow-brown to light earthy yellow. The sulfur-containing metabolites identified in longan fruit aril predominantly consist of amino acids and their derivatives (60.44%), followed by alkaloids (15.38%), nucleotides and their derivatives (1.10%), and other types (23.08%), which include GSLs. SO2 treatment significantly reduced the content of oxidized glutathione in fruit aril but increased the content of GSLs and related amino acids and their derivatives. Via screening, 19 differential sulfur-containing metabolites were obtained between ST and CK, including 11 GSLs. The identified differential metabolites of GSLs were all increased, primarily comprising aliphatic GSLs, such as 1-hydroxymethyl glucosinolate, 2-Propenyl glucosinolate (Sinigrin), and 4-Methylsulfinylbutyl glucosinolate (Glucoraphanin). Pathway analysis showed that these differential metabolites were mainly involved in coenzyme factor synthesis, cysteine and methionine metabolism, and amino acid synthesis, among other pathways. To the best of our knowledge, this is the first study to reveal the causes of the special flavor of longan aril after SO2 treatment, which is a great concern for longan consumers. Moreover, this study provides a scientific basis for exploring the reasons and mechanisms for the development of the sulfur flavor in the SO2-treated fruits during postharvest storage.

Underground Hydrogen Storage: Comparison of High-pressure Hydrogen, Liquid Hydrogen, and Ammonia

One of the alternatives for effective storage of irregularly produced renewable energy is hydrogen energy. In order to realize a hydrogen-based society, not only environmentally friendly production of hydrogen but also effective storage is very important. Underground hydrogen storage technology is a technology that has evolved from the technology for storing natural gas underground, and includes waste gas fields, salt domes, aquifers, and rock cavities. When stored underground, hydrogen is converted into high-pressure gaseous hydrogen, liquefied hydrogen, and ammonia. Liquefied hydrogen requires extremely low storage temperatures, and ammonia is a toxic substance that requires separate handling, and energy loss occurs during the conversion process. To compensate for this, research on liquefied hydrogen, such as multilayer insulation technology, is being conducted. Ammonia has successfully extracted high-purity hydrogen by developing a membrane reactor. Ammonia toxicity can be prevented by strengthening leak detection and blocking facilities. Among these, ammonia was found to be the most suitable for underground storage in terms of economic feasibility, environment, and commercialization.

Sodium nitroprusside applications modulate cell wall degrading enzymes and preserves the postharvest quality of cold-stored mangoes

ABSTRACT Mango fruit undergoes various physiochemical changes after its harvest that affect consumer acceptability. The present research work was carried out to evaluate the preservation effect of sodium nitroprusside (SNP) treatments on the postharvest quality of mango cv. Langra fruits at 12 ± 1°C for 35 days. The results revealed that SNP 1.0 mM treated fruits exhibited reduced weight loss (6.94%), decay incidence (8.05%), and activities of cell wall degrading enzymes like polygalacturonase and pectin methyl esterase in comparison to uncoated fruits on the last day of storage. Furthermore, the treatment maintained higher fruit firmness (56.77 N) along with fruit quality parameters like soluble solid content (16.24%), titratable acidity (0.20%), starch (4.99%), and, total flavonoid content (12.92 mg/100 g). Overall, SNP 1.0 mM combined with low-temperature storage was proved to be the most effective in extending the shelf life and preserving the quality characteristics of mango fruits.

Effect of 1-Methylcyclopropene and Chitosan Treatment on Biochemical and Antioxidant Properties of Avocado (Persea Americana Mill.) Fruit During Low and Ambient Temperature Storage

Effect of 1-Methylcyclopropene and Chitosan Treatment on Biochemical and Antioxidant Properties of Avocado (Persea Americana Mill.) Fruit During Low and Ambient Temperature Storage

Effect of individual quick freezing treatment and cold storage on the host egg (Lepidoptera: Saturniidae) quality for the production of the parasitoid Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae)

BackgroundThe augmentative release of the parasitoid species Anastatus japonicus Ashmead (Hymenoptera: Eupelmidae) is essential for managing the recent invasion of litchi stink bugs (Tessaratoma papillosa (Drury)) (Hemiptera: Tessaratomidae) in Taiwan. The mass release program in the spring targets this univoltine pest’s reproduction cycle. Low-temperature storage for the host eggs and parasitoids is required to maximize the production for the annual release program. This study aimed to enhance the shelf life of Samia cynthia (Lepidoptera: Saturniidae) eggs using the combination of refrigeration and individual quick freezing (IQF) as a storage treatment. Storage of A. japonicus in the IQF-treated S. cynthia eggs was evaluated to determine the optimal production process during the short preparation window before the release.ResultsThe influences of cold storage and IQF treatment on the host eggs were evaluated for different storage durations. The host quality was determined according to the emergence rate and female ratio of A. japonicus. The study’s results demonstrated that the host quality of S. cynthia eggs did not change significantly compared to the control group after 28 d of refrigeration at 7 °C. IQF treatment extended S. cyathia’s shelf life to 5 months without significantly affecting the host quality. Applying IQF at different developmental stages of the host eggs affected the parasitism rates of A. japonicus. Significantly higher emergence rates of A. japonicus were recorded in the eggs treated during the initial development (D0 to D2). However, storing parasitoids in the IQF-treated eggs under the current storage condition for one month at 12 °C significantly impacted the parasitoid’s emergence rate.ConclusionsThe results of this study highlight the potential benefits of the individual quick freezing technique. This technique didn’t alter the egg quality of S. cynthia, a crucial factor for the mass production of A. japonicus. The IQF treatment extends the shelf life of the host eggs to 5 months when stored at − 20 °C, a significant improvement compared to the 4-week shelf life without IQF treatment. This technology offers a promising and efficient approach for the mass production of A. japonicus and supports field releases. The treatment allowed for the stockpiling of host eggs during the off-season, which can help expedite the mass production of A. japonicus to fit an eight-week production window.

Clinical Test Comparison for Improved Diagnosis of Mucormycosis

Abstract Mucormycosis is a rapidly progressive and invasive fungal infection caused by members of the order Mucorales. Proper treatment relies on a timely diagnosis. However, traditional fungal culture takes several days to weeks and has low sensitivity for recovering Mucorales, as these organisms are sensitive to specimen preparation, including tissue homogenization, and low storage temperatures. More rapid and sensitive diagnostic methods are needed to promptly detect infection. Our laboratory previously developed a real-time PCR assay that detects the most common genera associated with mucormycosis to help improve diagnosis. The assay was analytically validated for a variety of specimen types including serum, tissue (both fresh and FFPE), respiratory secretions and body fluids. Little is known about the agreement between our laboratory developed PCR and other detection methods such as culture or sequencing. We reviewed 6 years of laboratory data to assess overall PCR positivity rate, positivity rate across sample types, and agreement with culture and/or pan-fungal sequencing. We also calculated turnaround time to results to gauge the diagnostic value of Mucorales PCR. Test comparisons with fungal culture and/or pan-fungal sequencing were performed if testing was ordered within 30 days of each other. In our study, we reviewed 3,804 total PCR orders for 2,195 patients. The overall positivity rate of Mucorales PCR orders was 3.81% and varied among different sample types, with fresh tissue and body fluid having the highest positivity rates (18.11% total tissue (33.3% lung tissue) and 10.94% all body fluid (12.5% pleural fluid)) and sputum and CSF having the lowest positivity rates (<1% positivity for both). In a cohort of 428 patients with fungal culture and/or sequencing also performed, 29 patients tested positive for Mucorales by at least one method. PCR detected Mucorales on average 7.83 days sooner than fungal culture (average time to positive result: 3.65 vs. 11.47 days). In patients with culture- or sequencing-confirmed Mucorales infection, PCR had a 100% agreement when comparing shared samples from the same source and collection time. Mucorales PCR was also able to detect infection in 10 (34.5%) other patients who were culture-negative for Mucorales. Our findings suggest that PCR could be a valuable diagnostic tool for suspected mucormycosis, especially tissue invasive disease. PCR increased case detection by 34.5% with a faster turnaround time than culture with lesser cost than sequencing. The main limitation of our study was lack of access to patient information to assess pretest probability of Mucormycosis.

Germination of soybean seeds after low-temperature storage and its dependence on weather and climate conditions in the places of reproduction of accessions

Background. Soybean (Glycine max (L.) Merr.) is a microbiotic whose seeds quickly lose germination in uncontrolled conditions at room temperatures. This determines the need to preserve accessions in special conditions. Low temperature storage (LTS) allows to maintain high seed germination ability, but not for all accessions. Therefore, it is relevant to assess the results of LTS at the VIR Genebank and to make an attempt to determine the best geographical conditions for growing plants and harvesting seeds that will be subjected to LTS. Materials and methods. The work was carried out on 312 soybean accessions from the VIR collection, originating from almost the entire area of soybean cultivation. The accessions used in the research were reproduced at three VIR experiment stations (ES), namely Adler ES, Kuban ES (Krasnodar Territory) and the Far East ES (Primorsky Territory). The seeds were harvested in 1999 to 2017 and stored in sealed foil laminated bags at –10°C from 2002 to 2021. The accessions were removed from LTS and germination assessed in 2022. Results. At the beginning of LTS, seed germination (Gi) ranged from 12 to 100% (averaging 79.1%), while that after LTS (Gr) ranged from 1 to 97% (57.8% on average). The retention of germination ability was expressed as the germination index (GI = Gr / Gi), which ranged from 0.02 to 1.73 (an average of 0.72). All the indicators of germination in the range of up to 20 years of LTS were not associated with the duration of LTS and with the number of years before LTS (0.5-4 years in our research). The climatic conditions characteristic of the place of reproduction had a significant impact on Gi and Gr, but not on GI. The average Gi of seeds obtained at the Adler ES and Kuban ES did not differ significantly (77.4% and 75.7%), while that of seeds harvested at the Far East ES was significantly higher (84.0%). Gr of the accessions from the Far East ES (64.3%) was also greater than that of accessions from the Kuban ES (52.2%) and Adler ES (57.2%). The excessively high sum of temperatures above 10°C (recorded in some years at all stations) reduced Gi, Gr but had little effect on GI. The highest values of Gi, Gr, and GI were demonstrated by the accessions maturing in 101-120 days. Additionally drying of the most late-ripening accessions (later than 140 days) in sheaves under an awning yielded seeds with high germination ability and good LTS tolerance. Conclusion. The seeds from different places of reproduction of accessions differed in germination before and after LTS, and, on an average, the highest values were demonstrated by the seeds harvested at the Far East ES, where the temperature regime is closer to the optimal one for soybeans. The seed germination index showed relative independence from the conditions of reproduction of accessions, including the climate and weather conditions, as well as the initial germination ability and LTS duration.

Investigation of heat leakage calculations and experiments for a wide-mouth liquid nitrogen tank

ABSTRACT With the continuous development of biomedicine, the long-term low-temperature storage of biological samples has become essential. In order to meet the demand for long-term storage of liquid nitrogen to keep samples safe, a study on the evaporated gas recycling storage of liquid nitrogen tanks will be carried out. This research utilizes new approach to accurately calculate the heat leakage of the liquid nitrogen tank. The experimental results show that the theoretical heat leakage is 6.16 W, and the actual heat leakage is 7.07 W. By incorporating the insulating plug, the actual heat leakage was reduced by 61.2%, decreasing to 2.74 W, and the calculated heat leakage is 2.47 W. The error margin in both cases was approximately 10%. Furthermore, in contrast to existing investigations, this experiment with the 8W@77K pulse tube cryocooler for recovering nitrogen is conducted under environmental pressure rather than overpressure, and it also takes into account the sensible heat issues caused by ambient pressure. The ratio of 1.81 W of sensible heat to 2.74 W of latent heat is 2:3, indicating that under ambient pressure conditions, the impact of sensible heat cannot be ignored. The key improvement methods are given to improve the success rate of the experiment. The research results provide a useful reference for the re-cycling and storage of liquid nitrogen.

Exploring the possible mechanisms of X-rays treatment for retention aroma volatiles in shiitake mushrooms during low temperature storage

X-rays irradiation has been demonstrated to effectively preserve the freshness of edible fungi and delay the loss of aroma during storage. In this study, shiitake mushrooms were irradiated with X-rays dose of 0.5 kGy and stored at 2 °C for 35 days. Non-irradiated mushrooms were recorded as control group. Results indicated that 0.5 kGy X-rays treatment preserved the flavor quality by exhibiting higher volatile substance content in shiitake mushrooms. X-rays treatment promoted the activities of lipoxygenase (LOX) and alcohol dehydrogenase (ADH) and oxidation of linoleic acid. In addition, the degradation of methionine and cysteine was facilitated by X-rays. Higher enzymes activities for γ-glutamyl transpeptidase (GGT) and cystine sulfoxide lyase (CS lyase) were found in 0.5 kGy X-rays irradiated mushrooms. These findings suggest that the retention of mushroom flavor by X-rays treatment is closely related to fatty acid metabolism, sulfur-containing amino acid metabolism, and lentinic acid metabolism.

Failure Mechanism Analysis of 1.2 kV SiC MOSFETs Under Low-Temperature Storage, Power Cycling, and Short-Circuit Interactions

Failure Mechanism Analysis of 1.2 kV SiC MOSFETs Under Low-Temperature Storage, Power Cycling, and Short-Circuit Interactions

Effect of storage conditions on host egg suitability and the reproductive fitness of Oobius agrili (Hymenoptera: Encyrtidae), an egg parasitoid of the invasive emerald ash borer (Agrilus planipennis) (Coleoptera: Buprestidae).

Oobius agrili Zhang and Huang (Hymenoptera: Encyrtidae) is an important egg parasitoid of the emerald ash borer (EAB), Agrilus planipennis Fairmaire (Coleoptera: Buprestidae). Methods for laboratory-rearing O. agrili have been developed but its mass-production depends on the continuous production and storage of freshly laid EAB eggs as well as diapaused parasitoid progeny (inside parasitized EAB eggs). The purpose of this study was to determine optimal environmental conditions for long-term storage of host eggs as well as diapaused parasitoid progeny. Fresh host eggs and diapaused parasitoid progeny were stored at two low storage temperatures (1.7 and 12.8 °C) and three levels of relative humidity (low ~31%, medium ~74%, and high ~99.9%) for various length of time (15-270 days) and then evaluated for host egg suitability and the reproductive fitness of stored parasitoid progeny. EAB eggs were stored for approximately 30 days without significant reduction of their viability and suitability to O. agrili parasitism at low storage temperatures under high and medium relative humidity. Neither storage temperature or humidity had any significant effects on adult parasitoid emergence for storage durations of up to 270 days. When storage durations were over 120 days, however, both adult parasitoid longevity and fecundity declined approximately 20-30% across all temperature and humidity treatments. Relevance of findings to mass-production and storage of O. agrili for biocontrol is discussed.

Exogenous nitric oxide and hydrogen sulfide as biotechnological tools for enhancing plant adaptation to cold

The challenge of increasing plant tolerance to low temperatures is still relevant despite the upward trend in the average temperatures on Earth. Sharp temperature fluctuations during growing periods and cultivation of thermophilic crops in more northern latitudes increase the likelihood of plants being damaged by cold. Physiologically active substances, primarily hormones and signalling molecules, are considered to be effective for increasing plant tolerance to extreme temperatures. In the last decade, among such compounds, researchers' and practitioners' considerable attention has been paid to gasotransmitters – gaseous substances that are synthesized by living organisms and perform signalling functions. The review analyses the molecular mechanisms of stress protective effects of two major gasotransmitters – nitric oxide (NO) and hydrogen sulfide (H2S). In particular, the physiological effects of gasotransmitters that are attributed to their ability to cause post-translational modifications of numerous target proteins as well as those related to their functional interactions with other signaling intermediaries, primarily ROS and calcium ions, are discussed. The available data on the actions of donors of these gasotransmitters on thermophilic and cold-tolerant plants are summarized. Particular attention is paid to the influence of exogenous NO and H2S on the antioxidant system in plants, their ability to accumulate osmolytes and other low-molecular compounds with multifunctional stress protective effects. Data on the influence of gasotransmitters on fruits during low-temperature storage are analysed separately. It is emphasized that increased storability of fruits under the influence of NO and H2S donors is largely controlled by the ability of both gasotransmitters to inhibit ethylene synthesis. It was concluded that exogenous nitric oxide and hydrogen sulfide could be effective tools for increasing tolerance of different plant taxon’s to low temperatures and used in crop production and storage technologies for fruits and vegetables.

Interface stabilization strategy realizing low-temperature sodium storage in Sb anode prepared by ball milling method

Antimony (Sb) is an attractive anode material for sodium-ion batteries (SIBs) owing to its low operating potential, low cost, and high theoretical capacity. However, the large volume expansion of Sb during the Na alloying/dealloying process, which greatly limits its application in SIBs. Simultaneously, as the temperature decreases, the formation of a poor coating on the anode surface significantly increase the difficulty of Na+ transmission. Herein, Sb-graphite-NaF (SCF) ternary composites were prepared by an easy to operate and low-cost ball milling method, which significantly improved the cycling stability and extended to the operation temperature of Sb-base SIBs. A small amount of NaF induces the formation of an inorganic-dominated NaF-rich stable solid electrolyte interphase (SEI) on the Sb anode surface, which can cooperate with graphite to inhibit volume expansion of the Sb particles during sodium storage, enhance conductivity and accelerate Na+ diffusion at room and low temperatures. As a result, the SCF electrode with propylene carbonate-base electrolyte delivered a discharge capacity of 458.4 mAh g−1 after 160 cycles at 500 mA g−1 at 20 °C. When matched with diethylcarbonate-based electrolyte at −20 °C, it had a specific capacity of 299.8 mAh g−1 after 120 cycles and a capacity retention rate of 86.2 %. This study shows that the interface stabilization strategy with a suitable electrolyte can improve the cycling stability of anode materials, provide a new idea for constructing NaF-rich SEI, and a new approach for applying Sb-based materials in low-temperature SIBs.

Addition of Cryoprotectant DMSO Reduces Damage to Spermatozoa of Yellow Catfish (Pelteobagrus fulvidraco) during Cryopreservation: Ultrastructural Damage, Oxidative Damage and DNA Damage.

Spermatozoa cryopreservation protocols have been established for yellow catfish (Pelteobagrus fulvidraco), but cryopreservation can still cause cellular damage and affect spermatozoa viability and fertility. Therefore, the aim of this paper was to evaluate the effects of adding or not adding cryoprotectants during low-temperature storage on the ultrastructural damage, oxidative damage, and DNA damage of thawed yellow catfish spermatozoa. The mixed semen of three male yellow catfish was divided into a fresh spermatozoa group, a frozen spermatozoa group (DMSO+) with a cryoprotectant (10% DMSO), and a frozen spermatozoa group without a cryoprotectant (DMSO-). Ultrastructural of the spermatozoa after thawing were observed under an electron microscope and the spermatozoa were assayed for SOD, MDA, and T-AOC enzyme activities, as well as for DNA integrity. In terms of movement parameters, compared with DMSO-, the addition of DMSO has significantly improved sperm motility, curve line velocity (VCL), and straight line velocity (VSL). The ultrastructural results showed that although thawed spermatozoa exhibited increased damage than fresh spermatozoa, 10% DMSO effectively reduced the damage to the plasma membrane, mitochondria, and flagellum of spermatozoa by cryopreservation, and most of the spermatozoa were preserved with intact structure. The results of oxidative damage showed that compared with frozen spermatozoa, 10% DMSO significantly increased the activities of SOD and T-AOC enzymes and clearly reduced the activity of the MDA enzyme. The antioxidant capacity of spermatozoa was improved, lipid peroxidation was reduced, and the oxidative damage caused by cryopreservation was mitigated. The DNA integrity of spermatozoa showed that 10% DMSO clearly reduced the DNA fragmentation rate. In conclusion, 10% DMSO can effectively reduce the ultrastructural damage, oxidative damage, and DNA damage of yellow catfish spermatozoa during cryopreservation; it can also further optimize the cryopreservation protocol for yellow catfish spermatozoa. Meanwhile, it also provides a theoretical basis for the future optimization of the cryopreservation protocols.

Impact of polysaccharide and lipid based edible coatings on storage life and quality of muskmelon (Cucumis melo) fruits during low temperature storage

Impact of polysaccharide and lipid based edible coatings on storage life and quality of muskmelon (Cucumis melo) fruits during low temperature storage

Low temperature conditioning and nitric oxide treatment reduced chilling injury and altered fatty acid metabolism of flat peaches

Low-temperature conditioning is a recognized method for alleviating chilling injuries in flat peaches. However, long-term low-temperature storage can mitigate volatile organic compounds, thereby limiting fruit availability. To improve the supply of flat peaches, we investigated the combined effects of low-temperature conditioning and nitric oxide treatment on various parameters including physiological quality, volatile organic compounds, fatty acids, associated enzymes, and genes involved in the fatty acid pathway with the transcriptome sequencing analysis. The results indicated that low-temperature conditioning coordinated with 10 µL L−1 nitric oxide treatment possessed higher firmness and vitamin C content and restricted the content of malonaldehyde and relative electrical conductivity, thus maintaining the overall quality of flat peaches. In the study, 10 µL L−1 nitric oxide was employed for subsequent experiments. Moreover, higher contents of volatile organic compounds were supported by increased contents of palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, alcohol dehydrogenase, hydroperoxide lyase, and up-regulated genes of LOX2, LOX3, LOX6, ADH2, and ADH3. In summary, nitric oxide treatment effectively facilitated the recovery of volatile organic compounds in flat peaches during cold storage. Therefore, low-temperature conditioning and nitric oxide treatment can be a promising strategy to prevent the loss of volatile organic compounds by modulating the fatty acid pathway and associated genes.

Physiological and Biochemical Effects of Intermittent Warming on 'Marmandi' Turning Tomato Fruits During Low-Temperature Storage

This experiment was conducted on the variety 'Marmandi' turning tomato fruits to investigate the effect of intermittent warming temperatures during extended low chilling temperature storage (21 days) on the subsequent ripening quality of tomato fruits. The quality was measured by assessing the quantitative amount of total phenolic compounds, chlorogenic acid, and ascorbic acid, as well as the development of fruit color and the evaluation of chilling injury (CI) symptoms. The results indicated that the amounts of total phenolic compounds and chlorogenic acid increased significantly during continuous low-temperature storage and after subsequent transfer to room temperature, with this increase being associated with significant loss of ascorbic acid. More frequent periods of intermittent warming (IW) during low-temperature storage were very effective in significantly decreasing the rate of accumulation of phenolic compounds, chlorogenic acid, and ascorbic acid losses. A significant reciprocal relationship between the amounts of chlorogenic acid and ascorbic acid was observed. Additionally, the results showed that as IW time increased, its effectiveness in retarding CI significantly improved.