The rapid modernization in China has aggravated the reduction of the traditional settlements and aroused concern about the protection and research. This study aims to examine the spatial-temporal variations Tujia traditional settlements in China and to delineate the driving mechanism of the settlement distribution. Previous studies have focused on the characteristics of settlements in provincial or smaller areas, providing lacked information regarding spatial distribution heterogeneity of Tujia traditional settlements in China. In this study, the spatial heterogeneity and influence factors of the distribution of traditional Tujia settlements were examined using the GIS platform and statistical methods. The results reveal that the spatial distribution of settlements exhibits clustering with the pattern of "scattered distribution in a large region, while concentrated in small areas". The settlements were generally built in low hills, gentle slopes, sunny slopes and low-relief terrain areas, with elevation, relief degree of land surface (RDLS), slope and aspect were the key factors affecting the distribution. In Song, Yuan, Ming and Qing dynasties, settlements showed significant clustering all through, though the location and number of clustering center kept changing. In this process, the history of the Tujia chieftain and the transportation and marketing lines of Sichuan salt had a profound influence on the historical evolution of the settlement.